Csor.00g266610 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g266610
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr17: 8909520 .. 8912265 (+)
RNA-Seq ExpressionCsor.00g266610
SyntenyCsor.00g266610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACGGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTATTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACAATGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGACTGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTCAGTTCTCTGTTTCACTCTTTTTTTTAATAAATAAATAATTAATTACTATCATTAAAATGCTGAAACCACCTGACGACCATCAGATCTTGCCTCTGAACTAATTTATTTATTTATTAATCATAATTTTGGATCTGGTCTAATATTTTAAAATTAGAAAACGTTTCTTAATTTAATGTGTTTTTTTTTTTAATTTTAGAAAATAAATAGGTATAGAATTGAAACTTAAAAAAGAAAATCAAAATTTAAAATAGAAAAGGGAAATTAGTAGATAATAGTTGAATATAAAATAGACTAAACTAATAAAAAAAAAAAACATTTTTAAAATTTTAAAAGTTTAATAATACAAATTTTTTAAAAAAATTAATATTTTATTTAAAAAATATCTTTTAATTTTTATAAATTCCCGTAAACTTTCAAAAGTTTCATTATATAATTTTAAAATGTCATTAAATATTTTAAATTTTTTAAAAAATAATACTGTTAATATCCATTTAAAAATACTAATGAACTTCTAAAAGTAACACGAGATAATAGACTGTTTTCGTTTATATATTGACGATAATTTTTTTTTGAATTGTTTTATTTATAAAAATTAAGGGGTTAATGTTACAGGGTTTTCAAATAAAAATTTAAATTAAAAGGTATTAGTAAAAAAAATTTAATTTAAAAATATTATTGCAAAATTGATAATTTTTAAAACATTTAAAATTATTATTGAATTTTTTGAAAATTTAGGTATGTTTTTTTATTAATTATTTTGAAAGTGGCAGAAGGGTAGGGGAAGGCGAGTACAGTGGGCCCAGTGGGCCCAGTGTGGCCCAAAAGCCGCTAATATGGGCGAACCGCATGTGGGTTGGATAAGATTGCCAATCTCCGGAAAAAAGGGCCGTTGCCTTTTTCAATTAATTTTTCAATTTATTCTATAAATTTATAAATGTTTTTTAAATCTACTTTTTTATTTGGTCTTATAATTTTCTGTTGACAGTTTTAGTTTTAATATTTGGTATTTGATGTTAAATTTTTTTAATTTTTTCTTTCGAGAATAATAACATATGTTAGTTACTATGTTCAGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCCTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCGTTTATTGGGCAGTTGGTTGAGCCAGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAATTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGA

mRNA sequence

ATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACGGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTATTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACAATGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGACTGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCCTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCGTTTATTGGGCAGTTGGTTGAGCCAGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAATTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGA

Coding sequence (CDS)

ATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACGGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTATTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACAATGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGACTGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCCTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCGTTTATTGGGCAGTTGGTTGAGCCAGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAATTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGA

Protein sequence

MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGLA
Homology
BLAST of Csor.00g266610 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.8e-153
Identity = 275/488 (56.35%), Postives = 352/488 (72.13%), Query Frame = 0

Query: 76  KLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T + V+ + +N S L       L+  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFY 375
           +T+IKA+R+  DGWT F SATV   G+GFIAK ++F N+AGP KHQAVA RS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 376 QCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 435
           +CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 436 QGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPV 495
           QGRE+  + TGISI++ ++ AA DL+PV  +F+ YLGRPW+LYSRTV +KSFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 496 GWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEW 552
           GWL+W   FAL+TLYYGEY+N GPGSN T R+ WPG++ I    EASQF+V  FI G++W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Csor.00g266610 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.2e-145
Identity = 280/547 (51.19%), Postives = 359/547 (65.63%), Query Frame = 0

Query: 21  IFTFFSVIFLCSFKPI---SSNPQ--THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPE 80
           IF   S+IF   F      S +P   TH+T R             ++  + P+L      
Sbjct: 15  IFNLLSLIFFLIFLSTVVSSQSPSYTTHKTQR------------LTETKTIPELII---- 74

Query: 81  KLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYD 140
               + LN T  +V+ A +N S L+T  +  L   +R A +DCL LLD +I++L+T++ D
Sbjct: 75  ----ADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 134

Query: 141 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------TVRKRVELYLGKIEQ 200
           L   +S++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  
Sbjct: 135 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 194

Query: 201 HLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVV 260
           +LS  L ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL V
Sbjct: 195 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 254

Query: 261 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 320
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 255 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 314

Query: 321 KADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSF 380
           KA+R+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS SD SAFY+C F
Sbjct: 315 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 374

Query: 381 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 440
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 375 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 434

Query: 441 DPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLE 500
             +Q TGISILNC++ AA DL+PV  +F+ YLGRPW+ YSRTV +KSFI  L+ P GWLE
Sbjct: 435 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 494

Query: 501 WSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNST 555
               FAL+TLYYGEY+N GPG+N   R+TWPG+R I N TEA+QFTV  FI GS WLNST
Sbjct: 495 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

BLAST of Csor.00g266610 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.9e-143
Identity = 244/319 (76.49%), Postives = 272/319 (85.27%), Query Frame = 0

Query: 237 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 296
           AV +   ++VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V++++KKTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 297 FVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSN 356
           F+GDGIGKT IK +R+VVDGWTTFRS+TVA VG GFIA+GI+FEN+AGPSKHQAVA RS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 357 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 416
           +DFSAFYQCSF+GYQDTLYVHSLRQFY ECDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 417 QRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFI 476
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DL+PVL SF+TYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 477 GQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEA 536
             L++P GWLEWSG FAL TLYY EY N GPGSNTT R+TWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 537 FIQGSEWLNSTEIPFFSGL 556
           FIQGS+WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Csor.00g266610 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 500.0 bits (1286), Expect = 3.5e-140
Identity = 273/529 (51.61%), Postives = 340/529 (64.27%), Query Frame = 0

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRS 341
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I   RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

Query: 342 ATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQF 401
           ATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF
Sbjct: 358 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 417

Query: 402 YRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKV 461
           + +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++
Sbjct: 418 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 477

Query: 462 EAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEY 521
              SDLL V  +F TYLGRPWK YSRTV ++S I  ++ P GW EWSG+FALDTL Y EY
Sbjct: 478 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 537

Query: 522 LNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 552
           LNRG G+ T  R+ W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 538 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Csor.00g266610 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 265/530 (50.00%), Postives = 345/530 (65.09%), Query Frame = 0

Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNNEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L   ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +       R  L  G+  +E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   +++ E   V  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATV 336
           P   T R++I IKAG Y ENVE+ +K  N+MF+GDG  +T+I   RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 359

Query: 337 AAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRE 396
           A VG GF+A+ ITF+N AGPSKHQAVA R  +D SAFY C  + YQDTLYVHS RQF+  
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 397 CDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAA 456
           C + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 479

Query: 457 SDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNR 516
           SDL PV  SF TYLGRPWK YSRTV ++S I  ++ P GW EW G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 539

Query: 517 GPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGL 556
           G G+ T+GR+ W G+RVIT++TEA  FT  +FI GS WL ST  PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Csor.00g266610 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1115 bits (2885), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1108 bits (2865), Expect = 0.0
Identity = 551/556 (99.10%), Postives = 554/556 (99.64%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSV+FLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. NCBI nr
Match: XP_022953706.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1107 bits (2863), Expect = 0.0
Identity = 549/556 (98.74%), Postives = 554/556 (99.64%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSV+FLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV AVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 546/556 (98.20%), Postives = 555/556 (99.82%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSV+ LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKA+RNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGT+DFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 547/556 (98.38%), Postives = 553/556 (99.46%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFT FSV+FL SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRT+FLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 1107 bits (2863), Expect = 0.0
Identity = 549/556 (98.74%), Postives = 554/556 (99.64%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSV+FLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV AVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 547/556 (98.38%), Postives = 553/556 (99.46%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFT FSV+FL SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRT+FLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 556
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Csor.00g266610 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 500/555 (90.09%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQ SSNPT SIS I TFF + FL S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKL+SRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SEWLNSTEIPFFSGL 555
           S WLNST IPFFSGL
Sbjct: 541 SSWLNSTGIPFFSGL 554

BLAST of Csor.00g266610 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 499/555 (89.91%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQ SSNPT SIS I TFF + FL S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVA +G GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKL+SRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SEWLNSTEIPFFSGL 555
           S WLNST IPFFSGL
Sbjct: 541 SSWLNSTGIPFFSGL 554

BLAST of Csor.00g266610 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 938 bits (2424), Expect = 0.0
Identity = 476/561 (84.85%), Postives = 504/561 (89.84%), Query Frame = 0

Query: 6   SRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNP---------QT-HQTARSLCNGTLF 65
           S + SSS+ ++S S+  +  S     S    SS+P         QT HQTARSLCNGTLF
Sbjct: 51  SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPPPPPPLPLFQTFHQTARSLCNGTLF 110

Query: 66  PNLCFSKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRAL 125
           P+LCFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GLNPTDR AL
Sbjct: 111 PDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHAL 170

Query: 126 DDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVR 185
           DDCLELLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR
Sbjct: 171 DDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVR 230

Query: 186 KRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAE-EVLPEYGAVRGGFPMWVSVKDRRLL 245
            RVELYLGKI QHLSIDLAMLKKIPGV RA   + E+LPEYGAV+GGFP WVSVKDRRLL
Sbjct: 231 SRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLL 290

Query: 246 QAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTN 305
           QAAVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+N
Sbjct: 291 QAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSN 350

Query: 306 LMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFR 365
           LMFVGDGIGKTLIKADRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA R
Sbjct: 351 LMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALR 410

Query: 366 SNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPN 425
           SNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYARKPN
Sbjct: 411 SNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPN 470

Query: 426 SNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKS 485
           SNQRNIFTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRTVFL+S
Sbjct: 471 SNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRS 530

Query: 486 FIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTV 545
           FIGQL+EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTT R+TWPGYRVITN+TEASQFTV
Sbjct: 531 FIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTV 590

Query: 546 EAFIQGSEWLNSTEIPFFSGL 555
           E FIQGS WLNSTEIPFFSGL
Sbjct: 591 ERFIQGSSWLNSTEIPFFSGL 611

BLAST of Csor.00g266610 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 543.5 bits (1399), Expect = 2.0e-154
Identity = 275/488 (56.35%), Postives = 352/488 (72.13%), Query Frame = 0

Query: 76  KLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T + V+ + +N S L       L+  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFY 375
           +T+IKA+R+  DGWT F SATV   G+GFIAK ++F N+AGP KHQAVA RS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 376 QCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 435
           +CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 436 QGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPV 495
           QGRE+  + TGISI++ ++ AA DL+PV  +F+ YLGRPW+LYSRTV +KSFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 496 GWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEW 552
           GWL+W   FAL+TLYYGEY+N GPGSN T R+ WPG++ I    EASQF+V  FI G++W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Csor.00g266610 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 515.8 bits (1327), Expect = 4.4e-146
Identity = 280/547 (51.19%), Postives = 359/547 (65.63%), Query Frame = 0

Query: 21  IFTFFSVIFLCSFKPI---SSNPQ--THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPE 80
           IF   S+IF   F      S +P   TH+T R             ++  + P+L      
Sbjct: 15  IFNLLSLIFFLIFLSTVVSSQSPSYTTHKTQR------------LTETKTIPELII---- 74

Query: 81  KLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYD 140
               + LN T  +V+ A +N S L+T  +  L   +R A +DCL LLD +I++L+T++ D
Sbjct: 75  ----ADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSD 134

Query: 141 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------TVRKRVELYLGKIEQ 200
           L   +S++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  
Sbjct: 135 L---RSSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISN 194

Query: 201 HLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVV 260
           +LS  L ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL V
Sbjct: 195 NLSNSLHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSV 254

Query: 261 AKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLI 320
           A DG+GNFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+I
Sbjct: 255 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 314

Query: 321 KADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSF 380
           KA+R+ +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS SD SAFY+C F
Sbjct: 315 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 374

Query: 381 IGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGRE 440
            GYQDTLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R 
Sbjct: 375 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 434

Query: 441 DPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLE 500
             +Q TGISILNC++ AA DL+PV  +F+ YLGRPW+ YSRTV +KSFI  L+ P GWLE
Sbjct: 435 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 494

Query: 501 WSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNST 555
               FAL+TLYYGEY+N GPG+N   R+TWPG+R I N TEA+QFTV  FI GS WLNST
Sbjct: 495 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 531

BLAST of Csor.00g266610 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.5e-141
Identity = 273/529 (51.61%), Postives = 340/529 (64.27%), Query Frame = 0

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRS 341
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I   RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

Query: 342 ATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQF 401
           ATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF
Sbjct: 358 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 417

Query: 402 YRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKV 461
           + +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++
Sbjct: 418 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 477

Query: 462 EAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEY 521
              SDLL V  +F TYLGRPWK YSRTV ++S I  ++ P GW EWSG+FALDTL Y EY
Sbjct: 478 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 537

Query: 522 LNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 552
           LNRG G+ T  R+ W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 538 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Csor.00g266610 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 474.9 bits (1221), Expect = 8.6e-134
Identity = 259/544 (47.61%), Postives = 337/544 (61.95%), Query Frame = 0

Query: 39  NPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSK 98
           +P +H   RS C+ T +P LC S + +   +   S + +  +++N T   V   Y    K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 99  LKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSA 158
           L      GL P ++ AL DCLE +D ++ EL  ++ DL   P++   R H+ DL TL+S+
Sbjct: 112 L-IKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISS 171

Query: 159 AMTNHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPGV----------- 218
           A+TN  TCLDGF +     + R  L  G+I  E   S  LAM+K +              
Sbjct: 172 AITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKI 231

Query: 219 -----------KRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDG 278
                      +    A ++         G+P W+S  DRRLLQ   +  K +  VA DG
Sbjct: 232 TSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADG 291

Query: 279 SGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADR 338
           SG F TV  AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N+MF+GDG  +T+I   R
Sbjct: 292 SGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSR 351

Query: 339 NVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQ 398
           NVVDG TTF SATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFY C  + YQ
Sbjct: 352 NVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQ 411

Query: 399 DTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQ 458
           DTLYVHS RQF+ +C + GT+DFIFGNAAVV Q+C+++AR+PNS Q+N+ TAQGR DPNQ
Sbjct: 412 DTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQ 471

Query: 459 NTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGT 518
           NTGI I  C++ A SDL  V  SF TYLGRPWK YS+TV ++S I  ++ P GW EW+GT
Sbjct: 472 NTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGT 531

Query: 519 FALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 556
           FAL+TL Y EY N G G+ T  R+ W G++VIT + EA ++T   FI G  WL+ST  PF
Sbjct: 532 FALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPF 591

BLAST of Csor.00g266610 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 469.5 bits (1207), Expect = 3.6e-132
Identity = 255/526 (48.48%), Postives = 336/526 (63.88%), Query Frame = 0

Query: 44  QTARSLCNGTLFPNLCFSKLSSFP-QLASLSPEKLAGSALNFT-NNEVHGAYANCSKLKT 103
           Q     C  T FP LC   L  FP  LA+ S + L    +N T ++  H  Y++ S    
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS---- 144

Query: 104 LFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHR 163
           L +  + P  R A D C+ELLD S+  L  +   LS   S++    D+ T +SAA+TNH 
Sbjct: 145 LSFVDMPPRARSAYDSCVELLDDSVDALSRA---LSSVVSSSAKPQDVTTWLSAALTNHD 204

Query: 164 TCLDGFYN-SSGTVRKRVELYLGKIEQHLSIDLAMLKK---------IPGVKRAATAEEV 223
           TC +GF     G V+  +   L  + + +S  LA+            +P   R     E 
Sbjct: 205 TCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEE 264

Query: 224 LPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTT 283
             E       FP W+  K+R +L+  V++ + +++V+KDG+G   T+ EA+  AP +ST 
Sbjct: 265 REE------KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR 324

Query: 284 RFVIYIKAGAYFE-NVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGN 343
           R +IY+KAG Y E N+++ RKK NLMFVGDG GKT+I   +++ D  TTF +A+ AA G 
Sbjct: 325 RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGA 384

Query: 344 GFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYG 403
           GFIA+ ITFEN+AGP+KHQAVA R  +D +  Y+C+ IGYQDTLYVHS RQF+RECD+YG
Sbjct: 385 GFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYG 444

Query: 404 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLP 463
           T+DFIFGNAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL  
Sbjct: 445 TVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQA 504

Query: 464 VLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSN 523
              S +TYLGRPWKL+SRTV++ S+IG  V   GWLEW+ TFALDTLYYGEYLN GPGS 
Sbjct: 505 TNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSG 564

Query: 524 TTGRITWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGLA 557
              R++WPGYRVI ++ EA++FTV  FI GS WL ST + F +GL+
Sbjct: 565 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813012.8e-15356.35Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O814156.2e-14551.19Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
P850769.9e-14376.49Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q425343.5e-14051.61Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839481.3e-13950.00Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6575805.10.0100.00putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp... [more]
KAG7014347.10.099.10putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subs... [more]
XP_022953706.10.098.74pectinesterase-like [Cucurbita moschata][more]
XP_023548978.10.098.20pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022991224.10.098.38pectinesterase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GNU40.098.74Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
A0A6J1JU830.098.38Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A1S3BSU30.090.09Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W60.089.91Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A0A0K5K80.084.85Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02320.12.0e-15456.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.14.4e-14651.19Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.12.5e-14151.61pectin methylesterase 2 [more]
AT3G14310.18.6e-13447.61pectin methylesterase 3 [more]
AT3G49220.13.6e-13248.48Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..195
e-value: 2.7E-29
score: 113.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 20..198
e-value: 2.3E-24
score: 83.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 47..194
e-value: 1.1E-23
score: 84.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 44..201
e-value: 5.3E-28
score: 99.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 47..200
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..540
e-value: 5.3E-145
score: 481.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 3.7E-122
score: 409.7
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 41..552
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 41..552
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..201
e-value: 5.23487E-37
score: 132.562
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 242..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g266610.m01Csor.00g266610.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity