Csor.00g264080 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g264080
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAmidase domain-containing protein
LocationCsor_Chr11: 13003057 .. 13006986 (+)
RNA-Seq ExpressionCsor.00g264080
SyntenyCsor.00g264080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAACTTTCCAGGATCCAATCCTTTTCCTCTACAAATCCAGACAGTCCCAATCTCCGTCCATACGCTCCTACCGCCGCCGCCGTCGCCGCCACCATCCAAAAATGAACATCATCTTCTTCCTCTCAGTTTTCTTACTCTTCGTCGGAGCCAACAGTTCTCAATTTCCGATCGACGAAGCAACCATCGCCGAAATCCAACACGCTTTCTCCCAAAACAAGCTCACCTCCAGAGAACTTCTCGATCACTACCTGAACAAGATCGATTTTCTTAATCCAGTGCTTAGAAGCGTTCTCGAAGTGAATCCTGATGCGAGAGCCCAAGCGGAAGCCGCCGATCGAGAGAGGGAGCTCGCTGGAGGAAAAGGCCTTGGCGAACTCCATGGAATTCCAGTGCTGCTCAAGGATTCAATTGGAACCAAGGATCGGCTCAATACCACGGCCGGTTCCTTCGCATTGTTAGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTCCACCGCCTAAGGAACGCCGGTGCGGTGATTTTGGGGAAAACTTCGCTCACTGAGTGGTATGGGTCTCGGTCTCTGCAGATTCCACATGGCTGGTGCGCTCGCGGCGGTCAAGCCCTGGTTAGTAATTGAATGTTTTAGTTTTAGTTTCCAGTCCCTATTTTCTTTTTAATAAAAAAAAAAATACAAAATTTAAAAATAATAAATTTATTATTTGTAATAAAAATTTTAATTCTCAAATTTATGTCTAACAAATTTAATTTTATATCTAAGCTTATTAAATATTTTAGGCCTAAGTTTAAAAGATCATAAATTAAATTTATAATTTAATTATTAAATAAAGAAACATATAATTACATTGAGTAATTATTTATTTTTTAATAAAAAAAAAAGCATACACGTTTAAAATTTTAAGTTAAAAATATAAAAAAATTATACAAAAATAATTTTTTAAATAAAATTAAGAGACATTTTTGAAAAATAAGAATAAATTCAAACCACCTTAGCTTGTTTTATAATTATTTGGTTTTTTATTTTGAGAATTATTTTTGTTTTTTTACCATTTTCCACTAAGAATTTCATGTTTATTAAGAAAATATTTTATTTTTTAGTTAAATTTCAAAAAAAAAAAAAAAAAAAAAATCATCAGGATAATATGTATGAAATGATTTCGTAATTTTCAATGACAAATTTGATTAAACATACAGAACCCATATGGGAAAGGAGGGGATCCCTGCGGTTCAAGCAGTGGCTCAGCCATATCAGTGGCCGCCAACATGGCGGCGGTGTCGTTAGGAACCGAGACAGATGGCTCAATTTTGTGTCCGGCCGATTACAACTCTGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGTGCCGGCGTTATCCCCATCTCTCCCCGGCAAGACACAATCGGGTATGAAAACTTGACTAAACTGAATCCTTCAAGTTCTTCTGTACTTTTTCTTCCAGTGGTCTTCTTGCTTATAAAAGATGAAGTCGATCCTAGGTTATTGATGGGACCCACGTCGACTGATTTAGAGAATGATCATGGGTTTATAAAATCAAGGAATACTCTCGAGCTTATGCTCAAAATGGACAATATGATACCATTATGGAGAGTCGTGAGTCGTGATTCCTAACCAGGACATCGAAATTGAGCTTAAACTGTTAGTTTTCCAAACACAGTATGAGTTGCACAGTGTCTTACATAAGGGGATGGGGGGGGATTGACAGGCCCATATGCAGGACAGTTTCTGATGCTGTTTATGTTCTTGATGCTATTGTTGGGTTTGATCCAATGGACTCTGAGGCAACCAAGGTTGGATCTCAATTCATTCCTTCTGGTGGTTATAAGCAATTTCTGAAGAGAGATGGGCTTACAGGAAAACGATTGGGCATTGTTAGGCATCCCTTTTCTGATTTGTATGCCAATGGATCCAAGGCCATTCAAACCTTTGAGCATCATGTCAAGCTGCTAAGGTATAACTTCATTGTTAACTCTTTTTTCTCCTTCGGCCCACAGATGATTTCATTTCTTACTGCTGAGAAATTTAAAGAGGGATCTTAGCAATGAGCTAGTGCATTTTTTTAGTTCTTGAAGTCCATTTCTTAATAAACTGTCAGATCCCACGTCAGTTGGGGAAGATAACGAAACATTATTTATAAGGGTGTGGAAATCTTTCTCTAACAGGGTGGATTGTGAGATCCCACTTCGGTTGAGGAGGAAAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTCCCTACCAGGGTGAATTGTGAGATCCCACTTCGGTTGAGGAGGAGAACAAAACATTCTTTATAAGGGTGTGGAAACCTTTCTCTACTAGGGTGGATTGTGAGATCCCACTTCGGTTGAGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTCCCTACCAGGGTGGATTGTGAGATCCCACTTCGGTTGAGGAGGAGAACAAAACATTATTTACAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAACCTTGAGGGGATGCTCGAAAGGGAAAACCCAAAAAAGACAATATCTGATAGCGGTGATTTTGGGATGTTACATAAACTAAAACCTTTTTTGGAACTTTCATAAAAGTAACGATTTAGTCTCTAAACTTAGTTTTGTTGCTTGACCTAGTCTTATTTAAGTTTTGGATGCCACTTGAACTGTGTTTAGTGAAATATTGTGTTTGTACTGAGTTTGACTCTTTCTTAAAATTGAAGGAAAAGTGGAGCAACCATTGTAGACAACCTTCAGATATCAAATGTAGCTGCAATTCTGAATCCTTATGAAAGTGGTGAATTAGTTGCAATGATAGCTGAGTTCAAGCTCACCATAAACGATTACCTCAAGAACCTGATCCAGAGTCCAGTTAGGTCTCTAGCTGACATTATTGCCTTCAACAACAACCATCCTGAACTGGTAAGACCCGTCTCGTAATAATCTAACCCGTCTCATAATAATCGTTAGATGTGAGTTCACAAATTTGCATGTCTCCCAATAATCACTAGATTTTGTAATTTCTCCAACCTTACGGGGATCTTTTTAATAGCCCAAGCACATTGCTAGCAAGTAAGAGGTTTCCACACCCTTATAAAGAATGCTTTGTTCCCCTCTCCAACCGATGTAGGATCTCACAATCCACCCCCTTGGTGCCCAGCGTCCTCACTGGCACTTGTTTCCCTCTTCATTCGATGTGGGATCTCACAATCCACCCCCTTTCGAGATTCAGCGTCCTCGTTGGCACACTGCTCGGTGCCTAGCTTTGATACCCTTTGTAATAGCCCAAGTCCATAGCTAGCAGGTATTGTCCGTTTCAACCCGTTACGTATCGCTGTCAGCCTCACGATTTTAAGAGAGATTTCTACACTCCTCACGATTTTAAGAGAGATTTGTACACTCTTATAAAAAATGCTTCGTCCCCTCTCCAACCGACGTGAGATCTCACAATCTTTGTGGGGGTTGGGCCGAATGGTCTCGTGGGATAAAATAAGAGATTGATATAGCTTTTTTGCATTGCAGGAAAACATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCCGAGCAAACCGAGGGGATCGGAGAGGCAGAGAAGGCAGCGATTAGTATGATGTCCAACTTATCAAGAGATGGATTTGAGGAAATGATGAAGACCTATAATCTGGATTCAATGGTAACATTAGGGACTGGAGCCGAAACTGTTCTAGCTATTGGGGGATACCCTGCCATCTCTGTCCCTGCTGGATACGAAGGAAACGGAGAGCCGTTTGGCGTCCTCTTTTCGGGTCTTAAGGGGACCGAACCGAAGCTGATCGAGATCGCTTATGCATACGAACAAGCCACCATGGTACGAAGGCCTCCTCCTCTTCTTTCTGACTTGTCAAGTTTGTGA

mRNA sequence

ATGTCAACTTTCCAGGATCCAATCCTTTTCCTCTACAAATCCAGACAGTCCCAATCTCCGTCCATACGCTCCTACCGCCGCCGCCGTCGCCGCCACCATCCAAAAATGAACATCATCTTCTTCCTCTCAGTTTTCTTACTCTTCGTCGGAGCCAACAGTTCTCAATTTCCGATCGACGAAGCAACCATCGCCGAAATCCAACACGCTTTCTCCCAAAACAAGCTCACCTCCAGAGAACTTCTCGATCACTACCTGAACAAGATCGATTTTCTTAATCCAGTGCTTAGAAGCGTTCTCGAAGTGAATCCTGATGCGAGAGCCCAAGCGGAAGCCGCCGATCGAGAGAGGGAGCTCGCTGGAGGAAAAGGCCTTGGCGAACTCCATGGAATTCCAGTGCTGCTCAAGGATTCAATTGGAACCAAGGATCGGCTCAATACCACGGCCGGTTCCTTCGCATTGTTAGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTCCACCGCCTAAGGAACGCCGGTGCGGTGATTTTGGGGAAAACTTCGCTCACTGAGTGGTATGGGTCTCGGTCTCTGCAGATTCCACATGGCTGGTGCGCTCGCGGCGGTCAAGCCCTGAACCCATATGGGAAAGGAGGGGATCCCTGCGGTTCAAGCAGTGGCTCAGCCATATCAGTGGCCGCCAACATGGCGGCGGTGTCGTTAGGAACCGAGACAGATGGCTCAATTTTGTGTCCGGCCGATTACAACTCTGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGTGCCGGCGTTATCCCCATCTCTCCCCGGCAAGACACAATCGGGCCCATATGCAGGACAGTTTCTGATGCTGTTTATGTTCTTGATGCTATTGTTGGGTTTGATCCAATGGACTCTGAGGCAACCAAGGTTGGATCTCAATTCATTCCTTCTGGTGGTTATAAGCAATTTCTGAAGAGAGATGGGCTTACAGGAAAACGATTGGGCATTGTTAGGCATCCCTTTTCTGATTTGTATGCCAATGGATCCAAGGCCATTCAAACCTTTGAGCATCATGTCAAGCTGCTAAGGAAAAGTGGAGCAACCATTGTAGACAACCTTCAGATATCAAATGTAGCTGCAATTCTGAATCCTTATGAAAGTGGTGAATTAGTTGCAATGATAGCTGAGTTCAAGCTCACCATAAACGATTACCTCAAGAACCTGATCCAGAGTCCAGTTAGGTCTCTAGCTGACATTATTGCCTTCAACAACAACCATCCTGAACTGGAAAACATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCCGAGCAAACCGAGGGGATCGGAGAGGCAGAGAAGGCAGCGATTAGTATGATGTCCAACTTATCAAGAGATGGATTTGAGGAAATGATGAAGACCTATAATCTGGATTCAATGGTAACATTAGGGACTGGAGCCGAAACTGTTCTAGCTATTGGGGGATACCCTGCCATCTCTGTCCCTGCTGGATACGAAGGAAACGGAGAGCCGTTTGGCGTCCTCTTTTCGGGTCTTAAGGGGACCGAACCGAAGCTGATCGAGATCGCTTATGCATACGAACAAGCCACCATGGTACGAAGGCCTCCTCCTCTTCTTTCTGACTTGTCAAGTTTGTGA

Coding sequence (CDS)

ATGTCAACTTTCCAGGATCCAATCCTTTTCCTCTACAAATCCAGACAGTCCCAATCTCCGTCCATACGCTCCTACCGCCGCCGCCGTCGCCGCCACCATCCAAAAATGAACATCATCTTCTTCCTCTCAGTTTTCTTACTCTTCGTCGGAGCCAACAGTTCTCAATTTCCGATCGACGAAGCAACCATCGCCGAAATCCAACACGCTTTCTCCCAAAACAAGCTCACCTCCAGAGAACTTCTCGATCACTACCTGAACAAGATCGATTTTCTTAATCCAGTGCTTAGAAGCGTTCTCGAAGTGAATCCTGATGCGAGAGCCCAAGCGGAAGCCGCCGATCGAGAGAGGGAGCTCGCTGGAGGAAAAGGCCTTGGCGAACTCCATGGAATTCCAGTGCTGCTCAAGGATTCAATTGGAACCAAGGATCGGCTCAATACCACGGCCGGTTCCTTCGCATTGTTAGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTCCACCGCCTAAGGAACGCCGGTGCGGTGATTTTGGGGAAAACTTCGCTCACTGAGTGGTATGGGTCTCGGTCTCTGCAGATTCCACATGGCTGGTGCGCTCGCGGCGGTCAAGCCCTGAACCCATATGGGAAAGGAGGGGATCCCTGCGGTTCAAGCAGTGGCTCAGCCATATCAGTGGCCGCCAACATGGCGGCGGTGTCGTTAGGAACCGAGACAGATGGCTCAATTTTGTGTCCGGCCGATTACAACTCTGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGTGCCGGCGTTATCCCCATCTCTCCCCGGCAAGACACAATCGGGCCCATATGCAGGACAGTTTCTGATGCTGTTTATGTTCTTGATGCTATTGTTGGGTTTGATCCAATGGACTCTGAGGCAACCAAGGTTGGATCTCAATTCATTCCTTCTGGTGGTTATAAGCAATTTCTGAAGAGAGATGGGCTTACAGGAAAACGATTGGGCATTGTTAGGCATCCCTTTTCTGATTTGTATGCCAATGGATCCAAGGCCATTCAAACCTTTGAGCATCATGTCAAGCTGCTAAGGAAAAGTGGAGCAACCATTGTAGACAACCTTCAGATATCAAATGTAGCTGCAATTCTGAATCCTTATGAAAGTGGTGAATTAGTTGCAATGATAGCTGAGTTCAAGCTCACCATAAACGATTACCTCAAGAACCTGATCCAGAGTCCAGTTAGGTCTCTAGCTGACATTATTGCCTTCAACAACAACCATCCTGAACTGGAAAACATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCCGAGCAAACCGAGGGGATCGGAGAGGCAGAGAAGGCAGCGATTAGTATGATGTCCAACTTATCAAGAGATGGATTTGAGGAAATGATGAAGACCTATAATCTGGATTCAATGGTAACATTAGGGACTGGAGCCGAAACTGTTCTAGCTATTGGGGGATACCCTGCCATCTCTGTCCCTGCTGGATACGAAGGAAACGGAGAGCCGTTTGGCGTCCTCTTTTCGGGTCTTAAGGGGACCGAACCGAAGCTGATCGAGATCGCTTATGCATACGAACAAGCCACCATGGTACGAAGGCCTCCTCCTCTTCTTTCTGACTTGTCAAGTTTGTGA

Protein sequence

MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL
Homology
BLAST of Csor.00g264080 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.2e-162
Identity = 296/490 (60.41%), Postives = 370/490 (75.51%), Query Frame = 0

Query: 53  SSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAA 112
           SS F I EATI +I+ AF++ +LTS++L++ YL  I  LNP+L +V+E NPDA  QAE A
Sbjct: 35  SSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIA 94

Query: 113 DRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAG 172
           DRER+L     L  LHG+PVLLKDSI TKD+LNTTAGSFALLGSVV RDA VV RLR +G
Sbjct: 95  DRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESG 154

Query: 173 AVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVS 232
           AVILGK SL+EW   RS  IP GW ARG Q  NPY    +P GSSSGSAISV AN+ AVS
Sbjct: 155 AVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYVLSANPSGSSSGSAISVTANLVAVS 214

Query: 233 LGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAI 292
           LGTETDGSIL PA  NSVVGIKP+VGLTSRAGV+PIS RQD+IGPICRTVSDAV++LDAI
Sbjct: 215 LGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAI 274

Query: 293 VGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEH 352
           VG+DP+D EATK  S+FIP GGYKQFL   GL GKRLGIV            K     +H
Sbjct: 275 VGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIV-----------MKHSSLLDH 334

Query: 353 HVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLA 412
           H+K LR+ GA +++NL I N+  I+   +SGE +A++AEFK+++N YLK L++SPVRSLA
Sbjct: 335 HIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLA 394

Query: 413 DIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNL 472
           D+IA+N    E E +KE+GQ+ FL +E T G+GE EK A+  M  LSR+G E++++   L
Sbjct: 395 DVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKL 454

Query: 473 DSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQAT 532
           D++VTLG+   +VLAIGGYP I+VPAGY+  G P+G+ F GL+ +EPKLIEIA+A+EQAT
Sbjct: 455 DAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQAT 512

Query: 533 MVRRPPPLLS 543
           ++R+PP  ++
Sbjct: 515 LIRKPPKFIA 512

BLAST of Csor.00g264080 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.6e-79
Identity = 198/510 (38.82%), Postives = 274/510 (53.73%), Query Frame = 0

Query: 58  IDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERE 117
           +++ATI ++Q+      LTS +++  YL++   +NP +  +L++NPD    A   D ER 
Sbjct: 66  LEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER- 125

Query: 118 LAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILG 177
            A G   G LHGIP ++KD+  TKD+++TTAGS+ALLGS+VPRDA VV +LR AGAV+ G
Sbjct: 126 -ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFG 185

Query: 178 KTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTET 237
             +L+EW   RS     G+ ARGGQ+  P+    +P GSSSGSAISVA+NM A +LGTET
Sbjct: 186 HATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTET 245

Query: 238 DGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDP 297
           DGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAVYV  ++ G D 
Sbjct: 246 DGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGIDE 305

Query: 298 MDSEATKVGSQFIPSGGYKQFL-KRDGLTGKRLGIVRHPFSDLYANG-SKAIQTFEHHVK 357
            D        +    G Y +FL  +  L G R G+   P+  L+ N  +  I      VK
Sbjct: 306 NDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLWQNAKADEIDRLLEVVK 365

Query: 358 LLRKSGATIVDNLQISNVAAILNP---YESG-----ELVAMIAEFKLTINDYLKNLIQSP 417
            + ++GA + +N    N+  I N    +E G     E   +  +F   I  YL  +  + 
Sbjct: 366 QIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTE 425

Query: 418 VRSLADIIAFNNNHPELENMK-------EYGQDAFLLSEQTEGI-GEAEKAAISMMSNLS 477
           + SL DI+ +NN +   E  K         GQD FL S +  G+  E    A+  +   S
Sbjct: 426 IHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTS 485

Query: 478 RDGFEEMMKTY----NLDSMVTLGTGAETVLAI-------GGYPAISVPAGYEGNGEPFG 537
           +D   +    Y      DS +  G    +  +I        GYP I++P G + NG PFG
Sbjct: 486 QDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFG 545

Query: 538 VLFSGLKGTEPKLIEIAYAYEQATMVRRPP 539
           +        EP+LI+   A E     +  P
Sbjct: 546 LGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of Csor.00g264080 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.7e-66
Identity = 167/491 (34.01%), Postives = 262/491 (53.36%), Query Frame = 0

Query: 66  IQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAGGKGLG 125
           +Q  + Q  +    ++  Y+ +I  +N  +R+V E+NPDA   A+  D ER++  GK  G
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKM--GKLRG 153

Query: 126 ELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWY 185
            LHG+P+++K++I T D++++TAGS+A+ G+    DATV  +LR AG VI+GK+  ++W 
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 186 GSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA 245
             RSL   +GW A GGQ    Y K  DP GSSSGS ++    +A  +LGTET GSI+ PA
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 246 DYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKV 305
           D +++VG+KPTVGLTSR  V+PIS RQDT+GP+ R+V DA Y+L  I G D  D+  + +
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSAI 333

Query: 306 GSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIV 365
               IP   Y +    + L GKR+G+ R+    ++ +    +  F   + +++K+GA IV
Sbjct: 334 PFDTIPD--YVKACDINALKGKRIGVPRNVIK-IFGSPQTVVDQFNQALAVMKKAGAIIV 393

Query: 366 DNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSP--VRSLADIIAFNNNHPE 425
           +N   ++ A         ++  + A+    +  + K L  +P  +  L  +  F  +H  
Sbjct: 394 ENTDFTSFAEFAQSPIPDDI--LYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH-R 453

Query: 426 LENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFE----EMMKTYNLDSMVTLG 485
           LE         + ++ Q +GI   +     M     + G E      ++ + LD+ V   
Sbjct: 454 LEEYPSRDTARWDIALQ-KGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPT 513

Query: 486 TGAETVLAIGGYPAISVPAGY---------------EGNGEPFGVLFSGLKGTEPKLIEI 536
             +  + A+ G P I+VP G                 G G P G+ F G   +E KLI +
Sbjct: 514 DLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGL 573

BLAST of Csor.00g264080 vs. ExPASy Swiss-Prot
Match: Q0I9N6 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Synechococcus sp. (strain CC9311) OX=64471 GN=gatA PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.2e-44
Identity = 161/502 (32.07%), Postives = 238/502 (47.41%), Query Frame = 0

Query: 63  IAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPD-ARAQAEAADRERELAGG 122
           IAE +      ++++REL DH+L +I  ++  + + LEV  D ARA A+  D  R  A G
Sbjct: 3   IAEWRQQLESGEVSARELTDHHLARIKAVDSSVHAFLEVTADRARADADRLDEAR--AAG 62

Query: 123 KGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSL 182
           + L  L G+P+ +KD++ TK  + TT+ S  L   V P ++TV  RL  +GAV++GKT+L
Sbjct: 63  EDLPPLAGVPIAIKDNLCTKG-IRTTSSSRMLESFVPPYESTVTDRLWRSGAVLIGKTNL 122

Query: 183 TEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSI 242
            E+    S +      +  G   NP+  G  P GSS GSA +VAA     SLG++T GSI
Sbjct: 123 DEFAMGGSTE-----TSAFGPTANPWNTGYVPGGSSGGSAAAVAAGECMASLGSDTGGSI 182

Query: 243 LCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSE 302
             PA +  VVG+KPT G  SR G++  +   D +GP   +VSDA  +L AI G DP DS 
Sbjct: 183 RQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQVGPFATSVSDAAELLQAIAGEDPRDST 242

Query: 303 ATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKSG 362
             K      P   Y++ L R  ++G R+G+VR  F D      +   +     +LL+  G
Sbjct: 243 CLKA-----PVPNYREVLGR-SVSGLRIGVVRECF-DQEGIDPQVKASVLAAAELLQSLG 302

Query: 363 ATIVDNL--QISNVAAILNPYESGELVAMIAEFKLTINDYLK-NLIQSPVRSLADIIAFN 422
           A +VD    + ++  A        E  A +A +     D +K         SLA + A  
Sbjct: 303 AELVDVSCPRFNDGIATYYVIAPSEASANLARY-----DGVKYGFRAEDASSLASMTA-- 362

Query: 423 NNHPELENMKEYGQDAFLLSEQ--TEGIGEAEKAAISMMSNLSRDGFEEMMKTYNL---- 482
                 E      Q   L+     + G  +A       +  L R  FE    + ++    
Sbjct: 363 --RSRTEGFGSEVQRRILIGTYALSAGYVDAYYRKAQQVRTLIRRDFETAFASVDVLLTP 422

Query: 483 ---DSMVTLGTGAETVLA------------IGGYPAISVPAGYEGNGEPFGVLFSGLKGT 538
               +    G  A+  LA            + G PAI+VP G++  G P GV   G    
Sbjct: 423 TAPSTAFAAGAHADDPLAMYLADLLTIPANLAGLPAINVPCGFDSEGLPIGVQLIGNVLE 480

BLAST of Csor.00g264080 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.4e-43
Identity = 159/492 (32.32%), Postives = 236/492 (47.97%), Query Frame = 0

Query: 62  TIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPD-ARAQAEAADRERELAG 121
           +I ++       + T+ E+   +L +I  L P ++S L + PD A AQA+  D   ++A 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVD--EKIAR 62

Query: 122 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 181
           G+ L  L GIP+ LKD++ TK  + TT  S  L   V P ++TV  +LR+ GAVI+GKT+
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 182 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 241
           L E+    S +   G+        NP+     P GSS GSA +VAA    V+LG++T GS
Sbjct: 123 LDEFAMGSSTE-NSGYHV----TANPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGGS 182

Query: 242 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 301
           I  PA +  VVG+KPT GL SR G++  +   D IGP  RTV DA  +L AI G+DP DS
Sbjct: 183 IRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQDS 242

Query: 302 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 361
            +       +P   Y QFLK   L G ++G+++  F +         +     +  L+  
Sbjct: 243 TSLN-----LPIPDYSQFLK-TSLKGLKIGVIKETFGE--GLDQVVAEAVNQALAQLKAL 302

Query: 362 GATIVDNLQIS--------NVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLA 421
           GATI    +IS            I+ P E+   +A     K  I +   +LI    ++ A
Sbjct: 303 GATI---KEISCPRFRYGLPTYYIIAPSEASANLARYDGVKYGIREDADSLIDMYTKTRA 362

Query: 422 DIIAFNNNHPELENMKEYG-----QDAFLLSEQ------TEGIGEAEKAAISMMSNLS-R 481
               F         +  Y       DA+ L  Q       E    A ++   ++S  S  
Sbjct: 363 K--GFGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAFQSVDVLVSPTSPT 422

Query: 482 DGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPK 533
             F+   KT +  SM  L       + + G P +S+P G++G G P G+   G    E +
Sbjct: 423 TAFKAGEKTADPLSMY-LSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNVLREDQ 472

BLAST of Csor.00g264080 vs. NCBI nr
Match: KAG6589001.1 (putative amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60
           MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE
Sbjct: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60

Query: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120
           ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG
Sbjct: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120

Query: 121 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180
           GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS
Sbjct: 121 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180

Query: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240
           LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS
Sbjct: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240

Query: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300
           ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS
Sbjct: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300

Query: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360
           EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS
Sbjct: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360

Query: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420
           GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN
Sbjct: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420

Query: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480
           HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT
Sbjct: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480

Query: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540
           GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL
Sbjct: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540

Query: 541 LSDLSSL 547
           LSDLSSL
Sbjct: 541 LSDLSSL 547

BLAST of Csor.00g264080 vs. NCBI nr
Match: KAG7015266.1 (hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1058 bits (2736), Expect = 0.0
Identity = 545/547 (99.63%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60
           MSTFQDPILFL KSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE
Sbjct: 1   MSTFQDPILFLDKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60

Query: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120
           ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG
Sbjct: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120

Query: 121 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180
           GK LGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS
Sbjct: 121 GKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180

Query: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240
           LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS
Sbjct: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240

Query: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300
           ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS
Sbjct: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300

Query: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360
           EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS
Sbjct: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360

Query: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420
           GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN
Sbjct: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420

Query: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480
           HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT
Sbjct: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480

Query: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540
           GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL
Sbjct: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540

Query: 541 LSDLSSL 547
           LSDLSSL
Sbjct: 541 LSDLSSL 547

BLAST of Csor.00g264080 vs. NCBI nr
Match: XP_022928333.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 542/547 (99.09%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60
           MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNI+FFLSVFLLFVGANSSQFPIDE
Sbjct: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIVFFLSVFLLFVGANSSQFPIDE 60

Query: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120
           ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAE ADRERELAG
Sbjct: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAETADRERELAG 120

Query: 121 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180
           GK LGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS
Sbjct: 121 GKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180

Query: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240
           LTEWYGSRSL+IPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS
Sbjct: 181 LTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240

Query: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300
           ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS
Sbjct: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300

Query: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360
           EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS
Sbjct: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360

Query: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420
           GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN
Sbjct: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420

Query: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480
           HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLD+MVTLGT
Sbjct: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDAMVTLGT 480

Query: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540
           GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL
Sbjct: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540

Query: 541 LSDLSSL 547
           LSDLSSL
Sbjct: 541 LSDLSSL 547

BLAST of Csor.00g264080 vs. NCBI nr
Match: XP_023529660.1 (probable amidase At4g34880 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 530/542 (97.79%), Postives = 537/542 (99.08%), Query Frame = 0

Query: 6   DPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDEATIAE 65
           DPILFLYKSRQSQSPSIRSYRRRRRRH+PKMNI+FFLS FLLF+GANSSQFPIDEATIAE
Sbjct: 13  DPILFLYKSRQSQSPSIRSYRRRRRRHYPKMNIVFFLSAFLLFIGANSSQFPIDEATIAE 72

Query: 66  IQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAGGKGLG 125
           IQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRER+LAGGK LG
Sbjct: 73  IQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERDLAGGKALG 132

Query: 126 ELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWY 185
           ELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWY
Sbjct: 133 ELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWY 192

Query: 186 GSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA 245
           GSRSL+IPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA
Sbjct: 193 GSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA 252

Query: 246 DYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKV 305
           DYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKV
Sbjct: 253 DYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKV 312

Query: 306 GSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIV 365
           GSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGS AIQ+FEHHVKLLRKSGATIV
Sbjct: 313 GSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSTAIQSFEHHVKLLRKSGATIV 372

Query: 366 DNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELE 425
           DNLQISNVA I NPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELE
Sbjct: 373 DNLQISNVAVIQNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELE 432

Query: 426 NMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGTGAETV 485
           NMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLD+MVTLGTGAETV
Sbjct: 433 NMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDAMVTLGTGAETV 492

Query: 486 LAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLS 545
           LAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLS
Sbjct: 493 LAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLS 552

Query: 546 SL 547
           SL
Sbjct: 553 SL 554

BLAST of Csor.00g264080 vs. NCBI nr
Match: XP_022989045.1 (putative amidase C869.01 [Cucurbita maxima] >XP_022989046.1 putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 495/512 (96.68%), Postives = 504/512 (98.44%), Query Frame = 0

Query: 36  MNIIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95
           MNI+FFLS FL F+GANSSQFPIDEAT+AEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL
Sbjct: 1   MNIVFFLSAFLFFIGANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 60

Query: 96  RSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155
           +SVLEVNPDARAQAEAADRERELAGGK LGELHGIPVLLKDSIGTKDRLNTTAGSFALLG
Sbjct: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 120

Query: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCG 215
           SVVPRDA VVHRLRNAGAVILGKTSLTEWYGSRS++IPHGWCARGGQALNPYGKGGDPCG
Sbjct: 121 SVVPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKGGDPCG 180

Query: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240

Query: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335
           GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP
Sbjct: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 300

Query: 336 FSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT 395
           FSDLYAN S AIQ+FEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT
Sbjct: 301 FSDLYANESTAIQSFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT 360

Query: 396 INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM 455
           INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM
Sbjct: 361 INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM 420

Query: 456 SNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLK 515
            NLSR GFEEMMKTYNLD+MVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LFSGLK
Sbjct: 421 ENLSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFSGLK 480

Query: 516 GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547
           GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL
Sbjct: 481 GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 512

BLAST of Csor.00g264080 vs. ExPASy TrEMBL
Match: A0A6J1ENQ3 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 542/547 (99.09%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIIFFLSVFLLFVGANSSQFPIDE 60
           MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNI+FFLSVFLLFVGANSSQFPIDE
Sbjct: 1   MSTFQDPILFLYKSRQSQSPSIRSYRRRRRRHHPKMNIVFFLSVFLLFVGANSSQFPIDE 60

Query: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELAG 120
           ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAE ADRERELAG
Sbjct: 61  ATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAETADRERELAG 120

Query: 121 GKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180
           GK LGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS
Sbjct: 121 GKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKTS 180

Query: 181 LTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240
           LTEWYGSRSL+IPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS
Sbjct: 181 LTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGS 240

Query: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300
           ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS
Sbjct: 241 ILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDS 300

Query: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360
           EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS
Sbjct: 301 EATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEHHVKLLRKS 360

Query: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420
           GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN
Sbjct: 361 GATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLADIIAFNNN 420

Query: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDSMVTLGT 480
           HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLD+MVTLGT
Sbjct: 421 HPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNLDAMVTLGT 480

Query: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540
           GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL
Sbjct: 481 GAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQATMVRRPPPL 540

Query: 541 LSDLSSL 547
           LSDLSSL
Sbjct: 541 LSDLSSL 547

BLAST of Csor.00g264080 vs. ExPASy TrEMBL
Match: A0A6J1JLA2 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1)

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 495/512 (96.68%), Postives = 504/512 (98.44%), Query Frame = 0

Query: 36  MNIIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95
           MNI+FFLS FL F+GANSSQFPIDEAT+AEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL
Sbjct: 1   MNIVFFLSAFLFFIGANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 60

Query: 96  RSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155
           +SVLEVNPDARAQAEAADRERELAGGK LGELHGIPVLLKDSIGTKDRLNTTAGSFALLG
Sbjct: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 120

Query: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCG 215
           SVVPRDA VVHRLRNAGAVILGKTSLTEWYGSRS++IPHGWCARGGQALNPYGKGGDPCG
Sbjct: 121 SVVPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKGGDPCG 180

Query: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240

Query: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335
           GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP
Sbjct: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 300

Query: 336 FSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT 395
           FSDLYAN S AIQ+FEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT
Sbjct: 301 FSDLYANESTAIQSFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLT 360

Query: 396 INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM 455
           INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM
Sbjct: 361 INDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMM 420

Query: 456 SNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLK 515
            NLSR GFEEMMKTYNLD+MVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LFSGLK
Sbjct: 421 ENLSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFSGLK 480

Query: 516 GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547
           GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL
Sbjct: 481 GTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 512

BLAST of Csor.00g264080 vs. ExPASy TrEMBL
Match: A0A6J1C363 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111008028 PE=4 SV=1)

HSP 1 Score: 860 bits (2221), Expect = 1.14e-311
Identity = 436/507 (86.00%), Postives = 466/507 (91.91%), Query Frame = 0

Query: 38  IIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 97
           ++FFL+ F LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61

Query: 98  VLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 157
           VLEVNPDARAQAEAADRER+LAGGK LGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121

Query: 158 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSS 217
           VPRDATVV RLR AGAVILGK SLTEWYGSRSL+IP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181

Query: 218 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 277
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241

Query: 278 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 337
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301

Query: 338 DLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 397
           DLY NGS AI TFEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361

Query: 398 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 457
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421

Query: 458 LSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 517
           LSRDGFE MMKTYNLD+MVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481

Query: 518 EPKLIEIAYAYEQATMVRRPPPLLSDL 544
           E KLIEIAYA+EQATMVRRPPPL  +L
Sbjct: 482 ETKLIEIAYAFEQATMVRRPPPLPFEL 508

BLAST of Csor.00g264080 vs. ExPASy TrEMBL
Match: A0A1S3BIN0 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1)

HSP 1 Score: 838 bits (2166), Expect = 9.27e-303
Identity = 436/516 (84.50%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 34  PKMNIIFF-LSVFLLFVG-ANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFL 93
           PKMNI+FF LS  LLF G ANSS F IDEATI EIQ+AFSQNKLTS +LLD+YLNKI  L
Sbjct: 31  PKMNIVFFFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHLL 90

Query: 94  NPVLRSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSF 153
           NPVL+SVLE+NPDARAQAEAADRER LA GK LGELHGIP+LLKD+I TKD LNTTAGSF
Sbjct: 91  NPVLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGSF 150

Query: 154 ALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGG 213
           ALLGSVVPRDATVV RLRNAGAVILGKTSLTEW+ SRS +IP+GWCARGGQA+NPYGKGG
Sbjct: 151 ALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKGG 210

Query: 214 DPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPR 273
           DPCGSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPR
Sbjct: 211 DPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPR 270

Query: 274 QDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGI 333
           QDTIGPICRTVSDAVYVL+AIVGFDPMD E TK  SQFIPSGGYKQFL++DGL GKRLGI
Sbjct: 271 QDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKDGLKGKRLGI 330

Query: 334 VRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAE 393
           VRHPFSDLY+N S AI TFE H+ LLRK GATIVDNLQISNV  ILNPYESGE +A+IAE
Sbjct: 331 VRHPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIAE 390

Query: 394 FKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAA 453
           FKL +NDYLK LIQSPVRSLADII+FNN HPELENMKEYGQDAFLLSEQT GIGE EK A
Sbjct: 391 FKLAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKEA 450

Query: 454 ISMMSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLF 513
           IS M NLSR+GFEE+MK  NLD+MVT+GTG E+VLAIGGYP ISVPAGYE NGEPFG+LF
Sbjct: 451 ISRMMNLSRNGFEELMKGNNLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGILF 510

Query: 514 SGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547
            GLKG+EPKLIEIAYAYEQATMVR PP LLS +SSL
Sbjct: 511 GGLKGSEPKLIEIAYAYEQATMVREPP-LLSRISSL 545

BLAST of Csor.00g264080 vs. ExPASy TrEMBL
Match: A0A5A7U802 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00790 PE=4 SV=1)

HSP 1 Score: 830 bits (2144), Expect = 6.26e-300
Identity = 433/514 (84.24%), Postives = 462/514 (89.88%), Query Frame = 0

Query: 36  MNIIFF-LSVFLLFVG-ANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNP 95
           MNI+FF LS  LLF G ANSS F IDEATI EIQ+AFSQNKLTS +LLD+YLNKI  LNP
Sbjct: 1   MNIVFFFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHLLNP 60

Query: 96  VLRSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFAL 155
           VL+SVLE+NPDARAQAEAADRER LA GK LGELHGIP+LLKD+I TKD LNTTAGSFAL
Sbjct: 61  VLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGSFAL 120

Query: 156 LGSVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDP 215
           LGSVVPRDATVV RLRNAGAVILGKTSLTEW+ SRS +IP+GWCARGGQA+NPYGKGGDP
Sbjct: 121 LGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKGGDP 180

Query: 216 CGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQD 275
           CGSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQD
Sbjct: 181 CGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQD 240

Query: 276 TIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVR 335
           TIGPICRTVSDAVYVL+AIVGFDPMD E TK  SQFIPSGGYKQFL+ DGL GKRLGIVR
Sbjct: 241 TIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRIDGLKGKRLGIVR 300

Query: 336 HPFSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFK 395
           HPFSDLY+N S AI TFE H+ LLRK GATIVDNLQISNV  ILNPYESGE +A+IAEFK
Sbjct: 301 HPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIAEFK 360

Query: 396 LTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAIS 455
           L +NDYLK LIQSPVRSLADII+FNN HPELENMKEYGQDAFLLSEQT GIGE EK AIS
Sbjct: 361 LAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKEAIS 420

Query: 456 MMSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSG 515
            M NLSR+GFEE+MK  +LD+MVT+GTG E+VLAIGGYP ISVPAGYE NGEPFG+LF G
Sbjct: 421 RMMNLSRNGFEELMKGNHLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGILFGG 480

Query: 516 LKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547
           LKG+EPKLIEIAYAYEQATMVR PP LLS +SSL
Sbjct: 481 LKGSEPKLIEIAYAYEQATMVREPP-LLSRISSL 513

BLAST of Csor.00g264080 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 495.0 bits (1273), Expect = 7.9e-140
Identity = 262/490 (53.47%), Postives = 334/490 (68.16%), Query Frame = 0

Query: 53  SSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAA 112
           SS F I EATI +I+ AF++ +LTS++L++ YL  I  LNP+L +V+E NPDA  QAE A
Sbjct: 35  SSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIA 94

Query: 113 DRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAG 172
           DRER+L     L  LHG+PVLLKDSI TKD+LNTTAGSFALLGSVV RDA VV RLR +G
Sbjct: 95  DRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESG 154

Query: 173 AVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVS 232
           AVILGK SL+EW   RS  IP GW A                                  
Sbjct: 155 AVILGKASLSEWAHFRSFSIPDGWSA---------------------------------- 214

Query: 233 LGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAI 292
                          NSVVGIKP+VGLTSRAGV+PIS RQD+IGPICRTVSDAV++LDAI
Sbjct: 215 ------------PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAI 274

Query: 293 VGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFSDLYANGSKAIQTFEH 352
           VG+DP+D EATK  S+FIP GGYKQFL   GL GKRLGIV            K     +H
Sbjct: 275 VGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIV-----------MKHSSLLDH 334

Query: 353 HVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTINDYLKNLIQSPVRSLA 412
           H+K LR+ GA +++NL I N+  I+   +SGE +A++AEFK+++N YLK L++SPVRSLA
Sbjct: 335 HIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLA 394

Query: 413 DIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSNLSRDGFEEMMKTYNL 472
           D+IA+N    E E +KE+GQ+ FL +E T G+GE EK A+  M  LSR+G E++++   L
Sbjct: 395 DVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKL 454

Query: 473 DSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGTEPKLIEIAYAYEQAT 532
           D++VTLG+   +VLAIGGYP I+VPAGY+  G P+G+ F GL+ +EPKLIEIA+A+EQAT
Sbjct: 455 DAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQAT 466

Query: 533 MVRRPPPLLS 543
           ++R+PP  ++
Sbjct: 515 LIRKPPKFIA 466

BLAST of Csor.00g264080 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 131.0 bits (328), Expect = 3.0e-30
Identity = 101/304 (33.22%), Postives = 157/304 (51.64%), Query Frame = 0

Query: 60  EATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVNPDARAQAEAADRERELA 119
           ++ I   + +    + T+ E+   YL++I    P L+  L V+ +    A+  D  + +A
Sbjct: 43  QSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID--QRIA 102

Query: 120 GGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHRLRNAGAVILGKT 179
            G+ LG L G+ + +KD+I T+  + +TA S  L     P DAT V +++  G +++GKT
Sbjct: 103 KGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKT 162

Query: 180 SLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDG 239
           ++ E +G  S      +        NP+     P GSS GSA +VAA    VSLG++T G
Sbjct: 163 NMDE-FGMGSTTEASAFQV----TANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGG 222

Query: 240 SILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMD 299
           S+  PA +  VVG+KPT G  SR G++  +   D IG    TV+DA  +L AI G+D  D
Sbjct: 223 SVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFD 282

Query: 300 SEATKVGSQFIPSGGYKQFLKRD-----GLTGKRLGIVRHPFSDLYANGSK-AIQTFEHH 358
           S ++K   Q +P     QFL  D      L G ++GI+R    D   +G + A Q    H
Sbjct: 283 STSSK---QDVPE-FQSQFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASH 334

BLAST of Csor.00g264080 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-27
Identity = 87/263 (33.08%), Postives = 132/263 (50.19%), Query Frame = 0

Query: 48  FVGANSSQFPIDEATIA-----EIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVN 107
           +  A+  + P DE  IA     E+       ++TS+EL+  YL ++   N VL +V+   
Sbjct: 185 YPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYT 244

Query: 108 PDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDA 167
            +  A  +A + +  L+ G  LG LHGIP  LKD +       TT GS +     +  +A
Sbjct: 245 EEL-AYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEA 304

Query: 168 TVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAI 227
            V  RL+ +GAV++ K        + S+     W   GG+  NP+       GSS+G A 
Sbjct: 305 WVYKRLKASGAVLVAKLV------TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPAA 364

Query: 228 SVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTV 287
           S +A M   ++G+ET GS+  PA    +  ++PT G   R GV+ IS   D +GP CRT 
Sbjct: 365 STSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTA 424

Query: 288 SDAVYVLDAIVGFDPMDSEATKV 306
           +D   +LDAI G DP D  + ++
Sbjct: 425 ADCAVILDAIKGKDPDDLSSREI 437

BLAST of Csor.00g264080 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-24
Identity = 86/263 (32.70%), Postives = 129/263 (49.05%), Query Frame = 0

Query: 48  FVGANSSQFPIDEATIA-----EIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRSVLEVN 107
           +  A+  + P DE  IA     E+       ++TS+EL+  YL ++   N VL +V+   
Sbjct: 185 YPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYT 244

Query: 108 PDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDA 167
            +  A  +A + +  L+ G  LG LHGIP  LKD +       TT GS +     +  +A
Sbjct: 245 EEL-AYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEA 304

Query: 168 TVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAI 227
            V  RL+ +GAV++ K        + S+     W   GG+  NP+       GSS+G A 
Sbjct: 305 WVYKRLKASGAVLVAKLV------TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPA- 364

Query: 228 SVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTV 287
                 A+ S G+ET GS+  PA    +  ++PT G   R GV+ IS   D +GP CRT 
Sbjct: 365 ------ASTSAGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTA 424

Query: 288 SDAVYVLDAIVGFDPMDSEATKV 306
           +D   +LDAI G DP D  + ++
Sbjct: 425 ADCAVILDAIKGKDPDDLSSREI 430

BLAST of Csor.00g264080 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 96.7 bits (239), Expect = 6.3e-20
Identity = 119/456 (26.10%), Postives = 191/456 (41.89%), Query Frame = 0

Query: 108 QAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSVVPRDATVVHR 167
           QAEA+ R  E   G  +  L GI V +KD I           ++      V +D+ VV +
Sbjct: 180 QAEASTRRFE--QGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSK 239

Query: 168 LRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAAN 227
           LR+ GA++LGK ++ E     +     G  +  G   NP+       GSSSGSA  VAA 
Sbjct: 240 LRSCGAILLGKANMHELGMGTT-----GNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAG 299

Query: 228 MAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVY 287
           + + +LGT+  GS+  P+    + G+K T G T   G +      + IGP+  ++ DA  
Sbjct: 300 LCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFL 359

Query: 288 VLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDG---LTGKRLGIVRHPFSDLYANGS 347
           V  AI+G    D    K      P     + L  +G   +   RLG     F+D+  + S
Sbjct: 360 VYAAILGSSSADRYNLKPSPPCFP-----KLLSHNGSNAIGSLRLGKYTKWFNDV--SSS 419

Query: 348 KAIQTFEHHVKLLRKS-GATIV-------DNLQISNVAAI-------LNPY-ESGELVAM 407
                 E  +KLL  + G  +V       + ++ ++V +I       L PY E+G+    
Sbjct: 420 DISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGK---- 479

Query: 408 IAEFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAE 467
               KL+ +      I     +   I A       +E      +D  ++   T G+    
Sbjct: 480 --NSKLSYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGM---- 539

Query: 468 KAAISMMSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG 527
             A  +  +  ++G   +  T +L   V           + G+PAISVP GY+  G P G
Sbjct: 540 -TAPVIPPDALKNGETNIQVTTDLMRFVLAAN-------LLGFPAISVPVGYDKEGLPIG 599

Query: 528 VLFSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDL 545
           +   G    E  ++ +A A E+   V + P +  D+
Sbjct: 600 LQIMGRPWAEATVLGLAAAVEELAPVTKKPAIFYDI 603

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7603.2e-16260.41Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY47.6e-7938.82Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C85.7e-6634.01Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
Q0I9N61.2e-4432.07Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Synechococcus sp. (strain CC931... [more]
B0JSX31.4e-4332.32Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ... [more]
Match NameE-valueIdentityDescription
KAG6589001.10.0100.00putative amidase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7015266.10.099.63hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022928333.10.099.09putative amidase C869.01 [Cucurbita moschata][more]
XP_023529660.10.097.79probable amidase At4g34880 [Cucurbita pepo subsp. pepo][more]
XP_022989045.10.096.68putative amidase C869.01 [Cucurbita maxima] >XP_022989046.1 putative amidase C86... [more]
Match NameE-valueIdentityDescription
A0A6J1ENQ30.099.09putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1[more]
A0A6J1JLA20.096.68putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1[more]
A0A6J1C3631.14e-31186.00putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111008028 PE=4 SV=... [more]
A0A1S3BIN09.27e-30384.50putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1[more]
A0A5A7U8026.26e-30084.24Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G007... [more]
Match NameE-valueIdentityDescription
AT4G34880.17.9e-14053.47Amidase family protein [more]
AT3G25660.13.0e-3033.22Amidase family protein [more]
AT5G07360.12.4e-2733.08Amidase family protein [more]
AT5G07360.21.1e-2432.70Amidase family protein [more]
AT5G64440.16.3e-2026.10fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 48..540
e-value: 1.0E-146
score: 491.9
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 58..540
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 79..521
e-value: 9.0E-74
score: 249.1
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 47..541
NoneNo IPR availablePANTHERPTHR42678:SF25C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 47..541

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g264080.m01Csor.00g264080.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane