Csor.00g300950 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g300950
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type domain-containing protein
LocationCsor_Chr18: 10167106 .. 10170698 (+)
RNA-Seq ExpressionCsor.00g300950
SyntenyCsor.00g300950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCCTGTTTGTCCCTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGCCGGAATCTGCTTGTCCTTTTGCGAAATCCGGCCGTTCCGATGATGCTTCTTCACGAAAACAGCAATCATCGGAGTCTTCTTGTCCGTTTTCGAAATCTGCTCGTTCTGACGACGGTTCTTTGAAGAAGGATCAGCCTGAGGCGGAGAAAGACGTTGCCGATGCTGCTAAAACTGGCGGCAAATGTCCGTTCGGATATGATTCTCAAACTTTCAAGATTGGTCCTCTAAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAGTAATTTATCAGCTATGGTGCTTTTTGATCTTCGTTTTAGTTTGTTTTGGGAATCCTTCAGGCATTTATTCGTTTGTTTCTTGTTGAATTGAACTAGAGCATGTATATCACGCTTTAATGATTGCCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCACACGCTGATCTGCAAGCCACGGTCGATCGATTCATTGAAGGTCATGCGAGAATCAAGAGATCTCAGATGAACTCAGACAAAGAGCAGGAGGAAGTTAGTGAAAGTAAAGTGGTTATTTATGAAGATTTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGTAATATTCCTTGTTGTTTTCTTCATGTTTCGTTCATGCCCTTGATCTCTGATTGCATGTTTGATCATCTCACTACAATCAATTGAATCTTTCCTTTGATGAAATCCGTTTGTTTGGAGGATCTAGTTAGTTTTTGTAAACATCCAAGTCTACATATTGTCTTCTTTAGGCTTTCCCTTTTGGGTTTCCCCTCAAGGTTTTTAAAACGTGTCTGCTGGGAGAGGTTTCAACACCTTATAAAGAATGTTTCATTCTCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCATTCGGGGTCCAACATCTTCATTGGCACTCATTTCTCTTTCCAATTGATGTGGGATCTCACGATCCACCCCTTTTTGGGTCCTAACGTCCTCGTTAGCACATCGCCCGGTGTCTTACTCTAATATCATTTGTAACAGCTCAAGCCCATTGCTAGCAAATATTGTCCTCTTTGGGCTTCCACTTTCAGGCTTCCCTCAAAGGTTTTTAAATCGCGTCTACTAGGGAGAGGTTTCTATACCTTTATAAAGAATGTTTTGTTCTCCTCTCCAACCGATGTGAGATCGAGAACACACAGTAAGAAAGCAAGAATTTAGGAAACGGTTAACTAAACACCATTTACAGAATTGGGAAACTCCATTTCCTAACATCCCTTTTTGTTCTTAAAGGTTTTGAACTCAACTCTGGCTTTTATTGCAGGCATTTCGTGCCCAGAATATTGAAAGTGCCAAATCCAGGCTCACTATCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAGCGGGCAATTCATCAGAATTATGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGGTATGTTGGCTCCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATGAATATCGTCCAGTAGTTTAGTGTTTATTGAAACTTAGGTGGTGAGTTTTTGAATTAGATGTATCTCTTTGAGTCCCCTCGGTTTCACATCGGCCTATTTGTTGGTAACAATGGTCAACTAATAGGTCTTTTAGGCATTCAGATTCTGAGTTATCATTCTAACTACTAGCATGTGTAATTAAATGAAATCCTGATGTAAATATTCCTTGCAAGTATCTGAACAGTATTATGTGTCCAAATTTTTTTAGTTCACCAAAACAGTATCCATAAATCTTGATGTAAAGGTCTGTTGTTCGATCCTACTTTACAGTCGAGCAGCTGGGGATGCTAGTTCTGCAATAAAGCACTTCGAAGAAAGTGTAGAGTTTCTCTCAAAACTACCCGAAAAGACTCGCGAGGTTCTTTATTAGAAACTTTTGTAAGTTATCAACTGCTTGCTTTTGTAATACTTAGTAACCCTTTTCTTCTTAATTCAGGTCACGCATACACTTTCTGTTTCACTAAATAAAATTGGCGATCTTAAATATTATGAAGGAGACCTTCAAGCTGCAAGATCTTATTATCAGCAGTCTCTTAATGTTCGCCAAGATGCAAGCAAGCATCATTCAGAAGATCCATCCCAGGTTTGTGATTGTGAATTTCTTATATGAAATTTTTTATCTTAAAAGATAACATGTCGTGTATGCTATCCAGTTCTAGAACCTTTTAGTGATAAGTAGTTTCGTTGAAGGTCCATCGACCAAAATGGATGTTAATAGAGACAACCCTTTACCTTAAGCCTGACAAATCTCGTTTCACTTGCCCTACCGCTTTCATTGCCCGTAAAGACTCGAGATCTACAGCCCTAGCGAGCAAAAACTAATTCTTGAACATCATCAACTGCATCTTATACACTATGAAATAAAAACTTAAAGGAATCTTTTTGGGCGGACTAGCAAGAAGCTCAAGGGTACAAAAAACTGACCTTAATTCCTCATTCCCTTTCAAAACTGTCCAGTGTTTTCTTTTGCATTCCGTGTACCCGAAAGGAACGCAAAGTTATACCATTAAATACCGACATACTTTTGCAAATCAACTTGATGTAGTAGCTTAGTAGGGATAGTTCGAAAATTTTTGCATCTTTTTTGAAGTTTACTGTTTTAGTTATTGACTGTTAGCTTAGTAGGGACCTTGAAATTAAGTTCAACATTGAATAAAGTTCTTTTTCCTTTTTTCTTTTGAAAATTCTAAAGAGGGTCGATGTGAGATTGCGTTATCCGTATCAGTGTGATGTTCTATCCCGAACAAACGACTGTTGTCAGTTGAGATCGAAGAAAAAAAATCCTATTTAAACACATTGTAGGAGGGGGCCATTTATCTAACAGCATATAACAAGTTCCAAGAAGAAAAATAGAACTTCAGCAATTCTTCATTGCTATCAATGAAATTCAATGGGTATTTTGTGGAGTCGATGTAATCTTGACTTACTAGTTGGTTTAGTACTCTTCAAATGCTTACTCTCGACCGTTTATAGTTTAGAATATGATTCGTGTTAGGAAGTCTTGGTCCATTCTTGCTTACGAAGACTTTCGTTGTGGATTTTATCGTCACATTAGCATACGTTTTAATAAATGATATTGTTCAAGTTCCTCATGGTTTCTGAAATGGCTACTTTTTATTTACCTTATTGCTTGCTAGATCCTAGATGTGGCTGTTTCGCTTGCGAAAGTTGCAGATGTGGACAGTGGTCTAGGAAATGAGGATATGGCAGTTAATGGGTTCGAGGAAGGCATAAAGTTATTGGAATCGTTAACACTGAACTCGGAGAATCCTGGTCTTGAGCAGCGAGTAAGGCTACAATTCTTTGTTTCCGAACCTTTATGCATCATGTTTTAATTAGTTGAACTATATTTGAGTTAATCGACATTATTTCTATTTTGCTCGTAGCGTCAATCTGTGTTGAAATTCCTCGAAGGTCAACTTGCAGAGCGACAAAAGGCAACTCAATCCAGTTGA

mRNA sequence

ATGACTCCTGTTTGTCCCTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGCCGGAATCTGCTTGTCCTTTTGCGAAATCCGGCCGTTCCGATGATGCTTCTTCACGAAAACAGCAATCATCGGAGTCTTCTTGTCCGTTTTCGAAATCTGCTCGTTCTGACGACGGTTCTTTGAAGAAGGATCAGCCTGAGGCGGAGAAAGACGTTGCCGATGCTGCTAAAACTGGCGGCAAATGTCCGTTCGGATATGATTCTCAAACTTTCAAGATTGGTCCTCTAAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAAGCATGTATATCACGCTTTAATGATTGCCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCACACGCTGATCTGCAAGCCACGGTCGATCGATTCATTGAAGGTCATGCGAGAATCAAGAGATCTCAGATGAACTCAGACAAAGAGCAGGAGGAAGTTAGTGAAAGTAAAGTGGTTATTTATGAAGATTTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGCATTTCGTGCCCAGAATATTGAAAGTGCCAAATCCAGGCTCACTATCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAGCGGGCAATTCATCAGAATTATGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGTCGAGCAGCTGGGGATGCTAGTTCTGCAATAAAGCACTTCGAAGAAAGTGTAGAGTTTCTCTCAAAACTACCCGAAAAGACTCGCGAGGTCACGCATACACTTTCTGTTTCACTAAATAAAATTGGCGATCTTAAATATTATGAAGGAGACCTTCAAGCTGCAAGATCTTATTATCAGCAGTCTCTTAATGTTCGCCAAGATGCAAGCAAGCATCATTCAGAAGATCCATCCCAGATCCTAGATGTGGCTGTTTCGCTTGCGAAAGTTGCAGATGTGGACAGTGGTCTAGGAAATGAGGATATGGCAGTTAATGGGTTCGAGGAAGGCATAAAGTTATTGGAATCGTTAACACTGAACTCGGAGAATCCTGGTCTTGAGCAGCGACGTCAATCTGTGTTGAAATTCCTCGAAGGTCAACTTGCAGAGCGACAAAAGGCAACTCAATCCAGTTGA

Coding sequence (CDS)

ATGACTCCTGTTTGTCCCTTCGTGAAATCTGCTCGTCCCGACGACGCTTCTTCTCGGAAGCACCAGCCGGAATCTGCTTGTCCTTTTGCGAAATCCGGCCGTTCCGATGATGCTTCTTCACGAAAACAGCAATCATCGGAGTCTTCTTGTCCGTTTTCGAAATCTGCTCGTTCTGACGACGGTTCTTTGAAGAAGGATCAGCCTGAGGCGGAGAAAGACGTTGCCGATGCTGCTAAAACTGGCGGCAAATGTCCGTTCGGATATGATTCTCAAACTTTCAAGATTGGTCCTCTAAGCTGTATGATTTGTCAGGCACTTCTCTTTGAATGCAGCAGATGTGTCCCCTGTTCTCACATTTTCTGCAAAGCATGTATATCACGCTTTAATGATTGCCCCCTGTGTGGAGCTGACATTGAGAAGATTGAAGCACACGCTGATCTGCAAGCCACGGTCGATCGATTCATTGAAGGTCATGCGAGAATCAAGAGATCTCAGATGAACTCAGACAAAGAGCAGGAGGAAGTTAGTGAAAGTAAAGTGGTTATTTATGAAGATTTGTCCATGGAGAGAGGTGCTTTCTTGATCCAACAAGCCATGAGGGCATTTCGTGCCCAGAATATTGAAAGTGCCAAATCCAGGCTCACTATCTGTGTTGAAGATATTCGAGATCAATTAGAAAGAGCGGGCAATTCATCAGAATTATGCTCACAGCTTGGAGCAGTTCTAGGCACACTTGGTGATTGTTGTCGAGCAGCTGGGGATGCTAGTTCTGCAATAAAGCACTTCGAAGAAAGTGTAGAGTTTCTCTCAAAACTACCCGAAAAGACTCGCGAGGTCACGCATACACTTTCTGTTTCACTAAATAAAATTGGCGATCTTAAATATTATGAAGGAGACCTTCAAGCTGCAAGATCTTATTATCAGCAGTCTCTTAATGTTCGCCAAGATGCAAGCAAGCATCATTCAGAAGATCCATCCCAGATCCTAGATGTGGCTGTTTCGCTTGCGAAAGTTGCAGATGTGGACAGTGGTCTAGGAAATGAGGATATGGCAGTTAATGGGTTCGAGGAAGGCATAAAGTTATTGGAATCGTTAACACTGAACTCGGAGAATCCTGGTCTTGAGCAGCGACGTCAATCTGTGTTGAAATTCCTCGAAGGTCAACTTGCAGAGCGACAAAAGGCAACTCAATCCAGTTGA

Protein sequence

MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDDGSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Homology
BLAST of Csor.00g300950 vs. ExPASy Swiss-Prot
Match: Q9M2V1 (Protein NCA1 OS=Arabidopsis thaliana OX=3702 GN=NCA1 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-140
Identity = 265/395 (67.09%), Postives = 313/395 (79.24%), Query Frame = 0

Query: 2   TPVCPFVKSARPDDASSRKHQ--PESACPFAKSGRSDDASSRKQ-QSSESSCPFSKSARS 61
           T VCPF K+ARPDD S+RK      S CPF+K+ R DDAS+RKQ +++ S CPFSKSAR 
Sbjct: 4   TSVCPFSKAARPDDGSTRKQGEITASGCPFSKAARPDDASARKQGETTASGCPFSKSARP 63

Query: 62  DDGSLKKDQPEAE----KDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 121
           D+    K  PE E    KD  D+A    KCPFGYDSQTFK+GP SCM+CQALL+E SRCV
Sbjct: 64  DENG-SKGCPEQEGNLNKDSTDSATVPAKCPFGYDSQTFKLGPFSCMLCQALLYESSRCV 123

Query: 122 PCSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEE 181
           PC+H+FCK C++RF DCPLCGADIE IE   +LQ  VD+FIEGHARIKRS +N  +++E 
Sbjct: 124 PCTHVFCKVCLTRFKDCPLCGADIESIEVDENLQKMVDQFIEGHARIKRSVVNGTEKEEV 183

Query: 182 VSESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSEL 241
            +++K VIY D+SMERG+FL+QQAMRAF AQN ESAKSRL +C EDIRDQL R GN+ EL
Sbjct: 184 ENDNKKVIYADVSMERGSFLVQQAMRAFSAQNYESAKSRLAMCTEDIRDQLGREGNTPEL 243

Query: 242 CSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLK 301
           CSQLGAVLG LGDC RA GD+SSA+KHFEESVEFL KLP    E+THTLSVSLNKIGDLK
Sbjct: 244 CSQLGAVLGMLGDCSRAMGDSSSAVKHFEESVEFLMKLPLNDLEITHTLSVSLNKIGDLK 303

Query: 302 YYEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNG 361
           YY+ DLQAARSYY ++LNVR+DA KHH   PSQILDVAVSLAKVAD+D  L NE  A +G
Sbjct: 304 YYDEDLQAARSYYDRALNVRRDAMKHHPNAPSQILDVAVSLAKVADIDRTLQNEVAATDG 363

Query: 362 FEEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQL 390
           F+EG++LLESL L+SE+  LEQRR SVL+FL+ Q+
Sbjct: 364 FKEGMRLLESLKLDSEDSALEQRRLSVLEFLKKQV 397

BLAST of Csor.00g300950 vs. ExPASy Swiss-Prot
Match: A5WW08 (E3 ubiquitin-protein ligase CHFR OS=Danio rerio OX=7955 GN=chfr PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.0e-04
Identity = 38/137 (27.74%), Postives = 62/137 (45.26%), Query Frame = 0

Query: 33  GRSDDASSRKQQSSESSCPFSKSARSDDGSLKKDQPEAEKDVADAAKTGGKCPFGYDSQT 92
           G  ++   RK++ ++   P   SA SD          A  D+     +G +   G  +  
Sbjct: 221 GEPEEEMQRKRRKTDRDDPGFGSAHSD----------ASADIPLRGASGKEKTEGATTDK 280

Query: 93  FKIGPLSCMICQALLFECSRCVPCSHIFCKACIS----RFNDCPLCGADIEKIEAHADLQ 152
            +   L+C+ICQ LL++C    PC H FC AC S    R + CP C   +E+I  +  L 
Sbjct: 281 ME-ESLTCIICQDLLYDCISVQPCMHTFCAACYSGWMERSSFCPTCRCPVERIRKNHILN 340

Query: 153 ATVDRFIEGHARIKRSQ 166
             V+ ++  H    R++
Sbjct: 341 NLVEAYLLQHPEKCRTE 346

BLAST of Csor.00g300950 vs. ExPASy Swiss-Prot
Match: Q5FWP4 (E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 7.7e-04
Identity = 45/164 (27.44%), Postives = 68/164 (41.46%), Query Frame = 0

Query: 6   PFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDDGSLKK 65
           P ++++ P    S + Q  S  P  K  R++D  +  +    +SCP   S  S   S+K 
Sbjct: 199 PAIRASIPKSNLSTQEQ-GSLGPPKKRIRTEDHWTTNKNFVPASCPIGASDESKTPSMKP 258

Query: 66  DQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIFCKACI 125
           D+ E                            L+C+ICQ LL +C    PC H FC AC 
Sbjct: 259 DKMEE--------------------------TLTCIICQELLHDCVSLQPCMHTFCAACY 318

Query: 126 S----RFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQ 166
           S    R + CP C   +E+I  +  L   V+ ++  H    RS+
Sbjct: 319 SGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPEKCRSE 335

BLAST of Csor.00g300950 vs. NCBI nr
Match: XP_022945180.1 (protein NCA1-like [Cucurbita moschata] >KAG6573779.1 Protein NCA1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 771 bits (1991), Expect = 1.02e-280
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
           GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. NCBI nr
Match: KAG7012853.1 (Protein NCA1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 768 bits (1984), Expect = 1.18e-279
Identity = 397/399 (99.50%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
           GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYYQQSLNVRQ++SKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYQQSLNVRQESSKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. NCBI nr
Match: XP_023542121.1 (protein NCA1-like [Cucurbita pepo subsp. pepo] >XP_023542122.1 protein NCA1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768 bits (1982), Expect = 2.39e-279
Identity = 397/399 (99.50%), Postives = 398/399 (99.75%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
            SLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  ASLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLE+LTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLEALTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. NCBI nr
Match: XP_022966763.1 (protein NCA1 [Cucurbita maxima])

HSP 1 Score: 766 bits (1978), Expect = 9.73e-279
Identity = 397/399 (99.50%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
            SLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  ASLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYY QSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYLQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. NCBI nr
Match: XP_022139165.1 (protein NCA1 isoform X1 [Momordica charantia] >XP_022139166.1 protein NCA1 isoform X1 [Momordica charantia])

HSP 1 Score: 707 bits (1826), Expect = 1.58e-255
Identity = 367/399 (91.98%), Postives = 381/399 (95.49%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPE+ACPFAKSGRSDDASSRKQQS E++CPFSKSAR DD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPEAACPFAKSGRSDDASSRKQQS-EAACPFSKSARCDD 60

Query: 61  GSLKKDQPEAE-----KDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
            S++K+Q EAE     KDVADAA TGGKCPFGYDSQTFK+GPLSCMICQALLFECSRCVP
Sbjct: 61  ASVRKNQVEAESNEAEKDVADAATTGGKCPFGYDSQTFKLGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEV 180
           CSHI+CKACISRF DCPLCGADIEKIEAHADLQ TVDRFIEGHARIKRSQ+NSDKEQEEV
Sbjct: 121 CSHIYCKACISRFKDCPLCGADIEKIEAHADLQGTVDRFIEGHARIKRSQVNSDKEQEEV 180

Query: 181 SESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELC 240
           SESKVVIYED+SMERGAFLIQQAMRAFRAQN ESAKSRLTICVEDIRDQLER GNSSELC
Sbjct: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNFESAKSRLTICVEDIRDQLERMGNSSELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEK+ EVTHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKSHEVTHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGF 360
           YEGDLQAARSYY +SLNVRQDASKHH +DPSQILDVAVSLAKVADVDSGLGNEDMAVNGF
Sbjct: 301 YEGDLQAARSYYFRSLNVRQDASKHHPDDPSQILDVAVSLAKVADVDSGLGNEDMAVNGF 360

Query: 361 EEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLAERQK 394
           +EGIKLLESLTLNSENPGLEQRRQSVLKFLEGQL ERQK
Sbjct: 361 QEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLTERQK 398

BLAST of Csor.00g300950 vs. ExPASy TrEMBL
Match: A0A6J1G074 (protein NCA1-like OS=Cucurbita moschata OX=3662 GN=LOC111449499 PE=4 SV=1)

HSP 1 Score: 771 bits (1991), Expect = 4.92e-281
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
           GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. ExPASy TrEMBL
Match: A0A6J1HT54 (protein NCA1 OS=Cucurbita maxima OX=3661 GN=LOC111466377 PE=4 SV=1)

HSP 1 Score: 766 bits (1978), Expect = 4.71e-279
Identity = 397/399 (99.50%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60

Query: 61  GSLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120
            SLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF
Sbjct: 61  ASLKKDQPEAEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVPCSHIF 120

Query: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180
           CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV
Sbjct: 121 CKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEVSESKV 180

Query: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240
           VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA
Sbjct: 181 VIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELCSQLGA 240

Query: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300
           VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL
Sbjct: 241 VLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKYYEGDL 300

Query: 301 QAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360
           QAARSYY QSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK
Sbjct: 301 QAARSYYLQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGFEEGIK 360

Query: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS
Sbjct: 361 LLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399

BLAST of Csor.00g300950 vs. ExPASy TrEMBL
Match: A0A6J1CBV4 (protein NCA1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010138 PE=4 SV=1)

HSP 1 Score: 707 bits (1826), Expect = 7.65e-256
Identity = 367/399 (91.98%), Postives = 381/399 (95.49%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQPE+ACPFAKSGRSDDASSRKQQS E++CPFSKSAR DD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQPEAACPFAKSGRSDDASSRKQQS-EAACPFSKSARCDD 60

Query: 61  GSLKKDQPEAE-----KDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCVP 120
            S++K+Q EAE     KDVADAA TGGKCPFGYDSQTFK+GPLSCMICQALLFECSRCVP
Sbjct: 61  ASVRKNQVEAESNEAEKDVADAATTGGKCPFGYDSQTFKLGPLSCMICQALLFECSRCVP 120

Query: 121 CSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEEV 180
           CSHI+CKACISRF DCPLCGADIEKIEAHADLQ TVDRFIEGHARIKRSQ+NSDKEQEEV
Sbjct: 121 CSHIYCKACISRFKDCPLCGADIEKIEAHADLQGTVDRFIEGHARIKRSQVNSDKEQEEV 180

Query: 181 SESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSELC 240
           SESKVVIYED+SMERGAFLIQQAMRAFRAQN ESAKSRLTICVEDIRDQLER GNSSELC
Sbjct: 181 SESKVVIYEDVSMERGAFLIQQAMRAFRAQNFESAKSRLTICVEDIRDQLERMGNSSELC 240

Query: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLKY 300
           SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEK+ EVTHTLSVSLNKIGDLKY
Sbjct: 241 SQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKSHEVTHTLSVSLNKIGDLKY 300

Query: 301 YEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNGF 360
           YEGDLQAARSYY +SLNVRQDASKHH +DPSQILDVAVSLAKVADVDSGLGNEDMAVNGF
Sbjct: 301 YEGDLQAARSYYFRSLNVRQDASKHHPDDPSQILDVAVSLAKVADVDSGLGNEDMAVNGF 360

Query: 361 EEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLAERQK 394
           +EGIKLLESLTLNSENPGLEQRRQSVLKFLEGQL ERQK
Sbjct: 361 QEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLTERQK 398

BLAST of Csor.00g300950 vs. ExPASy TrEMBL
Match: A0A0A0KPN8 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G581690 PE=4 SV=1)

HSP 1 Score: 679 bits (1751), Expect = 2.19e-244
Identity = 355/405 (87.65%), Postives = 379/405 (93.58%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQ ESACPFAKSGRSDDASSRK QS ES+CP SKS RSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQS-ESACPVSKSVRSDD 60

Query: 61  GS-LKKDQPEAE-----KDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 120
            S LKK+Q EAE     KDVADAA  GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV
Sbjct: 61  ASSLKKNQAEAESNGAEKDVADAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 120

Query: 121 PCSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEE 180
           PC+HIFCKACISRFNDCPLCGADIEKIEA  +LQATVDRFIEGHARIKR Q+NSDKEQE+
Sbjct: 121 PCTHIFCKACISRFNDCPLCGADIEKIEADDNLQATVDRFIEGHARIKRPQVNSDKEQEK 180

Query: 181 VSESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSEL 240
           V+ESKVVIYED+SMERGAFLIQQAMRAFRAQNIESAKSRLT+CVEDIRDQLE+ G+S EL
Sbjct: 181 VNESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPEL 240

Query: 241 CSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLK 300
           CSQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKT E+THTLSVSLNKIGDLK
Sbjct: 241 CSQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLK 300

Query: 301 YYEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNG 360
           YYEGDL+AARSYY +SLNVRQDASKHH +DP+QILDVAVSLAKVADVDSGLGNEDMAV+ 
Sbjct: 301 YYEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILDVAVSLAKVADVDSGLGNEDMAVDR 360

Query: 361 FEEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           F+EGIKLLESL+LNSENP L+ RR+SV+KFLEGQLAERQKATQ+S
Sbjct: 361 FQEGIKLLESLSLNSENPALQHRRESVMKFLEGQLAERQKATQTS 404

BLAST of Csor.00g300950 vs. ExPASy TrEMBL
Match: A0A5D3CV76 (Binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003220 PE=4 SV=1)

HSP 1 Score: 677 bits (1747), Expect = 8.92e-244
Identity = 354/405 (87.41%), Postives = 378/405 (93.33%), Query Frame = 0

Query: 1   MTPVCPFVKSARPDDASSRKHQPESACPFAKSGRSDDASSRKQQSSESSCPFSKSARSDD 60
           MTPVCPFVKSARPDDASSRKHQ ESACPFAKSGRSDDASSRK QS ES+CPFSKS RSDD
Sbjct: 1   MTPVCPFVKSARPDDASSRKHQGESACPFAKSGRSDDASSRKPQS-ESACPFSKSVRSDD 60

Query: 61  GS-LKKDQPE-----AEKDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 120
            S L+K+Q E     AEKDV DAA  GGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV
Sbjct: 61  ASSLEKNQAEGESNGAEKDVGDAAGAGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 120

Query: 121 PCSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEE 180
           PCSHIFCKACISRFNDCPLCGADIEK+EA  +LQATVDRFIEGHARIKR Q+NSDKEQE+
Sbjct: 121 PCSHIFCKACISRFNDCPLCGADIEKVEADDNLQATVDRFIEGHARIKRPQVNSDKEQEK 180

Query: 181 VSESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSEL 240
           V+ESKVVIYED+SMERGAFLIQQAMRAFRAQNIESAKSRLT+CVEDIRDQLE+ G+S EL
Sbjct: 181 VNESKVVIYEDVSMERGAFLIQQAMRAFRAQNIESAKSRLTVCVEDIRDQLEKMGSSPEL 240

Query: 241 CSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLK 300
           CSQLGAVLGTLGDCCRAAGDA+SAIKHFEESVEFLSKLPEKT E+THTLSVSLNKIGDLK
Sbjct: 241 CSQLGAVLGTLGDCCRAAGDAASAIKHFEESVEFLSKLPEKTHEITHTLSVSLNKIGDLK 300

Query: 301 YYEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNG 360
           YYEGDL+AARSYY +SLNVRQDASKHH +DP+QILDVAVSLAKVADVDSGLGNEDMAV+ 
Sbjct: 301 YYEGDLEAARSYYLRSLNVRQDASKHHPDDPAQILDVAVSLAKVADVDSGLGNEDMAVDR 360

Query: 361 FEEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQLAERQKATQSS 399
           F+EGIKLLESL+LNSENP L+ R +SVLKFLEGQLAERQKATQ+S
Sbjct: 361 FQEGIKLLESLSLNSENPALQHRGESVLKFLEGQLAERQKATQTS 404

BLAST of Csor.00g300950 vs. TAIR 10
Match: AT3G54360.1 (zinc ion binding )

HSP 1 Score: 501.1 bits (1289), Expect = 8.1e-142
Identity = 265/395 (67.09%), Postives = 313/395 (79.24%), Query Frame = 0

Query: 2   TPVCPFVKSARPDDASSRKHQ--PESACPFAKSGRSDDASSRKQ-QSSESSCPFSKSARS 61
           T VCPF K+ARPDD S+RK      S CPF+K+ R DDAS+RKQ +++ S CPFSKSAR 
Sbjct: 4   TSVCPFSKAARPDDGSTRKQGEITASGCPFSKAARPDDASARKQGETTASGCPFSKSARP 63

Query: 62  DDGSLKKDQPEAE----KDVADAAKTGGKCPFGYDSQTFKIGPLSCMICQALLFECSRCV 121
           D+    K  PE E    KD  D+A    KCPFGYDSQTFK+GP SCM+CQALL+E SRCV
Sbjct: 64  DENG-SKGCPEQEGNLNKDSTDSATVPAKCPFGYDSQTFKLGPFSCMLCQALLYESSRCV 123

Query: 122 PCSHIFCKACISRFNDCPLCGADIEKIEAHADLQATVDRFIEGHARIKRSQMNSDKEQEE 181
           PC+H+FCK C++RF DCPLCGADIE IE   +LQ  VD+FIEGHARIKRS +N  +++E 
Sbjct: 124 PCTHVFCKVCLTRFKDCPLCGADIESIEVDENLQKMVDQFIEGHARIKRSVVNGTEKEEV 183

Query: 182 VSESKVVIYEDLSMERGAFLIQQAMRAFRAQNIESAKSRLTICVEDIRDQLERAGNSSEL 241
            +++K VIY D+SMERG+FL+QQAMRAF AQN ESAKSRL +C EDIRDQL R GN+ EL
Sbjct: 184 ENDNKKVIYADVSMERGSFLVQQAMRAFSAQNYESAKSRLAMCTEDIRDQLGREGNTPEL 243

Query: 242 CSQLGAVLGTLGDCCRAAGDASSAIKHFEESVEFLSKLPEKTREVTHTLSVSLNKIGDLK 301
           CSQLGAVLG LGDC RA GD+SSA+KHFEESVEFL KLP    E+THTLSVSLNKIGDLK
Sbjct: 244 CSQLGAVLGMLGDCSRAMGDSSSAVKHFEESVEFLMKLPLNDLEITHTLSVSLNKIGDLK 303

Query: 302 YYEGDLQAARSYYQQSLNVRQDASKHHSEDPSQILDVAVSLAKVADVDSGLGNEDMAVNG 361
           YY+ DLQAARSYY ++LNVR+DA KHH   PSQILDVAVSLAKVAD+D  L NE  A +G
Sbjct: 304 YYDEDLQAARSYYDRALNVRRDAMKHHPNAPSQILDVAVSLAKVADIDRTLQNEVAATDG 363

Query: 362 FEEGIKLLESLTLNSENPGLEQRRQSVLKFLEGQL 390
           F+EG++LLESL L+SE+  LEQRR SVL+FL+ Q+
Sbjct: 364 FKEGMRLLESLKLDSEDSALEQRRLSVLEFLKKQV 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2V11.1e-14067.09Protein NCA1 OS=Arabidopsis thaliana OX=3702 GN=NCA1 PE=1 SV=1[more]
A5WW082.0e-0427.74E3 ubiquitin-protein ligase CHFR OS=Danio rerio OX=7955 GN=chfr PE=2 SV=1[more]
Q5FWP47.7e-0427.44E3 ubiquitin-protein ligase CHFR OS=Xenopus laevis OX=8355 GN=chfr PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022945180.11.02e-280100.00protein NCA1-like [Cucurbita moschata] >KAG6573779.1 Protein NCA1, partial [Cucu... [more]
KAG7012853.11.18e-27999.50Protein NCA1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023542121.12.39e-27999.50protein NCA1-like [Cucurbita pepo subsp. pepo] >XP_023542122.1 protein NCA1-like... [more]
XP_022966763.19.73e-27999.50protein NCA1 [Cucurbita maxima][more]
XP_022139165.11.58e-25591.98protein NCA1 isoform X1 [Momordica charantia] >XP_022139166.1 protein NCA1 isofo... [more]
Match NameE-valueIdentityDescription
A0A6J1G0744.92e-281100.00protein NCA1-like OS=Cucurbita moschata OX=3662 GN=LOC111449499 PE=4 SV=1[more]
A0A6J1HT544.71e-27999.50protein NCA1 OS=Cucurbita maxima OX=3661 GN=LOC111466377 PE=4 SV=1[more]
A0A6J1CBV47.65e-25691.98protein NCA1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010138 PE=4 SV=1[more]
A0A0A0KPN82.19e-24487.65RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G581690 P... [more]
A0A5D3CV768.92e-24487.41Binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00322... [more]
Match NameE-valueIdentityDescription
AT3G54360.18.1e-14267.09zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 284..317
e-value: 12.0
score: 14.2
coord: 239..272
e-value: 26.0
score: 11.3
NoneNo IPR availablePFAMPF13424TPR_12coord: 240..315
e-value: 5.1E-9
score: 36.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..56
NoneNo IPR availablePANTHERPTHR15315RING FINGER PROTEIN 41, 151coord: 1..392
NoneNo IPR availablePANTHERPTHR15315:SF89PROTEIN NCA1coord: 1..392
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 95..141
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 90..184
e-value: 4.7E-9
score: 38.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 203..377
e-value: 3.6E-13
score: 51.1
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 233..367
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 116..125
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 100..134
score: 11.486073

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g300950.m01Csor.00g300950.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902553 positive regulation of catalase activity
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity