Csor.00g217590 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g217590
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description4-coumarate--CoA ligase
LocationCsor_Chr10: 1960298 .. 1963440 (+)
RNA-Seq ExpressionCsor.00g217590
SyntenyCsor.00g217590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTGCTGATTCCTCGCCGGAATTCATATTCCGGTCAAAGCTCCCGGACCTCACCATCCCCAACCACCTTCCCTTGCATACATATTGCTTCGAAAGAATCTCCAAATTCAAACACAATCCATGTCTAATCAATGCTGCCACCGGTGACATTTACACGTACGGTGAAGTCCACGTAACGGCCCGCCGAGTAGCCGCCGGCTTTCATAAGCTCGGCATCGGAAAAGGGGACGTCATCATGCTCCTTCTCCAAAACACTCCACAATTCGTTTTCGCTTTCCTCGGCGCTTCCTTTGCGTGCGCCATCGCCACCATGGCGAACCCGTTCTTCACGCCAGCGGAGATTGCGAAGCAAGCGGCGTCGTCCGGCGCTAAGCTGATCATAACCCAAGCCGCGTTCGCGGAAAAAGTGAAAAAGCTGCCTCAGGAAAACCAGGGGATTTTGAAAGTGATATTCATCGACTCTCCACCGGAGGGTGGATGTCATTTTACGTCGTTGACTGATGAGGCAAAAGAGGAGGAAATGGCGGATGTGAAATTCAGCCCGGACGACGTCGTTGCGTTGCCATATTCGTCTGGCACGACAGGCCTCCCAAAAGGAGTTATGCTGACTCATAAGGGATTAGTGACCAGCGTGGCTCAACAAGTGGACGGCGAAAATCCACACTTCCATATCCGAAGTAACGACGTCGTTCTGTGTGTGCTTCCTTTGTTTCACATCTACTCATTGAGCTCGATCGTGATGACGTCTTTGCGAGTGGGAGCTGCGATTTTAATCGTCCAGAAATTCGACATTAATTCCCTTGTGGCATTGGTTCCCAAATATAGGGTCACCATTGCTACCGTTGTACCTCCAATTGTACTCGCAATTGTCAAGAGTCCGGTTGTAGACCAATCCGACATGTCGTCCATACGGATCGTGTTGTCGGGAGCCGCACCGCTGGGGAAGAATCTTGAAGATGCCTTTAGAGCCAAGCTTCCCCATGCCATTCTCGGCCAAGTAAATAACTAATATAATCCATTATAGTTTTGTACAAAACTGTCAATTTCATCGTTACAAAATGAAGTTTTAAAATAATTATAGGGATACGGAATGACTGAGTCAGGATCAGTGTTAACAATGTCATTAGCACTTGCAAGGGAGAAATTTGCAATCAAGTCGGGAGCTTGTGGGACATTAATGAGAAATGCAGAGATGAAGATTGTAAACCCCGAGACCGGAGTTTCTCTTTCAAGGAATCAACCGGGAGAGATTTATGTTAGAAGTCCTCAAATGATGAAAGGTATGCGAACATTATTCTTGTGAAATTCATACCTTAACTCACACCATTAAGTTTGATATATATAATATGTTAATATTCGTTAATAAAAAGGGTTTATAATGTATATGACACAAAGGAGGTAGTGGCAGCTGATGTAAGTAATTAATGCCGCCTAACCATTCCACAAGAAAAAAACGTTACTTATTAATACCGCCTGGGTAAGGACGTCATTTCATTTGTTTGGATTTGGAAGCGTCAATATGGGCCCACCTCACAGTCATTGGGCCTAATGGGCTCATTGTAATGACCAGACCAAGCCCGATAAAATCGAGAGAGCCCATTACATTAGTGTGTTCTGTTTCACCGTCTGAGTTTTGATTGGTTGAACTCAACACGAACCAGGGTACCTCAACGACGAAGAGGCCACGAAGGGTATAATCGACGAAGACGGATGGCTGCACACCGGCGACATGGGTTTAGTTGACGACGACGATGAGGTCTTCATCGTCGATCGTCTCAAGGAACTGATCAAATACAAAGGCTTCCAGGTAGCGCCGGCGGAGTTGGAAGGCCTGCTCATTTCCAACGTCCACATTGTCGACGTTGCGGTTATACCGTATGTCAGCATTCTCGATTCTGCTCCTTCACCCTCAAACTTCTCGGTTTAATTACTCTCTACTTTTTTTAATTCAGTATGAAAGATGAAGTAGCTGGAGAGGTTCCTGTTGCGTTCATTGTCCGGTCAGATGGTTCCAACATCACCGAGGATGAAATTAAGCAATACATCTCCAAACAGGTGAAGTCTCCAATTTTGGCCTTATTTTCAAGTTTTTAAGTTTGATATCTTATTAGCTTTTATCCATTGATGGATAGCGTCGATTTCACCGAATAATTAGGTAACCTTGCGTTTATGATATAACTCATCGTAGGCCCCTGCTCTTTCGTTTCCCTTCCTATTTGGCTATAATTCCTTAACCTTTAATGTCTATGCCTACCTTAAAAAAAAAAAAAAAAAGAGTTTATGTCTATTGGATCAATACCCTTTCAAAAATTCTAATAGCTGGTTGAACTTTTAATATTGTGATTTGGGTTCATGTAGGCCAATGTGATCCCACGAGGGAACATTCTTTAGAAGGGTATGGAAACCTTTTACGAGTAGAAGATCAAAAGGGAAAATAAGAAAAATAATATCTATTAGCTCTGTGCTTGAGCCATTACAAATAGTATCAGAATTAAACACCGGGCGGTGTGCCGAGGATGCTAGGCTTACAAAGGAGGTTGATTGTGAGATCCCAAATTGGTTGGAGAGAGGGAATGAAACTTTCCTTTCAAGGGTGTGGAAACCTCTTTCCGATAGACACGTTTCAAAACCTTGAGGGAAAACTCGAAAGGAAAAGTTCAAAAAAATGATAATATCTGCTAGCCGTGGGTTTGAGCTATTACAAATGGCATAGAGTCAGTCACCAGACAAGGTGCAGTGAGGATGCTGGTCCCCAAGGGAGTTAGATTGTAAGATCTCACATCGGTTGGAGAGGGAAATGTTTCTTATAAGGGTGTAGAAACGTCTCTCCAGTAGAGATATTTTAAAACCTTGAGCGGAAGCCATTACACACAATATTAGATATGTATGGAAAACCTAAGAGATTTTTTATGCTTATAAGTTCAAAAGCCTAATAAAGCCTAACTTAATAGTTTAATCTTAATTGAAATCTCGGAGATGTGATACATGGATTGTAACTATTGAGGACGTGGGATGCAGGTTGTGTTTTATAAGAGGATTAATCGCGTCTTCTTCGTGGATTCCATCCCTAAAAGTCCTTCTGGAAAAATCTTGCGGAGACAACTTAGAGCTCTGCTCCCCGCCAATATTCCAAACTAG

mRNA sequence

ATGGCGGCTGCTGATTCCTCGCCGGAATTCATATTCCGGTCAAAGCTCCCGGACCTCACCATCCCCAACCACCTTCCCTTGCATACATATTGCTTCGAAAGAATCTCCAAATTCAAACACAATCCATGTCTAATCAATGCTGCCACCGGTGACATTTACACGTACGGTGAAGTCCACGTAACGGCCCGCCGAGTAGCCGCCGGCTTTCATAAGCTCGGCATCGGAAAAGGGGACGTCATCATGCTCCTTCTCCAAAACACTCCACAATTCGTTTTCGCTTTCCTCGGCGCTTCCTTTGCGTGCGCCATCGCCACCATGGCGAACCCGTTCTTCACGCCAGCGGAGATTGCGAAGCAAGCGGCGTCGTCCGGCGCTAAGCTGATCATAACCCAAGCCGCGTTCGCGGAAAAAGTGAAAAAGCTGCCTCAGGAAAACCAGGGGATTTTGAAAGTGATATTCATCGACTCTCCACCGGAGGGTGGATGTCATTTTACGTCGTTGACTGATGAGGCAAAAGAGGAGGAAATGGCGGATGTGAAATTCAGCCCGGACGACGTCGTTGCGTTGCCATATTCGTCTGGCACGACAGGCCTCCCAAAAGGAGTTATGCTGACTCATAAGGGATTAGTGACCAGCGTGGCTCAACAAGTGGACGGCGAAAATCCACACTTCCATATCCGAAGTAACGACGTCGTTCTGTGTGTGCTTCCTTTGTTTCACATCTACTCATTGAGCTCGATCGTGATGACGTCTTTGCGAGTGGGAGCTGCGATTTTAATCGTCCAGAAATTCGACATTAATTCCCTTGTGGCATTGGTTCCCAAATATAGGGTCACCATTGCTACCGTTGTACCTCCAATTGTACTCGCAATTGTCAAGAGTCCGGTTGTAGACCAATCCGACATGTCGTCCATACGGATCGTGTTGTCGGGAGCCGCACCGCTGGGGAAGAATCTTGAAGATGCCTTTAGAGCCAAGCTTCCCCATGCCATTCTCGGCCAAGGATACGGAATGACTGAGTCAGGATCAGTGTTAACAATGTCATTAGCACTTGCAAGGGAGAAATTTGCAATCAAGTCGGGAGCTTGTGGGACATTAATGAGAAATGCAGAGATGAAGATTGTAAACCCCGAGACCGGAGTTTCTCTTTCAAGGAATCAACCGGGAGAGATTTATGTTAGAAGTCCTCAAATGATGAAAGGGTACCTCAACGACGAAGAGGCCACGAAGGGTATAATCGACGAAGACGGATGGCTGCACACCGGCGACATGGGTTTAGTTGACGACGACGATGAGGTCTTCATCGTCGATCGTCTCAAGGAACTGATCAAATACAAAGGCTTCCAGGTAGCGCCGGCGGAGTTGGAAGGCCTGCTCATTTCCAACGTCCACATTGTCGACGTTGCGGTTATACCTATGAAAGATGAAGTAGCTGGAGAGGTTCCTGTTGCGTTCATTGTCCGGTCAGATGGTTCCAACATCACCGAGGATGAAATTAAGCAATACATCTCCAAACAGGTTGTGTTTTATAAGAGGATTAATCGCGTCTTCTTCGTGGATTCCATCCCTAAAAGTCCTTCTGGAAAAATCTTGCGGAGACAACTTAGAGCTCTGCTCCCCGCCAATATTCCAAACTAG

Coding sequence (CDS)

ATGGCGGCTGCTGATTCCTCGCCGGAATTCATATTCCGGTCAAAGCTCCCGGACCTCACCATCCCCAACCACCTTCCCTTGCATACATATTGCTTCGAAAGAATCTCCAAATTCAAACACAATCCATGTCTAATCAATGCTGCCACCGGTGACATTTACACGTACGGTGAAGTCCACGTAACGGCCCGCCGAGTAGCCGCCGGCTTTCATAAGCTCGGCATCGGAAAAGGGGACGTCATCATGCTCCTTCTCCAAAACACTCCACAATTCGTTTTCGCTTTCCTCGGCGCTTCCTTTGCGTGCGCCATCGCCACCATGGCGAACCCGTTCTTCACGCCAGCGGAGATTGCGAAGCAAGCGGCGTCGTCCGGCGCTAAGCTGATCATAACCCAAGCCGCGTTCGCGGAAAAAGTGAAAAAGCTGCCTCAGGAAAACCAGGGGATTTTGAAAGTGATATTCATCGACTCTCCACCGGAGGGTGGATGTCATTTTACGTCGTTGACTGATGAGGCAAAAGAGGAGGAAATGGCGGATGTGAAATTCAGCCCGGACGACGTCGTTGCGTTGCCATATTCGTCTGGCACGACAGGCCTCCCAAAAGGAGTTATGCTGACTCATAAGGGATTAGTGACCAGCGTGGCTCAACAAGTGGACGGCGAAAATCCACACTTCCATATCCGAAGTAACGACGTCGTTCTGTGTGTGCTTCCTTTGTTTCACATCTACTCATTGAGCTCGATCGTGATGACGTCTTTGCGAGTGGGAGCTGCGATTTTAATCGTCCAGAAATTCGACATTAATTCCCTTGTGGCATTGGTTCCCAAATATAGGGTCACCATTGCTACCGTTGTACCTCCAATTGTACTCGCAATTGTCAAGAGTCCGGTTGTAGACCAATCCGACATGTCGTCCATACGGATCGTGTTGTCGGGAGCCGCACCGCTGGGGAAGAATCTTGAAGATGCCTTTAGAGCCAAGCTTCCCCATGCCATTCTCGGCCAAGGATACGGAATGACTGAGTCAGGATCAGTGTTAACAATGTCATTAGCACTTGCAAGGGAGAAATTTGCAATCAAGTCGGGAGCTTGTGGGACATTAATGAGAAATGCAGAGATGAAGATTGTAAACCCCGAGACCGGAGTTTCTCTTTCAAGGAATCAACCGGGAGAGATTTATGTTAGAAGTCCTCAAATGATGAAAGGGTACCTCAACGACGAAGAGGCCACGAAGGGTATAATCGACGAAGACGGATGGCTGCACACCGGCGACATGGGTTTAGTTGACGACGACGATGAGGTCTTCATCGTCGATCGTCTCAAGGAACTGATCAAATACAAAGGCTTCCAGGTAGCGCCGGCGGAGTTGGAAGGCCTGCTCATTTCCAACGTCCACATTGTCGACGTTGCGGTTATACCTATGAAAGATGAAGTAGCTGGAGAGGTTCCTGTTGCGTTCATTGTCCGGTCAGATGGTTCCAACATCACCGAGGATGAAATTAAGCAATACATCTCCAAACAGGTTGTGTTTTATAAGAGGATTAATCGCGTCTTCTTCGTGGATTCCATCCCTAAAAGTCCTTCTGGAAAAATCTTGCGGAGACAACTTAGAGCTCTGCTCCCCGCCAATATTCCAAACTAG

Protein sequence

MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLPANIPN
Homology
BLAST of Csor.00g217590 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 9.2e-210
Identity = 370/537 (68.90%), Postives = 446/537 (83.05%), Query Frame = 0

Query: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARR 64
           D   EFIFRSKLPD+ IPNHLPLH+YCFE IS+FK  PCLIN ATG+I TY +V +T+R+
Sbjct: 8   DHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSRK 67

Query: 65  VAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSG 124
           VAAG  KLGI +GDVIMLLLQN+P+FV+AFL AS+  AI T ANPF+TPAE+AKQAA+S 
Sbjct: 68  VAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASK 127

Query: 125 AKLIITQAAFAEKVKKLPQENQGILKVIFIDS-PPEGGC-HFTSLTDEAKEEEMADVKFS 184
            KL+IT A + +KVK+      G+ KV+ +D+ PPE  C HF+ LT +A E E+  VK  
Sbjct: 128 TKLVITLAGYIDKVKEFTGGESGV-KVMCVDAPPPESECLHFSELT-QADETEIPAVKIH 187

Query: 185 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIY 244
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NP+ +   NDV+LCVLPLFHIY
Sbjct: 188 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIY 247

Query: 245 SLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDM 304
           SL+SI++  LRVGAAILI+QKF+I+ L+ L+ K++VTIA  VPPIVL++ K P + + D+
Sbjct: 248 SLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDL 307

Query: 305 SSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGA 364
           SSIR V+SG AP+GK LEDA + KLPHA LGQGYGMTE+G VL+M LA A+E F IKSGA
Sbjct: 308 SSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGA 367

Query: 365 CGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTG 424
           CGT++RNAEMKIV+P+TG SL RNQ GEI +R  Q+MKGY+ND EATKG IDE GWLHTG
Sbjct: 368 CGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTG 427

Query: 425 DMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVP 484
           D+G +D+DDE+FIVDRLKELIKYKGFQVAPAELE +LIS+ +I D AV+PMKDE AGEVP
Sbjct: 428 DIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVP 487

Query: 485 VAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALL 540
           VAF+VRS+GS ITE++IKQYISKQVVFYKRIN+ FF++ IPK+PSGKILR+ LRA L
Sbjct: 488 VAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

BLAST of Csor.00g217590 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-209
Identity = 367/539 (68.09%), Postives = 440/539 (81.63%), Query Frame = 0

Query: 7   SPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVA 66
           S + IFRSKLPD+ IP HLPLH+YCFE IS+F   PCLIN A   IYTY EV +T R+VA
Sbjct: 12  SGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVA 71

Query: 67  AGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAK 126
            G +KLGI + D IM+LL N+P+FVFAF+GAS+  AI+TMANP FTPAE+ KQA +S AK
Sbjct: 72  VGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 127 LIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVKFSPDDV 186
           +IITQ+ F  KVK    EN   +KVI IDS PEG  HF+ LT ++ E E+ +VK  PDDV
Sbjct: 132 IIITQSCFVGKVKDYASEND--VKVICIDSAPEGCLHFSELT-QSDEHEIPEVKIQPDDV 191

Query: 187 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSLSS 246
           VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN + ++ S DV++CVLPLFHIYSL+S
Sbjct: 192 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNS 251

Query: 247 IVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSSIR 306
           I++  LRVGAAILI+QKFDI   + L+ KY+V+I   VPPIVLAI KSP+VD  D+SS+R
Sbjct: 252 ILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVR 311

Query: 307 IVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACGTL 366
            V+SGAAPLGK LEDA R K P+A LGQGYGMTE+G VL M LA A+E F IKSGACGT+
Sbjct: 312 TVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTV 371

Query: 367 MRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDMGL 426
           +RNAEMKIV+P+TG SL RNQPGEI +R  Q+MKGYLND EAT   ID++GWLHTGD+G 
Sbjct: 372 VRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGF 431

Query: 427 VDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVAFI 486
           +D+DDE+FIVDRLKELIKYKGFQVAPAE+E LL+++ +I D AV+PMKDE AGEVPVAF+
Sbjct: 432 IDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFV 491

Query: 487 VRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLPANIPN 546
           VRS+GS ITEDE+K +ISKQV+FYKR+ RVFFV+++PKSPSGKILR+ LRA L A +PN
Sbjct: 492 VRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Csor.00g217590 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 3.5e-209
Identity = 369/537 (68.72%), Postives = 439/537 (81.75%), Query Frame = 0

Query: 9   EFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVAAG 68
           + IFRSKLPD+ IPNHLPLH+YCFE IS+F   PCLIN A   IYTY +V + +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 69  FHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAKLI 128
            HK GI   D IM+LL N+P+FVFAF+GAS+  AI+TMANP FTPAE+ KQA +S AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 129 ITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVKFSPDDVVA 188
           +TQA    KVK    EN   +K+I IDS PEG  HF+ LT +A E ++ +V+  PDDVVA
Sbjct: 129 VTQACHVNKVKDYAFEND--VKIICIDSAPEGCLHFSVLT-QANEHDIPEVEIQPDDVVA 188

Query: 189 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSLSSIV 248
           LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+ +I S DV+LCVLPLFHIYSL+S++
Sbjct: 189 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVL 248

Query: 249 MTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSSIRIV 308
           +  LRVGAAILI+QKFDI S + L+ +Y+VTI   VPPIVLAI KSP+VD  D+SS+R V
Sbjct: 249 LCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTV 308

Query: 309 LSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACGTLMR 368
           +SGAAPLGK LED  RAK P+A LGQGYGMTE+G VL M LA A+E F IKSGACGT++R
Sbjct: 309 MSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVR 368

Query: 369 NAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDMGLVD 428
           NAEMKIV+P+TG SL RNQ GEI +R  Q+MKGYLND EAT   ID++GWL+TGD+G +D
Sbjct: 369 NAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYID 428

Query: 429 DDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVAFIVR 488
           DDDE+FIVDRLKELIKYKGFQVAPAELE LL+++ +I D AV+PMKDE AGEVPVAF+VR
Sbjct: 429 DDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVR 488

Query: 489 SDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLPANIPN 546
           S+GS ITEDE+K +ISKQV+FYKRI RVFFVD+IPKSPSGKILR+ LRA L A +PN
Sbjct: 489 SNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Csor.00g217590 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 1.0e-208
Identity = 369/545 (67.71%), Postives = 440/545 (80.73%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           M    +  + IFRSKLPD+ IP HLPLH+YCFE IS+F   PCLIN A   IYTY +V +
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVEL 62

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           T+R+VAAG +KLGI + D IM+LL N+P+FVFAF+GAS+  AI+TMANP FTPAE+ KQA
Sbjct: 63  TSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQA 122

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
            +S AKLIITQA F  KVK    +N   L VI IDS PEG  HF+ LT +A E ++ DVK
Sbjct: 123 KASNAKLIITQACFVNKVKDYAFDNN--LNVICIDSAPEGCIHFSELT-QADEHDIPDVK 182

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
              DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN + ++ S DV++CVLPLFH
Sbjct: 183 IQSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFH 242

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSL+S+++  LRVGAAILI+QKFDI     L+ KY+VTI   VPPIVLAI KSPVVD  
Sbjct: 243 IYSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNY 302

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           D+SS+R V+SGAAPLGK LEDA R K P+A LGQGYGMTE+G VL M LA A+E F IKS
Sbjct: 303 DLSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 362

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGT++RNAEMKIV+P+TG SL RNQPGEI +R  Q+MKGYLND  AT   ID++GWLH
Sbjct: 363 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLH 422

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGD+G +D+DDE+FIVDRLKELIKYKGFQVAPAELE LL+++ +I D AV+PMKDE AGE
Sbjct: 423 TGDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGE 482

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
           VPVAF+VRS+GS+ITEDE+K ++SKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA L 
Sbjct: 483 VPVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLA 542

Query: 541 ANIPN 546
           A +PN
Sbjct: 543 AGVPN 544

BLAST of Csor.00g217590 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 1.3e-208
Identity = 369/539 (68.46%), Postives = 441/539 (81.82%), Query Frame = 0

Query: 7   SPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVA 66
           S + IFRSKLPD+ IP HLPLH+YCFE +S+F   PCLI+ A   IYTY EV +T+R+VA
Sbjct: 10  SGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 69

Query: 67  AGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAK 126
            G +KLGI + D IM+LL N P+FVFAF+GAS+  AI+TMANP FTPAE+ KQA +S AK
Sbjct: 70  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 129

Query: 127 LIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVKFSPDDV 186
           ++ITQA FA KVK    EN   LKVI +DS PEG  HF+ L  ++ E E+ DVK  PDDV
Sbjct: 130 IVITQACFAGKVKDYAIEND--LKVICVDSVPEGCVHFSELI-QSDEHEIPDVKIQPDDV 189

Query: 187 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSLSS 246
           VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN + ++ S+DV++CVLPLFHIYSL+S
Sbjct: 190 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNS 249

Query: 247 IVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSSIR 306
           +++ +LRVGAAILI+QKFDI   + L+PK++VTI   VPPIVLAI KSP+VD  D+SS+R
Sbjct: 250 VLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR 309

Query: 307 IVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACGTL 366
            V+SGAAPLGK LEDA RAK P+A LGQGYGMTE+G VL M LA A+E F IKSGACGT+
Sbjct: 310 TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTV 369

Query: 367 MRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDMGL 426
           +RNAEMKIV+P+TG SL RNQPGEI +R  Q+MKGYLND EAT   I+++GWLHTGD+G 
Sbjct: 370 VRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGF 429

Query: 427 VDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVAFI 486
           +DDDDE+FIVDRLKELIKYKGFQVAPAELE LLI++  I D AV+PM DE AGEVPVAF+
Sbjct: 430 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFV 489

Query: 487 VRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLPANIPN 546
           VRS+GS ITEDE+K +ISKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA L A I N
Sbjct: 490 VRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGISN 545

BLAST of Csor.00g217590 vs. NCBI nr
Match: KAG6589713.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1059 bits (2739), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH
Sbjct: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
           VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           ANIPN
Sbjct: 541 ANIPN 545

BLAST of Csor.00g217590 vs. NCBI nr
Match: XP_022921335.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAIL QGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPE GVSLSRNQPGEIYVRSPQMMKGYLNDEEATKG+IDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDE+FIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
           VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           A+IPN
Sbjct: 541 ASIPN 545

BLAST of Csor.00g217590 vs. NCBI nr
Match: XP_023515445.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 528/545 (96.88%), Postives = 539/545 (98.90%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAAD SPEFIFRSKLPDLTIPNHLPLHTYCFERIS+FKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAG HKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEI KQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIGKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVK LPQENQGILKV+FIDSPPEGGCHF+SLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKMLPQENQGILKVVFIDSPPEGGCHFSSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALA+E+FAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAKERFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNP+TGVSLS+NQPGEIYV+SPQMMKGYLNDEEATKGIIDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPDTGVSLSKNQPGEIYVKSPQMMKGYLNDEEATKGIIDEHGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGD+GLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
           VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           A+IPN
Sbjct: 541 ASIPN 545

BLAST of Csor.00g217590 vs. NCBI nr
Match: XP_022987367.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1026 bits (2654), Expect = 0.0
Identity = 525/545 (96.33%), Postives = 536/545 (98.35%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAAD SPEFIFRSKLPDLTIPNHLPLHTYCFERIS+FKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAG HKLGIGKGDVIMLLLQNTPQF FAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVK LPQENQGILKVIFIDSPPEGGCHF+SLTDEAKEEE+ DVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKNLPQENQGILKVIFIDSPPEGGCHFSSLTDEAKEEELTDVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVM+SLRVGAAIL+VQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDCS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAK PHAILGQGYGMTESGSVLTMSLALA+E+FAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIID DGWLH
Sbjct: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGDGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGD+GLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
            PVAFIVRSDGSNITEDEIK+YISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 APVAFIVRSDGSNITEDEIKEYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           A+IPN
Sbjct: 541 ASIPN 545

BLAST of Csor.00g217590 vs. NCBI nr
Match: XP_022921336.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 973 bits (2515), Expect = 0.0
Identity = 500/507 (98.62%), Postives = 503/507 (99.21%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAIL QGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPE GVSLSRNQPGEIYVRSPQMMKGYLNDEEATKG+IDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDE+FIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQV 507
           VPVAFIVRSDGSNITEDEIKQYISKQV
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQV 507

BLAST of Csor.00g217590 vs. ExPASy TrEMBL
Match: A0A6J1E5C5 (4-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429639 PE=4 SV=1)

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAIL QGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPE GVSLSRNQPGEIYVRSPQMMKGYLNDEEATKG+IDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDE+FIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
           VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           A+IPN
Sbjct: 541 ASIPN 545

BLAST of Csor.00g217590 vs. ExPASy TrEMBL
Match: A0A6J1JA64 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484944 PE=4 SV=1)

HSP 1 Score: 1026 bits (2654), Expect = 0.0
Identity = 525/545 (96.33%), Postives = 536/545 (98.35%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAAD SPEFIFRSKLPDLTIPNHLPLHTYCFERIS+FKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAG HKLGIGKGDVIMLLLQNTPQF FAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVK LPQENQGILKVIFIDSPPEGGCHF+SLTDEAKEEE+ DVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKNLPQENQGILKVIFIDSPPEGGCHFSSLTDEAKEEELTDVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVM+SLRVGAAIL+VQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDCS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAK PHAILGQGYGMTESGSVLTMSLALA+E+FAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIID DGWLH
Sbjct: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGDGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGD+GLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540
            PVAFIVRSDGSNITEDEIK+YISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP
Sbjct: 481 APVAFIVRSDGSNITEDEIKEYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLP 540

Query: 541 ANIPN 545
           A+IPN
Sbjct: 541 ASIPN 545

BLAST of Csor.00g217590 vs. ExPASy TrEMBL
Match: A0A6J1E138 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429639 PE=4 SV=1)

HSP 1 Score: 973 bits (2515), Expect = 0.0
Identity = 500/507 (98.62%), Postives = 503/507 (99.21%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAIL QGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPE GVSLSRNQPGEIYVRSPQMMKGYLNDEEATKG+IDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDE+FIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQV 507
           VPVAFIVRSDGSNITEDEIKQYISKQV
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQV 507

BLAST of Csor.00g217590 vs. ExPASy TrEMBL
Match: A0A6J1E068 (4-coumarate--CoA ligase 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111429639 PE=4 SV=1)

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 499/506 (98.62%), Postives = 502/506 (99.21%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDHS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAIL QGYGMTESGSVLTMSLALAREKFAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPE GVSLSRNQPGEIYVRSPQMMKGYLNDEEATKG+IDE GWLH
Sbjct: 361 GACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDESGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGDMGLVDDDDE+FIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQ 506
           VPVAFIVRSDGSNITEDEIKQYISKQ
Sbjct: 481 VPVAFIVRSDGSNITEDEIKQYISKQ 506

BLAST of Csor.00g217590 vs. ExPASy TrEMBL
Match: A0A6J1JJ94 (4-coumarate--CoA ligase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484944 PE=4 SV=1)

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 488/507 (96.25%), Postives = 498/507 (98.22%), Query Frame = 0

Query: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60
           MAAAD SPEFIFRSKLPDLTIPNHLPLHTYCFERIS+FKHNPCLINAATGDIYTYGEVHV
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120
           TARRVAAG HKLGIGKGDVIMLLLQNTPQF FAFLGASFACAIATMANPFFTPAEIAKQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFIDSPPEGGCHFTSLTDEAKEEEMADVK 180
           ASSGAKLIITQAAFAEKVK LPQENQGILKVIFIDSPPEGGCHF+SLTDEAKEEE+ DVK
Sbjct: 121 ASSGAKLIITQAAFAEKVKNLPQENQGILKVIFIDSPPEGGCHFSSLTDEAKEEELTDVK 180

Query: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240
           FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH
Sbjct: 181 FSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFH 240

Query: 241 IYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQS 300
           IYSLSSIVM+SLRVGAAIL+VQKFDINSLVALVPKYRVTIATVVPPIVLA+VKSPVVD S
Sbjct: 241 IYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDCS 300

Query: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKS 360
           DMSSIRIVLSGAAPLGKNLEDAFRAK PHAILGQGYGMTESGSVLTMSLALA+E+FAIKS
Sbjct: 301 DMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERFAIKS 360

Query: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLH 420
           GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIID DGWLH
Sbjct: 361 GACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGDGWLH 420

Query: 421 TGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480
           TGD+GLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE
Sbjct: 421 TGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGE 480

Query: 481 VPVAFIVRSDGSNITEDEIKQYISKQV 507
            PVAFIVRSDGSNITEDEIK+YISKQV
Sbjct: 481 APVAFIVRSDGSNITEDEIKEYISKQV 507

BLAST of Csor.00g217590 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 694.9 bits (1792), Expect = 5.2e-200
Identity = 354/541 (65.43%), Postives = 433/541 (80.04%), Query Frame = 0

Query: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARR 64
           +++ + IFRSKLPD+ IPNHL LH Y F+ IS+F   PCLIN  TG +YTY +VHV +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 65  VAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSG 124
           +AA FHKLG+ + DV+MLLL N P+FV +FL ASF  A AT ANPFFTPAEIAKQA +S 
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 125 AKLIITQAAFAEKVKKLPQENQGILKVIFIDSP----PEGGCHFTSLTDEAKE--EEMAD 184
            KLIIT+A + +K+K L Q + G++ V   D+     PEG   FT LT    E  E +  
Sbjct: 138 TKLIITEARYVDKIKPL-QNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 197

Query: 185 VKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPL 244
           V+ SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+ +  S+DV+LCVLP+
Sbjct: 198 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 257

Query: 245 FHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVD 304
           FHIY+L+SI++  LRVGAAILI+ KF+IN L+ L+ + +VT+A +VPPIVLAI KS   +
Sbjct: 258 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 317

Query: 305 QSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAI 364
           + D+SSIR+V SGAAPLGK LEDA  AK P+A LGQGYGMTE+G VL MSL  A+E F +
Sbjct: 318 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 377

Query: 365 KSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGW 424
           KSGACGT++RNAEMKIV+P+TG SLSRNQPGEI +R  Q+MKGYLN+  AT   ID+DGW
Sbjct: 378 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 437

Query: 425 LHTGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVA 484
           LHTGD+GL+DDDDE+FIVDRLKELIKYKGFQVAPAELE LLI +  I DVAV+ MK+E A
Sbjct: 438 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 497

Query: 485 GEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540
           GEVPVAF+V+S  S ++ED++KQ++SKQVVFYKRIN+VFF +SIPK+PSGKILR+ LRA 
Sbjct: 498 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 557

BLAST of Csor.00g217590 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 685.6 bits (1768), Expect = 3.2e-197
Identity = 351/541 (64.88%), Postives = 429/541 (79.30%), Query Frame = 0

Query: 7   SPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVA 66
           S + IFRS+LPD+ IPNHLPLH Y FE IS+F   PCLIN  TG++YTY +VHVT+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 67  AGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAK 126
           AG H LG+ + DV+M+LL N+P+ V  FL ASF  AI T ANPFFTPAEI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 127 LIITQAAFAEKVKKLPQENQGILKVIF-IDSPPEGGCHFTSLT-DEAKEEEMADVKFSPD 186
           LI+TQ+ + +K+K L  +N G+L V    D+ PE    F+ LT  E    +    K SP+
Sbjct: 138 LIVTQSRYVDKIKNL--QNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 197

Query: 187 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSL 246
           DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+ +   +DV+LCVLP+FHIY+L
Sbjct: 198 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 257

Query: 247 SSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSS 306
           +SI++ SLRVGA ILI+ KF+I  L+  + + +VT+A VVPPIVLAI KSP  ++ D+SS
Sbjct: 258 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 317

Query: 307 IRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACG 366
           +R+V SGAAPLGK LEDA  AK P+A LGQGYGMTE+G VL MSL  A+E F +KSGACG
Sbjct: 318 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 377

Query: 367 TLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDM 426
           T++RNAEMKI++P+TG SL RN+PGEI +R  Q+MKGYLND  AT   ID+DGWLHTGD+
Sbjct: 378 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 437

Query: 427 GLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVA 486
           G +DDDDE+FIVDRLKELIKYKGFQVAPAELE LLI +  I DVAV+ MK+E AGEVPVA
Sbjct: 438 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 497

Query: 487 FIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLPANIP 546
           F+VRS  SNI+EDEIKQ++SKQVVFYKRIN+VFF DSIPK+PSGKILR+ LRA L   + 
Sbjct: 498 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 556

BLAST of Csor.00g217590 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 659.8 bits (1701), Expect = 1.9e-189
Identity = 326/531 (61.39%), Postives = 419/531 (78.91%), Query Frame = 0

Query: 11  IFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARRVAAGFH 70
           IFRSKLPD+ IPNHLPLHTYCFE++S     PCLI  +TG  YTYGE H+  RRVA+G +
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 71  KLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSGAKLIIT 130
           KLGI KGDVIM+LLQN+ +FVF+F+GAS   A++T ANPF+T  E+ KQ  SSGAKLIIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 131 QAAFAEKVKKLPQENQGILKVIFIDSPPEGGC--HFTSLTDEAKEEEMADVKFSPDDVVA 190
            + + +K+K L +     L +I  D P    C    T +TD+        V    DD  A
Sbjct: 154 HSQYVDKLKNLGEN----LTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLFHIYSLSSIV 250
           LP+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NP+ +++SNDV+LCVLPLFHIYSL+S++
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 MTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVDQSDMSSIRIV 310
           + SLR GA +L++ KF+I +L+ L+ ++RVTIA +VPP+V+A+ K+P V+  D+SS+R V
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 LSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAIKSGACGTLMR 370
           LSGAAPLGK L+D+ R +LP AILGQGYGMTE+G VL+MSL  A+E    KSG+CGT++R
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGWLHTGDMGLVD 430
           NAE+K+V+ ET +SL  NQPGEI +R  Q+MK YLND EAT   IDE+GWLHTGD+G VD
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 DDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAGEVPVAFIVR 490
           +DDE+FIVDRLKE+IK+KGFQV PAELE LLI++  I D AV+P  DEVAGEVPVAF+VR
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 SDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALL 540
           S+G++ITE+++K+Y++KQVVFYKR+++VFFV SIPKSPSGKILR+ L+A L
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Csor.00g217590 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 329/509 (64.64%), Postives = 404/509 (79.37%), Query Frame = 0

Query: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHVTARR 64
           +++ + IFRSKLPD+ IPNHL LH Y F+ IS+F   PCLIN  TG +YTY +VHV +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 65  VAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQAASSG 124
           +AA FHKLG+ + DV+MLLL N P+FV +FL ASF  A AT ANPFFTPAEIAKQA +S 
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 125 AKLIITQAAFAEKVKKLPQENQGILKVIFIDSP----PEGGCHFTSLTDEAKE--EEMAD 184
            KLIIT+A + +K+K L Q + G++ V   D+     PEG   FT LT    E  E +  
Sbjct: 138 TKLIITEARYVDKIKPL-QNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 197

Query: 185 VKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPL 244
           V+ SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+ +  S+DV+LCVLP+
Sbjct: 198 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 257

Query: 245 FHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIVKSPVVD 304
           FHIY+L+SI++  LRVGAAILI+ KF+IN L+ L+ + +VT+A +VPPIVLAI KS   +
Sbjct: 258 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 317

Query: 305 QSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAREKFAI 364
           + D+SSIR+V SGAAPLGK LEDA  AK P+A LGQGYGMTE+G VL MSL  A+E F +
Sbjct: 318 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 377

Query: 365 KSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDEDGW 424
           KSGACGT++RNAEMKIV+P+TG SLSRNQPGEI +R  Q+MKGYLN+  AT   ID+DGW
Sbjct: 378 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 437

Query: 425 LHTGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVA 484
           LHTGD+GL+DDDDE+FIVDRLKELIKYKGFQVAPAELE LLI +  I DVAV+ MK+E A
Sbjct: 438 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 497

Query: 485 GEVPVAFIVRSDGSNITEDEIKQYISKQV 508
           GEVPVAF+V+S  S ++ED++KQ++SKQV
Sbjct: 498 GEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Csor.00g217590 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 624.8 bits (1610), Expect = 6.6e-179
Identity = 322/547 (58.87%), Postives = 412/547 (75.32%), Query Frame = 0

Query: 5   DSSPEFIFRSKLPDLTIPNHLPLHTYCFERIS----KFKHNPCLINAATGDIYTYGEVHV 64
           + S +FIFRSKLPD+ IPNHLPL  Y F+R S          C+I+ ATG I TY +V  
Sbjct: 22  EPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQT 81

Query: 65  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 124
             RR+AAG H+LGI  GDV+MLLL N+P+F  +FL  ++  A++T ANPF+T  EIAKQA
Sbjct: 82  NMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQA 141

Query: 125 ASSGAKLIITQAAFAEKVKKLPQENQGILKVIFID-------SPPEGGC-HFTSLTDEAK 184
            +S AK+IIT+    +K+  L  +N G+L V   D       S  + GC  FT LT +A 
Sbjct: 142 KASAAKMIITKKCLVDKLTNL--KNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELT-QAD 201

Query: 185 EEEMADVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVV 244
           E E+   K SP+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENP+ +  +NDV+
Sbjct: 202 ETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVI 261

Query: 245 LCVLPLFHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAIV 304
           LC LP+FHIY+L +++++++R GAA+LIV +F++N ++ L+ +Y+VT+  V PP+VLA +
Sbjct: 262 LCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFI 321

Query: 305 KSPVVDQSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALA 364
           KSP  ++ D+SS+RI+LSGAA L K LEDA R K P+AI GQGYGMTESG+V   SLA A
Sbjct: 322 KSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFA 381

Query: 365 REKFAIKSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGI 424
           +  F  KSGACGT++RNAEMK+V+ ETG+SL RN+ GEI VR  Q+MKGYLND EAT   
Sbjct: 382 KNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATART 441

Query: 425 IDEDGWLHTGDMGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIP 484
           ID+DGWLHTGD+G VDDDDE+FIVDRLKELIK+KG+QVAPAELE LLIS+  I D AV+ 
Sbjct: 442 IDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVA 501

Query: 485 MKDEVAGEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILR 540
           MKDEVA EVPVAF+ RS GS +TED++K Y++KQVV YKRI  VFF++ IPK+ SGKILR
Sbjct: 502 MKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILR 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4ISH09.2e-21068.904-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1[more]
O241452.1e-20968.094-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
O241463.5e-20968.724-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
I3PB371.0e-20867.714-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P316841.3e-20868.464-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6589713.10.0100.004-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022921335.10.098.534-coumarate--CoA ligase 1-like isoform X1 [Cucurbita moschata][more]
XP_023515445.10.096.884-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022987367.10.096.334-coumarate--CoA ligase 1-like isoform X2 [Cucurbita maxima][more]
XP_022921336.10.098.624-coumarate--CoA ligase 1-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1E5C50.098.534-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JA640.096.334-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1E1380.098.624-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1E0680.098.624-coumarate--CoA ligase 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJ940.096.254-coumarate--CoA ligase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G51680.15.2e-20065.434-coumarate:CoA ligase 1 [more]
AT3G21240.13.2e-19764.884-coumarate:CoA ligase 2 [more]
AT1G65060.11.9e-18961.394-coumarate:CoA ligase 3 [more]
AT1G51680.31.1e-18464.644-coumarate:CoA ligase 1 [more]
AT3G21230.16.6e-17958.874-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 454..529
e-value: 1.6E-13
score: 51.4
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 33..445
e-value: 2.1E-105
score: 352.8
NoneNo IPR availableGENE3D3.30.300.30coord: 440..541
e-value: 3.3E-29
score: 103.5
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 7..538
NoneNo IPR availablePANTHERPTHR24096:SF3344-COUMARATE:COA LIGASE-LIKE PROTEINcoord: 7..538
NoneNo IPR availableCDDcd059044CLcoord: 21..535
e-value: 0.0
score: 753.686
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 15..538
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..439
e-value: 4.3E-156
score: 521.9
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 189..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g217590.m01Csor.00g217590.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016405 CoA-ligase activity