Csor.00g251900 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g251900 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 568.9 bits (1465), Expect = 6.3e-161
Identity = 280/574 (48.78%), Postives = 389/574 (67.77%), Query Frame = 0

Query: 3   YDGGGCKKNKAALVGLLSLFFVVLV------------NGVSGAGEEPGSSNAAIKTLCEP 62
           YD    +K +  ++ + S+  + +V            +G S    E  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F+ H ++  L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEP 242
            GE MK  LKT+ E+T NGL ++ E+++ V     ++ +     R+L         EG P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRL-------LAEGFP 244

Query: 243 SWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGV 302
           SWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EAL+ VP K NTTFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 303 YEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFEN 362
           Y+E V V+K M++++ IGDGP KT I+G K+ +DG  T+++AT A VG+ FI K++ FEN
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 363 SAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAV 422
           +AG  K QAVA+RVQSD SIF+NCR DGYQDT Y H+ RQF+RDCTISGT+DF+FG+AA 
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 423 VLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGR 482
           V QNC +LVRKP P Q CPITA GR +  E TG V Q CTI+ EPDY  V+ TSK+YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 483 PWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVK 542
           PWK+YSRT+IM   I D +QP+GW PW GDF L TL+Y+E  N GPG+A  NRV W G+K
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 543 QITAKQAINFTPSLFIQGDDWIKNTGIPYTSALM 562
            ++ +  + FTP+ +IQGDDWI   G+PYT+ L+
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g251900 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.2e-157
Identity = 281/570 (49.30%), Postives = 380/570 (66.67%), Query Frame = 0

Query: 3   YDGGGCKKNKA-ALVGLLSLFFV---------VLVNGVSGAG-EEPGSSNAAIKTLCEPT 62
           YD    K+ K   ++ + S+  +         V VN    AG EE  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F+ H+V+  L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPS 242
           GE +K  LKT+ ++T NGL MV E+++ +     ++ +     R+L ++E        PS
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQEF-------PS 244

Query: 243 WVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEE 302
           W+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP K NTTFV+++KEG+Y+E
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 304

Query: 303 QVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAG 362
            V V++ MT+++ IGDGP KT I+G KS +DG  T+K+AT A VG +FI K++ FEN+AG
Sbjct: 305 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 364

Query: 363 LYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQ 422
             K QAVA+RV +D SIFYNC+ DGYQDT Y H+ RQFYRDCTISGT+DF+FG+AA V Q
Sbjct: 365 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 424

Query: 423 NCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWK 482
           NC +LVRKP   Q CPITA GR +  E TG VLQ CTI  EPDY  V+  SK+YLGRPWK
Sbjct: 425 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 484

Query: 483 QYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT 542
           +YSRT+IM   I D + PEGW PW G+F LNTL+Y+E  N GPGAA   RV W G+K+++
Sbjct: 485 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 544

Query: 543 AKQAINFTPSLFIQGDDWIKNTGIPYTSAL 561
            ++ + FTP+ +IQGD WI   G+PY   L
Sbjct: 545 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Csor.00g251900 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.9e-150
Identity = 269/566 (47.53%), Postives = 370/566 (65.37%), Query Frame = 0

Query: 6   GGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGS------------SNAAIKTLCEPTTY 65
           G  KK K  + G+++   V++V  V        S            +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDA 125
           +ETC   L+K++ DS  P +L+K GF+  I  ++ +I  A+     +AA D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   D F + ++  ++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPS 245
            + M+ + KTSRE+T+NGL M+  +++L+   +   G+    G+  R   S E  +G PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKYARKLLSAE--DGIPS 244

Query: 246 WVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQ 305
           WV      L AT   +KANVVVA DGSG+YKTI EAL  VP  +   FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 306 VLVDKKMTYVMVIGDGPTKTKITGRKSVEDG-TPTFKSATFAAVGSNFIGKDLWFENSAG 365
           V V KKMT+V  IGDGPTKTKITG  +   G   T+ +AT A  G NF  K++ FEN+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 366 LYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQ 425
               QAVALRV +D+++FYNC++DGYQDT Y H+ RQF+RDCT+SGTVDFIFG+  VVLQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 426 NCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWK 485
           NC I+VRKP   Q C ITAQGR +  E TG+VLQNC I+ EP Y PV+  +K+YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 486 QYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT 545
           ++SRT+IM   IDD+I P GWLPWNGDFALNTLYY E++N GPG+ +  RVKW G+K+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 546 AKQAINFTPSLFIQGDDWIKNTGIPY 557
            KQA+ FTP+ F++G+ WI    +PY
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g251900 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-136
Identity = 248/570 (43.51%), Postives = 365/570 (64.04%), Query Frame = 0

Query: 7   GCKKNKAALVGLLSLFFVVLVNGVSGAGEEPG---------SSNAAIKTLCEPTTYRETC 66
           G KK K  + G +S F V++V  V+    +            +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDACDEL 126
              L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D     F V ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKTSREMTANGLVMVGEVTSLV--STLWEKLGLLPQTGRQLRTEESNEYQEGEPSWV 246
             + KTSRE+++N L MV  +++L+  S L    G L +  R+L + E     +  P+WV
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE-----DSIPTWV 244

Query: 247 S-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYE 306
             + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +   F+I++KEG+Y+
Sbjct: 245 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYK 304

Query: 307 EQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDG-TPTFKSATFAAVGSNFIGKDLWFENS 366
           E+V V KKM +V  IGDGP KT ITG  +   G   TF +AT    G +F  K++  EN+
Sbjct: 305 EKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENT 364

Query: 367 AGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVV 426
           AG   GQAVALRV +D ++F++C++DG+QDT Y H+ RQFYRDCT+SGTVDFIFG+A  +
Sbjct: 365 AGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCI 424

Query: 427 LQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRP 486
           LQNCKI+VRKP   Q C +TAQGR    E TG+VL  C I+ +P Y P++  +K+YLGRP
Sbjct: 425 LQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRP 484

Query: 487 WKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQ 546
           WK++SRT+IM+  IDD+I P GWLPW+GDFAL TLYY E  N GPG+ +  RVKW G+K+
Sbjct: 485 WKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKK 544

Query: 547 ITAKQAINFTPSLFIQGDDWIKNTGIPYTS 559
           +T + A+ +T   F++GD WI  T +PYT+
Sbjct: 545 LTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Csor.00g251900 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 8.0e-116
Identity = 248/702 (35.33%), Postives = 364/702 (51.85%), Query Frame = 0

Query: 11  NKAALVGLLSLFFVVLVNGVSGAGEEPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L   +++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQAATDPMAQKAVDACDEL 130
              L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  + DK    F   R++ ++E+L +WLS ++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSREMTANGLVMVGEVTSLVSTL----------WEKLGLL--------------- 250
           + +++  + +T+NGL +   +  L+               +LG+L               
Sbjct: 182 ERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQD 241

Query: 251 -----------PQT------------------------------GRQLRTEE-------- 310
                      P T                               +Q+ + E        
Sbjct: 242 SPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAF 301

Query: 311 ------------------SNEYQ------------------------------------- 370
                             S+E Q                                     
Sbjct: 302 SGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKD 361

Query: 371 -----EGE-PSWVSDR-RGLLEATGAN--IKANVVVAKDGSGKYKTITEALKEVPLKSNT 430
                EGE P WV+   R LL     N  IKANVVVAKDGSGK KTI +AL  VP+K+  
Sbjct: 362 RHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTK 421

Query: 431 TFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKS-VEDGTPTFKSATFAAVGS 490
            FVI++KEGVY+E+V V KKM +VM +GDGPTKT ITG  + + D   T+++A+ A  G 
Sbjct: 422 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 481

Query: 491 NFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISG 550
            F+ KD+ FEN+AG  + QAVALRV +D ++F+NC M+GYQDT Y H  RQFYR+C +SG
Sbjct: 482 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 541

Query: 551 TVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYP 559
           T+DF+FG+A  V QNC+ ++R+P   Q C +TAQGR +  E TGIV+ N  I+ +  Y P
Sbjct: 542 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 601

BLAST of Csor.00g251900 vs. NCBI nr
Match: KAG6597545.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1142 bits (2954), Expect = 0.0
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
           TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL
Sbjct: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR
Sbjct: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK
Sbjct: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC
Sbjct: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS
Sbjct: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFIQGDDWIKNTGIPYTSALMKI
Sbjct: 541 LFIQGDDWIKNTGIPYTSALMKI 563

BLAST of Csor.00g251900 vs. NCBI nr
Match: XP_023539372.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1092 bits (2824), Expect = 0.0
Identity = 537/564 (95.21%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLF V LVNGVSGAGE+PGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGAGEDPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLK AIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKVAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVN YLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNE-YQEGEPSWVSDRRGL 240
           TS+EMTANGLVMVGEVTSLVSTLWEKLGL PQTGRQLRTEESNE +QE EPSWVSDRRGL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEEFQEEEPSWVSDRRGL 240

Query: 241 LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMT 300
           LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVI+VKEGVYEEQV+VDKKMT
Sbjct: 241 LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIHVKEGVYEEQVMVDKKMT 300

Query: 301 YVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVAL 360
           YVM+IGDGPTKTKIT  K+V DGTPTFKSATFAA+GSNFIGKDLWF+NSAG+YKGQAVAL
Sbjct: 301 YVMMIGDGPTKTKITASKNVADGTPTFKSATFAAIGSNFIGKDLWFDNSAGIYKGQAVAL 360

Query: 361 RVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 420
           RVQSDMSIFYNCRMDGYQDTFYP  KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP
Sbjct: 361 RVQSDMSIFYNCRMDGYQDTFYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 420

Query: 421 KPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQ 480
           KPKQPCPITAQGRMESDEPTGIVLQNC ISS+PDYYPVRHTSKSYLGRPWKQYSRTVIMQ
Sbjct: 421 KPKQPCPITAQGRMESDEPTGIVLQNCIISSDPDYYPVRHTSKSYLGRPWKQYSRTVIMQ 480

Query: 481 CQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTP 540
           CQIDDLIQPEGWLPWNGDFALNTLYYTEF+NRGPGAAKENRVKWKGVKQITAKQAINFTP
Sbjct: 481 CQIDDLIQPEGWLPWNGDFALNTLYYTEFENRGPGAAKENRVKWKGVKQITAKQAINFTP 540

Query: 541 SLFIQGDDWIKNTGIPYTSALMKI 563
           SLFIQGDDWIK TGIPYTSALMKI
Sbjct: 541 SLFIQGDDWIKKTGIPYTSALMKI 564

BLAST of Csor.00g251900 vs. NCBI nr
Match: KAG6597544.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 535/563 (95.03%), Postives = 547/563 (97.16%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLFFV LVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
           TS+EMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVY+EQV+VDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYKEQVMVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VM+IGDGPTKTKIT  K+V DGTPTFKSATFAA+GSNFIGKDLWF+NSAG  K QAVALR
Sbjct: 301 VMMIGDGPTKTKITANKNVIDGTPTFKSATFAAIGSNFIGKDLWFDNSAGPKKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMD YQDT YP  KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 
Sbjct: 361 VQSDMSIFYNCRMDAYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPL 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           PKQPCPITAQGRMESDEPTGIVLQNC IS++P++YP+RHTSKSYLGRPWKQYSRTVIMQC
Sbjct: 421 PKQPCPITAQGRMESDEPTGIVLQNCIISADPEFYPIRHTSKSYLGRPWKQYSRTVIMQC 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT KQAINFTPS
Sbjct: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITTKQAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFIQGDDWIKN GIPYTSALMKI
Sbjct: 541 LFIQGDDWIKNIGIPYTSALMKI 563

BLAST of Csor.00g251900 vs. NCBI nr
Match: XP_023539368.1 (pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539373.1 pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539374.1 pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539376.1 pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 535/564 (94.86%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLF V LVNGVSGAGE+ GSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGAGEDHGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVH++N YLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNE-YQEGEPSWVSDRRGL 240
           TS+EMTANGLVMVGEVTSLVSTLWEKLGL PQTGRQLRTEESNE +QE EPSWVSDRRGL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEEFQEEEPSWVSDRRGL 240

Query: 241 LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMT 300
           LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVI+VKEGVYEEQV+VDKKMT
Sbjct: 241 LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIHVKEGVYEEQVMVDKKMT 300

Query: 301 YVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVAL 360
           YVM+IGDGPTKTKIT  K+V DGTPTFKSATFAA+GSNFIGKDLWF+NSAG+YKGQAVAL
Sbjct: 301 YVMMIGDGPTKTKITASKNVADGTPTFKSATFAAIGSNFIGKDLWFDNSAGIYKGQAVAL 360

Query: 361 RVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 420
           RVQSDMSIFYNCRMDGYQDTFYP  KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP
Sbjct: 361 RVQSDMSIFYNCRMDGYQDTFYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 420

Query: 421 KPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQ 480
           KPKQPCPITAQGRMESDEPTGIVLQNC ISS+PDYYPVRHTSKSYLGRPWKQYSRTVIMQ
Sbjct: 421 KPKQPCPITAQGRMESDEPTGIVLQNCIISSDPDYYPVRHTSKSYLGRPWKQYSRTVIMQ 480

Query: 481 CQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTP 540
           CQIDDLIQPEGWLPWNGDFALNTLYYTEF+NRGPGAAKENRVKWKGVKQITAKQAINFTP
Sbjct: 481 CQIDDLIQPEGWLPWNGDFALNTLYYTEFENRGPGAAKENRVKWKGVKQITAKQAINFTP 540

Query: 541 SLFIQGDDWIKNTGIPYTSALMKI 563
           SLFIQGDDWIK TGIPYTSALMKI
Sbjct: 541 SLFIQGDDWIKKTGIPYTSALMKI 564

BLAST of Csor.00g251900 vs. NCBI nr
Match: KAG6597546.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1086 bits (2809), Expect = 0.0
Identity = 535/563 (95.03%), Postives = 547/563 (97.16%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
           TS+EMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVY+EQV+VDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYKEQVMVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VM+IGDGPTKTKIT  K+V DGTPTFKSATFAA+GSNFIGKDLWF+NSAG  K QAVALR
Sbjct: 301 VMMIGDGPTKTKITANKNVIDGTPTFKSATFAAIGSNFIGKDLWFDNSAGPKKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMD YQDT YP  KRQFYRDCTISGTVDFIFGNAAV LQNCKILVRKP 
Sbjct: 361 VQSDMSIFYNCRMDAYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAV-LQNCKILVRKPL 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           PKQPCPITAQGRMESDEPTGIVLQNC IS++P++YP+RHTSKSYLGRPWKQYSRTVIMQC
Sbjct: 421 PKQPCPITAQGRMESDEPTGIVLQNCIISADPEFYPIRHTSKSYLGRPWKQYSRTVIMQC 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT KQAINFTPS
Sbjct: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITTKQAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFIQGDDWIKN GIPYTSALMKI
Sbjct: 541 LFIQGDDWIKNIGIPYTSALMKI 562

BLAST of Csor.00g251900 vs. ExPASy TrEMBL
Match: A0A6J1IFP3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472354 PE=3 SV=1)

HSP 1 Score: 1060 bits (2741), Expect = 0.0
Identity = 524/563 (93.07%), Postives = 542/563 (96.27%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYD GGCK NKAALVGLLSLF V LVNGV+GAGE+ GSSNA IKTLCEPTTYRETCE+V
Sbjct: 1   MAYDRGGCKDNKAALVGLLSLFLVALVNGVNGAGEDHGSSNADIKTLCEPTTYRETCEKV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTL+QAATDP+AQ AVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLRQAATDPLAQNAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSF+KITDSFDVHRVN YLENLRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
           TS+EMTANGLVMVGEVTSLV+TLW+KLGLL QTGRQLRTE SNE +E EPSWVSDRRGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVTTLWQKLGLL-QTGRQLRTEHSNEEKE-EPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQV+VDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VMVIGDGPTKTKITG K+V DGT TFKSATFAAVGSNFIGKDLWF+NSAGLYKGQAVALR
Sbjct: 301 VMVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMDGYQDTFYPHA RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK
Sbjct: 361 VQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           PKQPCPITAQGRMESDEPTGIVLQNCTISS+PDYYPVRHTSKSYLGRPWKQYSRTV+MQ 
Sbjct: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQS 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKG+KQITAK+AINFTPS
Sbjct: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFI GD WIKNTGI YT ALMKI
Sbjct: 541 LFIHGDSWIKNTGISYTGALMKI 561

BLAST of Csor.00g251900 vs. ExPASy TrEMBL
Match: A0A6J1GBF1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452639 PE=3 SV=1)

HSP 1 Score: 1047 bits (2708), Expect = 0.0
Identity = 513/563 (91.12%), Postives = 535/563 (95.03%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLFFV LVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
           TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQ+GRQLRTEESNEYQ GEPSWVSDRRGLL
Sbjct: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQSGRQLRTEESNEYQAGEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VMVIGDGPTKTKIT  K+V DGTPTFKSATFAA+GSNFIGKDLWF+NSAG  K QAVALR
Sbjct: 301 VMVIGDGPTKTKITASKNVIDGTPTFKSATFAAIGSNFIGKDLWFDNSAGPKKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMD YQDT YP  KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 
Sbjct: 361 VQSDMSIFYNCRMDAYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPM 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           P Q C +TAQGR+E DEPTGIVLQNC IS++P++YP+RHTSK +LGRPWK+YSRTVIMQC
Sbjct: 421 PNQTCFVTAQGRVEIDEPTGIVLQNCIISADPEFYPIRHTSKIFLGRPWKKYSRTVIMQC 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           Q+DDLI PEGW+ W+ D  L TL+YTEFDNRGPGAAKENRVKW+G+KQITAKQAINFTPS
Sbjct: 481 QLDDLIHPEGWMRWSDDSTLKTLFYTEFDNRGPGAAKENRVKWEGIKQITAKQAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFIQGDDWIK++GIPYT  +MKI
Sbjct: 541 LFIQGDDWIKHSGIPYTGGMMKI 563

BLAST of Csor.00g251900 vs. ExPASy TrEMBL
Match: A0A6J1GCP0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452765 PE=3 SV=1)

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 508/563 (90.23%), Postives = 534/563 (94.85%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLF V LVNGVSG GE+ GSSNAAIKTLCEPTTYRETCEE 
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGGGEDSGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQA TDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQATTDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKIT+SF++H++N YLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITNSFNLHKMNDYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLL 240
            S+EMTANGLVMVGEVTSLVSTLWEKLGL PQTGRQLRTEESNEYQE EPSWVSDRRGLL
Sbjct: 181 MSKEMTANGLVMVGEVTSLVSTLWEKLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLL 240

Query: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTY 300
           EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQV+VDKKMTY
Sbjct: 241 EATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTY 300

Query: 301 VMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALR 360
           VM+IGDGPTKTKIT  K+V+DGTPTFKSATFAA+GSNFIGKDLWF+NSAGL+KGQAVALR
Sbjct: 301 VMMIGDGPTKTKITANKNVQDGTPTFKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPK 420
           VQSDMSIFYNCRMDGYQDT YP  KRQFYRDCTISGTVDFIFGNAA VLQNCKILVRKPK
Sbjct: 361 VQSDMSIFYNCRMDGYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPK 420

Query: 421 PKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQC 480
           P Q C +TAQGRMESDEPTGIVLQNC ISS+P++YP+RHTSKSYLGRPWK+YSRTVIMQC
Sbjct: 421 PNQACFVTAQGRMESDEPTGIVLQNCVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQC 480

Query: 481 QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPS 540
           QIDDLI P+GW+ W+ D  L TLYYTEFDNRGPGAAKENRVKWKG+KQITAKQAINFTPS
Sbjct: 481 QIDDLIHPDGWMRWSDDSTLKTLYYTEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPS 540

Query: 541 LFIQGDDWIKNTGIPYTSALMKI 563
           LFIQGDDWIKN+GIPYT  +MKI
Sbjct: 541 LFIQGDDWIKNSGIPYTGGMMKI 563

BLAST of Csor.00g251900 vs. ExPASy TrEMBL
Match: A0A6J1GBW0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452750 PE=3 SV=1)

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 537/652 (82.36%), Postives = 547/652 (83.90%), Query Frame = 0

Query: 1   MAYDGGGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60
           MAYDGGGCK NKAALVGLLSLFFV LVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSREMTANGL-------------------------------------------------- 240
           TS+EMTANGL                                                  
Sbjct: 181 TSKEMTANGLSSLCKTRIIIWTKSLQHVGDEFFILYSLANGSKLESSIRICIALARDVEA 240

Query: 241 ------------VMVGEVTSLVSTLWEKLGLLPQT---GRQ------------------- 300
                       V++  +    S++ ++L  L  +   G Q                   
Sbjct: 241 NCRSSVIQASRAVVIPTIVLRTSSVTDELMRLSTSWSWGYQTVNSGLMIILRFPVSSLGS 300

Query: 301 -----LRTEESNEYQEGEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEV 360
                L  EESNEYQEGEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEV
Sbjct: 301 TAIVWLGAEESNEYQEGEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEV 360

Query: 361 PLKSNTTFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATF 420
           PLKSNTTFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATF
Sbjct: 361 PLKSNTTFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATF 420

Query: 421 AAVGSNFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRD 480
           AAVGSNFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRD
Sbjct: 421 AAVGSNFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRD 480

Query: 481 CTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSE 540
           CTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISS+
Sbjct: 481 CTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSD 540

Query: 541 PDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNR 563
           PDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNR
Sbjct: 541 PDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNR 600

BLAST of Csor.00g251900 vs. ExPASy TrEMBL
Match: A0A6J1I9A9 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472265 PE=3 SV=1)

HSP 1 Score: 1039 bits (2687), Expect = 0.0
Identity = 517/562 (91.99%), Postives = 535/562 (95.20%), Query Frame = 0

Query: 6   GGCK----KNKAALVGLLSLFFVVLVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEVL 65
           GGC        AALVGLLSLF V LVNGV+GAGE+ GSSNA IKTLCEPTTYRETCE+VL
Sbjct: 13  GGCYIHPLSGTAALVGLLSLFLVALVNGVNGAGEDHGSSNADIKTLCEPTTYRETCEKVL 72

Query: 66  VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAID 125
           VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTL+QAATDP+AQ AVDACDELMDYAID
Sbjct: 73  VKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLRQAATDPLAQNAVDACDELMDYAID 132

Query: 126 DLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKT 185
           DLLTSF+KITDSFDVHRVN YLENLRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLKT
Sbjct: 133 DLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKT 192

Query: 186 SREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLLE 245
           S+EMTANGLVMVGEVTSLV+TLW+KLGLL QTGRQLRTE SNE +E EPSWVSDRRGLLE
Sbjct: 193 SKEMTANGLVMVGEVTSLVTTLWQKLGLL-QTGRQLRTEHSNEEKE-EPSWVSDRRGLLE 252

Query: 246 ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTYV 305
           ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQV+VDKKMTYV
Sbjct: 253 ATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYV 312

Query: 306 MVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALRV 365
           MVIGDGPTKTKITG K+V DGT TFKSATFAAVGSNFIGKDLWF+NSAGLYKGQAVALRV
Sbjct: 313 MVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRV 372

Query: 366 QSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKP 425
           QSDMSIFYNCRMDGYQDTFYPHA RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKP
Sbjct: 373 QSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKP 432

Query: 426 KQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQCQ 485
           KQPCPITAQGRMESDEPTGIVLQNCTISS+PDYYPVRHTSKSYLGRPWKQYSRTV+MQ Q
Sbjct: 433 KQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQ 492

Query: 486 IDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPSL 545
           IDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKG+KQITAK+AINFTPSL
Sbjct: 493 IDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSL 552

Query: 546 FIQGDDWIKNTGIPYTSALMKI 563
           FI GD WIKNTGI YT ALMKI
Sbjct: 553 FIHGDSWIKNTGISYTGALMKI 572

BLAST of Csor.00g251900 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 568.9 bits (1465), Expect = 4.4e-162
Identity = 280/574 (48.78%), Postives = 389/574 (67.77%), Query Frame = 0

Query: 3   YDGGGCKKNKAALVGLLSLFFVVLV------------NGVSGAGEEPGSSNAAIKTLCEP 62
           YD    +K +  ++ + S+  + +V            +G S    E  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F+ H ++  L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEP 242
            GE MK  LKT+ E+T NGL ++ E+++ V     ++ +     R+L         EG P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRL-------LAEGFP 244

Query: 243 SWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGV 302
           SWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EAL+ VP K NTTFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 303 YEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFEN 362
           Y+E V V+K M++++ IGDGP KT I+G K+ +DG  T+++AT A VG+ FI K++ FEN
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 363 SAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAV 422
           +AG  K QAVA+RVQSD SIF+NCR DGYQDT Y H+ RQF+RDCTISGT+DF+FG+AA 
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 423 VLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGR 482
           V QNC +LVRKP P Q CPITA GR +  E TG V Q CTI+ EPDY  V+ TSK+YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 483 PWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVK 542
           PWK+YSRT+IM   I D +QP+GW PW GDF L TL+Y+E  N GPG+A  NRV W G+K
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 543 QITAKQAINFTPSLFIQGDDWIKNTGIPYTSALM 562
            ++ +  + FTP+ +IQGDDWI   G+PYT+ L+
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g251900 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 556.6 bits (1433), Expect = 2.3e-158
Identity = 281/570 (49.30%), Postives = 380/570 (66.67%), Query Frame = 0

Query: 3   YDGGGCKKNKA-ALVGLLSLFFV---------VLVNGVSGAG-EEPGSSNAAIKTLCEPT 62
           YD    K+ K   ++ + S+  +         V VN    AG EE  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F+ H+V+  L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPS 242
           GE +K  LKT+ ++T NGL MV E+++ +     ++ +     R+L ++E        PS
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLLSQEF-------PS 244

Query: 243 WVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEE 302
           W+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP K NTTFV+++KEG+Y+E
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKE 304

Query: 303 QVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPTFKSATFAAVGSNFIGKDLWFENSAG 362
            V V++ MT+++ IGDGP KT I+G KS +DG  T+K+AT A VG +FI K++ FEN+AG
Sbjct: 305 YVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAG 364

Query: 363 LYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQ 422
             K QAVA+RV +D SIFYNC+ DGYQDT Y H+ RQFYRDCTISGT+DF+FG+AA V Q
Sbjct: 365 AIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQ 424

Query: 423 NCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWK 482
           NC +LVRKP   Q CPITA GR +  E TG VLQ CTI  EPDY  V+  SK+YLGRPWK
Sbjct: 425 NCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWK 484

Query: 483 QYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT 542
           +YSRT+IM   I D + PEGW PW G+F LNTL+Y+E  N GPGAA   RV W G+K+++
Sbjct: 485 EYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLS 544

Query: 543 AKQAINFTPSLFIQGDDWIKNTGIPYTSAL 561
            ++ + FTP+ +IQGD WI   G+PY   L
Sbjct: 545 DEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Csor.00g251900 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 533.5 bits (1373), Expect = 2.1e-151
Identity = 269/566 (47.53%), Postives = 370/566 (65.37%), Query Frame = 0

Query: 6   GGCKKNKAALVGLLSLFFVVLVNGVSGAGEEPGS------------SNAAIKTLCEPTTY 65
           G  KK K  + G+++   V++V  V        S            +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDA 125
           +ETC   L+K++ DS  P +L+K GF+  I  ++ +I  A+     +AA D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   D F + ++  ++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESNEYQEGEPS 245
            + M+ + KTSRE+T+NGL M+  +++L+   +   G+    G+  R   S E  +G PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKYARKLLSAE--DGIPS 244

Query: 246 WVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQ 305
           WV      L AT   +KANVVVA DGSG+YKTI EAL  VP  +   FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 306 VLVDKKMTYVMVIGDGPTKTKITGRKSVEDG-TPTFKSATFAAVGSNFIGKDLWFENSAG 365
           V V KKMT+V  IGDGPTKTKITG  +   G   T+ +AT A  G NF  K++ FEN+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 366 LYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVVLQ 425
               QAVALRV +D+++FYNC++DGYQDT Y H+ RQF+RDCT+SGTVDFIFG+  VVLQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 426 NCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRPWK 485
           NC I+VRKP   Q C ITAQGR +  E TG+VLQNC I+ EP Y PV+  +K+YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 486 QYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQIT 545
           ++SRT+IM   IDD+I P GWLPWNGDFALNTLYY E++N GPG+ +  RVKW G+K+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 546 AKQAINFTPSLFIQGDDWIKNTGIPY 557
            KQA+ FTP+ F++G+ WI    +PY
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g251900 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-137
Identity = 248/570 (43.51%), Postives = 365/570 (64.04%), Query Frame = 0

Query: 7   GCKKNKAALVGLLSLFFVVLVNGVSGAGEEPG---------SSNAAIKTLCEPTTYRETC 66
           G KK K  + G +S F V++V  V+    +            +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDACDEL 126
              L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D     F V ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKTSREMTANGLVMVGEVTSLV--STLWEKLGLLPQTGRQLRTEESNEYQEGEPSWV 246
             + KTSRE+++N L MV  +++L+  S L    G L +  R+L + E     +  P+WV
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE-----DSIPTWV 244

Query: 247 S-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYE 306
             + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +   F+I++KEG+Y+
Sbjct: 245 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYK 304

Query: 307 EQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDG-TPTFKSATFAAVGSNFIGKDLWFENS 366
           E+V V KKM +V  IGDGP KT ITG  +   G   TF +AT    G +F  K++  EN+
Sbjct: 305 EKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENT 364

Query: 367 AGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFGNAAVV 426
           AG   GQAVALRV +D ++F++C++DG+QDT Y H+ RQFYRDCT+SGTVDFIFG+A  +
Sbjct: 365 AGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCI 424

Query: 427 LQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYPVRHTSKSYLGRP 486
           LQNCKI+VRKP   Q C +TAQGR    E TG+VL  C I+ +P Y P++  +K+YLGRP
Sbjct: 425 LQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRP 484

Query: 487 WKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGVKQ 546
           WK++SRT+IM+  IDD+I P GWLPW+GDFAL TLYY E  N GPG+ +  RVKW G+K+
Sbjct: 485 WKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKK 544

Query: 547 ITAKQAINFTPSLFIQGDDWIKNTGIPYTS 559
           +T + A+ +T   F++GD WI  T +PYT+
Sbjct: 545 LTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Csor.00g251900 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 419.1 bits (1076), Expect = 5.7e-117
Identity = 248/702 (35.33%), Postives = 364/702 (51.85%), Query Frame = 0

Query: 11  NKAALVGLLSLFFVVLVNGVSGAGEEPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L   +++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQAATDPMAQKAVDACDEL 130
              L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  + DK    F   R++ ++E+L +WLS ++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSREMTANGLVMVGEVTSLVSTL----------WEKLGLL--------------- 250
           + +++  + +T+NGL +   +  L+               +LG+L               
Sbjct: 182 ERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQD 241

Query: 251 -----------PQT------------------------------GRQLRTEE-------- 310
                      P T                               +Q+ + E        
Sbjct: 242 SPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAF 301

Query: 311 ------------------SNEYQ------------------------------------- 370
                             S+E Q                                     
Sbjct: 302 SGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKD 361

Query: 371 -----EGE-PSWVSDR-RGLLEATGAN--IKANVVVAKDGSGKYKTITEALKEVPLKSNT 430
                EGE P WV+   R LL     N  IKANVVVAKDGSGK KTI +AL  VP+K+  
Sbjct: 362 RHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTK 421

Query: 431 TFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKS-VEDGTPTFKSATFAAVGS 490
            FVI++KEGVY+E+V V KKM +VM +GDGPTKT ITG  + + D   T+++A+ A  G 
Sbjct: 422 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 481

Query: 491 NFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISG 550
            F+ KD+ FEN+AG  + QAVALRV +D ++F+NC M+GYQDT Y H  RQFYR+C +SG
Sbjct: 482 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 541

Query: 551 TVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSEPDYYP 559
           T+DF+FG+A  V QNC+ ++R+P   Q C +TAQGR +  E TGIV+ N  I+ +  Y P
Sbjct: 542 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 601