Csor.00g260850 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g260850
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	protein RALF-like 4
Location	Csor_Chr11: 10497580 .. 10497816 (+)
RNA-Seq Expression	Csor.00g260850
Synteny	Csor.00g260850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGAAGATAGTCGTCGACAACTTTTCCAATATGGATTCGCCTATAATAAGGAAGCTCGCAGGAGGTTTTTGACCTACTATGCACTTAGTAAGAATAATATCCCTTGCGGTCGTCGTGGTACCTCCTACTATGATTGCAAGAAGCGTAGAAGGATCAATCCTTATCGTCGTGGTTGTGCTGCCATCACTGGATGTGCTCGTTCACCGATTAAACAAGTTTTATTGCCTCATTGA

mRNA sequence

ATGACTGAAGATAGTCGTCGACAACTTTTCCAATATGGATTCGCCTATAATAAGGAAGCTCGCAGGAGGTTTTTGACCTACTATGCACTTAGTAAGAATAATATCCCTTGCGGTCGTCGTGGTACCTCCTACTATGATTGCAAGAAGCGTAGAAGGATCAATCCTTATCGTCGTGGTTGTGCTGCCATCACTGGATGTGCTCGTTCACCGATTAAACAAGTTTTATTGCCTCATTGA

Coding sequence (CDS)

ATGACTGAAGATAGTCGTCGACAACTTTTCCAATATGGATTCGCCTATAATAAGGAAGCTCGCAGGAGGTTTTTGACCTACTATGCACTTAGTAAGAATAATATCCCTTGCGGTCGTCGTGGTACCTCCTACTATGATTGCAAGAAGCGTAGAAGGATCAATCCTTATCGTCGTGGTTGTGCTGCCATCACTGGATGTGCTCGTTCACCGATTAAACAAGTTTTATTGCCTCATTGA

Protein sequence

MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCARSPIKQVLLPH

Homology

[Close]

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot
Match: Q6NME6 (Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 4.2e-15
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2   TEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCA 61
           +E +RRQL          ARR +++Y AL KNN+PC RRG SYYDCKKR+R NPYRRGC+
Sbjct: 50  SETNRRQL---------AARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCS 107

Query: 62  AITGCAR 69
            IT C R
Sbjct: 110 VITHCYR 107

[Close]

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot
Match: Q9FZA0 (Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.2e-14
Identity = 40/67 (59.70%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2   TEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCA 61
           +E +RRQL +          RR++ Y AL KNN+PC RRG SYYDCKKRRR NPYRRGC+
Sbjct: 50  SETNRRQLAR---------GRRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCS 107

Query: 62  AITGCAR 69
           AIT C R
Sbjct: 110 AITHCYR 107

[Close]

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.2e-11
Identity = 29/50 (58.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCARS 70
           A RR+++Y AL +N IPC RRG SYY+C++  + NPY RGC+AIT C RS
Sbjct: 89  ATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRRS 138

[Close]

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.1e-10
Identity = 26/47 (55.32%), Postives = 36/47 (76.60%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGC 67
           A +++++Y AL KN++PC RRG SYY+CK   + NPY RGC+AIT C
Sbjct: 67  ATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRC 113

[Close]

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.2e-09
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCAR 69
           A  ++++Y AL +N +PC RRG SYY+C++  + NPY RGC+AIT C R
Sbjct: 68  ATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Csor.00g260850 vs. NCBI nr
Match: KAG6588677.1 (Protein RALF-like 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 163 bits (413), Expect = 9.21e-51
Identity = 78/78 (100.00%), Postives = 78/78 (100.00%), Query Frame = 0


Query: 1  MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
          MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 1  MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60

Query: 61 AAITGCARSPIKQVLLPH 78
          AAITGCARSPIKQVLLPH
Sbjct: 61 AAITGCARSPIKQVLLPH 78

[Close]

BLAST of Csor.00g260850 vs. NCBI nr
Match: XP_022927734.1 (protein RALF-like 4 [Cucurbita moschata])

HSP 1 Score: 142 bits (359), Expect = 6.06e-42
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 52  MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 111

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 112 AAITGCAR 119

[Close]

BLAST of Csor.00g260850 vs. NCBI nr
Match: KAG6588678.1 (Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022465.1 Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 140 bits (352), Expect = 6.25e-41
Identity = 66/68 (97.06%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           +TEDSRRQLFQYGFAYN+EARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 48  ITEDSRRQLFQYGFAYNREARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 107

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 108 AAITGCAR 115

[Close]

BLAST of Csor.00g260850 vs. NCBI nr
Match: XP_022927735.1 (protein RALF-like 4 [Cucurbita moschata])

HSP 1 Score: 140 bits (352), Expect = 7.06e-41
Identity = 66/68 (97.06%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           +TEDSRRQLFQYGFAYN+EARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 52  ITEDSRRQLFQYGFAYNREARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 111

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 112 AAITGCAR 119

[Close]

BLAST of Csor.00g260850 vs. NCBI nr
Match: KAG6588679.1 (Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022466.1 Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 138 bits (348), Expect = 2.54e-40
Identity = 65/68 (95.59%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLFQYGF YNKEAR+RFL+YYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 48  MTEDSRRQLFQYGFVYNKEARKRFLSYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 107

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 108 AAITGCAR 115

[Close]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL
Match: A0A6J1EIU0 (protein RALF-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111434547 PE=3 SV=1)

HSP 1 Score: 142 bits (359), Expect = 2.93e-42
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 52  MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 111

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 112 AAITGCAR 119

[Close]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL
Match: A0A6J1EPT5 (protein RALF-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111434548 PE=3 SV=1)

HSP 1 Score: 140 bits (352), Expect = 3.42e-41
Identity = 66/68 (97.06%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           +TEDSRRQLFQYGFAYN+EARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 52  ITEDSRRQLFQYGFAYNREARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 111

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 112 AAITGCAR 119

[Close]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL
Match: A0A6J1EIG3 (rapid alkalinization factor 23-like OS=Cucurbita moschata OX=3662 GN=LOC111434549 PE=3 SV=1)

HSP 1 Score: 138 bits (348), Expect = 1.35e-40
Identity = 65/68 (95.59%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLFQYGF YNKEAR+RFL+YYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC
Sbjct: 51  MTEDSRRQLFQYGFVYNKEARKRFLSYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 110

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 111 AAITGCAR 118

[Close]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL
Match: A0A6J1JL71 (protein RALF-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111486206 PE=3 SV=1)

HSP 1 Score: 137 bits (346), Expect = 2.72e-40
Identity = 65/68 (95.59%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLFQYGFAYN EAR+RFLTYYALSKNNIPCGRRG+SYYDCKKRRRINPYRRGC
Sbjct: 51  MTEDSRRQLFQYGFAYNTEARKRFLTYYALSKNNIPCGRRGSSYYDCKKRRRINPYRRGC 110

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 111 AAITGCAR 118

[Close]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL
Match: A0A6J1JIT9 (protein RALF-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111486203 PE=3 SV=1)

HSP 1 Score: 136 bits (343), Expect = 8.28e-40
Identity = 64/68 (94.12%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1   MTEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGC 60
           MTEDSRRQLF+YGFAYN+EAR+RFLTYYALSKNNIPCGRRG SYYDCKKRRRINPYRRGC
Sbjct: 53  MTEDSRRQLFEYGFAYNREARKRFLTYYALSKNNIPCGRRGHSYYDCKKRRRINPYRRGC 112

Query: 61  AAITGCAR 68
           AAITGCAR
Sbjct: 113 AAITGCAR 120

[Close]

BLAST of Csor.00g260850 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-16
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2   TEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCA 61
           +E +RRQL          ARR +++Y AL KNN+PC RRG SYYDCKKR+R NPYRRGC+
Sbjct: 50  SETNRRQL---------AARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCS 107

Query: 62  AITGCAR 69
            IT C R
Sbjct: 110 VITHCYR 107

[Close]

BLAST of Csor.00g260850 vs. TAIR 10
Match: AT1G28270.1 (ralf-like 4 )

HSP 1 Score: 80.1 bits (196), Expect = 8.7e-16
Identity = 40/67 (59.70%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2   TEDSRRQLFQYGFAYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCA 61
           +E +RRQL +          RR++ Y AL KNN+PC RRG SYYDCKKRRR NPYRRGC+
Sbjct: 50  SETNRRQLAR---------GRRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCS 107

Query: 62  AITGCAR 69
           AIT C R
Sbjct: 110 AITHCYR 107

[Close]

BLAST of Csor.00g260850 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 69.3 bits (168), Expect = 1.5e-12
Identity = 29/50 (58.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCARS 70
           A RR+++Y AL +N IPC RRG SYY+C++  + NPY RGC+AIT C RS
Sbjct: 89  ATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRRS 138

[Close]

BLAST of Csor.00g260850 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 63.5 bits (153), Expect = 8.4e-11
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCAR 69
           A  ++++Y AL +N +PC RRG SYY+C++  + NPY RGC+AIT C R
Sbjct: 68  ATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Csor.00g260850 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 63.2 bits (152), Expect = 1.1e-10
Identity = 23/49 (46.94%), Postives = 38/49 (77.55%), Query Frame = 0


Query: 20  ARRRFLTYYALSKNNIPCGRRGTSYYDCKKRRRINPYRRGCAAITGCAR 69
           A++++++Y A+ +N++PC RRG SYY+C++  + NPY RGC+ IT C R
Sbjct: 71  AQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCRR 119

The following BLAST results are available for this feature:

BLAST of Csor.00g260850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NME6	4.2e-15	58.21	Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1	[more]
Q9FZA0	1.2e-14	59.70	Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=1 SV=1	[more]
Q9LUS7	2.2e-11	58.00	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
Q945T0	3.1e-10	55.32	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q8L9P8	1.2e-09	51.02	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of Csor.00g260850 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588677.1	9.21e-51	100.00	Protein RALF-like 19, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022927734.1	6.06e-42	100.00	protein RALF-like 4 [Cucurbita moschata]	[more]
KAG6588678.1	6.25e-41	97.06	Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022465...	[more]
XP_022927735.1	7.06e-41	97.06	protein RALF-like 4 [Cucurbita moschata]	[more]
KAG6588679.1	2.54e-40	95.59	Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022466...	[more]

BLAST of Csor.00g260850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EIU0	2.93e-42	100.00	protein RALF-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111434547 PE=3 SV=1	[more]
A0A6J1EPT5	3.42e-41	97.06	protein RALF-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111434548 PE=3 SV=1	[more]
A0A6J1EIG3	1.35e-40	95.59	rapid alkalinization factor 23-like OS=Cucurbita moschata OX=3662 GN=LOC11143454...	[more]
A0A6J1JL71	2.72e-40	95.59	protein RALF-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111486206 PE=3 SV=1	[more]
A0A6J1JIT9	8.28e-40	94.12	protein RALF-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111486203 PE=3 SV=1	[more]

BLAST of Csor.00g260850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33775.1	3.0e-16	58.21	ralf-like 19	[more]
AT1G28270.1	8.7e-16	59.70	ralf-like 4	[more]
AT3G16570.1	1.5e-12	58.00	rapid alkalinization factor 23	[more]
AT4G15800.1	8.4e-11	51.02	ralf-like 33	[more]
AT3G05490.1	1.1e-10	46.94	ralf-like 22	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 20..68 e-value: 9.8E-19 score: 68.1
None	No IPR available	PANTHER	PTHR33136:SF79	BNAA07G08550D PROTEIN	coord: 21..71
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 21..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g260850.m01	Csor.00g260850.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region