Csor.00g237230 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g237230
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic-like
LocationCsor_Chr08: 7520743 .. 7521717 (+)
RNA-Seq ExpressionCsor.00g237230
SyntenyCsor.00g237230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCTCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTTCGATATCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTCCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTTGAAGCGGCCATTTTGAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAATGGCAGAACATCCGCCGCCTTCGGAGCAAACGGGGATGGAATCTCTACCGCATTGACGCCACCGAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

mRNA sequence

ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCTCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTTCGATATCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTCCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTTGAAGCGGCCATTTTGAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAATGGCAGAACATCCGCCGCCTTCGGAGCAAACGGGGATGGAATCTCTACCGCATTGACGCCACCGAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

Coding sequence (CDS)

ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCTCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTTCGATATCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTCCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTTGAAGCGGCCATTTTGAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAATGGCAGAACATCCGCCGCCTTCGGAGCAAACGGGGATGGAATCTCTACCGCATTGACGCCACCGAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

Protein sequence

MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILHQFLCQNTYTVNIPTTTIKSNLTVF
Homology
BLAST of Csor.00g237230 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.9e-91
Identity = 174/292 (59.59%), Postives = 224/292 (76.71%), Query Frame = 0

Query: 15  EPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIY 74
           +P  ++FQG N +++ PFFRRK+K+VFVMGATGTGKSRLA+DLATRF AEIVNSDKIQ+Y
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEVVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHATSTINSIVGKHRLPIVAG 134
           KGL++VTNKVT +E  GVPHHLLG + D   +FTA DF+R A   + SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDP 194
           GSNS+IE+LV+   +FR +Y CCFLWVDVS P+LHSFVSERVD+M+D G ++EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 NENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARS--RILEAAILKIKENTRKLAFRQW 254
           + +DY+ GIRRAIGVPELD FLR+EM    +Y A +  R+LE AI KIKENT  LA RQ 
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEM---RNYPAETTERLLETAIEKIKENTCLLACRQL 252

Query: 255 QNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILHQFL 304
           Q I+RL  +  WN++R+DATEVF  R   + +D  W+  V  P++  + +FL
Sbjct: 253 QKIQRLYKQWKWNMHRVDATEVFLRRG--EEADEAWDNSVAHPSALAVEKFL 297

BLAST of Csor.00g237230 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.6e-82
Identity = 152/308 (49.35%), Postives = 217/308 (70.45%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQ----GTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVD 60
           MKIS+ A CK+     P +DF     G N + L P +  K+K+V +MGATGTGKSRL+VD
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAT 120
           +ATRF AEI+NSDKIQ+++GL++VTNK+T +E CGVPHHLLG+  P ++ TAA++   A 
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 STINSIVGKHRLPIVAGGSNSFIESLVDG-DGEFRYQYECCFLWVDVSLPILHSFVSERV 180
            +I S++ + +LPI+ GGSNS++E+LVD  + +FR +Y+CCFLWVDV+LP+LH FVSERV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAA 240
           D+M+++G + EVR  FD + +DY+RGI++AIG PE D F R E     +   R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNE--QFLNVEDREELLSKV 242

Query: 241 ILKIKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPA 300
           + +IK NT +LA RQ + I RLR  + W++ R+DAT VF  R +K  ++  WE LV  P+
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SKILHQFL 304
           +  + +FL
Sbjct: 303 TDTVSRFL 306

BLAST of Csor.00g237230 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 1.5e-79
Identity = 161/313 (51.44%), Postives = 215/313 (68.69%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK S+S S K+   +P L      + +N+  F   K K++FVMGATG+GKSRLA+DLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHATSTI 120
           F  EI+NSDKIQ+YKGL+V+TNKVT KEC GVPHHLLG+ D  + N TA  + R A+  I
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFIESLVD-GDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           + +   ++LPIVAGGSNS+IE+LV+   G     Y+CCF+WVDVSLP+L+SFVS+RVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDNGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILK 240
           ++ G + EVR +F+P  N Y+ GIRRAIGVPEL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 IKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKI 300
           IK+NT  LA RQ + I+RL  K   +++R+DATEVF  R N +  D  WE LV  P+ +I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 LHQFLCQNTYTVN 312
           + +F   N    N
Sbjct: 301 VDKFYNNNNQLKN 306

BLAST of Csor.00g237230 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 132/312 (42.31%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 24  TNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIYKGLEVVTNK 83
           T  ++L+    RK KL+ +MGATGTGKSRL++DLA  F  E++NSDK+Q+YKGL++ TNK
Sbjct: 17  TTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNK 76

Query: 84  VTEKECCGVPHHLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESL 143
           ++  +  GVPHHLLG  DP     T ADFR  A   ++ I G+ +LP++ GGSNSFI +L
Sbjct: 77  ISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHAL 136

Query: 144 V------DGDGEF----------RYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINE 203
           +       G G F            +Y+CCFLWVDVS+ +L  ++++RVD ML+ G  +E
Sbjct: 137 LVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDE 196

Query: 204 VRNLFDPNENDY-----TR-GIRRAIGVPELDAFLR------AEMDSATDYRARSRILEA 263
           +   + P + D+     TR G+R+AIGVPE D +         E +     R R    E 
Sbjct: 197 LAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEE 256

Query: 264 AILKIKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQ---HRDNKQHSDHVWEALV 304
           A+  IKENT  LA RQ   I RL+   GW+L R+DATE F+     D+ +    +WE  V
Sbjct: 257 AVRAIKENTCHLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQV 316

BLAST of Csor.00g237230 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 124/296 (41.89%), Postives = 181/296 (61.15%), Query Frame = 0

Query: 35  RKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPH 94
           RK+K+V ++GATG GKSRL+VDLATRF +EI+NSDKIQ+Y+GLE+ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRY-- 154
           HLLG+ +P +   TA +FR  A++ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDY- 214
                       +YECCF+WVDVS  +L+ ++  RVD M+D+G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 TR-GIRRAIGVPELDAFLR---AEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNI 274
           TR GIR+AIGVPE D + +    E         R    + A+  IK NT  LA RQ + I
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 RRLRSKRGWNLYRIDATEVFQHRDNKQHSD----HVWEALVTEPASKILHQFLCQN 307
             L+   GW + R+DAT  F+    K  S+      WE  V EP+ KI+ + L QN
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of Csor.00g237230 vs. NCBI nr
Match: KAG6593917.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 651 bits (1679), Expect = 1.06e-235
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

BLAST of Csor.00g237230 vs. NCBI nr
Match: KAG7026259.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640 bits (1650), Expect = 2.78e-231
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNL+RIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

BLAST of Csor.00g237230 vs. NCBI nr
Match: XP_023522124.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639 bits (1648), Expect = 5.62e-231
Identity = 318/324 (98.15%), Postives = 321/324 (99.07%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRK+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLP+LHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPVLHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDRNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNIRRLRSKRGWNL+RIDATEVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

BLAST of Csor.00g237230 vs. NCBI nr
Match: XP_022930300.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 637 bits (1642), Expect = 4.61e-230
Identity = 316/324 (97.53%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNT+NLKPFFR+K+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTVNLKPFFRQKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTFADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNIRRLRSKRGWNL+RIDATEVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTT IKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTAIKSNLTVF 324

BLAST of Csor.00g237230 vs. NCBI nr
Match: XP_023513929.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633 bits (1632), Expect = 1.54e-228
Identity = 316/324 (97.53%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTN+INLKPFFRRK+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNSINLKPFFRRKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKEC GVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECRGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLP+LHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPVLHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDRNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNIRRLRSKRGWNL+RIDATEVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

BLAST of Csor.00g237230 vs. ExPASy TrEMBL
Match: A0A6J1EWK5 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436795 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 2.23e-230
Identity = 316/324 (97.53%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNT+NLKPFFR+K+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTVNLKPFFRQKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTFADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNIRRLRSKRGWNL+RIDATEVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTT IKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTAIKSNLTVF 324

BLAST of Csor.00g237230 vs. ExPASy TrEMBL
Match: A0A6J1KF69 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111494482 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 2.77e-217
Identity = 302/324 (93.21%), Postives = 310/324 (95.68%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQ  NTINLKPFFR+K+KLVFVMGATGTGKSRLAV+LATR
Sbjct: 38  MKISLSASCKKAAAEPPLIDFQRRNTINLKPFFRQKDKLVFVMGATGTGKSRLAVNLATR 97

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTA DFR  A STIN
Sbjct: 98  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAVDFRHVAMSTIN 157

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SI+G+HRLPIVAGGSNSFIESLVDGD EFRYQYECCFLW+DVSLPILHSFVSERVDRMLD
Sbjct: 158 SIIGRHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWIDVSLPILHSFVSERVDRMLD 217

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILK K
Sbjct: 218 NGFINEVRNLFDSNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKTK 277

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLR KRGWNL+RIDATEVFQHRDN+Q  DHVWEALVTEPASKILH
Sbjct: 278 ENTRKLAFRQWQNICRLRRKRGWNLHRIDATEVFQHRDNEQQFDHVWEALVTEPASKILH 337

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 338 QFLCQNTYTVNIPTTTIKSNLTVF 361

BLAST of Csor.00g237230 vs. ExPASy TrEMBL
Match: A0A6J1H3C2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460117 PE=4 SV=1)

HSP 1 Score: 510 bits (1313), Expect = 2.99e-180
Identity = 248/306 (81.05%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK SLSASCK+ AAEPPL++ QGTN +NLKPFFR+K+KLVFVMGATGTGKSRLA++LA R
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEI+NSDKIQIYKGLE++TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHA+  IN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVD + EFRY+Y+CCFLWVDVSLP+LHSFVS+RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NG +NEVRNLFDP ENDYTRGIRRAIGVPELDAFL AEMDSATD +ARSRIL AAILKIK
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           EN+ KLA RQ Q I RL+SK GWNL+RIDATEVF   D  ++SDH WEALV EP+S I+ 
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHDGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLCQN 306
           QFLC+N
Sbjct: 301 QFLCEN 306

BLAST of Csor.00g237230 vs. ExPASy TrEMBL
Match: A0A6J1KZJ7 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499016 PE=4 SV=1)

HSP 1 Score: 501 bits (1290), Expect = 9.49e-177
Identity = 243/306 (79.41%), Postives = 273/306 (89.22%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK SLSASCK+AA E PL++FQGTN +NLKPFFRRK+KLVFVMGATGTGKSRLA++LA R
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGTNILNLKPFFRRKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEI+NSDKIQIYKGLE++TNKVTEKECCGVPHHLLGI DPNSNFTA DF RHA+  IN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPI+AGGSNSFIESLVDG+ EFRY+Y+CCFLWVDVSLP+LHSFVS+RVDRMLD
Sbjct: 121 SIVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NG +NEVRNLFDP  NDYTRGIRRAIGVPELDAFLRAEMDSATD +ARS+IL+AAILKIK
Sbjct: 181 NGLVNEVRNLFDPKGNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAILKIK 240

Query: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300
           EN+ KLA RQ Q I  L+SK GWNL+RIDATEVF      ++S+H WEALV EP+S I+ 
Sbjct: 241 ENSIKLALRQRQKIHHLQSKWGWNLHRIDATEVFLQHGGGEYSEHAWEALVAEPSSIIVD 300

Query: 301 QFLCQN 306
           QFLC+N
Sbjct: 301 QFLCEN 306

BLAST of Csor.00g237230 vs. ExPASy TrEMBL
Match: A0A0A0KHK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 3.59e-163
Identity = 242/315 (76.83%), Postives = 278/315 (88.25%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTN-TINLKPFFRRKNKLVFVMGATGTGKSRLAVDLAT 60
           MKISLS SCK+ A EPPL++FQGTN T+NL+PFFRRK+K+VFVMGATGTGKSRLA+DLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTI 120
           RF AEI+NSDKIQ+YKGL+V+TNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHA+STI
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSIV-GKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           NSIV GK  LPI+AGGSNSFIESLV+ + EFRY+Y+CCFLWVDVSLPIL+SFVSERVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LDNGFINEVRNLFDPNE--NDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAI 240
           L+NG +NEVR+LFDPN   NDY  GIRRAIGVPELDAFLRAEMDSATD + RSRIL+AAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTRKLAFRQWQNIRRLRSK-RGWN-LYRIDATEVFQHRDNKQHS-DHVWEALVTE 300
           LKIKENT KLA RQ Q I RL+SK RGWN L+RIDATEVF   D++ +S D+ WE LV +
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PASKILHQFLCQNTY 308
           P+ KI+ QFL +N++
Sbjct: 301 PSFKIVDQFLSENSF 315

BLAST of Csor.00g237230 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 336.3 bits (861), Expect = 2.8e-92
Identity = 174/292 (59.59%), Postives = 224/292 (76.71%), Query Frame = 0

Query: 15  EPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIY 74
           +P  ++FQG N +++ PFFRRK+K+VFVMGATGTGKSRLA+DLATRF AEIVNSDKIQ+Y
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEVVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHATSTINSIVGKHRLPIVAG 134
           KGL++VTNKVT +E  GVPHHLLG + D   +FTA DF+R A   + SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDP 194
           GSNS+IE+LV+   +FR +Y CCFLWVDVS P+LHSFVSERVD+M+D G ++EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 NENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARS--RILEAAILKIKENTRKLAFRQW 254
           + +DY+ GIRRAIGVPELD FLR+EM    +Y A +  R+LE AI KIKENT  LA RQ 
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEM---RNYPAETTERLLETAIEKIKENTCLLACRQL 252

Query: 255 QNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILHQFL 304
           Q I+RL  +  WN++R+DATEVF  R   + +D  W+  V  P++  + +FL
Sbjct: 253 QKIQRLYKQWKWNMHRVDATEVFLRRG--EEADEAWDNSVAHPSALAVEKFL 297

BLAST of Csor.00g237230 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 307.0 bits (785), Expect = 1.8e-83
Identity = 152/308 (49.35%), Postives = 217/308 (70.45%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQ----GTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVD 60
           MKIS+ A CK+     P +DF     G N + L P +  K+K+V +MGATGTGKSRL+VD
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAT 120
           +ATRF AEI+NSDKIQ+++GL++VTNK+T +E CGVPHHLLG+  P ++ TAA++   A 
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 STINSIVGKHRLPIVAGGSNSFIESLVDG-DGEFRYQYECCFLWVDVSLPILHSFVSERV 180
            +I S++ + +LPI+ GGSNS++E+LVD  + +FR +Y+CCFLWVDV+LP+LH FVSERV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAA 240
           D+M+++G + EVR  FD + +DY+RGI++AIG PE D F R E     +   R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNE--QFLNVEDREELLSKV 242

Query: 241 ILKIKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPA 300
           + +IK NT +LA RQ + I RLR  + W++ R+DAT VF  R +K  ++  WE LV  P+
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SKILHQFL 304
           +  + +FL
Sbjct: 303 TDTVSRFL 306

BLAST of Csor.00g237230 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 161/313 (51.44%), Postives = 215/313 (68.69%), Query Frame = 0

Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK S+S S K+   +P L      + +N+  F   K K++FVMGATG+GKSRLA+DLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHATSTI 120
           F  EI+NSDKIQ+YKGL+V+TNKVT KEC GVPHHLLG+ D  + N TA  + R A+  I
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFIESLVD-GDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           + +   ++LPIVAGGSNS+IE+LV+   G     Y+CCF+WVDVSLP+L+SFVS+RVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDNGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILK 240
           ++ G + EVR +F+P  N Y+ GIRRAIGVPEL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 IKENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKI 300
           IK+NT  LA RQ + I+RL  K   +++R+DATEVF  R N +  D  WE LV  P+ +I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 LHQFLCQNTYTVN 312
           + +F   N    N
Sbjct: 301 VDKFYNNNNQLKN 306

BLAST of Csor.00g237230 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 220.7 bits (561), Expect = 1.7e-57
Identity = 124/296 (41.89%), Postives = 181/296 (61.15%), Query Frame = 0

Query: 35  RKNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPH 94
           RK+K+V ++GATG GKSRL+VDLATRF +EI+NSDKIQ+Y+GLE+ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRY-- 154
           HLLG+ +P +   TA +FR  A++ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDY- 214
                       +YECCF+WVDVS  +L+ ++  RVD M+D+G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 TR-GIRRAIGVPELDAFLR---AEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNI 274
           TR GIR+AIGVPE D + +    E         R    + A+  IK NT  LA RQ + I
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 RRLRSKRGWNLYRIDATEVFQHRDNKQHSD----HVWEALVTEPASKILHQFLCQN 307
             L+   GW + R+DAT  F+    K  S+      WE  V EP+ KI+ + L QN
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of Csor.00g237230 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 125/294 (42.52%), Postives = 182/294 (61.90%), Query Frame = 0

Query: 36  KNKLVFVMGATGTGKSRLAVDLATRFSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHH 95
           K K+V +MGATG+GKS L++DLATRFS EIVNSDKIQ Y GL+V TN+++  E CGVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 96  LLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIES-LVDGDGEFRY-- 155
           LLG   P +S  T ++FR  A+ +I+ I  +  LPI+AGGSNSFI + LVD      Y  
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 156 ----------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNEND---Y 215
                     +YECCFLWVDVS+ +L  ++S+RVD+M+++G   E+   +DP  +     
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 216 TRGIRRAIGVPELDAFL------RAEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQ 275
             GI + IG+PE D +       R +  S  D +AR    + A+ +IKENT +LA +Q +
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWD-QARKGAYDEAVQEIKENTWRLAKKQIE 281

Query: 276 NIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILHQFLCQN 307
            I +L+S  GW++ R+DAT  F      + S  +W+  V + + K++ +FL ++
Sbjct: 282 RIMKLKSS-GWDIQRLDATPSF-----GRSSREIWDNTVLDESIKVVKRFLVKD 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94ID23.9e-9159.59Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q93WC92.6e-8249.35Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID11.5e-7951.44Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.4e-5942.31Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID32.4e-5641.89Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
KAG6593917.11.06e-235100.00Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
KAG7026259.12.78e-23198.46Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_023522124.15.62e-23198.15adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
XP_022930300.14.61e-23097.53adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata][more]
XP_023513929.11.54e-22897.53adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
A0A6J1EWK52.23e-23097.53adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KF692.77e-21793.21adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1H3C22.99e-18081.05adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KZJ79.49e-17779.41adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A0A0KHK33.59e-16376.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G19040.12.8e-9259.59isopentenyltransferase 5 [more]
AT3G63110.11.8e-8349.35isopentenyltransferase 3 [more]
AT3G23630.11.1e-8051.44isopentenyltransferase 7 [more]
AT1G68460.11.7e-5741.89isopentenyltransferase 1 [more]
AT3G19160.11.1e-5642.52ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 36..303
e-value: 1.8E-104
score: 350.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 39..148
NoneNo IPR availablePFAMPF01715IPPTcoord: 151..256
e-value: 1.7E-12
score: 47.6
coord: 72..145
e-value: 5.3E-21
score: 75.5
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 164..265
e-value: 1.8E-104
score: 350.7
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 27..308
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 27..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g237230.m01Csor.00g237230.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity