Csor.00g251100 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g251100 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-121
Identity = 245/460 (53.26%), Postives = 302/460 (65.65%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSS---SSSTAA 60
           M H     N  P    F     S P S+     PKR    +SSSSSS  +    +S  A+
Sbjct: 9   MKHAPFLRNRSPQQTLFIPYTLSLPISYQN---PKRLKTANSSSSSSLLAPVILNSPVAS 68

Query: 61  ASTAMVH-----------KNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVA 120
           +S   ++               ++ +WR+I GCNNW+DL+EP L+PLL++E+ RYG  V+
Sbjct: 69  SSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLVS 128

Query: 121 ACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYIYATPPF-INIPPIQNSP 180
            CYKAFDLDPNSKRYL CK+GK+ L KE  ++    Y+V+KYIYATP   INI PIQN  
Sbjct: 129 TCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEM 188

Query: 181 RACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPD 240
               RW+GYVA SSD    RLGRRDIV+TFRGTVT PEW+A  MSSLTPA    +N R D
Sbjct: 189 NRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLD 248

Query: 241 VKVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGV 300
           VKV SGFL LYT +E  +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS+L  
Sbjct: 249 VKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQ 308

Query: 301 LLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELGLN+R  K  +P++VFSF GP+VGNL FK+RCEELGVKVLRI NVNDP+TK
Sbjct: 309 LLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTK 368

Query: 361 MPGVLFNS-----------------YEHVGVELVLEFFNMQNPSWVHDLETYISLLKYQE 420
           +PGVLFN                  Y HVGVEL L+FF++QN S VHDL+TYI LL  + 
Sbjct: 369 LPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLLNQRR 428

Query: 421 DDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFLCNNAQNL 428
            +  S +SD D+D             N A++FL  N + +
Sbjct: 429 TNSRSVDSDEDEDS-----------DNVALEFLKTNGEKM 453

BLAST of Csor.00g251100 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.7e-119
Identity = 233/390 (59.74%), Postives = 286/390 (73.33%), Query Frame = 0

Query: 13  NHFTFSD-TIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAASTAMVHKNAGMTS 72
           +HF+ S  T +    S   +S P  S  +S  SSSSS +   S A A T        ++ 
Sbjct: 29  DHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAVT------LPLSR 88

Query: 73  LWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNL 132
           +WR+I G NNWE+L+EP L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKKNL
Sbjct: 89  VWREIQGSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNL 148

Query: 133 FKEVGLEN-SGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRRDI 192
            KE G+ +  GY+V+KYIYATP  IN+ PI+N P    RWIGYVAVSSD    RLGRRDI
Sbjct: 149 LKESGIHDPDGYQVTKYIYATPD-INLNPIKNEPNR-ARWIGYVAVSSDESVKRLGRRDI 208

Query: 193 VITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEKCTKFGLESCR 252
           ++TFRGTVT  EW+A L SSLTPA LD +N RPDVKV SGFL LYT  E  +KFGLESCR
Sbjct: 209 LVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCR 268

Query: 253 EQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFS 312
           EQLLSE+SRL+NK+K EE+SIT+AGHSMGS+L  LLAYDIAELG+N+R +++ VP++VFS
Sbjct: 269 EQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFS 328

Query: 313 FGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS-----------------Y 372
           F GP+VGNL FK+RCEELGVKVLRI NVNDPITK+PG LFN                  Y
Sbjct: 329 FAGPRVGNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCY 388

Query: 373 EHVGVELVLEFFNMQNPSWVHDLETYISLL 384
            HVGVEL L+FF++QN S VHDLETYI+L+
Sbjct: 389 THVGVELTLDFFDVQNISCVHDLETYITLV 409

BLAST of Csor.00g251100 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 4.9e-105
Identity = 190/332 (57.23%), Postives = 242/332 (72.89%), Query Frame = 0

Query: 69  MTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGK 128
           +  +WR++ GC +WE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 129 KNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRR 188
           + + +EVG+  +GYEV++YIYA    +++P ++ S    GRWIGYVAVS+D M+ RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAAAD-VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 189 DIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEKCTKF-GLE 248
           D++++FRGTVT  EW+A LMSSL  A LD  + RPDVKV SGFL LYT  +K  +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 249 SCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVP 308
           SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSAL +L AYD+AELGLN+     A P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 309 ISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------------- 368
           ++VFSFGGP+VGN  FK RC+ELGVK LR+ NV+DPITK+PGV  N              
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

Query: 369 YEHVGVELVLEFFNMQNPSWVHDLETYISLLK 385
           Y HVGVEL L+FF + + + VHDL TYISLL+
Sbjct: 356 YTHVGVELPLDFFKVGDLASVHDLATYISLLR 381

BLAST of Csor.00g251100 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 4.9e-105
Identity = 190/332 (57.23%), Postives = 242/332 (72.89%), Query Frame = 0

Query: 69  MTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGK 128
           +  +WR++ GC +WE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 129 KNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRR 188
           + + +EVG+  +GYEV++YIYA    +++P ++ S    GRWIGYVAVS+D M+ RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAAAD-VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 189 DIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEKCTKF-GLE 248
           D++++FRGTVT  EW+A LMSSL  A LD  + RPDVKV SGFL LYT  +K  +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 249 SCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVP 308
           SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSAL +L AYD+AELGLN+     A P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 309 ISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------------- 368
           ++VFSFGGP+VGN  FK RC+ELGVK LR+ NV+DPITK+PGV  N              
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

Query: 369 YEHVGVELVLEFFNMQNPSWVHDLETYISLLK 385
           Y HVGVEL L+FF + + + VHDL TYISLL+
Sbjct: 356 YTHVGVELPLDFFKVGDLASVHDLATYISLLR 381

BLAST of Csor.00g251100 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 3.2e-72
Identity = 164/388 (42.27%), Postives = 226/388 (58.25%), Query Frame = 0

Query: 73  WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLF 132
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +   F
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 133 KEVGLENSGYEVSKYIYATPPFINIPPIQNSPR------ACGRWIGYVAVSSDYMT-TRL 192
             +G+ +SGYEV++Y+YAT   IN+P   +  R          W+GYVAVS D  +  RL
Sbjct: 165 DSLGMIDSGYEVARYLYATSN-INLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 193 GRRDIVITFRGTVTIPEWIAILMSSLTPA---HLDCYNYRPDVKVLSGFLMLYTCNEKCT 252
           GRRDI I +RGTVT  EWIA L   L P     + C +  P VKV SGFL LYT  +   
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPD--PAVKVESGFLDLYTDKDTTC 284

Query: 253 KFGLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALGVLLAYDIAELGLNKRT 312
           KF   S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G AL +L AYDIAE+ LN+  
Sbjct: 285 KFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSK 344

Query: 313 NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------- 372
             + +P++V ++GGP+VGN+ F++R EELGVKV+R+VNV+D + K PG+  N        
Sbjct: 345 KGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALM 404

Query: 373 ---------YEHVGVELVLEFFNMQ------NPSWVHDLETYISLLKYQEDDDDSDNSDG 426
                    Y HVG EL L+  N        + S  H+LE  + LL       D  +  G
Sbjct: 405 KIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL-------DGYHGKG 464

BLAST of Csor.00g251100 vs. NCBI nr
Match: KAG6597624.1 (Phospholipase A1-Ialpha2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029065.1 Phospholipase A1-Ialpha2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860 bits (2223), Expect = 5.36e-315
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK
Sbjct: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL 420
           VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL 420

Query: 421 CNNAQNLRIG 430
           CNNAQNLRIG
Sbjct: 421 CNNAQNLRIG 430

BLAST of Csor.00g251100 vs. NCBI nr
Match: XP_023540584.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813 bits (2100), Expect = 2.40e-296
Identity = 406/427 (95.08%), Postives = 410/427 (96.02%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSS          TAAA  
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSS----------TAAA-- 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSP ACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           M+TRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL LYTCNEK
Sbjct: 181 MSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL 420
           VLEFFNMQNPSWVHDLETYISLL+Y EDDDDSDNSD DDDGYGSGR+KIIIVINKAM+FL
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLRYPEDDDDSDNSDRDDDGYGSGRRKIIIVINKAMEFL 415

Query: 421 CNNAQNL 427
           C NAQNL
Sbjct: 421 CTNAQNL 415

BLAST of Csor.00g251100 vs. NCBI nr
Match: XP_022932577.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 813 bits (2101), Expect = 2.56e-296
Identity = 405/428 (94.63%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSS++        AAAST
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSTA--------AAAST 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAGMTSLWRQ+HGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGMTSLWRQLHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSP ACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL LYTCNEK
Sbjct: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSE+SRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEISRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRI NVNDPITKMPGVLFNSYEHVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIANVNDPITKMPGVLFNSYEHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDD-SDNSDGDDDGYGSGRQKIIIVINKAMDF 420
           VLEFFNMQNPSWVHDLETYISLL+Y+EDDDD SDNSDGDDDGY SGR+KI+IVINKAMDF
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLRYREDDDDDSDNSDGDDDGYVSGRRKIVIVINKAMDF 420

Query: 421 LCNNAQNL 427
           LC+NA NL
Sbjct: 421 LCSNAPNL 420

BLAST of Csor.00g251100 vs. NCBI nr
Match: XP_022972209.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 785 bits (2027), Expect = 3.85e-285
Identity = 389/427 (91.10%), Postives = 403/427 (94.38%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSN HSFGQVSLPKRSDNVSSSSS++             
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNSHSFGQVSLPKRSDNVSSSSSTA------------- 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAG TSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGFTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFI+IPPIQNSP ACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFIHIPPIQNSPPACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           M+ RLGRRDIVITFRGTVTIPEWIAIL SSLTPAHLDCY+YRPDVKV+SGFL LYTC EK
Sbjct: 181 MSKRLGRRDIVITFRGTVTIPEWIAILTSSLTPAHLDCYSYRPDVKVVSGFLTLYTCEEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNL+FK+RCEELGVKVLRIVNVNDPITKMPGVLFNSY+HVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLSFKERCEELGVKVLRIVNVNDPITKMPGVLFNSYKHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL 420
           VLEFFNMQNPSWVHDLETYISLL+Y +DDDDSDNSDGDDDGY   R+KIIIVINKAM+FL
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLRYPKDDDDSDNSDGDDDGYDDRRRKIIIVINKAMEFL 414

Query: 421 CNNAQNL 427
           C+NAQNL
Sbjct: 421 CSNAQNL 414

BLAST of Csor.00g251100 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 632 bits (1631), Expect = 5.67e-224
Identity = 344/476 (72.27%), Postives = 374/476 (78.57%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSD-NVSSSSSSSSSSS-------- 60
           MA TTLKLNSIPNH TF +T R   HSFGQVS+P++SD NVSSSSSSSSSSS        
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  -----------------SSSTAAASTAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLL 120
                            SS + AA+ AMV K A +  LWRQIHGCN+WEDLVEP LHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPF 180
           RRE+IRYGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP 
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPA 240
           INIPPIQNSP +CGRWIGYVAVSSD  + RLGRRDI+ITFRGTVT PEWIA LMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 HLDCYNYRPDVKVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
            LD +N+RPDVKV SGFL LYT  E   KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLR 360
           GHSMGSAL +LLAYDIAELGLN+RTN E VP+SVFSFGGP+VGN  FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFN----------------SYEHVGVELVLEFFNMQNPSWVHDLET 420
           IVNVNDPITKMPGVLFN                SYEHVGVELVL+FFNMQNPS VHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLL---KYQEDDDDSDNSDGDDDGYGSGRQKIII----VINKAMDFLCNNAQNL 427
           YISLL   K QED+D+ ++ D       SGR KIII     INKAM+FLC+NAQ+L
Sbjct: 421 YISLLRCPKKQEDEDEDEDED-------SGRGKIIINSGEFINKAMEFLCSNAQSL 469

BLAST of Csor.00g251100 vs. ExPASy TrEMBL
Match: A0A6J1EX59 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439091 PE=4 SV=1)

HSP 1 Score: 813 bits (2101), Expect = 1.24e-296
Identity = 405/428 (94.63%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSS++        AAAST
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSTA--------AAAST 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAGMTSLWRQ+HGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGMTSLWRQLHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSP ACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL LYTCNEK
Sbjct: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSE+SRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEISRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRI NVNDPITKMPGVLFNSYEHVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIANVNDPITKMPGVLFNSYEHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDD-SDNSDGDDDGYGSGRQKIIIVINKAMDF 420
           VLEFFNMQNPSWVHDLETYISLL+Y+EDDDD SDNSDGDDDGY SGR+KI+IVINKAMDF
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLRYREDDDDDSDNSDGDDDGYVSGRRKIVIVINKAMDF 420

Query: 421 LCNNAQNL 427
           LC+NA NL
Sbjct: 421 LCSNAPNL 420

BLAST of Csor.00g251100 vs. ExPASy TrEMBL
Match: A0A6J1IAU6 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111470810 PE=4 SV=1)

HSP 1 Score: 785 bits (2027), Expect = 1.87e-285
Identity = 389/427 (91.10%), Postives = 403/427 (94.38%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAAST 60
           MAHTTLKLNSIPNHFTFSDTIRSN HSFGQVSLPKRSDNVSSSSS++             
Sbjct: 1   MAHTTLKLNSIPNHFTFSDTIRSNSHSFGQVSLPKRSDNVSSSSSTA------------- 60

Query: 61  AMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120
           AMVHKNAG TSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR
Sbjct: 61  AMVHKNAGFTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKR 120

Query: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDY 180
           YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFI+IPPIQNSP ACGRWIGYVAVSSDY
Sbjct: 121 YLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFIHIPPIQNSPPACGRWIGYVAVSSDY 180

Query: 181 MTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEK 240
           M+ RLGRRDIVITFRGTVTIPEWIAIL SSLTPAHLDCY+YRPDVKV+SGFL LYTC EK
Sbjct: 181 MSKRLGRRDIVITFRGTVTIPEWIAILTSSLTPAHLDCYSYRPDVKVVSGFLTLYTCEEK 240

Query: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN
Sbjct: 241 CTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL 360
           KEAVPISVFSFGGPKVGNL+FK+RCEELGVKVLRIVNVNDPITKMPGVLFNSY+HVGVEL
Sbjct: 301 KEAVPISVFSFGGPKVGNLSFKERCEELGVKVLRIVNVNDPITKMPGVLFNSYKHVGVEL 360

Query: 361 VLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFL 420
           VLEFFNMQNPSWVHDLETYISLL+Y +DDDDSDNSDGDDDGY   R+KIIIVINKAM+FL
Sbjct: 361 VLEFFNMQNPSWVHDLETYISLLRYPKDDDDSDNSDGDDDGYDDRRRKIIIVINKAMEFL 414

Query: 421 CNNAQNL 427
           C+NAQNL
Sbjct: 421 CSNAQNL 414

BLAST of Csor.00g251100 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 632 bits (1631), Expect = 2.74e-224
Identity = 344/476 (72.27%), Postives = 374/476 (78.57%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSD-NVSSSSSSSSSSS-------- 60
           MA TTLKLNSIPNH TF +T R   HSFGQVS+P++SD NVSSSSSSSSSSS        
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  -----------------SSSTAAASTAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLL 120
                            SS + AA+ AMV K A +  LWRQIHGCN+WEDLVEP LHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPF 180
           RRE+IRYGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP 
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPA 240
           INIPPIQNSP +CGRWIGYVAVSSD  + RLGRRDI+ITFRGTVT PEWIA LMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 HLDCYNYRPDVKVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
            LD +N+RPDVKV SGFL LYT  E   KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLR 360
           GHSMGSAL +LLAYDIAELGLN+RTN E VP+SVFSFGGP+VGN  FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFN----------------SYEHVGVELVLEFFNMQNPSWVHDLET 420
           IVNVNDPITKMPGVLFN                SYEHVGVELVL+FFNMQNPS VHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLL---KYQEDDDDSDNSDGDDDGYGSGRQKIII----VINKAMDFLCNNAQNL 427
           YISLL   K QED+D+ ++ D       SGR KIII     INKAM+FLC+NAQ+L
Sbjct: 421 YISLLRCPKKQEDEDEDEDED-------SGRGKIIINSGEFINKAMEFLCSNAQSL 469

BLAST of Csor.00g251100 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 9.30e-224
Identity = 334/441 (75.74%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSS------SSSSSSS----- 60
           MA TTLKLN   NH TFS+T R   HSFGQVSLP++SD+VSS      SSSSSSS     
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  ----SSSSSTAAASTAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFV 120
               SSSSS+A+A+ AMVHK AG+  LWRQIHGCN+WE LVEP LHPLLRREVIRYGEFV
Sbjct: 61  IDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEFV 120

Query: 121 AACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPR 180
            ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP INIPPIQNSP 
Sbjct: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180

Query: 181 ACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDV 240
           +CGRWIGYVAVSSD  + RLGRRD+VITFRGTVT  EWIA LMSSLTPA LD +N+RPDV
Sbjct: 181 SCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPDV 240

Query: 241 KVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVL 300
           KV SGFL LYTC E  TKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGSAL +L
Sbjct: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALALL 300

Query: 301 LAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKM 360
           LAYDIAELGLNKRTNKE VP+SVFSFGGP+VGN  FKQRCEELGVKVLR+VNVNDPITK+
Sbjct: 301 LAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITKI 360

Query: 361 PGVLFNSYEHVGVELVLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDDGYGSG 420
           PGV F  YEHVGVELVL+FFNMQNPS VHDLETYISLL+Y ++    ++   DDD + +G
Sbjct: 361 PGV-FGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKE----EHQKHDDDHHHNG 420

Query: 421 RQKIII---VINKAMDFLCNN 423
           R+K+I     INKAM+FL  N
Sbjct: 421 RRKVINGGDFINKAMEFLSFN 436

BLAST of Csor.00g251100 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 9.46e-223
Identity = 335/446 (75.11%), Postives = 366/446 (82.06%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSS---------------- 60
           MA TTLKLN   NH TFS+T R   HSFGQVSLP++SD+VSS+S                
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSSSSSSVLVPL 60

Query: 61  ----SSSSSSSSSSTAAASTAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIR 120
               SSSSSSSSSST+AA  AMVHK AG+  LWRQIHGCN+WE LVEP LHPLLRREVIR
Sbjct: 61  IDMFSSSSSSSSSSTSAA-VAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIR 120

Query: 121 YGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPI 180
           YGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP INIPPI
Sbjct: 121 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI 180

Query: 181 QNSPRACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYN 240
           QNSP +CGRWIGYVAVSSD  + RLGRRD+VITFRGTVT  EWIA LMSSLTPA LD +N
Sbjct: 181 QNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHN 240

Query: 241 YRPDVKVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGS 300
           +RPDVKV SGFL LYTC E  TKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGS
Sbjct: 241 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGS 300

Query: 301 ALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVND 360
           AL +LLAYDIAELGLNKRTNKE VP+SVFSFGGP+VGN  FKQRCEELGVKVLR+VNVND
Sbjct: 301 ALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVND 360

Query: 361 PITKMPGVLFNSYEHVGVELVLEFFNMQNPSWVHDLETYISLLKYQEDDDDSDNSDGDDD 420
           PITKMPGV F  YEHVGVELVL+FFNMQNPS VHDLETYISLL+Y +  ++    D DD 
Sbjct: 361 PITKMPGV-FGGYEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPK--EEHQEHDDDDH 420

Query: 421 GYGSGRQKIII---VINKAMDFLCNN 423
            + +GR+K I     INKAM+FL  N
Sbjct: 421 HHHNGRRKAINGGDFINKAMEFLSFN 442

BLAST of Csor.00g251100 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 435.6 bits (1119), Expect = 4.5e-122
Identity = 245/460 (53.26%), Postives = 302/460 (65.65%), Query Frame = 0

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSS---SSSTAA 60
           M H     N  P    F     S P S+     PKR    +SSSSSS  +    +S  A+
Sbjct: 9   MKHAPFLRNRSPQQTLFIPYTLSLPISYQN---PKRLKTANSSSSSSLLAPVILNSPVAS 68

Query: 61  ASTAMVH-----------KNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVA 120
           +S   ++               ++ +WR+I GCNNW+DL+EP L+PLL++E+ RYG  V+
Sbjct: 69  SSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLVS 128

Query: 121 ACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYIYATPPF-INIPPIQNSP 180
            CYKAFDLDPNSKRYL CK+GK+ L KE  ++    Y+V+KYIYATP   INI PIQN  
Sbjct: 129 TCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEM 188

Query: 181 RACGRWIGYVAVSSDYMTTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPD 240
               RW+GYVA SSD    RLGRRDIV+TFRGTVT PEW+A  MSSLTPA    +N R D
Sbjct: 189 NRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLD 248

Query: 241 VKVLSGFLMLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGV 300
           VKV SGFL LYT +E  +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS+L  
Sbjct: 249 VKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQ 308

Query: 301 LLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELGLN+R  K  +P++VFSF GP+VGNL FK+RCEELGVKVLRI NVNDP+TK
Sbjct: 309 LLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTK 368

Query: 361 MPGVLFNS-----------------YEHVGVELVLEFFNMQNPSWVHDLETYISLLKYQE 420
           +PGVLFN                  Y HVGVEL L+FF++QN S VHDL+TYI LL  + 
Sbjct: 369 LPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLLNQRR 428

Query: 421 DDDDSDNSDGDDDGYGSGRQKIIIVINKAMDFLCNNAQNL 428
            +  S +SD D+D             N A++FL  N + +
Sbjct: 429 TNSRSVDSDEDEDS-----------DNVALEFLKTNGEKM 453

BLAST of Csor.00g251100 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 428.7 bits (1101), Expect = 5.5e-120
Identity = 233/390 (59.74%), Postives = 286/390 (73.33%), Query Frame = 0

Query: 13  NHFTFSD-TIRSNPHSFGQVSLPKRSDNVSSSSSSSSSSSSSSTAAASTAMVHKNAGMTS 72
           +HF+ S  T +    S   +S P  S  +S  SSSSS +   S A A T        ++ 
Sbjct: 29  DHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAVT------LPLSR 88

Query: 73  LWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNL 132
           +WR+I G NNWE+L+EP L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKKNL
Sbjct: 89  VWREIQGSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNL 148

Query: 133 FKEVGLEN-SGYEVSKYIYATPPFINIPPIQNSPRACGRWIGYVAVSSDYMTTRLGRRDI 192
            KE G+ +  GY+V+KYIYATP  IN+ PI+N P    RWIGYVAVSSD    RLGRRDI
Sbjct: 149 LKESGIHDPDGYQVTKYIYATPD-INLNPIKNEPNR-ARWIGYVAVSSDESVKRLGRRDI 208

Query: 193 VITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEKCTKFGLESCR 252
           ++TFRGTVT  EW+A L SSLTPA LD +N RPDVKV SGFL LYT  E  +KFGLESCR
Sbjct: 209 LVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCR 268

Query: 253 EQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFS 312
           EQLLSE+SRL+NK+K EE+SIT+AGHSMGS+L  LLAYDIAELG+N+R +++ VP++VFS
Sbjct: 269 EQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFS 328

Query: 313 FGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS-----------------Y 372
           F GP+VGNL FK+RCEELGVKVLRI NVNDPITK+PG LFN                  Y
Sbjct: 329 FAGPRVGNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCY 388

Query: 373 EHVGVELVLEFFNMQNPSWVHDLETYISLL 384
            HVGVEL L+FF++QN S VHDLETYI+L+
Sbjct: 389 THVGVELTLDFFDVQNISCVHDLETYITLV 409

BLAST of Csor.00g251100 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.3e-73
Identity = 164/388 (42.27%), Postives = 226/388 (58.25%), Query Frame = 0

Query: 73  WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLF 132
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +   F
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 133 KEVGLENSGYEVSKYIYATPPFINIPPIQNSPR------ACGRWIGYVAVSSDYMT-TRL 192
             +G+ +SGYEV++Y+YAT   IN+P   +  R          W+GYVAVS D  +  RL
Sbjct: 165 DSLGMIDSGYEVARYLYATSN-INLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 193 GRRDIVITFRGTVTIPEWIAILMSSLTPA---HLDCYNYRPDVKVLSGFLMLYTCNEKCT 252
           GRRDI I +RGTVT  EWIA L   L P     + C +  P VKV SGFL LYT  +   
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPD--PAVKVESGFLDLYTDKDTTC 284

Query: 253 KFGLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALGVLLAYDIAELGLNKRT 312
           KF   S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G AL +L AYDIAE+ LN+  
Sbjct: 285 KFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSK 344

Query: 313 NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------- 372
             + +P++V ++GGP+VGN+ F++R EELGVKV+R+VNV+D + K PG+  N        
Sbjct: 345 KGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALM 404

Query: 373 ---------YEHVGVELVLEFFNMQ------NPSWVHDLETYISLLKYQEDDDDSDNSDG 426
                    Y HVG EL L+  N        + S  H+LE  + LL       D  +  G
Sbjct: 405 KIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL-------DGYHGKG 464

BLAST of Csor.00g251100 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-71
Identity = 152/346 (43.93%), Postives = 209/346 (60.40%), Query Frame = 0

Query: 73  WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLF 132
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +   F
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 133 KEVGLENSGYEVSKYIYATPPFINIPPIQNSPR------ACGRWIGYVAVSSDYMT-TRL 192
             +G+ +SGYEV++Y+YAT   IN+P   +  R          W+GYVAVS D  +  RL
Sbjct: 165 DSLGMIDSGYEVARYLYATSN-INLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 193 GRRDIVITFRGTVTIPEWIAILMSSLTPA---HLDCYNYRPDVKVLSGFLMLYTCNEKCT 252
           GRRDI I +RGTVT  EWIA L   L P     + C +  P VKV SGFL LYT  +   
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPD--PAVKVESGFLDLYTDKDTTC 284

Query: 253 KFGLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALGVLLAYDIAELGLNKRT 312
           KF   S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G AL +L AYDIAE+ LN+  
Sbjct: 285 KFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSK 344

Query: 313 NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------- 372
             + +P++V ++GGP+VGN+ F++R EELGVKV+R+VNV+D + K PG+  N        
Sbjct: 345 KGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALM 404

Query: 373 ---------YEHVGVELVLEFFNMQ------NPSWVHDLETYISLL 384
                    Y HVG EL L+  N        + S  H+LE  + LL
Sbjct: 405 KIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 446

BLAST of Csor.00g251100 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.8e-70
Identity = 168/434 (38.71%), Postives = 240/434 (55.30%), Query Frame = 0

Query: 39  NVSSSSSSSSSSSSSSTAAASTAMVH----------------KNAGMTSLWRQIHGCNNW 98
           ++  SSS +S SSS++    S    H                K   +  +WR++ GCNNW
Sbjct: 41  SIICSSSCTSISSSTTQQKQSNKQTHVSDNKREEKAEEEEEEKEVSLREIWREVQGCNNW 100

Query: 99  EDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLE-NSG 158
           E  ++P ++  LRRE+IRYGEF  ACY +FD DP+SK   +CK+   + F  + L  + G
Sbjct: 101 EGQLDP-MNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFLNLDLHLHKG 160

Query: 159 YEVSKYIYAT-----PPFINIPPIQNSPRACGRWIGYVAVSSD-YMTTRLGRRDIVITFR 218
           Y +++Y+YAT     P F     + +       W+G+VAV++D    +RLGRRDIVI +R
Sbjct: 161 YTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRLGRRDIVIAWR 220

Query: 219 GTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLMLYTCNEKCTKFGLESCREQLLS 278
           GTVT  EWI  L   L  A+   +   P +K+  GF  LYT  E   KF   S REQ+L+
Sbjct: 221 GTVTYLEWIYDLKDILCSAN---FGDDPSIKIELGFHDLYTKKEDSCKFSSFSAREQVLA 280

Query: 279 EVSRLLNKYKNEE----VSITMAGHSMGSALGVLLAYDIAELGLNK-RTNKEAVPISVFS 338
           EV RL+  Y  EE     SIT+ GHS+G++L ++ AYDIAEL LN    N   +PI+VFS
Sbjct: 281 EVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNYKIPITVFS 340

Query: 339 FGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN-----------------SY 398
           F GP+VGNL FK+RC+ELGVKVLR+VNV+D +  +PG+  N                 SY
Sbjct: 341 FSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEEKTSFPWSY 400

Query: 399 EHVGVELVLEFFN------MQNPSWVHDLETYISLLK-YQEDDDDSDNSDGDDDGYGSGR 421
            HVGVEL L+          ++    H+LE  + L+  Y   D++++        +    
Sbjct: 401 AHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKR------FCLVT 460