Csor.00g302800 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g302800
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Homocysteine S-methyltransferase family protein
Location	Csor_Chr18: 11400653 .. 11402989 (-)
RNA-Seq Expression	Csor.00g302800
Synteny	Csor.00g302800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGATACAGAACGCGGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCGCTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTTCTCTCTTACCGTCTCTGTTTTTTTTTTTTTTTTCCTCTTCTTTTTGTTTTGTTGATGGTGCATCACGATCTCGTCGGCAATTATTGATTCGATGGCATGTGTAGGTCCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGTTTGTACTGCGAATGGATTGAGCTTTCTTTACTGTTTTATCGACTGTAAACATGAATAGTTTTGTACAATGCACTTTATGACGGCTTTTGCAGTACCTTTTTCTTTAAAACGTTGTACTGTGGTTTGTTTAAACATCGATGATGTTATTTAGTTATTTTAAGGCTAATTAGCAGAATGCATGGGGGGTTCAAAACTAGCATTTCTATCCTGTCGTGAAATGATGAACCAAAATGGAACCGGTATTAGCAGTATTCACTTACTCTTTCTTGTCATGCATTACTAACAATATGTACCAGTATTCACTTACTCTTTCTTGTCATGCATTTTATTCTATTCAGGCCACCATTCCAGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTGAAGAGTAACCCAGGGTATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGGTGACTGACACATTGAATATTGAAATAAGGAGTTTGTAAATGTATGGCTTTCGAACTAAATATATTACCCCCACCCAATCAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGTATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGTTTGTTTGTTGTTAACACTTGTTATATCATCTAAGCTGTGGTTTAATAATACTAGACTCACTCTAATGCCGTCCTGATGATTTCACTGCAACTTATATTCTTATGGTTTGATCTAAGTCATAAAATATCTGAGGAAGCAAATTAAGTATATTTTTAAATCCGTTCGTCTGAATTTACAAGTTGTTCATTTCAAGGGATCAAGACAATTTGACAATCGGCATGGCATCGCCACGCCAATGGGGCTTCCATTGCATGATCTGGTCTCAAATCGTTAACTTCTGAATTTGTTGTCTTCTTTATCATGAATATCTTTTTATAATTTAAATGTGGTTTAAATCCTTTGTTTTAAAACTTATTCCAATTTGCTTATTTTACTTTCAGATATTCTATTTAATTCTCAAACTAGAATAACAACACTTCATTTAGTTGGTCCCGATTGTCACAACATACTAACGTTGAATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTGTCGTGAGCATGTGAGGACATTTGTTTCAATTGGTTATCCAATTTTCATTATTTTCTCTCCTCTTGGTAATCATGTTTCCAACTAAACTTACCTCTATTCCCCCCTCCCTTTCTGATCTAACTCAAAAAATTAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCTGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTAAAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTCACAGCTGTGATTTGATATCGTTTTCGTGTTCACTGGTTTTGACACCATAATATTCCCTTCAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGGTGAGTCAAATGATTTTCCCCACCCCCACTTTGATATTTTCATGGCTTTAGGGCCTACATTGTGATATTTCCAGAAGACTATAACTAATGTAATGCTCTGTTTTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

mRNA sequence

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGATACAGAACGCGGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCGCTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTCCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGCCACCATTCCAGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTGAAGAGTAACCCAGGGTATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGTATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCTGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTAAAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

Coding sequence (CDS)

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGATACAGAACGCGGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCGCTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTCCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGCCACCATTCCAGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTGAAGAGTAACCCAGGGTATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGTATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCTGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTAAAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

Protein sequence

MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGYNYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH

Homology

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BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 7.9e-148
Identity = 244/324 (75.31%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MG++K + LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+KDP+LIK+VH+
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGAD++V+SSYQATIPGFLS+GLS EE E LL++SVKLAVEARD FWD V    G+
Sbjct: 61  EYLEAGADVVVTSSYQATIPGFLSRGLSMEESESLLQKSVKLAVEARDRFWDKVSKTSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSG YG DV +D LK FHRRR+Q+LV ASPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIP+WICF+SVDGENAPSGESF+ECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP F++ LI KF +LT K IVVYPNSGEVWDG+AKKWLPS CFGD +FE  +++WRDL
Sbjct: 241 CAPPQFMDNLIRKFSKLTQKAIVVYPNSGEVWDGKAKKWLPSQCFGDAEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLK 325
           GAK IGGCCRTTPSTI+A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTIKAISRDLK 324

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BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 8.2e-145
Identity = 242/324 (74.69%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M ++K + LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    G+
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE VQIP+WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLK 325
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

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BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.4e-128
Identity = 215/316 (68.04%), Postives = 252/316 (79.75%), Query Frame = 0


Query: 9   LLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGAD 68
           +L+DL+  AGGCAVIDGG ATQLE  GA  ND LWSA CL+  P L+K+VH++YLEAGAD
Sbjct: 3   VLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVKEVHMQYLEAGAD 62

Query: 69  ILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGYNYNRALVA 128
           +++SSSYQATIPGF+++G+S  E E LL  SVKLA EARD FW S        YNRALVA
Sbjct: 63  VIISSSYQATIPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRALVA 122

Query: 129 ASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFETIPNKLEAQ 188
           ASIGSYGAYLADGSEYSG YG D+  + LK FHRRRLQ+L  A PDL+AFE IPN++EAQ
Sbjct: 123 ASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQ 182

Query: 189 ACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGINCTPPHFIE 248
           A VELLEEE VQIPSWICFSSVDG+N  SGESF +CLK +N S+KV  VG+NCTPP FIE
Sbjct: 183 ALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQFIE 242

Query: 249 ALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGC 308
            +IC+F++ T K I VYPNSGEVWDGRAK+WLP  C G   F++L+ RW++ GA  IGGC
Sbjct: 243 GIICEFRKQTKKAIAVYPNSGEVWDGRAKRWLPVECLGHKSFDALAKRWQEAGASLIGGC 302

Query: 309 CRTTPSTIRAVSKVLK 325
           CRTTPSTIRAVSK+LK
Sbjct: 303 CRTTPSTIRAVSKILK 318

[Close]

BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 378.3 bits (970), Expect = 9.1e-104
Identity = 181/331 (54.68%), Postives = 238/331 (71.90%), Query Frame = 0


Query: 4   KKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYL 63
           ++ ++L+ D ++  GG AV+DGG AT+L++HGA  ND LWSA CL+  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWD-SVKSNPGYNY 123
           E+GA+I++++SYQATI GF++KGLS  E E LL RSV++  EAR+ F++   K +  + Y
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 124 ------NRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLL 183
                    LVAAS+GSYGAYLADGSEYSG YG  V  +TLK FHRRR+QIL  +  DL+
Sbjct: 127 AGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLI 186

Query: 184 AFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNA 243
           AFETIPNKLEA+A  +LLEEE++ IP+W  F+S DG + P G+S  EC K  +    V A
Sbjct: 187 AFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVA 246

Query: 244 VGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSR 303
           +GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  S+
Sbjct: 247 IGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSK 306

Query: 304 WRDLGAKFIGGCCRTTPSTIRAVSKVLKESP 328
           WRD GA   GGCCRTTP+TIRA++KVL + P
Sbjct: 307 WRDAGASLFGGCCRTTPNTIRAIAKVLSDEP 337

[Close]

BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.2e-101
Identity = 178/319 (55.80%), Postives = 228/319 (71.47%), Query Frame = 0


Query: 10  LDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADI 69
           + D ++  GG AVIDGGLAT+ E+HGA  ND LWSA CLV  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGYN-----YNR 129
           + S+SYQATI GF +KG S EE E LL++SV++A EAR+S++D   ++   +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++ LK FHRRRLQ+L  +  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGINCTPP 249
           +EAQA  +LLEE +V+IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKF 309
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSTIRAVSKVL 324
           +GGCCRTTP+TIRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

[Close]

BLAST of Csor.00g302800 vs. NCBI nr
Match: KAG6573963.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013023.1 Homocysteine S-methyltransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 674 bits (1739), Expect = 1.03e-244
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. NCBI nr
Match: XP_022945567.1 (homocysteine S-methyltransferase 1 [Cucurbita moschata])

HSP 1 Score: 669 bits (1727), Expect = 6.94e-243
Identity = 325/328 (99.09%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKATTLLDDLIQNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAG+DILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. NCBI nr
Match: XP_023541078.1 (homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667 bits (1720), Expect = 8.10e-242
Identity = 325/328 (99.09%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. NCBI nr
Match: XP_022968494.1 (homocysteine S-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 659 bits (1701), Expect = 6.38e-239
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKA TLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. NCBI nr
Match: XP_022150665.1 (homocysteine S-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 620 bits (1599), Expect = 2.26e-223
Identity = 301/326 (92.33%), Postives = 313/326 (96.01%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M MKKATTLLDDLI+NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+KDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+VSSSYQATIPGFLSKGLS EEGELLLE+SVKLAVEARDSFW+SVKS PG+
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDV+VD LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDG+NAPSGESFEECL+AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES SSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKES 326
           GAKFIGGCCRTTPST RAVSKVLKES
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKES 326

[Close]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL
Match: A0A6J1G1A5 (homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762 PE=4 SV=1)

HSP 1 Score: 669 bits (1727), Expect = 3.36e-243
Identity = 325/328 (99.09%), Postives = 327/328 (99.70%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKATTLLDDLIQNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAG+DILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 3.09e-239
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MGMKKA TLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 620 bits (1599), Expect = 1.09e-223
Identity = 301/326 (92.33%), Postives = 313/326 (96.01%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M MKKATTLLDDLI+NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+KDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+VSSSYQATIPGFLSKGLS EEGELLLE+SVKLAVEARDSFW+SVKS PG+
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDV+VD LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDG+NAPSGESFEECL+AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES SSRWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKES 326
           GAKFIGGCCRTTPST RAVSKVLKES
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKES 326

[Close]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 610 bits (1572), Expect = 1.42e-219
Identity = 296/328 (90.24%), Postives = 310/328 (94.51%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MG++KATTLLDDL+ NAGGCAVIDGGLATQLEKHGAVFND LWSAVCL+ DPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILV+SSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVK  PG+
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRL+ILV ASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFES +SRW  L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESFTSRWCKL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328
           GA FIGGCCRTTPSTIRAVSKVLKES H
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKESSH 328

[Close]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 9.60e-218
Identity = 294/326 (90.18%), Postives = 306/326 (93.87%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           MG+KKATTLLDD + NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+ DP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADILVSSSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVK  PG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQI V ASPDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN+QIPSWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFESLSSRWR+L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESLSSRWRNL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLKES 326
           GA FIGGCCRTTPSTIRAVSKVLKES
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKES 326

[Close]

BLAST of Csor.00g302800 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 514.6 bits (1324), Expect = 5.8e-146
Identity = 242/324 (74.69%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M ++K + LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    G+
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE VQIP+WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLK 325
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of Csor.00g302800 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 473.4 bits (1217), Expect = 1.5e-133
Identity = 228/324 (70.37%), Postives = 265/324 (81.79%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M ++K + LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    G+
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE VQIP+WICF+SVDGE APSGESFEECL+ +NKS+ + A    
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA---- 240

Query: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300
                           LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  +++WRDL
Sbjct: 241 ----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GAKFIGGCCRTTPSTIRAVSKVLK 325
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 304

[Close]

BLAST of Csor.00g302800 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 1.6e-111
Identity = 192/260 (73.85%), Postives = 223/260 (85.77%), Query Frame = 0


Query: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60
           M ++K + LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    G+
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LK FHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE VQIP+WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKELTDK 261
           C PP FIE LI KF + TD+
Sbjct: 241 CAPPQFIENLIRKFAKATDE 260

[Close]

BLAST of Csor.00g302800 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 378.3 bits (970), Expect = 6.5e-105
Identity = 181/331 (54.68%), Postives = 238/331 (71.90%), Query Frame = 0


Query: 4   KKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYL 63
           ++ ++L+ D ++  GG AV+DGG AT+L++HGA  ND LWSA CL+  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWD-SVKSNPGYNY 123
           E+GA+I++++SYQATI GF++KGLS  E E LL RSV++  EAR+ F++   K +  + Y
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 124 ------NRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLL 183
                    LVAAS+GSYGAYLADGSEYSG YG  V  +TLK FHRRR+QIL  +  DL+
Sbjct: 127 AGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLI 186

Query: 184 AFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNA 243
           AFETIPNKLEA+A  +LLEEE++ IP+W  F+S DG + P G+S  EC K  +    V A
Sbjct: 187 AFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVA 246

Query: 244 VGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSR 303
           +GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  S+
Sbjct: 247 IGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSK 306

Query: 304 WRDLGAKFIGGCCRTTPSTIRAVSKVLKESP 328
           WRD GA   GGCCRTTP+TIRA++KVL + P
Sbjct: 307 WRDAGASLFGGCCRTTPNTIRAIAKVLSDEP 337

[Close]

BLAST of Csor.00g302800 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 369.8 bits (948), Expect = 2.3e-102
Identity = 178/319 (55.80%), Postives = 228/319 (71.47%), Query Frame = 0


Query: 10  LDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADI 69
           + D ++  GG AVIDGGLAT+ E+HGA  ND LWSA CLV  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGYN-----YNR 129
           + S+SYQATI GF +KG S EE E LL++SV++A EAR+S++D   ++   +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++ LK FHRRRLQ+L  +  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGINCTPP 249
           +EAQA  +LLEE +V+IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKF 309
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSTIRAVSKVL 324
           +GGCCRTTP+TIRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

The following BLAST results are available for this feature:

BLAST of Csor.00g302800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4ZGQ8	7.9e-148	75.31	Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN...	[more]
Q9SDL7	8.2e-145	74.69	Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1...	[more]
Q9FUN0	2.4e-128	68.04	Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1	[more]
Q8LAX0	9.1e-104	54.68	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ...	[more]
Q9M1W4	3.2e-101	55.80	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1...	[more]

BLAST of Csor.00g302800 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573963.1	1.03e-244	100.00	Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022945567.1	6.94e-243	99.09	homocysteine S-methyltransferase 1 [Cucurbita moschata]	[more]
XP_023541078.1	8.10e-242	99.09	homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968494.1	6.38e-239	98.17	homocysteine S-methyltransferase 1-like [Cucurbita maxima]	[more]
XP_022150665.1	2.26e-223	92.33	homocysteine S-methyltransferase 1 [Momordica charantia]	[more]

BLAST of Csor.00g302800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1A5	3.36e-243	99.09	homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762...	[more]
A0A6J1HZT4	3.09e-239	98.17	homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]
A0A6J1D951	1.09e-223	92.33	homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874...	[more]
A0A1S3BEY1	1.42e-219	90.24	homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ...	[more]
A0A0A0KR42	9.60e-218	90.18	Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660...	[more]

BLAST of Csor.00g302800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25900.1	5.8e-146	74.69	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.3	1.5e-133	70.37	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.2	1.6e-111	73.85	Homocysteine S-methyltransferase family protein	[more]
AT3G22740.1	6.5e-105	54.68	homocysteine S-methyltransferase 3	[more]
AT3G63250.1	2.3e-102	55.80	homocysteine methyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 22..322 e-value: 2.8E-73 score: 247.2
IPR003726	Homocysteine-binding domain	PROSITE	PS50970	HCY	coord: 9..323 score: 48.173496
IPR036589	Homocysteine-binding domain superfamily	GENE3D	3.20.20.330		coord: 10..321 e-value: 2.6E-97 score: 327.9
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 17..325
IPR017226	Betaine-homocysteine S-methyltransferase, BHMT	PIRSF	PIRSF037505	BHMT	coord: 1..259 e-value: 2.1E-8 score: 31.7 coord: 287..327 e-value: 0.097 score: 9.8
None	No IPR available	PIRSR	PIRSR037505-2	PIRSR037505-2	coord: 9..242 e-value: 3.1E-9 score: 34.2
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 1..325
None	No IPR available	PANTHER	PTHR46015:SF7	HOMOCYSTEINE S-METHYLTRANSFERASE 1	coord: 1..325

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g302800.m01	Csor.00g302800.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0033528	S-methylmethionine cycle
molecular_function	GO:0047150	betaine-homocysteine S-methyltransferase activity
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0008270	zinc ion binding