Csor.00g204240 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g204240 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 5.5e-297
Identity = 583/945 (61.69%), Postives = 684/945 (72.38%), Query Frame = 0

Query: 1   MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSP---GTTLTVSD 120
           S V SPNG        TT   KTS +KSS        +SS +A   KSP       TV D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 STDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVE 180
            +  Q         K++S+    +E  DSEDD+PLS  L GN  P     V +    K  
Sbjct: 121 RSQLQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK-- 180

Query: 181 KSSGD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL 240
           K++GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG 
Sbjct: 181 KNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGS 240

Query: 241 TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSK 300
           T  +++   G +  KRPL    + S+ Q S KK K+S S T +              G  
Sbjct: 241 TVKNEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRV 300

Query: 301 LVS--VKAKQ-------EVDDDDDHIPISQRMKKVAVSANKPLSSKKTVS---------- 360
           LVS  +KAKQ         DDDDD +PIS+R K  + ++N   +  K V           
Sbjct: 301 LVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKP 360

Query: 361 ---KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 420
               VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ H
Sbjct: 361 KARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPH 420

Query: 421 GVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 480
           G+KI+YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LD
Sbjct: 421 GIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLD 480

Query: 481 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 540
           DCDFTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPP
Sbjct: 481 DCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPP 540

Query: 541 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 600
           GLFRGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW 
Sbjct: 541 GLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWA 600

Query: 601 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 660
           DPINPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIA
Sbjct: 601 DPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIA 660

Query: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 720
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVE
Sbjct: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVE 720

Query: 721 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 780
           VE  V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML
Sbjct: 721 VEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEML 780

Query: 781 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 840
           ++ TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK 
Sbjct: 781 SQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKT 840

Query: 841 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 885
           +LDRAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTS
Sbjct: 841 NLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTS 900

BLAST of Csor.00g204240 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 8.8e-295
Identity = 569/936 (60.79%), Postives = 686/936 (73.29%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDEDDEDNMPIVFKRSS--ASRQNQLDSEAKQRLNKLSGRQGS 60
           MA E F K  +      ++++DED++P+VFKR+S  A+  N+         N   G   S
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKS 60

Query: 61  DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDS 120
               P      + NR+ S    S  +SS +     S+SS+Q   +KSP          D 
Sbjct: 61  S--PPMRSPLTSPNRSAS----SSTRSSMMKPALPSSSSVQRSTLKSP--------LRDD 120

Query: 121 QSKHLVMASVKEESDRHPT---NESSDSEDDKPLSFRLKGNS-------NPPGKGLVPTV 180
           +S   V+A  +    + P+   ++  DSEDDKPLS RLK +S       +  G+G     
Sbjct: 121 RS---VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQA 180

Query: 181 LKNKVEKSSGDS--------DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG 240
           ++    +  G S        D+  P+SS    K++   S       ++K+PL++ + +NG
Sbjct: 181 VQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNG 240

Query: 241 L---TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL------------- 300
           L   T G   Q P    +KRPL      SS Q S K+PKLS+ + P+             
Sbjct: 241 LKPKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQD 300

Query: 301 --GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKTVSKVVSSS 360
             G  L + K         K++  D DDH+PI+ RMK  + S NK  S+K + SK+++SS
Sbjct: 301 AKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKMIASS 360

Query: 361 MKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKIIYKGK 420
            +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVKI+++GK
Sbjct: 361 SRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGK 420

Query: 421 LVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYD 480
            VDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+
Sbjct: 421 PVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYE 480

Query: 481 WYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEH 540
           WY  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEH
Sbjct: 481 WYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEH 540

Query: 541 PKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFK 600
           PKMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFK
Sbjct: 541 PKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFK 600

Query: 601 YVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKL 660
           YVFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKL
Sbjct: 601 YVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKL 660

Query: 661 ALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAI 720
           ALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI
Sbjct: 661 ALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAI 720

Query: 721 TQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDV 780
            QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV
Sbjct: 721 GQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDV 780

Query: 781 AEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGK 840
            EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+
Sbjct: 781 PEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGR 840

Query: 841 PPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRI 885
            PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+DPRI
Sbjct: 841 TPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRI 900

BLAST of Csor.00g204240 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 4.3e-241
Identity = 487/825 (59.03%), Postives = 585/825 (70.91%), Query Frame = 0

Query: 99  SPGTTLTVSDSTDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFR-----LKGNSNP 158
           +PG T+   ++++   K     ++KEE      + S DS+DDKPLS +     L GNS+ 
Sbjct: 4   NPGITVIKQNASNVMEK-----TLKEEGQSR--SNSEDSDDDKPLSHKLSSGALNGNSHH 63

Query: 159 PGKG---LVPTVLKNKVEKSSGDSDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKV 218
              G     P+   +   +     +DE+ LSS+  + + +  S       DE KPL S  
Sbjct: 64  IRMGSNLSCPSPYTSPKPRIIKGPEDEMLLSSELQLNAGA--SNAESSDSDESKPLAS-- 123

Query: 219 HQNGLTSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQE 278
                    +L+ P    +KRPL      +S    SKK KLS+ S P   KL + + ++ 
Sbjct: 124 ---------ELEDPSSSLNKRPL--IQPKNSDPSPSKKAKLSEPSAPTNRKLKTAEQEEA 183

Query: 279 VDDDDDHIPISQRMKKVAVSANKPLSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTK 338
             DDD    IS R KK    A+K    KK     VS+S+ K        V  SK +K   
Sbjct: 184 AADDDP--SISNRNKKSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPL 243

Query: 339 LLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTD 398
           +L    +GQ     LVHNGVIFPP Y+ HGVK++Y+   VDLTPEQEEVATMFAVM +T+
Sbjct: 244 VLVKGRNGQ----PLVHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETE 303

Query: 399 YMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKE 458
           YM KPKF+ENF +DWRK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE
Sbjct: 304 YMTKPKFRENFMSDWRKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKE 363

Query: 459 EKMK-QEEKYMWAILDGV-KEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPK-DITINI 518
            K + ++EKYMWA++DG+ +EKVGNFRV    + +      K  ++K+ ++ + DITINI
Sbjct: 364 GKDETRKEKYMWAVVDGMSREKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINI 423

Query: 519 GKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKY 578
           GKDAPIPE PIPGERWKE+RHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+  +EKY
Sbjct: 424 GKDAPIPEPPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKY 483

Query: 579 EKARKLKDYIKNIR-NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGC 638
           EK+RKLKDYI+ IR  AYTKDF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVGC
Sbjct: 484 EKSRKLKDYIEGIRAAAYTKDFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGC 543

Query: 639 CTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLD 698
           CTLKVENV+   PN+L+F+FLGKDSI+Y+N VEVE  VF AI QF+SGK G DDLFD+LD
Sbjct: 544 CTLKVENVETKRPNILKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLD 603

Query: 699 TSKLNAHLKDLMPGLTAKVFRTYNASITLDDM-----LNRGTKDGDVAEKIVIYQHANKE 758
           TSKLNAHLK+LMPGLTAKVFRTYNASITLD+M     L+R TK GDVAEK+V+YQHANKE
Sbjct: 604 TSKLNAHLKELMPGLTAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKE 663

Query: 759 VAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKDADGKQKRN 818
           VAIICNHQRTVSKSH AQM RLNEKI EL+ +LKEL+ DL R  KGKPPLK++DGK KRN
Sbjct: 664 VAIICNHQRTVSKSHSAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRN 723

Query: 819 LTPE----------------------VLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 878
           L  +                       + KKI+QTN KIEK+ERD +TKEDLKTVALGTS
Sbjct: 724 LNLKRYKGKLLKLIQKLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTS 783

Query: 879 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KINYLDPRITVAWCKRH+VPIEKIFNKSLLAKFAWAMDV   FRF
Sbjct: 784 KINYLDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVVLIFRF 790

BLAST of Csor.00g204240 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 529.3 bits (1362), Expect = 8.6e-149
Identity = 341/834 (40.89%), Postives = 474/834 (56.83%), Query Frame = 0

Query: 131 TNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPLSSKFHMKSTS 190
           ++E SDS ++ PLS  L                 NK  KS  D DD IP+  +   +++S
Sbjct: 30  SDEESDSSENHPLSESL-----------------NKKSKSESDEDD-IPIRKR---RASS 89

Query: 191 RVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPK 250
           + +  N      KK   +KV  NG          G+ + K+     +     + +   PK
Sbjct: 90  KKNMSNS---SSKKR--AKVMGNG----------GLKNGKKTAVVKEEEDFNEIAKPSPK 149

Query: 251 LSDSSTPLGSKLVSVKA-KQEVDDDDDHIPISQRMKKVAVSANKPLSSKKTVSKVVSSSM 310
               S   GSK  +  A K+E  D DD +P+       AVS       K  +    S++ 
Sbjct: 150 HKRVSKANGSKNGAKSAVKKEESDTDDSVPLR------AVSTVSLTPYKSELPSGASTTQ 209

Query: 311 KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQ--THGVKIIYKG 370
            ++    +   +  K+  S  +     D  +KWT+L HNGVIF PPY+     VK+IY G
Sbjct: 210 NRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYEPLPKNVKLIYDG 269

Query: 371 KLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGK---NHVIQNLDDCDFT 430
             V+L PE EEVA  +A M +TD+ + P F++NF+ D+ K+  +   NH I+    CDFT
Sbjct: 270 NPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFNHNIKEFSKCDFT 329

Query: 431 PIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 490
            ++  ++ ++E+KK M  E+KKA+K++K ++EEKY W ILDG KEKVGNFR+EPPGLFRG
Sbjct: 330 QMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEKVGNFRIEPPGLFRG 389

Query: 491 RGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINP 550
           RG HPK G LKRR++P+ ITINIG+  P+PE P+PG +W EV+HDNTVTWLA W++ IN 
Sbjct: 390 RGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTVTWLATWHENIN- 449

Query: 551 KEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYL 610
              KYVFLAA SSLKGQSD +KYEK+RKLKDYI +IR  Y KD  S+    RQ   A YL
Sbjct: 450 NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQRGTAMYL 509

Query: 611 IDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPV 670
           ID  ALRAGNEK +DEADTVGCC+L+ E+V   PP  + F+FLGKDSI+Y N VEV+  V
Sbjct: 510 IDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSIRYYNEVEVDPQV 569

Query: 671 FKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 730
           FK +  F +  K   D +FD+L T+ LN +L  LM GL+AKVFRTYNAS T+ + L +  
Sbjct: 570 FKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNASYTMAEELKKMP 629

Query: 731 KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISEL---RDILKELKID 790
           K+  +A+KI+ Y  AN+ VAI+CNHQR+V+K+H  QM R  E+I  L   R  L+++ ++
Sbjct: 630 KNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQYQRMRLRKMMLN 689

Query: 791 LD-RAKKGKPPL------------------------------------------------ 850
           L+ +  K KP L                                                
Sbjct: 690 LEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENEKLAAEDPKSVLP 749

Query: 851 -----------------KDADGKQKR----NLTPEVLEKKISQTNAKIEKIERDMQTKED 885
                             DA+ K K+      + E LEK++++ N +I  +   M  K++
Sbjct: 750 ESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERINVMRTQMIDKDE 809

BLAST of Csor.00g204240 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.2e-145
Identity = 358/900 (39.78%), Postives = 497/900 (55.22%), Query Frame = 0

Query: 53  DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQK---ASNSSIQAPLVKSPGTTLTVSDS 112
           D  SP   +   +    S+ K +P       T K   A N     P  K P  + + SDS
Sbjct: 40  DEASPPQPNKKLKAENGSIKKLTPTPKPAPVTPKPTAAVNGKKPIP-KKKPVDSSSDSDS 99

Query: 113 TDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGK---GLVPTVLKNK 172
           +DS S          +SD    ++SSDS+ D   S     +S+ P K     +P      
Sbjct: 100 SDSSS----------DSD----SDSSDSDSDSSSSDSSSSDSDVPKKKPISKIPNKPSGT 159

Query: 173 VEKSSGDSDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGV 232
           V K+S +S    P ++     +T+ V         + KP   KV     +S D       
Sbjct: 160 VSKTSKNS----PKTTTTTTTTTTTV---------KSKP-APKVKPEQSSSDDSSSDSSD 219

Query: 233 ISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKK 292
            SS       DS S      +KPK   S  P   K   +  K+E        P+    K+
Sbjct: 220 DSSD------DSSSDSSSEEEKPKKKPSKKPAAVK-KEITVKKETTTTTKKTPVK---KE 279

Query: 293 VAVSANKPLSSKKTVSKVVS---SSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQK--- 352
           V V      ++ K V K  S      +KT  K K   +K K  +  +      +G     
Sbjct: 280 VEVKKETKTTTTKKVKKEESDDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWW 339

Query: 353 ----------KWTSLVHNGVIFPPPYQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTD 412
                     KWT+L+HNG +FPP Y  HG+K +Y GK+V LTP+QEEVAT +A   +TD
Sbjct: 340 DKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLETD 399

Query: 413 YMQKPKFKENFWNDWRKLL--GKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKAL 472
           +++K  FK+NF+ +++ LL   +  +++ +D CDF+ I+   +T+KE++K  + EEK A 
Sbjct: 400 HVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAE 459

Query: 473 KEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIG 532
             EK K +EK+ +A +DG KEKVGN+ +EPPGLF GRG+HPK G LK+RI P D+T+NIG
Sbjct: 460 TAEKKKIQEKHGFATIDGFKEKVGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIG 519

Query: 533 KDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYE 592
           +   IP  PIPGE+WKE+ H+NTVTWLAFW + +N   FKYV+LA SS +KGQ+D++K+E
Sbjct: 520 EGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFE 579

Query: 593 KARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCT 652
            AR LK+ I +IR  Y K F  +   +RQ+AVA YLID LALR GNEK +D+ADTVGCC+
Sbjct: 580 VARGLKNCIGDIRKGYQKSFNDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCS 639

Query: 653 LKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTS 712
           L+VE++K    N +  +FLGKDS++Y NTV++    +K +  F  GK  TD LFD L  +
Sbjct: 640 LRVEHIKLESNNTITLDFLGKDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVT 699

Query: 713 KLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR-GTKD-GDVAEKIVIYQHANKEVAIIC 772
           +LN HLK  M GL+AKVFRTYNASITL   L++  TK+   + EKI+ Y   N+EVAI+C
Sbjct: 700 QLNNHLKKQMTGLSAKVFRTYNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILC 759

Query: 773 NHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPP---------------- 832
           NHQ+   K+    MS+++EKI ELRD+      DL R K GK P                
Sbjct: 760 NHQKAPPKNLAETMSKIDEKIQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLP 819

Query: 833 ---------LKDADGK--------------QKRNLTPEV---LEKKISQTNAKIEKIERD 885
                     KD D +              +K    P+    +++KI++   +I+K E  
Sbjct: 820 KLIQLAITNKKDKDKEKTDKEISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEIL 879

BLAST of Csor.00g204240 vs. NCBI nr
Match: KAG6596930.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028410.1 DNA topoisomerase 1 alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1726 bits (4469), Expect = 0.0
Identity = 884/884 (100.00%), Postives = 884/884 (100.00%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL
Sbjct: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300

Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
           VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Csor.00g204240 vs. NCBI nr
Match: XP_022940477.1 (DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940487.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata] >XP_022940496.1 DNA topoisomerase 1 alpha-like [Cucurbita moschata])

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 875/884 (98.98%), Postives = 879/884 (99.43%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTL VSDSTDSQSKHLV A
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEESDRHPTNE+SDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG TSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVA+SANK LSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
           VK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Csor.00g204240 vs. NCBI nr
Match: XP_023520897.1 (DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo] >XP_023520898.1 DNA topoisomerase 1 alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1701 bits (4406), Expect = 0.0
Identity = 874/885 (98.76%), Postives = 878/885 (99.21%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MA+ETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAIETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSS QAP VKSPGTTL VSDSTDSQSKHLV A
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSTQAPPVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL
Sbjct: 121 AVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV-AVSANKPLSSKK 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKV AVSANK LSSKK
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVVAVSANKSLSSKK 300

Query: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360
           TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH
Sbjct: 301 TVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 360

Query: 361 GVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420
           GVK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD
Sbjct: 361 GVKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 420

Query: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480
           DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP
Sbjct: 421 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 480

Query: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540
           GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN
Sbjct: 481 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 540

Query: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600
           DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA
Sbjct: 541 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 600

Query: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE
Sbjct: 601 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 660

Query: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720
           VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML
Sbjct: 661 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 720

Query: 721 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780
           NRG+KDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI
Sbjct: 721 NRGSKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 780

Query: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840
           DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS
Sbjct: 781 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 840

Query: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 KINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885

BLAST of Csor.00g204240 vs. NCBI nr
Match: XP_023005283.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005284.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005285.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023005286.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])

HSP 1 Score: 1679 bits (4349), Expect = 0.0
Identity = 862/884 (97.51%), Postives = 871/884 (98.53%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTL VSDSTDSQS+HLV A
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVAVSANK LSSKKT
Sbjct: 241 -PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
           VK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of Csor.00g204240 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1538 bits (3983), Expect = 0.0
Identity = 794/889 (89.31%), Postives = 830/889 (93.36%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAPMFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKH 120
            NGQSSNAQ R TSLPKTSPM+  E+G+QKAS SSIQ PLVKS GTTL VSDS DS+ K 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRP-EIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVMASVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           L  A+VKEE+D  HPT ++SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGFDEKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPL 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK ++ + NK  
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMK-MSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKI++KGK VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Csor.00g204240 vs. ExPASy TrEMBL
Match: A0A6J1FIM6 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111446061 PE=3 SV=1)

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 875/884 (98.98%), Postives = 879/884 (99.43%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ
Sbjct: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTL VSDSTDSQSKHLV A
Sbjct: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLKVSDSTDSQSKHLVKA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEESDRHPTNE+SDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDD IPL
Sbjct: 121 AVKEESDRHPTNETSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDGIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG TSGDKLQKPGVISSKRPLPNADSHS
Sbjct: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGFTSGDKLQKPGVISSKRPLPNADSHS 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300
           SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVA+SANK LSSKKT
Sbjct: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVALSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
           VK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884

BLAST of Csor.00g204240 vs. ExPASy TrEMBL
Match: A0A6J1L1R3 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111498332 PE=3 SV=1)

HSP 1 Score: 1679 bits (4349), Expect = 0.0
Identity = 862/884 (97.51%), Postives = 871/884 (98.53%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSPNGQ 60
           MAVETFGKAP FDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCS NGQ
Sbjct: 1   MAVETFGKAPTFDEDDEDNMPIVFKRSSASRQNQLDSEAKQRLNKLSGRQGSDVCSANGQ 60

Query: 61  SSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKHLVMA 120
           +SNAQNRTTSLPKTSPMKSS+VGTQKASNSSIQA LVKSPGTTL VSDSTDSQS+HLV A
Sbjct: 61  TSNAQNRTTSLPKTSPMKSSQVGTQKASNSSIQASLVKSPGTTLKVSDSTDSQSRHLVKA 120

Query: 121 SVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSDDEIPL 180
           +VKEE+DRHPTNESSDSEDDKPLSFRLKG SNPPGKGLVPTVLKNKVEKSS DSDDEIPL
Sbjct: 121 AVKEENDRHPTNESSDSEDDKPLSFRLKGKSNPPGKGLVPTVLKNKVEKSSADSDDEIPL 180

Query: 181 SSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPNADSHS 240
           SSKFHMKS+SRVSGGNQHGFDEKKPLISKVHQNGL SGDKLQKPGVISSKRPLPNADSH 
Sbjct: 181 SSKFHMKSSSRVSGGNQHGFDEKKPLISKVHQNGLISGDKLQKPGVISSKRPLPNADSHL 240

Query: 241 SPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKT 300
            PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDD+DDHIPISQRMKKVAVSANK LSSKKT
Sbjct: 241 -PQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDNDDHIPISQRMKKVAVSANKSLSSKKT 300

Query: 301 VSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360
           VSKVVSSSMKKT KKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG
Sbjct: 301 VSKVVSSSMKKTFKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHG 360

Query: 361 VKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 420
           VK+IYKGKLVDLTPEQEEVATMFAVMKDTDYMQKP+FKENFWNDWRKLLGKNHVIQNLDD
Sbjct: 361 VKMIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPRFKENFWNDWRKLLGKNHVIQNLDD 420

Query: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480
           CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG
Sbjct: 421 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPG 480

Query: 481 LFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540
           LFRGRGEHPKMGKLKRRIHP DITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND
Sbjct: 481 LFRGRGEHPKMGKLKRRIHPSDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWND 540

Query: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600
           PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV
Sbjct: 541 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAV 600

Query: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV
Sbjct: 601 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 660

Query: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720
           ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN
Sbjct: 661 ELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 720

Query: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780
           RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID
Sbjct: 721 RGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKID 780

Query: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840
           LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK
Sbjct: 781 LDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSK 840

Query: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 INYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 883

BLAST of Csor.00g204240 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1537 bits (3980), Expect = 0.0
Identity = 795/889 (89.43%), Postives = 829/889 (93.25%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKA MFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKH 120
            NGQSSNAQ R TSLPKTSPM+  E+G+QKAS SSIQ PLVKS GTTL VSDS DS+ K 
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRP-EIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVMASVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           LV A+VKEE+D  HP  E+SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGFDEKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPL 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK ++ + NK  
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMK-MSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKI++KGK VDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Csor.00g204240 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1533 bits (3970), Expect = 0.0
Identity = 793/889 (89.20%), Postives = 828/889 (93.14%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAPMFD+DDEDNMPIVFKRSSASRQNQL+SE K    QRLN+LSGR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDSQSKH 120
            NGQSSNAQ R TSLPKTSPM+  E+G+QKA+ SSIQ PLVKS GTTL VSDS DS+ K 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRP-EIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVMASVKEESDR-HPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDSD 180
           L  A+VKEE+D  HPT E+SDSEDDKPLS RLKGNSN P KG +PTV KNK++KSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLPN 240
           DE+PLSSKF MKS S V GG QHGF EKKPL SKVHQNG TS DKLQKP V+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKPL 300
           ADSHSS Q SSKKPKLSDSSTPL +  VS+KAKQEV DDDDHIPISQRMK ++ + NK  
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMK-MSAAVNKSS 300

Query: 301 SSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPP 360
            SKKTVSKV SSS+KKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT+LVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKI++KGK VDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI P DITINIGKDAPIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTR 600
           AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILK 780
           DDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Csor.00g204240 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1511 bits (3913), Expect = 0.0
Identity = 784/890 (88.09%), Postives = 821/890 (92.25%), Query Frame = 0

Query: 1   MAVETFGKAPMFDEDDEDNMPIVFKRSSASRQNQLDSEAK----QRLNKLSGRQGSDVCS 60
           MAVETFGKAPMFD+DDEDNMPIVFKRSSASRQNQLDSE K    QRLNKL GRQGSD+CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  PNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSS-IQAPLVKSPGTTLTVSDSTDSQSK 120
            NGQSSN Q R TSLPKTSPM+S E+G+QKA  SS IQ PL KS G +L  SDS DS+ K
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRS-EIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 HLVMASVKEES-DRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVEKSSGDS 180
           +LV A+VKEE+   +P+ E+SDSEDDKPLS RLKGNSN P KGLV T+ KNKV+KSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGLTSGDKLQKPGVISSKRPLP 240
           DDE+PLSSKF MK  S   G  Q GFDEKKPL+ KVHQNG TS DKLQKP V+SSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDDDHIPISQRMKKVAVSANKP 300
           N DSHS  QFS+KKPKLSDSS+PL SKLVS+KAKQEVDDDDD IPISQR+KK   + NK 
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 LSSKKTVSKVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPP 360
            SSK TVSKVVSSS+KKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWT+LVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK++YKGK VDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEK+KQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI P DITINIGK APIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPT 600
           LAFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR LKDYIKNIRNAYTKDFTSKDPT
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDIL 780
           LDDMLNRGTKDG+V+EKIV YQHANKEVAIICNHQRTVSKSHGAQM+RLNEKISELRDIL
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGKQKRNLTPE LEKKISQTNAKIEKIERDM+TKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Csor.00g204240 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1021.5 bits (2640), Expect = 3.9e-298
Identity = 583/945 (61.69%), Postives = 684/945 (72.38%), Query Frame = 0

Query: 1   MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSP---GTTLTVSD 120
           S V SPNG        TT   KTS +KSS        +SS +A   KSP       TV D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 STDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVE 180
            +  Q         K++S+    +E  DSEDD+PLS  L GN  P     V +    K  
Sbjct: 121 RSQLQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK-- 180

Query: 181 KSSGD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL 240
           K++GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG 
Sbjct: 181 KNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGS 240

Query: 241 TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSK 300
           T  +++   G +  KRPL    + S+ Q S KK K+S S T +              G  
Sbjct: 241 TVKNEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRV 300

Query: 301 LVS--VKAKQ-------EVDDDDDHIPISQRMKKVAVSANKPLSSKKTVS---------- 360
           LVS  +KAKQ         DDDDD +PIS+R K  + ++N   +  K V           
Sbjct: 301 LVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKP 360

Query: 361 ---KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 420
               VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ H
Sbjct: 361 KARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPH 420

Query: 421 GVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 480
           G+KI+YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LD
Sbjct: 421 GIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLD 480

Query: 481 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 540
           DCDFTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPP
Sbjct: 481 DCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPP 540

Query: 541 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 600
           GLFRGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW 
Sbjct: 541 GLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWA 600

Query: 601 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 660
           DPINPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIA
Sbjct: 601 DPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIA 660

Query: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 720
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVE
Sbjct: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVE 720

Query: 721 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 780
           VE  V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML
Sbjct: 721 VEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEML 780

Query: 781 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 840
           ++ TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK 
Sbjct: 781 SQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKT 840

Query: 841 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 885
           +LDRAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTS
Sbjct: 841 NLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTS 900

BLAST of Csor.00g204240 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1016.5 bits (2627), Expect = 1.3e-296
Identity = 581/942 (61.68%), Postives = 681/942 (72.29%), Query Frame = 0

Query: 1   MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSP---GTTLTVSD 120
           S V SPNG        TT   KTS +KSS        +SS +A   KSP       TV D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 STDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVE 180
            +  Q         K++S+    +E  DSEDD+PLS  L GN  P     V +    K  
Sbjct: 121 RSQLQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK-- 180

Query: 181 KSSGD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL 240
           K++GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG 
Sbjct: 181 KNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGS 240

Query: 241 TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL--------------GSK 300
           T  +++   G +  KRPL    + S+ Q S KK K+S S T +              G  
Sbjct: 241 TVKNEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRV 300

Query: 301 LVS--VKAKQ-------EVDDDDDHIPISQRMKKVAVSANKPLSSKKTVS---------- 360
           LVS  +KAKQ         DDDDD +PIS+R K  + ++N   +  K V           
Sbjct: 301 LVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKP 360

Query: 361 ---KVVSSSMKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTH 420
               VVS   +   K +KK  K SKY  S+K  PSSGDGQKKWT+LVHNGVIFPPPY+ H
Sbjct: 361 KARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPH 420

Query: 421 GVKIIYKGKLVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLD 480
           G+KI+YKGK VDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LD
Sbjct: 421 GIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLD 480

Query: 481 DCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPP 540
           DCDFTPIY+W+  EKEKKKQM+TEEKKALKEEKMKQEEKYMWA++DGVKEK+GNFRVEPP
Sbjct: 481 DCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPP 540

Query: 541 GLFRGRGEHPKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWN 600
           GLFRGRGEHPKMGKLK+RIHP +IT+NIGK APIPECPI GERWKEV+HDNTVTWLAFW 
Sbjct: 541 GLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWA 600

Query: 601 DPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIA 660
           DPINPKEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIA
Sbjct: 601 DPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIA 660

Query: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVE 720
           VATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVE
Sbjct: 661 VATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVE 720

Query: 721 VELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDML 780
           VE  V+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML
Sbjct: 721 VEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEML 780

Query: 781 NRGTKDGDVAEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKI 840
           ++ TKDGDV +KIV+YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK 
Sbjct: 781 SQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKT 840

Query: 841 DLDRAKKGKPPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTS 882
           +LDRAKKGKPPL+ +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTS
Sbjct: 841 NLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTS 900

BLAST of Csor.00g204240 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1014.2 bits (2621), Expect = 6.3e-296
Identity = 569/936 (60.79%), Postives = 686/936 (73.29%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDEDDEDNMPIVFKRSS--ASRQNQLDSEAKQRLNKLSGRQGS 60
           MA E F K  +      ++++DED++P+VFKR+S  A+  N+         N   G   S
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKS 60

Query: 61  DVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSPGTTLTVSDSTDS 120
               P      + NR+ S    S  +SS +     S+SS+Q   +KSP          D 
Sbjct: 61  S--PPMRSPLTSPNRSAS----SSTRSSMMKPALPSSSSVQRSTLKSP--------LRDD 120

Query: 121 QSKHLVMASVKEESDRHPT---NESSDSEDDKPLSFRLKGNS-------NPPGKGLVPTV 180
           +S   V+A  +    + P+   ++  DSEDDKPLS RLK +S       +  G+G     
Sbjct: 121 RS---VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQA 180

Query: 181 LKNKVEKSSGDS--------DDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNG 240
           ++    +  G S        D+  P+SS    K++   S       ++K+PL++ + +NG
Sbjct: 181 VQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNG 240

Query: 241 L---TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPL------------- 300
           L   T G   Q P    +KRPL      SS Q S K+PKLS+ + P+             
Sbjct: 241 LKPKTEGHSSQAP----AKRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHISATQD 300

Query: 301 --GSKLVSVK--------AKQEVDDDDDHIPISQRMKKVAVSANKPLSSKKTVSKVVSSS 360
             G  L + K         K++  D DDH+PI+ RMK  + S NK  S+K + SK+++SS
Sbjct: 301 AKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDS-SNNKSSSAKPSSSKMIASS 360

Query: 361 MKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTSLVHNGVIFPPPYQTHGVKIIYKGK 420
            +   +K  K VK SKY KS+K LP SGDGQKKW +L HNGVIFPPPY+ HGVKI+++GK
Sbjct: 361 SRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGK 420

Query: 421 LVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYD 480
            VDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDDCDFTPIY+
Sbjct: 421 PVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYE 480

Query: 481 WYQTEKEKKKQMTTEEKKALKEEKMKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEH 540
           WY  EKE KKQMT EEK+ +KEEK+KQEEKYMWA+LDGV+E++GNFRVEPPGLFRGRGEH
Sbjct: 481 WYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEH 540

Query: 541 PKMGKLKRRIHPKDITINIGKDAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFK 600
           PKMGKLK+RI P DITINIGK+APIPECPIPGERWKEV+HDNTVTWLAFW+DPINPKEFK
Sbjct: 541 PKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFK 600

Query: 601 YVFLAASSSLKGQSDKEKYEKARKLKDYIKNIRNAYTKDFTSKDPTRRQIAVATYLIDKL 660
           YVFLAASSSLKGQSDKEKYEKARKL ++I +IR AYTKDF +KD T+RQIAVATYLIDKL
Sbjct: 601 YVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKL 660

Query: 661 ALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAI 720
           ALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  V+KAI
Sbjct: 661 ALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAI 720

Query: 721 TQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDV 780
            QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T+DGDV
Sbjct: 721 GQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDV 780

Query: 781 AEKIVIYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGK 840
            EK+V+YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDRAKKG+
Sbjct: 781 PEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGR 840

Query: 841 PPLKDADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRI 885
            PL  +DGK+KRNLTPE LEKKI QT  KIEK+ERDMQTKED+KTVALGTSKINY+DPRI
Sbjct: 841 TPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRI 900

BLAST of Csor.00g204240 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 427.2 bits (1097), Expect = 3.3e-119
Identity = 208/271 (76.75%), Postives = 244/271 (90.04%), Query Frame = 0

Query: 614 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 673
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 674 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVAEKIV 733
           GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 734 IYQHANKEVAIICNHQRTVSKSHGAQMSRLNEKISELRDILKELKIDLDRAKKGKPPLKD 793
           +YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+ 
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 794 ADGKQKRNLTPEVLEKKISQTNAKIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWC 853
           +DGK+ R+L P   EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 854 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 885
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 94.0 bits (232), Expect = 6.6e-19
Identity = 115/318 (36.16%), Postives = 157/318 (49.37%), Query Frame = 0

Query: 1   MAVETFGKAPMFDE---DDEDNMPIVFKRSS--ASRQNQLDSEAKQR----LNKLSGRQG 60
           M  ET  K P+ D    D +D+ P+ FKR++  AS  NQ  S +++       K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDVCSPNGQSSNAQNRTTSLPKTSPMKSSEVGTQKASNSSIQAPLVKSP---GTTLTVSD 120
           S V SPNG        TT   KTS +KSS        +SS +A   KSP       TV D
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSS------MPSSSSKASPAKSPLRNDMPSTVKD 120

Query: 121 STDSQSKHLVMASVKEESDRHPTNESSDSEDDKPLSFRLKGNSNPPGKGLVPTVLKNKVE 180
            +  Q         K++S+    +E  DSEDD+PLS  L GN  P     V +    K  
Sbjct: 121 RSQLQ---------KDQSECKIEHE--DSEDDRPLSSILSGNKGPTSSRQVSSPQPEK-- 180

Query: 181 KSSGD-------------SDDEIPLSSKFHMKSTSRVSGGNQHGFDEKKPLISKVHQNGL 240
           K++GD             SDDE P+SS F  K  S +SGGNQ   DEKKPL+ K+HQNG 
Sbjct: 181 KNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGS 240

Query: 241 TSGDKLQKPGVISSKRPLPNADSHSSPQFSSKKPKLSDSSTPLGSKLVSVKAKQEVDDDD 294
           T  +++   G +  KRPL    + S+ Q S KK K+S S T +  K  SV  K+E+ DD 
Sbjct: 241 TVKNEVPN-GKVLGKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSV--KKEI-DDK 283

BLAST of Csor.00g204240 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 131.0 bits (328), Expect = 4.9e-30
Identity = 61/68 (89.71%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 817 KIEKIERDMQTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 876
           KIEK+ERDMQTKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 877 DVDPDFRF 885
           DV+P F F
Sbjct: 62  DVEPHFTF 69