Csor.00g194060 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g194060
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCsor_Chr01: 2252742 .. 2258227 (+)
RNA-Seq ExpressionCsor.00g194060
SyntenyCsor.00g194060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGCGAAATGTGAAGGTCTTGGCATTGGAGATGTTTTCTCTGATGGAAATTGTGGCGGTTTTCCTTCGTTTCTTCCTAAAGAGGTCGCTAAAATCAAAGATCCATTTGCTAGAACCATGGCTCGCAAAATCGAGAGGCTACCGGTAATACGCTTTCTCTTTCACTATTCTTTCTCTTCTTGTGTTTTATTCGATGAAATCAATGGTGCATTGATCTTACACCGAGGAGTTTGCTTTTGTTTCTCCTAGTTTCTGCTTCTTATGTGTCTTATTCGGTTTCTTCTTCGTTTTGAATTGTTTTGGTTTTGTCTGCCTTTTTTTTATCGAGTGGTTTGAAGATGAAATTGAAGTATGGTAAAGCACTGTGACGATCATTGTTCTTGTTAGAGACGTCTCTGTCGCCGGCTTATTTTTTCCTCTCTTGATTGATCTTTGTGATTTTCGAACAACGTCTGTTTCCTGTAGTATTAAACCAAAAACAACCACGGAATTTCTCGTCGTGATAATCGCCTTTCTCGTGTATACTGCGTGCTTACTTTCTTGTCAGCGTCTGAGAAGGAATCGAGCTATAAGATTCGTTTACAATGTGATTTTTACTGCTTAGAAACTGGACTCGAATTTACTTCGCCATTGTATGTTGGGAATCCATTACGAGAGATTTGCAGATGGGTGGATTTCTTTCCCGACCCACTGTGTCACTTCTCGTGTTTATTGAAGATTATTTGCACTACGATCCTTTATATTACTTGTCCCTTTTTGCAGATCACATTATCAGAAACTCGCCTTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGCAAGAAGAGTCCAGTGGTTCTACTCCATTGCTTTGACAGGTCTGTTATGGAATCATCAAGATTTGTTCTATGAATTTTTTAAATATGTACTGTTTCCATTTAACGTGGTATGTGTTCATTGCTTCTTATCACACAGTTCTTGTTTGGAATGGAGTTATGCGTTTCCTCTTCTTGAAGAGGCTGGACTAGAAACGTGGGCTGTTGATGTTCTTGGATGGGGCTTTTCTGACTTGGGTGAGTTCATTTGTTTATATTTTCTGCTTCCTAATTGTATTTTATAAACTCTAAAAACGTTTCTCACGCTTATATGTTTCCTGATTATACTAATGTATACTAGATAGTCTTCCTCCATGTAATGTGCAAACGAAGCGCTCTCATCTGTACCAGGTTAATCACTTTACCTTCTTCACATTGCTTTTTTTATAAGGATTATTGGGGTCTCGATTTATTCATGTGTTCTTACGTCTGAAAAGTGCTCGAAACTTGGAGAATGATCATTCTTTGTGAAGTTTTATTTGTTGATGTAATGGCTTGGATTCACAACAGTAGTTTTGGACTATAATTAAATTTTAATTTGGGTTTTAGTTTTGGAAGTCCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGTGCAGCTGTTGCACTTGACTTTGCAGTTAATCATCCGGAAGCTGTAAGTATTTATTTTCTATCTGGCCTAGAGTAATGCAAGTATACTGAGAATTTGTGCATGAAGAATGTGAACAATGTAGTTCGAGCTCTCATATCACATGATTACTTTATCCTTAGATGTGTAAGAAATGAAGGAAACTGAGTTATTATCGCCAATGAACTGTTGGATATCTCTTATTATCATTGCTCCATATTGCAGCTCTTTTATTTTGATTATGTATGAGAGGAAGGATACCGTGTTCTGATATGTTTTTTTTCTTCTGAGAACTCGATACCATATACAAGTTCCAACAAATAGTGGTTCGACTTATAATGAAGCCTATGTCCGTTAGGCTTTTCCTCAAGAAGTTCACTATAAGTCTGTAACACTCTTGCCAGTGAGAATCAAAACGTACAAAACCTTTTTTCTTCTGCTTTCTTTTCAGGCTGAAAAGAAATTCCAGTTCTTTTCTGTATTGGTTCTCAATCCCACCAATTTCCTATCAAGACACACCTCATTACAAGTTCAAACCAACAAGAAGAAGGAAATTTCAAAACCTCCAGACACAGACTCACAAGTATTTCACAAAAAACCAATTTCAAAATACGCGCTATAAAGTTCTCTCACAAAAATAATGAGGAAATTGAAGTTGAGGAATAGGGTATGGGGAATTTTATTTAGAGAAGAGACCGAGTAAAAAAGGATTTGGAGGAGAAGGTTGGTTGGTAAGGTTGGGTTAACTCTGACCACTGGATCGAGATTAACAGATCCAAGGGAGGAGATAAATTGTGGGAAATTAAGGGGAAAAACGGAAAACCACTCTAGTAAGGGATTTGGATCTGGAGTGTCTAAAAGAGAAACAACCTTGCGTGGACGTTGCGCATGTGAGCCAAAAGCTTGTCTAGAAGTGGAAAAACTCTTGCATGGAGGTTTCAAATGTGTCGCAAGCATGTCTGGAAGTGGAAAAACTCTTACGTGGGGTTTCACAAGTACATAAGCATGTCTGGAAATGGAAAAAACTCTTACGCGGAGGTTTCACATTTGAGTCACGAGCATGTCTGGAAGTAACAAAGGGGGTGCATGTATCAGATGTGATGTGTGAGCATAGGGCCTAAAACTAGCGCTTTTTTTTAATACAGCTCTTCCATGTGTCGCCTGCTGGTCATAGCGATTGAGTTTGCTGCCATACATATTGCCTCATCGGCCTACCATGCCATATTGCAGTGTCATTGGTCAATTTAATTTCTAGTTTACAACAGGTGGATGTTTAGAATTGAAAATTTACACATAGCACCATCCTTCAGGGTGCTTTCTAATTGATCTTGATAATATATACTTACCAAATTATCTCATTAAAAGTTTATGTGAAACAAAGGCATCAAACTTACAGAAACAAAGGTCCTTGCAGCCTGGAAATTCTAGACCCGGAATCATCTTCCAGGCTCTTAATTTTTAGTGGTAAAATTTTTTTAATGACTGTTTCACAGGTGGAAAAGCTGGTTTTGATCAATGCTTGTGTGTATAGCAACTGCACTGGGCATCCTACAAAGCTGCCGAAAGTGGTAGCATATGCTGGGGTAGGTCTTCATGGCACATAACGTAGTGCATGCCAGTAATTAATTTGCCATTTCTTTTCTTGTAAACTTCATTAAGTTTAGATTACAATTTAACAATTTTAAACTACTTTTGGAAGATATGATAAGTTGGCTGAGTCAACAACTTTTTTAAGCTCTTATTGTCTTCTTCTCACAAAATTGTTTACTCCGTACTGCGTTATCTTGTTAAATTTTTGTTTTACTAAACACTTCTCATTTGCAATAATACTTTCAAGCCTGTGAATTCAATACTAGAAAAAGAACCGCCATGACATAAACTCTTTCCATAACCTTTTATTTTTTGTTTTTGCCAACTAGGATATCTATGTGGTTAAGGCATCCGAATTTTCTCTAGGAGATTTGTAGATTTAAATATCCGCCTAACATGTGATTTAATATTCTTGAGGAGTTATTAACGAAGTAAACTACGTATGCAGATTTGACTTTCTTTACTCTTCAAATAAGATTTAAGTGCTTGATTTTAGGAAATGTCTGTCATGATGACAAGCCCCGAAGCTTGTATAGCGCTGACAGGTACTTTTTAACGAATTGTATATCAGGTTTCTTTGTTGAAAAGCATCCCATTGCGCCTCTTAGCAAATAAGTTAACCTTCAATGAACTTTCACTTGCTCGAAATGTGGAATGGACGAATGTAAGTTTCTATTGTATCCTTCCTGTATAAGACTGCTGTTGAATATGCCATTAAATAAGGCATTAACCTACAATCTCAGACACTTAGTTGACTGATGGATGGTCTCTTTCTTTTCCCCTGCCGTCGTTTTTTAATCTTTTCGTTTATAACTCGAAGTATTTTCAATTCCATCAAATCCTTCTTGACGGTAACAAAATTTATTTGTCATAAATATGAAAGTGAGATATTTCTTGTTTAGCTGCAGATTTCCTATCTTGTTTAGTTGGTGTGTGATGACATGGCAATCTACTTACAATATTGTTATTGTACGGATTAGGTTGGCCGTTTACATTGTCTATTGCCTTGGTGGGAAGATGCAACTGTTAATTTTATGTACAGCGGAGGCTACAATGTAGTTTCTCTGATTAAACAGGTATATCAATCAATAGCCATTACGATGCTGGAATTTTAACTCATTTTTGGATTTCTTTTTATTAAATCTGAGTTTCATATGCTGAAATATTCTGATAAATTAAATTCAACTTTTCCATCAGTATTTCCAATGATTTACATTAACCTTGGTTTTTGGATAATGAAGGGCCAAATCAGAAATAGTAGGACCATGTTGTGGGGTAATTTTTTCATGGTGTTTAGCTGCCTACTTGATTAGTATTATTCTTTTTCCTACTCCTAAACATAATCTAGAAATCTTAACTGAACCTGCGACCACAGTGGTTTGGGATTCTTGAGTTGAAATGAAACATCATGGTTAAAAAGTGAATAATTTTTCAAATGTTATATATTGTATAGGCTCAGATGATAGATAGTTCGTGTCCCTGCTGTATGATAACGTTATATTTACACTATTCTCTGTACTTAATCAATAGGTCAAGCACAGAGCACTCATCATTGCAGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGGTGAGTTTTAATTTCTACTAATGAACTTAAAATCTATAAATAAAGATAGGAAACAAAGTCCATCGGAAAGAAACTATGATATCCCACCTCGGTTGAAGAGGGGAAGGAAGCATTTTTTAAAAGGGTGTGTAAACCTCTTCCTAGCATCAAACGCATTTTAAAAACCTTGAGGGTAAATTCGAAAAGGAAACCTCAAAGATGACAATATCTGCTAGCTGTGGGCTTGAGTTGTTACAGAAATCATCTATTCTGTTAAATTGCTACTGCCTTGAGGGTAAATTCGAAAAGGAAATCCCGAAGATGACAATATCTGCTAGTGGTGGGCTTGAGTTGTTACACTATTCTGTTAAATTGCTCAGCTGTTCATTTCATGGTTCTTGAACTTTCAAACCTTCTTGTTTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTCCTGAACTTCACACAAAAATCCAATTGAGTCCTTGTGAATGTTCTATAAGTTATTTATCAGAATCTTAACACAGAAAAAGACATTTATTTCCTTTTAAAATTTTCTTCTATGAATAGATGACTACATAAATGAGTATTTGAAGCCACGTTGGTAGGGTTTCGTTTTCCTTTTTCTTGAACTGTTTGCTTGTCAAAATAACAACTCGTGGACTGTTTTTGCCGACAAAATGTGCTTAAACAGTGATATTGTTGTTTGGTTTTGCAGAGGCTGCACTGTGAACTACCAAATGCAATAATGCGAATAATAACAGACAGTGGCCATCTGCCCCATGTTGACAGTCCTGCTTCTGTTTCAAAGCTTATTACAGAGTTTGTATTTCATGAATCCTGCTAG

mRNA sequence

ATGTCCGCGAAATGTGAAGGTCTTGGCATTGGAGATGTTTTCTCTGATGGAAATTGTGGCGGTTTTCCTTCGTTTCTTCCTAAAGAGATCACATTATCAGAAACTCGCCTTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGCAAGAAGAGTCCAGTGGTTCTACTCCATTGCTTTGACAGTTCTTGTTTGGAATGGAGTTATGCGTTTCCTCTTCTTGAAGAGGCTGGACTAGAAACGTGGGCTGTTGATGTTCTTGGATGGGGCTTTTCTGACTTGGATAGTCTTCCTCCATGTAATGTGCAAACGAAGCGCTCTCATCTGTACCAGTTTTGGAAGTCCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGTGCAGCTGTTGCACTTGACTTTGCAGTTAATCATCCGGAAGCTGTGGAAAAGCTGGTTTTGATCAATGCTTGTGTGTATAGCAACTGCACTGGGCATCCTACAAAGCTGCCGAAAGTGGTAGCATATGCTGGGGTTTCTTTGTTGAAAAGCATCCCATTGCGCCTCTTAGCAAATAAGTTAACCTTCAATGAACTTTCACTTGCTCGAAATGTGGAATGGACGAATGTTGGCCGTTTACATTGTCTATTGCCTTGGTGGGAAGATGCAACTGTTAATTTTATGTACAGCGGAGGCTACAATGTAGTTTCTCTGATTAAACAGGTCAAGCACAGAGCACTCATCATTGCAGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGAGGCTGCACTGTGAACTACCAAATGCAATAATGCGAATAATAACAGACAGTGGCCATCTGCCCCATGTTGACAGTCCTGCTTCTGTTTCAAAGCTTATTACAGAGTTTGTATTTCATGAATCCTGCTAG

Coding sequence (CDS)

ATGTCCGCGAAATGTGAAGGTCTTGGCATTGGAGATGTTTTCTCTGATGGAAATTGTGGCGGTTTTCCTTCGTTTCTTCCTAAAGAGATCACATTATCAGAAACTCGCCTTATGAGCAGCTGCGTTAAACCAGTAATAAGAAGCAAGAAGAGTCCAGTGGTTCTACTCCATTGCTTTGACAGTTCTTGTTTGGAATGGAGTTATGCGTTTCCTCTTCTTGAAGAGGCTGGACTAGAAACGTGGGCTGTTGATGTTCTTGGATGGGGCTTTTCTGACTTGGATAGTCTTCCTCCATGTAATGTGCAAACGAAGCGCTCTCATCTGTACCAGTTTTGGAAGTCCTACATCAAGAGGCCAATGGTGTTAGTTGGACCAAGCCTTGGTGCAGCTGTTGCACTTGACTTTGCAGTTAATCATCCGGAAGCTGTGGAAAAGCTGGTTTTGATCAATGCTTGTGTGTATAGCAACTGCACTGGGCATCCTACAAAGCTGCCGAAAGTGGTAGCATATGCTGGGGTTTCTTTGTTGAAAAGCATCCCATTGCGCCTCTTAGCAAATAAGTTAACCTTCAATGAACTTTCACTTGCTCGAAATGTGGAATGGACGAATGTTGGCCGTTTACATTGTCTATTGCCTTGGTGGGAAGATGCAACTGTTAATTTTATGTACAGCGGAGGCTACAATGTAGTTTCTCTGATTAAACAGGTCAAGCACAGAGCACTCATCATTGCAGGCGAAGATGACAGAATTATTCGCTATAAAGATGCAGTGAGGCTGCACTGTGAACTACCAAATGCAATAATGCGAATAATAACAGACAGTGGCCATCTGCCCCATGTTGACAGTCCTGCTTCTGTTTCAAAGCTTATTACAGAGTTTGTATTTCATGAATCCTGCTAG

Protein sequence

MSAKCEGLGIGDVFSDGNCGGFPSFLPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPASVSKLITEFVFHESC
Homology
BLAST of Csor.00g194060 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 64/250 (25.60%), Postives = 110/250 (44.00%), Query Frame = 0

Query: 50  KSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLY 109
           +  +V +H F SS   +    PLL +   +  A+D+  +G S+         Q     + 
Sbjct: 27  RQTLVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVI 86

Query: 110 QFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHP-----TKL 169
              +    +  VLVG S+G  ++L  A+  PE   K+VL+ +  Y     HP     T +
Sbjct: 87  GILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLK-RSHPTIIFGTHI 146

Query: 170 PKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYS 229
           P    Y    L K   ++ L N    ++ SL         GR       ++  T    + 
Sbjct: 147 PYFHLYIKRWLSKEGVMKNLLN--VVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHR 206

Query: 230 GGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPA 289
            G      +K++   AL+I GE+DRI+  +   RLH +LPN+++  +  +GHL   + P 
Sbjct: 207 EGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPE 266

Query: 290 SVSKLITEFV 295
            +S+ I +F+
Sbjct: 267 LISEHIADFI 272

BLAST of Csor.00g194060 vs. ExPASy Swiss-Prot
Match: A4JPX5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-08
Identity = 62/256 (24.22%), Postives = 105/256 (41.02%), Query Frame = 0

Query: 53  VVLLHCFDSSCLEWSYAFPLLE---EAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLY 112
           +VLLH        W+     ++    AG     VD  GWG SD               L 
Sbjct: 39  LVLLHGSGPGATGWANFHRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLA 98

Query: 113 QFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVA 172
               +       LVG S+G   A+ FA+++PE V KLVL+        TG P++   +  
Sbjct: 99  GVLDTLGIGRAHLVGNSMGGHSAVAFALSYPERVGKLVLM-----GGGTGGPSQFVPMPT 158

Query: 173 YAGVSLLKSI-------PLRLLANKLTFNELSLARNVEWTN----VGRLHCLLPWWEDAT 232
             G+ LL+++        L+ + N   ++  ++   +  T     +GR   L  + +  T
Sbjct: 159 -EGIKLLQALYRDPTLENLKKMLNVFVYDASTMTEELMQTRLENMLGRRDHLENFVKSLT 218

Query: 233 VNFMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLP 292
            N      Y     + ++K  AL+I G DDR +     +RL   +PNA + +    GH  
Sbjct: 219 ANPKQFPDYG--HRLSEIKAPALVIWGRDDRFVPMDVGLRLVWNMPNADLHVFGRCGHWA 278

Query: 293 HVDSPASVSKLITEFV 295
             +     ++++ EF+
Sbjct: 279 QWEHAERFNRMVLEFL 286

BLAST of Csor.00g194060 vs. ExPASy Swiss-Prot
Match: B2JQW2 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.7e-08
Identity = 66/265 (24.91%), Postives = 110/265 (41.51%), Query Frame = 0

Query: 50  KSPVVLLHCFDSSCLEWSYAFPLLE---EAGLETWAVDVLGWGFSD----LDSLPPCNVQ 109
           K  +VLLH        W+  +  ++    AG     VD  GWG SD      S    N +
Sbjct: 36  KETLVLLHGSGPGASGWANFYRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNAR 95

Query: 110 TKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPT 169
             +  L       I+R   LVG S+G   A+ FA+++PE V KLVL+        TG P+
Sbjct: 96  VLKGVLDTLG---IERAH-LVGNSMGGHSAVAFALSYPERVGKLVLM-----GGGTGGPS 155

Query: 170 KLPKVVAYAGVSLLKSI-------PLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWE 229
           +   +    G+ LL+ +        L+ + N   ++  ++   +  T +  +        
Sbjct: 156 QFVPMPT-EGIKLLQGLYRDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLAR----R 215

Query: 230 DATVNFMYSGGYNVVSL------IKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMR 289
           D   NF+ S   N          + ++K  AL+I G DDR +     +RL   LPNA   
Sbjct: 216 DHLENFVKSLTANPKQFPDYGHRLNEIKAPALVIWGRDDRFVPLDVGLRLVWGLPNAEFH 275

Query: 290 IITDSGHLPHVDSPASVSKLITEFV 295
           +    GH    +     ++++ +F+
Sbjct: 276 VFGRCGHWAQWEHAERFNQMLLDFL 286

BLAST of Csor.00g194060 vs. ExPASy Swiss-Prot
Match: P23974 (Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=menH PE=3 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 3.8e-08
Identity = 65/268 (24.25%), Postives = 111/268 (41.42%), Query Frame = 0

Query: 53  VVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKR-SHLYQF 112
           VV LH F  S   W++   +L ++ L    +D LG G +D          T++ S L + 
Sbjct: 27  VVCLHGFTGSKQSWTFLDEMLPDSRL--IKIDCLGHGETDAPLNGKRYSTTRQVSDLAEI 86

Query: 113 WKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTG-------HPTKL 172
           +       + L+G S+G  +A  FA+ +PE V  LVL +        G          KL
Sbjct: 87  FDQLKLHKVKLIGYSMGGRLAYSFAMTYPERVSALVLESTTPGLKTLGERRERIMRDRKL 146

Query: 173 PKVVAYAG----VSLLKSIPL-------------RLLANKLTFNELSLARNVEWTNVGRL 232
              +   G    V+  ++IPL             R+ + +L  N++ LA ++     G  
Sbjct: 147 ADFILRDGLEAFVAYWENIPLFSSQQRLAEDIRYRIRSGRLRNNKIGLANSLTGMGTGSQ 206

Query: 233 HCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRALIIAGE-DDRIIRYKDAVRLHCELPNA 292
             L   W                S ++++    L+I GE D++       V  H  LP++
Sbjct: 207 PSL---W----------------SRVEEIDVPVLLICGEWDEKFCAINQEV--HKMLPSS 266

Query: 293 IMRIITDSGHLPHVDSPASVSKLITEFV 295
            + I+  +GH  HV+ P    K+++EF+
Sbjct: 267 RIEIVPKAGHTVHVEQPRLFGKIVSEFL 271

BLAST of Csor.00g194060 vs. ExPASy Swiss-Prot
Match: P23133 (2-hydroxy-6-oxo-2,4-heptadienoate hydrolase OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=todF PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 3.8e-08
Identity = 59/260 (22.69%), Postives = 108/260 (41.54%), Query Frame = 0

Query: 51  SPVVLLHCFDSSCLEWSYAFPLLEEAG--LETWAVDVLGWGFSDLDSLPPCNVQTKRSHL 110
           +PVVL+H        W+    ++ E        A D++G+GF+         V++  +HL
Sbjct: 28  NPVVLVHGSGPGVTAWANWRTVMPELSRHRRVIAPDMVGFGFTQRPHGIHYGVESWVAHL 87

Query: 111 YQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYS--------NCTGH 170
                +     + LVG S G A++L FA+  P  V +LVL+ A   S           G+
Sbjct: 88  AGILDALELDRVDLVGNSFGGALSLAFAIRFPHRVRRLVLMGAVGVSFELTDGLDAVWGY 147

Query: 171 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVG---RLHCLLPWWEDA 230
              +P        ++ K +        L  +EL+  R    T  G       + P     
Sbjct: 148 EPSVP--------NMRKVMDYFAYDRSLVSDELAELRYKASTRPGFQEAFASMFPAPRQR 207

Query: 231 TVNFMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHL 290
            V+ + S   +    I+ ++H  LI+ G DDR+I  + ++RL+  +  + + +    GH 
Sbjct: 208 WVDALASSDQD----IRDIRHETLILHGRDDRVIPLETSLRLNQLIEPSQLHVFGRCGHW 267

Query: 291 PHVDSPASVSKLITEFVFHE 298
             ++      +L+ +F+  E
Sbjct: 268 VQIEQNRGFIRLVNDFLAAE 275

BLAST of Csor.00g194060 vs. NCBI nr
Match: KAG6607100.1 (hypothetical protein SDJN03_00442, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 618 bits (1593), Expect = 1.94e-223
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFD 60
           MSAKCEGLGIGDVFSDGNCGGFPSFLPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFD
Sbjct: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFD 60

Query: 61  SSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPM 120
           SSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPM
Sbjct: 61  SSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPM 120

Query: 121 VLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIP 180
           VLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIP
Sbjct: 121 VLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIP 180

Query: 181 LRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRA 240
           LRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRA
Sbjct: 181 LRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRA 240

Query: 241 LIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPASVSKLITEFVFHESC 299
           LIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPASVSKLITEFVFHESC
Sbjct: 241 LIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPASVSKLITEFVFHESC 299

BLAST of Csor.00g194060 vs. NCBI nr
Match: XP_022948386.1 (uncharacterized protein LOC111452078 isoform X2 [Cucurbita moschata])

HSP 1 Score: 602 bits (1553), Expect = 1.04e-216
Identity = 296/319 (92.79%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGIGDVFSDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 20  MSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 140 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNVVSLIKQVKHRALIIAGEDD+IIRYKDAVRLHCELPNAIMRI+TDSGHLPHV
Sbjct: 260 FMYSGGYNVVSLIKQVKHRALIIAGEDDKIIRYKDAVRLHCELPNAIMRIVTDSGHLPHV 319

BLAST of Csor.00g194060 vs. NCBI nr
Match: XP_022948385.1 (uncharacterized protein LOC111452078 isoform X1 [Cucurbita moschata])

HSP 1 Score: 602 bits (1553), Expect = 1.88e-216
Identity = 296/319 (92.79%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGIGDVFSDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 36  MSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 95

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 96  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 155

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 156 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 215

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN
Sbjct: 216 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 275

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNVVSLIKQVKHRALIIAGEDD+IIRYKDAVRLHCELPNAIMRI+TDSGHLPHV
Sbjct: 276 FMYSGGYNVVSLIKQVKHRALIIAGEDDKIIRYKDAVRLHCELPNAIMRIVTDSGHLPHV 335

BLAST of Csor.00g194060 vs. NCBI nr
Match: XP_023523746.1 (uncharacterized protein LOC111787885 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 598 bits (1542), Expect = 4.93e-215
Identity = 294/319 (92.16%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           +SAKCEGLGIGDVFSDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 20  LSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVK V RSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKSVTRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 140 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNVVSLIKQVKHRAL+IAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV
Sbjct: 260 FMYSGGYNVVSLIKQVKHRALVIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 319

BLAST of Csor.00g194060 vs. NCBI nr
Match: XP_022997786.1 (uncharacterized protein LOC111492638 isoform X2 [Cucurbita maxima])

HSP 1 Score: 597 bits (1539), Expect = 1.41e-214
Identity = 293/319 (91.85%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGI DV SDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 20  MSAKCEGLGIVDVLSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 140 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARN+EWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNLEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNV+SLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV
Sbjct: 260 FMYSGGYNVISLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 319

BLAST of Csor.00g194060 vs. ExPASy TrEMBL
Match: A0A6J1G926 (uncharacterized protein LOC111452078 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452078 PE=4 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 5.03e-217
Identity = 296/319 (92.79%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGIGDVFSDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 20  MSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 140 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNVVSLIKQVKHRALIIAGEDD+IIRYKDAVRLHCELPNAIMRI+TDSGHLPHV
Sbjct: 260 FMYSGGYNVVSLIKQVKHRALIIAGEDDKIIRYKDAVRLHCELPNAIMRIVTDSGHLPHV 319

BLAST of Csor.00g194060 vs. ExPASy TrEMBL
Match: A0A6J1G9P4 (uncharacterized protein LOC111452078 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452078 PE=4 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 9.09e-217
Identity = 296/319 (92.79%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGIGDVFSDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 36  MSAKCEGLGIGDVFSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 95

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 96  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 155

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 156 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 215

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN
Sbjct: 216 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 275

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNVVSLIKQVKHRALIIAGEDD+IIRYKDAVRLHCELPNAIMRI+TDSGHLPHV
Sbjct: 276 FMYSGGYNVVSLIKQVKHRALIIAGEDDKIIRYKDAVRLHCELPNAIMRIVTDSGHLPHV 335

BLAST of Csor.00g194060 vs. ExPASy TrEMBL
Match: A0A6J1K8F9 (uncharacterized protein LOC111492638 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492638 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 6.84e-215
Identity = 293/319 (91.85%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGI DV SDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 20  MSAKCEGLGIVDVLSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 140 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARN+EWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNLEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNV+SLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV
Sbjct: 260 FMYSGGYNVISLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 319

BLAST of Csor.00g194060 vs. ExPASy TrEMBL
Match: A0A6J1KAV9 (uncharacterized protein LOC111492638 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492638 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 1.24e-214
Identity = 293/319 (91.85%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKE--------------------ITLSETRLMSS 60
           MSAKCEGLGI DV SDGNCGGFPSFLPKE                    ITLSETRLMSS
Sbjct: 36  MSAKCEGLGIVDVLSDGNCGGFPSFLPKEVGKIKDPFARTMARKIERLPITLSETRLMSS 95

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 96  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 155

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH
Sbjct: 156 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 215

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARN+EWTNVGRLHCLLPWWEDATVN
Sbjct: 216 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNLEWTNVGRLHCLLPWWEDATVN 275

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FMYSGGYNV+SLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV
Sbjct: 276 FMYSGGYNVISLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 335

BLAST of Csor.00g194060 vs. ExPASy TrEMBL
Match: A0A6J1GTU8 (uncharacterized protein LOC111457484 OS=Cucurbita moschata OX=3662 GN=LOC111457484 PE=4 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 8.72e-202
Identity = 274/319 (85.89%), Postives = 290/319 (90.91%), Query Frame = 0

Query: 1   MSAKCEGLGIGDVFSDGNCGGFPSFLPKEI--------------------TLSETRLMSS 60
           M+AKCEGLG+G V SDGN G FPSFLPKE+                    TLSETR+MSS
Sbjct: 20  MAAKCEGLGLGGVLSDGNSGAFPSFLPKEVGKIKDPFARTMARKIERLPMTLSETRIMSS 79

Query: 61  CVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 120
           CVKPVIRS+K+PVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN
Sbjct: 80  CVKPVIRSEKNPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCN 139

Query: 121 VQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGH 180
           VQTKRSHLYQFWK+YIKRPMVLVGPSLGAAVA+DFAVNHPEAVEKLVLINACVYS CTGH
Sbjct: 140 VQTKRSHLYQFWKTYIKRPMVLVGPSLGAAVAVDFAVNHPEAVEKLVLINACVYSKCTGH 199

Query: 181 PTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVN 240
           PTKLPKVVAYAGVSLLKSIPLRL+ANKLTF+ LSLARN+EWTNVGRLHCLLPWWEDATVN
Sbjct: 200 PTKLPKVVAYAGVSLLKSIPLRLIANKLTFDGLSLARNLEWTNVGRLHCLLPWWEDATVN 259

Query: 241 FMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHV 299
           FM SGGYNV+S IKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIIT+SGHLPHV
Sbjct: 260 FMNSGGYNVISQIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITNSGHLPHV 319

BLAST of Csor.00g194060 vs. TAIR 10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 3.5e-105
Identity = 180/274 (65.69%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 26  LPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDV 85
           LP  ++  E  +MSSCV P++R++ +PVVLLH FDSSCLEW Y +PLLEEAGLETWA D+
Sbjct: 58  LPVSVSFREDSIMSSCVTPLMRNETTPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAFDI 117

Query: 86  LGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEK 145
           LGWGFSDLD LPPC+V +KR H Y+FWKS+IKRP+VLVGPSLGAAVA+D AVNHPEAVE 
Sbjct: 118 LGWGFSDLDKLPPCDVASKREHFYKFWKSHIKRPVVLVGPSLGAAVAIDIAVNHPEAVES 177

Query: 146 LVLINACVYSNCTGHPTKLPKVVAYAGVSLLKSIPLRLLANKLTFNELSLARNVEWTNVG 205
           LVL++A VY+  TG+   LPK  AYAGV LLKSIPLRL  N + FN +SL  + +WT +G
Sbjct: 178 LVLMDASVYAEGTGNLATLPKAAAYAGVYLLKSIPLRLYVNFICFNGISLETSWDWTKIG 237

Query: 206 RLHCLLPWWEDATVNFMYSGGYNVVSLIKQVKHRALIIAGEDDRIIRYKDAVRLHCELPN 265
           RLHCL PWWEDATV+FM SGGYNV SLIK+V  + LI+ GEDD+II  K A RLH EL N
Sbjct: 238 RLHCLYPWWEDATVSFMTSGGYNVTSLIKKVSQKTLILWGEDDQIISNKLAWRLHGELSN 297

Query: 266 AIMRIITDSGHLPHVDSPASVSKLITEFVFHESC 300
           A ++ I++ GHLPHV+ PA+V+KLI EFV  E+C
Sbjct: 298 ARVKQISNCGHLPHVEKPAAVTKLIAEFV-RETC 330

BLAST of Csor.00g194060 vs. TAIR 10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.5e-100
Identity = 172/301 (57.14%), Postives = 219/301 (72.76%), Query Frame = 0

Query: 18  NCGGFPSFLPKEI---------TLSET---------------RLMSSCVKPVIR-SKKSP 77
           N  GFP+FLPKEI          L++                 +MSSC+KP+++   KSP
Sbjct: 24  NGDGFPTFLPKEIQNIKDPFARALAQRIVRIPVPLQMGNFRGSVMSSCIKPLVQLHDKSP 83

Query: 78  VVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTKRSHLYQFW 137
           VVLLHCFDSSCLEW   +PLLE+A LETWA+DVLGWGFSDL+ LPPC+  +KR HL++ W
Sbjct: 84  VVLLHCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLPPCDAASKRHHLFELW 143

Query: 138 KSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINACVYSNCTGHPTKLPKVVAYAG 197
           K+YIKRPM+LVGPSLGA VA+DF   +PEAV+KLVLINA  YS  TG   +LPK +AYAG
Sbjct: 144 KTYIKRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEGTGRLKELPKSIAYAG 203

Query: 198 VSLLKSIPLRLLANKLTFNELSLARNVEWTNVGRLHCLLPWWEDATVNFMYSGGYNVVSL 257
           V LLKS PLRLLAN L F    L+ N++WTN+GRLHC +PWWEDA V+FM SGGYNV S 
Sbjct: 204 VKLLKSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDAMVDFMISGGYNVASH 263

Query: 258 IKQVKHRALIIAGEDDRIIRYKDAVRLHCELPNAIMRIITDSGHLPHVDSPASVSKLITE 294
           IK + H+ L++  E+D+I+  + +V+L CEL NA++R + DSGHLPHV++P  + KLI++
Sbjct: 264 IKHIDHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHLPHVENPKHIVKLISD 323

BLAST of Csor.00g194060 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-12
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 45  VIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTK 104
           V++ + SP+VL+H F +S   W Y  P L +   + +A+D+LG+G+SD  +L   +    
Sbjct: 37  VVQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVW 96

Query: 105 RSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINA 152
              +  F K  +K P V+VG SLG   AL  AV  PE V  + L+N+
Sbjct: 97  TDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNS 141

BLAST of Csor.00g194060 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-12
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 45  VIRSKKSPVVLLHCFDSSCLEWSYAFPLLEEAGLETWAVDVLGWGFSDLDSLPPCNVQTK 104
           V++ + SP+VL+H F +S   W Y  P L +   + +A+D+LG+G+SD  +L   +    
Sbjct: 94  VVQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVW 153

Query: 105 RSHLYQFWKSYIKRPMVLVGPSLGAAVALDFAVNHPEAVEKLVLINA 152
              +  F K  +K P V+VG SLG   AL  AV  PE V  + L+N+
Sbjct: 154 TDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNS 198

BLAST of Csor.00g194060 vs. TAIR 10
Match: AT1G64670.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 6.7e-08
Identity = 45/150 (30.00%), Postives = 69/150 (46.00%), Query Frame = 0

Query: 14  FSDGNCGGFPSFLPKEITLSETRLMSSCVKPVIRSKKSPVVLLHCFDSSCLEWSYA-FPL 73
           +SD +C    +F    ++ S   L  +  +P     +  VV +H F SS   W+   FP 
Sbjct: 150 WSDCHC----TFCTSWLSSSNQSLFVNVQQPTDNKAQENVVFIHGFLSSSTFWTETLFPN 209

Query: 74  LEEAGLETW---AVDVLGWGFSDLDSLPPCNVQTKRSHLYQFWKSYIK----RPMVLVGP 133
             ++    +   AVD+LG+G S     P  ++ T + HL    +S I     +   LV  
Sbjct: 210 FSDSAKSNYRFLAVDLLGYGKS---PKPNDSLYTLKEHLEMIERSVISQFRLKTFHLVAH 269

Query: 134 SLGAAVALDFAVNHPEAVEKLVLINACVYS 156
           SLG  +AL  AV HP A++ L L+    YS
Sbjct: 270 SLGCILALALAVKHPGAIKSLTLLAPPYYS 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O052351.7e-1125.60Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
A4JPX51.3e-0824.222-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burk... [more]
B2JQW21.7e-0824.912-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para... [more]
P239743.8e-0824.25Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase OS=Bacil... [more]
P231333.8e-0822.692-hydroxy-6-oxo-2,4-heptadienoate hydrolase OS=Pseudomonas putida (strain ATCC 7... [more]
Match NameE-valueIdentityDescription
KAG6607100.11.94e-223100.00hypothetical protein SDJN03_00442, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022948386.11.04e-21692.79uncharacterized protein LOC111452078 isoform X2 [Cucurbita moschata][more]
XP_022948385.11.88e-21692.79uncharacterized protein LOC111452078 isoform X1 [Cucurbita moschata][more]
XP_023523746.14.93e-21592.16uncharacterized protein LOC111787885 [Cucurbita pepo subsp. pepo][more]
XP_022997786.11.41e-21491.85uncharacterized protein LOC111492638 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G9265.03e-21792.79uncharacterized protein LOC111452078 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1G9P49.09e-21792.79uncharacterized protein LOC111452078 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K8F96.84e-21591.85uncharacterized protein LOC111492638 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KAV91.24e-21491.85uncharacterized protein LOC111492638 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GTU88.72e-20285.89uncharacterized protein LOC111457484 OS=Cucurbita moschata OX=3662 GN=LOC1114574... [more]
Match NameE-valueIdentityDescription
AT1G13820.13.5e-10565.69alpha/beta-Hydrolases superfamily protein [more]
AT5G39220.11.5e-10057.14alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.11.2e-1236.45alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.21.2e-1236.45alpha/beta-Hydrolases superfamily protein [more]
AT1G64670.16.7e-0830.00alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 271..293
score: 23.72
coord: 57..75
score: 27.99
coord: 136..149
score: 28.57
coord: 77..92
score: 35.8
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 122..135
score: 47.04
coord: 77..92
score: 34.05
coord: 240..254
score: 35.63
coord: 136..149
score: 41.87
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 40..297
e-value: 2.4E-36
score: 128.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 30..294
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 49..268
e-value: 2.9E-13
score: 49.6
NoneNo IPR availablePANTHERPTHR43689HYDROLASEcoord: 26..296
NoneNo IPR availablePANTHERPTHR43689:SF37ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 26..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g194060.m01Csor.00g194060.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity