Csor.00g302240 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g302240
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr18: 11093605 .. 11098068 (-)
RNA-Seq ExpressionCsor.00g302240
SyntenyCsor.00g302240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGTGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGTGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGACAACTGTACAGTGTGTCGTTGGGATTGGTCACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATATCGCGGCGGGCGCACGGAACAGAGAGCCACTGAACACGCTGATCAAGGTACGAATTACGCTGTTTGTCTGTTCGTTCAGTTACACGGAACTTTTCATTTGCTTTATTCAATGCATAAAACAAAAATTGGGGGAAAAAAAAAAATATATATATATATATTTTGGTTTAAAAAAAATAAATTAAAATCTAGTCCCTACCATTTTCAAAATTACAGTTTTGTCCCCTTTGATAAAATTTTATAAATAGTTTGAAAACTATATTTATGAAATTTTTAATTTTTAAAATTATATAGACTAAATTTTAATTTTTTTCTAAATCATAGGGATGAAATTTGTAATTTATGGTTATTTTTTTTTTAAATATTACCATATCTATAAGAGGATAATTTTTGGATCAAGAAAGGGTAAAAATGTAATTACACTCGTTGATAAATATGATTTAAGAAGTAATTGTTGAACTAAGAAAAAAGAAAATATTAAATAGAAAAAACATTTAAAATATCTAATAAAATTATAAATTATAAATTTTTTATCTATTTTGTGTTAAAATACGTTTTTTTTTAATTTCGTGTTTAATTGACATTTAAACTTTTAATAATTAGTAAAATAAAATTTAATTACAGAATTAAAAATTCTTAAAAATTTATTAAATATATATTTATAAAAAGGGTATGAAATTCACCTATTATAACACACATTCAATTTCTTTGATTATACACTATGTTTAAAAGAAAATTAGTTGAATTATTATTTTGCCTTTCAAATTTAGTTCTATTTTAACATTTTTTTAATATTCAAATTTAGTTCAAGATCTTAAAATTAGTTCATTATGTTAAATTATTAATAAATTTTCTAAAAAAAATAGTATAGGTTATTCTTATATTTATAAATTTTAGAAATATATTTATCTTCGTAAAATAATAATGTTATTTATCTATATTTTGTAAGTACATACTTTATTTTTTATTTATTTTTAAATGTATTGCTTTAAAAGTGGATCCAACTCCCCCATGACCTTATTATTCTCCCCCCACAAAGGGATATTAATGGGGCAAAAACCAGTTGTTTATATTAAAATATTAATTGTATTAAATATCCTTCAACCATATGAAATTAAATTGAGGGTAGATATTATTTACAGGAGTTAGGAGTTGGGAAGTGGTACATAATTATATAATGTAAAAACTATACATTAATTTTATTTAATAACTTTATCCTGTTTTGGCTTCTTGGCGGAGCTGTAGACTTATCTCTCTTAATACTGTTAATTAACATGTGAAATTAGGGGGAAAAAACAGAGGCCTCCTCGAAGGTACGGAGGTGTTAGAGGATGAAAGTCCCACGTTGGCTAATTTAGGGAATGAGGCTTTTAGGGAAGCCCAAAGCAAAGTCACGAATACCATTATGGAGAGTCATGTTCTCTTCATTTATTTATGTTTTCTCGGTGTCACGAGAGAAATTTAACTTAGTTTAATATTGCTTTATTCTTATCTAGACATTTGGTTATTGAAATTTAAAATGGACATATTTGATCGAGATATTTGAAGTAGTCGATATAATGGAATGATGACATAACCGATCGAATAGGGTGACTAGAAATTGCTTGCACTTATTAAATTTGTTGGCTGATTGTTTAAGAAACTATAGGCTTAATGGGTTAAGTTTGCATCAGGGACTACATAAAATGGGTTCATGCAACCAATCCACAGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACAATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATATGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTTGATGGTTGGACGACGTATAATTCCTCTACATTTAGTAAGTAAATCATAAACTCATAACCCGTCTACTTATTTTTGCTGAATTCTAAGTAGAGATCTTCATGAGGTAGGGCGAAGACAGGGAAGCTGTGAGATCCCATATTAGTTGGGGAGGAGAATGAAACATTCTTTATAAGGGTATAGAAACCTCTACTTAGTAGACGTGTTTTAAAAACCTTGAGGGGAAGTCCGAGAGGGAAAACCCAAAAAGGACCATATTTGCTAGTGGTGAGCTTGAGTTGTTAATAATGGTATAGAGCCAGACACTGGGCGATGTGCCAGCGAGGAGGTGGACACGAGGTGGTGTGCCAGCAAGGACGTTGGGCCCCGAAGTGTGATGGATTGGGGGATCCCACATTGAGTGGAGAATGGAACGAATGCCAGCGAGGACACTGAGCCCTGAAGGGGGTGGATTGTGAGGTCCCACATCAGTTGGGATTACGAATTCTTTATAGGGTGTGAAAACCTCTCCCAAACATACACGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGGAAAAGCCTAAAGAGGACCATATTTGCTAGCGCTGGGCTTGAGCTGTTACTAATGGTATTAGAGCTAGACACCGGGTGATGTGTCAGCGAGGAGGCTGAGCCCCAAAGGGGGTGGATACGAGGCGGTGTGCTAGCAAGGACGTTGGACCCCGAAGGATGGTGGATTAGGAGGTACCACATCGATTAGAGAAGGAAATGAATGCCAGCAAGAAGTCCCGAAGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGAGAACAAAACATTCTTTATAAGGGTGTGGAAACCTCTCCTTGGCAGACGCGTTTTAAAAACTTTGAGGGAAAGCCCGAAAGGGAAAGCCCAAAAAAGACAATATTTGCTAGCGATGAACTTGGGTACTTACATAAGCCTGCTCCGTCTCTATCCTCACTTCCTATTCATACATCATCCTCACAGTGGCGGTCCAAGATAGTTGACGGGCCCAACCTCGTTTAGCTAATAGGAAAAAATTTCTCACCGTTCCCTCCCCATTCCTCGACCCGTTCGGTGCAGTTCCCGTGTATGATGTTAGTTTTACACCGTTTGATATATAAATCTTAGTTTAATGCTTGGATTTTATACGTGTTTATGTGCTTGCAGCTGTTTGTGGGGATAGATTTGTAGCAATAGATGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTAGCACTAAGGAGCAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTCGAGGGCTATCAAGACACTCTCTACGTCCATTCCCTAAGACAATTCTACCGAGAATGCGACATTTACGGCACAGTAGATTTTATATTTGGCAATGCTGCTGCAGTTTTCCAAAAATGCAATCTATACGCCAGAAAGCCACTCCCAAACCAAAAGATCGCCATCACGGCACAGGGCCGAACCGATCCGAACCAAAACACCGGTATCTCGATCCATGACTGCACGATCAAGGCAGCCCCCGACTGGGAGACGGATTACTCAAACTCGACGGCGACAAACTATCTTGGACGGCCGTGGAAGCAGTACTCAAGAACAGTGTATATGCAGTGTTACATTGGGGATTTGATCAGCCCTGTGGGGTGGTTGGAGTGGAATGGAACAGCAGGGTTGGACACTTTGTATTATGGGGAGTTTGAGAATTATGGGCCAGGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAACATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTCTAA

mRNA sequence

ATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGTGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGTGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGACAACTGTACAGTGTGTCGTTGGGATTGGTCACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATATCGCGGCGGGCGCACGGAACAGAGAGCCACTGAACACGCTGATCAAGGGACTACATAAAATGGGTTCATGCAACCAATCCACAGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACAATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATATGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTTGATGGTTGGACGACGTATAATTCCTCTACATTTACTGTTTGTGGGGATAGATTTGTAGCAATAGATGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTAGCACTAAGGAGCAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTCGAGGGCTATCAAGACACTCTCTACGTCCATTCCCTAAGACAATTCTACCGAGAATGCGACATTTACGGCACAGTAGATTTTATATTTGGCAATGCTGCTGCAGTTTTCCAAAAATGCAATCTATACGCCAGAAAGCCACTCCCAAACCAAAAGATCGCCATCACGGCACAGGGCCGAACCGATCCGAACCAAAACACCGGTATCTCGATCCATGACTGCACGATCAAGGCAGCCCCCGACTGGGAGACGGATTACTCAAACTCGACGGCGACAAACTATCTTGGACGGCCGTGGAAGCAGTACTCAAGAACAGTGTATATGCAGTGTTACATTGGGGATTTGATCAGCCCTGTGGGGTGGTTGGAGTGGAATGGAACAGCAGGGTTGGACACTTTGTATTATGGGGAGTTTGAGAATTATGGGCCAGGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAACATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTCTAA

Coding sequence (CDS)

ATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGTGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGTGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGACAACTGTACAGTGTGTCGTTGGGATTGGTCACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATATCGCGGCGGGCGCACGGAACAGAGAGCCACTGAACACGCTGATCAAGGGACTACATAAAATGGGTTCATGCAACCAATCCACAGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACAATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATATGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTTGATGGTTGGACGACGTATAATTCCTCTACATTTACTGTTTGTGGGGATAGATTTGTAGCAATAGATGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTAGCACTAAGGAGCAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTCGAGGGCTATCAAGACACTCTCTACGTCCATTCCCTAAGACAATTCTACCGAGAATGCGACATTTACGGCACAGTAGATTTTATATTTGGCAATGCTGCTGCAGTTTTCCAAAAATGCAATCTATACGCCAGAAAGCCACTCCCAAACCAAAAGATCGCCATCACGGCACAGGGCCGAACCGATCCGAACCAAAACACCGGTATCTCGATCCATGACTGCACGATCAAGGCAGCCCCCGACTGGGAGACGGATTACTCAAACTCGACGGCGACAAACTATCTTGGACGGCCGTGGAAGCAGTACTCAAGAACAGTGTATATGCAGTGTTACATTGGGGATTTGATCAGCCCTGTGGGGTGGTTGGAGTGGAATGGAACAGCAGGGTTGGACACTTTGTATTATGGGGAGTTTGAGAATTATGGGCCAGGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAACATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTCTAA

Protein sequence

MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV
Homology
BLAST of Csor.00g302240 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 4.3e-194
Identity = 339/559 (60.64%), Postives = 418/559 (74.78%), Query Frame = 0

Query: 22  PPPPPP--PPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIK 81
           PP  PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA +
Sbjct: 64  PPSQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASR 123

Query: 82  MSSVIGDYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVE 141
           +S VI  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE
Sbjct: 124 LSKVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVE 183

Query: 142 RVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKR 201
            V S+LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +
Sbjct: 184 HVNSLLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLK 243

Query: 202 RMKGDGGDIAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFI 261
           R K   G I  G  +  REPL TLIKGL K  +C+    C    R L +LG T    I +
Sbjct: 244 RFKASKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILV 303

Query: 262 NNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLI 321
           +  V+V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+
Sbjct: 304 SKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLM 363

Query: 322 GDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSAD 381
           GDGIN+T+ITGNH+VVDGWTTYN S+F V G+RF+A+DVTFRNTAGPEKHQAVALR++A+
Sbjct: 364 GDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAE 423

Query: 382 LSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQK 441
            S+FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QK
Sbjct: 424 GSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQK 483

Query: 442 IAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYI 501
            AITA GR DPNQNTGISI +CTIKAAPD   +    +A  +LGRPWK YSRTV+MQ YI
Sbjct: 484 NAITAHGRLDPNQNTGISIINCTIKAAPDLAAE--PKSAMTFLGRPWKPYSRTVFMQSYI 543

Query: 502 GDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNL 561
            D++ PVGWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N+ +AMNFTVYN 
Sbjct: 544 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 603

Query: 562 TMGDTWLPYTDIPFSGGLV 567
           TMGDTWLP TDIPF GGL+
Sbjct: 604 TMGDTWLPQTDIPFYGGLL 616

BLAST of Csor.00g302240 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.6e-191
Identity = 335/553 (60.58%), Postives = 410/553 (74.14%), Query Frame = 0

Query: 21  SPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIG 80
           SP  P P P    CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 81  DYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSIL 140
            +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   LV+RV S+L
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLL 194

Query: 141 SAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDG 200
             +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R    +R KG  
Sbjct: 195 GGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--NLKRYKGSK 254

Query: 201 GDIAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINNTVVV 260
           G I  G     REPL TLIK L K  +C++   C    R L +LG T    I +   V V
Sbjct: 255 GKIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 261 SSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINR 320
                DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIMLIGDGIN+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 321 TVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYR 380
           T+I+GNHS +DGWTTYNSSTF V GDRFVA+DVTFRNTAGPEKHQAVA+R++AD STFYR
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 381 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQ 440
           CSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQK A+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 441 GRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIGDLISP 500
           GRTDPNQ TGISI +CTI AAPD   D   ST T +LGRPWK YSRTVY+Q YI D++ P
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAAD-PKSTMT-FLGRPWKPYSRTVYIQSYISDVVQP 554

Query: 501 VGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTW 560
           VGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N+ QAMNFTVYN T+GDTW
Sbjct: 555 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 614

Query: 561 LPYTDIPFSGGLV 567
           LP TDIPF GGL+
Sbjct: 615 LPQTDIPFYGGLL 621

BLAST of Csor.00g302240 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 506.1 bits (1302), Expect = 5.0e-142
Identity = 284/581 (48.88%), Postives = 373/581 (64.20%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAF-DSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL+ L L     F  S    P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R +++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K++     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDIAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K       A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + +++ V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAID 360
           V  G YEE VV+ K K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA++
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 360

Query: 361 VTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIF 420
           +TFRNTAGPEKHQAVA+RSSADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIF
Sbjct: 361 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 420

Query: 421 GNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST 480
           GNAA VFQ CNLY R+P+ NQ  AITAQGRTDPNQNTGISIH+CTIK A D  +  SN T
Sbjct: 421 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVS--SNYT 480

Query: 481 ATNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLR 540
              YLGRPWK+YSRTV+MQ YI +++ PVGW EWNG   L TLYY E+ N G G++T+ R
Sbjct: 481 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 540

Query: 541 VDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 567
           V WPGY ++N T A NFTV N  +GD W+  + +P+  GL+
Sbjct: 541 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Csor.00g302240 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 3.1e-136
Identity = 268/558 (48.03%), Postives = 350/558 (62.72%), Query Frame = 0

Query: 30  SADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDS 89
           S+ +CK+T  PK C+S+      S  +   YG FS++  L Q+ K    +  Y+ R  ++
Sbjct: 28  SSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLSQSRKFIRTVDRYIKR--NA 87

Query: 90  RLNQREA-GALEDCRDLSDSNVEF-LRSIESVLEAA-----KSVDEELVERVQSILSAIV 149
            L+Q     AL+DCR L+   +++ L S E+V + +     K +     + +Q++LSA +
Sbjct: 88  HLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKADDIQTLLSAAL 147

Query: 150 TNGQTCIDGLVESRS-----SLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGD 209
           TN QTC++GL  + S     ++   +  P+ +  +L  VSL L T       KKR     
Sbjct: 148 TNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVPKKKKRAGFAW 207

Query: 210 GGDIAAGARNREPLNTLIKGL--HKMGSCNQSTGCSGRKRLLADLGTTG-------IFIN 269
               +  + + +P      G    KM    ++   S  +R LAD  + G       + I+
Sbjct: 208 AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGDGDDGSMVLIS 267

Query: 270 NTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIG 329
           + V VS  G  NFT+I  A+A APNN+    G+F+IYV  G YEEY+ + K K  +M+IG
Sbjct: 268 DIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIG 327

Query: 330 DGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADL 389
           DGIN+TV+TGN SVVDGWTT+NS+TF V    FVA+++TFRNTAGPEKHQAVALRS AD 
Sbjct: 328 DGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADF 387

Query: 390 STFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKI 449
           S FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ CNLY RKP+PNQ  
Sbjct: 388 SIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFN 447

Query: 450 AITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIG 509
           AITAQGR+DPNQNTG SI +CTIK A D  +  SN T   YLGRPWK+YSRTVYMQ YI 
Sbjct: 448 AITAQGRSDPNQNTGTSIQNCTIKPADDLVS--SNYTVKTYLGRPWKEYSRTVYMQSYID 507

Query: 510 DLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLT 567
             + PVGW EWNG   L TLYY E+ N GPG+NT+ RV WPGY ++N T A NFTV  L 
Sbjct: 508 GFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLF 567

BLAST of Csor.00g302240 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 478.8 bits (1231), Expect = 8.6e-134
Identity = 259/544 (47.61%), Postives = 334/544 (61.40%), Query Frame = 0

Query: 31  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSR 90
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR     
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDR-KGKV 95

Query: 91  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCID 150
             +   GALEDC+ L+   +++L S     ++ K++     E V + LSA +TN QTC++
Sbjct: 96  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 151 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDIAA 210
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 211 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINNTVVVSSTGADNFT 270
           G RN + PL        KM    ++   +  +R L       + +++ V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 271 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSV 330
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M+IGDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 335

Query: 331 VDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDT 390
           VDGWTT+NS+TF + G  F+ +++T RNTAGP K QAVALRS  DLS FY CSFE YQDT
Sbjct: 336 VDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDT 395

Query: 391 LYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNT 450
           LY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q   +TAQGRTDPNQNT
Sbjct: 396 LYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNT 455

Query: 451 GISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGT 510
           G +IH CTI+ A D  T  SN T   YLGRPWK+YSRTV MQ YI   + P GW  W+G 
Sbjct: 456 GTAIHGCTIRPADDLAT--SNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 515

Query: 511 AGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFS 567
             L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV N  +G+ W+  T +PF 
Sbjct: 516 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559

BLAST of Csor.00g302240 vs. NCBI nr
Match: KAG6573907.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1145 bits (2963), Expect = 0.0
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566

BLAST of Csor.00g302240 vs. NCBI nr
Match: XP_022945508.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata] >XP_022945509.1 probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata])

HSP 1 Score: 1135 bits (2935), Expect = 0.0
Identity = 562/566 (99.29%), Postives = 564/566 (99.65%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of Csor.00g302240 vs. NCBI nr
Match: XP_023541032.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 560/566 (98.94%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIML+GDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLI+PVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLINPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of Csor.00g302240 vs. NCBI nr
Match: XP_022968171.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 558/566 (98.59%), Postives = 560/566 (98.94%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPP  SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPP--SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of Csor.00g302240 vs. NCBI nr
Match: XP_038891461.1 (probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida])

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 488/570 (85.61%), Postives = 519/570 (91.05%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           MNSV  IFL LL L  AGA+DSPPP     S  LCKSTLYPKLCRSILS+IRFSPSDPYG
Sbjct: 1   MNSVPFIFLFLLFLGFAGAYDSPPP-----STALCKSTLYPKLCRSILSTIRFSPSDPYG 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESV 120
           YGKFSVK CLKQAIKMSSVIG YLDRG DSRLN+REAGAL DC++LSDSNVEFLRSIE V
Sbjct: 61  YGKFSVKQCLKQAIKMSSVIGHYLDRGRDSRLNRREAGALSDCKELSDSNVEFLRSIEGV 120

Query: 121 LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLG 180
           L+AA+ VDEE VERV+SILSAIVTNGQTCIDGLVES SS GN L+AP+S AG+LYSVSLG
Sbjct: 121 LDAAEGVDEERVERVESILSAIVTNGQTCIDGLVESGSSFGNVLSAPLSIAGELYSVSLG 180

Query: 181 LVTNSLSRLWKKRRMKG--DGGDIAAGARNREPLNTLIKGLHKMGSCN-QSTGCSGRKRL 240
           LV+NSLSR WKKRR KG  DGG+  AG RNREPLNTLIKGLHKMGSCN QS  CSGRKRL
Sbjct: 181 LVSNSLSRRWKKRRQKGNGDGGNFPAGPRNREPLNTLIKGLHKMGSCNNQSRKCSGRKRL 240

Query: 241 LADLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVV 300
           L +LG+TGI INNTVVVSSTG DNFTSIGDAIAFAPNNSMPQDGYFVIYVR GYYEEYVV
Sbjct: 241 LTNLGSTGILINNTVVVSSTGGDNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVV 300

Query: 301 VPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEK 360
           +PK+KTN+MLIGDGINRTVITGN +V+DGWTTYNSSTFTVCGD FVAID+TFRNTAGPEK
Sbjct: 301 IPKFKTNVMLIGDGINRTVITGNRNVIDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEK 360

Query: 361 HQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCN 420
           HQAVA+R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CN
Sbjct: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCN 420

Query: 421 LYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQ 480
           LYARKPLPNQK A TAQGRTDPNQNTGISIH+CTI AAPDW  D  ++T TNYLGRPWKQ
Sbjct: 421 LYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIMAAPDWVMD--SNTTTNYLGRPWKQ 480

Query: 481 YSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNI 540
           YSRTVYMQ YIGDLISPVGWLEWNGT GL+TLYYGEFENYGPGANTSLRV+WPGYSLMN+
Sbjct: 481 YSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNL 540

Query: 541 TQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 566
           TQAMNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 TQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Csor.00g302240 vs. ExPASy TrEMBL
Match: A0A6J1G137 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1)

HSP 1 Score: 1135 bits (2935), Expect = 0.0
Identity = 562/566 (99.29%), Postives = 564/566 (99.65%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of Csor.00g302240 vs. ExPASy TrEMBL
Match: A0A6J1HU46 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1)

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 558/566 (98.59%), Postives = 560/566 (98.94%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPP  SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPP--SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of Csor.00g302240 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 487/569 (85.59%), Postives = 520/569 (91.39%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           MNS   IFL LLSLR AGA DSPPP     SA LCKSTLYPKLCRSILSSIRFSPSDPYG
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPP-----SAVLCKSTLYPKLCRSILSSIRFSPSDPYG 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESV 120
           YGKFSV+ CLKQAIKMSSVIG+YLDRG DSRLN++E GALEDCR+LSDSNVEFL+SI+ V
Sbjct: 61  YGKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGV 120

Query: 121 LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLG 180
           LEAA+ VDEEL+ER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLG
Sbjct: 121 LEAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLG 180

Query: 181 LVTNSLSRLWKKRRMKGD--GGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLL 240
           LV NSLSR WKKR  KGD  GG+  A A++REPLNTLIKGLH MGSCNQST CSGRKR+L
Sbjct: 181 LVKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRML 240

Query: 241 ADLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVV 300
            DLGTTGI INNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR GYY+EYVVV
Sbjct: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVV 300

Query: 301 PKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKH 360
           PKYKTNIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GD FVAID+TFRNTAG EKH
Sbjct: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360

Query: 361 QAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNL 420
           QAVA+R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ+CNL
Sbjct: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420

Query: 421 YARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQY 480
           YARKPLPNQK A TAQGRTDPNQ+TGISIH+CTI AAP+W  D  N T TNYLGRPWK +
Sbjct: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDL-NETTTNYLGRPWKPF 480

Query: 481 SRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNIT 540
           SRTVYMQ YIGDLISPVGWLEWNGT GLDTLYYGEFENYGPGANTSLRVDW G+SLMNIT
Sbjct: 481 SRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNIT 540

Query: 541 QAMNFTVYNLTMGDTWLPYTDIPFSGGLV 566
           +AMNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 EAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Csor.00g302240 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 489/569 (85.94%), Postives = 520/569 (91.39%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           MNS+  IFLLLLSL  AGA  SPP      S  LCKSTLYPKLCRSILSSIRFSPSDPYG
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPS-----STALCKSTLYPKLCRSILSSIRFSPSDPYG 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESV 120
           YGKFSVK C+KQAIKMS+VIGDYLDRG DSRLN+REAGAL DCR+LSD NVEFLRSIESV
Sbjct: 61  YGKFSVKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESV 120

Query: 121 LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLG 180
           LEAA+ VDEELVERV+S LSAIVTNGQTC+DGLVESRSSLG AL+AP+ SAG+LYSVSLG
Sbjct: 121 LEAAEGVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLG 180

Query: 181 LVTNSLSRLWKKRRMKGDG-GDIAAGARNREPLNTLIKGLHKMGSCNQ-STGCSGRKRLL 240
           LV+N++SR WKKRR KG G G +  G R+RE LNTLIKGLHKM  CN  ST CSGRKRLL
Sbjct: 181 LVSNAMSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLL 240

Query: 241 ADLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVV 300
            DLG++GI INNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVR GYYEEYVVV
Sbjct: 241 TDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVV 300

Query: 301 PKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKH 360
           PK+KTNIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKH
Sbjct: 301 PKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKH 360

Query: 361 QAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNL 420
           QAVALR+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNL
Sbjct: 361 QAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNL 420

Query: 421 YARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQY 480
           YARKPLPNQK A TAQGRTDPNQNTGISIH+CTIKAAPDW  D SN+T TNYLGRPWKQY
Sbjct: 421 YARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMD-SNTTTTNYLGRPWKQY 480

Query: 481 SRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNIT 540
           SRTVYMQ YIGDLISPVGWLEWNGT GL+TLYYGE+ENYGPGANTSLRV+WPG+SLMN+T
Sbjct: 481 SRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLT 540

Query: 541 QAMNFTVYNLTMGDTWLPYTDIPFSGGLV 566
           QAMNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 QAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Csor.00g302240 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 489/567 (86.24%), Postives = 519/567 (91.53%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           MNS+  IFLLLLSLR AGA DSPPP     SA LCK+TLYPKLC SILSSIRFSPSDPYG
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPP-----SAALCKTTLYPKLCHSILSSIRFSPSDPYG 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESV 120
           YGKFSVK CLKQAIKMSSVIG YL RG  SRLNQREAGAL+DCR+L+D NVEFLRSIESV
Sbjct: 61  YGKFSVKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESV 120

Query: 121 LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLG 180
           LEAA+ VDE++VERV+SILSAIVTNGQTCIDGLVES+SSLG+AL+AP+S+AG+LYSVSLG
Sbjct: 121 LEAAEGVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLG 180

Query: 181 LVTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLAD 240
           LV NSLSR WKKRR    GG       NREPL TLIKGL KMGSCNQST CSGR+RLL D
Sbjct: 181 LVKNSLSRRWKKRRQSFPGG-----GGNREPLITLIKGLRKMGSCNQSTICSGRERLLTD 240

Query: 241 LGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPK 300
           LGTTGIFINNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVR GYYEEYVVVPK
Sbjct: 241 LGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPK 300

Query: 301 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQA 360
           YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQA
Sbjct: 301 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQA 360

Query: 361 VALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYA 420
           VA+R+SADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQ+CNLYA
Sbjct: 361 VAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYA 420

Query: 421 RKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSR 480
           RKPLPNQK A TAQGRTDPNQ+TGISIH+CTI+AAPDW  D SN+T  NYLGRPWKQYSR
Sbjct: 421 RKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMD-SNATIANYLGRPWKQYSR 480

Query: 481 TVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQA 540
           TVYMQ YIGDLISPVGWLEWNGTAGL+TLYYGEFEN+GPGANTS+RV+WPGYSLMN TQA
Sbjct: 481 TVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQA 540

Query: 541 MNFTVYNLTMGDTWLPYTDIPFSGGLV 566
           MNFTVYN TMGDTWLPYTDIPF+GGLV
Sbjct: 541 MNFTVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of Csor.00g302240 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 679.1 bits (1751), Expect = 3.1e-195
Identity = 339/559 (60.64%), Postives = 418/559 (74.78%), Query Frame = 0

Query: 22  PPPPPP--PPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIK 81
           PP  PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA +
Sbjct: 64  PPSQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASR 123

Query: 82  MSSVIGDYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVE 141
           +S VI  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE
Sbjct: 124 LSKVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVE 183

Query: 142 RVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKR 201
            V S+LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +
Sbjct: 184 HVNSLLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLK 243

Query: 202 RMKGDGGDIAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFI 261
           R K   G I  G  +  REPL TLIKGL K  +C+    C    R L +LG T    I +
Sbjct: 244 RFKASKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILV 303

Query: 262 NNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLI 321
           +  V+V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+
Sbjct: 304 SKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLM 363

Query: 322 GDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSAD 381
           GDGIN+T+ITGNH+VVDGWTTYN S+F V G+RF+A+DVTFRNTAGPEKHQAVALR++A+
Sbjct: 364 GDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAE 423

Query: 382 LSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQK 441
            S+FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QK
Sbjct: 424 GSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQK 483

Query: 442 IAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYI 501
            AITA GR DPNQNTGISI +CTIKAAPD   +    +A  +LGRPWK YSRTV+MQ YI
Sbjct: 484 NAITAHGRLDPNQNTGISIINCTIKAAPDLAAE--PKSAMTFLGRPWKPYSRTVFMQSYI 543

Query: 502 GDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNL 561
            D++ PVGWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N+ +AMNFTVYN 
Sbjct: 544 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 603

Query: 562 TMGDTWLPYTDIPFSGGLV 567
           TMGDTWLP TDIPF GGL+
Sbjct: 604 TMGDTWLPQTDIPFYGGLL 616

BLAST of Csor.00g302240 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 669.8 bits (1727), Expect = 1.9e-192
Identity = 335/553 (60.58%), Postives = 410/553 (74.14%), Query Frame = 0

Query: 21  SPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIG 80
           SP  P P P    CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 81  DYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSIL 140
            +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   LV+RV S+L
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLL 194

Query: 141 SAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDG 200
             +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R    +R KG  
Sbjct: 195 GGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--NLKRYKGSK 254

Query: 201 GDIAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINNTVVV 260
           G I  G     REPL TLIK L K  +C++   C    R L +LG T    I +   V V
Sbjct: 255 GKIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 261 SSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINR 320
                DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIMLIGDGIN+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 321 TVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYR 380
           T+I+GNHS +DGWTTYNSSTF V GDRFVA+DVTFRNTAGPEKHQAVA+R++AD STFYR
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 381 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQ 440
           CSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQK A+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 441 GRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIGDLISP 500
           GRTDPNQ TGISI +CTI AAPD   D   ST T +LGRPWK YSRTVY+Q YI D++ P
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAAD-PKSTMT-FLGRPWKPYSRTVYIQSYISDVVQP 554

Query: 501 VGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTW 560
           VGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N+ QAMNFTVYN T+GDTW
Sbjct: 555 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 614

Query: 561 LPYTDIPFSGGLV 567
           LP TDIPF GGL+
Sbjct: 615 LPQTDIPFYGGLL 621

BLAST of Csor.00g302240 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 506.1 bits (1302), Expect = 3.6e-143
Identity = 284/581 (48.88%), Postives = 373/581 (64.20%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAF-DSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL+ L L     F  S    P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R +++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K++     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDIAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K       A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + +++ V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAID 360
           V  G YEE VV+ K K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA++
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 360

Query: 361 VTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIF 420
           +TFRNTAGPEKHQAVA+RSSADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIF
Sbjct: 361 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 420

Query: 421 GNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST 480
           GNAA VFQ CNLY R+P+ NQ  AITAQGRTDPNQNTGISIH+CTIK A D  +  SN T
Sbjct: 421 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVS--SNYT 480

Query: 481 ATNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLR 540
              YLGRPWK+YSRTV+MQ YI +++ PVGW EWNG   L TLYY E+ N G G++T+ R
Sbjct: 481 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 540

Query: 541 VDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 567
           V WPGY ++N T A NFTV N  +GD W+  + +P+  GL+
Sbjct: 541 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Csor.00g302240 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 486.9 bits (1252), Expect = 2.2e-137
Identity = 268/558 (48.03%), Postives = 350/558 (62.72%), Query Frame = 0

Query: 30  SADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDS 89
           S+ +CK+T  PK C+S+      S  +   YG FS++  L Q+ K    +  Y+ R  ++
Sbjct: 28  SSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLSQSRKFIRTVDRYIKR--NA 87

Query: 90  RLNQREA-GALEDCRDLSDSNVEF-LRSIESVLEAA-----KSVDEELVERVQSILSAIV 149
            L+Q     AL+DCR L+   +++ L S E+V + +     K +     + +Q++LSA +
Sbjct: 88  HLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKADDIQTLLSAAL 147

Query: 150 TNGQTCIDGLVESRS-----SLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGD 209
           TN QTC++GL  + S     ++   +  P+ +  +L  VSL L T       KKR     
Sbjct: 148 TNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVPKKKKRAGFAW 207

Query: 210 GGDIAAGARNREPLNTLIKGL--HKMGSCNQSTGCSGRKRLLADLGTTG-------IFIN 269
               +  + + +P      G    KM    ++   S  +R LAD  + G       + I+
Sbjct: 208 AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGDGDDGSMVLIS 267

Query: 270 NTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIG 329
           + V VS  G  NFT+I  A+A APNN+    G+F+IYV  G YEEY+ + K K  +M+IG
Sbjct: 268 DIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIG 327

Query: 330 DGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADL 389
           DGIN+TV+TGN SVVDGWTT+NS+TF V    FVA+++TFRNTAGPEKHQAVALRS AD 
Sbjct: 328 DGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADF 387

Query: 390 STFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKI 449
           S FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ CNLY RKP+PNQ  
Sbjct: 388 SIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFN 447

Query: 450 AITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIG 509
           AITAQGR+DPNQNTG SI +CTIK A D  +  SN T   YLGRPWK+YSRTVYMQ YI 
Sbjct: 448 AITAQGRSDPNQNTGTSIQNCTIKPADDLVS--SNYTVKTYLGRPWKEYSRTVYMQSYID 507

Query: 510 DLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLT 567
             + PVGW EWNG   L TLYY E+ N GPG+NT+ RV WPGY ++N T A NFTV  L 
Sbjct: 508 GFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLF 567

BLAST of Csor.00g302240 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 478.8 bits (1231), Expect = 6.1e-135
Identity = 259/544 (47.61%), Postives = 334/544 (61.40%), Query Frame = 0

Query: 31  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSR 90
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR     
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDR-KGKV 95

Query: 91  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCID 150
             +   GALEDC+ L+   +++L S     ++ K++     E V + LSA +TN QTC++
Sbjct: 96  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 151 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDIAA 210
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 211 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINNTVVVSSTGADNFT 270
           G RN + PL        KM    ++   +  +R L       + +++ V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 271 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSV 330
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M+IGDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 335

Query: 331 VDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDT 390
           VDGWTT+NS+TF + G  F+ +++T RNTAGP K QAVALRS  DLS FY CSFE YQDT
Sbjct: 336 VDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDT 395

Query: 391 LYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNT 450
           LY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q   +TAQGRTDPNQNT
Sbjct: 396 LYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNT 455

Query: 451 GISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGT 510
           G +IH CTI+ A D  T  SN T   YLGRPWK+YSRTV MQ YI   + P GW  W+G 
Sbjct: 456 GTAIHGCTIRPADDLAT--SNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 515

Query: 511 AGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFS 567
             L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV N  +G+ W+  T +PF 
Sbjct: 516 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CB14.3e-19460.64Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF772.6e-19160.58Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK75.0e-14248.88Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX43.1e-13648.03Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O222568.6e-13447.61Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
KAG6573907.10.0100.00putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp... [more]
XP_022945508.10.099.29probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata] >XP_022... [more]
XP_023541032.10.098.94probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita pepo subsp. pepo][more]
XP_022968171.10.098.59probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima][more]
XP_038891461.10.085.61probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G1370.099.29Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1[more]
A0A6J1HU460.098.59Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1[more]
A0A6J1INW00.085.59Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1[more]
A0A1S3BFR90.085.94Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1[more]
A0A6J1D8L40.086.24Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.23.1e-19560.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.11.9e-19260.58Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.13.6e-14348.88Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.12.2e-13748.03Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.16.1e-13547.61Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 25..180
e-value: 3.0E-23
score: 93.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 32..179
e-value: 4.2E-20
score: 72.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 31..181
e-value: 5.5E-10
score: 36.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 251..550
e-value: 8.3E-135
score: 448.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 31..184
e-value: 7.0E-25
score: 89.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 32..183
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 245..565
e-value: 3.1E-114
score: 383.6
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 22..565
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 22..565
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 33..183
e-value: 6.0053E-30
score: 113.302
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 397..406
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 246..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g302240.m01Csor.00g302240.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity