Csor.00g182050 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g182050
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionheat stress transcription factor A-5-like
LocationCsor_Chr14: 15752056 .. 15754088 (-)
RNA-Seq ExpressionCsor.00g182050
SyntenyCsor.00g182050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCAGCTGCTGGCGGCGCTGGTCCGGCGCCGTTTCTCATCAAGACCTATGAAATGGTTGACGACTCCGCCACTGACGAGATCGTCTCATGGTCCTCTTCCAAGAACAGCTTTGTGGTCTGGAATCCCCCTGACTTCGCTCGCGTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAACTTAATACCTACGTAAGTTTCTGGAAATTCATCTTTCGCTCCGTGTTCTGATTTCGTCGTCTTCGTGATTTGAGTTTCTTCCTCCCGTTAATTGATCGTTGAGTATGTTCCTTACTGCGTAATAATGGTTTTATTGCTGTTTTTTCTCCCTCATTTACATAATATGCATATGTGTTCAAACATAATACATTCATCTCATGCGTTTTAGCGATTCTTAATTGTTCTGTATTCATTCCAATTCCAGTTGTTTCTGTATTTACGAGCTTAAATTGATGTAACTCGTAGGGATTTAACTAATTACATATCACGAGTTGCGTTGATAAACCAAATTTCGTTTGTTAATCATATTCTGTTACGCAATCTATTAGCTTCGAGATTCTCAAATCGCCTCCTTCTTTGGGAAGTTTTCTGAGAAGAATTTTCCAACAGATCTTTCTAGTTGAGATACTACAAGAAGAGAATTCTCTCCACTTACGCTTGCGCTCCCAATTTGTTATTACTCGATTCATATATCAAGAAATGGACCTTCGGAGGGAATGATTTTTCCATCTGTCATGCTAAAATCTCTTGAATTGCTACTGTTCTACACCTCATTTTACAAGAAAATTATATCATTCTTTCGTTTTGAAAAACTGAAGTGACTATGGGATTAAAAAGTAGCATGAACTTGAGAGGTTTGAAAATTTTGTTGGCAGGGCTTTCGAAAGAAAGATGCTGAGAGGTGGGAATTTGCCAATGAAGATTTCATAAAGGATAAAAAACATCTGCTGAAGAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCATGTAGATCCAGAGAGAGCTGCTTTTGAAGATGAAATAGGGAGGCTATCTAGTGAAAAAACAACAATTGAAGCTAATATCTCAAGGTTCAAACAGAAAAAATCGAGTGCTAAACTTCAGTTGCAAGAACTAACGATGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCTTTCTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTCTTCACAGCATTTAACAAAAAGAGACGACTGCCTTCTGCCGATCATTCACAGCCAGTCGTCGAAAACAGTTTTTTGGATAGCCATTGTAGTTCCAAATCTGAGCCTGGGATTATTTTTCATCAAGATTTCTCACATAAGCTGAGATTAGAAACTTCATGTGCATCAGATATTAACTTGATTTCATGTAGCACTCAGAGTTCAAATGAAGAAGGGGGAAGTTCACAACGAAATATGTCAAGAGCTGTCCAAGAACATCTTCATTTTGCAGCTGAAACATTAGATCTCTCAGACACTGGGGCATCGTTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAATGACAAGAGCCCACTTCTGCACTTATTGCAACCATATGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCCTGCCATTTGAATCTCACCCTGGCTTCTTCTTCGCTGCGAAACAACGACACTGCTTGTTCGGTTAGAACGCCACAACTGAGTCAGAATGTTCGAAAATCCCCAGATTCAAAGGTAGATTCAAATGCAAAAGAATCAGATATGAGACTGGGTTATCCCCAAGACACCTCAATGAACAATCATGGACCTCCGGCTGCTCCTATCAGGGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAGATTCTAACCACAGGGAAAATCTATACAAGGAGCCATATGATGGGAGATCAGGCCTATCTCTTTGA

mRNA sequence

ATGGAGGCAGCTGCTGGCGGCGCTGGTCCGGCGCCGTTTCTCATCAAGACCTATGAAATGGTTGACGACTCCGCCACTGACGAGATCGTCTCATGGTCCTCTTCCAAGAACAGCTTTGTGGTCTGGAATCCCCCTGACTTCGCTCGCGTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAACTTAATACCTACGGCTTTCGAAAGAAAGATGCTGAGAGGTGGGAATTTGCCAATGAAGATTTCATAAAGGATAAAAAACATCTGCTGAAGAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCATGTAGATCCAGAGAGAGCTGCTTTTGAAGATGAAATAGGGAGGCTATCTAGTGAAAAAACAACAATTGAAGCTAATATCTCAAGGTTCAAACAGAAAAAATCGAGTGCTAAACTTCAGTTGCAAGAACTAACGATGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCTTTCTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTCTTCACAGCATTTAACAAAAAGAGACGACTGCCTTCTGCCGATCATTCACAGCCAGTCGTCGAAAACAGTTTTTTGGATAGCCATTGTAGTTCCAAATCTGAGCCTGGGATTATTTTTCATCAAGATTTCTCACATAAGCTGAGATTAGAAACTTCATGTGCATCAGATATTAACTTGATTTCATGTAGCACTCAGAGTTCAAATGAAGAAGGGGGAAGTTCACAACGAAATATGTCAAGAGCTGTCCAAGAACATCTTCATTTTGCAGCTGAAACATTAGATCTCTCAGACACTGGGGCATCGTTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAATGACAAGAGCCCACTTCTGCACTTATTGCAACCATATGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCCTGCCATTTGAATCTCACCCTGGCTTCTTCTTCGCTGCGAAACAACGACACTGCTTGTTCGGTTAGAACGCCACAACTGAGTCAGAATGTTCGAAAATCCCCAGATTCAAAGGTAGATTCAAATGCAAAAGAATCAGATATGAGACTGGGTTATCCCCAAGACACCTCAATGAACAATCATGGACCTCCGGCTGCTCCTATCAGGGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAGATTCTAACCACAGGGAAAATCTATACAAGGAGCCATATGATGGGAGATCAGGCCTATCTCTTTGA

Coding sequence (CDS)

ATGGAGGCAGCTGCTGGCGGCGCTGGTCCGGCGCCGTTTCTCATCAAGACCTATGAAATGGTTGACGACTCCGCCACTGACGAGATCGTCTCATGGTCCTCTTCCAAGAACAGCTTTGTGGTCTGGAATCCCCCTGACTTCGCTCGCGTTCTTCTTCCTACGTTTTTCAAGCACAACAACTTCTCCAGCTTCATTCGGCAACTTAATACCTACGGCTTTCGAAAGAAAGATGCTGAGAGGTGGGAATTTGCCAATGAAGATTTCATAAAGGATAAAAAACATCTGCTGAAGAACATCCACCGTCGGAAACCAATTCACAGTCACAGTAATCCTCAAGGATCTCATGTAGATCCAGAGAGAGCTGCTTTTGAAGATGAAATAGGGAGGCTATCTAGTGAAAAAACAACAATTGAAGCTAATATCTCAAGGTTCAAACAGAAAAAATCGAGTGCTAAACTTCAGTTGCAAGAACTAACGATGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAATTTATTGGCTTTCTTAGAAAAGGCTGTTCAGAACCCTTCTTTTGTTGAACATCTTGCTCGAAGGGTCGAGTCTATGGACTTCTTCACAGCATTTAACAAAAAGAGACGACTGCCTTCTGCCGATCATTCACAGCCAGTCGTCGAAAACAGTTTTTTGGATAGCCATTGTAGTTCCAAATCTGAGCCTGGGATTATTTTTCATCAAGATTTCTCACATAAGCTGAGATTAGAAACTTCATGTGCATCAGATATTAACTTGATTTCATGTAGCACTCAGAGTTCAAATGAAGAAGGGGGAAGTTCACAACGAAATATGTCAAGAGCTGTCCAAGAACATCTTCATTTTGCAGCTGAAACATTAGATCTCTCAGACACTGGGGCATCGTTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAATGACAAGAGCCCACTTCTGCACTTATTGCAACCATATGTTAGTTCCAAAGAAGATGGAGAAAGTCACATCTCCTGCCATTTGAATCTCACCCTGGCTTCTTCTTCGCTGCGAAACAACGACACTGCTTGTTCGGTTAGAACGCCACAACTGAGTCAGAATGTTCGAAAATCCCCAGATTCAAAGGTAGATTCAAATGCAAAAGAATCAGATATGAGACTGGGTTATCCCCAAGACACCTCAATGAACAATCATGGACCTCCGGCTGCTCCTATCAGGGTCAATGATGTTTTCTGGGAACAGTTTCTTACCGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAGATTCTAACCACAGGGAAAATCTATACAAGGAGCCATATGATGGGAGATCAGGCCTATCTCTTTGA

Protein sequence

MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDGRSGLSL
Homology
BLAST of Csor.00g182050 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.5e-112
Identity = 236/454 (51.98%), Postives = 305/454 (67.18%), Query Frame = 0

Query: 6   GGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFI 65
           G  GPAPFL+KTYEMVDDS+TD+IVSWS++ NSF+VWN  +F+R+LLPT+FKHNNFSSFI
Sbjct: 17  GAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFKHNNFSSFI 76

Query: 66  RQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPERAAFED 125
           RQLNTYGFRK D ERWEF N+DFIKD+KHLLKNIHRRKPIHSHS+P  S  D ERA  ++
Sbjct: 77  RQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTDQERAVLQE 136

Query: 126 EIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQNPSF 185
           ++ +LS EK  IEA + +FKQ+K  AK Q +E+T  V+ ME RQK LL FLE A++NP+F
Sbjct: 137 QMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLETAIRNPTF 196

Query: 186 VEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLD-SHCSSKSEPGIIFHQDFSHK 245
           V++  ++VE +D  +A+NKKRRLP  + S+P  E+S LD S  SS+ E G IFHQ+FS+K
Sbjct: 197 VKNFGKKVEQLD-ISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIFHQNFSNK 256

Query: 246 LRLETSCA-SDINLISCSTQSSNEEGGS-------SQRNMSRAVQEHLHFAAETLDLSDT 305
           LRLE S A SD+N++S S QSSNEEG S          N +   +E L FA E L+L+DT
Sbjct: 257 LRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPEALELADT 316

Query: 306 GA--SFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNND 365
           G     +L  D++            +  LQ  ++S E+ +   SCHLNLTLAS+ L +  
Sbjct: 317 GTCPRRLLLNDNT-----------RVETLQQRLTSSEETDGSFSCHLNLTLASAPLPDKT 376

Query: 366 TACSVRTPQLSQ--NVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFW 425
            +   +T   SQ  N      S  + N    ++ +G     S  N  PPA   RVNDVFW
Sbjct: 377 ASQIAKTTLKSQELNFNSIETSASEKNRGRQEIAVG----GSQANAAPPA---RVNDVFW 436

Query: 426 EQFLTERPGCPESEEADSNHRENLYKEPYDGRSG 447
           EQFLTERPG  ++EEA S +R N Y+E  + R+G
Sbjct: 437 EQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of Csor.00g182050 vs. ExPASy Swiss-Prot
Match: Q6K6S5 (Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 5.8e-93
Identity = 218/468 (46.58%), Postives = 280/468 (59.83%), Query Frame = 0

Query: 3   AAAGGAGPAPFLIKTYEMVDDSATDEIVSWS-SSKNSFVVWNPPDFARVLLPTFFKHNNF 62
           A  GG GPAPFL+KTYEMVDD +TD +VSWS +S  SFVVWN P+FA  LLP +FKH+NF
Sbjct: 12  AGGGGGGPAPFLLKTYEMVDDPSTDAVVSWSDASDASFVVWNHPEFAARLLPAYFKHSNF 71

Query: 63  SSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPERA 122
           SSFIRQLNTYGFRK D ERWEFANE FIK +KHLLKNIHRRKPIHSHS+P G+  D ERA
Sbjct: 72  SSFIRQLNTYGFRKIDPERWEFANEYFIKGQKHLLKNIHRRKPIHSHSHPPGALPDNERA 131

Query: 123 AFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQ 182
            FEDEI RLS EK+ ++A++ + KQ++S    Q+++L  +V  ME+RQ  ++AFL++A +
Sbjct: 132 IFEDEIERLSREKSNLQADLWKSKQQQSGTMNQIEDLERRVLGMEQRQTKMIAFLQQASK 191

Query: 183 NPSFVEHLARRVESMDFFT-AFNKKRRLPSADHSQPVVE-NSFLDSHCS-SKSEPGIIFH 242
           NP FV  L +  E+   FT AFNKKRRLP  D+S    E  SF D H S SK E G + +
Sbjct: 192 NPQFVNKLVKMAEASSIFTDAFNKKRRLPGLDYSIENTETTSFYDDHSSTSKQETGNLLN 251

Query: 243 QDFSHKLRLETSCA-SDINLISCSTQSSNEEGGS---SQRNMSRAVQEHLHFAAETLDLS 302
           Q FS KLRL    A ++ N+I+ STQSSNE+  S            +E L    + ++LS
Sbjct: 252 QHFSDKLRLGLCPAMTESNIITLSTQSSNEDNRSPHGKHPECDMMGRECLPLVPQMMELS 311

Query: 303 DTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLR--- 362
           DTG S    + S  +               P +S     E  ++CHL+LTLAS S+    
Sbjct: 312 DTGTSICPSKSSCFA---------------PPISD----EGLLTCHLSLTLASCSMDVDK 371

Query: 363 -----------NNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGYP----QDTSMN 422
                      +N T  +  T +    + +S D   D+  K +D R         D  + 
Sbjct: 372 SQGLNANGTTIDNPTEAATATMEKDDTIDRSFD---DNQKKSADSRTADATTPRADARVA 431

Query: 423 NHGPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDGR 445
           +  P A    VND FWEQFLTERPGC E+EEA S  R +  +E  + R
Sbjct: 432 SEAPAAPAAVVNDKFWEQFLTERPGCSETEEASSGLRTDTSREQMENR 457

BLAST of Csor.00g182050 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.0e-53
Identity = 156/442 (35.29%), Postives = 225/442 (50.90%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           ME   GG    PFL KTYEMVDD +TD +V W+ +  SFVV N P+F R LLP +FKHNN
Sbjct: 1   MEGGGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQ---GSHVD 120
           FSSF+RQLNTYGFRK D E+WEFANEDFIK ++H LKNIHRRKPI SHS+     G   D
Sbjct: 61  FSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHSSHSQGAGPLTD 120

Query: 121 PERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLE 180
            ER  +E+EI RL S+   + + +     KK + + ++Q L  K+  +E +Q++L++++ 
Sbjct: 121 NERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRSLISYVR 180

Query: 181 KAVQNPSFVEHLARRVESMDFFTAFNKKRRLP---SADHSQPVVENSFLDSHCSSKSEPG 240
           + V+ P F   L+  V+  D      KKRRLP   S        EN  +   C   + P 
Sbjct: 181 EIVKAPGF---LSSFVQQQDH---HRKKRRLPIPISFHEDANTQENQIMP--CDLTNSPA 240

Query: 241 IIFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLS 300
             F+++   K+       S +N +    + ++EE G+                +    + 
Sbjct: 241 QTFYRESFDKME------SSLNSLENFLREASEEFGND--------------ISYDDGVP 300

Query: 301 DTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNND 360
              ++ +L    S     P   SP   +     SS   G+SH S             +  
Sbjct: 301 GPSSTVVLTELHSPGESDPRVSSPPTRM---RTSSAGAGDSHSS--------RDVAESTS 360

Query: 361 TACSVRTPQLSQNV-RKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWE 420
            A S   PQ+   V  ++  S++D N++ +    G  +D       PPA     ND FW+
Sbjct: 361 CAESPPIPQMHSRVDTRAKVSEIDVNSEPAVTETGPSRDQPAEE--PPAVTPGANDGFWQ 401

Query: 421 QFLTERPGCPES-EEADSNHRE 435
           QFLTE+PG  ++ +EA S  R+
Sbjct: 421 QFLTEQPGSSDAHQEAQSERRD 401

BLAST of Csor.00g182050 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.7e-53
Identity = 161/458 (35.15%), Postives = 227/458 (49.56%), Query Frame = 0

Query: 6   GGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFI 65
           GG GP PFLIKTYEMV+D+AT+ +VSW     SFVVWNP DF+R LLP +FKHNNFSSFI
Sbjct: 14  GGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNFSSFI 73

Query: 66  RQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHS---NPQGSHVDPERAA 125
           RQLNTYGFRK D ERWEFANEDFI+   HLLKNIHRRKP+HSHS      G   + ER  
Sbjct: 74  RQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQINGPLAESERRE 133

Query: 126 FEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQN 185
            E+EI RL  EK+ + A++ R  Q++     Q+Q +  ++ +ME+RQKN++A        
Sbjct: 134 LEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVA-------- 193

Query: 186 PSFVEHLARR----VESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFH 245
            S  E L RR      S+     F+KKRR+P  D         F+D  C++  E  +   
Sbjct: 194 -SLCEMLQRRGGAVSSSLLESDHFSKKRRVPKMD--------LFVDD-CAAGEEQKVFQF 253

Query: 246 QDFSHKLRL----------ETSCASDINLISCST--QSSNEEGGSSQRNMSR---AVQEH 305
           Q                  E     +++L+S     Q +N+   +++   S      +  
Sbjct: 254 QGIGTDAPAMPPVLPVTNGEAFDRVELSLVSLEKLFQRANDACTAAEEMYSHGHGGTEPS 313

Query: 306 LHFAAETLDLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNL 365
                E ++ +       L+  +SL   SPN  +  LHL      S    +S        
Sbjct: 314 TAICPEEMNTAPMETGIDLQLPASLHPSSPNTGNAHLHLSTELTESPGFVQSP------- 373

Query: 366 TLASSSLRNNDTACSVRT-PQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPA 425
            L  + +R +       T   ++  +  S D+  D  ++            +  +HGP  
Sbjct: 374 ELPMAEIREDIHVTRYPTQADVNSEIASSTDTSQDGTSE------------TEASHGP-- 428

Query: 426 APIRVNDVFWEQFLTERP-GCPESEEADSNHRENLYKE 440
                NDVFWE+FLTE P  C +  E   + ++++  E
Sbjct: 434 ----TNDVFWERFLTETPRSCLDESERQESPKDDVKAE 428

BLAST of Csor.00g182050 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 146/432 (33.80%), Postives = 213/432 (49.31%), Query Frame = 0

Query: 12  PFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLNTY 71
           PFL KTYEMVDDS++D IVSWS S  SF+VWNPP+F+R LLP FFKHNNFSSFIRQLNTY
Sbjct: 15  PFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSFIRQLNTY 74

Query: 72  GFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQ-----GSHVDPERAAFEDE 131
           GFRK D E+WEFAN+DF++ + HL+KNIHRRKP+HSHS P          D ER    ++
Sbjct: 75  GFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDSERVRMNNQ 134

Query: 132 IGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQNPSFV 191
           I RL+ EK  +   + +  +++   ++Q++EL  +++ MEKRQK +++F+ + ++ P   
Sbjct: 135 IERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQVLEKPGLA 194

Query: 192 EHLARRVESMDFFTAFNKKRRLPSADH--SQPVVENSFLDSHCSSKSEPGIIFHQDFSHK 251
            +L+  V   +      +KRR P  +    +P++E +        +       H      
Sbjct: 195 LNLSPCVPETN-----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSSHTREHQV 254

Query: 252 LRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASFILKR 311
            +LE+S A   NL+S S +S  +         SR++         TLD+ ++        
Sbjct: 255 EQLESSIAIWENLVSDSCESMLQ---------SRSMM--------TLDVDES-------- 314

Query: 312 DSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVRTPQL 371
                  S   +SP L  +Q  V S+               +  S R  D  C       
Sbjct: 315 -------STFPESPPLSCIQLSVDSR-------------LKSPPSPRIIDMNC------- 367

Query: 372 SQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIR-VNDVFWEQFLTERPGCP 431
                  PD   + N   +                PP  P+   ND FW+QF +E PG  
Sbjct: 375 ------EPDGSKEQNTVAA----------------PPPPPVAGANDGFWQQFFSENPGST 367

Query: 432 ESEEADSNHREN 436
           E  E     +++
Sbjct: 435 EQREVQLERKDD 367

BLAST of Csor.00g182050 vs. NCBI nr
Match: KAG6582493.1 (Heat stress transcription factor A-5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018877.1 Heat stress transcription factor A-5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 890 bits (2300), Expect = 0.0
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD
Sbjct: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR
Sbjct: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEADSNHRENLYKEPYDGRSGLSL
Sbjct: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449

BLAST of Csor.00g182050 vs. NCBI nr
Match: XP_022924244.1 (heat stress transcription factor A-5-like [Cucurbita moschata] >XP_022924245.1 heat stress transcription factor A-5-like [Cucurbita moschata])

HSP 1 Score: 877 bits (2267), Expect = 0.0
Identity = 446/449 (99.33%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVESMDF TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD
Sbjct: 181 QNPSFVEHLARRVESMDF-TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR
Sbjct: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQ SQNVRKSPDSKVDSNAKESDMRLG PQDTSMNNHGPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQQSQNVRKSPDSKVDSNAKESDMRLGCPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEADSNHRENLYKEPYDGRSGLSL
Sbjct: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 448

BLAST of Csor.00g182050 vs. NCBI nr
Match: XP_023526227.1 (heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo] >XP_023526228.1 heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo] >XP_023526229.1 heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo] >XP_023526230.1 heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865 bits (2235), Expect = 0.0
Identity = 439/449 (97.77%), Postives = 443/449 (98.66%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPP+FARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPEFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVESMDF TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPG IFHQD
Sbjct: 181 QNPSFVEHLARRVESMDF-TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGNIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSL+GKSPNDKSPLLHLLQPYVSSKEDG+SHISCHLNLTLASSSLRNNDTACSVR
Sbjct: 301 ILKRDSSLTGKSPNDKSPLLHLLQPYVSSKEDGDSHISCHLNLTLASSSLRNNDTACSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQL QNVRKSPDSKV SN KESD+RLG PQDTSMNNHGPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQLDQNVRKSPDSKVISNGKESDIRLGCPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEADSNHRENLYKEPYDGRSGLSL
Sbjct: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 448

BLAST of Csor.00g182050 vs. NCBI nr
Match: XP_022980292.1 (heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022980293.1 heat stress transcription factor A-5-like [Cucurbita maxima])

HSP 1 Score: 850 bits (2196), Expect = 2.78e-310
Identity = 433/449 (96.44%), Postives = 439/449 (97.77%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPP+FARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPEFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKD+KHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDEKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVES+EKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESVEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVES DF TAFNKKRRLPSADHSQPV+ENSFLDSHCSSKSEPG IFHQD
Sbjct: 181 QNPSFVEHLARRVESKDF-TAFNKKRRLPSADHSQPVIENSFLDSHCSSKSEPGNIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDT+CSVR
Sbjct: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTSCSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQLSQNVRKSPDSKV SN KESDMRLG PQDTS NN GPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQLSQNVRKSPDSKVTSNGKESDMRLGCPQDTSKNNRGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEA+SNHRENLYKE  DGRSGLSL
Sbjct: 421 GCPESEEANSNHRENLYKETDDGRSGLSL 448

BLAST of Csor.00g182050 vs. NCBI nr
Match: XP_022948442.1 (heat stress transcription factor A-5-like [Cucurbita moschata])

HSP 1 Score: 726 bits (1874), Expect = 7.06e-261
Identity = 382/471 (81.10%), Postives = 411/471 (87.26%), Query Frame = 0

Query: 1   MEAAAGG-----AGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTF 60
           MEAAAGG     AGPAPFLIKTY+MVD+SATDEIVSW+SSK SFVVWNPP+FAR+LLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHNNFSSFIRQLNTYGF+K D+ERWEFAN+DFIKD+KHLLKNIHRRKPIHSHS P GS 
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAF 180
            DPERAAFEDEI RLS +KTT+EA+ISRFKQ+KS+AK+QLQ+LT+KVESMEKRQKNLLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGI 240
           LEKAVQNPSFVEHLARRVESMDF TAFNKKRRLPSADHSQPV+ENSFLD+H SS+S  G 
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDF-TAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGN 240

Query: 241 IFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMS----RAVQEHLHFAAETL 300
           IFHQDFSHK RLETS  SDINL+SCSTQSSNE GGSSQRNMS    R VQE  HFAAETL
Sbjct: 241 IFHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETL 300

Query: 301 DLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLR 360
           DLSDTG SF+LKRDSSLSGKSPND+SP LH LQP VSSKEDGESHISCHLNLTLASSSLR
Sbjct: 301 DLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLR 360

Query: 361 NNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGYP-------------QDTSMNNH 420
            ND  CSVR PQLSQN+RKS  SKV+SN KESDMRL                ++TS NNH
Sbjct: 361 VNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNH 420

Query: 421 GPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GPPA P+RVNDVFWEQFLTERPGCPESEEA SN+RENLYKEP DGRSGLSL
Sbjct: 421 GPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 470

BLAST of Csor.00g182050 vs. ExPASy TrEMBL
Match: A0A6J1E8L0 (heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC111431772 PE=3 SV=1)

HSP 1 Score: 877 bits (2267), Expect = 0.0
Identity = 446/449 (99.33%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVESMDF TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD
Sbjct: 181 QNPSFVEHLARRVESMDF-TAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR
Sbjct: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQ SQNVRKSPDSKVDSNAKESDMRLG PQDTSMNNHGPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQQSQNVRKSPDSKVDSNAKESDMRLGCPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEADSNHRENLYKEPYDGRSGLSL
Sbjct: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 448

BLAST of Csor.00g182050 vs. ExPASy TrEMBL
Match: A0A6J1IYV8 (heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC111479710 PE=3 SV=1)

HSP 1 Score: 850 bits (2196), Expect = 1.34e-310
Identity = 433/449 (96.44%), Postives = 439/449 (97.77%), Query Frame = 0

Query: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNN 60
           MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPP+FARVLLPTFFKHNN
Sbjct: 1   MEAAAGGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPEFARVLLPTFFKHNN 60

Query: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPER 120
           FSSFIRQLNTYGFRKKDAERWEFANEDFIKD+KHLLKNIHRRKPIHSHSNPQGSHVDPER
Sbjct: 61  FSSFIRQLNTYGFRKKDAERWEFANEDFIKDEKHLLKNIHRRKPIHSHSNPQGSHVDPER 120

Query: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAV 180
           AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVES+EKRQKNLLAFLEKAV
Sbjct: 121 AAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESVEKRQKNLLAFLEKAV 180

Query: 181 QNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGIIFHQD 240
           QNPSFVEHLARRVES DF TAFNKKRRLPSADHSQPV+ENSFLDSHCSSKSEPG IFHQD
Sbjct: 181 QNPSFVEHLARRVESKDF-TAFNKKRRLPSADHSQPVIENSFLDSHCSSKSEPGNIFHQD 240

Query: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300
           FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF
Sbjct: 241 FSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASF 300

Query: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVR 360
           ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDT+CSVR
Sbjct: 301 ILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTSCSVR 360

Query: 361 TPQLSQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFWEQFLTERP 420
           TPQLSQNVRKSPDSKV SN KESDMRLG PQDTS NN GPPAAPIRVNDVFWEQFLTERP
Sbjct: 361 TPQLSQNVRKSPDSKVTSNGKESDMRLGCPQDTSKNNRGPPAAPIRVNDVFWEQFLTERP 420

Query: 421 GCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GCPESEEA+SNHRENLYKE  DGRSGLSL
Sbjct: 421 GCPESEEANSNHRENLYKETDDGRSGLSL 448

BLAST of Csor.00g182050 vs. ExPASy TrEMBL
Match: A0A6J1G983 (heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC111452123 PE=3 SV=1)

HSP 1 Score: 726 bits (1874), Expect = 3.42e-261
Identity = 382/471 (81.10%), Postives = 411/471 (87.26%), Query Frame = 0

Query: 1   MEAAAGG-----AGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTF 60
           MEAAAGG     AGPAPFLIKTY+MVD+SATDEIVSW+SSK SFVVWNPP+FAR+LLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHNNFSSFIRQLNTYGF+K D+ERWEFAN+DFIKD+KHLLKNIHRRKPIHSHS P GS 
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAF 180
            DPERAAFEDEI RLS +KTT+EA+ISRFKQ+KS+AK+QLQ+LT+KVESMEKRQKNLLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGI 240
           LEKAVQNPSFVEHLARRVESMDF TAFNKKRRLPSADHSQPV+ENSFLD+H SS+S  G 
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDF-TAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGN 240

Query: 241 IFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMS----RAVQEHLHFAAETL 300
           IFHQDFSHK RLETS  SDINL+SCSTQSSNE GGSSQRNMS    R VQE  HFAAETL
Sbjct: 241 IFHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETL 300

Query: 301 DLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLR 360
           DLSDTG SF+LKRDSSLSGKSPND+SP LH LQP VSSKEDGESHISCHLNLTLASSSLR
Sbjct: 301 DLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLR 360

Query: 361 NNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGYP-------------QDTSMNNH 420
            ND  CSVR PQLSQN+RKS  SKV+SN KESDMRL                ++TS NNH
Sbjct: 361 VNDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNH 420

Query: 421 GPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GPPA P+RVNDVFWEQFLTERPGCPESEEA SN+RENLYKEP DGRSGLSL
Sbjct: 421 GPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 470

BLAST of Csor.00g182050 vs. ExPASy TrEMBL
Match: A0A6J1IB49 (heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC111473483 PE=3 SV=1)

HSP 1 Score: 717 bits (1851), Expect = 1.04e-257
Identity = 378/471 (80.25%), Postives = 409/471 (86.84%), Query Frame = 0

Query: 1   MEAAAGG-----AGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTF 60
           MEAAAGG     AGPAPFLIKTY+MVD+SATDEIVSW+SSK SFVVWNPP+FAR+LLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSH 120
           FKHNNFSSFIRQLNTYGF+K D+ERWEFAN+DFIKD+KHLLKNIHRRKPIHSHS P GS 
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAF 180
           VDPERAAFEDEI RLS +KTT+EA+ISRFKQ+KS+AK+QLQ+LT+KVESMEKRQKNLLAF
Sbjct: 121 VDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGI 240
           LEKAVQNPSFVEHLARRVESMDF TAFNKKRRLPS DHSQP +ENSFLD+H SS+SE G 
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDF-TAFNKKRRLPSGDHSQPAIENSFLDNHSSSRSESGN 240

Query: 241 IFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMS----RAVQEHLHFAAETL 300
           IFHQDFSHK+RLETS  SDINL+SCSTQSSNE GGSSQRNMS    R VQE  HFAAETL
Sbjct: 241 IFHQDFSHKMRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETL 300

Query: 301 DLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLR 360
           DLSDTG SF+LKRDSSLSGKSPND+SP LH LQP VSSKEDGE+HISCHLNLTLASSSL+
Sbjct: 301 DLSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGENHISCHLNLTLASSSLQ 360

Query: 361 NNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGYP-------------QDTSMNNH 420
            ND   SVR PQL QN+RKS  SKV SN KESDMRL                ++TS NNH
Sbjct: 361 VNDADYSVRMPQLGQNLRKSSGSKV-SNGKESDMRLFTKNINLDEGTSTFCLKETSSNNH 420

Query: 421 GPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDGRSGLSL 449
           GPPA P+RVNDVFWEQFLTERPGCPESEEA SN+RENLYKEP DGRSGLSL
Sbjct: 421 GPPANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 469

BLAST of Csor.00g182050 vs. ExPASy TrEMBL
Match: A0A1S3AWR2 (heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=3 SV=1)

HSP 1 Score: 715 bits (1845), Expect = 7.95e-257
Identity = 377/465 (81.08%), Postives = 405/465 (87.10%), Query Frame = 0

Query: 1   MEAAAGG-----AGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTF 60
           MEAAAGG     AGPAPFL+KTY+MVDDS+TDEIVSW+SSK SFVVWNPP+FAR+LLPTF
Sbjct: 1   MEAAAGGGGTGGAGPAPFLLKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSH 120
           FKH+NFSSFIRQLNTYGFRK D+E+WEFANEDFIKD+KHLLKNIHRRKPIHSHSNPQGSH
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 VDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAF 180
           +DPERAAFEDEI RLS EK T+E NISRFKQ+KS+AKLQLQ+LT+KVESMEKRQKNLLAF
Sbjct: 121 IDPERAAFEDEIERLSREKNTLEVNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSKSEPGI 240
           LEKAVQNPSFVEHLARRVESMDF TAF KKRRLPSAD SQPV+ENSFLD+H SS+SE G 
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDF-TAFKKKRRLPSADLSQPVIENSFLDNHSSSRSESGN 240

Query: 241 IFHQDFSHKLRLETSCASDINLISCSTQSSNEEGGSSQRNMS----RAVQEHLHFAAETL 300
           IFHQDFS KLRLETSCASDINLIS STQSSNEEGGSSQR +S    RAVQE+LHFAAETL
Sbjct: 241 IFHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENLHFAAETL 300

Query: 301 DLSDTGASFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLR 360
           DLSDTG SFIL+RDSSLSGKS ND +P LH LQP VSSKEDGESHISC LNLTLASSSLR
Sbjct: 301 DLSDTGTSFILRRDSSLSGKSHNDDNPRLHSLQPSVSSKEDGESHISCQLNLTLASSSLR 360

Query: 361 NNDTACSVRTPQLSQNVRKSPDSKVDSNAKESDMRLGY-------------PQDTSMNNH 420
            NDTACSVR PQL QNVRK PDSK +SN KESD+RL               PQ+TS NNH
Sbjct: 361 INDTACSVRMPQLGQNVRKFPDSKANSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNH 420

Query: 421 GPPAAPIRVNDVFWEQFLTERPGCPESEEADSNHRENLYKEPYDG 443
           GPPAA IR NDVFWE+ LTERPGCPESEEA SN+R N YKEP DG
Sbjct: 421 GPPAASIRANDVFWERLLTERPGCPESEEASSNYRANPYKEPDDG 464

BLAST of Csor.00g182050 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.8e-113
Identity = 236/454 (51.98%), Postives = 305/454 (67.18%), Query Frame = 0

Query: 6   GGAGPAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFI 65
           G  GPAPFL+KTYEMVDDS+TD+IVSWS++ NSF+VWN  +F+R+LLPT+FKHNNFSSFI
Sbjct: 17  GAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFKHNNFSSFI 76

Query: 66  RQLNTYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQGSHVDPERAAFED 125
           RQLNTYGFRK D ERWEF N+DFIKD+KHLLKNIHRRKPIHSHS+P  S  D ERA  ++
Sbjct: 77  RQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTDQERAVLQE 136

Query: 126 EIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQNPSF 185
           ++ +LS EK  IEA + +FKQ+K  AK Q +E+T  V+ ME RQK LL FLE A++NP+F
Sbjct: 137 QMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLETAIRNPTF 196

Query: 186 VEHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLD-SHCSSKSEPGIIFHQDFSHK 245
           V++  ++VE +D  +A+NKKRRLP  + S+P  E+S LD S  SS+ E G IFHQ+FS+K
Sbjct: 197 VKNFGKKVEQLD-ISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIFHQNFSNK 256

Query: 246 LRLETSCA-SDINLISCSTQSSNEEGGS-------SQRNMSRAVQEHLHFAAETLDLSDT 305
           LRLE S A SD+N++S S QSSNEEG S          N +   +E L FA E L+L+DT
Sbjct: 257 LRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPEALELADT 316

Query: 306 GA--SFILKRDSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNND 365
           G     +L  D++            +  LQ  ++S E+ +   SCHLNLTLAS+ L +  
Sbjct: 317 GTCPRRLLLNDNT-----------RVETLQQRLTSSEETDGSFSCHLNLTLASAPLPDKT 376

Query: 366 TACSVRTPQLSQ--NVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIRVNDVFW 425
            +   +T   SQ  N      S  + N    ++ +G     S  N  PPA   RVNDVFW
Sbjct: 377 ASQIAKTTLKSQELNFNSIETSASEKNRGRQEIAVG----GSQANAAPPA---RVNDVFW 436

Query: 426 EQFLTERPGCPESEEADSNHRENLYKEPYDGRSG 447
           EQFLTERPG  ++EEA S +R N Y+E  + R+G
Sbjct: 437 EQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of Csor.00g182050 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 204.9 bits (520), Expect = 1.3e-52
Identity = 146/432 (33.80%), Postives = 213/432 (49.31%), Query Frame = 0

Query: 12  PFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLNTY 71
           PFL KTYEMVDDS++D IVSWS S  SF+VWNPP+F+R LLP FFKHNNFSSFIRQLNTY
Sbjct: 15  PFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSFIRQLNTY 74

Query: 72  GFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQ-----GSHVDPERAAFEDE 131
           GFRK D E+WEFAN+DF++ + HL+KNIHRRKP+HSHS P          D ER    ++
Sbjct: 75  GFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDSERVRMNNQ 134

Query: 132 IGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQNPSFV 191
           I RL+ EK  +   + +  +++   ++Q++EL  +++ MEKRQK +++F+ + ++ P   
Sbjct: 135 IERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQVLEKPGLA 194

Query: 192 EHLARRVESMDFFTAFNKKRRLPSADH--SQPVVENSFLDSHCSSKSEPGIIFHQDFSHK 251
            +L+  V   +      +KRR P  +    +P++E +        +       H      
Sbjct: 195 LNLSPCVPETN-----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSSHTREHQV 254

Query: 252 LRLETSCASDINLISCSTQSSNEEGGSSQRNMSRAVQEHLHFAAETLDLSDTGASFILKR 311
            +LE+S A   NL+S S +S  +         SR++         TLD+ ++        
Sbjct: 255 EQLESSIAIWENLVSDSCESMLQ---------SRSMM--------TLDVDES-------- 314

Query: 312 DSSLSGKSPNDKSPLLHLLQPYVSSKEDGESHISCHLNLTLASSSLRNNDTACSVRTPQL 371
                  S   +SP L  +Q  V S+               +  S R  D  C       
Sbjct: 315 -------STFPESPPLSCIQLSVDSR-------------LKSPPSPRIIDMNC------- 367

Query: 372 SQNVRKSPDSKVDSNAKESDMRLGYPQDTSMNNHGPPAAPIR-VNDVFWEQFLTERPGCP 431
                  PD   + N   +                PP  P+   ND FW+QF +E PG  
Sbjct: 375 ------EPDGSKEQNTVAA----------------PPPPPVAGANDGFWQQFFSENPGST 367

Query: 432 ESEEADSNHREN 436
           E  E     +++
Sbjct: 435 EQREVQLERKDD 367

BLAST of Csor.00g182050 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 199.1 bits (505), Expect = 7.4e-51
Identity = 103/209 (49.28%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 12  PFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLNTY 71
           PFL KTY+MVDD  T+E+VSWSS  NSFVVW+ P+F++VLLP +FKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 72  GFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQ---------GSHVDPERAA 131
           GFRK D +RWEFANE F++ +K LLK+I RRKP H   N Q         G+ V+  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 132 FEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQN 191
            E+E+ RL  +K  +   + R +Q++ + + QLQ +  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 192 PSFVEHLARR--VESMDFFTAFNKKRRLP 210
           P F+  L ++   +        NKKRRLP
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLP 235

BLAST of Csor.00g182050 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 199.1 bits (505), Expect = 7.4e-51
Identity = 103/209 (49.28%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 12  PFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLNTY 71
           PFL KTY+MVDD  T+E+VSWSS  NSFVVW+ P+F++VLLP +FKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 72  GFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSHSNPQ---------GSHVDPERAA 131
           GFRK D +RWEFANE F++ +K LLK+I RRKP H   N Q         G+ V+  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 132 FEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKNLLAFLEKAVQN 191
            E+E+ RL  +K  +   + R +Q++ + + QLQ +  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 192 PSFVEHLARR--VESMDFFTAFNKKRRLP 210
           P F+  L ++   +        NKKRRLP
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLP 235

BLAST of Csor.00g182050 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 197.6 bits (501), Expect = 2.1e-50
Identity = 111/249 (44.58%), Postives = 157/249 (63.05%), Query Frame = 0

Query: 10  PAPFLIKTYEMVDDSATDEIVSWSSSKNSFVVWNPPDFARVLLPTFFKHNNFSSFIRQLN 69
           P PFL KTY+MV+D ATD IVSWS + NSF+VW+PP+F+R LLP +FKHNNFSSF+RQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 70  TYGFRKKDAERWEFANEDFIKDKKHLLKNIHRRKPIHSH----SNPQ------------- 129
           TYGFRK D +RWEFANE F++ +KHLLK I RRK +  H    SNPQ             
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 130 -GSHVDPERAAFEDEIGRLSSEKTTIEANISRFKQKKSSAKLQLQELTMKVESMEKRQKN 189
             S V+  +   E+E+ +L  +K  +   + + +Q++ +   +LQ L   ++ ME+RQ+ 
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

Query: 190 LLAFLEKAVQNPSFV-EHLARRVESMDFFTAFNKKRRLPSADHSQPVVENSFLDSHCSSK 240
           +++FL KAVQNP+F+ + + ++ +S    T  NKKRRL   D +     NS   SH    
Sbjct: 230 IMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRL-REDSTAATESNS--HSHSLEA 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94BZ52.5e-11251.98Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE... [more]
Q6K6S55.8e-9346.58Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q94J162.0e-5335.29Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q93VB57.7e-5335.15Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O494031.9e-5133.80Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
Match NameE-valueIdentityDescription
KAG6582493.10.0100.00Heat stress transcription factor A-5, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022924244.10.099.33heat stress transcription factor A-5-like [Cucurbita moschata] >XP_022924245.1 h... [more]
XP_023526227.10.097.77heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo] >XP_02352... [more]
XP_022980292.12.78e-31096.44heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022980293.1 hea... [more]
XP_022948442.17.06e-26181.10heat stress transcription factor A-5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1E8L00.099.33heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IYV81.34e-31096.44heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1G9833.42e-26181.10heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IB491.04e-25780.25heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3AWR27.95e-25781.08heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=... [more]
Match NameE-valueIdentityDescription
AT4G13980.11.8e-11351.98winged-helix DNA-binding transcription factor family protein [more]
AT4G18880.11.3e-5233.80heat shock transcription factor A4A [more]
AT5G16820.17.4e-5149.28heat shock factor 3 [more]
AT5G16820.27.4e-5149.28heat shock factor 3 [more]
AT4G17750.12.1e-5044.58heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 141..168
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 419..449
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..402
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 420..440
NoneNo IPR availablePANTHERPTHR10015:SF377HEAT STRESS TRANSCRIPTION FACTOR A-5coord: 6..445
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 13..36
score: 45.36
coord: 51..63
score: 72.97
coord: 64..76
score: 79.56
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 9..102
e-value: 1.3E-56
score: 204.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 13..102
e-value: 5.6E-30
score: 103.8
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 7..103
e-value: 5.2E-35
score: 122.0
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 6..445
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 9..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g182050.m01Csor.00g182050.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0043565 sequence-specific DNA binding