Csor.00g245630 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g245630
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionN(4)-(Beta-n-acetylglucosaminyl)-l-asparaginase, putative
LocationCsor_Chr16: 1011957 .. 1015234 (+)
RNA-Seq ExpressionCsor.00g245630
SyntenyCsor.00g245630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTCCTTGCCTCCAATAATCGGTTTCGAGCTTCCGCCTTGCTTCTCGTCTTGCTCTTCCAGGTACTTGATATCACGACCCAGCACCGCCTTTCTCTGTTCAATGCCATTTTCAATTTCCTTAGTTTAGTGCTCGAAGCAGCTTAATATCTAAAAGAGAATTTGAGAAGCACCCATTTCTTCACTGACCGAAATTTTGTAGCCCGATTCTTCAATTTTCCGTTCTAGCTGTTATGGGTTTTTGGTTGAATGCAGATCAGTCATCTATACCATTGATCGGGGAGCTTTCCCATCTCCGATGTTCGTTTAAGTTCTTCCCCTCTTCTATAAATTCTTGGTTACGGGGTTTCGCTTGCTTATTTTCTTCACTGAAATGTTACATTTCCATTATCTTGGTGAAGTTTATATCGATTTTTGAGTTGTTAGGTTACTGGACGTGTAGCTGGAGAGTCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTTGAAAGGGCGTGGAGTGCTGTTAATAATGGCTCCTCAGCAGTTGATGCTGTTGTAGAAGGATGTTCTGCCTGTGAAGAACTGAGATGTGATGGCACAGGTGGGCACTTTAATTTGATGTGGGTCTAAATATTTGTTCTCAATGTGGAGTTTATTGGCTCATTCATTTCTATGGCTTAGCTATTGTGTCTTGTTCTATCTTCTGTGAAAAAAAAAATTATTGAGCAGTTCACTGGGTGCTTTCTTTATATGAACAATATGGATTTGCCAAAAATTCTGAACAGTGATGCTATCTGGTTTTTGAAGTTGAGGTTGCTTTCTGACGTTGATGATAACATCACCTGTTGAGGATTGTTGGGAGAGGAGTTTTATGTCAGCTAATTAACGGGTTGTTCATGAGTTTATAAGTAAGAAATACATCTCTATTGATATGAGGCCTTTTGGGGAAACCAAAAGTAGTGCCATGAGAGCTAATACTCAAAGTGGACAATATCATACTATTATGGAGGTTCGTGATTCCTAACATGGTATCAAAGTCATGCCTAACTTAGCCATGTCAATAGAATCTTCAAATGTCGAACAAAAAAGTTGGGACCCTTGAAAGTGTAGTCAAAAGTGACTCAAGTGTCAAACAAAGGATGTACTTTTGAGGGCTCCAAAGAAAGAGTCGAGCCTCGATTAAGGGGAGGCTATTCAAGGGCTCCATAGGCTTCAGGGGAGGCTCTATGGTGTACTTTGTTCGAGGGGAGGATTGTTGAGAATTGTTGGAAGAGGAGTCCCACGTCGGCTAATTGAGGGGTTGATCATGAGTTTATAAACAAGGAATACATCTCCATCGGTATGAGACCTTTTGGGGAAACAAAAAACAAAGCCACGAGAGCTAATACTCAAAGTGGACAATATCATACTAGTTTAATGGAGAGGTGTGTGCATATGAAGTGCCTAGTTTAATGTAATTTTTTATGAATAGTTGGACCAGGTGGAAGTCCGGATGAAAATGGAGAAACAACCATTGATGCCATGGTTATGGATGGGGTAAGCCTTCTCTGGATATTCACTTCCTGTTGCTCATCTTTGTATTTACTTTATATTCACTAAGGATATGCATAGGTGACTATGGAAGTTGGAGCTGTAGCCGCCATGAGGTATATCAAAGATGGAATAAAAGCTGCAAGATTAGTGATGAGACATACCCAACACACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCATTTCAATGGGCCTTCCCGGACCCGAAGATCTTAGCTCGTCGGAGTCGATCGAAAAGTGGAACAAATGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTGTCAATAGCTGTGGCCCTTACCACTCTAATGACCTTATGCTTGTTGCTGAGACAACATGTTCAGGAGATGATCCAATGAGAACTGTTGGATTAAGGTCGAATCATTTTGGTCTTCACAGTCACGATACCATATCGATGGCAGTAATCGATAAGGTAAGTTGATAAGTGAAGTTAATTCTCTCTTGTCATTGTATTCATATTAAAGATTTGTTTCTTGAAATAGTTTGGGCACATTGCTGTTGGAACATCCACTAATGGAGCCACCTTCAAGATTCCAGGCAGGTAAGTTCTGTTAGATGCCTACTCTTTATTCCGCTCTATTACCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCTTCAAAGTTTTTAAAATGCGTTGGATAGGGAGAGGTTTCTACACCCTTATAAAGGGTGTTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAATCCACCCCCCTCCTTCGGGGTCCAGTGTCCTCGCTGGCAGTCATTCCCTTCTTCAATCGATGTGGGACCCCCAATCCCCTTCGGGGCTCAACGTCCTTGCTGGCACACCGCCTCGTGTTCACCCCCTTTGGGGCTCTGGAACATCGCCCGGTGTCTAGCTCTGATACCATTTGTAACAGCCCAAGCGCATAGCTAGCAGATATTGTCTTCTTTGGGCTTTCCCTTTTGGGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGTTAGGGAAAAGTTTCCATGTCCTTACAAAGGGTGTTTTGTTCCTCTCCAACCGATATGAGATCTCACATCTACCATCTATTGAAATCTCAGTCATTTTCTTTTGTTATTCCCTTCATTTGGTGGTTGGGTATTGCATAAGTAACCAGACGTCGCTGAAGCTTTTTGGATCACGCCTCTCTGAATCAACTTTCTTTGATGCAGGGTGGGTGATGGACCTATAGCAGGATCTTCAGCATATGCAGATAGTGATATCGGTGCATGTGGAGCAACCGGGGATGGCGACATCATGATGCGTTTCCTCCCGTGGTAAACAAACCATTTCTAGCCATTATTATGCAACTCTACTCTCTGAAACTCAACGCATAAGCTTTGATATGTTCCATGCAACGAACAGATTGATCCGTGTCTATTTGCTTGTTCAATAATTCTTCACTCTCTATCTGTCGCATCGTCCCGTTTTGGTTGAATCTCGAAAATACTTCTGCTTGATTACAGCTATCAAGTCGTCAAGAGTATGCGACTGGGGATGGAACCGAAGGACGCTGCGAAAGATGCAATATCAAGGATTGCAAGGAAGTTCCCTGACTTCATTGGAGCTATTTTTGCTGTTAACAAAAATGGTACTCATGCTGGTGCTTGCCATGGATGGACATTCCAATACTCGGTCCGAAGCCCAGAAATGCATCACGCCGAGGTTTTCACGGTACTTCCCTGA

mRNA sequence

ATGGAACTCCTTGCCTCCAATAATCGGTTTCGAGCTTCCGCCTTGCTTCTCGTCTTGCTCTTCCAGGTTACTGGACGTGTAGCTGGAGAGTCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTTGAAAGGGCGTGGAGTGCTGTTAATAATGGCTCCTCAGCAGTTGATGCTGTTGTAGAAGGATGTTCTGCCTGTGAAGAACTGAGATGTGATGGCACAGTTGGACCAGGTGGAAGTCCGGATGAAAATGGAGAAACAACCATTGATGCCATGGTTATGGATGGGGTGACTATGGAAGTTGGAGCTGTAGCCGCCATGAGGTATATCAAAGATGGAATAAAAGCTGCAAGATTAGTGATGAGACATACCCAACACACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCATTTCAATGGGCCTTCCCGGACCCGAAGATCTTAGCTCGTCGGAGTCGATCGAAAAGTGGAACAAATGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTGTCAATAGCTGTGGCCCTTACCACTCTAATGACCTTATGCTTGTTGCTGAGACAACATGTTCAGGAGATGATCCAATGAGAACTGTTGGATTAAGGTCGAATCATTTTGGTCTTCACAGTCACGATACCATATCGATGGCAGTAATCGATAAGTTTGGGCACATTGCTGTTGGAACATCCACTAATGGAGCCACCTTCAAGATTCCAGGCAGGGTGGGTGATGGACCTATAGCAGGATCTTCAGCATATGCAGATAGTGATATCGGTGCATGTGGAGCAACCGGGGATGGCGACATCATGATGCGTTTCCTCCCGTGCTATCAAGTCGTCAAGAGTATGCGACTGGGGATGGAACCGAAGGACGCTGCGAAAGATGCAATATCAAGGATTGCAAGGAAGTTCCCTGACTTCATTGGAGCTATTTTTGCTGTTAACAAAAATGGTACTCATGCTGGTGCTTGCCATGGATGGACATTCCAATACTCGGTCCGAAGCCCAGAAATGCATCACGCCGAGGTTTTCACGGTACTTCCCTGA

Coding sequence (CDS)

ATGGAACTCCTTGCCTCCAATAATCGGTTTCGAGCTTCCGCCTTGCTTCTCGTCTTGCTCTTCCAGGTTACTGGACGTGTAGCTGGAGAGTCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTTGAAAGGGCGTGGAGTGCTGTTAATAATGGCTCCTCAGCAGTTGATGCTGTTGTAGAAGGATGTTCTGCCTGTGAAGAACTGAGATGTGATGGCACAGTTGGACCAGGTGGAAGTCCGGATGAAAATGGAGAAACAACCATTGATGCCATGGTTATGGATGGGGTGACTATGGAAGTTGGAGCTGTAGCCGCCATGAGGTATATCAAAGATGGAATAAAAGCTGCAAGATTAGTGATGAGACATACCCAACACACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCATTTCAATGGGCCTTCCCGGACCCGAAGATCTTAGCTCGTCGGAGTCGATCGAAAAGTGGAACAAATGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTGTCAATAGCTGTGGCCCTTACCACTCTAATGACCTTATGCTTGTTGCTGAGACAACATGTTCAGGAGATGATCCAATGAGAACTGTTGGATTAAGGTCGAATCATTTTGGTCTTCACAGTCACGATACCATATCGATGGCAGTAATCGATAAGTTTGGGCACATTGCTGTTGGAACATCCACTAATGGAGCCACCTTCAAGATTCCAGGCAGGGTGGGTGATGGACCTATAGCAGGATCTTCAGCATATGCAGATAGTGATATCGGTGCATGTGGAGCAACCGGGGATGGCGACATCATGATGCGTTTCCTCCCGTGCTATCAAGTCGTCAAGAGTATGCGACTGGGGATGGAACCGAAGGACGCTGCGAAAGATGCAATATCAAGGATTGCAAGGAAGTTCCCTGACTTCATTGGAGCTATTTTTGCTGTTAACAAAAATGGTACTCATGCTGGTGCTTGCCATGGATGGACATTCCAATACTCGGTCCGAAGCCCAGAAATGCATCACGCCGAGGTTTTCACGGTACTTCCCTGA

Protein sequence

MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP
Homology
BLAST of Csor.00g245630 vs. ExPASy Swiss-Prot
Match: Q56W64 (Probable isoaspartyl peptidase/L-asparaginase 3 OS=Arabidopsis thaliana OX=3702 GN=At5g61540 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 3.7e-143
Identity = 249/351 (70.94%), Postives = 291/351 (82.91%), Query Frame = 0

Query: 10  FRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCS 69
           F ++ LL + L  V      +S  +P+VVSTWPFLEAV  AW AV+NGSSAV+AVVEGCS
Sbjct: 9   FVSTLLLFLSLLTVADAELVKSDKFPVVVSTWPFLEAVRAAWRAVDNGSSAVEAVVEGCS 68

Query: 70  ACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHT 129
           ACEELRCDGTVGPGGSPDENGET IDA+VMDGVTMEVGAVAAMRY+KDGI+AA LVM+++
Sbjct: 69  ACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYS 128

Query: 130 QHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCGPYH 189
           QHTLL GE ASAF+ISMGLPGP +LSS ES++KW+ WKEN CQPNF KNVVP N CGPY 
Sbjct: 129 QHTLLAGEGASAFAISMGLPGPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYK 188

Query: 190 -SNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFK 249
            +N  M V     +    M  +  +    G H+HDTISMAVID+ GHIAVGTSTNGAT+K
Sbjct: 189 PNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATYK 248

Query: 250 IPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAI 309
           IPGRVGDGPI GSSAYAD ++G CGATGDGD MMRFLPCYQVV+SMR GM+P++AAKDAI
Sbjct: 249 IPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMRQGMKPEEAAKDAI 308

Query: 310 SRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 360
           SRIARKFPDF+GA+ AV+KNG+HAGAC+GWTFQYSV++P+M+  +VFTVLP
Sbjct: 309 SRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQYSVQNPDMNDVQVFTVLP 359

BLAST of Csor.00g245630 vs. ExPASy Swiss-Prot
Match: Q64191 (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Mus musculus OX=10090 GN=Aga PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.3e-83
Identity = 164/329 (49.85%), Postives = 208/329 (63.22%), Query Frame = 0

Query: 11  RASALLLVLLFQVTGR-VAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCS 70
           R S L L+LL  V G  +   S   PLVV+TWPF  A E AW  + +G SA+DAV  GC+
Sbjct: 3   RKSNLSLLLLLLVLGMPLVRGSSPLPLVVNTWPFKNATEAAWWTLLSGGSALDAVENGCA 62

Query: 71  ACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHT 130
            CE+ +CDGTVG GGSPDE GETT+DAM+MDG  M+VGAV  +R IK+ I  AR V+ HT
Sbjct: 63  VCEKEQCDGTVGFGGSPDEGGETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARRVLEHT 122

Query: 131 QHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNS--CGP 190
            HTLLVG+ A+ F+ SMG    EDLS+  S +  + W   NCQPN+W+NV+P  S  CGP
Sbjct: 123 THTLLVGDSATKFAESMGFTN-EDLSTKTSRDLHSDWLSRNCQPNYWRNVIPDPSKYCGP 182

Query: 191 YHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATF 250
           Y  +  +             +++        +HSHDTI M VI K GH A GTSTNG  F
Sbjct: 183 YKPSGFL------------KQSISPHKEEVDIHSHDTIGMVVIHKTGHTAAGTSTNGIKF 242

Query: 251 KIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDA 310
           KIPGRVGD PI G+ AYAD   GA  ATGDGD ++RFLP YQ V+ MR G +P  A +  
Sbjct: 243 KIPGRVGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRGGDDPAIACQKV 302

Query: 311 ISRIARKFPDFIGAIFAVNKNGTHAGACH 337
           I RI + +P+F GA+   + NG++  AC+
Sbjct: 303 ILRIQKYYPNFFGAVICASVNGSYGAACN 318

BLAST of Csor.00g245630 vs. ExPASy Swiss-Prot
Match: P20933 (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Homo sapiens OX=9606 GN=AGA PE=1 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 4.1e-81
Identity = 164/313 (52.40%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 31  SGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCSACEELRCDGTVGPGGSPDENG 90
           S   PLVV+TWPF  A E AW A+ +G SA+DAV  GC+ CE  +CDG+VG GGSPDE G
Sbjct: 24  SSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELG 83

Query: 91  ETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPG 150
           ETT+DAM+MDG TM+VGAV  +R IK+ I  AR V+ HT HTLLVGE A+ F+ SMG   
Sbjct: 84  ETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFIN 143

Query: 151 PEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNS--CGPYH-----SNDLMLVAETTCSG 210
            EDLS++ S    + W   NCQPN+W+NV+P  S  CGPY        D+ +  ET    
Sbjct: 144 -EDLSTTASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETE--- 203

Query: 211 DDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSA 270
           DD                HDTI M VI K GHIA GTSTNG  FKI GRVGD PI G+ A
Sbjct: 204 DD--------------RGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGA 263

Query: 271 YADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIF 330
           YAD   GA  ATG+GDI+MRFLP YQ V+ MR G +P  A +  ISRI + FP+F GA+ 
Sbjct: 264 YADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVI 318

Query: 331 AVNKNGTHAGACH 337
             N  G++  AC+
Sbjct: 324 CANVTGSYGAACN 318

BLAST of Csor.00g245630 vs. ExPASy Swiss-Prot
Match: P30919 (N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase OS=Rattus norvegicus OX=10116 GN=Aga PE=1 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 5.3e-81
Identity = 157/308 (50.97%), Postives = 195/308 (63.31%), Query Frame = 0

Query: 31  SGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCSACEELRCDGTVGPGGSPDENG 90
           S   PLVV+TWPF  A E AW  + +G SA+DAV +GC+ CE+ +C GTVG GGSPDE G
Sbjct: 24  SNPLPLVVNTWPFKNATEAAWWTLVSGGSALDAVEKGCAMCEKEQCGGTVGFGGSPDEVG 83

Query: 91  ETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPG 150
           ETT+DAM+MDG  M+VGAV  +R IK+ I  AR V+ HT HTLLVG+ A+ F++SMG   
Sbjct: 84  ETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARKVLEHTTHTLLVGDSATKFAVSMGFTS 143

Query: 151 PEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNS--CGPYHSNDLMLVAETTCSGDDPMR 210
            EDLS++ S    + W   NCQPN+W+NV+P  S  CGPY   D              + 
Sbjct: 144 -EDLSTNTSRALHSDWLSRNCQPNYWRNVIPDPSKYCGPYKPPDF-------------LE 203

Query: 211 TVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSD 270
                     +HSHDTI M VI K GH A GTSTNG  FKIPGRVGD PI G+ AYAD  
Sbjct: 204 QNNRAHKEVDIHSHDTIGMVVIHKTGHTAAGTSTNGLKFKIPGRVGDSPIPGAGAYADDM 263

Query: 271 IGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIFAVNKN 330
            GA  ATGDGD ++RFLP YQ V+ MR G +P  A +  ISRI + +P F GA+   N  
Sbjct: 264 AGAAAATGDGDTLLRFLPSYQAVEYMRGGDDPARACQKVISRIQKYYPKFFGAVICANVT 317

Query: 331 GTHAGACH 337
           G++  AC+
Sbjct: 324 GSYGAACN 317

BLAST of Csor.00g245630 vs. ExPASy Swiss-Prot
Match: B4NWI1 (Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase GE19290 OS=Drosophila yakuba OX=7245 GN=GE19290 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 9.1e-81
Identity = 167/320 (52.19%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 35  PLVVSTWPFLEAVERAWSAVNNGSSAV----DAVVEGCSACEELRCDGTVGPGGSPDENG 94
           P+V++TW F  A   AW  +      +    +AVVEGCS CE+L+CD TVG GGSPDE G
Sbjct: 60  PMVINTWNFTAANVLAWRILKQSKGGLRQTRNAVVEGCSKCEKLQCDRTVGYGGSPDELG 119

Query: 95  ETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPG 154
           ETT+DAMVMDG TMEVGAVA +R IKD IK AR V+ HTQHT+LVG+ ASAF+ +MG   
Sbjct: 120 ETTLDAMVMDGATMEVGAVAGLRRIKDAIKVARHVLEHTQHTMLVGDAASAFANAMGFES 179

Query: 155 PEDLSSSESIEKWNKWKENNCQPNFWKNV--VPVNSCGPYHSNDLMLVAETTCSGDDPMR 214
            E L + ES + W +W   NCQPNFWKNV   P  SCGPY      L    T   +D  R
Sbjct: 180 -ESLVTPESKDMWLQWTAENCQPNFWKNVHPDPKVSCGPYKPRPTPL----TRWKEDRAR 239

Query: 215 TVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSD 274
                    G  +HDTI M  ID   +I  GTSTNGA  KIPGRVGD PI G+ AYAD++
Sbjct: 240 ----NEYEIGRKNHDTIGMIAIDVESNIHAGTSTNGARHKIPGRVGDSPIPGAGAYADNE 299

Query: 275 IGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIFAVNKN 334
           +GA  ATGDGD+MMRFLP    V++MR G  P DAA++++ RI R   DF+GA+ AV++ 
Sbjct: 300 VGAAVATGDGDVMMRFLPSLLAVEAMRAGKPPADAAEESLRRIIRHHKDFMGALIAVDRL 359

Query: 335 GTHAGACHGW-TFQYSVRSP 348
           G +  AC+G   F + V SP
Sbjct: 360 GRYGAACYGLDEFPFMVSSP 370

BLAST of Csor.00g245630 vs. NCBI nr
Match: KAG6576819.1 (putative isoaspartyl peptidase/L-asparaginase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 728 bits (1878), Expect = 7.47e-265
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60
           MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA
Sbjct: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60

Query: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120
           VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK
Sbjct: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120

Query: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180
           AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV
Sbjct: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180

Query: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240
           PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT
Sbjct: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240

Query: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP 300
           STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP
Sbjct: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP 300

Query: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP
Sbjct: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

BLAST of Csor.00g245630 vs. NCBI nr
Match: XP_022922742.1 (probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita moschata])

HSP 1 Score: 724 bits (1870), Expect = 1.24e-263
Identity = 357/359 (99.44%), Postives = 358/359 (99.72%), Query Frame = 0

Query: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60
           MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPF EAVERAWSAVNNGSSA
Sbjct: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFFEAVERAWSAVNNGSSA 60

Query: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120
           VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK
Sbjct: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120

Query: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180
           AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV
Sbjct: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180

Query: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240
           PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT
Sbjct: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240

Query: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP 300
           STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGMEP
Sbjct: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMEP 300

Query: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP
Sbjct: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

BLAST of Csor.00g245630 vs. NCBI nr
Match: XP_023552142.1 (probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724 bits (1870), Expect = 1.24e-263
Identity = 357/359 (99.44%), Postives = 358/359 (99.72%), Query Frame = 0

Query: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60
           MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA
Sbjct: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60

Query: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120
           VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK
Sbjct: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120

Query: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180
           AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV
Sbjct: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180

Query: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240
           PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFG HSHDTISMAVIDKFGHIAVGT
Sbjct: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGFHSHDTISMAVIDKFGHIAVGT 240

Query: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP 300
           STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGMEP
Sbjct: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMEP 300

Query: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP
Sbjct: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

BLAST of Csor.00g245630 vs. NCBI nr
Match: XP_022985489.1 (probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita maxima])

HSP 1 Score: 712 bits (1837), Expect = 1.43e-258
Identity = 352/361 (97.51%), Postives = 358/361 (99.17%), Query Frame = 0

Query: 1   MELLA--SNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60
           MEL A  SNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS
Sbjct: 1   MELYAHDSNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120
           SAVDAVV+GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVDGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180
           IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240
           VVPVNSCGPY+SNDLM+VAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPVNSCGPYYSNDLMVVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVL 359
           +PKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTF+YSVRSPEMHHAEVFTVL
Sbjct: 301 DPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFKYSVRSPEMHHAEVFTVL 360

BLAST of Csor.00g245630 vs. NCBI nr
Match: XP_038900285.1 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 672 bits (1735), Expect = 5.05e-243
Identity = 332/361 (91.97%), Postives = 344/361 (95.29%), Query Frame = 0

Query: 1   MELLA--SNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60
           MEL A  S+NR RASALLL+L FQ+TGRVAGESG YPLVVSTWPFLEAVERAWSAVNNG 
Sbjct: 1   MELFALDSDNRLRASALLLLLFFQMTGRVAGESGLYPLVVSTWPFLEAVERAWSAVNNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120
           SAVD+VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG
Sbjct: 61  SAVDSVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180
           IKAARLVMRHT+HTLLVGEKASAFSISMGLPGP DLSS ESIEKWNKWKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTKHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240
           VVPVNSCGPYHSN L+LVAETTC GDDP R V LRSNHFGLHSHDTISMAVIDKFG+IAV
Sbjct: 181 VVPVNSCGPYHSNGLLLVAETTCPGDDPRRAVELRSNHFGLHSHDTISMAVIDKFGYIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVL 359
           +PKDAAKDAISRIARKFPDF+GAIFAVNKNGTHAGACHGWTFQYSVRSPEM+  E+FTV 
Sbjct: 301 KPKDAAKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMYRTEIFTVH 360

BLAST of Csor.00g245630 vs. ExPASy TrEMBL
Match: A0A6J1E462 (probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430645 PE=4 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 6.00e-264
Identity = 357/359 (99.44%), Postives = 358/359 (99.72%), Query Frame = 0

Query: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSA 60
           MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPF EAVERAWSAVNNGSSA
Sbjct: 1   MELLASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFFEAVERAWSAVNNGSSA 60

Query: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120
           VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK
Sbjct: 61  VDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIK 120

Query: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180
           AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV
Sbjct: 121 AARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVV 180

Query: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240
           PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT
Sbjct: 181 PVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGT 240

Query: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEP 300
           STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGMEP
Sbjct: 241 STNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMEP 300

Query: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP
Sbjct: 301 KDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

BLAST of Csor.00g245630 vs. ExPASy TrEMBL
Match: A0A6J1JDF2 (probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita maxima OX=3661 GN=LOC111483481 PE=4 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 6.94e-259
Identity = 352/361 (97.51%), Postives = 358/361 (99.17%), Query Frame = 0

Query: 1   MELLA--SNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60
           MEL A  SNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS
Sbjct: 1   MELYAHDSNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120
           SAVDAVV+GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVDGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180
           IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240
           VVPVNSCGPY+SNDLM+VAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPVNSCGPYYSNDLMVVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVL 359
           +PKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTF+YSVRSPEMHHAEVFTVL
Sbjct: 301 DPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFKYSVRSPEMHHAEVFTVL 360

BLAST of Csor.00g245630 vs. ExPASy TrEMBL
Match: A0A1S3BWS8 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494471 PE=4 SV=1)

HSP 1 Score: 661 bits (1706), Expect = 6.42e-239
Identity = 326/356 (91.57%), Postives = 333/356 (93.54%), Query Frame = 0

Query: 4   LASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDA 63
           L S +R RASALLL+LLFQVT RVAG S  YPLVVSTWPFLEAVERAWSA NNG SAVD+
Sbjct: 6   LDSKDRLRASALLLILLFQVTERVAGASSLYPLVVSTWPFLEAVERAWSAANNGYSAVDS 65

Query: 64  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 123
           VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR
Sbjct: 66  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 125

Query: 124 LVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVN 183
           LVMRHT HTLLVGEKASAFSISMGLPGP DLSS ESIEKWNKWKENNCQPNFWKNVVP N
Sbjct: 126 LVMRHTGHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKNVVPAN 185

Query: 184 SCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN 243
           SCGPYHSN L+L AETTC GD P R V LRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN
Sbjct: 186 SCGPYHSNGLLLAAETTCLGDGPRRAVELRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN 245

Query: 244 GATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDA 303
           GATFKIPGRVGDGPIAGSSAY DSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM PKDA
Sbjct: 246 GATFKIPGRVGDGPIAGSSAYVDSDIGACGATGDGDIMMRFLPCYQVVESMRLGMTPKDA 305

Query: 304 AKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           AKDAISRIARKFPDF+GAIFAVNKNGTHAGACHGWTFQYSVRSPEMH A+VFTVLP
Sbjct: 306 AKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHSAKVFTVLP 361

BLAST of Csor.00g245630 vs. ExPASy TrEMBL
Match: A0A5A7TRA8 (Putative isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004220 PE=4 SV=1)

HSP 1 Score: 661 bits (1705), Expect = 9.12e-239
Identity = 326/356 (91.57%), Postives = 333/356 (93.54%), Query Frame = 0

Query: 4   LASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDA 63
           L S +R RASALLL+LLFQVT RVAG S  YPLVVSTWPFLEAVERAWSA NNG SAVD+
Sbjct: 6   LDSKDRLRASALLLILLFQVTERVAGASSLYPLVVSTWPFLEAVERAWSAANNGYSAVDS 65

Query: 64  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 123
           VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR
Sbjct: 66  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 125

Query: 124 LVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVN 183
           LVMRHT HTLLVGEKASAFSISMGLPGP DLSS ESIEKWNKWKENNCQPNFWKNVVP N
Sbjct: 126 LVMRHTGHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKNVVPAN 185

Query: 184 SCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN 243
           SCGPYHSN L+L AETTC GD P R V LRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN
Sbjct: 186 SCGPYHSNGLLLAAETTCLGDGPRRAVELRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN 245

Query: 244 GATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDA 303
           GATFKIPGRVGDGPIAGSSAY DSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM PKDA
Sbjct: 246 GATFKIPGRVGDGPIAGSSAYVDSDIGACGATGDGDIMMRFLPCYQVVESMRLGMMPKDA 305

Query: 304 AKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           AKDAISRIARKFPDF+GAIFAVNKNGTHAGACHGWTFQYSVRSPEMH A+VFTVLP
Sbjct: 306 AKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHSAKVFTVLP 361

BLAST of Csor.00g245630 vs. ExPASy TrEMBL
Match: A0A0A0KWA2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022950 PE=4 SV=1)

HSP 1 Score: 657 bits (1694), Expect = 4.32e-237
Identity = 325/356 (91.29%), Postives = 335/356 (94.10%), Query Frame = 0

Query: 4   LASNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDA 63
           L S +R RASALLL+LLF VT RVAGES  YPLVVSTWPFLEAVERAWSA NNG+SAVD+
Sbjct: 6   LDSKDRLRASALLLILLFLVTERVAGESSLYPLVVSTWPFLEAVERAWSAANNGNSAVDS 65

Query: 64  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 123
           VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR
Sbjct: 66  VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAAR 125

Query: 124 LVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVN 183
           LVMRHT+HTLLVGEKASAFSISMGLPGP DLSS ESIEKWNKWKENNCQPNF KNVVPVN
Sbjct: 126 LVMRHTEHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFRKNVVPVN 185

Query: 184 SCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTN 243
           SCGPYHSN L+LVAETTC GD P R V LRSNHFG HSHDTISMAVIDKFGHIAVGTSTN
Sbjct: 186 SCGPYHSNGLLLVAETTCLGDGPRRAVELRSNHFGRHSHDTISMAVIDKFGHIAVGTSTN 245

Query: 244 GATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDA 303
           GATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVV+SMRLGM PKDA
Sbjct: 246 GATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMTPKDA 305

Query: 304 AKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359
           AKDAI+RIARKFPDF+GAIFAV+KNGTHAGACHGW FQYSVRSPEMH AEVFTVLP
Sbjct: 306 AKDAIARIARKFPDFVGAIFAVDKNGTHAGACHGWKFQYSVRSPEMHSAEVFTVLP 361

BLAST of Csor.00g245630 vs. TAIR 10
Match: AT5G61540.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 509.2 bits (1310), Expect = 2.7e-144
Identity = 249/351 (70.94%), Postives = 291/351 (82.91%), Query Frame = 0

Query: 10  FRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVEGCS 69
           F ++ LL + L  V      +S  +P+VVSTWPFLEAV  AW AV+NGSSAV+AVVEGCS
Sbjct: 9   FVSTLLLFLSLLTVADAELVKSDKFPVVVSTWPFLEAVRAAWRAVDNGSSAVEAVVEGCS 68

Query: 70  ACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVMRHT 129
           ACEELRCDGTVGPGGSPDENGET IDA+VMDGVTMEVGAVAAMRY+KDGI+AA LVM+++
Sbjct: 69  ACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYS 128

Query: 130 QHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCGPYH 189
           QHTLL GE ASAF+ISMGLPGP +LSS ES++KW+ WKEN CQPNF KNVVP N CGPY 
Sbjct: 129 QHTLLAGEGASAFAISMGLPGPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYK 188

Query: 190 -SNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGATFK 249
            +N  M V     +    M  +  +    G H+HDTISMAVID+ GHIAVGTSTNGAT+K
Sbjct: 189 PNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATYK 248

Query: 250 IPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKDAI 309
           IPGRVGDGPI GSSAYAD ++G CGATGDGD MMRFLPCYQVV+SMR GM+P++AAKDAI
Sbjct: 249 IPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMRQGMKPEEAAKDAI 308

Query: 310 SRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 360
           SRIARKFPDF+GA+ AV+KNG+HAGAC+GWTFQYSV++P+M+  +VFTVLP
Sbjct: 309 SRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQYSVQNPDMNDVQVFTVLP 359

BLAST of Csor.00g245630 vs. TAIR 10
Match: AT5G61540.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.2e-105
Identity = 182/257 (70.82%), Postives = 215/257 (83.66%), Query Frame = 0

Query: 104 MEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKW 163
           MEVGAVAAMRY+KDGI+AA LVM+++QHTLL GE ASAF+ISMGLPGP +LSS ES++KW
Sbjct: 1   MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 60

Query: 164 NKWKENNCQPNFWKNVVPVNSCGPYH-SNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSH 223
           + WKEN CQPNF KNVVP N CGPY  +N  M V     +    M  +  +    G H+H
Sbjct: 61  SDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNH 120

Query: 224 DTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMM 283
           DTISMAVID+ GHIAVGTSTNGAT+KIPGRVGDGPI GSSAYAD ++G CGATGDGD MM
Sbjct: 121 DTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMM 180

Query: 284 RFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQY 343
           RFLPCYQVV+SMR GM+P++AAKDAISRIARKFPDF+GA+ AV+KNG+HAGAC+GWTFQY
Sbjct: 181 RFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQY 240

Query: 344 SVRSPEMHHAEVFTVLP 360
           SV++P+M+  +VFTVLP
Sbjct: 241 SVQNPDMNDVQVFTVLP 257

BLAST of Csor.00g245630 vs. TAIR 10
Match: AT5G61540.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.2e-105
Identity = 182/257 (70.82%), Postives = 215/257 (83.66%), Query Frame = 0

Query: 104 MEVGAVAAMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKW 163
           MEVGAVAAMRY+KDGI+AA LVM+++QHTLL GE ASAF+ISMGLPGP +LSS ES++KW
Sbjct: 1   MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 60

Query: 164 NKWKENNCQPNFWKNVVPVNSCGPYH-SNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSH 223
           + WKEN CQPNF KNVVP N CGPY  +N  M V     +    M  +  +    G H+H
Sbjct: 61  SDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNH 120

Query: 224 DTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMM 283
           DTISMAVID+ GHIAVGTSTNGAT+KIPGRVGDGPI GSSAYAD ++G CGATGDGD MM
Sbjct: 121 DTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMM 180

Query: 284 RFLPCYQVVKSMRLGMEPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQY 343
           RFLPCYQVV+SMR GM+P++AAKDAISRIARKFPDF+GA+ AV+KNG+HAGAC+GWTFQY
Sbjct: 181 RFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQY 240

Query: 344 SVRSPEMHHAEVFTVLP 360
           SV++P+M+  +VFTVLP
Sbjct: 241 SVQNPDMNDVQVFTVLP 257

BLAST of Csor.00g245630 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 8.9e-15
Identity = 81/286 (28.32%), Postives = 123/286 (43.01%), Query Frame = 0

Query: 53  AVNNGSSAVDAVVEGCSACEELRCDG--TVGPGGSPDENGETTIDAMVMDGVTMEVGAVA 112
           A+ +  SA+D VVE      EL  D     G G +  E G   ++A +MDG     GAV+
Sbjct: 40  ALRSNVSAID-VVE--LVIRELETDPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVS 99

Query: 113 AMRYIKDGIKAARLVMRHTQHTLLVGEKASAFSISMG--LPGPEDLSSSESIEKWNKWKE 172
            +  +K+ I  ARLVM  + H+ L    A  F+   G  +   E   + +++      KE
Sbjct: 100 GITTVKNPISLARLVMDKSPHSYLAFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKE 159

Query: 173 NNCQPNFWKNVVPVNSCGPYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMA 232
            N    F   + P+   G               + D P++  GL      +++ +T+   
Sbjct: 160 AN-SILFDYRIPPMGCAG-------------AAATDSPIQMNGL---PISIYAPETVGCV 219

Query: 233 VIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCY 292
           V+D  GH A GTST G   K+ GR+GD P+ G+  YA S+      TG+G+ ++R     
Sbjct: 220 VVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYA-SEFCGVSCTGEGEAIIRATLAR 279

Query: 293 QVVKSMRL-GMEPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAG 334
            V   M   G+  ++A    I    R    F G I   NK     G
Sbjct: 280 DVSAVMEYKGLNLQEAVDYVIKH--RLDEGFAGLIAVSNKGEVVCG 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56W643.7e-14370.94Probable isoaspartyl peptidase/L-asparaginase 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q641913.3e-8349.85N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Mus musculus OX=10090 GN=Aga ... [more]
P209334.1e-8152.40N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Homo sapiens OX=9606 GN=AGA P... [more]
P309195.3e-8150.97N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase OS=Rattus norvegicus OX=10116 GN... [more]
B4NWI19.1e-8152.19Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase GE19290 OS=Drosophila y... [more]
Match NameE-valueIdentityDescription
KAG6576819.17.47e-265100.00putative isoaspartyl peptidase/L-asparaginase 3, partial [Cucurbita argyrosperma... [more]
XP_022922742.11.24e-26399.44probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita moschata][more]
XP_023552142.11.24e-26399.44probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita pepo subsp. pepo][more]
XP_022985489.11.43e-25897.51probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita maxima][more]
XP_038900285.15.05e-24391.97probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1E4626.00e-26499.44probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JDF26.94e-25997.51probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A1S3BWS86.42e-23991.57probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo OX=36... [more]
A0A5A7TRA89.12e-23991.57Putative isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo var. ... [more]
A0A0A0KWA24.32e-23791.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022950 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G61540.12.7e-14470.94N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G61540.24.2e-10570.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G61540.34.2e-10570.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.18.9e-1528.32N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 44..340
e-value: 3.0E-76
score: 256.4
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 17..358
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 224..357
e-value: 1.8E-40
score: 140.3
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 32..336
e-value: 2.8E-82
score: 274.2
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 32..336
e-value: 2.8E-82
score: 274.2
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 32..336
e-value: 2.8E-82
score: 274.2
NoneNo IPR availablePANTHERPTHR10188:SF6N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASEcoord: 17..358
NoneNo IPR availableCDDcd04513Glycosylasparaginasecoord: 36..346
e-value: 6.16744E-172
score: 478.209
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 34..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g245630.m01Csor.00g245630.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006517 protein deglycosylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003948 N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity
molecular_function GO:0016787 hydrolase activity