Csor.00g245380 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g245380
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr16: 1136895 .. 1138745 (+)
RNA-Seq ExpressionCsor.00g245380
SyntenyCsor.00g245380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTATTTTTGTGTCGGGTTTTATCTTTTTCACTGTCATTTTAAGTTCGTGGACTGTATTAGCTTCATCTTCTTTCAATGTTCTCGATTATGGAGCTGCTGGAAATGGCGAAACTGATGATACAGAAGTATGTAATTTAAACTCGATTTTTTATTTCCATGCAAAAAATTGATTGATTCGCTCTCTAATTTTTCTCTTTGTTATAAGGCTTTTGTGAAAGCTTGGAAGGATGTCTGTGAAGCAACTGATGACATTCCAACATTGCAAGTACCTGCTACAAAAATTTACCTTTTGAACCCTATCACATTTGAAGGTCCTTGCAAGTCTGAACAAGTCAATTTTGAGGTAATTTATCTTACCTATATATATATATATATAGTCTGAACAAGTCAATTTGGAGGTAATTTATCTTACTCATATATATATAATTTTTTTTGTCACATTTTCTTTTATCATATGATTTTTGAACGTATTGTGAATTTGCAGCTTAAAGGAACTCTAATGGCTCCGAGCAAAGATGCATGGCCAAATGATAACGATAAATGGATTCAATTTGTGGACATAGATGGACTTACTATCAATGGAGGAGGAAAAATTGAACGGCCAAGGTTCTCTTTGGTGGGAAGGTTGTGAAGAACACTGCGATCACCCAACGGTAAAATTTAAAATTTCTTTACGTTTTAATTTACATTGAGTATATAACTAAAATTTGATATTTATAATATATGATTTTTTCAGGCATTATTTTTCCACAATTGCAATGGGCTTCATTTACAGAATACGGAACACATTAACAGTGCCAAGAATCACATCTCCATAAATTTTTGCAATGATGTCACTGTCTCAGATATTCATGTTAATGCTCCCGAAGACAGTCCTAATACAGATGGAATTGACATTTCTAGATCAACCAATGTTATTATTCAAAATTCTTTCATGTTAACCGGTAACAAATTAGTATTATTCTTGGGGACTATTGTTTTTATATATATGATTTTAATGCATATTGTACAAAAATAATTATATTTATACACTTTTATTGAAGGTGATGATTGTATTGCGATTAACAACGGATCTTCCTACATTACTATCAAGGGGGTCACTTGCGGCCCCGGTCATGGTATAAGGTAAAAATATCATTTAAAAAAAAATATATAAAATCTAATCTTATGAACTGTTTTTAATAATTATTTCCCTATCTAATTTTGTATGGTTTTAATTGAATGTAGCGTTGGGAGCTTGGGAGAAGATGGGCAATTCAACTCTGTCGAAAATATCTATGTGAGCGATACCCTTTTGAAGGGAACTCAAAATGGAGTTAGAATCAAGACGTGGGAGGTGATAATTAGTTTTAATTAATTTTATGTATACTATAAAAAATTAATTATTTTTTTTTAACGTAATTACAGAGCGGATATGGATATGCAAGGAACATCACATTTGAACAAATAACATTCGAGGATGTAAAAAACCCGATCATTATTGATCAATATTACACATCTTTTGCATCTACAAGGGAAAATAAGGTACATTAAATTACATTTTTTAAATCTTTTTTTTCATCCTATTTTGACGTGAATATATTTTTTTTTAAATTATGTTTACTTATTTATAGGAAATGGGCATAAAGGTGAGCGATGTGACTTATCGAGGAGTTAATGGAACATCAGCAGATGAAAACGTAATTACTTTCAAGTGTTCTCAAGCTAGTTGCACCAATATTATATTGGACCATGTGGACATACAAATGTCGAATCCCGACGACGAAGCTAAAGTGTTTTGTCAAAATGTTATTGGAAAAGCGAAATCTGTCGTTCCTATTGTTCCTTGCTTATCAGAATTTTAG

mRNA sequence

ATGAAGCTTATTTTTGTGTCGGGTTTTATCTTTTTCACTGTCATTTTAAGTTCGTGGACTGTATTAGCTTCATCTTCTTTCAATGTTCTCGATTATGGAGCTGCTGGAAATGGCGAAACTGATGATACAGAAGCTTTTGTGAAAGCTTGGAAGGATGTCTGTGAAGCAACTGATGACATTCCAACATTGCAAGTACCTGCTACAAAAATTTACCTTTTGAACCCTATCACATTTGAAGGTCCTTGCAAGTCTGAACAAGTCAATTTTGAGCTTAAAGGAACTCTAATGGCTCCGAGCAAAGATGCATGGCCAAATGATAACGATAAATGGATTCAATTTGTGGACATAGATGGACTTACTATCAATGGAGGAGGAAAAATTGAACGGCCAAGTGCCAAGAATCACATCTCCATAAATTTTTGCAATGATGTCACTGTCTCAGATATTCATGTTAATGCTCCCGAAGACAGTCCTAATACAGATGGAATTGACATTTCTAGATCAACCAATGTTATTATTCAAAATTCTTTCATGTTAACCGGTGATGATTGTATTGCGATTAACAACGGATCTTCCTACATTACTATCAAGGGGGTCACTTGCGGCCCCGGTCATGGTATAAGCGTTGGGAGCTTGGGAGAAGATGGGCAATTCAACTCTGTCGAAAATATCTATGTGAGCGATACCCTTTTGAAGGGAACTCAAAATGGAGTTAGAATCAAGACGTGGGAGAGCGGATATGGATATGCAAGGAACATCACATTTGAACAAATAACATTCGAGGATGTAAAAAACCCGATCATTATTGATCAATATTACACATCTTTTGCATCTACAAGGGAAAATAAGGAAATGGGCATAAAGGTGAGCGATGTGACTTATCGAGGAGTTAATGGAACATCAGCAGATGAAAACGTAATTACTTTCAAGTGTTCTCAAGCTAGTTGCACCAATATTATATTGGACCATGTGGACATACAAATGTCGAATCCCGACGACGAAGCTAAAGTGTTTTGTCAAAATGTTATTGGAAAAGCGAAATCTGTCGTTCCTATTGTTCCTTGCTTATCAGAATTTTAG

Coding sequence (CDS)

ATGAAGCTTATTTTTGTGTCGGGTTTTATCTTTTTCACTGTCATTTTAAGTTCGTGGACTGTATTAGCTTCATCTTCTTTCAATGTTCTCGATTATGGAGCTGCTGGAAATGGCGAAACTGATGATACAGAAGCTTTTGTGAAAGCTTGGAAGGATGTCTGTGAAGCAACTGATGACATTCCAACATTGCAAGTACCTGCTACAAAAATTTACCTTTTGAACCCTATCACATTTGAAGGTCCTTGCAAGTCTGAACAAGTCAATTTTGAGCTTAAAGGAACTCTAATGGCTCCGAGCAAAGATGCATGGCCAAATGATAACGATAAATGGATTCAATTTGTGGACATAGATGGACTTACTATCAATGGAGGAGGAAAAATTGAACGGCCAAGTGCCAAGAATCACATCTCCATAAATTTTTGCAATGATGTCACTGTCTCAGATATTCATGTTAATGCTCCCGAAGACAGTCCTAATACAGATGGAATTGACATTTCTAGATCAACCAATGTTATTATTCAAAATTCTTTCATGTTAACCGGTGATGATTGTATTGCGATTAACAACGGATCTTCCTACATTACTATCAAGGGGGTCACTTGCGGCCCCGGTCATGGTATAAGCGTTGGGAGCTTGGGAGAAGATGGGCAATTCAACTCTGTCGAAAATATCTATGTGAGCGATACCCTTTTGAAGGGAACTCAAAATGGAGTTAGAATCAAGACGTGGGAGAGCGGATATGGATATGCAAGGAACATCACATTTGAACAAATAACATTCGAGGATGTAAAAAACCCGATCATTATTGATCAATATTACACATCTTTTGCATCTACAAGGGAAAATAAGGAAATGGGCATAAAGGTGAGCGATGTGACTTATCGAGGAGTTAATGGAACATCAGCAGATGAAAACGTAATTACTTTCAAGTGTTCTCAAGCTAGTTGCACCAATATTATATTGGACCATGTGGACATACAAATGTCGAATCCCGACGACGAAGCTAAAGTGTTTTGTCAAAATGTTATTGGAAAAGCGAAATCTGTCGTTCCTATTGTTCCTTGCTTATCAGAATTTTAG

Protein sequence

MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTINGGGKIERPSAKNHISINFCNDVTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNVIGKAKSVVPIVPCLSEF
Homology
BLAST of Csor.00g245380 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 9.1e-81
Identity = 170/392 (43.37%), Postives = 231/392 (58.93%), Query Frame = 0

Query: 2   KLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIP 61
           K  F++  +FF  I +S     S++ +V  +GA G+G TDD++AF+KAW+ VC  T D  
Sbjct: 4   KTWFLNFSLFFLQIFTS-----SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGD-G 63

Query: 62  TLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTI 121
              VPA   ++L P+ F+G CKS  V  ++ G L+APSK  W  D D+WI F DI+GL I
Sbjct: 64  QFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 123

Query: 122 NGGGKI------------ERPSAKN--------------------HISINFCNDVTVSDI 181
            G G+I             RP+A                      HI I+ CN VT+S +
Sbjct: 124 EGDGEINGQGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSL 183

Query: 182 HVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISV 241
            +NAPE SPNTDGID+  S+NV+IQ+  + TGDDCIAIN+G+S I I G+ CGPGHGIS+
Sbjct: 184 RINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISI 243

Query: 242 GSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIII 301
           GSLG+DG+  +VEN+ V +   +GT NG RIKTW+ G GYAR ITF  IT ++V+NPIII
Sbjct: 244 GSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIII 303

Query: 302 DQYYTSFAS--TRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQ-ASCTNIILDHVDI 357
           DQ+Y    S   ++ K   ++VS V +    GTS  E  + F+CS+   CT I L  + I
Sbjct: 304 DQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKI 363

BLAST of Csor.00g245380 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-64
Identity = 143/363 (39.39%), Postives = 196/363 (53.99%), Query Frame = 0

Query: 25  SSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEGPCKS 84
           +  +V D+GA G+G TDDT+AF KAWKD C +      L VP  K YLL  ITF GPCKS
Sbjct: 83  TDISVDDFGARGDG-TDDTKAFEKAWKDACSSGS---VLIVPENKNYLLKQITFSGPCKS 142

Query: 85  EQVNFELKGTLMAPSKDA-WPNDNDK-WIQFVDIDGLTINGGGKIE-------------- 144
           + +  +++GT+ A S  + W   N K WI+F DI  LT+ GGG                 
Sbjct: 143 D-LRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRK 202

Query: 145 ---------------------------RPSAKNHISINFCNDVTVSDIHVNAPEDSPNTD 204
                                      + S K H+S + C DV  S++ V APE SPNTD
Sbjct: 203 KSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTD 262

Query: 205 GIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSV 264
           GI I+ +  + + NS + TGDDCI+I +GS  +    +TCGPGHGIS+GSLG+      V
Sbjct: 263 GIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHV 322

Query: 265 ENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRE 324
             + V    L  T NG+RIKTW+ G G A+NI F+ I   +V NPIIIDQYY        
Sbjct: 323 SGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCP 382

Query: 325 NKEMGIKVSDVTYRGVNGTSADENVITFKCSQAS-CTNIILDHVDIQMSNPDDEAKVFCQ 344
            +E  +KVS+V Y  + GTSA E  + F CS++S C   I+ ++++ + N   E  + C 
Sbjct: 383 EQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINL-VGNGGKETTMSCS 439

BLAST of Csor.00g245380 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.5e-64
Identity = 142/375 (37.87%), Postives = 194/375 (51.73%), Query Frame = 0

Query: 28  NVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEGPCKSEQV 87
           +V ++GA GNG TDDT+AF  AWK  C +      L VP     LL PI   GPCK+ ++
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRIL-VPENYTCLLRPIDLSGPCKA-RL 113

Query: 88  NFELKGTLMAPS-KDAWPNDN-DKWIQFVDIDGLTINGGGKI--------ERPSAKN--- 147
             ++ GT++AP+  D W   N  KW+ F  +  LT+ GGG +        ER    N   
Sbjct: 114 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 173

Query: 148 ------------------------------HISINFCNDVTVSDIHVNAPEDSPNTDGID 207
                                         HI++  C  VT+S + V AP  SPNTDGI 
Sbjct: 174 PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIH 233

Query: 208 ISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENI 267
           IS S  ++I N+ + TGDDCI+I   S+ I+I  + CGPGHGIS+GSLG+   +  V +I
Sbjct: 234 ISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDI 293

Query: 268 YVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKE 327
            V   ++  T NGVRIKTW+ G G    I F  I   +V NPIIIDQYY        N+ 
Sbjct: 294 TVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQT 353

Query: 328 MGIKVSDVTYRGVNGTSADENVITFKCSQAS-CTNIILDHVDIQMSNPDDEAKVFCQNVI 359
             I + ++++  V GTSA +  I   CS +S C NI+L  +D++ SN D   + FC    
Sbjct: 354 SAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAY 413

BLAST of Csor.00g245380 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.9e-64
Identity = 146/359 (40.67%), Postives = 208/359 (57.94%), Query Frame = 0

Query: 13  TVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYL 72
           ++I S  T L +S+ +V ++GA G+G+TDDT+AF KAWK  C +T+ + T  VP  K YL
Sbjct: 54  SLISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYL 113

Query: 73  LNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTINGGG------- 132
           L    F GPCKS + NF++ GTL A +K +   D + W+   D++ L+I+GG        
Sbjct: 114 LKSTRFRGPCKSLR-NFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGN 173

Query: 133 ---------KIER-------PSA----------------KN----HISINFCNDVTVSDI 192
                    KI++       P+A                KN     ISI  CN V VS++
Sbjct: 174 GKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNV 233

Query: 193 HVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISV 252
            + AP DSPNTDGI I+ + N+ + NS + TGDDCI+I +G+  + I  +TCGPGHGIS+
Sbjct: 234 EITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISI 293

Query: 253 GSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIII 312
           GSLG+D     V  I V       + NGVRIKT++ G G A+NI F+ I  E+VKNPIII
Sbjct: 294 GSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIII 353

Query: 313 DQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQA-SCTNIILDHVDIQ 328
           DQ Y       E++E  ++V +V Y+ ++GTSA +  IT  CS+   C  I+L++V I+
Sbjct: 354 DQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIK 409

BLAST of Csor.00g245380 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 2.5e-62
Identity = 139/347 (40.06%), Postives = 192/347 (55.33%), Query Frame = 0

Query: 25  SSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEGPCKS 84
           ++ +V D+GA G+G+TDDT+AFV AWK  C +   +  L VP    YLL  I   GPC S
Sbjct: 66  TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAV-NLLVPKGNTYLLKSIQLTGPCNS 125

Query: 85  EQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTINGG--GKIE-------------- 144
             +  ++ GTL A  K +   D  KWI F  ++ L+++GG  G ++              
Sbjct: 126 -ILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRN 185

Query: 145 ---------------------------RPSAKNHISINFCNDVTVSDIHVNAPEDSPNTD 204
                                      R + +  ISI  C++V VS++ V AP DSPNTD
Sbjct: 186 KAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTD 245

Query: 205 GIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSV 264
           GI I+ + N+ +  S + TGDDCI+I +GS  + I  +TCGPGHGIS+GSLG+D     V
Sbjct: 246 GIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFV 305

Query: 265 ENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRE 324
             + V    L GT NGVRIKT++ G G A NI F+ I  ++VKNPIIIDQ Y   +    
Sbjct: 306 SGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTT 365

Query: 325 NKEMGIKVSDVTYRGVNGTSADENVITFKCSQ-ASCTNIILDHVDIQ 328
            K   ++V +V YR ++GTSA EN ITF CS+   C  I+LD V+I+
Sbjct: 366 EKS-AVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK 409

BLAST of Csor.00g245380 vs. NCBI nr
Match: KAG6576844.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 727 bits (1877), Expect = 1.06e-264
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI
Sbjct: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120
           PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT
Sbjct: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120

Query: 121 INGGGKIERPSAKNHISINFCNDVTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLT 180
           INGGGKIERPSAKNHISINFCNDVTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLT
Sbjct: 121 INGGGKIERPSAKNHISINFCNDVTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLT 180

Query: 181 GDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRI 240
           GDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRI
Sbjct: 181 GDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRI 240

Query: 241 KTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGT 300
           KTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGT
Sbjct: 241 KTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGT 300

Query: 301 SADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNVIGKAKSVVPIVPCLSEF 359
           SADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNVIGKAKSVVPIVPCLSEF
Sbjct: 301 SADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNVIGKAKSVVPIVPCLSEF 359

BLAST of Csor.00g245380 vs. NCBI nr
Match: KAG7014864.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 697 bits (1798), Expect = 4.35e-252
Identity = 354/394 (89.85%), Postives = 357/394 (90.61%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI
Sbjct: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120
           PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT
Sbjct: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120

Query: 121 INGGGKI---------------ERP--------------------SAKNHISINFCNDVT 180
           INGGGK+               + P                    SAKNHISINFCNDVT
Sbjct: 121 INGGGKMNGQGSLWWEGCEEHCDHPTALFFHNCNGLHLQNTEHIDSAKNHISINFCNDVT 180

Query: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240
           VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH
Sbjct: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240

Query: 241 GISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300
           GISVGSLGEDGQFNSVE+IYVSDTLLKGTQNGVRIK WESGYGYARNITFEQITFEDVKN
Sbjct: 241 GISVGSLGEDGQFNSVESIYVSDTLLKGTQNGVRIKMWESGYGYARNITFEQITFEDVKN 300

Query: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVD 359
           PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVD
Sbjct: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVD 360

BLAST of Csor.00g245380 vs. NCBI nr
Match: XP_022985111.1 (probable polygalacturonase At3g15720 [Cucurbita maxima])

HSP 1 Score: 639 bits (1648), Expect = 1.92e-229
Identity = 319/377 (84.62%), Postives = 332/377 (88.06%), Query Frame = 0

Query: 18  SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPIT 77
           SW VLASSSFN+LDYGAAGNGETDDTEAFVKAWKDVCEATD IPTLQVP  KIYLLNPIT
Sbjct: 5   SWAVLASSSFNILDYGAAGNGETDDTEAFVKAWKDVCEATDGIPTLQVPVAKIYLLNPIT 64

Query: 78  FEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTINGGGKI---------- 137
           F+GPCKSEQVNFELKGTLMAPSKDAWP+DNDKWIQFVDIDGLTINGGGK+          
Sbjct: 65  FQGPCKSEQVNFELKGTLMAPSKDAWPSDNDKWIQFVDIDGLTINGGGKMNGQGSLWWKG 124

Query: 138 -----ERP--------------------SAKNHISINFCNDVTVSDIHVNAPEDSPNTDG 197
                + P                    SAKNHISINFC+DV VSDIHVNAPEDSPNTDG
Sbjct: 125 CEEHCDHPTALFFHNCNGLHLQNTKHIDSAKNHISINFCDDVIVSDIHVNAPEDSPNTDG 184

Query: 198 IDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE 257
           IDISRSTNV+IQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE
Sbjct: 185 IDISRSTNVMIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE 244

Query: 258 NIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTREN 317
           NIYVSD+LLKGTQNGVRIKTWESGYGYARNITFE+ITFEDVKNPIIIDQYYTSFASTREN
Sbjct: 245 NIYVSDSLLKGTQNGVRIKTWESGYGYARNITFEKITFEDVKNPIIIDQYYTSFASTREN 304

Query: 318 KEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNV 359
           KE GIKVSDVTYR VNGTSA ENVITFKCS+ASCTNIILDHVDIQMS+PDDE KVFCQNV
Sbjct: 305 KETGIKVSDVTYREVNGTSAGENVITFKCSEASCTNIILDHVDIQMSHPDDEPKVFCQNV 364

BLAST of Csor.00g245380 vs. NCBI nr
Match: XP_022922555.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 586 bits (1510), Expect = 5.51e-209
Identity = 301/348 (86.49%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKLIF+SGFIFFTV   SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI
Sbjct: 1   MKLIFLSGFIFFTV---SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120
           PTLQVPA KIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT
Sbjct: 61  PTLQVPAAKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120

Query: 121 INGGGKI---------------ERP--------------------SAKNHISINFCNDVT 180
           INGGGKI               + P                    SAKNHISINFCNDVT
Sbjct: 121 INGGGKINGQGSLWWEGCEEHCDHPTALFFHNCNGLHLQNTEHIDSAKNHISINFCNDVT 180

Query: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240
           VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH
Sbjct: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240

Query: 241 GISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300
           GISVGSLGEDGQFNSVENIYVSD+LLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN
Sbjct: 241 GISVGSLGEDGQFNSVENIYVSDSLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300

Query: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQ 313
           PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITF   +
Sbjct: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFNLEE 345

BLAST of Csor.00g245380 vs. NCBI nr
Match: XP_023552837.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573 bits (1477), Expect = 8.13e-204
Identity = 302/393 (76.84%), Postives = 310/393 (78.88%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEA DDI
Sbjct: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEAMDDI 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120
           PTLQVPA KIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWP+DNDKWIQFVDIDGLT
Sbjct: 61  PTLQVPAAKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPSDNDKWIQFVDIDGLT 120

Query: 121 INGGGKI---------------ERP--------------------SAKNHISINFCNDVT 180
           INGGGK+               + P                    SAKNHISINFC+DVT
Sbjct: 121 INGGGKMNGQGSLWWKGCEEHCDHPTALFFHNCNGLHLQNTEHIDSAKNHISINFCDDVT 180

Query: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240
           VSDIH+NAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH
Sbjct: 181 VSDIHINAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240

Query: 241 GISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300
           GISVGSLGEDGQFNSVENIYVSD+LLK TQNGVRIKTWE                     
Sbjct: 241 GISVGSLGEDGQFNSVENIYVSDSLLKRTQNGVRIKTWE--------------------- 300

Query: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVD 358
                             EMGIKV DVTYRGVNGTSADENVITFKCSQA CTNIILDHVD
Sbjct: 301 ------------------EMGIKVRDVTYRGVNGTSADENVITFKCSQARCTNIILDHVD 354

BLAST of Csor.00g245380 vs. ExPASy TrEMBL
Match: A0A6J1J777 (probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111483192 PE=3 SV=1)

HSP 1 Score: 639 bits (1648), Expect = 9.28e-230
Identity = 319/377 (84.62%), Postives = 332/377 (88.06%), Query Frame = 0

Query: 18  SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPIT 77
           SW VLASSSFN+LDYGAAGNGETDDTEAFVKAWKDVCEATD IPTLQVP  KIYLLNPIT
Sbjct: 5   SWAVLASSSFNILDYGAAGNGETDDTEAFVKAWKDVCEATDGIPTLQVPVAKIYLLNPIT 64

Query: 78  FEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTINGGGKI---------- 137
           F+GPCKSEQVNFELKGTLMAPSKDAWP+DNDKWIQFVDIDGLTINGGGK+          
Sbjct: 65  FQGPCKSEQVNFELKGTLMAPSKDAWPSDNDKWIQFVDIDGLTINGGGKMNGQGSLWWKG 124

Query: 138 -----ERP--------------------SAKNHISINFCNDVTVSDIHVNAPEDSPNTDG 197
                + P                    SAKNHISINFC+DV VSDIHVNAPEDSPNTDG
Sbjct: 125 CEEHCDHPTALFFHNCNGLHLQNTKHIDSAKNHISINFCDDVIVSDIHVNAPEDSPNTDG 184

Query: 198 IDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE 257
           IDISRSTNV+IQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE
Sbjct: 185 IDISRSTNVMIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE 244

Query: 258 NIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTREN 317
           NIYVSD+LLKGTQNGVRIKTWESGYGYARNITFE+ITFEDVKNPIIIDQYYTSFASTREN
Sbjct: 245 NIYVSDSLLKGTQNGVRIKTWESGYGYARNITFEKITFEDVKNPIIIDQYYTSFASTREN 304

Query: 318 KEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVDIQMSNPDDEAKVFCQNV 359
           KE GIKVSDVTYR VNGTSA ENVITFKCS+ASCTNIILDHVDIQMS+PDDE KVFCQNV
Sbjct: 305 KETGIKVSDVTYREVNGTSAGENVITFKCSEASCTNIILDHVDIQMSHPDDEPKVFCQNV 364

BLAST of Csor.00g245380 vs. ExPASy TrEMBL
Match: A0A6J1E943 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430523 PE=3 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 2.67e-209
Identity = 301/348 (86.49%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKLIF+SGFIFFTV   SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI
Sbjct: 1   MKLIFLSGFIFFTV---SWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120
           PTLQVPA KIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT
Sbjct: 61  PTLQVPAAKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLT 120

Query: 121 INGGGKI---------------ERP--------------------SAKNHISINFCNDVT 180
           INGGGKI               + P                    SAKNHISINFCNDVT
Sbjct: 121 INGGGKINGQGSLWWEGCEEHCDHPTALFFHNCNGLHLQNTEHIDSAKNHISINFCNDVT 180

Query: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240
           VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH
Sbjct: 181 VSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGH 240

Query: 241 GISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300
           GISVGSLGEDGQFNSVENIYVSD+LLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN
Sbjct: 241 GISVGSLGEDGQFNSVENIYVSDSLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKN 300

Query: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQ 313
           PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITF   +
Sbjct: 301 PIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFNLEE 345

BLAST of Csor.00g245380 vs. ExPASy TrEMBL
Match: A0A1S3BYT5 (probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=3 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 1.46e-169
Identity = 241/384 (62.76%), Postives = 293/384 (76.30%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKL+  +   +   ILSS TVL+SS FNVLDYGA GNG+TDD++AF+KAW DVC AT D 
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDN-DKWIQFVDIDGL 120
           PTL VP  K YLL+P+ F+GPCKS QV F+L GTLMAP+KD WPND   KW+QFVD++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TINGGGKIERP-------------------------SAKNHISINFCNDVTVSDIHVNAP 180
            + GGG+ +                           SAKNHISIN CNDV  S +H++AP
Sbjct: 121 NVQGGGRFDGQGSCWWKALFFHNCNGLQMNGMKHVNSAKNHISINLCNDVIFSHLHISAP 180

Query: 181 EDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGE 240
           EDSPNTDGIDIS+STN+ IQ+SF+ TGDDC+AINNGSS I I  +TCGPGHGIS+GSLG+
Sbjct: 181 EDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPGHGISIGSLGK 240

Query: 241 DGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYT 300
           DG++N VEN++VS+ LL+ TQNG+RIKTWE GYGYA+NITFE+IT ++ KNPIIIDQYYT
Sbjct: 241 DGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSKNPIIIDQYYT 300

Query: 301 SFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDHVDIQMSNPDDE 358
           S+A +R+ K M IKVSDVTYR VNGTSA+E  ITF CSQA C+NIILD+V+I+M+NP +E
Sbjct: 301 SYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNVNIKMTNPGEE 360

BLAST of Csor.00g245380 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 2.49e-168
Identity = 243/395 (61.52%), Postives = 295/395 (74.68%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKL+  +   +   ILSS TVL+SS FNVLDYGA GNG+TDD++AF+KAW DVC AT D 
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDN-DKWIQFVDIDGL 120
           PTL VP  K YLL+P+ F+GPCKS QV F+L GTLMAP+KD WPND   KW+QFVD++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TINGGGKIE----------------RP--------------------SAKNHISINFCND 180
            + GGG+ +                RP                    SAKNHISIN CND
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGP 240
           V  S +H++APEDSPNTDGIDIS+STN+ IQ+SF+ TGDDC+AINNGSS I I  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDV 300
           GHGIS+GSLG+DG++N VEN++VS+ LL+ TQNG+RIKTWE GYGYA+NITFE+IT ++ 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDH 358
           KNPIIIDQYYTS+A +R+ K M IKVSDVTYR VNGTSA+E  ITF CSQA C+NIILD+
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

BLAST of Csor.00g245380 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 1.36e-164
Identity = 239/395 (60.51%), Postives = 294/395 (74.43%), Query Frame = 0

Query: 1   MKLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDI 60
           MKL+  + F++   ILSSWTVL SS FNVLDYGA GNG+TDD++AF+KAW DVC AT++ 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDND-KWIQFVDIDGL 120
           PTL VP  K YLLNP+ F+GPCKS+Q    L GTLMAP+KD W +D   +WIQF DI+GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 TINGGGKIE----------------RP--------------------SAKNHISINFCND 180
            + GGG+ +                RP                    SAKNHISIN C+D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 VTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGP 240
           V  S +H++APEDSPNTDGIDIS STN+ I++SFM TGDDCIAINNGSS I I G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDV 300
           GHGIS+GSLG+DG++N VEN++VS+ LL+ TQNG+RIKTWE GYGYA+NITFE+IT ++ 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQASCTNIILDH 358
           KNPIIIDQYY+S+A +R+ K M IKVSDVTYR VNGTSA+E+ ITF CSQA C+NIILD+
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

BLAST of Csor.00g245380 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.8e-82
Identity = 169/389 (43.44%), Postives = 232/389 (59.64%), Query Frame = 0

Query: 2   KLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIP 61
           K  F++  +FF  I +S     S++ +V  +GA G+G TDD++AF+KAW+ VC  T D  
Sbjct: 3   KTWFLNFSLFFLQIFTS-----SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGD-G 62

Query: 62  TLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTI 121
              VPA   ++L P+ F+G CKS  V  ++ G L+APSK  W  D D+WI F DI+GL I
Sbjct: 63  QFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 122

Query: 122 NGGGKI------------ERPSAKN-----------------HISINFCNDVTVSDIHVN 181
            G G+I             RP+ ++                 HI I+ CN VT+S + +N
Sbjct: 123 EGDGEINGQGSSWWEHKGSRPTFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRIN 182

Query: 182 APEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSL 241
           APE SPNTDGID+  S+NV+IQ+  + TGDDCIAIN+G+S I I G+ CGPGHGIS+GSL
Sbjct: 183 APESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSL 242

Query: 242 GEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQY 301
           G+DG+  +VEN+ V +   +GT NG RIKTW+ G GYAR ITF  IT ++V+NPIIIDQ+
Sbjct: 243 GKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQF 302

Query: 302 YTSFAS--TRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQ-ASCTNIILDHVDIQ-- 357
           Y    S   ++ K   ++VS V +    GTS  E  + F+CS+   CT I L  + I+  
Sbjct: 303 YNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETA 362

BLAST of Csor.00g245380 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.8e-82
Identity = 160/348 (45.98%), Postives = 216/348 (62.07%), Query Frame = 0

Query: 27  FNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEGPCKSEQ 86
           +NVL +GA G+G+TDD+ AF++AW   C   ++I TL +P+ K YLL PI F+GPCKS  
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  VNFELKGTLMAPSK-DAWPNDNDK-WIQFVDIDGLTINGGGKIER--------------- 146
           +  +L G ++APS   +W N   + WI F  + GL I+G G I                 
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWEALHICNCN 172

Query: 147 ----------PSAKNHISINFCNDVTVSDIHVNAPEDSPNTDGIDISRSTNVIIQNSFML 206
                      S K+HISI  C+ V +S I++ APE+SPNTDGIDIS STNV I +S + 
Sbjct: 173 NLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYSTNVNIFDSTIQ 232

Query: 207 TGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVENIYVSDTLLKGTQNGVR 266
           TGDDCIAIN GSS I I  V CGPGHGISVGSLG DG+  +V ++YV+      T NG R
Sbjct: 233 TGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQCTFNKTTNGAR 292

Query: 267 IKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENKEMGIKVSDVTYRGVNG 326
           IKTW+ G GYARNI+FE IT  +V+NPIIIDQ YT        K+  + +S V Y G  G
Sbjct: 293 IKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAISSVKYVGFQG 352

Query: 327 TSADENVITFKCSQAS-CTNIILDHVDIQMSNPDDEAKVFCQNVIGKA 347
           T+ +E+ I  KCS  + C ++++D +++ M N  ++ KV C+NV G++
Sbjct: 353 TTLNEDAIMLKCSAITYCKDMVIDDIEVTMEN-GEKPKVECENVEGES 399

BLAST of Csor.00g245380 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.5e-82
Identity = 170/392 (43.37%), Postives = 231/392 (58.93%), Query Frame = 0

Query: 2   KLIFVSGFIFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIP 61
           K  F++  +FF  I +S     S++ +V  +GA G+G TDD++AF+KAW+ VC  T D  
Sbjct: 4   KTWFLNFSLFFLQIFTS-----SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGD-G 63

Query: 62  TLQVPATKIYLLNPITFEGPCKSEQVNFELKGTLMAPSKDAWPNDNDKWIQFVDIDGLTI 121
              VPA   ++L P+ F+G CKS  V  ++ G L+APSK  W  D D+WI F DI+GL I
Sbjct: 64  QFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 123

Query: 122 NGGGKI------------ERPSAKN--------------------HISINFCNDVTVSDI 181
            G G+I             RP+A                      HI I+ CN VT+S +
Sbjct: 124 EGDGEINGQGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSL 183

Query: 182 HVNAPEDSPNTDGIDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISV 241
            +NAPE SPNTDGID+  S+NV+IQ+  + TGDDCIAIN+G+S I I G+ CGPGHGIS+
Sbjct: 184 RINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISI 243

Query: 242 GSLGEDGQFNSVENIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIII 301
           GSLG+DG+  +VEN+ V +   +GT NG RIKTW+ G GYAR ITF  IT ++V+NPIII
Sbjct: 244 GSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIII 303

Query: 302 DQYYTSFAS--TRENKEMGIKVSDVTYRGVNGTSADENVITFKCSQ-ASCTNIILDHVDI 357
           DQ+Y    S   ++ K   ++VS V +    GTS  E  + F+CS+   CT I L  + I
Sbjct: 304 DQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKI 363

BLAST of Csor.00g245380 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-80
Identity = 163/364 (44.78%), Postives = 222/364 (60.99%), Query Frame = 0

Query: 21  VLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATKIYLLNPITFEG 80
           ++   ++NVL++ A G+G+TDD+EAF++AW   C    DI TL +P+ K +LL P  F+G
Sbjct: 18  IVYGKNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQG 77

Query: 81  PCKSEQVNFELKGTLMAPS-KDAWPNDNDK-WIQFVDIDGLTINGGGKI----------- 140
           PCKS  +  +L GT++APS K AW +   + WI+F  + GL I G G I           
Sbjct: 78  PCKSSSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELN 137

Query: 141 ----ERPSA--------------------KNHISINFCNDVTVSDIHVNAPEDSPNTDGI 200
               +RP+A                    KNHISI  CN V +S+I++ APE SPNTDGI
Sbjct: 138 LKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGI 197

Query: 201 DISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVEN 260
           DIS STN+ I +S + TGDDCIAIN+GSS I I G+ CGPGHGISVGSLG  G    V +
Sbjct: 198 DISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSD 257

Query: 261 IYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTRENK 320
           + V+      T NG RIKTW  G GYARNI+F  IT  + KNPIIIDQ+Y      R  +
Sbjct: 258 VQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHYID--KGRLTE 317

Query: 321 EMGIKVSDVTYRGVNGTSADENVITFKCSQAS-CTNIILDHVDIQMSNPDDEAKVFCQNV 347
           E  + +S+V +    GTS+++N IT KCS+ + C ++++D +DI M+N   + KV CQ V
Sbjct: 318 ESAVAISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMAN-GGKPKVNCQYV 377

BLAST of Csor.00g245380 vs. TAIR 10
Match: AT5G44840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.3e-74
Identity = 153/365 (41.92%), Postives = 222/365 (60.82%), Query Frame = 0

Query: 10  IFFTVILSSWTVLASSSFNVLDYGAAGNGETDDTEAFVKAWKDVCEATDDIPTLQVPATK 69
           +F   +L S  ++    ++VL++GA GNG TDD++AFVKAW  +C +  +  T  +P+ K
Sbjct: 8   VFLLTLLHS-ILVNGQIYDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNK 67

Query: 70  IYLLNPITFEGPCKSEQVNFELKGTLMAP-SKDAWPNDN-DKWIQFVDIDGLTINGGGKI 129
            +LL P+TF+GPCKS  V  +  G ++AP +K AW +    +W+ F +I GLT+NG G I
Sbjct: 68  TFLLQPLTFQGPCKSPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTI 127

Query: 130 ER-------------------------PSAKNHISINFCNDVTVSDIHVNAPEDSPNTDG 189
                                       S KNHISI+ C  V ++ I + AP DSPNTDG
Sbjct: 128 HGRGSSFWKQLHFQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLVAPHDSPNTDG 187

Query: 190 IDISRSTNVIIQNSFMLTGDDCIAINNGSSYITIKGVTCGPGHGISVGSLGEDGQFNSVE 249
           I+IS S++V I ++ + TGDDC+AIN+GS  I I  + CGPGHGISVGS+G DG+ + VE
Sbjct: 188 INISESSDVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSVGRDGEESIVE 247

Query: 250 NIYVSDTLLKGTQNGVRIKTWESGYGYARNITFEQITFEDVKNPIIIDQYYTSFASTREN 309
           N+ V++     T NG RIKTW +G GYA+NI F+ +TF + KNPIIIDQ Y       + 
Sbjct: 248 NVQVTNCTFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQNYVD-KGRLDV 307

Query: 310 KEMGIKVSDVTYRGVNGTSADENVITFKCSQAS-CTNIILDHVDIQMSNPDDEAKVFCQN 347
           +E  + +S+VT+  + GTS  + +I   CS+ + C +I+LD +DI   +  ++  V C N
Sbjct: 308 EESAVAISNVTFTDLRGTSKLDEIIKIDCSKVTYCKDIVLDKIDIATVD-GNKPIVECSN 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LW079.1e-8143.37Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q020961.2e-6439.39Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q94AJ54.5e-6437.87Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
O231475.9e-6440.67Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Q8RY292.5e-6240.06Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KAG6576844.11.06e-264100.00putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7014864.14.35e-25289.85putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022985111.11.92e-22984.62probable polygalacturonase At3g15720 [Cucurbita maxima][more]
XP_022922555.15.51e-20986.49probable polygalacturonase At3g15720 [Cucurbita moschata][more]
XP_023552837.18.13e-20476.84probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1J7779.28e-23084.62probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111483192... [more]
A0A6J1E9432.67e-20986.49probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305... [more]
A0A1S3BYT51.46e-16962.76probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=... [more]
A0A5D3CYS82.49e-16861.52Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A0A0KZ951.36e-16460.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15720.23.8e-8243.44Pectin lyase-like superfamily protein [more]
AT5G27530.13.8e-8245.98Pectin lyase-like superfamily protein [more]
AT3G15720.16.5e-8243.37Pectin lyase-like superfamily protein [more]
AT4G35670.12.1e-8044.78Pectin lyase-like superfamily protein [more]
AT5G44840.11.3e-7441.92Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 191..211
e-value: 2500.0
score: 4.4
coord: 221..242
e-value: 290.0
score: 10.2
coord: 141..167
e-value: 74.0
score: 12.2
coord: 250..271
e-value: 1700.0
score: 5.6
coord: 168..189
e-value: 1200.0
score: 6.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 61..128
e-value: 4.7E-6
score: 25.9
coord: 133..342
e-value: 5.3E-65
score: 219.6
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 198..211
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 16..130
e-value: 1.8E-22
score: 81.9
coord: 131..353
e-value: 3.8E-70
score: 238.9
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 16..128
coord: 131..356
NoneNo IPR availablePANTHERPTHR31375:SF232SUBFAMILY NOT NAMEDcoord: 16..128
coord: 131..356
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 11..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g245380.m01Csor.00g245380.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity