Csor.00g251850 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g251850
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	exopolygalacturonase-like
Location	Csor_Chr06: 10503716 .. 10505318 (+)
RNA-Seq Expression	Csor.00g251850
Synteny	Csor.00g251850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTCTTCTTCGAAACTACCCGTCCTTCTCGGGTTGTTCTTAGCATCATTCGTTGCTGCCAAGGCAAAGGCGCTCATTGGACCGAGCACGCCCGACCCCTTTTCATCAATTAATCTAAACAGTATTACACATCGAAAGGAAGCCGTGTTTAACGTGCTTAGTTTCGGTGCCAAACTCGGTGGCACTAAAGACAACACTCAAGTAATTGTTAAGCATGTTTTGTTACCATCGTTGTCGTTGTCATCGTTGTCGTTTAATGATGTCTTTTTTGGTAGACTTTCATGAAGACATGGGTTGCCGCTTGTCGCTCCAAGCAACCGGCTAGAGTTGTTATTCCCAGTGGTGTGTTTTTGATCAATCAAATCGTCTTCGCTGGGCCTTGTCGGAACACTGAACCAATTGTCGTTCAAATTGATGCCACGTTGAAAGGCGGCGTTGACATCAGCCAATACGTCTCAGCTGAATGGATATTGTTCGAGAAAATCAATGGTTTGATAGTGACTGGACGAGGTACATTGGATGGACAAGGTAGTAAGGTTTGGAAGTACAATGATTGCCATGTGAATCAAAATTGTCAGAAGCTCCCAGTTGTAAGGACAAACATGTTTTTTTTTTTTTTTTTTTTTGTCACGTCAAATTCACCGTCTCATTCTTTCCTCGTTCTTCGTTCTTAGAGTTTTAAACTCAATGGCATCCGACACGGACTCGTGAAAGGCCTCACTTCCCTTAACAGTAAAGGGAATCACATGTTCATCACGAATTCCGAAAATATTAGGATTCGAAAGCTACACCTGATCGCACCAGATGATAGTCCGAACACTGATGGTATACATCTTAGTCGCTCCACTAATGTGAGAATTACAAAATCTATCATTGCTACAGGTGACGACTGTATCTCTGTCGGTAGAGGATCGATGAATGTTGTTATCACCCAAATAAAATGCGGGCCAGGACATGGTATCAGGTAATAAAAAAAAAACGTGTTTTGTGTTTTTGTTTAGCTATGTAATATTGCATACGACGTGATGATCAACGAGGGTTCTACTATGACAGTATTGGTAGCCTTGGGAAGTACCCAAATGAGGAGGACGTGCGGGGTATTATCGTTAGGAACTGCACGTTAATTAACACCGACAACGGGCTTAGAATCAAAACATGGGCAGACTCGCCCCCGAGTGCAGCAGTAGGCATACTATTCCAAGACATCAATTTGATCAATGTGAAGAATCCGATCATCATCGACCAACAGTACAGCTGCTCAAAAGTTTGCAAAAAGCCGGTTCGTTATTGTTTTTGTTTTCGTCTTTCGTTTCTCAATCAAACTTAACCCGATTCTAACGTGATTTTATCGGGTACAATGCAGCCATCACGAGTCCGAATCAGCGATGTTCATTTCGTGAATATAAAAGGAACAACAATCTCTCCAGTAGCAGTTAAACTGGTATGCAGCAAGCGGTTCCCATGCCAAAACATTGAGATGTCCAACGTAAATCTAAAGTACAGTGGGCGTCCGAACGACAAGGCGTTGCCGTTTAGGTCAGATTGTACAAATGCAAAAGTAGGCTATAGAGGTCTGCAATTTCCAGCCCCCTGCAAGTGA

mRNA sequence

ATGAATTCTTCTTCGAAACTACCCGTCCTTCTCGGGTTGTTCTTAGCATCATTCGTTGCTGCCAAGGCAAAGGCGCTCATTGGACCGAGCACGCCCGACCCCTTTTCATCAATTAATCTAAACAGTATTACACATCGAAAGGAAGCCGTGTTTAACGTGCTTAGTTTCGGTGCCAAACTCGGTGGCACTAAAGACAACACTCAAACTTTCATGAAGACATGGGTTGCCGCTTGTCGCTCCAAGCAACCGGCTAGAGTTGTTATTCCCAGTGGTGTGTTTTTGATCAATCAAATCGTCTTCGCTGGGCCTTGTCGGAACACTGAACCAATTGTCGTTCAAATTGATGCCACGTTGAAAGGCGGCGTTGACATCAGCCAATACGTCTCAGCTGAATGGATATTGTTCGAGAAAATCAATGGTTTGATAGTGACTGGACGAGGTACATTGGATGGACAAGGTAGTAAGGTTTGGAAGTACAATGATTGCCATGTGAATCAAAATTGTCAGAAGCTCCCAGTTAGTTTTAAACTCAATGGCATCCGACACGGACTCGTGAAAGGCCTCACTTCCCTTAACAGTAAAGGGAATCACATTATTGGTAGCCTTGGGAAGTACCCAAATGAGGAGGACGTGCGGGGTATTATCGTTAGGAACTGCACGTTAATTAACACCGACAACGGGCTTAGAATCAAAACATGGGCAGACTCGCCCCCGAGTGCAGCAGTAGGCATACTATTCCAAGACATCAATTTGATCAATGTGAAGAATCCGATCATCATCGACCAACAGTACAGCTGCTCAAAAGTTTGCAAAAAGCCGCCATCACGAGTCCGAATCAGCGATGTTCATTTCGTGAATATAAAAGGAACAACAATCTCTCCAGTAGCAGTTAAACTGGTATGCAGCAAGCGGTTCCCATGCCAAAACATTGAGATGTCCAACGTAAATCTAAAGTACAGTGGGCGTCCGAACGACAAGGCGTTGCCGTTTAGGTCAGATTGTACAAATGCAAAAGTAGGCTATAGAGGTCTGCAATTTCCAGCCCCCTGCAAGTGA

Coding sequence (CDS)

ATGAATTCTTCTTCGAAACTACCCGTCCTTCTCGGGTTGTTCTTAGCATCATTCGTTGCTGCCAAGGCAAAGGCGCTCATTGGACCGAGCACGCCCGACCCCTTTTCATCAATTAATCTAAACAGTATTACACATCGAAAGGAAGCCGTGTTTAACGTGCTTAGTTTCGGTGCCAAACTCGGTGGCACTAAAGACAACACTCAAACTTTCATGAAGACATGGGTTGCCGCTTGTCGCTCCAAGCAACCGGCTAGAGTTGTTATTCCCAGTGGTGTGTTTTTGATCAATCAAATCGTCTTCGCTGGGCCTTGTCGGAACACTGAACCAATTGTCGTTCAAATTGATGCCACGTTGAAAGGCGGCGTTGACATCAGCCAATACGTCTCAGCTGAATGGATATTGTTCGAGAAAATCAATGGTTTGATAGTGACTGGACGAGGTACATTGGATGGACAAGGTAGTAAGGTTTGGAAGTACAATGATTGCCATGTGAATCAAAATTGTCAGAAGCTCCCAGTTAGTTTTAAACTCAATGGCATCCGACACGGACTCGTGAAAGGCCTCACTTCCCTTAACAGTAAAGGGAATCACATTATTGGTAGCCTTGGGAAGTACCCAAATGAGGAGGACGTGCGGGGTATTATCGTTAGGAACTGCACGTTAATTAACACCGACAACGGGCTTAGAATCAAAACATGGGCAGACTCGCCCCCGAGTGCAGCAGTAGGCATACTATTCCAAGACATCAATTTGATCAATGTGAAGAATCCGATCATCATCGACCAACAGTACAGCTGCTCAAAAGTTTGCAAAAAGCCGCCATCACGAGTCCGAATCAGCGATGTTCATTTCGTGAATATAAAAGGAACAACAATCTCTCCAGTAGCAGTTAAACTGGTATGCAGCAAGCGGTTCCCATGCCAAAACATTGAGATGTCCAACGTAAATCTAAAGTACAGTGGGCGTCCGAACGACAAGGCGTTGCCGTTTAGGTCAGATTGTACAAATGCAAAAGTAGGCTATAGAGGTCTGCAATTTCCAGCCCCCTGCAAGTGA

Protein sequence

MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGIRHGLVKGLTSLNSKGNHIIGSLGKYPNEEDVRGIIVRNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSDCTNAKVGYRGLQFPAPCK

Homology

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BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-63
Identity = 132/373 (35.39%), Postives = 194/373 (52.01%), Query Frame = 0


Query: 49  AVFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTE 108
           +VFNV  +GAK  G  D +Q  MK W AAC S+ P+ V+IP G + + ++   GPC+ ++
Sbjct: 8   SVFNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSK 67

Query: 109 PIVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNC 168
            I  QID  +K   D S++ S  W+ F +I+GL V+G GTLDGQG   W  N+C  N NC
Sbjct: 68  -IGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNC 127

Query: 169 QKLPVSFKLNGIRHGLVKGLTSLNSKGNHI------------------------------ 228
           +   ++ + + ++H +V+ +TSLNSK  HI                              
Sbjct: 128 KHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVG 187

Query: 229 ------------------------------------------IGSLGKYPNEEDVRGIIV 288
                                                     IGSLG+Y NE++VRGI V
Sbjct: 188 ISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITV 247

Query: 289 RNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVC-KKPPS 348
           + CT   T NG+R+KTW +SPP AA  + FQD+ + NV+NP+I+DQ+Y     C ++ PS
Sbjct: 248 KGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPS 307

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BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.1e-57
Identity = 130/378 (34.39%), Postives = 184/378 (48.68%), Query Frame = 0


Query: 50  VFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRN--T 109
           V+++  FGA   G+ +  + F+ TW+  C S  PA +++P G FL   ++FAGPC++  T
Sbjct: 51  VYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVT 110

Query: 110 EPIVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQN 169
             ++  I AT  G      Y + EW LFE+++ L++TG GT  G+G  VWK + C     
Sbjct: 111 VNVIGTIIATTSG------YATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQ 170

Query: 170 CQKLPVSFKLNGIRHGLVKGLTSLNSKGNHI----------------------------- 229
           C   P S K   +++  + G++S+N+K  H+                             
Sbjct: 171 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 230

Query: 230 -------------------------------------------IGSLGKYPNEEDVRGII 289
                                                      +GSLGKY NEEDV GI 
Sbjct: 231 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 290

Query: 290 VRNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPS 348
           V NCT+I TDNGLRIKTW  S PS AV I F++I + +VKNPIIIDQ Y      +   S
Sbjct: 291 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYG----SRGGDS 350

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BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.5e-53
Identity = 122/374 (32.62%), Postives = 179/374 (47.86%), Query Frame = 0


Query: 50  VFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEP 109
           VF++  +GA      D ++  +  +  AC+S  P+ +VIP G F +NQ+   GPC++  P
Sbjct: 26  VFDITKYGA--NSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKS--P 85

Query: 110 IVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQ 169
           + +QI ATLK   D SQ    EW+   K++   ++G G LDGQ +  W   +C  ++ C 
Sbjct: 86  LELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAW---ECKQSKKCN 145

Query: 170 KLPVSFKLNGIRHGLVKGLTSLNSKGNHI------------------------------- 229
           KLP +   N + +  +K +T+L+SK  H+                               
Sbjct: 146 KLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSR 205

Query: 230 -----------------------------------------IGSLGKYPNEEDVRGIIVR 289
                                                    +GSLG  P+E+ V G+ VR
Sbjct: 206 SSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVR 265

Query: 290 NCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKP-PSR 349
           NCT  NTDNG+RIKTW  S P     + F+DI + NV NP++IDQ Y     C K  PS+
Sbjct: 266 NCTFTNTDNGVRIKTWPASHPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQ 325

Query: 350 VRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSDCTN 351
           V+IS V F NIKGT+ +  AV L+ SK  PC+ IE+ ++++ YSG+      P +S C N
Sbjct: 326 VKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEG----PAKSSCEN 385

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BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 6.0e-53
Identity = 121/360 (33.61%), Postives = 171/360 (47.50%), Query Frame = 0


Query: 65  DNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKGGVDI 124
           D+TQ     W  AC S  P+ +++P G F +  I   GPC+++  I +Q+  TLK   D 
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSS--IGLQLQGTLKAPADP 60

Query: 125 SQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVN-QNCQKLPVSFKLNGIRHG 184
           S+     WI   KI+ L + G G  DGQG   W  NDCH N   C+ L ++ +L  + + 
Sbjct: 61  SKIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNS 120

Query: 185 LVKGLTSLNSKGNHI--------------------------------------------- 244
           +++ +T+L+SK  H+                                             
Sbjct: 121 ILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTG 180

Query: 245 ---------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGLRIK 304
                                      +GSLG+Y NEE V GI V+NCT+  + NG+RIK
Sbjct: 181 DDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIK 240

Query: 305 TWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVC-KKPPSRVRISDVHFVNIKGT 351
           TW  S P  A  + FQDI + +V  PI+IDQ Y     C  + PS V++S + F NIKGT
Sbjct: 241 TWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGT 300

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BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 7.8e-53
Identity = 120/371 (32.35%), Postives = 175/371 (47.17%), Query Frame = 0


Query: 53  VLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVV 112
           V  FGAK  G  D ++ F+  W  AC S  P+ VVIP G +L++++   GPC+   PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCK--APIEI 91

Query: 113 QIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLP 172
            +  T++   D S +    W+ F  +    + G G  DGQGS  ++ N C   +   KLP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 173 VSFKLNGIRHGLVKGLTSLNSKGNHI---------------------------------- 232
           V+ + + + + L++ +TS +SK  HI                                  
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 233 --------------------------------------IGSLGKYPNEEDVRGIIVRNCT 292
                                                 IGSLGK+ NEE V GI + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 293 LINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKK-PPSRVRI 351
           + NT NG RIKTW      A   I F+DI + NV +PI+IDQQY     CKK   S+V++
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKL 331

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BLAST of Csor.00g251850 vs. NCBI nr
Match: KAG6597550.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 712 bits (1838), Expect = 5.01e-259
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL
Sbjct: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
           GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG
Sbjct: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI
Sbjct: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHIIGSLGKYPNEEDVRGIIVRNCTLINTDNGLRIKTWADSPPSA 240
           RHGLVKGLTSLNSKGNHIIGSLGKYPNEEDVRGIIVRNCTLINTDNGLRIKTWADSPPSA
Sbjct: 181 RHGLVKGLTSLNSKGNHIIGSLGKYPNEEDVRGIIVRNCTLINTDNGLRIKTWADSPPSA 240

Query: 241 AVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIKGTTISPVAVKLV 300
           AVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIKGTTISPVAVKLV
Sbjct: 241 AVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIKGTTISPVAVKLV 300

Query: 301 CSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSDCTNAKVGYRGLQFPAPCK 351
           CSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSDCTNAKVGYRGLQFPAPCK
Sbjct: 301 CSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSDCTNAKVGYRGLQFPAPCK 351

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BLAST of Csor.00g251850 vs. NCBI nr
Match: XP_022941490.1 (exopolygalacturonase-like [Cucurbita moschata] >KAG7028994.1 Exopolygalacturonase-like 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 674 bits (1738), Expect = 1.28e-242
Identity = 346/423 (81.80%), Postives = 350/423 (82.74%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSI+LNSITHRKEAVFNVLSFGAKL
Sbjct: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSISLNSITHRKEAVFNVLSFGAKL 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
           GGTKDNTQ FMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG
Sbjct: 61  GGTKDNTQNFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           GVD+S+YVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI
Sbjct: 121 GVDVSEYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHI------------------------------------------ 240
           RHGLVKGLTSLNSKGNH+                                          
Sbjct: 181 RHGLVKGLTSLNSKGNHMFITNSENIRVRKLHLIAPDDSPNTDGIHLSRSTNVRITKSII 240

Query: 241 ------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300
                                         IGSLGKYPNEEDVRGIIVRNCTLINTDNGL
Sbjct: 241 ATGDDCISVGRGSMNVVITQIKCGPGHGISIGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300

Query: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 351
           RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK
Sbjct: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 360

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BLAST of Csor.00g251850 vs. NCBI nr
Match: XP_023540892.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658 bits (1698), Expect = 1.59e-236
Identity = 339/423 (80.14%), Postives = 345/423 (81.56%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLP+LLGLFLASFVAA+AKALIGPSTPDPFSSI+L+SI HRKE VFNVLSFGAK 
Sbjct: 1   MNSSSKLPLLLGLFLASFVAAEAKALIGPSTPDPFSSISLDSIIHRKEVVFNVLSFGAKP 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
           GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLI QIVFAGPCRNTEPIVVQIDATLKG
Sbjct: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLIYQIVFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           G D+SQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI
Sbjct: 121 GDDVSQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHI------------------------------------------ 240
           RHGLVKGLTSLNSKGNH+                                          
Sbjct: 181 RHGLVKGLTSLNSKGNHMFITNSENIRVRKLHLIAPDDSPNTDGIHLSRSTNVRITKSTI 240

Query: 241 ------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300
                                         IGSLGKYPNEEDVRGIIVRNCTL NTDNGL
Sbjct: 241 TTGDDCISVGRGSMNVVINQIKCGPGHGISIGSLGKYPNEEDVRGIIVRNCTLFNTDNGL 300

Query: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 351
           RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK
Sbjct: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 360

[Close]

BLAST of Csor.00g251850 vs. NCBI nr
Match: XP_022972202.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 648 bits (1672), Expect = 1.45e-232
Identity = 333/423 (78.72%), Postives = 340/423 (80.38%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLPVLL LFLASFVAA+AK LIGPSTPDPFSSINL+SI HRKE VFNVLSFGAK 
Sbjct: 1   MNSSSKLPVLLWLFLASFVAAEAKVLIGPSTPDPFSSINLDSIRHRKEVVFNVLSFGAKF 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
             TKDNTQTFMKTWVAACRSKQ ARVVIPSGVFLINQI FAGPCRNTEPIVVQIDATLKG
Sbjct: 61  SSTKDNTQTFMKTWVAACRSKQAARVVIPSGVFLINQIAFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           GVD+SQYVSAEWILFEKINGLIVTGRGTLDGQGS+VWKYNDCHVN+NCQKLPVS KLNGI
Sbjct: 121 GVDVSQYVSAEWILFEKINGLIVTGRGTLDGQGSEVWKYNDCHVNENCQKLPVSLKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHI------------------------------------------ 240
           RHGLVKGLTSLNSKGNH+                                          
Sbjct: 181 RHGLVKGLTSLNSKGNHMFITNSENIRVRKLHLIAPDDSPNTDGIHLSRSTKVRITKSTI 240

Query: 241 ------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300
                                         IGSLGKYPNEEDVRGIIVRNCTL NTDNGL
Sbjct: 241 ATGDDCISVGRGSMNVVIDQIKCGPGHGISIGSLGKYPNEEDVRGIIVRNCTLTNTDNGL 300

Query: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 351
           RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK
Sbjct: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 360

[Close]

BLAST of Csor.00g251850 vs. NCBI nr
Match: XP_038877778.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 508 bits (1307), Expect = 5.23e-177
Identity = 263/425 (61.88%), Postives = 297/425 (69.88%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNS-ITHRKEAVFNVLSFGAK 60
           M S+ K  V L L L + VA +AK L GP  P+P  ++NL   I HRKEAVFNVLSFGAK
Sbjct: 1   MKSAFKQVVPLTLLLVALVAVEAKPLAGPILPNPLQNVNLGKDIRHRKEAVFNVLSFGAK 60

Query: 61  LGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLK 120
             G  DNTQ+FMKTW+AAC SK  ARVVIPSG FLINQ+ FAGPC +  PI+VQIDA LK
Sbjct: 61  PDGKTDNTQSFMKTWIAACHSKGAARVVIPSGTFLINQVAFAGPCTSISPIIVQIDAMLK 120

Query: 121 GGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNG 180
           GG D+S YVS EWILFEKINGLIVTGRGTLDGQG  VW+YNDC +N NCQKLPV+ KLNG
Sbjct: 121 GGTDVSNYVSDEWILFEKINGLIVTGRGTLDGQGGAVWQYNDCGMNPNCQKLPVNLKLNG 180

Query: 181 IRHGLVKGLTSLNSKGNHI----------------------------------------- 240
           I HGLVKG++S+NSKG H+                                         
Sbjct: 181 ITHGLVKGISSINSKGFHMFITNCENLRLRKLHITAPVDSPNTDGIHTSRSNNVKISRSV 240

Query: 241 -------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNG 300
                                          IGSLGKYPNEEDVRGIIV+NCTL NTDNG
Sbjct: 241 IATGDDCISLGRGSMNIAINKITCGPGHGISIGSLGKYPNEEDVRGIIVKNCTLTNTDNG 300

Query: 301 LRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKV-CKKPPSRVRISDVHFVN 351
           LRIKTW DSPPSAA GILFQDIN+INVKNPIIIDQ+YSCSK  C+KPPSRV+ISDVH++N
Sbjct: 301 LRIKTWPDSPPSAASGILFQDINMINVKNPIIIDQEYSCSKTNCQKPPSRVKISDVHYIN 360

[Close]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL
Match: A0A6J1FTU0 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446770 PE=3 SV=1)

HSP 1 Score: 674 bits (1738), Expect = 6.21e-243
Identity = 346/423 (81.80%), Postives = 350/423 (82.74%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSI+LNSITHRKEAVFNVLSFGAKL
Sbjct: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSISLNSITHRKEAVFNVLSFGAKL 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
           GGTKDNTQ FMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG
Sbjct: 61  GGTKDNTQNFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           GVD+S+YVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI
Sbjct: 121 GVDVSEYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHI------------------------------------------ 240
           RHGLVKGLTSLNSKGNH+                                          
Sbjct: 181 RHGLVKGLTSLNSKGNHMFITNSENIRVRKLHLIAPDDSPNTDGIHLSRSTNVRITKSII 240

Query: 241 ------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300
                                         IGSLGKYPNEEDVRGIIVRNCTLINTDNGL
Sbjct: 241 ATGDDCISVGRGSMNVVITQIKCGPGHGISIGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300

Query: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 351
           RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK
Sbjct: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 360

[Close]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL
Match: A0A6J1I470 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470806 PE=3 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 7.01e-233
Identity = 333/423 (78.72%), Postives = 340/423 (80.38%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNSITHRKEAVFNVLSFGAKL 60
           MNSSSKLPVLL LFLASFVAA+AK LIGPSTPDPFSSINL+SI HRKE VFNVLSFGAK 
Sbjct: 1   MNSSSKLPVLLWLFLASFVAAEAKVLIGPSTPDPFSSINLDSIRHRKEVVFNVLSFGAKF 60

Query: 61  GGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLKG 120
             TKDNTQTFMKTWVAACRSKQ ARVVIPSGVFLINQI FAGPCRNTEPIVVQIDATLKG
Sbjct: 61  SSTKDNTQTFMKTWVAACRSKQAARVVIPSGVFLINQIAFAGPCRNTEPIVVQIDATLKG 120

Query: 121 GVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNGI 180
           GVD+SQYVSAEWILFEKINGLIVTGRGTLDGQGS+VWKYNDCHVN+NCQKLPVS KLNGI
Sbjct: 121 GVDVSQYVSAEWILFEKINGLIVTGRGTLDGQGSEVWKYNDCHVNENCQKLPVSLKLNGI 180

Query: 181 RHGLVKGLTSLNSKGNHI------------------------------------------ 240
           RHGLVKGLTSLNSKGNH+                                          
Sbjct: 181 RHGLVKGLTSLNSKGNHMFITNSENIRVRKLHLIAPDDSPNTDGIHLSRSTKVRITKSTI 240

Query: 241 ------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNGL 300
                                         IGSLGKYPNEEDVRGIIVRNCTL NTDNGL
Sbjct: 241 ATGDDCISVGRGSMNVVIDQIKCGPGHGISIGSLGKYPNEEDVRGIIVRNCTLTNTDNGL 300

Query: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 351
           RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK
Sbjct: 301 RIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPSRVRISDVHFVNIK 360

[Close]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL
Match: A0A5D3CWB7 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001250 PE=3 SV=1)

HSP 1 Score: 504 bits (1297), Expect = 8.38e-176
Identity = 261/425 (61.41%), Postives = 299/425 (70.35%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNS-ITHRKEAVFNVLSFGAK 60
           M+S  K  +LL L L + VA  AK L GP  PDPF ++NL   I HRKE VFNVLSFGAK
Sbjct: 1   MSSFFKQVILLTLLLVALVAVDAKLLPGPILPDPFRNVNLGKDIKHRKETVFNVLSFGAK 60

Query: 61  LGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLK 120
             G  DNT++FMKTWVAAC SK  ARVVIPSG FLINQ+ FAGPC +T PIVVQIDATLK
Sbjct: 61  PDGKTDNTESFMKTWVAACHSKGAARVVIPSGTFLINQVAFAGPCASTSPIVVQIDATLK 120

Query: 121 GGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNG 180
           GG+DIS Y S EWILFEKINGLIVTGRGTLDGQGS VW+YNDC +N NCQKLPV+ KL+G
Sbjct: 121 GGIDISNYFSDEWILFEKINGLIVTGRGTLDGQGSAVWEYNDCGMNPNCQKLPVNLKLSG 180

Query: 181 IRHGLVKGLTSLNSKGNHI----------------------------------------- 240
           I HGLVKG++S+NSKG H+                                         
Sbjct: 181 ITHGLVKGISSVNSKGFHMFITNCENVRLRKLHITAPETSPNTDGIHVSRSNNVKISRSV 240

Query: 241 -------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNG 300
                                          +GSLGKY NEEDVRGIIV+NCTL+ TDNG
Sbjct: 241 IATGDDCISLGRGSMNIAINKITCGPGHGISVGSLGKYQNEEDVRGIIVKNCTLVKTDNG 300

Query: 301 LRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKV-CKKPPSRVRISDVHFVN 351
           LRIKTW DSPPSAA GILFQDIN+I+VKNPI+IDQ+YSCSK  C+KPPSRV+ISDVH++N
Sbjct: 301 LRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQKPPSRVKISDVHYIN 360

[Close]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL
Match: A0A0A0LAM9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164550 PE=3 SV=1)

HSP 1 Score: 504 bits (1297), Expect = 8.38e-176
Identity = 259/425 (60.94%), Postives = 299/425 (70.35%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNS-ITHRKEAVFNVLSFGAK 60
           M+S  K  +LL L L + VA  AK L GP +PDPF ++NL   I HRKE VFNVLSFGAK
Sbjct: 1   MSSVFKQLILLTLLLVALVAVDAKLLAGPISPDPFLNVNLGKDIKHRKETVFNVLSFGAK 60

Query: 61  LGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLK 120
             G  DN+++FMKTWVAAC SK  ARVVIP G FLINQI FAGPC +T PI+VQIDATLK
Sbjct: 61  PDGKTDNSESFMKTWVAACHSKGAARVVIPRGTFLINQIAFAGPCASTSPIIVQIDATLK 120

Query: 121 GGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNG 180
           GGVDIS Y S EWILFEKINGLIVTGRG LDGQGS VW+YNDC +N NCQKLPV+ KLN 
Sbjct: 121 GGVDISNYFSDEWILFEKINGLIVTGRGALDGQGSAVWEYNDCGMNPNCQKLPVNLKLNN 180

Query: 181 IRHGLVKGLTSLNSKGNHI----------------------------------------- 240
           I HGLVKG++S+NSKG H+                                         
Sbjct: 181 ITHGLVKGISSVNSKGFHMFVTNCENVRLRKLHLTAPEDSPNTDGIHLSRSNNIKISRSV 240

Query: 241 -------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNG 300
                                          +GSLGKYPNEEDVRGI+V+NCTL++TDNG
Sbjct: 241 IATGDDCISLGRGSMNVAINKITCGPGHGISVGSLGKYPNEEDVRGIVVKNCTLLSTDNG 300

Query: 301 LRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKV-CKKPPSRVRISDVHFVN 351
           LRIKTW DSPPSAA GILFQDIN++NVKNPI+IDQ+YSCSK  C++PPSRV+ISDVH++N
Sbjct: 301 LRIKTWPDSPPSAASGILFQDINMVNVKNPILIDQEYSCSKTNCQRPPSRVKISDVHYIN 360

[Close]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL
Match: A0A1S3AXH3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103483868 PE=3 SV=1)

HSP 1 Score: 504 bits (1297), Expect = 8.38e-176
Identity = 261/425 (61.41%), Postives = 299/425 (70.35%), Query Frame = 0


Query: 1   MNSSSKLPVLLGLFLASFVAAKAKALIGPSTPDPFSSINLNS-ITHRKEAVFNVLSFGAK 60
           M+S  K  +LL L L + VA  AK L GP  PDPF ++NL   I HRKE VFNVLSFGAK
Sbjct: 1   MSSFFKQVILLTLLLVALVAVDAKLLPGPILPDPFRNVNLGKDIKHRKETVFNVLSFGAK 60

Query: 61  LGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTEPIVVQIDATLK 120
             G  DNT++FMKTWVAAC SK  ARVVIPSG FLINQ+ FAGPC +T PIVVQIDATLK
Sbjct: 61  PDGKTDNTESFMKTWVAACHSKGAARVVIPSGTFLINQVAFAGPCASTSPIVVQIDATLK 120

Query: 121 GGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNCQKLPVSFKLNG 180
           GG+DIS Y S EWILFEKINGLIVTGRGTLDGQGS VW+YNDC +N NCQKLPV+ KL+G
Sbjct: 121 GGIDISNYFSDEWILFEKINGLIVTGRGTLDGQGSAVWEYNDCGMNPNCQKLPVNLKLSG 180

Query: 181 IRHGLVKGLTSLNSKGNHI----------------------------------------- 240
           I HGLVKG++S+NSKG H+                                         
Sbjct: 181 ITHGLVKGISSVNSKGFHMFITNCENVRLRKLHITAPETSPNTDGIHVSRSNNVKISRSV 240

Query: 241 -------------------------------IGSLGKYPNEEDVRGIIVRNCTLINTDNG 300
                                          +GSLGKY NEEDVRGIIV+NCTL+ TDNG
Sbjct: 241 IATGDDCISLGRGSMNIAINKITCGPGHGISVGSLGKYQNEEDVRGIIVKNCTLVKTDNG 300

Query: 301 LRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKV-CKKPPSRVRISDVHFVN 351
           LRIKTW DSPPSAA GILFQDIN+I+VKNPI+IDQ+YSCSK  C+KPPSRV+ISDVH++N
Sbjct: 301 LRIKTWPDSPPSAASGILFQDINMIDVKNPILIDQEYSCSKTNCQKPPSRVKISDVHYIN 360

[Close]

BLAST of Csor.00g251850 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 8.9e-60
Identity = 135/391 (34.53%), Postives = 197/391 (50.38%), Query Frame = 0


Query: 47  KEAVF--NVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPA-RVVIPSGVFLINQIVFAGP 106
           K +VF  +V SFGA+    +D+T+ F+  W  AC+S   +  ++IP G F +  + F+GP
Sbjct: 29  KSSVFLVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGP 88

Query: 107 CRNTEPIVVQIDATLKGGVDISQYVS-AEWILFEKINGLIVTGRGTLDGQGSKVWKYNDC 166
           C N   + V++    K   D+S+Y S   WI F  INGL +TG GT DGQG+  W +N+C
Sbjct: 89  CTNVSNLTVRV----KASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNC 148

Query: 167 HVNQNCQKLPVSFKLNGIRHGLVKGLTSLNSKGNHI------------------------ 226
             + NC+ LP S K  G+   +V+ ++S+NSK  HI                        
Sbjct: 149 TSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNT 208

Query: 227 ------------------------------------------------IGSLGKYPNEED 286
                                                           +GSLG+YPNE+D
Sbjct: 209 DGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKD 268

Query: 287 VRGIIVRNCTLINTDNGLRIKTWADSPP-SAAVGILFQDINLINVKNPIIIDQQYSCSKV 346
           V G++V++C +  T NG+RIKTWA+SP  SAA  + F++I + NV NPIIIDQ Y     
Sbjct: 269 VNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSS 328

Query: 347 C-KKPPSRVRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNL---------KY 351
           C    PS+V +S+++F NI+GT+ S VAV+L CS+  PC+ + + NV+L         K 
Sbjct: 329 CISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQ 388

[Close]

BLAST of Csor.00g251850 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-58
Identity = 130/378 (34.39%), Postives = 184/378 (48.68%), Query Frame = 0


Query: 50  VFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRN--T 109
           V+++  FGA   G+ +  + F+ TW+  C S  PA +++P G FL   ++FAGPC++  T
Sbjct: 51  VYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVT 110

Query: 110 EPIVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQN 169
             ++  I AT  G      Y + EW LFE+++ L++TG GT  G+G  VWK + C     
Sbjct: 111 VNVIGTIIATTSG------YATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQ 170

Query: 170 CQKLPVSFKLNGIRHGLVKGLTSLNSKGNHI----------------------------- 229
           C   P S K   +++  + G++S+N+K  H+                             
Sbjct: 171 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 230

Query: 230 -------------------------------------------IGSLGKYPNEEDVRGII 289
                                                      +GSLGKY NEEDV GI 
Sbjct: 231 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 290

Query: 290 VRNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKKPPS 348
           V NCT+I TDNGLRIKTW  S PS AV I F++I + +VKNPIIIDQ Y      +   S
Sbjct: 291 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYG----SRGGDS 350

[Close]

BLAST of Csor.00g251850 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-54
Identity = 129/379 (34.04%), Postives = 181/379 (47.76%), Query Frame = 0


Query: 50  VFNVLSFGAKLGGTKDNTQTFMKTWVAACR-SKQPARVVIPSGVFLINQIVFAGPCRNTE 109
           +F+V ++GA+    +DN   F K W  AC+ S   + V IPSG+F + Q+ F+GPC+++ 
Sbjct: 31  IFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSGIFYLRQVTFSGPCKSS- 90

Query: 110 PIVVQIDATLKGGVDISQYVSAEWILFEKINGLIVTGRGTLDGQGSKVWKYNDCHVNQNC 169
            I   I  TL    +       EWILF+ ++ L VTG G LDGQGS  W  NDC+ N NC
Sbjct: 91  -ITFFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGGGLLDGQGSYSWPLNDCNKNTNC 150

Query: 170 QKLPVSFKLNGIRHGLVKGLTSLNSKGNHI------------------------------ 229
           + L ++     ++   + GL S+NSK  H                               
Sbjct: 151 RTLAMNIGFAFVKSSKINGLRSINSKMGHFNLFSVEDFNITGVTITAPGDSPNTDGIKIG 210

Query: 230 ------------------------------------------IGSLGKYPNEEDVRGIIV 289
                                                     +GSLG+Y  E++V+G+ V
Sbjct: 211 KSSHMQIYNVTIGTGDDCIAILDGTSNLDISDVRCGPGHGISVGSLGRYKEEKNVQGLTV 270

Query: 290 RNCTLINTDNGLRIKTWADSPPSAAV-GILFQDINLINVKNPIIIDQQYSCSKVCKKP-- 349
           RN  +  T +GLRIKTWA S    +V   L+++I +INV NPI+IDQQY     C  P  
Sbjct: 271 RNSIINGTTDGLRIKTWAKSVSQISVSNFLYENIQMINVGNPIVIDQQYCPHGQCDSPGK 330

Query: 350 -PSRVRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNLKYSGRPNDKALPFRS 352
             S V+I DV + NI GT+ S  A+K+ CSK FPCQ++E+SN+NL Y GR         +
Sbjct: 331 YASHVQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINLHYVGRDG----LVTA 390

[Close]

BLAST of Csor.00g251850 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-54
Identity = 126/377 (33.42%), Postives = 179/377 (47.48%), Query Frame = 0


Query: 49  AVFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTE 108
           A  +V + GAK  G  D++  F   W  AC +   + + +P G +L+  + F GPC+   
Sbjct: 69  ASLDVKASGAKGDGKTDDSAAFAAAWKEACAA--GSTITVPKGEYLVESLEFKGPCKG-- 128

Query: 109 PIVVQIDATLKGGVDI-SQYVSAEWILFEKINGLIVTG-RGTLDGQGSKVWKYNDCHVNQ 168
           P+ ++++   K    + +    A WI FE +    + G +   DGQGS  WK NDC    
Sbjct: 129 PVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTG 188

Query: 169 NCQKLPVSFKLNGIRHGLVKGLTSLNSK-----------------------------GNH 228
            C  LP++ +  G+ +  +  +TS NSK                             G H
Sbjct: 189 KCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIH 248

Query: 229 I-------------------------------------------IGSLGKYPNEEDVRGI 288
           I                                           IGSLG+YPNE+ V+G+
Sbjct: 249 IGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGV 308

Query: 289 IVRNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKK-P 348
            VR C + NTDNG+RIKTW  SPP  A  ILF+DI + NV  P++IDQ+Y     CK   
Sbjct: 309 TVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGV 368

Query: 349 PSRVRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSD 351
           PS+V++SDV   NIKGT+ + VAVKL+CSK  PC NI +S++NL ++G+      P  S 
Sbjct: 369 PSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG----PAVSA 428

[Close]

BLAST of Csor.00g251850 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 6.2e-53
Identity = 124/377 (32.89%), Postives = 177/377 (46.95%), Query Frame = 0


Query: 49  AVFNVLSFGAKLGGTKDNTQTFMKTWVAACRSKQPARVVIPSGVFLINQIVFAGPCRNTE 108
           A  +V + GAK     D++  F   W  AC +   + + +P G +++  + F GPC+   
Sbjct: 68  AAVDVKASGAKGDSKTDDSAAFAAAWKEACAA--GSTITVPKGEYMVESLEFKGPCKG-- 127

Query: 109 PIVVQIDATLKGGVDI-SQYVSAEWILFEKINGLIVTG-RGTLDGQGSKVWKYNDCHVNQ 168
           P+ ++++   K    + +    A WI FE I    + G +   DGQGS  WK NDC    
Sbjct: 128 PVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTG 187

Query: 169 NCQKLPVSFKLNGIRHGLVKGLTSLNSK-----------------------------GNH 228
            C  LP++ +  G+ +  +  +TS NSK                             G H
Sbjct: 188 KCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIH 247

Query: 229 I-------------------------------------------IGSLGKYPNEEDVRGI 288
           I                                           IGSLG+YPNE+ V+G+
Sbjct: 248 IGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGV 307

Query: 289 IVRNCTLINTDNGLRIKTWADSPPSAAVGILFQDINLINVKNPIIIDQQYSCSKVCKK-P 348
            VR C + NTDNG+RIKTW  SPP  A  ILF+DI + NV  P++IDQ+Y     CK   
Sbjct: 308 TVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGV 367

Query: 349 PSRVRISDVHFVNIKGTTISPVAVKLVCSKRFPCQNIEMSNVNLKYSGRPNDKALPFRSD 351
           PS+V++SDV    IKGT+ + VAVKL+CSK  PC NI +S++NL ++G+      P  S 
Sbjct: 368 PSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG----PAVSA 427

The following BLAST results are available for this feature:

BLAST of Csor.00g251850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6H9K0	1.3e-63	35.39	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P49062	3.1e-57	34.39	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q05967	3.5e-53	32.62	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
P24548	6.0e-53	33.61	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q39786	7.8e-53	32.35	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]

BLAST of Csor.00g251850 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6597550.1	5.01e-259	100.00	Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022941490.1	1.28e-242	81.80	exopolygalacturonase-like [Cucurbita moschata] >KAG7028994.1 Exopolygalacturonas...	[more]
XP_023540892.1	1.59e-236	80.14	exopolygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022972202.1	1.45e-232	78.72	exopolygalacturonase-like [Cucurbita maxima]	[more]
XP_038877778.1	5.23e-177	61.88	exopolygalacturonase-like [Benincasa hispida]	[more]

BLAST of Csor.00g251850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTU0	6.21e-243	81.80	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446770 PE=3 SV=...	[more]
A0A6J1I470	7.01e-233	78.72	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470806 PE=3 SV=1	[more]
A0A5D3CWB7	8.38e-176	61.41	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A0A0LAM9	8.38e-176	60.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164550 PE=3 SV=1	[more]
A0A1S3AXH3	8.38e-176	61.41	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103483868 PE=3 SV=1	[more]

BLAST of Csor.00g251850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18180.1	8.9e-60	34.53	Pectin lyase-like superfamily protein	[more]
AT1G02790.1	2.2e-58	34.39	polygalacturonase 4	[more]
AT2G33160.1	2.5e-54	34.04	glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro...	[more]
AT3G14040.1	2.5e-54	33.42	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	6.2e-53	32.89	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 199..337 e-value: 7.5E-37 score: 127.1 coord: 78..198 e-value: 7.6E-20 score: 71.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 37..199 e-value: 3.4E-29 score: 104.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 200..328 e-value: 1.8E-38 score: 134.2
None	No IPR available	PANTHER	PTHR31375:SF117	EXOPOLYGALACTURONASE-LIKE	coord: 33..198 coord: 199..350
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 33..198 coord: 199..350
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 45..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g251850.m01	Csor.00g251850.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity