Csor.00g219730 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g219730
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionGEM-like protein 4
LocationCsor_Chr04: 6544216 .. 6545202 (-)
RNA-Seq ExpressionCsor.00g219730
SyntenyCsor.00g219730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATTACAATTCAAGAACATGTTTTTGGAGCTCAGATGAAGTCTTTGGGTCTTGCTCGAGCAAGATTTTTGGCTGATCCCTTCAAGCATCTCCATATGTCTTCTTTAGATGATGGGTCTCAATCAATTAAGAAATGTAAGCTTAAGAAGAACAAAAAAAAGTATTCTTTTTTTGGTTTAATGCATAAATAGTTTGCTGATTTGGGTTTTGAATTGTTGCAGATAATGGGAAAGATTGGATCTTGAGTCGAGTCCACAAAAATGGGAGAAAACCCGACAATATCATCCGTGCACTCCGAGAACATGGTAAGTCATCTACAAACATTCCAAAATTTGAATTAGGATTTGATATGGAAAGCTATCCTGACCTACCAACGTGGTAGGAATAGTTTTCTCTCTTAGAAAGCAGGTGACACGATTAATGGTTCACCATTTCTTATCATTTGAGGTCGGATTTGCAGTGAAATTAGGAGCGAAGATCACAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATTGTAAGGGTTGGAGGGGTGAGGAAGATCTACAAGAAGCTATTCACAGTGAGTGAAGGAGAGAAGCTTTTGAAGGCATCACAATGCTATTTGTCAACAACAGCTGGGCCTATGGCTGGCCTTCTCTTCATTTCCACTCATAACATTGCGTTTTGCAGTGACAAATCCATCAAACTCTCTTCCCCAAATGGCGAAGAACATGTTAGAATTCACTATAAGGTAATCAAGCCCTCCTTAAGAACGCCTGTTTTTGATTAATTTGTGATTTGTAAGCTTAAGATGAACAAATTTTGCAGGTTGTGATACCAAATGAGAAGGTGATGAGAGTAAATGAGAGTGAGAATGTGAAGAAAGCATCACAAAAGTACATACAGATAGTGACAGTGGACAAGTTCGAGTTTTGGTTTATGGGGTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAAGGACTACAAAGATGA

mRNA sequence

ATGAAAATTACAATTCAAGAACATGTTTTTGGAGCTCAGATGAAGTCTTTGGGTCTTGCTCGAGCAAGATTTTTGGCTGATCCCTTCAAGCATCTCCATATGTCTTCTTTAGATGATGGGTCTCAATCAATTAAGAAATATAATGGGAAAGATTGGATCTTGAGTCGAGTCCACAAAAATGGGAGAAAACCCGACAATATCATCCGTGCACTCCGAGAACATGGAATAGTTTTCTCTCTTAGAAAGCAGGTGACACGATTAATGGTTCACCATTTCTTATCATTTGAGGTCGGATTTGCAGTGAAATTAGGAGCGAAGATCACAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATTGTAAGGGTTGGAGGGGTGAGGAAGATCTACAAGAAGCTATTCACAGTGAGTGAAGGAGAGAAGCTTTTGAAGGCATCACAATGCTATTTGTCAACAACAGCTGGGCCTATGGCTGGCCTTCTCTTCATTTCCACTCATAACATTGCGTTTTGCAGTGACAAATCCATCAAACTCTCTTCCCCAAATGGCGAAGAACATGTTAGAATTCACTATAAGGTTGTGATACCAAATGAGAAGGTGATGAGAGTAAATGAGAGTGAGAATGTGAAGAAAGCATCACAAAAGTACATACAGATAGTGACAGTGGACAAGTTCGAGTTTTGGTTTATGGGGTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAAGGACTACAAAGATGA

Coding sequence (CDS)

ATGAAAATTACAATTCAAGAACATGTTTTTGGAGCTCAGATGAAGTCTTTGGGTCTTGCTCGAGCAAGATTTTTGGCTGATCCCTTCAAGCATCTCCATATGTCTTCTTTAGATGATGGGTCTCAATCAATTAAGAAATATAATGGGAAAGATTGGATCTTGAGTCGAGTCCACAAAAATGGGAGAAAACCCGACAATATCATCCGTGCACTCCGAGAACATGGAATAGTTTTCTCTCTTAGAAAGCAGGTGACACGATTAATGGTTCACCATTTCTTATCATTTGAGGTCGGATTTGCAGTGAAATTAGGAGCGAAGATCACAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATTGTAAGGGTTGGAGGGGTGAGGAAGATCTACAAGAAGCTATTCACAGTGAGTGAAGGAGAGAAGCTTTTGAAGGCATCACAATGCTATTTGTCAACAACAGCTGGGCCTATGGCTGGCCTTCTCTTCATTTCCACTCATAACATTGCGTTTTGCAGTGACAAATCCATCAAACTCTCTTCCCCAAATGGCGAAGAACATGTTAGAATTCACTATAAGGTTGTGATACCAAATGAGAAGGTGATGAGAGTAAATGAGAGTGAGAATGTGAAGAAAGCATCACAAAAGTACATACAGATAGTGACAGTGGACAAGTTCGAGTTTTGGTTTATGGGGTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAAGGACTACAAAGATGA

Protein sequence

MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKNGRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQGLQR
Homology
BLAST of Csor.00g219730 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-47
Identity = 94/143 (65.73%), Postives = 119/143 (83.22%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           K+  K+TETVK KLSLGARI++VGG+ KI+K+LF VSEGEKL K  QCYLSTTAGP+AGL
Sbjct: 68  KIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGL 127

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  +AFCS++SIK+ SP G + +R+HYKV IP  K+ RVN+S+N KK SQKY+++V
Sbjct: 128 LFISSKKMAFCSERSIKVDSPQG-DIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVV 187

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD F+FWFMGFL+YQ  FN L+
Sbjct: 188 TVDGFDFWFMGFLSYQKAFNCLE 209

BLAST of Csor.00g219730 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-47
Identity = 95/143 (66.43%), Postives = 119/143 (83.22%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGARI+++GG+ KIYK+LF VS+ EKL KA QCYLSTTAGP+AGL
Sbjct: 69  KLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGL 128

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP GE + R+HYKV IP  K+  VN+S+N  K SQKY+++V
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGELN-RVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVV 188

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD F+FWFMGFL+YQ  FN L+
Sbjct: 189 TVDGFDFWFMGFLSYQKAFNCLE 210

BLAST of Csor.00g219730 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 5.0e-46
Identity = 92/143 (64.34%), Postives = 117/143 (81.82%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGA+I+++GG+ KIYK+LF V + EKL KA QCYLSTTAGP+AGL
Sbjct: 69  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGL 128

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP G    R+HYKV IP  K+  VN+S+N KK SQKY++IV
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGVLS-RVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIV 188

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           T+D F+FWFMGF++YQ  FN L+
Sbjct: 189 TIDNFDFWFMGFVSYQKAFNCLE 210

BLAST of Csor.00g219730 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.1e-44
Identity = 88/143 (61.54%), Postives = 116/143 (81.12%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGA+I+++GG+ KIYK+LF V + EKL KA QCYLSTT G +AGL
Sbjct: 61  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGL 120

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP G +  R+HYKV IP  K+  VN+S+N KK SQ+Y+++V
Sbjct: 121 LFISSKKIAFCSERSIKVTSPQG-DLTRVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVV 180

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD ++FWFMGF++YQ  FN L+
Sbjct: 181 TVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Csor.00g219730 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-27
Identity = 61/143 (42.66%), Postives = 93/143 (65.03%), Query Frame = 0

Query: 101 VKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAG 160
           +K    I++    +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G
Sbjct: 78  LKFSPSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTG 137

Query: 161 LLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQI 220
           +++IS   IAFCSD +I+L S  G   V  +YKVV+  EK+  ++ S NV K S++Y+ +
Sbjct: 138 VIYISNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHM 197

Query: 221 VTVDKFEFWFMGFLNYQSTFNSL 244
           VT D FEFWFMGF++Y   FN L
Sbjct: 198 VTRDGFEFWFMGFVSYIDAFNCL 220

BLAST of Csor.00g219730 vs. NCBI nr
Match: KAG6601070.1 (GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493 bits (1268), Expect = 1.32e-175
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240

Query: 241 NSLQGLQR 248
           NSLQGLQR
Sbjct: 241 NSLQGLQR 248

BLAST of Csor.00g219730 vs. NCBI nr
Match: XP_022999500.1 (putative GEM-like protein 8 [Cucurbita maxima])

HSP 1 Score: 416 bits (1070), Expect = 7.82e-146
Identity = 217/248 (87.50%), Postives = 218/248 (87.90%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHVFGAQMKSLGLARARFLADP KHLHMSSLDDG QSIKKYNGKDWIL+RVHKN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPSKHLHMSSLDDGYQSIKKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTT GPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 222

Query: 241 NSLQGLQR 248
           NSLQ LQR
Sbjct: 241 NSLQELQR 222

BLAST of Csor.00g219730 vs. NCBI nr
Match: XP_022956364.1 (GEM-like protein 4 [Cucurbita moschata])

HSP 1 Score: 414 bits (1063), Expect = 9.12e-145
Identity = 215/248 (86.69%), Postives = 217/248 (87.50%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHVFGAQMKSLGLARARFLADP KH HMSSLDDGSQSI+KYNGKDWIL+RVHKN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKL LGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLRLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFL YQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTF 222

Query: 241 NSLQGLQR 248
           NSLQ LQR
Sbjct: 241 NSLQALQR 222

BLAST of Csor.00g219730 vs. NCBI nr
Match: XP_023513129.1 (putative GEM-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409 bits (1050), Expect = 9.05e-143
Identity = 213/248 (85.89%), Postives = 215/248 (86.69%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHV GAQMKSLGLARARFLADP KHLHMSS DDG QSIKKYNGKDWIL+RVHKN
Sbjct: 1   MKITIQEHVLGAQMKSLGLARARFLADPSKHLHMSSSDDGYQSIKKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIK S
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKFS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVD FEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDNFEFWFMGFLNYQSTF 222

Query: 241 NSLQGLQR 248
           NSLQ LQ+
Sbjct: 241 NSLQELQK 222

BLAST of Csor.00g219730 vs. NCBI nr
Match: KAG6601069.1 (GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398 bits (1022), Expect = 1.73e-138
Identity = 210/248 (84.68%), Postives = 213/248 (85.89%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MK  IQ+ V G Q+ SLGLARARFLADP KHLHMSSLDDGSQSIKKYNGKDWILSRVHKN
Sbjct: 1   MKSAIQQQVLGMQL-SLGLARARFLADPSKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 221

Query: 241 NSLQGLQR 248
           NSLQ L +
Sbjct: 241 NSLQELHK 221

BLAST of Csor.00g219730 vs. ExPASy TrEMBL
Match: A0A6J1KFK0 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 3.79e-146
Identity = 217/248 (87.50%), Postives = 218/248 (87.90%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHVFGAQMKSLGLARARFLADP KHLHMSSLDDG QSIKKYNGKDWIL+RVHKN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPSKHLHMSSLDDGYQSIKKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTT GPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 222

Query: 241 NSLQGLQR 248
           NSLQ LQR
Sbjct: 241 NSLQELQR 222

BLAST of Csor.00g219730 vs. ExPASy TrEMBL
Match: A0A6J1GYU0 (GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458125 PE=3 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 4.42e-145
Identity = 215/248 (86.69%), Postives = 217/248 (87.50%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MKITIQEHVFGAQMKSLGLARARFLADP KH HMSSLDDGSQSI+KYNGKDWIL+RVHKN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKL LGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLRLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFL YQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTF 222

Query: 241 NSLQGLQR 248
           NSLQ LQR
Sbjct: 241 NSLQALQR 222

BLAST of Csor.00g219730 vs. ExPASy TrEMBL
Match: A0A6J1GXL4 (GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458126 PE=3 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 6.18e-138
Identity = 208/248 (83.87%), Postives = 212/248 (85.48%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MK  IQ+ V G Q+ SLGLARARFLADP KH HMSSLDDGSQSI+KYNGKDWIL+RVHKN
Sbjct: 1   MKSAIQQQVLGMQL-SLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIRALREH                          VKLGAKITETVKGKL LGAR
Sbjct: 61  GRKPDNIIRALREH--------------------------VKLGAKITETVKGKLRLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFL YQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTF 221

Query: 241 NSLQGLQR 248
           NSLQGLQR
Sbjct: 241 NSLQGLQR 221

BLAST of Csor.00g219730 vs. ExPASy TrEMBL
Match: A0A6J1KB26 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=1)

HSP 1 Score: 393 bits (1009), Expect = 7.21e-137
Identity = 207/248 (83.47%), Postives = 211/248 (85.08%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MK  IQ+HV G Q+ SL LARARFLA P KHLHMSSLDDGSQSI+KYNGKDWIL+RVHKN
Sbjct: 1   MKSAIQQHVLGMQL-SLALARARFLAHPSKHLHMSSLDDGSQSIQKYNGKDWILNRVHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRKPDNIIR LREH                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKPDNIIRELREH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTT GPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF
Sbjct: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 221

Query: 241 NSLQGLQR 248
           NSLQ LQR
Sbjct: 241 NSLQELQR 221

BLAST of Csor.00g219730 vs. ExPASy TrEMBL
Match: A0A6J1GW39 (putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111458124 PE=3 SV=1)

HSP 1 Score: 363 bits (932), Expect = 4.29e-125
Identity = 190/244 (77.87%), Postives = 198/244 (81.15%), Query Frame = 0

Query: 1   MKITIQEHVFGAQMKSLGLARARFLADPFKHLHMSSLDDGSQSIKKYNGKDWILSRVHKN 60
           MK TIQE V GAQMKSL LARARFLADP KHLH+SS DDGSQSIKKYNGKDWIL+R+HKN
Sbjct: 1   MKTTIQEQVLGAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWILNRLHKN 60

Query: 61  GRKPDNIIRALREHGIVFSLRKQVTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGAR 120
           GRK DNII ALR+H                          VKLGAKITETVKGKLSLGAR
Sbjct: 61  GRKTDNIIHALRQH--------------------------VKLGAKITETVKGKLSLGAR 120

Query: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180
           IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS
Sbjct: 121 IVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLS 180

Query: 181 SPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTF 240
           SPNGEEHVRIHYKV IP  K+ RV ESENVK   +KYI++VTVD FEFWFMGFLNY  TF
Sbjct: 181 SPNGEEHVRIHYKVTIPLGKIRRVFESENVKNPWEKYIEMVTVDDFEFWFMGFLNYHKTF 218

Query: 241 NSLQ 244
           N LQ
Sbjct: 241 NCLQ 218

BLAST of Csor.00g219730 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 191.4 bits (485), Expect = 8.5e-49
Identity = 94/143 (65.73%), Postives = 119/143 (83.22%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           K+  K+TETVK KLSLGARI++VGG+ KI+K+LF VSEGEKL K  QCYLSTTAGP+AGL
Sbjct: 68  KIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGL 127

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  +AFCS++SIK+ SP G + +R+HYKV IP  K+ RVN+S+N KK SQKY+++V
Sbjct: 128 LFISSKKMAFCSERSIKVDSPQG-DIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVV 187

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD F+FWFMGFL+YQ  FN L+
Sbjct: 188 TVDGFDFWFMGFLSYQKAFNCLE 209

BLAST of Csor.00g219730 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 190.7 bits (483), Expect = 1.4e-48
Identity = 95/143 (66.43%), Postives = 119/143 (83.22%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGARI+++GG+ KIYK+LF VS+ EKL KA QCYLSTTAGP+AGL
Sbjct: 69  KLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGL 128

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP GE + R+HYKV IP  K+  VN+S+N  K SQKY+++V
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGELN-RVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVV 188

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD F+FWFMGFL+YQ  FN L+
Sbjct: 189 TVDGFDFWFMGFLSYQKAFNCLE 210

BLAST of Csor.00g219730 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 186.0 bits (471), Expect = 3.6e-47
Identity = 92/143 (64.34%), Postives = 117/143 (81.82%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGA+I+++GG+ KIYK+LF V + EKL KA QCYLSTTAGP+AGL
Sbjct: 131 KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGL 190

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP G    R+HYKV IP  K+  VN+S+N KK SQKY++IV
Sbjct: 191 LFISSKKIAFCSERSIKVASPQGVLS-RVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIV 250

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           T+D F+FWFMGF++YQ  FN L+
Sbjct: 251 TIDNFDFWFMGFVSYQKAFNCLE 272

BLAST of Csor.00g219730 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 180.6 bits (457), Expect = 1.5e-45
Identity = 88/143 (61.54%), Postives = 116/143 (81.12%), Query Frame = 0

Query: 102 KLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAGL 161
           KLG K+TETVK KLSLGA+I+++GG+ KIYK+LF V + EKL KA QCYLSTT G +AGL
Sbjct: 61  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGL 120

Query: 162 LFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQIV 221
           LFIS+  IAFCS++SIK++SP G +  R+HYKV IP  K+  VN+S+N KK SQ+Y+++V
Sbjct: 121 LFISSKKIAFCSERSIKVTSPQG-DLTRVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVV 180

Query: 222 TVDKFEFWFMGFLNYQSTFNSLQ 245
           TVD ++FWFMGF++YQ  FN L+
Sbjct: 181 TVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Csor.00g219730 vs. TAIR 10
Match: AT4G01600.2 (GRAM domain family protein )

HSP 1 Score: 126.3 bits (316), Expect = 3.4e-29
Identity = 62/143 (43.36%), Postives = 93/143 (65.03%), Query Frame = 0

Query: 101 VKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTAGPMAG 160
           VK    I++    +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G
Sbjct: 73  VKFSPSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTG 132

Query: 161 LLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYIQI 220
           +++IS   IAFCSD +I+L S  G   V  +YKVV+  EK+  ++ S NV K S++Y+ +
Sbjct: 133 VIYISNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHM 192

Query: 221 VTVDKFEFWFMGFLNYQSTFNSL 244
           VT D FEFWFMGF++Y   FN L
Sbjct: 193 VTRDGFEFWFMGFVSYIDAFNCL 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FTA01.2e-4765.73GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW42.0e-4766.43Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FMW65.0e-4664.34GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FMW52.1e-4461.54GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9M1221.1e-2742.66GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6601070.11.32e-175100.00GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022999500.17.82e-14687.50putative GEM-like protein 8 [Cucurbita maxima][more]
XP_022956364.19.12e-14586.69GEM-like protein 4 [Cucurbita moschata][more]
XP_023513129.19.05e-14385.89putative GEM-like protein 8 [Cucurbita pepo subsp. pepo][more]
KAG6601069.11.73e-13884.68GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1KFK03.79e-14687.50putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=... [more]
A0A6J1GYU04.42e-14586.69GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458125 PE=3 SV=1[more]
A0A6J1GXL46.18e-13883.87GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458126 PE=3 SV=1[more]
A0A6J1KB267.21e-13783.47putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=... [more]
A0A6J1GW394.29e-12577.87putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111458124 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G08350.18.5e-4965.73GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23370.11.4e-4866.43GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.13.6e-4764.34GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.11.5e-4561.54GRAM domain-containing protein / ABA-responsive protein-related [more]
AT4G01600.23.4e-2943.36GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 129..208
e-value: 3.8E-16
score: 69.6
IPR004182GRAM domainPFAMPF02893GRAMcoord: 132..246
e-value: 4.0E-18
score: 65.3
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 127..248
e-value: 2.0E-20
score: 74.6
NoneNo IPR availablePANTHERPTHR31969:SF38GEM-LIKE PROTEIN 4coord: 96..244
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 96..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g219730.m01Csor.00g219730.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity