Csor.00g255050 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g255050
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCsor_Chr06: 8814253 .. 8815494 (-)
RNA-Seq ExpressionCsor.00g255050
SyntenyCsor.00g255050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGATTTTCCTCCTCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATTACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTCCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATTGAAACGAGCGATGAGATGAAATCGGAATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAAGTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCGGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGCCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGTGGCGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATCGAATGA

mRNA sequence

ATGGATTCCGATTTTCCTCCTCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATTACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTCCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATTGAAACGAGCGATGAGATGAAATCGGAATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAAGTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCGGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGCCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGTGGCGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATCGAATGA

Coding sequence (CDS)

ATGGATTCCGATTTTCCTCCTCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCACTCTCAAACGCCTCATTCTCGCTTGGAAGATTACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTCCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATTGAAACGAGCGATGAGATGAAATCGGAATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAAGTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCGGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGCCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGTGGCGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATCGAATGA

Protein sequence

MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Homology
BLAST of Csor.00g255050 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.5e-55
Identity = 140/416 (33.65%), Postives = 221/416 (53.12%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TP
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTP 70

Query: 64  NLTLKRLILAWKITDSS---------ASPATIEEVRSLLA-TVESSPFKVSSLRKLRSMI 123
           N TL+RLI +W   ++S           P    E+  L+  +  S   +V  L++LR ++
Sbjct: 71  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 130

Query: 124 ETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDE 183
             +   K           + N+V       +D       +EA+ +L  L  S E  L + 
Sbjct: 131 SENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNL 190

Query: 184 MSKREE--LMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLE 243
           ++ +++  ++KS+  ++QRG  E R YA  +L+NI +  +      L+    + F  +++
Sbjct: 191 LNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQ 250

Query: 244 LVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPE----SGRSKCERM 303
           ++ D  S KA+  A+ +++ I     R+R KA+EAG + ++IELL +    S R   E  
Sbjct: 251 ILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEMA 310

Query: 304 LQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVE 363
           + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     ++ 
Sbjct: 311 MVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLH 370

Query: 364 EMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSEL 404
           EM+  GVV KL ++L +  GG         TKEKA ++LK+H   W+ SPC P  +
Sbjct: 371 EMLQLGVVAKLCLVLQVSCGG--------KTKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of Csor.00g255050 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.9e-50
Identity = 139/438 (31.74%), Postives = 221/438 (50.46%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TP
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTP 65

Query: 64  NLTLKRLILAWKITDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMI 123
           N TL+RLI +W   ++S           P    E+  L+    SS   +V  L++LR ++
Sbjct: 66  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 125

Query: 124 ETSDEMKSEFVRLNGIGIVVNVVVQIL-------IDCSDFWTFEAC-------------- 183
             +   K           + N+V   +          S     + C              
Sbjct: 126 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 185

Query: 184 --EEAVGVLSQLPISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKN 243
             +EA+ VL  L  S    K      K   L+K++  ++QRG  E R YA  +L+ + + 
Sbjct: 186 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 245

Query: 244 TNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAV 303
            +     +LE    + F  +++++ D+ S KA+  A+ +++ I     R+R KA+E G +
Sbjct: 246 ADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGGTI 305

Query: 304 CILIELLPE----SGRSKCERMLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVA 363
            ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS + 
Sbjct: 306 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 365

Query: 364 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKI 404
           ++  V++LL +  F     +++EM+  GVV KL ++L +  G        + TKEKA ++
Sbjct: 366 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG--------NKTKEKAKEL 425

BLAST of Csor.00g255050 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.6e-36
Identity = 131/459 (28.54%), Postives = 216/459 (47.06%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TP
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL-DSDLTP 68

Query: 64  NLTLKRLILAW----------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLR 123
           N  L+RLI  W          +I+     P     +EE+++L    + +  +  +L+KL 
Sbjct: 69  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 128

Query: 124 SMIETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDK 183
            +    +  +          +++ VV     D       +  +E++ +L  + I SN+ K
Sbjct: 129 VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAK 188

Query: 184 LFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPL 243
               + + + +M+S+  VL +     + Y + +LRN+   T +    ++E    + FK +
Sbjct: 189 TI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEIFKGI 248

Query: 244 LELVSDEFST-----------------------------------KASSCALDVMMEILA 303
           +  + D  ++                                   +A + AL +++E  +
Sbjct: 249 IGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSS 308

Query: 304 LSKRSRVKAIEAGAVCILIEL-LPESGRSK-CERMLQILQSLCECAEGRLPLVEHGMGIA 363
            S R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H  GIA
Sbjct: 309 WS-RNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIA 368

Query: 364 AVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGGG 412
            VTK++L VS  A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     
Sbjct: 369 VVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----- 428

BLAST of Csor.00g255050 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.8e-33
Identity = 125/407 (30.71%), Postives = 195/407 (47.91%), Query Frame = 0

Query: 6   PPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL 65
           P  F CPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN 
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNH 74

Query: 66  TLKRLILAWKITDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKL 125
           TL+RLI  W + + S           PA    VR+LL+       T  S   + ++LR+L
Sbjct: 75  TLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRL 134

Query: 126 RSMIETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDK 185
           R     SD+ +      N   I++ ++       S+  + E   E++ +L  LPI+ E  
Sbjct: 135 RGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPN 194

Query: 186 LFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFK 245
            F  +S     ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+
Sbjct: 195 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FE 254

Query: 246 PLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIE-LLPESGRSKCER 305
            +L+L+ +  S++ +       +  L   K +R  AI AGA  ILI+ L  +  R   ER
Sbjct: 255 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 314

Query: 306 MLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVV 365
            L  ++ LC   EG     EH + +  + K IL VS+ AT+     LL + +    ER  
Sbjct: 315 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEERWR 374

Query: 366 EEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSW 394
           EE    GVV +L++++  +             K+KA K+LK+   SW
Sbjct: 375 EEAAGAGVVVQLLLMVQSE--------CTERAKKKAQKLLKLLRDSW 400

BLAST of Csor.00g255050 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.4e-30
Identity = 119/417 (28.54%), Postives = 196/417 (47.00%), Query Frame = 0

Query: 6   PPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL 65
           PP F CPIS++LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN 
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNH 91

Query: 66  TLKRLILAWKITDSSASPATIEEVRSLLATVE--------SSPFKVSSLRKLRSMIETSD 125
           T++++I  W +   S     I   R  L   E        SS  +     K   +IE   
Sbjct: 92  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 151

Query: 126 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE-- 185
           ++  E  + N   +  N V  +L DC D   F   E+   +L+++        PI  E  
Sbjct: 152 KLGDESEK-NRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGI 211

Query: 186 DKLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFK 245
            KL    S      + +A +L+      R  A  I++ I         S   E G+   +
Sbjct: 212 SKLASATS-----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AE 271

Query: 246 PLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERM 305
            L++L+ D  S+ ++  +L  + +++        + +E G V I +E++ ++  S CE+ 
Sbjct: 272 ALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKA 331

Query: 306 LQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATK--MGVKILLWISSFHPNERV 365
           L +L ++CE   GR  + ++ + +  + KKI  VS +AT+  M + + LW +    N   
Sbjct: 332 LAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---NTVA 391

Query: 366 VEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWR-RSPCFPS 402
           VE+ +  G  +K++++L +  G          TKEKA ++LKM  +  +  S C  S
Sbjct: 392 VEDAVRLGAFQKVLLVLQVGYG--------EETKEKATELLKMMNTQMKLMSDCVDS 424

BLAST of Csor.00g255050 vs. NCBI nr
Match: KAG6597231.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806 bits (2083), Expect = 2.88e-294
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Csor.00g255050 vs. NCBI nr
Match: KAG7028702.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 803 bits (2075), Expect = 4.77e-293
Identity = 412/413 (99.76%), Postives = 412/413 (99.76%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Csor.00g255050 vs. NCBI nr
Match: XP_022959873.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 802 bits (2071), Expect = 1.94e-292
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSC LDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Csor.00g255050 vs. NCBI nr
Match: XP_022974967.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 783 bits (2022), Expect = 4.91e-285
Identity = 405/413 (98.06%), Postives = 405/413 (98.06%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNL LKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           S FVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSCALDVMMEILALSK SRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCALDVMMEILALSKSSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGG    RSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGG----RSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 409

BLAST of Csor.00g255050 vs. NCBI nr
Match: XP_022959792.1 (E3 ubiquitin-protein ligase PUB23-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 776 bits (2004), Expect = 3.41e-282
Identity = 398/400 (99.50%), Postives = 398/400 (99.50%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSC LDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 400
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 400

BLAST of Csor.00g255050 vs. ExPASy TrEMBL
Match: A0A6J1H765 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 802 bits (2071), Expect = 9.41e-293
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSC LDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

BLAST of Csor.00g255050 vs. ExPASy TrEMBL
Match: A0A6J1IKT9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 PE=4 SV=1)

HSP 1 Score: 783 bits (2022), Expect = 2.38e-285
Identity = 405/413 (98.06%), Postives = 405/413 (98.06%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNL LKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           S FVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSCALDVMMEILALSK SRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCALDVMMEILALSKSSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           ILHMDGGGGG    RSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE
Sbjct: 361 ILHMDGGGGG----RSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 409

BLAST of Csor.00g255050 vs. ExPASy TrEMBL
Match: A0A6J1H5I9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 1.65e-282
Identity = 398/400 (99.50%), Postives = 398/400 (99.50%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60
           MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFV 60

Query: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120
           ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK
Sbjct: 61  ITPNLTLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMK 120

Query: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180
           SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL
Sbjct: 121 SEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 180

Query: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240
           MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK
Sbjct: 181 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTK 240

Query: 241 ASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300
           ASSC LDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG
Sbjct: 241 ASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCECAEG 300

Query: 301 RLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360
           RL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM
Sbjct: 301 RLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMM 360

Query: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 400
           ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 361 ILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 400

BLAST of Csor.00g255050 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 697 bits (1798), Expect = 6.24e-251
Identity = 363/414 (87.68%), Postives = 383/414 (92.51%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYN +TCPATMQT+A G  D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLTLKRLILAWKITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120
           FV+TPNLTLKR+ILAWKI +SS+SPAT I++V+SLLATVESSPFKVSSLRKLRSMIE  D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180
           E+KSEF+RLNGIGIVVNVVVQILIDCSDF T EACEEA+GVLSQ PISNED+LFDEMSKR
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192

Query: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKS+AIVLQRGSAEGRFYAVKILRNIAKNTN+NWG V+EEQGID FKPLLELVSDEF
Sbjct: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 STKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300
            TK+SSCALDVM+EILA SKRSRVKAIEAGAV ILIELLPES RSKCERMLQILQSLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360
           AEGRL LVEHGMGIAAVTKKILNVSNVATK+GVKIL WISSFHP ERVVEEMMACG VKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVN 411
           L+MILHM    G GGGGRSSTKEK IKILKMHGSSWRRSPCFP ELKNYL  VN
Sbjct: 373 LLMILHM-ACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVN 425

BLAST of Csor.00g255050 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 8.06e-251
Identity = 360/416 (86.54%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--D 60
           MDSDFPPLF CPISMELMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTLAAG  D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLTLKRLILAWKITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120
           FVITPNL LKRLILAWKI DS+ +PAT +EEV SLLATVESSPFKVSSLRKLRSM+E +D
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180
            MKSEF+RL+GIGIVVNVV+QILIDCSDF T + CEEA+GVL Q PISNEDKLFDEM KR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EELMKSIA+V+QRGSAEGRFYAVKILRNIAKNTN+NWGS++EEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 241 STKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVM+EIL+ SKRSRVKAIEAGAV ILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360
           AEGRL LVEHGMGIAAVTKKILNVS VATK+GVKILLWISSFHP ERVV+EMMACG VKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413
           L+MILHMD  G G GG RSSTKEK IKILKMHG+ WRRSPCFPSELKNYL FVN E
Sbjct: 361 LLMILHMDSTGVGDGG-RSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of Csor.00g255050 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 216.1 bits (549), Expect = 5.4e-56
Identity = 140/416 (33.65%), Postives = 221/416 (53.12%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TP
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTP 70

Query: 64  NLTLKRLILAWKITDSS---------ASPATIEEVRSLLA-TVESSPFKVSSLRKLRSMI 123
           N TL+RLI +W   ++S           P    E+  L+  +  S   +V  L++LR ++
Sbjct: 71  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 130

Query: 124 ETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDE 183
             +   K           + N+V       +D       +EA+ +L  L  S E  L + 
Sbjct: 131 SENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNL 190

Query: 184 MSKREE--LMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLE 243
           ++ +++  ++KS+  ++QRG  E R YA  +L+NI +  +      L+    + F  +++
Sbjct: 191 LNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQ 250

Query: 244 LVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPE----SGRSKCERM 303
           ++ D  S KA+  A+ +++ I     R+R KA+EAG + ++IELL +    S R   E  
Sbjct: 251 ILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEMA 310

Query: 304 LQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVE 363
           + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     ++ 
Sbjct: 311 MVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLH 370

Query: 364 EMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSEL 404
           EM+  GVV KL ++L +  GG         TKEKA ++LK+H   W+ SPC P  +
Sbjct: 371 EMLQLGVVAKLCLVLQVSCGG--------KTKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of Csor.00g255050 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-51
Identity = 139/438 (31.74%), Postives = 221/438 (50.46%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TP
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTP 65

Query: 64  NLTLKRLILAWKITDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMI 123
           N TL+RLI +W   ++S           P    E+  L+    SS   +V  L++LR ++
Sbjct: 66  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 125

Query: 124 ETSDEMKSEFVRLNGIGIVVNVVVQIL-------IDCSDFWTFEAC-------------- 183
             +   K           + N+V   +          S     + C              
Sbjct: 126 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 185

Query: 184 --EEAVGVLSQLPISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKN 243
             +EA+ VL  L  S    K      K   L+K++  ++QRG  E R YA  +L+ + + 
Sbjct: 186 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 245

Query: 244 TNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAV 303
            +     +LE    + F  +++++ D+ S KA+  A+ +++ I     R+R KA+E G +
Sbjct: 246 ADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGGTI 305

Query: 304 CILIELLPE----SGRSKCERMLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVA 363
            ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS + 
Sbjct: 306 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 365

Query: 364 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKI 404
           ++  V++LL +  F     +++EM+  GVV KL ++L +  G        + TKEKA ++
Sbjct: 366 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG--------NKTKEKAKEL 425

BLAST of Csor.00g255050 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 153.7 bits (387), Expect = 3.3e-37
Identity = 131/459 (28.54%), Postives = 216/459 (47.06%), Query Frame = 0

Query: 4   DFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITP 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TP
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL-DSDLTP 82

Query: 64  NLTLKRLILAW----------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLR 123
           N  L+RLI  W          +I+     P     +EE+++L    + +  +  +L+KL 
Sbjct: 83  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 142

Query: 124 SMIETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDK 183
            +    +  +          +++ VV     D       +  +E++ +L  + I SN+ K
Sbjct: 143 VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAK 202

Query: 184 LFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPL 243
               + + + +M+S+  VL +     + Y + +LRN+   T +    ++E    + FK +
Sbjct: 203 TI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEIFKGI 262

Query: 244 LELVSDEFST-----------------------------------KASSCALDVMMEILA 303
           +  + D  ++                                   +A + AL +++E  +
Sbjct: 263 IGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSS 322

Query: 304 LSKRSRVKAIEAGAVCILIEL-LPESGRSK-CERMLQILQSLCECAEGRLPLVEHGMGIA 363
            S R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H  GIA
Sbjct: 323 WS-RNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIA 382

Query: 364 AVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGGG 412
            VTK++L VS  A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     
Sbjct: 383 VVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----- 442

BLAST of Csor.00g255050 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 143.7 bits (361), Expect = 3.4e-34
Identity = 125/407 (30.71%), Postives = 195/407 (47.91%), Query Frame = 0

Query: 6   PPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL 65
           P  F CPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN 
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNH 74

Query: 66  TLKRLILAWKITDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKL 125
           TL+RLI  W + + S           PA    VR+LL+       T  S   + ++LR+L
Sbjct: 75  TLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRL 134

Query: 126 RSMIETSDEMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDK 185
           R     SD+ +      N   I++ ++       S+  + E   E++ +L  LPI+ E  
Sbjct: 135 RGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPN 194

Query: 186 LFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFK 245
            F  +S     ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+
Sbjct: 195 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FE 254

Query: 246 PLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIE-LLPESGRSKCER 305
            +L+L+ +  S++ +       +  L   K +R  AI AGA  ILI+ L  +  R   ER
Sbjct: 255 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 314

Query: 306 MLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVV 365
            L  ++ LC   EG     EH + +  + K IL VS+ AT+     LL + +    ER  
Sbjct: 315 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEERWR 374

Query: 366 EEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSW 394
           EE    GVV +L++++  +             K+KA K+LK+   SW
Sbjct: 375 EEAAGAGVVVQLLLMVQSE--------CTERAKKKAQKLLKLLRDSW 400

BLAST of Csor.00g255050 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 4.6e-31
Identity = 119/417 (28.54%), Postives = 196/417 (47.00%), Query Frame = 0

Query: 6   PPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL 65
           PP F CPIS++LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN 
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNH 91

Query: 66  TLKRLILAWKITDSSASPATIEEVRSLLATVE--------SSPFKVSSLRKLRSMIETSD 125
           T++++I  W +   S     I   R  L   E        SS  +     K   +IE   
Sbjct: 92  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKIK 151

Query: 126 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE-- 185
           ++  E  + N   +  N V  +L DC D   F   E+   +L+++        PI  E  
Sbjct: 152 KLGDESEK-NRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGI 211

Query: 186 DKLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFK 245
            KL    S      + +A +L+      R  A  I++ I         S   E G+   +
Sbjct: 212 SKLASATS-----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AE 271

Query: 246 PLLELVSDEFSTKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERM 305
            L++L+ D  S+ ++  +L  + +++        + +E G V I +E++ ++  S CE+ 
Sbjct: 272 ALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKA 331

Query: 306 LQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATK--MGVKILLWISSFHPNERV 365
           L +L ++CE   GR  + ++ + +  + KKI  VS +AT+  M + + LW +    N   
Sbjct: 332 LAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---NTVA 391

Query: 366 VEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWR-RSPCFPS 402
           VE+ +  G  +K++++L +  G          TKEKA ++LKM  +  +  S C  S
Sbjct: 392 VEDAVRLGAFQKVLLVLQVGYG--------EETKEKATELLKMMNTQMKLMSDCVDS 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TG37.5e-5533.65E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC61.9e-5031.74E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF154.6e-3628.54E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT794.8e-3330.71U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q5PNY66.4e-3028.54U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6597231.12.88e-294100.00E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7028702.14.77e-29399.76E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022959873.11.94e-29299.52E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata][more]
XP_022974967.14.91e-28598.06E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubi... [more]
XP_022959792.13.41e-28299.50E3 ubiquitin-protein ligase PUB23-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1H7659.41e-29399.52RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728... [more]
A0A6J1IKT92.38e-28598.06RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 P... [more]
A0A6J1H5I91.65e-28299.50RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728... [more]
A0A6J1IYW36.24e-25187.68RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P... [more]
A0A0A0L6J28.06e-25186.54RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE... [more]
Match NameE-valueIdentityDescription
AT2G35930.15.4e-5633.65plant U-box 23 [more]
AT3G52450.11.4e-5131.74plant U-box 22 [more]
AT3G11840.13.3e-3728.54plant U-box 24 [more]
AT3G19380.13.4e-3430.71plant U-box 25 [more]
AT5G37490.14.6e-3128.54ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 8..74
e-value: 2.2E-21
score: 87.0
IPR003613U box domainPFAMPF04564U-boxcoord: 6..75
e-value: 1.2E-14
score: 54.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 4..81
score: 22.257547
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 4..72
e-value: 3.0E-21
score: 77.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 73..388
e-value: 2.1E-15
score: 58.2
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 5..411
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 5..411
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 9..52
e-value: 4.27763E-20
score: 80.7407
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..77
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 85..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g255050.m01Csor.00g255050.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity