Csor.00g278540 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g278540
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr19: 9659993 .. 9662168 (-)
RNA-Seq ExpressionCsor.00g278540
SyntenyCsor.00g278540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTTTTCCAGCTGATTTCATGTTTCTTTTTATGCTTTCTCAACTTTCTGGCTGTTGTTGATTCCACCCATTTTAATGCAACAGTAGCATTAGACGGAACGGGAAACTTCAAAAGCATTACTGATGCAATTGCAGCCGCTCCAAACAACTCCAATACACGATTTTACATTCGTGTTTCTCCTGGCATTTACCATGAACATCTTGAAATTGCATCAGCCAAGAAGTTCATTGCTTTGATTGGCGAGAATGAGTTTACAACCATTATCGTCGACAATAGAAGTAATGCCGGTGGATTCAGAACCTCTGAATCCGCAACCATGGGTAATTACTCTTATTCTTTATTTTTATGAAATAATTTGTTTGAGCAAAATAAATCTTACCTCTGATAAAATGGGGTAAGGTGTTAATATTTAGCTAAAAAACTTGAATCCATATGACAATTATTTTCTCCTTTTGAATTAGGCTTTCTTTTGGAGAATCGTATGTAGAATAATATTGTTTGAATCCTTACTAATTTCTTTATTCTTATTTGATGCAGTGGTCAAAGGTAACAACTTTATGGCCCAACATTTGAGTTTCGAGAATTCTGCAGACCATCAAGACGGCCAAGCAGTTGCTGTTCTTGACGATGCCAAATACACGGTTTATTACAAATGCAGATTCCTGAGTTTTCAAGACACCCTATATTCGAAGGGAGAATATCAATTCTTTAAGGGGTGCGACATCTATGGAACGGTGGATTTTATTTTTGGCAATGGACTTGCTATGTTCCAAGACTGTAACGTATATGCTCGGTTACCGAGCAATGACTCTACAGTCACTGCACAGTCCAAGAATCAATGGACCCAAAGTGGCTTCTCCTTTCAGAACTGTACAGTCACAGTTTCTCCGGAGATAGCTTCCTGTAAGGCCTTAGTTAAAATCTATCTTGGTCGGCCATGGAAACCATATTCAACAGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAAGCACAGGGATGGATGTTGTGGCCAGGGGTACCAGTAAACAACCTCTTTTATGCTGAGTACAATAATAGAGGTCCTGGTGCTAACACAACACACCGAGTTAATTGGCCAGGTTTTCATGTAATTGATAGACAATTAGCTAAAAGTTTTACGGTAGAGAACTTTATTAATGGGACTTATTGGTTGCCCGAAACAAATGTACCCTATACAAGTGGACTTTATTCTTAATGAAATTGCCTCATCAACATTGATAAATATCCATATGCATGTAAAAGTTTTATTGTAGAGAACTTTATTTTTTTTAATGAGCACATCTCAGGTTTAATGAAAAGGTTAAATAAAAAATATTATTTAATTAAAAAAATATTTAAGACTTACCTTTTACCTTAAGATTCCTATTTGACCTTATGAATTGTTATATACATCCATTCCTAATTAAATGTGCCCGAACTAATTTCCTAATTAAATGTACATGAACTAATTTCCTAATTAAATGTACATGAACTAATTTCCACAACAAGTTGGGAAATTCATTAGTTTTGCACTATAAATTTCTACTATGTCTAAATAGGATTATTGCCAAAGATGAAGAACACGTCTTCTTCCCTCACATTTCTGGGTGCTTTTACCCTTCTTTTACTATCCATTTTCAACCCTTCCATGTCAGCCAAGGTCGAAGAGACTAGAAGCACCTTTGTACATGATACTTGCAACAAATTTCAAATTGAGCAATTGTATAAGTTGTGCATTCCTGAGATTGATTCAGATCCTCGTGAAGATTTGAAGTCAAATTTGACAGGTATTTTAATTATATTTGTGAATCATAGCTTCAGTAATTTCAACGACAACATTGCGTTCCTTCAAAGAGAGATAAAGTCGGGAAAGTTAAATGGTGAAACAAAAGTGATGTATAATGGGTGTTTGGAAGATTTTGAAAGAGGAAATGGTGATTTGCAAGAAACAATGCATATACTACTTACCCAAACGGGGATAGACGCGTATAATTTGCCAACAATCAATATTCCTAACTATGTAAGTGAATGCGTTGATTTTTATGAAGGTGACCCTATACCACCCGAGTGGCAGTCTCGCTACAATACTTCATGGAACTTATTGACTCTTATTGGAGTAACGTCTAATCTCATGAAATGTAATCATGACATTGCATGTATCCCTTAA

mRNA sequence

ATGGCGTTTTTCCAGCTGATTTCATGTTTCTTTTTATGCTTTCTCAACTTTCTGGCTGTTGTTGATTCCACCCATTTTAATGCAACAGTAGCATTAGACGGAACGGGAAACTTCAAAAGCATTACTGATGCAATTGCAGCCGCTCCAAACAACTCCAATACACGATTTTACATTCGTGTTTCTCCTGGCATTTACCATGAACATCTTGAAATTGCATCAGCCAAGAAGTTCATTGCTTTGATTGGCGAGAATGAGTTTACAACCATTATCGTCGACAATAGAAGTAATGCCGGTGGATTCAGAACCTCTGAATCCGCAACCATGGTGGTCAAAGGTAACAACTTTATGGCCCAACATTTGAGTTTCGAGAATTCTGCAGACCATCAAGACGGCCAAGCAGTTGCTGTTCTTGACGATGCCAAATACACGGTTTATTACAAATGCAGATTCCTGAGTTTTCAAGACACCCTATATTCGAAGGGAGAATATCAATTCTTTAAGGGGTGCGACATCTATGGAACGGTGGATTTTATTTTTGGCAATGGACTTGCTATGTTCCAAGACTGTAACGTATATGCTCGGTTACCGAGCAATGACTCTACAGTCACTGCACAGTCCAAGAATCAATGGACCCAAAGTGGCTTCTCCTTTCAGAACTGTACAGTCACAGTTTCTCCGGAGATAGCTTCCTGTAAGGCCTTAGTTAAAATCTATCTTGGTCGGCCATGGAAACCATATTCAACAGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAAGCACAGGGATGGATGTTGTGGCCAGGGGTACCAGTAAACAACCTCTTTTATGCTGAGTACAATAATAGAGGTCCTGGTGCTAACACAACACACCGAGTTAATTGGCCAGGATTATTGCCAAAGATGAAGAACACGTCTTCTTCCCTCACATTTCTGGGTGCTTTTACCCTTCTTTTACTATCCATTTTCAACCCTTCCATGTCAGCCAAGGTCGAAGAGACTAGAAGCACCTTTGTACATGATACTTGCAACAAATTTCAAATTGAGCAATTGTATAAGTTGTGCATTCCTGAGATTGATTCAGATCCTCGTGAAGATTTGAAGTCAAATTTGACAGGTATTTTAATTATATTTGTGAATCATAGCTTCAGTAATTTCAACGACAACATTGCGTTCCTTCAAAGAGAGATAAAGTCGGGAAAGTTAAATGGTGAAACAAAAGTGATGTATAATGGGTGTTTGGAAGATTTTGAAAGAGGAAATGGTGATTTGCAAGAAACAATGCATATACTACTTACCCAAACGGGGATAGACGCGTATAATTTGCCAACAATCAATATTCCTAACTATGTAAGTGAATGCGTTGATTTTTATGAAGGTGACCCTATACCACCCGAGTGGCAGTCTCGCTACAATACTTCATGGAACTTATTGACTCTTATTGGAGTAACGTCTAATCTCATGAAATGTAATCATGACATTGCATGTATCCCTTAA

Coding sequence (CDS)

ATGGCGTTTTTCCAGCTGATTTCATGTTTCTTTTTATGCTTTCTCAACTTTCTGGCTGTTGTTGATTCCACCCATTTTAATGCAACAGTAGCATTAGACGGAACGGGAAACTTCAAAAGCATTACTGATGCAATTGCAGCCGCTCCAAACAACTCCAATACACGATTTTACATTCGTGTTTCTCCTGGCATTTACCATGAACATCTTGAAATTGCATCAGCCAAGAAGTTCATTGCTTTGATTGGCGAGAATGAGTTTACAACCATTATCGTCGACAATAGAAGTAATGCCGGTGGATTCAGAACCTCTGAATCCGCAACCATGGTGGTCAAAGGTAACAACTTTATGGCCCAACATTTGAGTTTCGAGAATTCTGCAGACCATCAAGACGGCCAAGCAGTTGCTGTTCTTGACGATGCCAAATACACGGTTTATTACAAATGCAGATTCCTGAGTTTTCAAGACACCCTATATTCGAAGGGAGAATATCAATTCTTTAAGGGGTGCGACATCTATGGAACGGTGGATTTTATTTTTGGCAATGGACTTGCTATGTTCCAAGACTGTAACGTATATGCTCGGTTACCGAGCAATGACTCTACAGTCACTGCACAGTCCAAGAATCAATGGACCCAAAGTGGCTTCTCCTTTCAGAACTGTACAGTCACAGTTTCTCCGGAGATAGCTTCCTGTAAGGCCTTAGTTAAAATCTATCTTGGTCGGCCATGGAAACCATATTCAACAGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAAGCACAGGGATGGATGTTGTGGCCAGGGGTACCAGTAAACAACCTCTTTTATGCTGAGTACAATAATAGAGGTCCTGGTGCTAACACAACACACCGAGTTAATTGGCCAGGATTATTGCCAAAGATGAAGAACACGTCTTCTTCCCTCACATTTCTGGGTGCTTTTACCCTTCTTTTACTATCCATTTTCAACCCTTCCATGTCAGCCAAGGTCGAAGAGACTAGAAGCACCTTTGTACATGATACTTGCAACAAATTTCAAATTGAGCAATTGTATAAGTTGTGCATTCCTGAGATTGATTCAGATCCTCGTGAAGATTTGAAGTCAAATTTGACAGGTATTTTAATTATATTTGTGAATCATAGCTTCAGTAATTTCAACGACAACATTGCGTTCCTTCAAAGAGAGATAAAGTCGGGAAAGTTAAATGGTGAAACAAAAGTGATGTATAATGGGTGTTTGGAAGATTTTGAAAGAGGAAATGGTGATTTGCAAGAAACAATGCATATACTACTTACCCAAACGGGGATAGACGCGTATAATTTGCCAACAATCAATATTCCTAACTATGTAAGTGAATGCGTTGATTTTTATGAAGGTGACCCTATACCACCCGAGTGGCAGTCTCGCTACAATACTTCATGGAACTTATTGACTCTTATTGGAGTAACGTCTAATCTCATGAAATGTAATCATGACATTGCATGTATCCCTTAA

Protein sequence

MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGLLPKMKNTSSSLTFLGAFTLLLLSIFNPSMSAKVEETRSTFVHDTCNKFQIEQLYKLCIPEIDSDPREDLKSNLTGILIIFVNHSFSNFNDNIAFLQREIKSGKLNGETKVMYNGCLEDFERGNGDLQETMHILLTQTGIDAYNLPTINIPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLTLIGVTSNLMKCNHDIACIP
Homology
BLAST of Csor.00g278540 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.5e-69
Identity = 131/295 (44.41%), Postives = 191/295 (64.75%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPNNSNTR---FYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV+ DGTGNF +IT A+AAAPNN++     F I V+ GIY E++ IA  K+++ +IG+  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T++  NRS   G+ T  SAT  V   NF+A +++F N+A  +  QAVA+   A ++++
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYAR--LPSNDSTVT 208
           Y C F ++QDTLY+    QF++ CD+YGTVDFIFGN   +FQ+CN+Y R  +P+  + +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ ++   Q +G S QNCT+  + ++ S    VK YLGRPWK YS  V+M+S++D  V+ 
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPGLLPKMKNTSSSLTFLGAF 317
            GW  W G   ++ L+YAEYNN GPG+NTT+RV WPG        +++ T  G F
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLF 560

BLAST of Csor.00g278540 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.8e-64
Identity = 128/302 (42.38%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 19  AVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASAKKFI 78
           A V  T++N +VA+DGTGNF +I DA+ AAPN S TRF I +  G Y E++E+   K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLD 138
             IG+    T+I  NRS   G+ T ++ T+ VKG  ++A+ +SF NSA     QAVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 DAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSN 198
            + ++ +Y+C F  +QDTLY     QF++ CDIYGT+DFIFGN   +FQ+ ++YAR P+ 
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 DSTV--TAQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESF 258
              +  TAQS+NQ  Q +G S  NC +  +P++   K   K YLGRPW+ YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQAQGWMLW-PGVPVNNLFYAEYNNRGPGANTTHRVNWPGLLPKMKNTSSSLTFLG 317
           +DD +   GW+       +  L+Y EY N GPGAN   RV WPG       T ++   +G
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

BLAST of Csor.00g278540 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 1.4e-63
Identity = 122/277 (44.04%), Postives = 178/277 (64.26%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPNNSNTR---FYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV  +GTGNF +IT+A+ +APN ++     F I V+ G+Y E++ IA  K+++ +IG+  
Sbjct: 260 TVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGI 319

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T++  NR+   G+ T  SAT  V   NF+A +++F N+A  +  QAVA+   A  +++
Sbjct: 320 NRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIF 379

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSND--STVT 208
           Y C F ++QDTLY+    QF++ CDIYGTVDFIFGN   +FQDCN+Y R P  +  + +T
Sbjct: 380 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAIT 439

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ +    Q +G S  NCT+  + ++ S    VK YLGRPWK YS  VFM+S++D+ V+ 
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPG 299
            GW  W G   ++ L+YAEYNN G G++TT RV WPG
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPG 536

BLAST of Csor.00g278540 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 126/282 (44.68%), Postives = 176/282 (62.41%), Query Frame = 0

Query: 21  VDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASAKKFIAL 80
           VD T  N  VA +GTGN+ +I +AI+AAPN+S TRF I +  G Y E++EI   K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDA 140
           IG+    T+I  NRS A G+    SAT+ V+G+ F+A+ LSF N A  +  QAVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 KYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSNDS 200
             + YY+C F S+QDT+Y     QF++ CDIYGTVDFIFG+   +FQ+C++YAR P+ + 
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 TV--TAQSK-NQWTQSGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLD 260
            +  TAQ + N    +G S  +  +  +P++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQAQGWMLW-PGVPVNNLFYAEYNNRGPGANTTHRVNWPG 299
           D V   GW+ W     +  L+Y EY N GPG+N T+RV WPG
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPG 482

BLAST of Csor.00g278540 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 1.2e-62
Identity = 120/277 (43.32%), Postives = 174/277 (62.82%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPN---NSNTRFYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV  +GTGNF +I  AIAAAPN    SN  F I V+ G+Y E++E+   K+++ +IG+  
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T+I  NRS   G+ T  SAT ++ G NF+  +++  N+A    GQAVA+      +V+
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLP--SNDSTVT 208
           Y C F ++QDTLY+    QF++ CD+YGTVDFIFGN   + Q+CN+Y R P     + VT
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ +    Q +G +   CT+  + ++A+    VK YLGRPWK YS  V M++++D  ++ 
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPG 299
            GW  W G   ++ L+YAEYNN GPG++TT+RV WPG
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPG 523

BLAST of Csor.00g278540 vs. NCBI nr
Match: KAG6572151.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGLL 300
           RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGLL
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGLL 300

Query: 301 PKMKNTSSSLTFLGAFTLLLLSIFNPSMSAKVEETRSTFVHDTCNKFQIEQLYKLCIPEI 360
           PKMKNTSSSLTFLGAFTLLLLSIFNPSMSAKVEETRSTFVHDTCNKFQIEQLYKLCIPEI
Sbjct: 301 PKMKNTSSSLTFLGAFTLLLLSIFNPSMSAKVEETRSTFVHDTCNKFQIEQLYKLCIPEI 360

Query: 361 DSDPREDLKSNLTGILIIFVNHSFSNFNDNIAFLQREIKSGKLNGETKVMYNGCLEDFER 420
           DSDPREDLKSNLTGILIIFVNHSFSNFNDNIAFLQREIKSGKLNGETKVMYNGCLEDFER
Sbjct: 361 DSDPREDLKSNLTGILIIFVNHSFSNFNDNIAFLQREIKSGKLNGETKVMYNGCLEDFER 420

Query: 421 GNGDLQETMHILLTQTGIDAYNLPTINIPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLT 480
           GNGDLQETMHILLTQTGIDAYNLPTINIPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLT
Sbjct: 421 GNGDLQETMHILLTQTGIDAYNLPTINIPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLT 480

Query: 481 LIGVTSNLMKCNHDIACIP 499
           LIGVTSNLMKCNHDIACIP
Sbjct: 481 LIGVTSNLMKCNHDIACIP 499

BLAST of Csor.00g278540 vs. NCBI nr
Match: XP_022952379.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X2 [Cucurbita moschata])

HSP 1 Score: 605 bits (1560), Expect = 1.48e-214
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQ ISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSK QWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298
           RPWKPYSTVVFMESFLDDNVQ QGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298

BLAST of Csor.00g278540 vs. NCBI nr
Match: XP_022952377.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 605 bits (1560), Expect = 1.99e-214
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQ ISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSK QWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298
           RPWKPYSTVVFMESFLDDNVQ QGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298

BLAST of Csor.00g278540 vs. NCBI nr
Match: XP_023554541.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 603 bits (1554), Expect = 1.63e-213
Identity = 294/298 (98.66%), Postives = 294/298 (98.66%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQLISCFFLCF NFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQLISCFFLCFFNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           S GIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SSGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSK QWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298
           RPWKPYSTVVFMESFLDDNVQ QGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298

BLAST of Csor.00g278540 vs. NCBI nr
Match: KAG6572150.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494 bits (1272), Expect = 3.95e-166
Identity = 298/639 (46.64%), Postives = 362/639 (56.65%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MA F  ++CFF+CF+NFLA VDS  FNA VALDG+G++K I+DAIAAAP+NS TRFYI V
Sbjct: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           +PGIY EH++I S KKFIALIG+N FTTIIVDNRSN  GF TS+SATM VKGNNF+A+HL
Sbjct: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATMEVKGNNFIARHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSA  Q+ QAVAVLD+AKYT YYKCRFL FQDTLY KG   FFK CDIYGTVDFIFG
Sbjct: 121 SFENSAGPQNHQAVAVLDNAKYTAYYKCRFLGFQDTLYLKGTNNFFKECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240
           +GLAMFQDCN+YARL  N+  VTAQSK +    SGF FQNCTVTVSPEI + KA +KIYL
Sbjct: 181 HGLAMFQDCNIYARLSRNEIVVTAQSKEKSMVHSGFCFQNCTVTVSPEIRANKAKIKIYL 240

Query: 241 GRPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGL 300
           GRPW+ YS+VVFMESFLD+NVQ +GW  W GVP+N+LFYAE+NN G GANT HRVNWP L
Sbjct: 241 GRPWRQYSSVVFMESFLDNNVQPEGWRFWQGVPINHLFYAEFNNGGLGANTKHRVNWPAL 300

Query: 301 ----------------------------LP-------KMKNTSSSLTFLG--AFTLLLLS 360
                                        P       ++ +T   +  +G  AFT +++ 
Sbjct: 301 DGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTTIIVD 360

Query: 361 I------FNPSMSAKVEETRSTFV------------------------------------ 420
                  F+ S SA +E   + FV                                    
Sbjct: 361 NRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHQAVAVLDQADYTAYYRCRFLG 420

Query: 421 -HDTCNKFQIEQLYKLC-------------------------------IPEIDSDPREDL 480
             DT        L K C                               +    S  +  L
Sbjct: 421 FQDTLYLKGHNNLLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKDQPML 480

Query: 481 KSNLT------------GI----LIIFVNHSFSNFNDNI---AFLQREIKSGKLNGETKV 497
           KS  +            G+    + I+++  +  ++  +   +FL   ++         V
Sbjct: 481 KSGFSFQNCIVTVSSEIGVDEAKIKIYLDRPWRQYSSVVFMESFLDDNVQPKGWMFWQGV 540

BLAST of Csor.00g278540 vs. ExPASy TrEMBL
Match: A0A6J1GLJ0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 7.15e-215
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQ ISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSK QWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298
           RPWKPYSTVVFMESFLDDNVQ QGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298

BLAST of Csor.00g278540 vs. ExPASy TrEMBL
Match: A0A6J1GK92 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 9.62e-215
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAFFQ ISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240
           NGLAMFQDCNVYARLPSNDSTVTAQSK QWTQSGFSFQNCTVTVSPEIASCKALVKIYLG
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQWTQSGFSFQNCTVTVSPEIASCKALVKIYLG 240

Query: 241 RPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298
           RPWKPYSTVVFMESFLDDNVQ QGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG
Sbjct: 241 RPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 298

BLAST of Csor.00g278540 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 471 bits (1212), Expect = 7.42e-162
Identity = 231/298 (77.52%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAF QL +C F  F N LA+VDS  FNA V+LDG+G+FKSI++AIAAAPNNSNTRFYIRV
Sbjct: 1   MAFLQLAACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           +PGIY  HLEI  AKKFIALIG++  TTIIVDNRS+  GF+TSESAT+VV G+NFMAQHL
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSA+ Q+GQAVAVLD AK++VYY CRF+SFQDTLYSKGE+QFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQW-TQSGFSFQNCTVTVSPEIASCKALVKIYL 240
           NGLA+FQDCN+YARLP ++ TVTAQSK Q  +QSGFSFQNCTVTVSPEI + K  VKIYL
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIYL 240

Query: 241 GRPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWP 297
           GRPW+ YSTVVFMESFLDDNVQ QGW+ W GVP+NNLFYAE+NNRGPGANTTHRVNWP
Sbjct: 241 GRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWP 298

BLAST of Csor.00g278540 vs. ExPASy TrEMBL
Match: A0A6J1HZD2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468060 PE=4 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.41e-160
Identity = 230/297 (77.44%), Postives = 260/297 (87.54%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           M F QL +C F  F+N LA+VD   FNA V+LDG+G+FKSI++AIAAAP+NSN RFYI V
Sbjct: 1   MTFLQLAACLFFFFVNVLALVDPIRFNAVVSLDGSGDFKSISEAIAAAPDNSNARFYIHV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           +PGIY  HLEI  AKKFIALIG+N  TTIIVDNRS+  GF+TSESAT+VV G+NFMAQHL
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESATVVVNGDNFMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSA+ Q+GQAVAVLD AK+TVYY CRFLSFQDTLYSKGE QFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHTVYYDCRFLSFQDTLYSKGERQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQW-TQSGFSFQNCTVTVSPEIASCKALVKIYL 240
           NGLA+FQDCN+YARLP ++ TVTAQSK Q  +QSGFSFQNCTVTVSPEIA+ K  VKIYL
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSISQSGFSFQNCTVTVSPEIAANKTKVKIYL 240

Query: 241 GRPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNW 296
           GRPW+PYSTVVFMESFLDDNVQ QGW+LW GVP+NNLFYAE+NNRGPGA+TTHRVNW
Sbjct: 241 GRPWRPYSTVVFMESFLDDNVQLQGWILWEGVPINNLFYAEFNNRGPGADTTHRVNW 297

BLAST of Csor.00g278540 vs. ExPASy TrEMBL
Match: A0A6J1GMM0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1)

HSP 1 Score: 458 bits (1179), Expect = 7.50e-157
Identity = 223/297 (75.08%), Postives = 255/297 (85.86%), Query Frame = 0

Query: 1   MAFFQLISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60
           MAF    +C F  F N LA VD   FNA V+LDG+G+FKSI++AIAAAP+NSNTRFYIRV
Sbjct: 1   MAFLSFAACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120
           SPG Y  HLEI   KKFIALIG+N  TTIIVDNRS+  GF T++SAT+VV G+++MAQHL
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQHL 120

Query: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180
           SFENSA+HQ+GQAVAVL+ +K+T YYKCRFLSFQDTLY KG YQFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKNQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240
           NGLAMFQDCN+YARL SN+ TVTAQSK Q W +SGFSFQNCTVTVSP+IAS K  VKI+L
Sbjct: 181 NGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIFL 240

Query: 241 GRPWKPYSTVVFMESFLDDNVQAQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNW 296
           GRPW+ YSTVVFMESFLDDNVQ +GW+LW GVP+NNLFYAE+NNRGPGA+TTHRVNW
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNW 297

BLAST of Csor.00g278540 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 262.7 bits (670), Expect = 6.0e-70
Identity = 131/295 (44.41%), Postives = 191/295 (64.75%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPNNSNTR---FYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV+ DGTGNF +IT A+AAAPNN++     F I V+ GIY E++ IA  K+++ +IG+  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T++  NRS   G+ T  SAT  V   NF+A +++F N+A  +  QAVA+   A ++++
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYAR--LPSNDSTVT 208
           Y C F ++QDTLY+    QF++ CD+YGTVDFIFGN   +FQ+CN+Y R  +P+  + +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ ++   Q +G S QNCT+  + ++ S    VK YLGRPWK YS  V+M+S++D  V+ 
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPGLLPKMKNTSSSLTFLGAF 317
            GW  W G   ++ L+YAEYNN GPG+NTT+RV WPG        +++ T  G F
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLF 560

BLAST of Csor.00g278540 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-65
Identity = 128/302 (42.38%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 19  AVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASAKKFI 78
           A V  T++N +VA+DGTGNF +I DA+ AAPN S TRF I +  G Y E++E+   K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLD 138
             IG+    T+I  NRS   G+ T ++ T+ VKG  ++A+ +SF NSA     QAVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 DAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSN 198
            + ++ +Y+C F  +QDTLY     QF++ CDIYGT+DFIFGN   +FQ+ ++YAR P+ 
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 DSTV--TAQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESF 258
              +  TAQS+NQ  Q +G S  NC +  +P++   K   K YLGRPW+ YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQAQGWMLW-PGVPVNNLFYAEYNNRGPGANTTHRVNWPGLLPKMKNTSSSLTFLG 317
           +DD +   GW+       +  L+Y EY N GPGAN   RV WPG       T ++   +G
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

BLAST of Csor.00g278540 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 245.4 bits (625), Expect = 1.0e-64
Identity = 122/277 (44.04%), Postives = 178/277 (64.26%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPNNSNTR---FYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV  +GTGNF +IT+A+ +APN ++     F I V+ G+Y E++ IA  K+++ +IG+  
Sbjct: 260 TVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGI 319

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T++  NR+   G+ T  SAT  V   NF+A +++F N+A  +  QAVA+   A  +++
Sbjct: 320 NRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIF 379

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSND--STVT 208
           Y C F ++QDTLY+    QF++ CDIYGTVDFIFGN   +FQDCN+Y R P  +  + +T
Sbjct: 380 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAIT 439

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ +    Q +G S  NCT+  + ++ S    VK YLGRPWK YS  VFM+S++D+ V+ 
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPG 299
            GW  W G   ++ L+YAEYNN G G++TT RV WPG
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPG 536

BLAST of Csor.00g278540 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-64
Identity = 126/282 (44.68%), Postives = 176/282 (62.41%), Query Frame = 0

Query: 21  VDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASAKKFIAL 80
           VD T  N  VA +GTGN+ +I +AI+AAPN+S TRF I +  G Y E++EI   K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDA 140
           IG+    T+I  NRS A G+    SAT+ V+G+ F+A+ LSF N A  +  QAVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 KYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLPSNDS 200
             + YY+C F S+QDT+Y     QF++ CDIYGTVDFIFG+   +FQ+C++YAR P+ + 
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 TV--TAQSK-NQWTQSGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLD 260
            +  TAQ + N    +G S  +  +  +P++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQAQGWMLW-PGVPVNNLFYAEYNNRGPGANTTHRVNWPG 299
           D V   GW+ W     +  L+Y EY N GPG+N T+RV WPG
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPG 482

BLAST of Csor.00g278540 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 242.3 bits (617), Expect = 8.4e-64
Identity = 120/277 (43.32%), Postives = 174/277 (62.82%), Query Frame = 0

Query: 29  TVALDGTGNFKSITDAIAAAPN---NSNTRFYIRVSPGIYHEHLEIASAKKFIALIGENE 88
           TV  +GTGNF +I  AIAAAPN    SN  F I V+ G+Y E++E+   K+++ +IG+  
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 89  FTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAVAVLDDAKYTVY 148
             T+I  NRS   G+ T  SAT ++ G NF+  +++  N+A    GQAVA+      +V+
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 149 YKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYARLP--SNDSTVT 208
           Y C F ++QDTLY+    QF++ CD+YGTVDFIFGN   + Q+CN+Y R P     + VT
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 209 AQSKNQWTQ-SGFSFQNCTVTVSPEIASCKALVKIYLGRPWKPYSTVVFMESFLDDNVQA 268
           AQ +    Q +G +   CT+  + ++A+    VK YLGRPWK YS  V M++++D  ++ 
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 269 QGWMLWPG-VPVNNLFYAEYNNRGPGANTTHRVNWPG 299
            GW  W G   ++ L+YAEYNN GPG++TT+RV WPG
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPG 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRX48.5e-6944.41Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O814152.8e-6442.38Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK71.4e-6344.04Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O813013.1e-6344.68Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O222561.2e-6243.32Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
KAG6572151.10.0100.00putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
XP_022952379.11.48e-21498.99probable pectinesterase/pectinesterase inhibitor 7 isoform X2 [Cucurbita moschat... [more]
XP_022952377.11.99e-21498.99probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita moschat... [more]
XP_023554541.11.63e-21398.66probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita pepo su... [more]
KAG6572150.13.95e-16646.64putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
A0A6J1GLJ07.15e-21598.99Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1[more]
A0A6J1GK929.62e-21598.99Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1[more]
A0A6J1GM547.42e-16277.52Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1[more]
A0A6J1HZD21.41e-16077.44Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468060 PE=4 SV=1[more]
A0A6J1GMM07.50e-15775.08Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.16.0e-7044.41Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.12.0e-6542.38Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.11.0e-6444.04Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02320.12.2e-6444.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.18.4e-6443.32Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 336..489
e-value: 2.9E-6
score: 29.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 338..479
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 22..316
e-value: 6.7E-99
score: 333.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 27..306
e-value: 4.4E-94
score: 314.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 315..443
e-value: 5.7E-12
score: 43.3
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 26..299
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 26..299
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 171..180
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 26..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g278540.m01Csor.00g278540.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity