Csor.00g285770 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g285770
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Kinetochore protein SPC25
Location	Csor_Chr10: 515824 .. 517725 (-)
RNA-Seq Expression	Csor.00g285770
Synteny	Csor.00g285770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCACTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGTGAGCCATCTATTCTCTTCAAATTATTCGGGAAAGAAGTGGATGCGATACCGTTATATTGTGGCGAGTTCAATAATGTAATCGGTAATAGAATTGTTTGTGAAGTCACCGATAGGGTTTTGAGATTGGTAGCGTACAAGACTCAAAAATGGATGTAATTCTAGGGGACTGAGTAGGTTTAGGTTCAGGAGTGGCCAAGTTGATTGATTTTTGAATTTTCAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGGTATGAGCTATGATTATGCTAATTTCCTCTAACGCTGCATGAGTAGAATACACTATTTCGTTGAATCGACTGAGGCTGCATGCTAATTATAAGGCTTATTTCCATTAGAATTGCGTGTTTTTGTAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGGTAAACTTAATTTATTTACGAGTGCTCACTCTCAAATCTGTTCTTGCTAGCCCTTAAAAACTGATTACTTTTCCTCCTTTCCAATGCCATCAAAAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGTATGATAATTTGAGTTAGTTGTTTTGTAACAACCTTTATATTTTTTGTGAATTCTTGAGATTAATTTCTCGACAACTTGTCTCGGGTTTTCAGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTGTAAGTTATGTGCACTTAATGTATGTTCATTTTTCATGTTCTTGATCTTCTGACAACCTTACTTTTTCAGTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGTGTGCGATGAATTTTGAACTTTTTGCTTGAAAGTGTGTTATTCACTGCACAAAAAACTCATTGGTACTTCATAGACATACTACCTGAAGCACCATGTTTTGTGCTTCTTGCAGTTTGTTAGATTGAACTTGTTCTGCATTAATAGTTTGTTATATTTTTGTTATTCAACAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAGGTATATTCAATTTAATGTTTGGTGTCTGGTGTTTATGATTTAAGATTCTCTGGTATATAATATTAAGAGAATGCTGTAACATCTGTGTTACAAGTTTGATTTTTTGTTTTATGCAAATTTCAAAATGTTATGCAAGAGAGAACTTGTAATGAAATGAGTCACGTATGGCAGGTCAAGAAATAA

mRNA sequence

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCACTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAGGTCAAGAAATAA

Coding sequence (CDS)

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCACTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAGGTCAAGAAATAA

Protein sequence

MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLGELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPRFKVKK

Homology

[Close]

BLAST of Csor.00g285770 vs. ExPASy Swiss-Prot
Match: Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.7e-54
Identity = 131/305 (42.95%), Postives = 196/305 (64.26%), Query Frame = 0


Query: 13  MESLRIVREKEIAVQQQKMESF-ADSFRKSLESITARSRENARSQGKLGELKARLREAED 72
           M SL ++ EK+I  Q+ K++SF A  FR+S+ S+  R++  A+SQ +L  LKA LREAED
Sbjct: 17  MASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKADLREAED 76

Query: 73  ELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSP 132
           ELVK LAVKTRK+A+++ + DS++ ++SRIE LR NLQ  K+++D+    IS+Q  ALS 
Sbjct: 77  ELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQLQALSK 136

Query: 133 YEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHAN 192
            +    +  + + +I EAISWYN  L FH+E GHGVKF+F  I+   P +E+SFT+ + N
Sbjct: 137 SKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSFTVHYGN 196

Query: 193 DTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTI 252
           D YTLLD +  L  I E++ ELN TN LF+FVR+MR +F ++    +   +  L Q ++ 
Sbjct: 197 DIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQLQQETSA 256

Query: 253 ISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPR 312
           IS SAP ++ S D + S+ E   S+VQ+      +++   G +  + +  S  + RR+ R
Sbjct: 257 ISASAPAISFSTDTNMSTPENKRSKVQV------NRRQKRGSESPLLAPVSTSATRRSSR 315

Query: 313 FKVKK 317
           FK KK
Sbjct: 317 FKGKK 315

[Close]

BLAST of Csor.00g285770 vs. ExPASy Swiss-Prot
Match: Q10430 (Kinetochore protein spc25 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spc25 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.5e-06
Identity = 49/215 (22.79%), Postives = 103/215 (47.91%), Query Frame = 0


Query: 28  QQKMESFADSFRKSLESITARSRENARSQ-GKLGELKARLREAEDELVKALAVK------ 87
           + K+ +F   F + L+    +   N      +L E+    ++AE  L +  A K      
Sbjct: 17  KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLSEINEAQKKAEKSLEQTEARKQNFTEL 76

Query: 88  TRKQAKRLALTD-SLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSPYEHKEPQE 147
             K+ +  A+T+  + + + +++ +    Q      D Y   IS +   L   E +  ++
Sbjct: 77  LEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEELDHYRAIISSKR-ELRAQEMEAKRK 136

Query: 148 RDCRNEIQEAISWYNRVLDFHIEGGHG--VKFSFKKINVDDPDKEYSFTIRHANDTYTLL 207
           +D  N  +  + ++   L   +EG H   ++F F  I+  D +K++SF I  A   Y ++
Sbjct: 137 QDSYNNPE--LKFWEDYLGLKMEGVHDEVIRFIFTNIDEKDWNKQFSFQINLAERDYKVV 196

Query: 208 DCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQE 233
            C+P L  +++L++++N T   ++F++ MR+ F+E
Sbjct: 197 HCHPPLPHVDDLVNKVNRTRDFYQFLKDMRKGFRE 228

[Close]

BLAST of Csor.00g285770 vs. NCBI nr
Match: KAG6589434.1 (Kinetochore protein SPC25-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590 bits (1522), Expect = 4.85e-212
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFKVKK 316
           GSAFSLRRTPRFKVKK
Sbjct: 301 GSAFSLRRTPRFKVKK 316

[Close]

BLAST of Csor.00g285770 vs. NCBI nr
Match: KAG7023114.1 (spc25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 583 bits (1503), Expect = 3.41e-209
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFK 313
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Csor.00g285770 vs. NCBI nr
Match: XP_022921533.1 (probable kinetochore protein SPC25 isoform X2 [Cucurbita moschata])

HSP 1 Score: 580 bits (1496), Expect = 4.44e-208
Identity = 309/316 (97.78%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS+
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQSI 300

Query: 301 GSAFSLRRTPRFKVKK 316
           GSAFSLRRTPRFKVKK
Sbjct: 301 GSAFSLRRTPRFKVKK 316

[Close]

BLAST of Csor.00g285770 vs. NCBI nr
Match: XP_023516085.1 (probable kinetochore protein SPC25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579 bits (1493), Expect = 1.27e-207
Identity = 309/316 (97.78%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRH NDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHGNDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEET+KPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETDKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFKVKK 316
           GSAFSLRRTPRFKVKK
Sbjct: 301 GSAFSLRRTPRFKVKK 316

[Close]

BLAST of Csor.00g285770 vs. NCBI nr
Match: XP_022921532.1 (probable kinetochore protein SPC25 isoform X1 [Cucurbita moschata])

HSP 1 Score: 576 bits (1484), Expect = 3.11e-206
Identity = 309/317 (97.48%), Postives = 313/317 (98.74%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TISRQSL ALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDP
Sbjct: 121 TISRQSLEALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFKVKK 316
           +GSAFSLRRTPRFKVKK
Sbjct: 301 IGSAFSLRRTPRFKVKK 317

[Close]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL
Match: A0A6J1E461 (Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 2.15e-208
Identity = 309/316 (97.78%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS+
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQSI 300

Query: 301 GSAFSLRRTPRFKVKK 316
           GSAFSLRRTPRFKVKK
Sbjct: 301 GSAFSLRRTPRFKVKK 316

[Close]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL
Match: A0A6J1E0R4 (Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 1.51e-206
Identity = 309/317 (97.48%), Postives = 313/317 (98.74%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESITARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TISRQSL ALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDP
Sbjct: 121 TISRQSLEALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFKVKK 316
           +GSAFSLRRTPRFKVKK
Sbjct: 301 IGSAFSLRRTPRFKVKK 317

[Close]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL
Match: A0A6J1JCY1 (Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1)

HSP 1 Score: 566 bits (1460), Expect = 6.84e-203
Identity = 304/317 (95.90%), Postives = 311/317 (98.11%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARS-QGKL 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKM+SFADSFRKSLESITARSRENAR  QGKL
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMQSFADSFRKSLESITARSRENARIIQGKL 60

Query: 61  GELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120
           GELKARLR+AEDEL KALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS
Sbjct: 61  GELKARLRKAEDELAKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120

Query: 121 TTISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TTISRQSLALSPYEHK+PQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP
Sbjct: 121 TTISRQSLALSPYEHKQPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRI+RRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIIRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSS DRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSSDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFKVKK 316
           LGSAFSLRRTPRFKVKK
Sbjct: 301 LGSAFSLRRTPRFKVKK 317

[Close]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL
Match: A0A6J1JH38 (Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 4.78e-201
Identity = 304/318 (95.60%), Postives = 311/318 (97.80%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARS-QGKL 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKM+SFADSFRKSLESITARSRENAR  QGKL
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMQSFADSFRKSLESITARSRENARIIQGKL 60

Query: 61  GELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120
           GELKARLR+AEDEL KALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS
Sbjct: 61  GELKARLRKAEDELAKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120

Query: 121 TTISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD 180
           TTISRQSL ALSPYEHK+PQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD
Sbjct: 121 TTISRQSLEALSPYEHKQPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD 180

Query: 181 PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGV 240
           PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRI+RRRFQEAAAEGV
Sbjct: 181 PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIIRRRFQEAAAEGV 240

Query: 241 GAQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQ 300
           GAQALSLHQGSTIISMSAPGLTSS DRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQ
Sbjct: 241 GAQALSLHQGSTIISMSAPGLTSSSDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQ 300

Query: 301 SLGSAFSLRRTPRFKVKK 316
           SLGSAFSLRRTPRFKVKK
Sbjct: 301 SLGSAFSLRRTPRFKVKK 318

[Close]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL
Match: A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 1.22e-175
Identity = 267/316 (84.49%), Postives = 291/316 (92.09%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60
           MENK E+S+  +MESLR+VREKEI VQQQKMESFADSFRKSLESIT RSRENA SQGKLG
Sbjct: 1   MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLA+TDSLA SKSRIEELRN LQDCKA+RDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
            IS+QSLALS  EHKE QE DCR+EIQEAISWYNRVLDFHIEGG GVKFSFKKIN++ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHA+DTYTLLDCNPSLQ I++LIHELN TNGLFKFVR+MR+RFQE AAEGVGA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQ STIIS+SAPGL+SS DRSESST+EID+QV+LEETNK SKK  SGKKPA+ S 
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300

Query: 301 GSAFSLRRTPRFKVKK 316
           GSAFSLRR+PRFKV+K
Sbjct: 301 GSAFSLRRSPRFKVRK 316

[Close]

BLAST of Csor.00g285770 vs. TAIR 10
Match: AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-55
Identity = 131/305 (42.95%), Postives = 196/305 (64.26%), Query Frame = 0


Query: 13  MESLRIVREKEIAVQQQKMESF-ADSFRKSLESITARSRENARSQGKLGELKARLREAED 72
           M SL ++ EK+I  Q+ K++SF A  FR+S+ S+  R++  A+SQ +L  LKA LREAED
Sbjct: 17  MASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKADLREAED 76

Query: 73  ELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSP 132
           ELVK LAVKTRK+A+++ + DS++ ++SRIE LR NLQ  K+++D+    IS+Q  ALS 
Sbjct: 77  ELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQLQALSK 136

Query: 133 YEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHAN 192
            +    +  + + +I EAISWYN  L FH+E GHGVKF+F  I+   P +E+SFT+ + N
Sbjct: 137 SKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSFTVHYGN 196

Query: 193 DTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTI 252
           D YTLLD +  L  I E++ ELN TN LF+FVR+MR +F ++    +   +  L Q ++ 
Sbjct: 197 DIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQLQQETSA 256

Query: 253 ISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPR 312
           IS SAP ++ S D + S+ E   S+VQ+      +++   G +  + +  S  + RR+ R
Sbjct: 257 ISASAPAISFSTDTNMSTPENKRSKVQV------NRRQKRGSESPLLAPVSTSATRRSSR 315

Query: 313 FKVKK 317
           FK KK
Sbjct: 317 FKGKK 315

The following BLAST results are available for this feature:

BLAST of Csor.00g285770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK9	1.7e-54	42.95	Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ...	[more]
Q10430	6.5e-06	22.79	Kinetochore protein spc25 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)...	[more]

BLAST of Csor.00g285770 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6589434.1	4.85e-212	100.00	Kinetochore protein SPC25-like protein, partial [Cucurbita argyrosperma subsp. s...	[more]
KAG7023114.1	3.41e-209	99.68	spc25, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022921533.1	4.44e-208	97.78	probable kinetochore protein SPC25 isoform X2 [Cucurbita moschata]	[more]
XP_023516085.1	1.27e-207	97.78	probable kinetochore protein SPC25 [Cucurbita pepo subsp. pepo]	[more]
XP_022921532.1	3.11e-206	97.48	probable kinetochore protein SPC25 isoform X1 [Cucurbita moschata]	[more]

BLAST of Csor.00g285770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E461	2.15e-208	97.78	Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=...	[more]
A0A6J1E0R4	1.51e-206	97.48	Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=...	[more]
A0A6J1JCY1	6.84e-203	95.90	Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1	[more]
A0A6J1JH38	4.78e-201	95.60	Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1	[more]
A0A6J1BYG5	1.22e-175	84.49	Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV...	[more]

BLAST of Csor.00g285770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48210.1	1.2e-55	42.95	CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 94..121
None	No IPR available	COILS	Coil	Coil	coord: 52..86
None	No IPR available	GENE3D	3.30.457.50	Chromosome segregation protein Spc25	coord: 139..236 e-value: 1.2E-26 score: 94.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..305
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..316
None	No IPR available	PANTHER	PTHR14281:SF3	SUBFAMILY NOT NAMED	coord: 1..314
IPR013255	Chromosome segregation protein Spc25, C-terminal	PFAM	PF08234	Spindle_Spc25	coord: 163..231 e-value: 5.4E-23 score: 81.0
IPR045143	Kinetochore protein Spc25	PANTHER	PTHR14281	KINETOCHORE PROTEIN SPC25-RELATED	coord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g285770.m01	Csor.00g285770.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0051301	cell division
biological_process	GO:0007059	chromosome segregation
cellular_component	GO:0031262	Ndc80 complex
cellular_component	GO:0005634	nucleus