Csor.00g199960 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g199960
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	PI31_Prot_N domain-containing protein
Location	Csor_Chr03: 4844609 .. 4847619 (-)
RNA-Seq Expression	Csor.00g199960
Synteny	Csor.00g199960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTGGAGAAGTACAAAGAATCTACAAAGCAATACAAAAATGGTGAATGAACAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACGTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGGTATTACTTCTTTGTTTATTGAAAAATTTAAAATTCTTCCCATATTTAGTATTTCTGAGTCTTGGATTGGGGTTTAGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGAGTACGCTTTCCAAGATATCAGATATATATGTGTATTGGCAAATTAGTACGTTGTCCTGTTTCGGTATTGAGTGTTTTTTCGTTCCTATGGTTCTTTTAGCGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTCGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGTAGTGATTAGATCAGTAATCAACACGTACGGTTATTTTAATTCTTCATTTTTTCTGATGTTATTGAGACGATTACTCTTTCTTGTGATTCTTATTTCTTGGCGTGTTATAAATATGAATCGAAATTATGTCTCTGGTAATCGTTCTTTAATCTGGTTCTTGGCGGTGTACATGCTTTTTGCAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGGTCTTTCTCTCTATCCACACTTACGCAATCACAAAATGTCATGACATAATTTGAGAAATATGTTGGGTCTTGACTTAAGTTCTTAACTATTGTCTGCTGCCATGATTTTACGTTTGTGTTGGGTGCTATTATCACTGTCTCCAACATTCTGATTTGCTAGTTTTTAAGTTTCTAACAGTAATTTGAATGCATTTTCTTTATTATATCTTGTTTTCCCCTTTATGGTTAGATCAAGAAGATAATGGTTTATATGCTTTGTGCTTTGATTGATTGATTGATTTGATACCCAGTTCCTCTTAGTGGTTTATAGGCTTTGATTGATTTGACACAAGAAGATAATGGTTTCCTTTGAGAGAGATTGTAATAGTCTTACTTAATGTGCAGGAATTGTTTTTGCTGAATTATTGTGACGGCAAATATTTAATGGCAAGCATTTTGTGCCTTTGTCCAATCCTTAATTGTATCAACTTAGGAATTTTCTAAGGTTCCATGTTTACAAATAATTTAGCTATGAACTTGTATCCCATGAGTGAGTGAGTTGACATAGTCTGAATGTGGCTGTTTTTGCTATTCTCTTAGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCTGTTGCTGGAATGTACCCTACTGGGTAATTTATTTAAGTTCGTCGATCATTTGTGTACGTATGTGTAACAGTTCAAGCCTACCACTAGTAGATATTGTTTTCGTTGGGCATTCCCTCAAGGTTTTTAAAACGTGTTTGTTAGTTAGAGGTCTCCACAATCTTAAAAAGAATGTTTGTTCTCCTCTCCAATCGATGTGGGATCTCATAATTCAATCTCCTTCGAGGCCAGCATTCTCATTGGCACTTTATACTCTCTCCAATTGATGTGCGATCTCACATCGATTGGAGAGGGGAACGAAACATTTTTTATAAAGGTGTGGAAACTTATCCCTAGCAGACGTGTTTTAAAAACCTCGAGGGAAAACCCGAAAAGAAAAATACAACGAGAACAATATTTGCTAGTGATGGACTTGAGCTGAGCTGTTACAGTACGATTGTCGCAAAATATCATGTTAATTTTGTTTTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTATTGATTACAGAGGTGGCTTCAGCGGCGATGGAAGCATGCTTTTAGGTTGGTGAATTGTTAAGTCTCCTAGTATTAGATTGAAAATAAGCGTAATTTCACTATTTTATCATGCAGGACCTAATGACCCGCGATGGTTAGGAACTGGTAAAGGACCTGGATTTGAAGGCGGGCAGCCGTGAGTCAATTAGCTCTCACTCTCACTCATATAGACACACACAAACACTCACCTTTGCATTATGACTGCTGCTAATTCCAAGTGTCTTGAAACAGGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGCTAGGTATATGGAATTTTCCTTACCGTGCCATTGCATATTGGGGTGGGGGGCTCTCTCTTTGAATCATTAATTTCTATCAGCGTTTATCATTTGCTGAATGCCTGCTTTGACAACGACTAAATTCAAACCATGTATCTTGGCATTCTAAATTAGGATATGTTCATACAAACAACTTGTTTCTGAATACATTTTGTATGGAGCGGCAGAGTAATTAATTAACTGGGATGCTTCTTCTTCTTCTTCTCTTCTTCTTCTCTTCTTCTTCCCCTTCTTCTTCTTCCCTTTGTTACAGAAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAACATTTTGGAGACGGTTCAGATTTCATATAGTCCGCCATAGTTATAAAGCTCTGAAAGTTGTTGAAAGTGTTGGGGAAAGACGATAAATTTGAGGGATGGATGGTCTACCCATTTTATGCATATGCTTGTATTGAGTTCCTTTTTTCTTCTGTTTTAGGTGGCCAGTAATGACTGTTATGTACGGTGAATATAGGAATGGTGTTTACTGA

mRNA sequence

ATGAATTGGAGAAGTACAAAGAATCTACAAAGCAATACAAAAATGGTGAATGAACAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACGTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGACGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTCGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCTGTTGCTGGAATGTACCCTACTGGAGGTGGCTTCAGCGGCGATGGAAGCATGCTTTTAGGACCTAATGACCCGCGATGGTTAGGAACTGGTAAAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGCTAGGTGGCCAGTAATGACTGTTATGTACGGTGAATATAGGAATGGTGTTTACTGA

Coding sequence (CDS)

ATGAATTGGAGAAGTACAAAGAATCTACAAAGCAATACAAAAATGGTGAATGAACAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACGTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGACGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTCGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCTGTTGCTGGAATGTACCCTACTGGAGGTGGCTTCAGCGGCGATGGAAGCATGCTTTTAGGACCTAATGACCCGCGATGGTTAGGAACTGGTAAAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGCTAGGTGGCCAGTAATGACTGTTATGTACGGTGAATATAGGAATGGTGTTTACTGA

Protein sequence

MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSGDGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWPVMTVMYGEYRNGVY

Homology

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BLAST of Csor.00g199960 vs. ExPASy Swiss-Prot
Match: Q9M330 (Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 8.4e-75
Identity = 164/287 (57.14%), Postives = 199/287 (69.34%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           D    +   +YS Q++NLD LV  L S I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 195 KPIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPVAGMYPTGGGFSGDGSMLLGPNDPRW 254
            P P   GP + PSGVV+PPI   GG  DLFPGP AGMYP  GGF GDGSML+GP DPR+
Sbjct: 181 TPNP--LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTDPRF 240

Query: 255 LGTGKG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFARWP 296
              G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P
Sbjct: 241 FPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQP 281

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BLAST of Csor.00g199960 vs. ExPASy Swiss-Prot
Match: Q92530 (Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-06
Identity = 63/225 (28.00%), Postives = 100/225 (44.44%), Query Frame = 0


Query: 72  KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 131
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 132 DVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGS-SKATTSNPAR----AESESS 191
           +++D ++     ++   Y+N + L  R+ SGI++ +     KA  S+P R    A +   
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 192 ERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFP-GPVAGMYPTGGGFSGDGSML 251
           +   +  + P      P  DP G  +     +GG DL P GP  G        SG    L
Sbjct: 170 DPLRIPPHHPHTSRQPPWCDPLGPFV-----VGGEDLDPFGPRRGGMIVDPLRSGFPRAL 229

Query: 252 LGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGV--PGFEP 289
           + P+    L     PG       VPPGARFDP+GP G   PG  P
Sbjct: 230 IDPSS--GLPNRLPPG------AVPPGARFDPFGPIGTSPPGPNP 258

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BLAST of Csor.00g199960 vs. ExPASy Swiss-Prot
Match: Q5RDN3 (Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 5.4e-05
Identity = 62/225 (27.56%), Postives = 98/225 (43.56%), Query Frame = 0


Query: 72  KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 131
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 132 DVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGS-SKATTSNPAR----AESESS 191
           +++D ++     ++   Y+N + L  R+ SGI++ +     KA  S+P R    A +   
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 192 ERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFP-GPVAGMYPTGGGFSGDGSML 251
           +   +  + P      P  DP G        +GG DL P G   G        SG    L
Sbjct: 170 DPLRIPPHHPHTSRQPPWCDPLGPF-----AVGGEDLDPFGHRRGGMIVDPLRSGFPRAL 229

Query: 252 LGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGV--PGFEP 289
           + P+    L     PG       VPPGARFDP+GP G   PG  P
Sbjct: 230 IDPSS--GLPNRLPPG------AVPPGARFDPFGPIGTSPPGPNP 258

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BLAST of Csor.00g199960 vs. NCBI nr
Match: KAG6603621.1 (putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620 bits (1598), Expect = 7.32e-224
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSG 240
           AESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSG
Sbjct: 181 AESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSG 240

Query: 241 DGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWPVMTVM 300
           DGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWPVMTVM
Sbjct: 241 DGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWPVMTVM 300

Query: 301 YGEYRNGVY 309
           YGEYRNGVY
Sbjct: 301 YGEYRNGVY 309

[Close]

BLAST of Csor.00g199960 vs. NCBI nr
Match: XP_022950229.1 (probable proteasome inhibitor isoform X1 [Cucurbita moschata])

HSP 1 Score: 571 bits (1472), Expect = 1.70e-204
Identity = 289/298 (96.98%), Postives = 291/298 (97.65%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGG 240
           AESESSERSSVVVNKP   IPELPGPLLDPSGVVIPPINPLGGGDLFPGP AGMYPTGGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGG 240

Query: 241 FSGDGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           F GDGSMLLGPNDPRWLGTG+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFAR P
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNP 298

[Close]

BLAST of Csor.00g199960 vs. NCBI nr
Match: XP_023545137.1 (probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534 bits (1375), Expect = 6.13e-190
Identity = 271/284 (95.42%), Postives = 272/284 (95.77%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180

Query: 195 KP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSGDGSMLLGPNDP 254
           KP   IPELPGPLLDPSGVVIPPINP GGGDLFPGP AGMYP GGG SGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGAGMYPAGGGLSGDGSMLLGPNDP 240

Query: 255 RWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           RWLG G+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF R P
Sbjct: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNP 284

[Close]

BLAST of Csor.00g199960 vs. NCBI nr
Match: XP_022978238.1 (probable proteasome inhibitor isoform X1 [Cucurbita maxima])

HSP 1 Score: 531 bits (1367), Expect = 1.02e-188
Identity = 271/284 (95.42%), Postives = 273/284 (96.13%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           +IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 195 KP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSGDGSMLLGPNDP 254
           KP   IPELPGPLLDPSGVVIPPINPLGGGDLFPGP A MYP GGGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 255 RWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           RWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF R P
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNP 284

[Close]

BLAST of Csor.00g199960 vs. NCBI nr
Match: XP_022950231.1 (probable proteasome inhibitor isoform X3 [Cucurbita moschata])

HSP 1 Score: 509 bits (1311), Expect = 1.78e-180
Identity = 260/295 (88.14%), Postives = 262/295 (88.81%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSG 240
                                        VVIPPINPLGGGDLFPGP AGMYPTGGGF G
Sbjct: 181 -----------------------------VVIPPINPLGGGDLFPGPGAGMYPTGGGFGG 240

Query: 241 DGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           DGSMLLGPNDPRWLGTG+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFAR P
Sbjct: 241 DGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNP 266

[Close]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL
Match: A0A6J1GF71 (probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 8.25e-205
Identity = 289/298 (96.98%), Postives = 291/298 (97.65%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGG 240
           AESESSERSSVVVNKP   IPELPGPLLDPSGVVIPPINPLGGGDLFPGP AGMYPTGGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGG 240

Query: 241 FSGDGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           F GDGSMLLGPNDPRWLGTG+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFAR P
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNP 298

[Close]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL
Match: A0A6J1ISG6 (probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 531 bits (1367), Expect = 4.91e-189
Identity = 271/284 (95.42%), Postives = 273/284 (96.13%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           +IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 195 KP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSGDGSMLLGPNDP 254
           KP   IPELPGPLLDPSGVVIPPINPLGGGDLFPGP A MYP GGGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 255 RWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           RWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF R P
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNP 284

[Close]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL
Match: A0A6J1GE95 (probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 8.64e-181
Identity = 260/295 (88.14%), Postives = 262/295 (88.81%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKPIPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSG 240
                                        VVIPPINPLGGGDLFPGP AGMYPTGGGF G
Sbjct: 181 -----------------------------VVIPPINPLGGGDLFPGPGAGMYPTGGGFGG 240

Query: 241 DGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           DGSMLLGPNDPRWLGTG+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFAR P
Sbjct: 241 DGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNP 266

[Close]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL
Match: A0A6J1GF52 (probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 6.22e-180
Identity = 264/298 (88.59%), Postives = 266/298 (89.26%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180
           DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 181 AESESSERSSVVVNKP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGG 240
           AESESSERSSVVVNKP   IPELPGPLLDPSG                          GG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPSG--------------------------GG 240

Query: 241 FSGDGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           F GDGSMLLGPNDPRWLGTG+GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFAR P
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNP 272

[Close]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL
Match: A0A6J1ITK3 (probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 472 bits (1214), Expect = 3.84e-166
Identity = 248/284 (87.32%), Postives = 250/284 (88.03%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           +IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 195 KP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGGFSGDGSMLLGPNDP 254
           KP   IPELPGPLLDPSG                          GGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSG--------------------------GGFSGDGSMLLGPNDP 240

Query: 255 RWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295
           RWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF R P
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNP 258

[Close]

BLAST of Csor.00g199960 vs. TAIR 10
Match: AT3G53970.1 (proteasome inhibitor-related )

HSP 1 Score: 282.0 bits (720), Expect = 6.0e-76
Identity = 164/287 (57.14%), Postives = 199/287 (69.34%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           D    +   +YS Q++NLD LV  L S I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 195 KPIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPVAGMYPTGGGFSGDGSMLLGPNDPRW 254
            P P   GP + PSGVV+PPI   GG  DLFPGP AGMYP  GGF GDGSML+GP DPR+
Sbjct: 181 TPNP--LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTDPRF 240

Query: 255 LGTGKG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFARWP 296
              G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P
Sbjct: 241 FPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQP 281

[Close]

BLAST of Csor.00g199960 vs. TAIR 10
Match: AT3G53970.2 (proteasome inhibitor-related )

HSP 1 Score: 239.2 bits (609), Expect = 4.4e-63
Identity = 139/254 (54.72%), Postives = 173/254 (68.11%), Query Frame = 0


Query: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 75  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194
           D    +   +YS Q++NLD LV  L S I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 195 KPIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPVAGMYPTGGGFSGDGSMLLGPNDPRW 254
            P P   GP + PSGVV+PPI   GG  DLFPGP AGMYP  GGF GDGSML+GP DPR+
Sbjct: 181 TPNP--LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTDPRF 240

Query: 255 LGTGKG---PGFEG 265
              G G   PGF G
Sbjct: 241 FPFGDGSDRPGFMG 248

[Close]

BLAST of Csor.00g199960 vs. TAIR 10
Match: AT1G48530.1 (CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST Arabidopsis thaliana protein match is: proteasome inhibitor-related (TAIR:AT3G53970.2); Has 43 Blast hits to 43 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 11; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 133.7 bits (335), Expect = 2.6e-31
Identity = 80/179 (44.69%), Postives = 108/179 (60.34%), Query Frame = 0


Query: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60
           MN   T   +S   M    +VM VIR+  P FRN NDK+AF VHA    SG +LT+TG P
Sbjct: 1   MNLTRTPQTES---MATTHAVMGVIRSVMPAFRNKNDKIAFVVHASLAVSGFILTSTGRP 60

Query: 61  AFT-DAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDAL 120
           AF  DA  SS ++  + IEGWNE ++EYA+VY         K+ +VKCL MN KLL+DA+
Sbjct: 61  AFAHDALSSSTTQCLVGIEGWNEFDEEYAFVY-----KCPVKRYLVKCLAMNDKLLVDAI 120

Query: 121 TDENSEPLHLEIDVEDIVNPNG-GSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSN 178
            ++  E  HL+I+V + ++ +G   +Y TQ++N D LV  L S IL KL   S  T S+
Sbjct: 121 AEDGKEFGHLQIEVGNYIDESGEEGDYDTQFKNFDKLVTELKSEILCKLNTVSTTTKSS 171

The following BLAST results are available for this feature:

BLAST of Csor.00g199960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M330	8.4e-75	57.14	Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 ...	[more]
Q92530	1.3e-06	28.00	Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2	[more]
Q5RDN3	5.4e-05	27.56	Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1	[more]

BLAST of Csor.00g199960 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603621.1	7.32e-224	100.00	putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022950229.1	1.70e-204	96.98	probable proteasome inhibitor isoform X1 [Cucurbita moschata]	[more]
XP_023545137.1	6.13e-190	95.42	probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022978238.1	1.02e-188	95.42	probable proteasome inhibitor isoform X1 [Cucurbita maxima]	[more]
XP_022950231.1	1.78e-180	88.14	probable proteasome inhibitor isoform X3 [Cucurbita moschata]	[more]

BLAST of Csor.00g199960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GF71	8.25e-205	96.98	probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ISG6	4.91e-189	95.42	probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GE95	8.64e-181	88.14	probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1GF52	6.22e-180	88.59	probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ITK3	3.84e-166	87.32	probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]

BLAST of Csor.00g199960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53970.1	6.0e-76	57.14	proteasome inhibitor-related	[more]
AT3G53970.2	4.4e-63	54.72	proteasome inhibitor-related	[more]
AT1G48530.1	2.6e-31	44.69	CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021625	PI31 proteasome regulator, N-terminal	PFAM	PF11566	PI31_Prot_N	coord: 29..168 e-value: 1.8E-15 score: 57.0
None	No IPR available	GENE3D	3.40.1000.30		coord: 15..182 e-value: 8.5E-41 score: 141.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 167..192
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 167..195
IPR045128	Proteasome inhibitor PI31-like	PANTHER	PTHR13266	PROTEASOME INHIBITOR	coord: 15..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g199960.m01	Csor.00g199960.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0070628	proteasome binding