Csor.00g299410 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g299410
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	tRNA 4-demethylwyosine synthase (AdoMet-dependent)
Location	Csor_Chr05: 1083300 .. 1086609 (-)
RNA-Seq Expression	Csor.00g299410
Synteny	Csor.00g299410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGTAATTTCTTCGGCTGTTTATCTGCATTTTATTTCTCACGATGAGATGATGAAGAAAGCTTTCGGCAGACATTACCTTTAATCATAGATTCCAAATTTGTGTTTTCCGGTGCACCCCCAAATTGTCGGAATTACTGCTTGTTTTCTAGGCAATTTCTGGGTGCCTTGTGAATCAATACAAATCTGGAATGACTTGATAGTTTATCTTTTGCAACGGTCTTAGTTGAAGCTTTACTTCTTTTCCATTTGTTGTCTGATTGGCTCTTGAAATGGTTTTTCTTATGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGGTAATGTCCCATACATTCTATTTTGCTTGACCACAGCGAGTCTTCTTTCCTTTTCACGGTCATTATAATATTTGATGTTTTGATACATTTAGTGGCAATGACATAGAACTCTCCGTTTCATAGATTAGAATTAAGATGTATGCCTCTTTACTCTGGTAAATGAATGATTTCTTTTCTTAGGAGGATGGATGATAGAATATGATTGAAGACTGCTAGGTGAATGGAAAAATAAGAATTTGGTGGCAGTGGCCTTCAAATCACTGAAAGTTACTAGAGTGTTTTTGGTGGCATCAGAGAAACTTTTGTTTGATATTCTTTGCTCGGCCAAATTTTACATCCAGCTGCTACTGATTTTATTTTTGAAATCCTTACTGTTTTGCCTACGTGACTGATATCTTCAATTTCCATAACATAAGCAGAAAGTTTCATGCTTTCTCATATTTTCTCAGTACATCCTTTTTATGTTGTTATGCTGATGCAAAATGTATATTGAGAGATAATTTTCTTTGATTTATCAACTTGCATTATGAATCCTTCTTCATCTGGGAACTTGAATTTAAAGTTGACTCCTCACTTTCCTTCCACACAACAAGGCTTTTCCTAATCTCTTAATTTTTTTTATAGACTGGTAGAAACTATCTATTGATTAGTGCTGTTGCTTGGATTAACTGTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCGAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCAGCAATCGATTTACACAAAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTTAGTGCTCCTGTTCTACTGCACTGGTGTGAGTAATATTTTCATGAAGATTGACCTTTCTTGGATAGACTTAATTGCTCCTGTGATTTGATGGTTGTAGAATATGATTTTTTTTTTTTTTGTTTGTAAATCATCAATATTGTTAATGTTTGTTATTTCTTTTAAAACTGTCAAAGAAATGCGCTGGCATTGCATGAGTTTTATGGCTAAATAGATATCAAGAGTAGTAGACCACAAAGTAACGGCATTTTATACAGTTGAATCCCCCTTTGGGAGTTATAGAGATCTTTTAAGGAAGAAGTTCAAGTAATATATTAATCTAAGATTCCTGAAAATTTGGCTGCTTGAGAATTTGAAGTTGCAGTATTTTATTTTATAGTACCTATCATTGAGAATCCTGAGAGAATGCTTGAAATGAAAGTTATTTTTTAAATTTCCTTTTATTATTATTGTTATTATTTGTTTTTTTTTTTTTTTTTTTTTTGCAGTAATCCATGAAATTTTATCGATGAGTTTATGTTCACGTAACCTTTTTTTTTTAATTGTTGGAAAAAGGTCATGTCAACGAATTTGAAAGACAATGACCAACGCTTGCTCCTTTACACAAACAAGTATAAAGCTAATTTTAGTAATCAGATATACTCATAACGTAAAATAAAAGTTCATGTACAATGCTAAAAAGTAAAAGCAAATTTTCTTTCGTTGGTAGTTGGGATTTTAGTGATGATGTGATTACAGAGTCATTAATGCATATCAATCTTGTTGATGAGTGATGTCTTCCTGCAGGTGTTACGCAGGAGAAATTGGAAGAAGGCTTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCACCGAAGGCGGATTTCAACTTTCTTAGTTACAAATGCTCAGTTTCCTGATAAAATAAAATCGTTGAAGCCGGTCACCCAGGTTTGCATGCTTTACAAATTTTCTTTTCTAAATAACATCAAATGTCAGGCTTATGCTCTTTGATAACTAGATTACCTAGCTGATTTCAATGATGCATGCAGTTGTATGTAAGTGTAGATGCTGCAACGAAGGATAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGACTTTTGGGAGCGATTCATTGTAAGTTTCCTCCTATCCTCACCTGTTTCCAGGCCTGTCTCCTGA

mRNA sequence

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCGAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCAGCAATCGATTTACACAAAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGAAGAAGGCTTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCACCGAAGGCGGATTTCAACTTTCTTAGTTACAAATGCTCAGTTTCCTGATAAAATAAAATCGTTGAAGCCGGTCACCCAGTTGTATGTAAGTGTAGATGCTGCAACGAAGGATAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGACTTTTGGGAGCGATTCATTGTAAGTTTCCTCCTATCCTCACCTGTTTCCAGGCCTGTCTCCTGA

Coding sequence (CDS)

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCGAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCAGCAATCGATTTACACAAAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGAAGAAGGCTTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCACCGAAGGCGGATTTCAACTTTCTTAGTTACAAATGCTCAGTTTCCTGATAAAATAAAATCGTTGAAGCCGGTCACCCAGTTGTATGTAAGTGTAGATGCTGCAACGAAGGATAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGACTTTTGGGAGCGATTCATTGTAAGTTTCCTCCTATCCTCACCTGTTTCCAGGCCTGTCTCCTGA

Protein sequence

MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVSFLLSSPVSRPVS

Homology

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BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.1e-187
Identity = 335/476 (70.38%), Postives = 385/476 (80.88%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLK-LSLNPTGC--SRRPKLFFISQTGTSKAL 63
           SS   +LA +ALLSA+T YC +K RRL+ LK LSLNP+    + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 123
           A RL  +  +N++AFDIVDP  +EPEDL KETLVLF+ASTW+ G PP + +FL +WL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRQLLKVLKVDAV--GTGADLYSGIDED----FDAESVEEDDEDADSGEED 243
           ++D+ F DW   +++VLK  +     G     G  ED    +D+   E++D DAD G   
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGG--- 244

Query: 244 IVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRW 303
           IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRW
Sbjct: 245 IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRW 304

Query: 304 TKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL 363
           TKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P 
Sbjct: 305 TKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPS 364

Query: 364 DIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHR 423
            IV  A+DLHK MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH 
Sbjct: 365 VIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHG 424

Query: 424 RRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 471
           RRISTFLVTNAQFP+KI  +KP+TQLYVSVDAATK+SLKAIDRPLF DFWERFI S
Sbjct: 425 RRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDS 475

[Close]

BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 9.7e-171
Identity = 311/475 (65.47%), Postives = 365/475 (76.84%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNP--TGCSRRPKLFFISQTGTSKALA 63
           +S ++ LALL LLS+S+++  YKS RLR+      P   G +  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSESA 123
             L   L    +     D   F+P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGD 183
            DFR G+ LLS  +FAVFGVGSRAYGETFNA AR FS+ ++ALGA E++AVGEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEED--IVDLE 243
           ++  F++W  ++++V+K + +G G   ++G  + FD    EE D+D D  E D   VD+E
Sbjct: 192 LEVVFEEWCGRVVRVVKGEEIGEG---HNGESDGFDELEEEESDDDDDEEEVDGGEVDME 251

Query: 244 DIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWT 303
           DIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKICRWT
Sbjct: 252 DIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWT 311

Query: 304 KSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLD 363
           KSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDDPLD
Sbjct: 312 KSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLD 371

Query: 364 IVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRR 423
           IVN+AID H KM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRR
Sbjct: 372 IVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRR 431

Query: 424 RISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 471
            ISTFLVTNAQFPDKIK+LKP+TQLYVSVDAATK+SLKA+DRPLF DFWERF+ S
Sbjct: 432 HISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDS 483

[Close]

BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 6.6e-119
Identity = 231/481 (48.02%), Postives = 306/481 (63.62%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +    L  ++++ K+++P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG PP  A++   WL E++ DFR G   L   ++AVFG+G+  Y   +N V ++  K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRQLLKVLKVDAVG------------- 226
             L A  ++   EGD +      G I+  F+ W R+ L  LK    G             
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGK 263

Query: 227 ------TGADLYSGIDEDFDAESVEEDDED------ADSGEED-------IVDLEDIAGK 286
                 +  +     +E+ +  S  ED ED      +DS  E+       ++D+ED+ GK
Sbjct: 264 CKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-GK 323

Query: 287 GPS--RKSTNVVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSGV 346
             S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSGV
Sbjct: 324 AMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGV 383

Query: 347 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWE 406
           K+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+
Sbjct: 384 KLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWK 443

Query: 407 MDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLV 466
           MD P  I+  AI  H+ MIKQ KGVPGV  E+ EEGL+ +HCALSLVGEPIMYPEINT +
Sbjct: 444 MDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFL 503

Query: 467 DELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIV 471
             LH + IS+FLVTNAQFP++I+SL+PVTQLYVSVDA+TKDSLK IDRPLF DFW+RFI 
Sbjct: 504 RLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFID 562

[Close]

BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.0e-116
Identity = 222/478 (46.44%), Postives = 295/478 (61.72%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPEDLFKE-----TLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + A  +  +++D KDF+PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+  Y   FN V++   K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRQLLKVLKVDAVG------------- 226
             L A  I+  GEGD +      G +   F  W  + L  L+  A G             
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKAS 259

Query: 227 ---------TGADLYSGIDEDFDAESVEEDDEDADSGEED------IVDLEDIAG----- 286
                       D +S  +++   E + E   D +S  ED      ++D+ED+       
Sbjct: 260 CKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHM 319

Query: 287 -KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKIC 346
            K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+ 
Sbjct: 320 KKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLR 379

Query: 347 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 406
           RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD 
Sbjct: 380 RWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDP 439

Query: 407 PLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 466
              I+  A++ H+ MI+Q +GVPGV  E+ EEGL+ +HCALSLVGEPIMYPEIN+ +  L
Sbjct: 440 AEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLL 499

Query: 467 HRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 471
           H++ IS+FLVTNAQFP++I+SL PVTQLYVSVDA+TKDSLK IDRPLF DFW+RF+ S
Sbjct: 500 HQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 557

[Close]

BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 416.4 bits (1069), Expect = 4.4e-115
Identity = 219/480 (45.62%), Postives = 293/480 (61.04%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + +L   I++ K+++P+D     +  + + +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+ AY   FN V ++  K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSG----- 226
             LGA  +++ GEGD D      G I+  F  W  + +  L+    G       G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 ----------------------------IDEDFDAESVEEDDEDADSGEEDIVDLEDIAG 286
                                        +E F++ S EE   +       IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H+ MIKQ KGVPGV  E+ EEG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 471
            LH+ +IS+FLVTNAQFP +I++L+PVTQLYVSVDA+TKDSLK IDRPLF DFW+RF+ S
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

[Close]

BLAST of Csor.00g299410 vs. NCBI nr
Match: KAG6598440.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVSFLLSSPVSRP 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVSFLLSSPVSRP
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVSFLLSSPVSRP 480

Query: 481 VS 482
           VS
Sbjct: 481 VS 482

[Close]

BLAST of Csor.00g299410 vs. NCBI nr
Match: XP_023546392.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 466/470 (99.15%), Postives = 469/470 (99.79%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           LVTNAQFPDKIKSLKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 470

[Close]

BLAST of Csor.00g299410 vs. NCBI nr
Match: XP_022962153.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita moschata])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 466/470 (99.15%), Postives = 467/470 (99.36%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFI S
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDS 470

[Close]

BLAST of Csor.00g299410 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 927 bits (2396), Expect = 0.0
Identity = 463/470 (98.51%), Postives = 466/470 (99.15%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKE LVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           LVTNAQFPDKIKSLKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 470

[Close]

BLAST of Csor.00g299410 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 446/471 (94.69%), Postives = 457/471 (97.03%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT  SRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NEL FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLS+CKFAVFGVGSRAYGETFNAVAR+FSKRMKALGAKEILAVG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDE-DADSGEEDIVDLE 240
           DID+CFDDWSRQLLKVLKVDAVG G DLYSGI  D DAESVEED+E DAD GEEDIVDLE
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           FLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 471

[Close]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 466/470 (99.15%), Postives = 467/470 (99.36%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFI S
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDS 470

[Close]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 927 bits (2396), Expect = 0.0
Identity = 463/470 (98.51%), Postives = 466/470 (99.15%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKE LVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           LVTNAQFPDKIKSLKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 470

[Close]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 865 bits (2234), Expect = 1.11e-312
Identity = 433/472 (91.74%), Postives = 450/472 (95.34%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED--DEDADSGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE+  +ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 472

[Close]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 864 bits (2233), Expect = 1.57e-312
Identity = 433/472 (91.74%), Postives = 449/472 (95.13%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED--DEDADSGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE+  +ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 472

[Close]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 863 bits (2229), Expect = 6.16e-312
Identity = 432/471 (91.72%), Postives = 448/471 (95.12%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDE-DADSGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+K DA G G +L SGI  D DA SVEED+E D D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 470
           FLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI S
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 471

[Close]

BLAST of Csor.00g299410 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 657.5 bits (1695), Expect = 8.1e-189
Identity = 335/476 (70.38%), Postives = 385/476 (80.88%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLK-LSLNPTGC--SRRPKLFFISQTGTSKAL 63
           SS   +LA +ALLSA+T YC +K RRL+ LK LSLNP+    + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 123
           A RL  +  +N++AFDIVDP  +EPEDL KETLVLF+ASTW+ G PP + +FL +WL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRQLLKVLKVDAV--GTGADLYSGIDED----FDAESVEEDDEDADSGEED 243
           ++D+ F DW   +++VLK  +     G     G  ED    +D+   E++D DAD G   
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGG--- 244

Query: 244 IVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRW 303
           IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRW
Sbjct: 245 IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRW 304

Query: 304 TKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL 363
           TKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P 
Sbjct: 305 TKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPS 364

Query: 364 DIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHR 423
            IV  A+DLHK MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH 
Sbjct: 365 VIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHG 424

Query: 424 RRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIVS 471
           RRISTFLVTNAQFP+KI  +KP+TQLYVSVDAATK+SLKAIDRPLF DFWERFI S
Sbjct: 425 RRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDS 475

The following BLAST results are available for this feature:

BLAST of Csor.00g299410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXN5	1.1e-187	70.38	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
Q8H8N3	9.7e-171	65.47	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
Q2KHP8	6.6e-119	48.02	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopu...	[more]
Q08C92	4.0e-116	46.44	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio ...	[more]
Q9NV66	4.4e-115	45.63	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo s...	[more]

BLAST of Csor.00g299410 vs. NCBI nr


Match Name	Identity	Description
KAG6598440.1	100.00	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucu...	[more]
XP_023546392.1	99.15	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pep...	[more]
XP_022962153.1	99.15	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita mos...	[more]
XP_022997485.1	98.51	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita max...	[more]
XP_038884961.1	94.69	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa his...	[more]

BLAST of Csor.00g299410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HDZ5	0.0	99.15	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662...	[more]
A0A6J1K9S7	0.0	98.51	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 G...	[more]
A0A1S3BAY2	1.11e-312	91.74	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5A7VC83	1.57e-312	91.74	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LPA4	6.16e-312	91.72	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN...	[more]

BLAST of Csor.00g299410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75200.1	8.1e-189	70.38	flavodoxin family protein / radical SAM domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 47..60 score: 35.6 coord: 95..106 score: 39.97 coord: 158..177 score: 33.36 coord: 133..143 score: 47.16
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 323..467 e-value: 1.6E-16 score: 61.1
IPR007197	Radical SAM	SFLD	SFLDS00029	Radical_SAM	coord: 323..468 e-value: 5.4E-20 score: 67.0
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 e-value: 2.4E-25 score: 89.4
IPR008254	Flavodoxin/nitric oxide synthase	PROSITE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 22.03581
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 250..479 e-value: 1.5E-109 score: 368.1
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 32..203 e-value: 2.4E-39 score: 136.8
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 47..198
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..470
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..470
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 323..467 e-value: 1.05835E-5 score: 44.2466
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 324..457

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g299410.m01	Csor.00g299410.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0031591	wybutosine biosynthetic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0102521	tRNA-4-demethylwyosine synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding