Csor.00g264380 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g264380
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionO-methyltransferase
LocationCsor_Chr11: 13203860 .. 13205072 (-)
RNA-Seq ExpressionCsor.00g264380
SyntenyCsor.00g264380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACGTAGCGAGAAAGAAACGTTGTCGAAGAAGGAAGAAGAAGATGGGCAAGCCATCATCCAAATGTGGAAATACATATTTGGTTTCTCTGAAATGGCAGCCATAAAATGTGCCATAGAGCTTCAAATAGCTGATATCATTGAAGACCATGGCAGCCCCATGACATTGCCCCAACTCTCCTCCACTCTAAACTGTTCTTTACCTCTCCTGCATCGAATCTTAAGATTCCTCGTCCGTCGAGGAATCTTCAAGGAATATTCCATATCCGACGACCTGATCGGCTATGCTCAGACGCCTCTATCGCGCTTGCTCGCCACCAAGAACGGCAGCAGCTTGGCCCCCTTCATGCTGCTGGAAGCCAGCCCGGCGATGCTTGCAGCATGGCATAACTTGAGCGCGCACGTCAAGAGTGGCGGCAGGTTCCCATTTGAGGCTGCGCATGGCATGGACGTGTGGGGCTATGCTGCGGCTAATCCGAAGCATAATAGGCTGATTAATGAGGCCATGGGGTGCTCTACGGGGCTGGTTGTGGCTGCTATACTTGATGGTTGTCAAGGGATTTTTGATGGGGTTGGGAGTTTGGTTGATGTGGGTGGAGGAAATGGTGCTACTTTGAGTGTTGTTGTTAAGGCGTGTTCGTGGATTCGAGGGATTAATTTCGATCTTTCTCATGTTGTTTCTACGTCGCCTGTGTATGAACGTGTGGAGCATGTTGCTGGTGATATGTTTGATTTTGTTCCGAATGCTGATGCTGCTTTCATTAAGGTGTGTATCTATGTTTTTTTGTTGTCGGGTCGTAGATGTTGCATAATTGTGTTGTGAAGCTCAATAGAGTGTGATTGTGACGGTTTTTTAATTTTCGAGTTTAAGGTAGAGATTGTTTAAATTATACTTGAAATATGTTTGATGGCAGTGGACTCTACATGATTGGAACGACGAAGAATGTATTACTATTTTGAAGAATTGTAAGGAGGCGATTCCGAAGAACACAGGTAAAGTGATCATTGTTGATGCAGTAATCGATGAAAAAGAAGAAACCGAGCAATCAGATGTTGGATTGATGTTGGACTTGGTGATGATGGCTCATACTAGCACAGGCAAAGAAAGAACACGTAAAGAATGGGAATATGTTATTAATGCAGCTGGTTTCAGTCGATTCACTGTTACACGCATTCAAGCTATTCAATCAGTTATCGAGTGCTTCCCATAA

mRNA sequence

ATGGAACGTAGCGAGAAAGAAACGTTGTCGAAGAAGGAAGAAGAAGATGGGCAAGCCATCATCCAAATGTGGAAATACATATTTGGTTTCTCTGAAATGGCAGCCATAAAATGTGCCATAGAGCTTCAAATAGCTGATATCATTGAAGACCATGGCAGCCCCATGACATTGCCCCAACTCTCCTCCACTCTAAACTGTTCTTTACCTCTCCTGCATCGAATCTTAAGATTCCTCGTCCGTCGAGGAATCTTCAAGGAATATTCCATATCCGACGACCTGATCGGCTATGCTCAGACGCCTCTATCGCGCTTGCTCGCCACCAAGAACGGCAGCAGCTTGGCCCCCTTCATGCTGCTGGAAGCCAGCCCGGCGATGCTTGCAGCATGGCATAACTTGAGCGCGCACGTCAAGAGTGGCGGCAGGTTCCCATTTGAGGCTGCGCATGGCATGGACGTGTGGGGCTATGCTGCGGCTAATCCGAAGCATAATAGGCTGATTAATGAGGCCATGGGGTGCTCTACGGGGCTGGTTGTGGCTGCTATACTTGATGGTTGTCAAGGGATTTTTGATGGGGTTGGGAGTTTGGTTGATGTGGGTGGAGGAAATGGTGCTACTTTGAGTGTTGTTGTTAAGGCGTGTTCGTGGATTCGAGGGATTAATTTCGATCTTTCTCATGTTGTTTCTACGTCGCCTGTGTATGAACGTGTGGAGCATGTTGCTGGTGATATGTTTGATTTTGTTCCGAATGCTGATGCTGCTTTCATTAAGTGGACTCTACATGATTGGAACGACGAAGAATGTATTACTATTTTGAAGAATTGTAAGGAGGCGATTCCGAAGAACACAGGTAAAGTGATCATTGTTGATGCAGTAATCGATGAAAAAGAAGAAACCGAGCAATCAGATGTTGGATTGATGTTGGACTTGGTGATGATGGCTCATACTAGCACAGGCAAAGAAAGAACACGTAAAGAATGGGAATATGTTATTAATGCAGCTGGTTTCAGTCGATTCACTGTTACACGCATTCAAGCTATTCAATCAGTTATCGAGTGCTTCCCATAA

Coding sequence (CDS)

ATGGAACGTAGCGAGAAAGAAACGTTGTCGAAGAAGGAAGAAGAAGATGGGCAAGCCATCATCCAAATGTGGAAATACATATTTGGTTTCTCTGAAATGGCAGCCATAAAATGTGCCATAGAGCTTCAAATAGCTGATATCATTGAAGACCATGGCAGCCCCATGACATTGCCCCAACTCTCCTCCACTCTAAACTGTTCTTTACCTCTCCTGCATCGAATCTTAAGATTCCTCGTCCGTCGAGGAATCTTCAAGGAATATTCCATATCCGACGACCTGATCGGCTATGCTCAGACGCCTCTATCGCGCTTGCTCGCCACCAAGAACGGCAGCAGCTTGGCCCCCTTCATGCTGCTGGAAGCCAGCCCGGCGATGCTTGCAGCATGGCATAACTTGAGCGCGCACGTCAAGAGTGGCGGCAGGTTCCCATTTGAGGCTGCGCATGGCATGGACGTGTGGGGCTATGCTGCGGCTAATCCGAAGCATAATAGGCTGATTAATGAGGCCATGGGGTGCTCTACGGGGCTGGTTGTGGCTGCTATACTTGATGGTTGTCAAGGGATTTTTGATGGGGTTGGGAGTTTGGTTGATGTGGGTGGAGGAAATGGTGCTACTTTGAGTGTTGTTGTTAAGGCGTGTTCGTGGATTCGAGGGATTAATTTCGATCTTTCTCATGTTGTTTCTACGTCGCCTGTGTATGAACGTGTGGAGCATGTTGCTGGTGATATGTTTGATTTTGTTCCGAATGCTGATGCTGCTTTCATTAAGTGGACTCTACATGATTGGAACGACGAAGAATGTATTACTATTTTGAAGAATTGTAAGGAGGCGATTCCGAAGAACACAGGTAAAGTGATCATTGTTGATGCAGTAATCGATGAAAAAGAAGAAACCGAGCAATCAGATGTTGGATTGATGTTGGACTTGGTGATGATGGCTCATACTAGCACAGGCAAAGAAAGAACACGTAAAGAATGGGAATATGTTATTAATGCAGCTGGTTTCAGTCGATTCACTGTTACACGCATTCAAGCTATTCAATCAGTTATCGAGTGCTTCCCATAA

Protein sequence

MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLEASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP
Homology
BLAST of Csor.00g264380 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.8e-97
Identity = 186/368 (50.54%), Postives = 250/368 (67.93%), Query Frame = 0

Query: 1   MERSEKETLSKKE-EEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGS--PMTL 60
           ++R++ E   +K  +E+ +A + +WKY+FGF+++AA KCAI+L+I + IE+H S  P+TL
Sbjct: 10  LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69

Query: 61  PQLSSTLNCSLPLLHRILRFLVRRGIFKEYSISDDL-IGYAQTPLSR--LLATKNGSSLA 120
            +LSS ++ S   L RI+RFLV +GIFKE    D L  GY  TPLSR  ++  ++G SLA
Sbjct: 70  AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129

Query: 121 PFMLLEASPAMLAAWHNLSAHVKS----GGRFPFEAAHGMDVWGYAAANPKHNRLINEAM 180
           PF+L E +P MLA W  LS+ V S        PF+A HG DVW +A  NP  + +INEAM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189

Query: 181 GCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTS 240
            C    VV  +   C G+FDGV ++VDVGGG G T+ ++VK   WI+G NFDL HV+  +
Sbjct: 190 ACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 249

Query: 241 PVYERVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDA 300
            V + VE+V GDMFD +P  DA FIKW LHDW D++CI ILKNCKEA+P N GKV+IV++
Sbjct: 250 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 309

Query: 301 VIDEKEETEQSD--------VGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTR 351
           VI E ++T   D        V LMLD+VMMAHTSTGKERT KEW++V+  AGF+R+ V  
Sbjct: 310 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 369

BLAST of Csor.00g264380 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.5e-72
Identity = 156/358 (43.58%), Postives = 214/358 (59.78%), Query Frame = 0

Query: 12  KEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLNCSLP-- 71
           +E   GQA  ++W+++F F +  A+KCA+EL I DII  HG P+T+ ++  +L  + P  
Sbjct: 5   EERLKGQA--EIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSS 64

Query: 72  -----LLHRILRFLVRRGIF-KEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLEASP 131
                 L RI+R LV + +F  E     + + Y  T  S+ L   +  +L+P +L E +P
Sbjct: 65  SPNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNP 124

Query: 132 AMLAAWHNLS--AHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAAI 191
            +L  W  L   A  KS    PFE AHG ++W  A A+PK N  +N AM CST  ++  +
Sbjct: 125 ILLKPWQYLGKCAQEKSS---PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEM 184

Query: 192 L----DGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVE 251
           L    DG  GI    GSLVDVGGG G+ ++ +VKA   I+GINFDL HVV+T+  +  V+
Sbjct: 185 LLEYKDGFSGI---AGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVK 244

Query: 252 HVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAI-PKNTGKVIIVDAVIDEKE 311
           HV GDMF  +P ADA  +KW LHDW+DE+C  ILKNC  AI  K  GKVIIVD V+    
Sbjct: 245 HVGGDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDG 304

Query: 312 ETEQSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
                 +GL+ D++MMAHT+ GKERT  EW+ ++N AGF R+ V R  A   +IE FP
Sbjct: 305 NDLFDKMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of Csor.00g264380 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.4e-72
Identity = 146/343 (42.57%), Postives = 216/343 (62.97%), Query Frame = 0

Query: 23  MWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTL----NCSLPLLHRILRFL 82
           +WK I G ++   +KCA+EL+I DI+  H +P+TL Q++S++    + +L  L RI+R L
Sbjct: 19  VWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSRIMRLL 78

Query: 83  VRRGIFKEY-SISDDLIGYAQTPLSRLLATK----NGSSLAPFMLLEASPAMLAAWHNLS 142
           VRR IF ++ S+  + + Y  T  SRLL +K    +  +LAPF+     P + A W  L+
Sbjct: 79  VRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLA 138

Query: 143 AHVKSGGRFPFEAAH-GMDVWGYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGV 202
             VK GG   FE  H G  +W  +  NP+ N++ N+ M  +  +   AIL   + +F G+
Sbjct: 139 RCVKEGGN-GFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTMAILSEYRDVFCGI 198

Query: 203 GSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYE-RVEHVAGDMFDFVPNAD 262
            SLVDVGG  G ++S +VK+   I+GIN+DL HVV+T+P Y   V HV G+MF+++P A 
Sbjct: 199 CSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNMFEWIPTAV 258

Query: 263 AAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLMLDLVM 322
           A F+KW LHDW DE+C+ ILKNC+ A+P+  GK+IIVD V++ +      D  +MLD+ +
Sbjct: 259 AVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLEPEGNGLFDDAAVMLDIAL 318

Query: 323 MAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
           MA T  GKERT KEW+ V+   GF R+ + +I A+ SVIE +P
Sbjct: 319 MALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

BLAST of Csor.00g264380 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 144/360 (40.00%), Postives = 218/360 (60.56%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME   KE LS +         ++W +I+GF++   +K A++L +A+II +HGSPMTL +L
Sbjct: 1   MEMINKENLSSQ--------AKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSEL 60

Query: 61  SSTLNCSLPL----LHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPF 120
           S  L  S P+    L+R+LR+LV   +F + SI  +L  Y   P ++ L       +   
Sbjct: 61  SLHLP-SQPVNQDALYRVLRYLVHMKLFTKSSIDGEL-RYGLAPPAKFLVKGWDKCMLGA 120

Query: 121 MLLEASPAMLAAWHNLSAHVKSGG--RFPFEAAHGMDVWGYAAANPKHNRLINEAMGCST 180
           +L       +A WH L   + + G     FE A G ++W Y A +P+ N+L NE M   T
Sbjct: 121 ILTITDKDFMAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDT 180

Query: 181 GLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYE 240
            L+++A++  C  +FDG+ ++VDVGGG G  +  + KA   I+   +DL HV++ SP Y 
Sbjct: 181 RLIMSALVKECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYT 240

Query: 241 RVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDE 300
            +  + GDMF ++PNADA  +K  LHDW+D+ECI ILK CK+A+P++ GKVII+D ++D 
Sbjct: 241 EINSIQGDMFKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDV 300

Query: 301 KEETEQSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
           K E   + + L LDL MM +T  GKERT +EW+ +I+ AG+  + +T I A+QSVIE +P
Sbjct: 301 KSEHPYTKMRLTLDLDMMLNTG-GKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349

BLAST of Csor.00g264380 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.3e-70
Identity = 145/348 (41.67%), Postives = 213/348 (61.21%), Query Frame = 0

Query: 11  KKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLNCSLPL 70
           KK+    QA  ++W +I+GF+E   +KCA++L +A+II + G+ MTL +LSS L  S P+
Sbjct: 4   KKDNLSSQA--KLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLP-SQPV 63

Query: 71  ----LHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLEASPAML 130
               L+R++R+LV   +F + SI  +L  Y   P ++ L       +   +L       +
Sbjct: 64  NEDALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKYLVKGWDKCMVGSILAITDKDFM 123

Query: 131 AAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAAILDGCQ 190
           A WH L   +       FE A G ++WGY A +P+ N+L NEAM   + L+++A++  C 
Sbjct: 124 APWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECG 183

Query: 191 GIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVAGDMFDF 250
            IF+G+ +LVDVGGG G  +  +  A   I+   +DL HV++ SP Y  V  VAGDMF F
Sbjct: 184 NIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKF 243

Query: 251 VPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLM 310
           +P ADA  +K  LHDW+D+ECI ILK CKEA+P   GKVIIVD V++ + E   + + L 
Sbjct: 244 IPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLT 303

Query: 311 LDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
           LDL MM +T  GKERT +EW+ +I+ AG+    +T+I A+QSVIE +P
Sbjct: 304 LDLDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

BLAST of Csor.00g264380 vs. NCBI nr
Match: KAG6589030.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 708 bits (1828), Expect = 2.12e-257
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL
Sbjct: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE
Sbjct: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP
Sbjct: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Csor.00g264380 vs. NCBI nr
Match: XP_022928138.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucurbita moschata] >XP_022928139.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 696 bits (1796), Expect = 1.60e-252
Identity = 348/354 (98.31%), Postives = 350/354 (98.87%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME +EKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL
Sbjct: 1   MEGTEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTL+CS PLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE
Sbjct: 61  SSTLHCSSPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDL HVVSTSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVVSTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHTSTGKERTRKEW YVINAAGFSRFTVTRIQAIQSVIECFP
Sbjct: 301 SDVGLMLDLVMMAHTSTGKERTRKEWAYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Csor.00g264380 vs. NCBI nr
Match: XP_023529744.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692 bits (1785), Expect = 7.60e-251
Identity = 346/354 (97.74%), Postives = 349/354 (98.59%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           MERSE+E+LSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHG PMTLPQL
Sbjct: 1   MERSERESLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGGPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTL+CS PLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE
Sbjct: 61  SSTLHCSSPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHN LINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNGLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHTS GKERTRKEWEYVINAAGFSRFTVT IQAIQSVIECFP
Sbjct: 301 SDVGLMLDLVMMAHTSRGKERTRKEWEYVINAAGFSRFTVTCIQAIQSVIECFP 354

BLAST of Csor.00g264380 vs. NCBI nr
Match: XP_022989430.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 672 bits (1734), Expect = 4.34e-243
Identity = 338/354 (95.48%), Postives = 344/354 (97.18%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           MER++KETLSKKEEE GQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL
Sbjct: 1   MERTDKETLSKKEEE-GQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTL+CS PLLHRILRFLV RGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAP MLLE
Sbjct: 61  SSTLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSA+VKSGGRFPFEAAHGMDVW YAAANPKHNRLINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDL HV+STSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHT+TGKERTRKEW YVINA GFSRFTVT IQAIQSVIEC P
Sbjct: 301 SDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 353

BLAST of Csor.00g264380 vs. NCBI nr
Match: XP_038887538.1 (acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 496 bits (1278), Expect = 1.25e-173
Identity = 240/346 (69.36%), Postives = 283/346 (81.79%), Query Frame = 0

Query: 10  SKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQLSSTLNCSLP 69
           S K EEDGQAI+QMW+YIF F+EMAAIKC I+L+IADIIE +GSPMTL  LSS LNCS  
Sbjct: 9   SSKTEEDGQAIVQMWRYIFRFTEMAAIKCVIDLKIADIIESYGSPMTLSHLSSALNCSSS 68

Query: 70  LLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLEASPAMLAAW 129
           LLHRILRFL+ RGIFKE +I ++   Y+QTPLSRLLAT   S++AP +LLE SP MLAAW
Sbjct: 69  LLHRILRFLIHRGIFKEETIGENQTAYSQTPLSRLLATNVESTMAPLLLLETSPVMLAAW 128

Query: 130 HNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIF 189
            NLSAH+K+   FPFE AHG D+W YA ANP+HN L NEAM C   ++  AIL+GC  IF
Sbjct: 129 PNLSAHLKNSETFPFEIAHGKDLWSYAEANPEHNVLFNEAMACYAKVIAFAILEGCGDIF 188

Query: 190 DGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVAGDMFDFVPN 249
           DGVG LVDVGGGNG+TLS++V AC W++GINFDL HVV  SP YE V+HVAG+MFDFVP 
Sbjct: 189 DGVGCLVDVGGGNGSTLSILVNACPWMKGINFDLPHVVCASPQYENVQHVAGNMFDFVPK 248

Query: 250 ADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ-SDVGLMLD 309
           AD AF+KW LHDWNDEECI ILK CKEAIPK+ GKVIIV+A+I+EK E ++ SDVGLM D
Sbjct: 249 ADVAFLKWILHDWNDEECIKILKRCKEAIPKSGGKVIIVEAIIEEKSENKKVSDVGLMFD 308

Query: 310 LVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           LVMMAHT+ GKERT +EW +VINAAGF+R+T+T IQ IQS+I+CFP
Sbjct: 309 LVMMAHTNKGKERTIEEWAFVINAAGFTRYTITPIQPIQSLIQCFP 354

BLAST of Csor.00g264380 vs. ExPASy TrEMBL
Match: A0A6J1EN16 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111435052 PE=3 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 7.74e-253
Identity = 348/354 (98.31%), Postives = 350/354 (98.87%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME +EKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL
Sbjct: 1   MEGTEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTL+CS PLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE
Sbjct: 61  SSTLHCSSPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDL HVVSTSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVVSTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHTSTGKERTRKEW YVINAAGFSRFTVTRIQAIQSVIECFP
Sbjct: 301 SDVGLMLDLVMMAHTSTGKERTRKEWAYVINAAGFSRFTVTRIQAIQSVIECFP 354

BLAST of Csor.00g264380 vs. ExPASy TrEMBL
Match: A0A6J1JMC1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111486486 PE=3 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 2.10e-243
Identity = 338/354 (95.48%), Postives = 344/354 (97.18%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           MER++KETLSKKEEE GQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL
Sbjct: 1   MERTDKETLSKKEEE-GQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTL+CS PLLHRILRFLV RGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAP MLLE
Sbjct: 61  SSTLHCSSPLLHRILRFLVSRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPLMLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
           ASPAMLAAWHNLSA+VKSGGRFPFEAAHGMDVW YAAANPKHNRLINEAMGCSTGLVVAA
Sbjct: 121 ASPAMLAAWHNLSAYVKSGGRFPFEAAHGMDVWRYAAANPKHNRLINEAMGCSTGLVVAA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDL HV+STSPVYERVEHVA
Sbjct: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLPHVISTSPVYERVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ
Sbjct: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300

Query: 301 SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
           SDVGLMLDLVMMAHT+TGKERTRKEW YVINA GFSRFTVT IQAIQSVIEC P
Sbjct: 301 SDVGLMLDLVMMAHTNTGKERTRKEWAYVINAVGFSRFTVTCIQAIQSVIECIP 353

BLAST of Csor.00g264380 vs. ExPASy TrEMBL
Match: A0A5A7TGF8 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004180 PE=3 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 1.47e-168
Identity = 236/359 (65.74%), Postives = 291/359 (81.06%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME   KE+ S+KEE DGQAIIQMW+YIF F+EMAAIKCAI+L+IADIIE +GSP+TL QL
Sbjct: 1   MEVKHKESSSEKEE-DGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTLNCS  LL+RILRFL+ RGIFK  +I ++ IGY+QTP+SRLLAT   +S+AP +LLE
Sbjct: 61  SSTLNCSSSLLYRILRFLIHRGIFKRETIDENQIGYSQTPMSRLLATNVENSMAPLLLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
            SP MLA W +LSAH+K+    PFE AHG D+W YA AN +HN L NEAM CS  ++V+A
Sbjct: 121 TSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           I++GC  +FDGVG LVDVGGGNG+TL+++VK C W++GINFDL HVV  SP YE VEHVA
Sbjct: 181 IIEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           G+MFDFVP+AD AF+KW LHDW DEECI ILK CKEAIPK+ GKVII++A+I E E+ E+
Sbjct: 241 GNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEK 300

Query: 301 -----SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
                SDVGLM DLVMMAHT+ G+ERT +EW ++I+ AGF+R T+T +QAIQS+I+CFP
Sbjct: 301 MKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of Csor.00g264380 vs. ExPASy TrEMBL
Match: A0A1S3BJE6 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490250 PE=3 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 1.20e-167
Identity = 235/359 (65.46%), Postives = 290/359 (80.78%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME   KE+ S+KEE DGQAIIQMW+YIF F+EMAAIKCAI+L+IADIIE +GSP+TL QL
Sbjct: 1   MEVKHKESSSEKEE-DGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTLNCS  LL+RILRFL+ RGIFK  +I ++ IGY+QTP+SRLLA    +S+AP +LLE
Sbjct: 61  SSTLNCSSSLLYRILRFLIHRGIFKRETIDENQIGYSQTPMSRLLAINVENSMAPLLLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
            SP MLA W +LSAH+K+    PFE AHG D+W YA AN +HN L NEAM CS  ++V+A
Sbjct: 121 TSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVSA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           I++GC  +FDGVG LVDVGGGNG+TL+++VK C W++GINFDL HVV  SP YE VEHVA
Sbjct: 181 IIEGCGDVFDGVGCLVDVGGGNGSTLNILVKVCPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQ 300
           G+MFDFVP+AD AF+KW LHDW DEECI ILK CKEAIPK+ GKVII++A+I E E+ E+
Sbjct: 241 GNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAEKGEK 300

Query: 301 -----SDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
                SDVGLM DLVMMAHT+ G+ERT +EW ++I+ AGF+R T+T +QAIQS+I+CFP
Sbjct: 301 MKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQCFP 358

BLAST of Csor.00g264380 vs. ExPASy TrEMBL
Match: A0A0A0K629 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 478 bits (1230), Expect = 1.35e-166
Identity = 233/358 (65.08%), Postives = 288/358 (80.45%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGSPMTLPQL 60
           ME   KE+ S+KEE DGQAIIQMW+YIF F+EMAAIKC ++L+IADIIE +GSP+TL QL
Sbjct: 1   MEVKHKESSSEKEE-DGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQL 60

Query: 61  SSTLNCSLPLLHRILRFLVRRGIFKEYSISDDLIGYAQTPLSRLLATKNGSSLAPFMLLE 120
           SSTLNCS  LLHRILRFL+ RGIFK+ +I ++ IGY+QTP+SRLLAT   +S+AP +LLE
Sbjct: 61  SSTLNCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLE 120

Query: 121 ASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAA 180
            SP MLA W +LS H+K+    PFE AHG D+W YA AN +HN L NEAM C   ++V+A
Sbjct: 121 TSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVSA 180

Query: 181 ILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVA 240
           I++GC  +FDGV  LVDVGGGNG+TLS++VKA  W++GINFDL HVV  SP YE VEHVA
Sbjct: 181 IIEGCGDVFDGVRCLVDVGGGNGSTLSILVKAYPWMKGINFDLPHVVCASPQYENVEHVA 240

Query: 241 GDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVID----EKE 300
           G+MFDFVPNAD AF+KW LHDW+DEECI ILK CKE+IPK+ GKVII++A+I+    EK+
Sbjct: 241 GNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEKGEKK 300

Query: 301 ETEQSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 354
             + SDVGLM DLVMMAHT+ GKERT +EW ++I+ AGF+  T+T IQAIQS+I+CFP
Sbjct: 301 NKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQCFP 357

BLAST of Csor.00g264380 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 355.5 bits (911), Expect = 4.9e-98
Identity = 186/368 (50.54%), Postives = 250/368 (67.93%), Query Frame = 0

Query: 1   MERSEKETLSKKE-EEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGS--PMTL 60
           ++R++ E   +K  +E+ +A + +WKY+FGF+++AA KCAI+L+I + IE+H S  P+TL
Sbjct: 10  LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69

Query: 61  PQLSSTLNCSLPLLHRILRFLVRRGIFKEYSISDDL-IGYAQTPLSR--LLATKNGSSLA 120
            +LSS ++ S   L RI+RFLV +GIFKE    D L  GY  TPLSR  ++  ++G SLA
Sbjct: 70  AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129

Query: 121 PFMLLEASPAMLAAWHNLSAHVKS----GGRFPFEAAHGMDVWGYAAANPKHNRLINEAM 180
           PF+L E +P MLA W  LS+ V S        PF+A HG DVW +A  NP  + +INEAM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189

Query: 181 GCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTS 240
            C    VV  +   C G+FDGV ++VDVGGG G T+ ++VK   WI+G NFDL HV+  +
Sbjct: 190 ACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 249

Query: 241 PVYERVEHVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDA 300
            V + VE+V GDMFD +P  DA FIKW LHDW D++CI ILKNCKEA+P N GKV+IV++
Sbjct: 250 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 309

Query: 301 VIDEKEETEQSD--------VGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTR 351
           VI E ++T   D        V LMLD+VMMAHTSTGKERT KEW++V+  AGF+R+ V  
Sbjct: 310 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 369

BLAST of Csor.00g264380 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.4e-83
Identity = 164/361 (45.43%), Postives = 220/361 (60.94%), Query Frame = 0

Query: 1   MERSEKETLSKKEEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADIIEDHGS--PMTLP 60
           ME S++  L    +E+ +A + +W+Y+FGF+++AA KCAI+L+I + IE+H S  P+TL 
Sbjct: 1   MEESKRNLL----DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLS 60

Query: 61  QLSSTLNCSLPLLHRILRFLVRRGIFKEYSISDDL-IGYAQTPLSRLLATKNGSSLAPFM 120
           +LSS ++ S   L RI+RFLV +G+FKE    D L  GY  TPLSR              
Sbjct: 61  ELSSAVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSR-------------- 120

Query: 121 LLEASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLV 180
                                  R      HG D+W +A  N  H++LINEAM C    V
Sbjct: 121 -----------------------RMMITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV 180

Query: 181 VAAILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVE 240
           V  +   CQG+FDGV ++VDVGGG G T+ ++VK   WI+G NFDL HV+  + V + VE
Sbjct: 181 VPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVE 240

Query: 241 HVAGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVIDEKEE 300
           +V GDMFD +P +DA  IKW LHDW D++CI ILKNCKEA+  N GKV+IV+ VI EK+ 
Sbjct: 241 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 300

Query: 301 T----EQSD----VGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSV 351
           T    E+ D    V L LD+VMM HTSTGKERT KEW++V+  AGF+R+ V     +QS+
Sbjct: 301 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSL 320

BLAST of Csor.00g264380 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 210.7 bits (535), Expect = 1.9e-54
Identity = 120/317 (37.85%), Postives = 186/317 (58.68%), Query Frame = 0

Query: 35  AIKCAIELQIADIIEDHGSPMTLPQLSSTLNCSLP----LLHRILRFLVRRGIF--KEYS 94
           A+K A+EL + +I+  +GSPM+  +++S L    P    +L RILR L    +       
Sbjct: 35  ALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRK 94

Query: 95  ISDDLIG--YAQTPLSRLLATKN--GSSLAPFMLLEASPAMLAAWHNLSAHVKSGGRFPF 154
           +S D +   Y   P+ + L TKN  G S+A   L+     ++ +W++L   +  GG  PF
Sbjct: 95  LSGDGVERIYGLGPVCKYL-TKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG-IPF 154

Query: 155 EAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVAAILDGCQGIFDGVGSLVDVGGGNGA 214
             A+GM  + Y   +P+ N++ N  M   + + +  IL+  +G F+G+ SLVDVGGG GA
Sbjct: 155 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 214

Query: 215 TLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHVAGDMFDFVPNADAAFIKWTLHDWND 274
           TL ++V     ++GINFDL HV+  +P +  +EHV GDMF  VP  DA F+KW  HDW+D
Sbjct: 215 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSD 274

Query: 275 EECITILKNCKEAIPKNTGKVIIVDAVIDEKEETEQSDVGLM-LDLVMMAHTSTGKERTR 334
           E C+  LKNC E++P++ GKVI+ + ++ E  ++  S   ++ +D +M+AH   GKERT 
Sbjct: 275 EHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTE 334

Query: 335 KEWEYVINAAGFSRFTV 341
           KE+E +  A+GF    V
Sbjct: 335 KEFEALAKASGFKGIKV 347

BLAST of Csor.00g264380 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 193.0 bits (489), Expect = 4.2e-49
Identity = 122/357 (34.17%), Postives = 194/357 (54.34%), Query Frame = 0

Query: 13  EEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADI------IEDHGSPMTLPQLSSTLNC 72
           EEED    IQ+    F       +K A EL + +I      +  + SP+ L  +++  N 
Sbjct: 12  EEEDMLLAIQLGGLNF---VPYIVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNP 71

Query: 73  SLP-LLHRILRFLVRRGIFKEYSISDD------LIGYAQTPLSRLLATKNGSSLAPFMLL 132
             P ++ R+LRFLV   +     + D+        G  +    +L+  ++G S+AP++L 
Sbjct: 72  HAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG-KKLIKDEDGFSIAPYVLA 131

Query: 133 EASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVA 192
             + A    W+N+   ++ GG   +E A+   ++ Y   N    ++ NE+M   T +V+ 
Sbjct: 132 GCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMK 191

Query: 193 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHV 252
            IL+   G F+GV   VDVGG  G+ L+ ++     I+GINFDL H+V  +P    VEH+
Sbjct: 192 KILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHI 251

Query: 253 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVID-EKEET 312
            GDMFD +P  +   +KW LHDWNDE+C+ ILKNCK+A+P+ TG++I+++ ++  E  ET
Sbjct: 252 GGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSET 311

Query: 313 E-QSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
           +  +   L  DL MM+ TS GKERT+KE+E +   AGF    +        +IE +P
Sbjct: 312 DLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of Csor.00g264380 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.1e-48
Identity = 122/357 (34.17%), Postives = 193/357 (54.06%), Query Frame = 0

Query: 13  EEEDGQAIIQMWKYIFGFSEMAAIKCAIELQIADI------IEDHGSPMTLPQLSSTLNC 72
           EEED    IQ+    F       +K A EL + +I      +  + SP+ L  +++  N 
Sbjct: 12  EEEDMLLAIQLGGLNF---VPYIVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNP 71

Query: 73  SLP-LLHRILRFLVRRGIFKEYSISDD------LIGYAQTPLSRLLATKNGSSLAPFMLL 132
             P ++ R+LRFLV   +     + D+        G  +    +L+  ++G S+AP++L 
Sbjct: 72  HAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG-KKLIKDEDGFSIAPYVLA 131

Query: 133 EASPAMLAAWHNLSAHVKSGGRFPFEAAHGMDVWGYAAANPKHNRLINEAMGCSTGLVVA 192
             + A    W  L+  ++ GG   +E A+   ++ Y   N    ++ NE+M   T +V+ 
Sbjct: 132 GCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMK 191

Query: 193 AILDGCQGIFDGVGSLVDVGGGNGATLSVVVKACSWIRGINFDLSHVVSTSPVYERVEHV 252
            IL+   G F+GV   VDVGG  G+ L+ ++     I+GINFDL H+V  +P    VEH+
Sbjct: 192 KILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHI 251

Query: 253 AGDMFDFVPNADAAFIKWTLHDWNDEECITILKNCKEAIPKNTGKVIIVDAVID-EKEET 312
            GDMFD +P  +   +KW LHDWNDE+C+ ILKNCK+A+P+ TG++I+++ ++  E  ET
Sbjct: 252 GGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSET 311

Query: 313 E-QSDVGLMLDLVMMAHTSTGKERTRKEWEYVINAAGFSRFTVTRIQAIQSVIECFP 355
           +  +   L  DL MM+ TS GKERT+KE+E +   AGF    +        +IE +P
Sbjct: 312 DLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0036.8e-9750.54Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... [more]
Q6WUC21.5e-7243.58(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... [more]
B0ZB563.4e-7242.57Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1[more]
Q5C9L71.1e-7040.00(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=... [more]
Q9LEL69.3e-7041.67(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6589030.12.12e-257100.00Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022928138.11.60e-25298.31(R,S)-reticuline 7-O-methyltransferase-like [Cucurbita moschata] >XP_022928139.1... [more]
XP_023529744.17.60e-25197.74(R,S)-reticuline 7-O-methyltransferase-like [Cucurbita pepo subsp. pepo][more]
XP_022989430.14.34e-24395.48(RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita maxima][more]
XP_038887538.11.25e-17369.36acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EN167.74e-25398.31(R,S)-reticuline 7-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JMC12.10e-24395.48(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7TGF81.47e-16865.74(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BJE61.20e-16765.46(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0K6291.35e-16665.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018730 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35160.14.9e-9850.54O-methyltransferase family protein [more]
AT4G35150.14.4e-8345.43O-methyltransferase family protein [more]
AT5G54160.11.9e-5437.85O-methyltransferase 1 [more]
AT1G51990.24.2e-4934.17O-methyltransferase family protein [more]
AT1G51990.12.1e-4834.17O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 1..354
e-value: 1.6E-123
score: 410.1
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 8..348
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 24..354
score: 77.176323
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 5..105
e-value: 1.8E-26
score: 93.7
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 35..79
e-value: 6.7E-11
score: 42.0
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 129..336
e-value: 5.4E-54
score: 182.7
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 107..354
e-value: 1.1E-78
score: 265.8
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 34..352
e-value: 8.3E-91
score: 301.9
NoneNo IPR availablePANTHERPTHR11746:SF1913'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKEcoord: 8..348
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 13..109
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 110..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g264380.m01Csor.00g264380.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity