Csor.00g312270 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g312270
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProtein kinase domain-containing protein
LocationCsor_Chr15: 1055870 .. 1059005 (+)
RNA-Seq ExpressionCsor.00g312270
SyntenyCsor.00g312270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGAACTGAGGCTACTGCTTCTTGTCTTGTGGCTTCTTTATTGTTTTGGGCTTCTGCAATAAATGGCTTGCTATCTCCCAAAGGTGTTAACTATGAAGGTAAGTAAGAGAAAAAGAGAACAAAATTCTTTTTCTTTCTGCAAGATGGGTGTGTCTGTTTAGGCCATTTTAAGATGGGAAATGCAGAATTTTCATGGGGGAAATGGTTTCATTTTTTCACTGTTTCATTTCAGTTCAAGCTCTGATGGGTATAAAAGCTTCACTTCAAGATCCCCATGGCGTGCTTGAGAATTGGGATGGAGACTCTGTTGACCCATGTAGTTGGACTATGGTTACTTGCTCCCCTGACAGCCTTGTCATTGGCCTGTTAATCCTCTTCTTCACCTTCTTCTTTAAGAATTTGAACTCAAAATTTGTAGATGAAGAGTTGGGAAAATGGAGGCAATCCACCATTGAAGTTCTGATTCATTTTTCTTTCTTTTGGTGGGTTTTGTACGTAATGCAGAGGGACTCCAAGTCAGAACCTGTCTGGGACTCTTTCTTCAAGCATTGGGAATTTGACAAACCTTCAAATTGTGTAAGTTAAAATGACAAGGCCTTTCTTTTCCTCTTTCTCCCCCTTGAAAATGGAGAAAAATAAGTAACCGAATTGGTTTGCATTTGCTCAATAGGCTGTTGCAGAACAACAATATTACGGGCCCAATCCCCCCAGAGCTTGAGAGGCTCTCAAAGCTCCAAACACTTGATCTATCAAGCAATTTCTTCACAGGGAAAATTCCTTCTTCTTTAGGCCATTTGAGAAGCCTACAGTACTTGTAAGCATCTTCCTATTATGCCTTTTGCTTGCTTTCCCTCAAATGTTTTAGTTTAATTTGGTCTGTTCTTCAATGATTTTAGGAGGCTTAACAACAACAGTCTTTCTGGGGTAATTCCAATGTCTGTAGCTAACATGACACAGCTTGTGTTTCTGTAAGTTCAAATCTTTTCAAATTCATCCATCTTGACGAAAGCTTGCTCTTGAATTTAGTTTCTTATTCTAAATTTGTTTGTAATGCAGCGATGTGTCTTATAATAATATCAGTGGCCCTCTCCCCAGATTGCCCAGCAAAACTTTCAAGTATATATCACTCTCTTTCCTCTGTATGTGTGTGTGTTTTCTGTGTGAAGTCTCACTCACGTAATATTTCTGATATTGCAGCATTGTTGGAAACCCTCTGATCTGTGCAACTGGGTCCGAGGTTGGTTGCCACGGTTCAACTCTAATGCCCATGTCCATGAACTTGAACAGTACACAAAGTAAGGTTCATGTAAATACGTTGCCATTGATGAATTCCTTCAGAGCCTTTCCAATTCAGTAGTTATTGTTTGTAAGAATTCTGTTCTCTTTGCTTCTTTAGCTGGTTTACCTGCTGTGAGATTGAAAAGTCATAAAATGGCCCTTACATTTGGCTTGAGCCTAGCTTGCCTATGTCTGATTGTTCTTGTATTTGGACTGTTCATATGGTGGCGAAGACGAAGCAATCGCCCGACGTTTTTCGATGTAAAAGGTAAATTGATTGGAGCATTGTACCAGTTTTTCTTCTCATGATTACTGTTAAATTTACGCTAATCCCAACATCTGAACTGAACGATAAGGCCAAACGTTAAGCTTCAGTATTGATTGTATTATCATTCTAATATATGTCCTATGCATCTTACTGCAATTGCAGACGGACAACATGAGGAAATTTCGCTTGGAAATCTGAGAAGGTTCCAATTTAGACAACTTCAGATAGCAACAAACAACTTCAGCAGCAAGAACATACTAGGAAAGGGAGGTTTTGGAAATGTTTACAAAGGAATTCTTTCAGATGGAACGGTTGTAGCTGTTAAAAGGCTCAAGGATGGCAATGCAAGTAGAGGTGATATCCAGTTTCAGACTGAAGTCGAGATGATCAGCCTAGCCGTGCACCGTCATCTCCTCCGACTGTACGGGTTTTGCAACACACCAACCGAAAGGATTCTTGTTTACCCGTATATGTCTAATGGAAGCGTTGCATCACGTCTCAAAGGTGGCCATTTTAAGAGCATCAATGAATATCCATCTATACATTTGATAACTGACCATATGCAGGCTACAATATCCAATGCCCGACACTGAAACACGAGAAAATTATCGGTTTCTTTTGCAGGAAAACCGGTATTAGACTGGGGAACAAGGAAGAGAATTGCTATAGGAGCTTCAAGGGGATTGTTATACCTCCATGAACAATGTGACCCAAAGATCATACATAGAGACGTTAAGGCTGCAAACATATTGCTCGACGACTACTGCGAGGCTGTCGTTGGAGATTTCGGGTTGGCAAAGCTTCTCGACCACCAAGATTCACATGTCACAACTGCTGTGAGAGGCACCGTGGGTCATATAGCTCCTGAGTATCTCTCGACTGGGCAGTCATCCGAGAAAACAGACGTTTTTGGATTCGGAATTCTACTCCTTGAACTGATCACAGGGCAGAAAGCCCTTGAGTTTGGCAAAACAGCTAATCAGAAAGGAGGCATTCTTGAATGGGTATGCATCACCATATAAAAACAATACACATATCTCATCTTTGTTATCCTTTGATTATTCTTAAGAACTTTTATAGCTTCTCAAAGTTTTCATTCAAGAGGGAGGAGAACACAACAAAATATGCCATTGTTATTGTGTTCCTCTCGAGCTTTCGACATCGATAAACCAAAGGAAGTTACGAATACGTGTCGATTGAAATAGGAAGGCATGGTTTGAGCATTAGAAGATGAAAATGAATGACACCGAGTTTATAATTACAGGTGAAGAAGATTCACCAGGAGAAGAAGCTGGAAGTGCTAGTGGACAAGGATCTGAAAGCCAACTATGACAGAGTTGAGCTAGAGGAGATGGTTCAAGTGGCTCTGTTGTGTACACAATACCAACCAGGACACAGACCGAAGATGTCAGAAGTTGTCAGAATGCTTGAAGGAGAAGGTCTTGCTGTCAGATGGGAAGCTTCTCAGAGAGTTGACTCAACAAAGTGCAAACCTCATGATTTCTCTTCATCTGACAGATACTCTGATCTCACAGATGACTCATCCTTGTTGGTCCAAGCCATGGAGCTCTCTGGTCCAAGGTGA

mRNA sequence

ATGAAGAGAACTGAGGCTACTGCTTCTTGTCTTGTGGCTTCTTTATTGTTTTGGGCTTCTGCAATAAATGGCTTGCTATCTCCCAAAGGTGTTAACTATGAAGTTCAAGCTCTGATGGGTATAAAAGCTTCACTTCAAGATCCCCATGGCGTGCTTGAGAATTGGGATGGAGACTCTGTTGACCCATGTAGTTGGACTATGGTTACTTGCTCCCCTGACAGCCTTGTCATTGGCCTAGGGACTCCAAGTCAGAACCTGTCTGGGACTCTTTCTTCAAGCATTGGGAATTTGACAAACCTTCAAATTGTGCTGTTGCAGAACAACAATATTACGGGCCCAATCCCCCCAGAGCTTGAGAGGCTCTCAAAGCTCCAAACACTTGATCTATCAAGCAATTTCTTCACAGGGAAAATTCCTTCTTCTTTAGGCCATTTGAGAAGCCTACAGTACTTGAGGCTTAACAACAACAGTCTTTCTGGGGTAATTCCAATGTCTGTAGCTAACATGACACAGCTTGTGTTTCTCGATGTGTCTTATAATAATATCAGTGGCCCTCTCCCCAGATTGCCCAGCAAAACTTTCAACATTGTTGGAAACCCTCTGATCTGTGCAACTGGGTCCGAGGTTGGTTGCCACGGTTCAACTCTAATGCCCATGTCCATGAACTTGAACAGTACACAAACTGGTTTACCTGCTGTGAGATTGAAAAGTCATAAAATGGCCCTTACATTTGGCTTGAGCCTAGCTTGCCTATGTCTGATTGTTCTTGTATTTGGACTGTTCATATGGTGGCGAAGACGAAGCAATCGCCCGACGTTTTTCGATGTAAAAGACGGACAACATGAGGAAATTTCGCTTGGAAATCTGAGAAGGCTCAAGGATGGCAATGCAAGTAGAGGTGATATCCAGTTTCAGACTGAAGTCGAGATGATCAGCCTAGCCGTGCACCGTCATCTCCTCCGACTGTACGGGTTTTGCAACACACCAACCGAAAGGATTCTTGTTTACCCGTATATGTCTAATGGAAGCGTTGCATCACGTCTCAAAGGAAAACCGGTATTAGACTGGGGAACAAGGAAGAGAATTGCTATAGGAGCTTCAAGGGGATTGTTATACCTCCATGAACAATGTGACCCAAAGATCATACATAGAGACGTTAAGGCTGCAAACATATTGCTCGACGACTACTGCGAGGCTGTCGTTGGAGATTTCGGGTTGGCAAAGCTTCTCGACCACCAAGATTCACATGTCACAACTGCTGTGAGAGGCACCGTGGGTCATATAGCTCCTGAGTATCTCTCGACTGGGCAGTCATCCGAGAAAACAGACGTTTTTGGATTCGGAATTCTACTCCTTGAACTGATCACAGGGCAGAAAGCCCTTGAGTTTGGCAAAACAGCTAATCAGAAAGGAGGCATTCTTGAATGGGTGAAGAAGATTCACCAGGAGAAGAAGCTGGAAGTGCTAGTGGACAAGGATCTGAAAGCCAACTATGACAGAGTTGAGCTAGAGGAGATGGTTCAAGTGGCTCTGTTGTGTACACAATACCAACCAGGACACAGACCGAAGATGTCAGAAGTTGTCAGAATGCTTGAAGGAGAAGGTCTTGCTGTCAGATGGGAAGCTTCTCAGAGAGTTGACTCAACAAAGTGCAAACCTCATGATTTCTCTTCATCTGACAGATACTCTGATCTCACAGATGACTCATCCTTGTTGGTCCAAGCCATGGAGCTCTCTGGTCCAAGGTGA

Coding sequence (CDS)

ATGAAGAGAACTGAGGCTACTGCTTCTTGTCTTGTGGCTTCTTTATTGTTTTGGGCTTCTGCAATAAATGGCTTGCTATCTCCCAAAGGTGTTAACTATGAAGTTCAAGCTCTGATGGGTATAAAAGCTTCACTTCAAGATCCCCATGGCGTGCTTGAGAATTGGGATGGAGACTCTGTTGACCCATGTAGTTGGACTATGGTTACTTGCTCCCCTGACAGCCTTGTCATTGGCCTAGGGACTCCAAGTCAGAACCTGTCTGGGACTCTTTCTTCAAGCATTGGGAATTTGACAAACCTTCAAATTGTGCTGTTGCAGAACAACAATATTACGGGCCCAATCCCCCCAGAGCTTGAGAGGCTCTCAAAGCTCCAAACACTTGATCTATCAAGCAATTTCTTCACAGGGAAAATTCCTTCTTCTTTAGGCCATTTGAGAAGCCTACAGTACTTGAGGCTTAACAACAACAGTCTTTCTGGGGTAATTCCAATGTCTGTAGCTAACATGACACAGCTTGTGTTTCTCGATGTGTCTTATAATAATATCAGTGGCCCTCTCCCCAGATTGCCCAGCAAAACTTTCAACATTGTTGGAAACCCTCTGATCTGTGCAACTGGGTCCGAGGTTGGTTGCCACGGTTCAACTCTAATGCCCATGTCCATGAACTTGAACAGTACACAAACTGGTTTACCTGCTGTGAGATTGAAAAGTCATAAAATGGCCCTTACATTTGGCTTGAGCCTAGCTTGCCTATGTCTGATTGTTCTTGTATTTGGACTGTTCATATGGTGGCGAAGACGAAGCAATCGCCCGACGTTTTTCGATGTAAAAGACGGACAACATGAGGAAATTTCGCTTGGAAATCTGAGAAGGCTCAAGGATGGCAATGCAAGTAGAGGTGATATCCAGTTTCAGACTGAAGTCGAGATGATCAGCCTAGCCGTGCACCGTCATCTCCTCCGACTGTACGGGTTTTGCAACACACCAACCGAAAGGATTCTTGTTTACCCGTATATGTCTAATGGAAGCGTTGCATCACGTCTCAAAGGAAAACCGGTATTAGACTGGGGAACAAGGAAGAGAATTGCTATAGGAGCTTCAAGGGGATTGTTATACCTCCATGAACAATGTGACCCAAAGATCATACATAGAGACGTTAAGGCTGCAAACATATTGCTCGACGACTACTGCGAGGCTGTCGTTGGAGATTTCGGGTTGGCAAAGCTTCTCGACCACCAAGATTCACATGTCACAACTGCTGTGAGAGGCACCGTGGGTCATATAGCTCCTGAGTATCTCTCGACTGGGCAGTCATCCGAGAAAACAGACGTTTTTGGATTCGGAATTCTACTCCTTGAACTGATCACAGGGCAGAAAGCCCTTGAGTTTGGCAAAACAGCTAATCAGAAAGGAGGCATTCTTGAATGGGTGAAGAAGATTCACCAGGAGAAGAAGCTGGAAGTGCTAGTGGACAAGGATCTGAAAGCCAACTATGACAGAGTTGAGCTAGAGGAGATGGTTCAAGTGGCTCTGTTGTGTACACAATACCAACCAGGACACAGACCGAAGATGTCAGAAGTTGTCAGAATGCTTGAAGGAGAAGGTCTTGCTGTCAGATGGGAAGCTTCTCAGAGAGTTGACTCAACAAAGTGCAAACCTCATGATTTCTCTTCATCTGACAGATACTCTGATCTCACAGATGACTCATCCTTGTTGGTCCAAGCCATGGAGCTCTCTGGTCCAAGGTGA

Protein sequence

MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSSSDRYSDLTDDSSLLVQAMELSGPR
Homology
BLAST of Csor.00g312270 vs. ExPASy Swiss-Prot
Match: Q9LFS4 (Protein NSP-INTERACTING KINASE 1 OS=Arabidopsis thaliana OX=3702 GN=NIK1 PE=1 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 3.3e-250
Identity = 444/622 (71.38%), Postives = 504/622 (81.03%), Query Frame = 0

Query: 10  CLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVT 69
           C +  L    S+++GLLSPKGVN+EVQALM IKASL DPHGVL+NWD D+VDPCSWTMVT
Sbjct: 17  CFLGFLCLLCSSVHGLLSPKGVNFEVQALMDIKASLHDPHGVLDNWDRDAVDPCSWTMVT 76

Query: 70  CSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDL 129
           CS ++ VIGLGTPSQNLSGTLS SI NLTNL+IVLLQNNNI G IP E+ RL++L+TLDL
Sbjct: 77  CSSENFVIGLGTPSQNLSGTLSPSITNLTNLRIVLLQNNNIKGKIPAEIGRLTRLETLDL 136

Query: 130 SSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRL 189
           S NFF G+IP S+G+L+SLQYLRLNNNSLSGV P+S++NMTQL FLD+SYNN+SGP+PR 
Sbjct: 137 SDNFFHGEIPFSVGYLQSLQYLRLNNNSLSGVFPLSLSNMTQLAFLDLSYNNLSGPVPRF 196

Query: 190 PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLA 249
            +KTF+IVGNPLIC TG+E  C+G+TL+PMSMNLN T   L A   ++HKMA+  G S+ 
Sbjct: 197 AAKTFSIVGNPLICPTGTEPDCNGTTLIPMSMNLNQTGVPLYAGGSRNHKMAIAVGSSVG 256

Query: 250 CLCLIVLVFGLFIWWRRRSNRPTFFDVKDG-QHEEISLGNLR------------------ 309
            + LI +  GLF+WWR+R N+ TFFDVKDG  HEE+SLGNLR                  
Sbjct: 257 TVSLIFIAVGLFLWWRQRHNQNTFFDVKDGNHHEEVSLGNLRRFGFRELQIATNNFSSKN 316

Query: 310 ------------------------RLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFC 369
                                   RLKDG A  G+IQFQTEVEMISLAVHR+LLRLYGFC
Sbjct: 317 LLGKGGYGNVYKGILGDSTVVAVKRLKDGGALGGEIQFQTEVEMISLAVHRNLLRLYGFC 376

Query: 370 NTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDV 429
            T TE++LVYPYMSNGSVASR+K KPVLDW  RKRIAIGA+RGL+YLHEQCDPKIIHRDV
Sbjct: 377 ITQTEKLLVYPYMSNGSVASRMKAKPVLDWSIRKRIAIGAARGLVYLHEQCDPKIIHRDV 436

Query: 430 KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG 489
           KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG
Sbjct: 437 KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG 496

Query: 490 FGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDL--KANYDRVELE 549
           FGILLLEL+TGQ+A EFGK ANQKG +L+WVKKIHQEKKLE+LVDK+L  K +YD +EL+
Sbjct: 497 FGILLLELVTGQRAFEFGKAANQKGVMLDWVKKIHQEKKLELLVDKELLKKKSYDEIELD 556

Query: 550 EMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDS-TKCKPHD---FSSSD 583
           EMV+VALLCTQY PGHRPKMSEVVRMLEG+GLA +WEASQR DS +KC        SSSD
Sbjct: 557 EMVRVALLCTQYLPGHRPKMSEVVRMLEGDGLAEKWEASQRSDSVSKCSNRINELMSSSD 616

BLAST of Csor.00g312270 vs. ExPASy Swiss-Prot
Match: Q8RY65 (Protein NSP-INTERACTING KINASE 2 OS=Arabidopsis thaliana OX=3702 GN=NIK2 PE=1 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 2.9e-230
Identity = 416/632 (65.82%), Postives = 488/632 (77.22%), Query Frame = 0

Query: 3   RTEATASCLVASLLF------WASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWD 62
           R EA  S  + S  F      + S+ +  L+ KGVN+EV AL+GIK+SL DPHGVL NWD
Sbjct: 5   RREAKKSYALFSSTFFFFFICFLSSSSAELTDKGVNFEVVALIGIKSSLTDPHGVLMNWD 64

Query: 63  GDSVDPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPP 122
             +VDPCSW M+TCS D  VI L  PSQNLSGTLSSSIGNLTNLQ VLLQNN ITG IP 
Sbjct: 65  DTAVDPCSWNMITCS-DGFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPH 124

Query: 123 ELERLSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLD 182
           E+ +L KL+TLDLS+N FTG+IP +L + ++LQYLR+NNNSL+G IP S+ANMTQL FLD
Sbjct: 125 EIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPSSLANMTQLTFLD 184

Query: 183 VSYNNISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLK 242
           +SYNN+SGP+PR  +KTFN++GN  IC TG+E  C+G+   PMS+ LNS+Q        K
Sbjct: 185 LSYNNLSGPVPRSLAKTFNVMGNSQICPTGTEKDCNGTQPKPMSITLNSSQNKSSDGGTK 244

Query: 243 SHKMALTFGLSLACLCLIVLVFGLFIWWRRRSNRPT-FFDVKDGQHEEISLGNLR----- 302
           + K+A+ FG+SL C+CL+++ FG  +WWRRR N+   FFD+ +   EE+ LGNLR     
Sbjct: 245 NRKIAVVFGVSLTCVCLLIIGFGFLLWWRRRHNKQVLFFDINEQNKEEMCLGNLRRFNFK 304

Query: 303 -------------------------------------RLKDGNASRGDIQFQTEVEMISL 362
                                                RLKD N   G++QFQTE+EMISL
Sbjct: 305 ELQSATSNFSSKNLVGKGGFGNVYKGCLHDGSIIAVKRLKDINNGGGEVQFQTELEMISL 364

Query: 363 AVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYL 422
           AVHR+LLRLYGFC T +ER+LVYPYMSNGSVASRLK KPVLDWGTRKRIA+GA RGLLYL
Sbjct: 365 AVHRNLLRLYGFCTTSSERLLVYPYMSNGSVASRLKAKPVLDWGTRKRIALGAGRGLLYL 424

Query: 423 HEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYL 482
           HEQCDPKIIHRDVKAANILLDDY EAVVGDFGLAKLLDH++SHVTTAVRGTVGHIAPEYL
Sbjct: 425 HEQCDPKIIHRDVKAANILLDDYFEAVVGDFGLAKLLDHEESHVTTAVRGTVGHIAPEYL 484

Query: 483 STGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKD 542
           STGQSSEKTDVFGFGILLLELITG +ALEFGK ANQ+G IL+WVKK+ QEKKLE +VDKD
Sbjct: 485 STGQSSEKTDVFGFGILLLELITGLRALEFGKAANQRGAILDWVKKLQQEKKLEQIVDKD 544

Query: 543 LKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEA-SQRVDSTK-- 583
           LK+NYDR+E+EEMVQVALLCTQY P HRPKMSEVVRMLEG+GL  +WEA SQR ++ +  
Sbjct: 545 LKSNYDRIEVEEMVQVALLCTQYLPIHRPKMSEVVRMLEGDGLVEKWEASSQRAETNRSY 604

BLAST of Csor.00g312270 vs. ExPASy Swiss-Prot
Match: Q9FP13 (LRR receptor kinase SERL2 OS=Oryza sativa subsp. japonica OX=39947 GN=SERL2 PE=1 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.2e-218
Identity = 391/618 (63.27%), Postives = 462/618 (74.76%), Query Frame = 0

Query: 25  LLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVTCSPDSLVIGLGTPSQ 84
           LLS KGVN EVQAL+ IK  L+DPHGVL++WD +SVDPCSW M+TCSPD LV GL  PSQ
Sbjct: 23  LLSAKGVNNEVQALIVIKNLLKDPHGVLKSWDQNSVDPCSWAMITCSPDFLVTGLEAPSQ 82

Query: 85  NLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDLSSNFFTGKIPSSLGH 144
           +LSG LS SIGNLTNL+ VLLQNNNITGPIP E+ RL  L+TLDLSSN F G+IPSS+GH
Sbjct: 83  HLSGLLSPSIGNLTNLETVLLQNNNITGPIPAEIGRLENLKTLDLSSNSFYGEIPSSVGH 142

Query: 145 LRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRLPSKTFNIVGNPLICA 204
           L SLQYLRLNNN+LSG  P + AN++ LVFLD+SYNN+SGP+P   ++T+NIVGNPLIC 
Sbjct: 143 LESLQYLRLNNNTLSGPFPSASANLSHLVFLDLSYNNLSGPIPESLARTYNIVGNPLICD 202

Query: 205 TGSEVGCHGSTLMPMSMNLNSTQTGL--PAVRLKSHKMALTFGLSLACLCLIVLVFGLFI 264
              E  C+G+  MPMS +LN ++ G   PA R + HK A+ FG +  C+ L++L  G   
Sbjct: 203 ANREQDCYGTAPMPMSYSLNGSRGGALPPAARDRGHKFAVAFGSTAGCMGLLLLAAGFLF 262

Query: 265 WWRRRSNRPTFFDVKDGQHEEISLGN---------------------------------- 324
           WWR R NR   FDV + Q E ++LGN                                  
Sbjct: 263 WWRHRRNRQILFDVDEQQIENVNLGNVKRFSFRELQAATEGFSGKNILGKGGFGNVYRGQ 322

Query: 325 --------LRRLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMS 384
                   ++RLKDGNA+ G+ QFQTEVEMISLA+HR+LLRLYGFC T TER+LVYP+MS
Sbjct: 323 LPDGTLVAVKRLKDGNAAGGEAQFQTEVEMISLALHRNLLRLYGFCMTATERLLVYPFMS 382

Query: 385 NGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAV 444
           NGSVASRLK KP L+WGTR+RIA+GA+RGL+YLHEQCDPKIIHRDVKAAN+LLD+ CEAV
Sbjct: 383 NGSVASRLKAKPALEWGTRRRIAVGAARGLVYLHEQCDPKIIHRDVKAANVLLDEACEAV 442

Query: 445 VGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKA 504
           VGDFGLAKLLDH++SHVTTAVRGTVGHIAPEYLSTGQSS++TDVFGFGILLLEL+TGQ A
Sbjct: 443 VGDFGLAKLLDHRESHVTTAVRGTVGHIAPEYLSTGQSSDRTDVFGFGILLLELVTGQTA 502

Query: 505 LEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGH 564
           LEFGK++N KG +L+WVKK+  EKK+EVLVDK L   YDRVE+EEMVQVALLCTQY P H
Sbjct: 503 LEFGKSSNHKGAMLDWVKKMQSEKKVEVLVDKGLGGGYDRVEVEEMVQVALLCTQYLPAH 562

Query: 565 RPKMSEVVRMLEGEGLAVRWEA--------------SQRVDSTKCKPHDFSSS--DRYSD 583
           RP+MS+VVRMLEG+GLA RWE               S R         DF+++    +SD
Sbjct: 563 RPRMSDVVRMLEGDGLADRWEKASGHSTAAADSLSHSHRTSDPAPPAADFAAAFGRCFSD 622

BLAST of Csor.00g312270 vs. ExPASy Swiss-Prot
Match: Q93ZS4 (Protein NSP-INTERACTING KINASE 3 OS=Arabidopsis thaliana OX=3702 GN=NIK3 PE=1 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 9.2e-192
Identity = 355/610 (58.20%), Postives = 430/610 (70.49%), Query Frame = 0

Query: 26  LSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVTCSPDSLVIGLGTPSQN 85
           LSP GVNYEV AL+ +K  L DP+ VLENWD +SVDPCSW MV+C+ D  V  L  PSQ+
Sbjct: 27  LSPTGVNYEVTALVAVKNELNDPYKVLENWDVNSVDPCSWRMVSCT-DGYVSSLDLPSQS 86

Query: 86  LSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDLSSNFFTGKIPSSLGHL 145
           LSGTLS  IGNLT LQ V+LQNN ITGPIP  + RL KLQ+LDLS+N FTG+IP+SLG L
Sbjct: 87  LSGTLSPRIGNLTYLQSVVLQNNAITGPIPETIGRLEKLQSLDLSNNSFTGEIPASLGEL 146

Query: 146 RSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRLPSKTFNIVGNPLICAT 205
           ++L YLRLNNNSL G  P S++ +  L  +D+SYNN+SG LP++ ++TF ++GN LIC  
Sbjct: 147 KNLNYLRLNNNSLIGTCPESLSKIEGLTLVDISYNNLSGSLPKVSARTFKVIGNALICGP 206

Query: 206 GSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLACLCLIVLVFGLFIWWR 265
            +   C   + +P  + L          R   H +AL F  S +    +    G+F+WWR
Sbjct: 207 KAVSNC---SAVPEPLTLPQDGPDESGTRTNGHHVALAFAASFSAAFFVFFTSGMFLWWR 266

Query: 266 RRSNRPTFFDVKDGQHEEISLGNL------------------------------------ 325
            R N+  FFDV +    E+SLG+L                                    
Sbjct: 267 YRRNKQIFFDVNEQYDPEVSLGHLKRYTFKELRSATNHFNSKNILGRGGYGIVYKGHLND 326

Query: 326 ------RRLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGS 385
                 +RLKD N + G++QFQTEVE ISLA+HR+LLRL GFC++  ERILVYPYM NGS
Sbjct: 327 GTLVAVKRLKDCNIAGGEVQFQTEVETISLALHRNLLRLRGFCSSNQERILVYPYMPNGS 386

Query: 386 VASRLK----GKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEA 445
           VASRLK    G+P LDW  RK+IA+G +RGL+YLHEQCDPKIIHRDVKAANILLD+  EA
Sbjct: 387 VASRLKDNIRGEPALDWSRRKKIAVGTARGLVYLHEQCDPKIIHRDVKAANILLDEDFEA 446

Query: 446 VVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK 505
           VVGDFGLAKLLDH+DSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK
Sbjct: 447 VVGDFGLAKLLDHRDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK 506

Query: 506 ALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPG 565
           AL+FG++A+QKG +L+WVKK+HQE KL+ L+DKDL   +DRVELEE+VQVALLCTQ+ P 
Sbjct: 507 ALDFGRSAHQKGVMLDWVKKLHQEGKLKQLIDKDLNDKFDRVELEEIVQVALLCTQFNPS 566

Query: 566 HRPKMSEVVRMLEGEGLAVRWEASQ----RVDSTKCKPHDFSSSDR---YSDLTDDSSLL 583
           HRPKMSEV++MLEG+GLA RWEA+Q            P   SSS R   YSD   +SSL+
Sbjct: 567 HRPKMSEVMKMLEGDGLAERWEATQNGTGEHQPPPLPPGMVSSSPRVRYYSDYIQESSLV 626

BLAST of Csor.00g312270 vs. ExPASy Swiss-Prot
Match: Q0WVM4 (Probable LRR receptor-like serine/threonine-protein kinase At2g23950 OS=Arabidopsis thaliana OX=3702 GN=At2g23950 PE=1 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 2.1e-191
Identity = 358/617 (58.02%), Postives = 435/617 (70.50%), Query Frame = 0

Query: 26  LSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVTCSPDSLVIGLGTPSQN 85
           LS +  N EV+AL+ IK  L DPHGV +NWD  SVDPCSWTM++CS D+LVIGLG PSQ+
Sbjct: 26  LSSEPRNPEVEALINIKNELHDPHGVFKNWDEFSVDPCSWTMISCSSDNLVIGLGAPSQS 85

Query: 86  LSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDLSSNFFTGKIPSSLGHL 145
           LSGTLS SIGNLTNL+ V LQNNNI+G IPPE+  L KLQTLDLS+N F+G+IP S+  L
Sbjct: 86  LSGTLSGSIGNLTNLRQVSLQNNNISGKIPPEICSLPKLQTLDLSNNRFSGEIPGSVNQL 145

Query: 146 RSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRLPSKTFNIVGNPLICAT 205
            +LQYLRLNNNSLSG  P S++ +  L FLD+SYNN+ GP+P+ P++TFN+ GNPLIC  
Sbjct: 146 SNLQYLRLNNNSLSGPFPASLSQIPHLSFLDLSYNNLRGPVPKFPARTFNVAGNPLICKN 205

Query: 206 GSEVGCHGS-TLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLACLCLIVLVFGLFIWW 265
                C GS +  P+S++L S+         +++ +A+  G+SL     ++L  G FIW+
Sbjct: 206 SLPEICSGSISASPLSVSLRSSS------GRRTNILAVALGVSLGFAVSVILSLG-FIWY 265

Query: 266 RRRSNRPTFFDVKDGQHEE-ISLGNLR--------------------------------- 325
           R++  R T   + D Q E  + LGNLR                                 
Sbjct: 266 RKKQRRLTMLRISDKQEEGLLGLGNLRSFTFRELHVATDGFSSKSILGAGGFGNVYRGKF 325

Query: 326 ---------RLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSN 385
                    RLKD N + G+ QF+TE+EMISLAVHR+LLRL G+C + +ER+LVYPYMSN
Sbjct: 326 GDGTVVAVKRLKDVNGTSGNSQFRTELEMISLAVHRNLLRLIGYCASSSERLLVYPYMSN 385

Query: 386 GSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVV 445
           GSVASRLK KP LDW TRK+IAIGA+RGL YLHEQCDPKIIHRDVKAANILLD+Y EAVV
Sbjct: 386 GSVASRLKAKPALDWNTRKKIAIGAARGLFYLHEQCDPKIIHRDVKAANILLDEYFEAVV 445

Query: 446 GDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKAL 505
           GDFGLAKLL+H+DSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITG +AL
Sbjct: 446 GDFGLAKLLNHEDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGMRAL 505

Query: 506 EFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGHR 565
           EFGK+ +QKG +LEWV+K+H+E K+E LVD++L   YDR+E+ EM+QVALLCTQ+ P HR
Sbjct: 506 EFGKSVSQKGAMLEWVRKLHKEMKVEELVDRELGTTYDRIEVGEMLQVALLCTQFLPAHR 565

Query: 566 PKMSEVVRMLEGEGLAVRWEASQ----------------RVDSTKCKPHDFSSSDRYSDL 583
           PKMSEVV+MLEG+GLA RW AS                   D      H F SS  + D 
Sbjct: 566 PKMSEVVQMLEGDGLAERWAASHDHSHFYHANMSYRTITSTDGNNQTKHLFGSSG-FEDE 625

BLAST of Csor.00g312270 vs. NCBI nr
Match: KAG6578522.1 (Protein NSP-INTERACTING KINASE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRLKDGNASRG 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRLKDGNASRG
Sbjct: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRLKDGNASRG 300

Query: 301 DIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRK 360
           DIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRK
Sbjct: 301 DIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRK 360

Query: 361 RIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTA 420
           RIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTA
Sbjct: 361 RIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTA 420

Query: 421 VRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKI 480
           VRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKI
Sbjct: 421 VRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKI 480

Query: 481 HQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRW 540
           HQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRW
Sbjct: 481 HQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRW 540

Query: 541 EASQRVDSTKCKPHDFSSSDRYSDLTDDSSLLVQAMELSGPR 582
           EASQRVDSTKCKPHDFSSSDRYSDLTDDSSLLVQAMELSGPR
Sbjct: 541 EASQRVDSTKCKPHDFSSSDRYSDLTDDSSLLVQAMELSGPR 582

BLAST of Csor.00g312270 vs. NCBI nr
Match: XP_022938473.1 (protein NSP-INTERACTING KINASE 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 580/624 (92.95%), Postives = 580/624 (92.95%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 77  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 136

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 137 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 196

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 197 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 256

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 257 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 316

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 317 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 376

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 377 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 436

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 437 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 496

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 497 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 556

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 557 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 616

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 582
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 617 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 676

BLAST of Csor.00g312270 vs. NCBI nr
Match: XP_023550222.1 (protein NSP-INTERACTING KINASE 1-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1141 bits (2951), Expect = 0.0
Identity = 579/624 (92.79%), Postives = 580/624 (92.95%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 76  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 135

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 136 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 195

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 196 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 255

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 256 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 315

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVL+FGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 316 ALTFGLSLACLCLIVLIFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 375

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 376 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 435

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 436 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 495

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 496 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 555

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 556 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 615

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 582
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 616 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 675

BLAST of Csor.00g312270 vs. NCBI nr
Match: XP_022938474.1 (protein NSP-INTERACTING KINASE 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 579/623 (92.94%), Postives = 579/623 (92.94%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 77  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 136

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 137 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 196

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 197 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 256

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 257 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 316

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 317 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 376

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 377 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 436

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 437 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 496

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 497 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 556

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 557 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 616

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 581
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 617 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 676

BLAST of Csor.00g312270 vs. NCBI nr
Match: XP_023550223.1 (protein NSP-INTERACTING KINASE 1-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 578/623 (92.78%), Postives = 579/623 (92.94%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 76  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 135

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 136 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 195

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 196 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 255

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 256 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 315

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVL+FGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 316 ALTFGLSLACLCLIVLIFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 375

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 376 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 435

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 436 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 495

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 496 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 555

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 556 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 615

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 581
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 616 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 675

BLAST of Csor.00g312270 vs. ExPASy TrEMBL
Match: A0A6J1FJ00 (protein NSP-INTERACTING KINASE 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444700 PE=3 SV=1)

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 580/624 (92.95%), Postives = 580/624 (92.95%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 77  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 136

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 137 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 196

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 197 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 256

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 257 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 316

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 317 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 376

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 377 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 436

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 437 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 496

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 497 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 556

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 557 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 616

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 582
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 617 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 676

BLAST of Csor.00g312270 vs. ExPASy TrEMBL
Match: A0A6J1FE68 (protein NSP-INTERACTING KINASE 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444700 PE=3 SV=1)

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 579/623 (92.94%), Postives = 579/623 (92.94%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 77  MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 136

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 137 DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 196

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 197 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 256

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 257 NISGPLPRFPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 316

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 317 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 376

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 377 TNNFSSKNILGKGGFGNVYKGILSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 436

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 437 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 496

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 497 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 556

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 557 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 616

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 581
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 617 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 676

BLAST of Csor.00g312270 vs. ExPASy TrEMBL
Match: A0A6J1JUV2 (protein NSP-INTERACTING KINASE 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489106 PE=3 SV=1)

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 579/624 (92.79%), Postives = 579/624 (92.79%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEV CHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 181 NISGPLPRFPSKTFNIVGNPLICATGSEVSCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 300

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 301 TNNFSSKNILGKGGFGNVYKGLLSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 360

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 481 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 582
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 600

BLAST of Csor.00g312270 vs. ExPASy TrEMBL
Match: A0A6J1JX18 (protein NSP-INTERACTING KINASE 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489106 PE=3 SV=1)

HSP 1 Score: 1137 bits (2941), Expect = 0.0
Identity = 578/623 (92.78%), Postives = 578/623 (92.78%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEV CHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 181 NISGPLPRFPSKTFNIVGNPLICATGSEVSCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 300

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 301 TNNFSSKNILGKGGFGNVYKGLLSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 360

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 481 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 581
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 600

BLAST of Csor.00g312270 vs. ExPASy TrEMBL
Match: A0A6J1JR96 (protein NSP-INTERACTING KINASE 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111489106 PE=3 SV=1)

HSP 1 Score: 1137 bits (2941), Expect = 0.0
Identity = 578/623 (92.78%), Postives = 578/623 (92.78%), Query Frame = 0

Query: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60
           MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV
Sbjct: 1   MKRTEATASCLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSV 60

Query: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120
           DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER
Sbjct: 61  DPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELER 120

Query: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180
           LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN
Sbjct: 121 LSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYN 180

Query: 181 NISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240
           NISGPLPR PSKTFNIVGNPLICATGSEV CHGSTLMPMSMNLNSTQTGLPAVRLKSHKM
Sbjct: 181 NISGPLPRFPSKTFNIVGNPLICATGSEVSCHGSTLMPMSMNLNSTQTGLPAVRLKSHKM 240

Query: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR--------- 300
           ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRR         
Sbjct: 241 ALTFGLSLACLCLIVLVFGLFIWWRRRSNRPTFFDVKDGQHEEISLGNLRRFQFRQLQIA 300

Query: 301 ---------------------------------LKDGNASRGDIQFQTEVEMISLAVHRH 360
                                            LKDGNASRGDIQFQTEVEMISLAVHRH
Sbjct: 301 TNNFSSKNILGKGGFGNVYKGLLSDGTVVAVKRLKDGNASRGDIQFQTEVEMISLAVHRH 360

Query: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420
           LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD
Sbjct: 361 LLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCD 420

Query: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480
           PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS
Sbjct: 421 PKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQS 480

Query: 481 SEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540
           SEKTDVFGFGILLLELITGQKALEFGK ANQKGGILEWVKKIHQEKKLEVLVDKDLKANY
Sbjct: 481 SEKTDVFGFGILLLELITGQKALEFGKAANQKGGILEWVKKIHQEKKLEVLVDKDLKANY 540

Query: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 581
           DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS
Sbjct: 541 DRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDSTKCKPHDFSS 600

BLAST of Csor.00g312270 vs. TAIR 10
Match: AT5G16000.1 (NSP-interacting kinase 1 )

HSP 1 Score: 865.5 bits (2235), Expect = 2.4e-251
Identity = 444/622 (71.38%), Postives = 504/622 (81.03%), Query Frame = 0

Query: 10  CLVASLLFWASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVT 69
           C +  L    S+++GLLSPKGVN+EVQALM IKASL DPHGVL+NWD D+VDPCSWTMVT
Sbjct: 17  CFLGFLCLLCSSVHGLLSPKGVNFEVQALMDIKASLHDPHGVLDNWDRDAVDPCSWTMVT 76

Query: 70  CSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDL 129
           CS ++ VIGLGTPSQNLSGTLS SI NLTNL+IVLLQNNNI G IP E+ RL++L+TLDL
Sbjct: 77  CSSENFVIGLGTPSQNLSGTLSPSITNLTNLRIVLLQNNNIKGKIPAEIGRLTRLETLDL 136

Query: 130 SSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRL 189
           S NFF G+IP S+G+L+SLQYLRLNNNSLSGV P+S++NMTQL FLD+SYNN+SGP+PR 
Sbjct: 137 SDNFFHGEIPFSVGYLQSLQYLRLNNNSLSGVFPLSLSNMTQLAFLDLSYNNLSGPVPRF 196

Query: 190 PSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLA 249
            +KTF+IVGNPLIC TG+E  C+G+TL+PMSMNLN T   L A   ++HKMA+  G S+ 
Sbjct: 197 AAKTFSIVGNPLICPTGTEPDCNGTTLIPMSMNLNQTGVPLYAGGSRNHKMAIAVGSSVG 256

Query: 250 CLCLIVLVFGLFIWWRRRSNRPTFFDVKDG-QHEEISLGNLR------------------ 309
            + LI +  GLF+WWR+R N+ TFFDVKDG  HEE+SLGNLR                  
Sbjct: 257 TVSLIFIAVGLFLWWRQRHNQNTFFDVKDGNHHEEVSLGNLRRFGFRELQIATNNFSSKN 316

Query: 310 ------------------------RLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFC 369
                                   RLKDG A  G+IQFQTEVEMISLAVHR+LLRLYGFC
Sbjct: 317 LLGKGGYGNVYKGILGDSTVVAVKRLKDGGALGGEIQFQTEVEMISLAVHRNLLRLYGFC 376

Query: 370 NTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDV 429
            T TE++LVYPYMSNGSVASR+K KPVLDW  RKRIAIGA+RGL+YLHEQCDPKIIHRDV
Sbjct: 377 ITQTEKLLVYPYMSNGSVASRMKAKPVLDWSIRKRIAIGAARGLVYLHEQCDPKIIHRDV 436

Query: 430 KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG 489
           KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG
Sbjct: 437 KAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFG 496

Query: 490 FGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDL--KANYDRVELE 549
           FGILLLEL+TGQ+A EFGK ANQKG +L+WVKKIHQEKKLE+LVDK+L  K +YD +EL+
Sbjct: 497 FGILLLELVTGQRAFEFGKAANQKGVMLDWVKKIHQEKKLELLVDKELLKKKSYDEIELD 556

Query: 550 EMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEASQRVDS-TKCKPHD---FSSSD 583
           EMV+VALLCTQY PGHRPKMSEVVRMLEG+GLA +WEASQR DS +KC        SSSD
Sbjct: 557 EMVRVALLCTQYLPGHRPKMSEVVRMLEGDGLAEKWEASQRSDSVSKCSNRINELMSSSD 616

BLAST of Csor.00g312270 vs. TAIR 10
Match: AT3G25560.1 (NSP-interacting kinase 2 )

HSP 1 Score: 799.3 bits (2063), Expect = 2.1e-231
Identity = 416/632 (65.82%), Postives = 488/632 (77.22%), Query Frame = 0

Query: 3   RTEATASCLVASLLF------WASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWD 62
           R EA  S  + S  F      + S+ +  L+ KGVN+EV AL+GIK+SL DPHGVL NWD
Sbjct: 5   RREAKKSYALFSSTFFFFFICFLSSSSAELTDKGVNFEVVALIGIKSSLTDPHGVLMNWD 64

Query: 63  GDSVDPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPP 122
             +VDPCSW M+TCS D  VI L  PSQNLSGTLSSSIGNLTNLQ VLLQNN ITG IP 
Sbjct: 65  DTAVDPCSWNMITCS-DGFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPH 124

Query: 123 ELERLSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLD 182
           E+ +L KL+TLDLS+N FTG+IP +L + ++LQYLR+NNNSL+G IP S+ANMTQL FLD
Sbjct: 125 EIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPSSLANMTQLTFLD 184

Query: 183 VSYNNISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLK 242
           +SYNN+SGP+PR  +KTFN++GN  IC TG+E  C+G+   PMS+ LNS+Q        K
Sbjct: 185 LSYNNLSGPVPRSLAKTFNVMGNSQICPTGTEKDCNGTQPKPMSITLNSSQNKSSDGGTK 244

Query: 243 SHKMALTFGLSLACLCLIVLVFGLFIWWRRRSNRPT-FFDVKDGQHEEISLGNLR----- 302
           + K+A+ FG+SL C+CL+++ FG  +WWRRR N+   FFD+ +   EE+ LGNLR     
Sbjct: 245 NRKIAVVFGVSLTCVCLLIIGFGFLLWWRRRHNKQVLFFDINEQNKEEMCLGNLRRFNFK 304

Query: 303 -------------------------------------RLKDGNASRGDIQFQTEVEMISL 362
                                                RLKD N   G++QFQTE+EMISL
Sbjct: 305 ELQSATSNFSSKNLVGKGGFGNVYKGCLHDGSIIAVKRLKDINNGGGEVQFQTELEMISL 364

Query: 363 AVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYL 422
           AVHR+LLRLYGFC T +ER+LVYPYMSNGSVASRLK KPVLDWGTRKRIA+GA RGLLYL
Sbjct: 365 AVHRNLLRLYGFCTTSSERLLVYPYMSNGSVASRLKAKPVLDWGTRKRIALGAGRGLLYL 424

Query: 423 HEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYL 482
           HEQCDPKIIHRDVKAANILLDDY EAVVGDFGLAKLLDH++SHVTTAVRGTVGHIAPEYL
Sbjct: 425 HEQCDPKIIHRDVKAANILLDDYFEAVVGDFGLAKLLDHEESHVTTAVRGTVGHIAPEYL 484

Query: 483 STGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKD 542
           STGQSSEKTDVFGFGILLLELITG +ALEFGK ANQ+G IL+WVKK+ QEKKLE +VDKD
Sbjct: 485 STGQSSEKTDVFGFGILLLELITGLRALEFGKAANQRGAILDWVKKLQQEKKLEQIVDKD 544

Query: 543 LKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEA-SQRVDSTK-- 583
           LK+NYDR+E+EEMVQVALLCTQY P HRPKMSEVVRMLEG+GL  +WEA SQR ++ +  
Sbjct: 545 LKSNYDRIEVEEMVQVALLCTQYLPIHRPKMSEVVRMLEGDGLVEKWEASSQRAETNRSY 604

BLAST of Csor.00g312270 vs. TAIR 10
Match: AT3G25560.3 (NSP-interacting kinase 2 )

HSP 1 Score: 794.3 bits (2050), Expect = 6.7e-230
Identity = 414/630 (65.71%), Postives = 486/630 (77.14%), Query Frame = 0

Query: 3   RTEATASCLVASLLF------WASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWD 62
           R EA  S  + S  F      + S+ +  L+ KGVN+EV AL+GIK+SL DPHGVL NWD
Sbjct: 5   RREAKKSYALFSSTFFFFFICFLSSSSAELTDKGVNFEVVALIGIKSSLTDPHGVLMNWD 64

Query: 63  GDSVDPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPP 122
             +VDPCSW M+TCS D  VI L  PSQNLSGTLSSSIGNLTNLQ VLLQNN ITG IP 
Sbjct: 65  DTAVDPCSWNMITCS-DGFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPH 124

Query: 123 ELERLSKLQTLDLSSNFFTGKIPSSLGHLRSLQYLRLNNNSLSGVIPMSVANMTQLVFLD 182
           E+ +L KL+TLDLS+N FTG+IP +L + ++LQYLR+NNNSL+G IP S+ANMTQL FLD
Sbjct: 125 EIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPSSLANMTQLTFLD 184

Query: 183 VSYNNISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRLK 242
           +SYNN+SGP+PR  +KTFN++GN  IC TG+E  C+G+   PMS+ LNS+Q        K
Sbjct: 185 LSYNNLSGPVPRSLAKTFNVMGNSQICPTGTEKDCNGTQPKPMSITLNSSQNKSSDGGTK 244

Query: 243 SHKMALTFGLSLACLCLIVLVFGLFIWWRRRSNRPT-FFDVKDGQHEEISLGNLR----- 302
           + K+A+ FG+SL C+CL+++ FG  +WWRRR N+   FFD+ +   EE+ LGNLR     
Sbjct: 245 NRKIAVVFGVSLTCVCLLIIGFGFLLWWRRRHNKQVLFFDINEQNKEEMCLGNLRRFNFK 304

Query: 303 -------------------------------------RLKDGNASRGDIQFQTEVEMISL 362
                                                RLKD N   G++QFQTE+EMISL
Sbjct: 305 ELQSATSNFSSKNLVGKGGFGNVYKGCLHDGSIIAVKRLKDINNGGGEVQFQTELEMISL 364

Query: 363 AVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLYL 422
           AVHR+LLRLYGFC T +ER+LVYPYMSNGSVASRLK KPVLDWGTRKRIA+GA RGLLYL
Sbjct: 365 AVHRNLLRLYGFCTTSSERLLVYPYMSNGSVASRLKAKPVLDWGTRKRIALGAGRGLLYL 424

Query: 423 HEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYL 482
           HEQCDPKIIHRDVKAANILLDDY EAVVGDFGLAKLLDH++SHVTTAVRGTVGHIAPEYL
Sbjct: 425 HEQCDPKIIHRDVKAANILLDDYFEAVVGDFGLAKLLDHEESHVTTAVRGTVGHIAPEYL 484

Query: 483 STGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKD 542
           STGQSSEKTDVFGFGILLLELITG +ALEFGK ANQ+G IL+WVKK+ QEKKLE +VDKD
Sbjct: 485 STGQSSEKTDVFGFGILLLELITGLRALEFGKAANQRGAILDWVKKLQQEKKLEQIVDKD 544

Query: 543 LKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEA-SQRVDSTK-- 581
           LK+NYDR+E+EEMVQVALLCTQY P HRPKMSEVVRMLEG+GL  +WEA SQR ++ +  
Sbjct: 545 LKSNYDRIEVEEMVQVALLCTQYLPIHRPKMSEVVRMLEGDGLVEKWEASSQRAETNRSY 604

BLAST of Csor.00g312270 vs. TAIR 10
Match: AT3G25560.2 (NSP-interacting kinase 2 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.5e-229
Identity = 415/633 (65.56%), Postives = 487/633 (76.94%), Query Frame = 0

Query: 3   RTEATASCLVASLLF------WASAINGLLSPKGVNYEVQALMGIKASLQDPHGVLENWD 62
           R EA  S  + S  F      + S+ +  L+ KGVN+EV AL+GIK+SL DPHGVL NWD
Sbjct: 5   RREAKKSYALFSSTFFFFFICFLSSSSAELTDKGVNFEVVALIGIKSSLTDPHGVLMNWD 64

Query: 63  GDSVDPCSWTMVTCSPDSLVIGLGTPSQNLSGTLSSSIGNLTNLQIVLLQNNNITGPIPP 122
             +VDPCSW M+TCS D  VI L  PSQNLSGTLSSSIGNLTNLQ VLLQNN ITG IP 
Sbjct: 65  DTAVDPCSWNMITCS-DGFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPH 124

Query: 123 ELERLSKLQTLDLSSNFFTGKIPSSLGHLRSLQYL-RLNNNSLSGVIPMSVANMTQLVFL 182
           E+ +L KL+TLDLS+N FTG+IP +L + ++LQY  R+NNNSL+G IP S+ANMTQL FL
Sbjct: 125 EIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYFRRVNNNSLTGTIPSSLANMTQLTFL 184

Query: 183 DVSYNNISGPLPRLPSKTFNIVGNPLICATGSEVGCHGSTLMPMSMNLNSTQTGLPAVRL 242
           D+SYNN+SGP+PR  +KTFN++GN  IC TG+E  C+G+   PMS+ LNS+Q        
Sbjct: 185 DLSYNNLSGPVPRSLAKTFNVMGNSQICPTGTEKDCNGTQPKPMSITLNSSQNKSSDGGT 244

Query: 243 KSHKMALTFGLSLACLCLIVLVFGLFIWWRRRSNRPT-FFDVKDGQHEEISLGNLR---- 302
           K+ K+A+ FG+SL C+CL+++ FG  +WWRRR N+   FFD+ +   EE+ LGNLR    
Sbjct: 245 KNRKIAVVFGVSLTCVCLLIIGFGFLLWWRRRHNKQVLFFDINEQNKEEMCLGNLRRFNF 304

Query: 303 --------------------------------------RLKDGNASRGDIQFQTEVEMIS 362
                                                 RLKD N   G++QFQTE+EMIS
Sbjct: 305 KELQSATSNFSSKNLVGKGGFGNVYKGCLHDGSIIAVKRLKDINNGGGEVQFQTELEMIS 364

Query: 363 LAVHRHLLRLYGFCNTPTERILVYPYMSNGSVASRLKGKPVLDWGTRKRIAIGASRGLLY 422
           LAVHR+LLRLYGFC T +ER+LVYPYMSNGSVASRLK KPVLDWGTRKRIA+GA RGLLY
Sbjct: 365 LAVHRNLLRLYGFCTTSSERLLVYPYMSNGSVASRLKAKPVLDWGTRKRIALGAGRGLLY 424

Query: 423 LHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEY 482
           LHEQCDPKIIHRDVKAANILLDDY EAVVGDFGLAKLLDH++SHVTTAVRGTVGHIAPEY
Sbjct: 425 LHEQCDPKIIHRDVKAANILLDDYFEAVVGDFGLAKLLDHEESHVTTAVRGTVGHIAPEY 484

Query: 483 LSTGQSSEKTDVFGFGILLLELITGQKALEFGKTANQKGGILEWVKKIHQEKKLEVLVDK 542
           LSTGQSSEKTDVFGFGILLLELITG +ALEFGK ANQ+G IL+WVKK+ QEKKLE +VDK
Sbjct: 485 LSTGQSSEKTDVFGFGILLLELITGLRALEFGKAANQRGAILDWVKKLQQEKKLEQIVDK 544

Query: 543 DLKANYDRVELEEMVQVALLCTQYQPGHRPKMSEVVRMLEGEGLAVRWEA-SQRVDSTK- 583
           DLK+NYDR+E+EEMVQVALLCTQY P HRPKMSEVVRMLEG+GL  +WEA SQR ++ + 
Sbjct: 545 DLKSNYDRIEVEEMVQVALLCTQYLPIHRPKMSEVVRMLEGDGLVEKWEASSQRAETNRS 604

BLAST of Csor.00g312270 vs. TAIR 10
Match: AT1G60800.1 (NSP-interacting kinase 3 )

HSP 1 Score: 671.4 bits (1731), Expect = 6.6e-193
Identity = 355/610 (58.20%), Postives = 430/610 (70.49%), Query Frame = 0

Query: 26  LSPKGVNYEVQALMGIKASLQDPHGVLENWDGDSVDPCSWTMVTCSPDSLVIGLGTPSQN 85
           LSP GVNYEV AL+ +K  L DP+ VLENWD +SVDPCSW MV+C+ D  V  L  PSQ+
Sbjct: 27  LSPTGVNYEVTALVAVKNELNDPYKVLENWDVNSVDPCSWRMVSCT-DGYVSSLDLPSQS 86

Query: 86  LSGTLSSSIGNLTNLQIVLLQNNNITGPIPPELERLSKLQTLDLSSNFFTGKIPSSLGHL 145
           LSGTLS  IGNLT LQ V+LQNN ITGPIP  + RL KLQ+LDLS+N FTG+IP+SLG L
Sbjct: 87  LSGTLSPRIGNLTYLQSVVLQNNAITGPIPETIGRLEKLQSLDLSNNSFTGEIPASLGEL 146

Query: 146 RSLQYLRLNNNSLSGVIPMSVANMTQLVFLDVSYNNISGPLPRLPSKTFNIVGNPLICAT 205
           ++L YLRLNNNSL G  P S++ +  L  +D+SYNN+SG LP++ ++TF ++GN LIC  
Sbjct: 147 KNLNYLRLNNNSLIGTCPESLSKIEGLTLVDISYNNLSGSLPKVSARTFKVIGNALICGP 206

Query: 206 GSEVGCHGSTLMPMSMNLNSTQTGLPAVRLKSHKMALTFGLSLACLCLIVLVFGLFIWWR 265
            +   C   + +P  + L          R   H +AL F  S +    +    G+F+WWR
Sbjct: 207 KAVSNC---SAVPEPLTLPQDGPDESGTRTNGHHVALAFAASFSAAFFVFFTSGMFLWWR 266

Query: 266 RRSNRPTFFDVKDGQHEEISLGNL------------------------------------ 325
            R N+  FFDV +    E+SLG+L                                    
Sbjct: 267 YRRNKQIFFDVNEQYDPEVSLGHLKRYTFKELRSATNHFNSKNILGRGGYGIVYKGHLND 326

Query: 326 ------RRLKDGNASRGDIQFQTEVEMISLAVHRHLLRLYGFCNTPTERILVYPYMSNGS 385
                 +RLKD N + G++QFQTEVE ISLA+HR+LLRL GFC++  ERILVYPYM NGS
Sbjct: 327 GTLVAVKRLKDCNIAGGEVQFQTEVETISLALHRNLLRLRGFCSSNQERILVYPYMPNGS 386

Query: 386 VASRLK----GKPVLDWGTRKRIAIGASRGLLYLHEQCDPKIIHRDVKAANILLDDYCEA 445
           VASRLK    G+P LDW  RK+IA+G +RGL+YLHEQCDPKIIHRDVKAANILLD+  EA
Sbjct: 387 VASRLKDNIRGEPALDWSRRKKIAVGTARGLVYLHEQCDPKIIHRDVKAANILLDEDFEA 446

Query: 446 VVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK 505
           VVGDFGLAKLLDH+DSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK
Sbjct: 447 VVGDFGLAKLLDHRDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQK 506

Query: 506 ALEFGKTANQKGGILEWVKKIHQEKKLEVLVDKDLKANYDRVELEEMVQVALLCTQYQPG 565
           AL+FG++A+QKG +L+WVKK+HQE KL+ L+DKDL   +DRVELEE+VQVALLCTQ+ P 
Sbjct: 507 ALDFGRSAHQKGVMLDWVKKLHQEGKLKQLIDKDLNDKFDRVELEEIVQVALLCTQFNPS 566

Query: 566 HRPKMSEVVRMLEGEGLAVRWEASQ----RVDSTKCKPHDFSSSDR---YSDLTDDSSLL 583
           HRPKMSEV++MLEG+GLA RWEA+Q            P   SSS R   YSD   +SSL+
Sbjct: 567 HRPKMSEVMKMLEGDGLAERWEATQNGTGEHQPPPLPPGMVSSSPRVRYYSDYIQESSLV 626

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFS43.3e-25071.38Protein NSP-INTERACTING KINASE 1 OS=Arabidopsis thaliana OX=3702 GN=NIK1 PE=1 SV... [more]
Q8RY652.9e-23065.82Protein NSP-INTERACTING KINASE 2 OS=Arabidopsis thaliana OX=3702 GN=NIK2 PE=1 SV... [more]
Q9FP131.2e-21863.27LRR receptor kinase SERL2 OS=Oryza sativa subsp. japonica OX=39947 GN=SERL2 PE=1... [more]
Q93ZS49.2e-19258.20Protein NSP-INTERACTING KINASE 3 OS=Arabidopsis thaliana OX=3702 GN=NIK3 PE=1 SV... [more]
Q0WVM42.1e-19158.02Probable LRR receptor-like serine/threonine-protein kinase At2g23950 OS=Arabidop... [more]
Match NameE-valueIdentityDescription
KAG6578522.10.0100.00Protein NSP-INTERACTING KINASE 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022938473.10.092.95protein NSP-INTERACTING KINASE 1-like isoform X1 [Cucurbita moschata][more]
XP_023550222.10.092.79protein NSP-INTERACTING KINASE 1-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022938474.10.092.94protein NSP-INTERACTING KINASE 1-like isoform X2 [Cucurbita moschata][more]
XP_023550223.10.092.78protein NSP-INTERACTING KINASE 1-like isoform X4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1FJ000.092.95protein NSP-INTERACTING KINASE 1-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1FE680.092.94protein NSP-INTERACTING KINASE 1-like isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JUV20.092.79protein NSP-INTERACTING KINASE 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1JX180.092.78protein NSP-INTERACTING KINASE 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1JR960.092.78protein NSP-INTERACTING KINASE 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT5G16000.12.4e-25171.38NSP-interacting kinase 1 [more]
AT3G25560.12.1e-23165.82NSP-interacting kinase 2 [more]
AT3G25560.36.7e-23065.71NSP-interacting kinase 2 [more]
AT3G25560.21.5e-22965.56NSP-interacting kinase 2 [more]
AT1G60800.16.6e-19358.20NSP-interacting kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 276..531
e-value: 6.9E-13
score: 50.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 298..529
e-value: 1.4E-35
score: 123.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 262..536
score: 28.258825
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 123..182
e-value: 5.1E-10
score: 39.0
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 32..71
e-value: 7.8E-15
score: 54.9
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 31..213
e-value: 3.4E-56
score: 191.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 340..551
e-value: 1.5E-61
score: 209.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 275..339
e-value: 4.8E-11
score: 44.7
NoneNo IPR availablePANTHERPTHR47988:SF6PROTEIN NSP-INTERACTING KINASE 1-LIKE ISOFORM X1coord: 7..292
coord: 290..582
NoneNo IPR availablePANTHERPTHR47988SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1coord: 7..292
coord: 290..582
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 33..205
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 381..393
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 299..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g312270.m01Csor.00g312270.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004672 protein kinase activity