Csor.00g174210 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g174210
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Alpha-amylase
Location	Csor_Chr09: 1015327 .. 1016868 (-)
RNA-Seq Expression	Csor.00g174210
Synteny	Csor.00g174210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTCCTAGCTTCTCTGTGCTTCCTCTGCTACCTCTCTGTTTTCCTCTTCCCATCCCCCTCCTTTTCTTCTACCTTACTGTTTCAGGTACGCATATTCATCAACCAATATTTGCAATTATCATGTAAGACTTACTTTTAAAAAGCTTGAAATTGATGGTGTAATGAAACAGGGCTTTAACTGGGAATCGAGTAGCAAAGGAGGATGGTACAACTCACTGAACAACATAATTCCTGATCTGGTACATGCTAAAGTTACTCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTTCTCCACAAGGCAAGCCTTCTTCCTCTGTTGCCTTTCACTTGAAATCTAAATTTGTGTTGCTCAAACCCAAAACCCTCTTACAGGATACTTACCCGGAAGGCTATATGATCTGAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTCATCGCCGCCCTCCACAATAATGGAATCAAGGCCGTAGCCGACATAGTGATCAACCACAGAACAGCAGATAAACAGGACGAGAGAGGAATTTGGTGCATTTTTGAGGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATGCAGCAGCACCTGATATCGACCATCTCAACCCACAGGTTCAGAATGAATTATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGACTTTGTAAAGGGCTATGCACCAAAAATTACAAAACTCTACATGGATCAAACCTCGCCAGATTTTGCAGTCGGTGAGAAATGGGATTCACTTTCATCTGGACCGGACGGGAAGCCCGATCCAAACCAAGACGGGCATCGCGAAGCTCTTGCAAACTGGGTTAGGGATTCAGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGTATTCTTCAAGCTGCCGTTCAGGAGGAGCTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACCTTCATCGACAACCACGACACTGGTTCGACGCAAAAACTCTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATATGCATACATTCTCACGCATCCTGGAACGCCCTCCATTGTAATCCATTCAACAATACGGTTTAGGTTTTTGAGTTTCGAAACTTAATACCTGTAATTCACGTAGTGTAGGCTTGAACATCTGTTTGTTTGTTTGTTGCAGTTCTACGACCATTTCGTTGAATGGGGATTGAAGGAGGAGCTAATCAAATTGAGCGCAATCAGGGCAAGGAATGGGATTGGGGAAAGGAGCACGGTGAATATTATGGCTTCTGACGGCGATCTATACGTTGCTGGCATTGATGGGAAAATTATAATGAAGATCGGACCGAACACCAATCTTGGAAATCTGGTTCCGTCGGATTATGAGCTTGCCGCTTCTGGAATGCAGTATGCTGTCTGGGAGAAAAAGAAATGA

mRNA sequence

ATGGGTTTCCTAGCTTCTCTGTGCTTCCTCTGCTACCTCTCTGTTTTCCTCTTCCCATCCCCCTCCTTTTCTTCTACCTTACTGTTTCAGGGCTTTAACTGGGAATCGAGTAGCAAAGGAGGATGGTACAACTCACTGAACAACATAATTCCTGATCTGGTACATGCTAAAGTTACTCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTTCTCCACAAGGATACTTACCCGGAAGGCTATATGATCTGAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTCATCGCCGCCCTCCACAATAATGGAATCAAGGCCGTAGCCGACATAGTGATCAACCACAGAACAGCAGATAAACAGGACGAGAGAGGAATTTGGTGCATTTTTGAGGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATGCAGCAGCACCTGATATCGACCATCTCAACCCACAGGTTCAGAATGAATTATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGACTTTGTAAAGGGCTATGCACCAAAAATTACAAAACTCTACATGGATCAAACCTCGCCAGATTTTGCAGTCGGTGAGAAATGGGATTCACTTTCATCTGGACCGGACGGGAAGCCCGATCCAAACCAAGACGGGCATCGCGAAGCTCTTGCAAACTGGGTTAGGGATTCAGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGTATTCTTCAAGCTGCCGTTCAGGAGGAGCTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACCTTCATCGACAACCACGACACTGGTTCGACGCAAAAACTCTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATATGCATACATTCTCACGCATCCTGGAACGCCCTCCATTTTCTACGACCATTTCGTTGAATGGGGATTGAAGGAGGAGCTAATCAAATTGAGCGCAATCAGGGCAAGGAATGGGATTGGGGAAAGGAGCACGGTGAATATTATGGCTTCTGACGGCGATCTATACGTTGCTGGCATTGATGGGAAAATTATAATGAAGATCGGACCGAACACCAATCTTGGAAATCTGGTTCCGTCGGATTATGAGCTTGCCGCTTCTGGAATGCAGTATGCTGTCTGGGAGAAAAAGAAATGA

Coding sequence (CDS)

ATGGGTTTCCTAGCTTCTCTGTGCTTCCTCTGCTACCTCTCTGTTTTCCTCTTCCCATCCCCCTCCTTTTCTTCTACCTTACTGTTTCAGGGCTTTAACTGGGAATCGAGTAGCAAAGGAGGATGGTACAACTCACTGAACAACATAATTCCTGATCTGGTACATGCTAAAGTTACTCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTTCTCCACAAGGATACTTACCCGGAAGGCTATATGATCTGAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTCATCGCCGCCCTCCACAATAATGGAATCAAGGCCGTAGCCGACATAGTGATCAACCACAGAACAGCAGATAAACAGGACGAGAGAGGAATTTGGTGCATTTTTGAGGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATGCAGCAGCACCTGATATCGACCATCTCAACCCACAGGTTCAGAATGAATTATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGACTTTGTAAAGGGCTATGCACCAAAAATTACAAAACTCTACATGGATCAAACCTCGCCAGATTTTGCAGTCGGTGAGAAATGGGATTCACTTTCATCTGGACCGGACGGGAAGCCCGATCCAAACCAAGACGGGCATCGCGAAGCTCTTGCAAACTGGGTTAGGGATTCAGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGTATTCTTCAAGCTGCCGTTCAGGAGGAGCTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACCTTCATCGACAACCACGACACTGGTTCGACGCAAAAACTCTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATATGCATACATTCTCACGCATCCTGGAACGCCCTCCATTTTCTACGACCATTTCGTTGAATGGGGATTGAAGGAGGAGCTAATCAAATTGAGCGCAATCAGGGCAAGGAATGGGATTGGGGAAAGGAGCACGGTGAATATTATGGCTTCTGACGGCGATCTATACGTTGCTGGCATTGATGGGAAAATTATAATGAAGATCGGACCGAACACCAATCTTGGAAATCTGGTTCCGTCGGATTATGAGCTTGCCGCTTCTGGAATGCAGTATGCTGTCTGGGAGAAAAAGAAATGA

Protein sequence

MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEKKK

Homology

[Close]

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 4.5e-196
Identity = 318/415 (76.63%), Postives = 355/415 (85.54%), Query Frame = 0


Query: 10  LCYLSVFLFPSPSFSS-TLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTHVWLPPPSQ 69
           L    +F+   P FSS  LLFQGFNWESS KGGWYNSL N IPDL +A +THVWLPPPSQ
Sbjct: 7   LSIFCLFISLLPLFSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQ 66

Query: 70  SASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIW 129
           S SP+GYLPGRLYDL+ASKYG+++ELKSLIAA H  GIK +ADIVINHRTA+++D RGI+
Sbjct: 67  SVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIY 126

Query: 130 CIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEWM 189
           CIFEGGTPDSR DWGPSFIC DDT YSDG GN DSGE Y AAPDIDHLNPQVQ ELSEWM
Sbjct: 127 CIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWM 186

Query: 190 RWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGH 249
            WLKTEIGFDGWRFDFVKGYAP I+K+YM+QT PDFAVGEKWDS+S G DGKP+ NQD H
Sbjct: 187 NWLKTEIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSH 246

Query: 250 REALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAVTFID 309
           R AL NWV  +G  +TAFDFTTKGILQAAVQ ELWRL D NGKPPG IG+KPE+AVTFID
Sbjct: 247 RGALVNWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFID 306

Query: 310 NHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGI 369
           NHDTGSTQ+LWPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKE++ KLS+IR RNGI
Sbjct: 307 NHDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGI 366

Query: 370 GERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWE 424
            E+STV IMAS+GDLYVA ID KI++KIGP  +LGNL+PS+  +A SG  YAVWE
Sbjct: 367 NEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

[Close]

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.2e-161
Identity = 274/427 (64.17%), Postives = 334/427 (78.22%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLVHAKVT 60
           M  L +L F   L   +FP+ +FSSTLLFQ FNWES  K GG+YNSL+N I D+ +A +T
Sbjct: 1   MTSLHTLLFSSLLFFIVFPTFTFSSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGIT 60

Query: 61  HVWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTA 120
           H+WLPPPSQS +P+GYLPG+LYDLN+SKYG++ ELKSLI AL+  GIKA+ADIVINHRTA
Sbjct: 61  HLWLPPPSQSVAPEGYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTA 120

Query: 121 DKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQ 180
           +++D++  +C FEGGT D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+
Sbjct: 121 ERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPR 180

Query: 181 VQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDG 240
           VQ ELSEWM WLKTEIGF GWRFD+V+GYA  ITKLY+  TSPDFAVGEKWD +  G DG
Sbjct: 181 VQKELSEWMNWLKTEIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDG 240

Query: 241 KPDPNQDGHREALANWVRDSGDTV-TAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGI 300
           K D +Q+ HR  L  W+ ++G  V TAFDFTTKGILQ+AV+ ELWRLKDS GKPPG IGI
Sbjct: 241 KLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGI 300

Query: 301 KPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIK 360
            P +AVTFIDNHDT  T   W FPSDKV+ GY YILTHPGTP IFY+H++EWGLKE + K
Sbjct: 301 MPGNAVTFIDNHDTFRT---WVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISK 360

Query: 361 LSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQ 420
           L AIR +NGIG  S+V I A++ DLY+A ID K+IMKIGP  ++G LVPS++ LA SG+ 
Sbjct: 361 LVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLD 420

Query: 421 YAVWEKK 426
           +AVWEKK
Sbjct: 421 FAVWEKK 423

[Close]

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot
Match: P27939 (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 565.8 bits (1457), Expect = 4.0e-160
Identity = 256/399 (64.16%), Postives = 308/399 (77.19%), Query Frame = 0


Query: 27  LLFQGFNWESSSK-GGWYNSLNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNA 86
           +LFQGFNWES  K GGWYN L++ + D+    VTHVWLPPPS S +PQGY+PGRLYDL+A
Sbjct: 28  VLFQGFNWESWKKQGGWYNFLHSHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLDA 87

Query: 87  SKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPS 146
           SKYGT  EL+SLIAA H+  IK VADIVINHR AD +D RGI+CIFEGGTPDSRLDWGP 
Sbjct: 88  SKYGTGAELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPD 147

Query: 147 FICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFV 206
            ICSDDT+YS+G+G+ D+G  + AAPDIDHLN +VQ ELS+W+ WLK+++GFDGWR DF 
Sbjct: 148 MICSDDTQYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDWLNWLKSDVGFDGWRLDFA 207

Query: 207 KGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTA 266
           KGY+  + K Y+D T P F V E W ++    +G+P  NQDG R+ L NW +  G   +A
Sbjct: 208 KGYSATVAKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDGDRQELVNWAQAVGGPASA 267

Query: 267 FDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDK 326
           FDFTTKG LQAAVQ ELWR+KD NGK PG IG  PE AVTFIDNHDTGSTQ  WPFPSDK
Sbjct: 268 FDFTTKGELQAAVQGELWRMKDGNGKAPGMIGWLPEKAVTFIDNHDTGSTQNSWPFPSDK 327

Query: 327 VIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYV 386
           V+QGYAYILTHPG P IFYDH  +W LK+E+  L+A+R+RNGI   S +NI+A+DGD+YV
Sbjct: 328 VMQGYAYILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNGIHPGSKLNILAADGDVYV 387

Query: 387 AGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEK 425
           A ID K+I KIG   ++GNL+PSD+ + A G  Y VWEK
Sbjct: 388 AMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCVWEK 426

[Close]

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 6.8e-160
Identity = 253/421 (60.10%), Postives = 317/421 (75.30%), Query Frame = 0


Query: 6   SLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLVHAKVTHVWLP 65
           +LC + + +V    S    + +LFQGFNWES  K GGWYN L+  + ++     THVWLP
Sbjct: 7   TLCCVVF-AVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLP 66

Query: 66  PPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDE 125
           PPS S SPQGY+PGRLYDL+ASKYGT+ ELKSLI A H+  ++ +ADIVINHR AD +D 
Sbjct: 67  PPSHSVSPQGYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDS 126

Query: 126 RGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNEL 185
           RG++C+FEGGTPD RLDWGP  ICSDDT+YS+G+G+ D+G  + AAPDIDHLNP+VQ EL
Sbjct: 127 RGVYCVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQREL 186

Query: 186 SEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPN 245
           ++W+ WL+T++GFDGWR DF KGY+  + ++Y+D T+P F VGE W SL    DGKP  N
Sbjct: 187 TDWLNWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTN 246

Query: 246 QDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAV 305
           QD  R+ L NWV   G   TAFDFTTKGILQAAVQ ELWRL D NGK PG +G  P+ AV
Sbjct: 247 QDADRQELVNWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAV 306

Query: 306 TFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRA 365
           TF+DNHDTGSTQ LWPFPSDKV+QGYAYILTHPG P IFYDH  +W L+ E+  L+ IR+
Sbjct: 307 TFVDNHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRS 366

Query: 366 RNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEK 425
           RNGI   ST++I+ ++GD+YVA IDGK+I K+GP  + G ++PSD+ + A G  Y VWEK
Sbjct: 367 RNGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 426

[Close]

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot
Match: P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 563.9 bits (1452), Expect = 1.5e-159
Identity = 258/424 (60.85%), Postives = 319/424 (75.24%), Query Frame = 0


Query: 4   LASLC-FLCYLSVFLFPSPSFSST-LLFQGFNWESSSK-GGWYNSLNNIIPDLVHAKVTH 63
           +A+LC FL    ++L P  + + T +LFQGFNW+S  K GGWYN L + + D+  A VTH
Sbjct: 5   MAALCGFLLVALLWLTPDVAHAQTQILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTH 64

Query: 64  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 123
           VWLPPP+ S SPQGY+PGRLYDLNASKYGT+ ELKSLIAA H  GIK VADIV+NHR AD
Sbjct: 65  VWLPPPTHSVSPQGYMPGRLYDLNASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCAD 124

Query: 124 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 183
            +D RG++CIF+GG P   LDWGPS IC DDT+YSDG G+ D+G  +AAAPDIDHLNP V
Sbjct: 125 DKDGRGVYCIFKGGGPRGCLDWGPSMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLV 184

Query: 184 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 243
           Q ELS+W+RWL+ ++GFDGWR DF KGY+  + + Y+    P F V E W+SLS   DGK
Sbjct: 185 QRELSDWLRWLRRDVGFDGWRLDFAKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGK 244

Query: 244 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 303
           P  NQDG R+ L NWV+  G   TAFDFTTKGILQ+AVQ ELWR++D +GK PG IG  P
Sbjct: 245 PAANQDGQRQELVNWVKQVGGPATAFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYP 304

Query: 304 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 363
           E AVTF+DNHDTGSTQ++WPFPSDKVI GYAYILTHPG P IFYDH  +W LK+E+  L+
Sbjct: 305 EKAVTFVDNHDTGSTQRMWPFPSDKVILGYAYILTHPGVPCIFYDHVFDWNLKQEINALA 364

Query: 364 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 423
           A R RNGI   S + ++A++ D+YVA +D ++I KIGP  ++GN++PSD+ + A G  Y 
Sbjct: 365 ATRKRNGINAGSKLRVLAAESDMYVAMVDERVITKIGPRIDVGNIIPSDFHIVAHGNDYC 424

Query: 424 VWEK 425
           VWEK
Sbjct: 425 VWEK 428

[Close]

BLAST of Csor.00g174210 vs. NCBI nr
Match: KAG6591375.1 (Alpha-amylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA
Sbjct: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 421 VWEKKK 426

[Close]

BLAST of Csor.00g174210 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 423/426 (99.30%), Postives = 425/426 (99.77%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQ+ELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 85  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTAD 144

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY+AAPDIDHLNPQV
Sbjct: 145 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 204

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK
Sbjct: 205 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 264

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKDSNGKPPGFIGIKP
Sbjct: 265 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 325 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 384

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA
Sbjct: 385 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 444

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 445 VWEKKK 450

[Close]

BLAST of Csor.00g174210 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 419/426 (98.36%), Postives = 422/426 (99.06%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVF+FPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 43  MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 102

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 103 VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 162

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY+AAPDIDHLNPQV
Sbjct: 163 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 222

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK
Sbjct: 223 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 282

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKDSNGKPPGFIGIKP
Sbjct: 283 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 342

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 343 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 402

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIR RNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNL+PSDY LA SGMQYA
Sbjct: 403 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYVLATSGMQYA 462

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 463 VWEKKK 468

[Close]

BLAST of Csor.00g174210 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 876 bits (2263), Expect = 0.0
Identity = 418/426 (98.12%), Postives = 421/426 (98.83%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVF+FPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY+AAPDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLS GPDGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKD NGKPPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIR RNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNL+PSDYELA SGMQYA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 421 VWEKKK 426

[Close]

BLAST of Csor.00g174210 vs. NCBI nr
Match: KAG7024254.1 (Alpha-amylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863 bits (2229), Expect = 0.0
Identity = 420/436 (96.33%), Postives = 422/436 (96.79%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASPQG--------YLPGR--LYDLNASKYGTQDELKSLIAALHNNGIKAVA 120
           VWLPPPSQSASPQG        +L  +  LYDLNASKYGTQDELKSLIAALHNNGIKAVA
Sbjct: 61  VWLPPPSQSASPQGKPSSSVAFHLKSKFVLYDLNASKYGTQDELKSLIAALHNNGIKAVA 120

Query: 121 DIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAA 180
           DIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAA
Sbjct: 121 DIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAA 180

Query: 181 PDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKW 240
           PDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKW
Sbjct: 181 PDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKW 240

Query: 241 DSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNG 300
           DSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKDSNG
Sbjct: 241 DSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNG 300

Query: 301 KPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEW 360
           KPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEW
Sbjct: 301 KPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEW 360

Query: 361 GLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDY 420
           GLKEELIKLSAIR RNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDY
Sbjct: 361 GLKEELIKLSAIRGRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDY 420

Query: 421 ELAASGMQYAVWEKKK 426
           ELAASGMQYAVWEKKK
Sbjct: 421 ELAASGMQYAVWEKKK 436

[Close]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 423/426 (99.30%), Postives = 425/426 (99.77%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQ+ELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 85  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTAD 144

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY+AAPDIDHLNPQV
Sbjct: 145 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 204

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK
Sbjct: 205 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 264

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKDSNGKPPGFIGIKP
Sbjct: 265 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 325 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 384

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA
Sbjct: 385 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 444

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 445 VWEKKK 450

[Close]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 876 bits (2263), Expect = 0.0
Identity = 418/426 (98.12%), Postives = 421/426 (98.83%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFLCYLSVF+FPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY+AAPDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLS GPDGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
           PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQ ELWRLKD NGKPPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
           AIR RNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNL+PSDYELA SGMQYA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 421 VWEKKK 426
           VWEKKK
Sbjct: 421 VWEKKK 426

[Close]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 780 bits (2013), Expect = 2.81e-283
Identity = 369/425 (86.82%), Postives = 398/425 (93.65%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60
           MGFLASLCFL YLSV LFPSPS SS LLFQGFNWESSSKGGWYNSL NIIPDLV+A++TH
Sbjct: 16  MGFLASLCFL-YLSVSLFPSPS-SSALLFQGFNWESSSKGGWYNSLKNIIPDLVNARITH 75

Query: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120
           VWLPPPSQS SPQGYLPGRLYDL+ASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA+
Sbjct: 76  VWLPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAE 135

Query: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQV 180
           KQD+RGIWCIFEGGTPD+RLDWGPSFICSDDT+YSDGKGNPDSGEPY  APDIDHL PQV
Sbjct: 136 KQDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQV 195

Query: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 240
           Q E+SEWMRWLKTEIGFDGWRFDFVKGYAP +TKLYMDQTSPDFAVGEKWDSLS GPDGK
Sbjct: 196 QKEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGK 255

Query: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKP 300
            + NQD HR++LANWV+ SGD VTAFDFTTKGILQAAVQ ELWRLKDSNGKPPGFIGIKP
Sbjct: 256 LNQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 315

Query: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360
           ESAVTF+DNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTP+IFYDHFVEWGLKEE+I+LS
Sbjct: 316 ESAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLS 375

Query: 361 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 420
            IRARN IG+ S VNI+ASD DLYVAG+DGKIIMKIG NTNLGNL+PS+++LA SGM+YA
Sbjct: 376 EIRARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYA 435

Query: 421 VWEKK 425
           VWEK+
Sbjct: 436 VWEKR 438

[Close]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 693 bits (1789), Expect = 2.11e-249
Identity = 323/418 (77.27%), Postives = 365/418 (87.32%), Query Frame = 0


Query: 9   FLCYLSVFLFPSPSFSS-TLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTHVWLPPPS 68
           FLC+L +F+    SF++ +LLFQGFNWESS KGGWYNSL N +PDL +A++THVWLPPPS
Sbjct: 6   FLCFLCLFISLCHSFAAPSLLFQGFNWESSKKGGWYNSLKNFVPDLANAEITHVWLPPPS 65

Query: 69  QSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGI 128
           QSA+P+GYLPGRLYDL+ SKYG++DELK+LIAALH  GIK VADIVINHRTA+KQD+RGI
Sbjct: 66  QSAAPEGYLPGRLYDLDVSKYGSKDELKALIAALHEKGIKVVADIVINHRTAEKQDDRGI 125

Query: 129 WCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEW 188
           W IFEGGTPD+RLDWGPSFIC DD  YSDG GNPDSGE Y  APDIDH NPQVQ ELSEW
Sbjct: 126 WSIFEGGTPDARLDWGPSFICKDDIAYSDGTGNPDSGEGYDPAPDIDHRNPQVQKELSEW 185

Query: 189 MRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDG 248
           M WLKTEIG+DGWRFDFVKGYAP I K YM+QT PDFAVGE W+SL+ G DGKP+PNQD 
Sbjct: 186 MNWLKTEIGYDGWRFDFVKGYAPSIMKNYMEQTKPDFAVGENWNSLAYGDDGKPNPNQDA 245

Query: 249 HREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAVTFI 308
           HR ALA WV  +G  VTAFDFTTKGILQAAV+ ELWRLKDSNGKPPG IGIKPESAVTFI
Sbjct: 246 HRGALAEWVGSAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPESAVTFI 305

Query: 309 DNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNG 368
           DNHDTGSTQ +WPFPSDKV+QGYAYILTHPGTP+IFYDHF +WGLKEE+ KL+AIR+RNG
Sbjct: 306 DNHDTGSTQNIWPFPSDKVMQGYAYILTHPGTPTIFYDHFFDWGLKEEISKLTAIRSRNG 365

Query: 369 IGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEKK 425
           I  RS V+I+ASDGDLYVAGID KIIMKIGP  +LGNL+PS+++++ SG  YAVWEKK
Sbjct: 366 INTRSIVSILASDGDLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSTSGKDYAVWEKK 423

[Close]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL
Match: A0A2N9FNN7 (Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1)

HSP 1 Score: 691 bits (1784), Expect = 1.22e-248
Identity = 327/426 (76.76%), Postives = 367/426 (86.15%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFS-STLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVT 60
           M  L SLCFLC L + L  SPSF+   +LFQGFNWESS+KGGWYN L N IPDL +A +T
Sbjct: 1   MNILTSLCFLC-LCISL--SPSFAVPAILFQGFNWESSNKGGWYNLLKNSIPDLANAGIT 60

Query: 61  HVWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTA 120
           HVWLPPPSQS +PQGY+PGRLYDL+ASKYG++DELKSLIAALH  GIKA+ADIVINHRTA
Sbjct: 61  HVWLPPPSQSVAPQGYMPGRLYDLDASKYGSKDELKSLIAALHEKGIKALADIVINHRTA 120

Query: 121 DKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQ 180
           +KQD RGIWCIFEGGTPD RLDWGPSFIC DDT YSDG GNPDSG+ +  APDIDH+NPQ
Sbjct: 121 EKQDGRGIWCIFEGGTPDGRLDWGPSFICKDDTAYSDGTGNPDSGKDFGGAPDIDHVNPQ 180

Query: 181 VQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDG 240
           VQ ELSEWM WLKTEIGFDGWRFDFV GYA   TK+YM+QT PDFAVGE W+SLS G  G
Sbjct: 181 VQKELSEWMNWLKTEIGFDGWRFDFVLGYASSFTKVYMEQTKPDFAVGENWNSLSYGDGG 240

Query: 241 KPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGIK 300
           KP+PNQD HR AL +W++ +G  VTAFDFTTKGILQAAV+ ELWRLKDSNGKPPG IGIK
Sbjct: 241 KPNPNQDAHRNALVDWIQAAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIK 300

Query: 301 PESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKL 360
           PE+AVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDH  +WGLK+E+ KL
Sbjct: 301 PENAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHLFDWGLKDEISKL 360

Query: 361 SAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQY 420
           +AIR+RNGI  +  VNI+ASD DLYVAGID KIIMKIGP  +LGNL+PS+++++ASG  Y
Sbjct: 361 TAIRSRNGINTQGIVNILASDADLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSASGKDY 420

Query: 421 AVWEKK 425
           AVWEKK
Sbjct: 421 AVWEKK 423

[Close]

BLAST of Csor.00g174210 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 570.9 bits (1470), Expect = 8.8e-163
Identity = 274/427 (64.17%), Postives = 334/427 (78.22%), Query Frame = 0


Query: 1   MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLVHAKVT 60
           M  L +L F   L   +FP+ +FSSTLLFQ FNWES  K GG+YNSL+N I D+ +A +T
Sbjct: 1   MTSLHTLLFSSLLFFIVFPTFTFSSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGIT 60

Query: 61  HVWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTA 120
           H+WLPPPSQS +P+GYLPG+LYDLN+SKYG++ ELKSLI AL+  GIKA+ADIVINHRTA
Sbjct: 61  HLWLPPPSQSVAPEGYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTA 120

Query: 121 DKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQ 180
           +++D++  +C FEGGT D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+
Sbjct: 121 ERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPR 180

Query: 181 VQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDG 240
           VQ ELSEWM WLKTEIGF GWRFD+V+GYA  ITKLY+  TSPDFAVGEKWD +  G DG
Sbjct: 181 VQKELSEWMNWLKTEIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDG 240

Query: 241 KPDPNQDGHREALANWVRDSGDTV-TAFDFTTKGILQAAVQEELWRLKDSNGKPPGFIGI 300
           K D +Q+ HR  L  W+ ++G  V TAFDFTTKGILQ+AV+ ELWRLKDS GKPPG IGI
Sbjct: 241 KLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGI 300

Query: 301 KPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIK 360
            P +AVTFIDNHDT  T   W FPSDKV+ GY YILTHPGTP IFY+H++EWGLKE + K
Sbjct: 301 MPGNAVTFIDNHDTFRT---WVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISK 360

Query: 361 LSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQ 420
           L AIR +NGIG  S+V I A++ DLY+A ID K+IMKIGP  ++G LVPS++ LA SG+ 
Sbjct: 361 LVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLD 420

Query: 421 YAVWEKK 426
           +AVWEKK
Sbjct: 421 FAVWEKK 423

[Close]

BLAST of Csor.00g174210 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 377.9 bits (969), Expect = 1.1e-104
Identity = 187/398 (46.98%), Postives = 247/398 (62.06%), Query Frame = 0


Query: 27  LLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNAS 86
           +L QGFNWES+  G WY  L     +L     T +WLPPP++S SP+GY+P  LY+LN S
Sbjct: 497 ILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 556

Query: 87  KYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSF 146
           +YGT DELK  +   H  GIK + D V+NHR A  +++ G+W +F G     RL+W    
Sbjct: 557 RYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNWDDRA 616

Query: 147 ICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVK 206
           + +DD  +  G+GN  SG+ + AAP+IDH    V+ ++ EW+ W+  E+G+DGWR DFV+
Sbjct: 617 VVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVR 676

Query: 207 GYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAF 266
           G+     K YMD + P FAVGE WDSLS    G+ D NQD HR+ + +W+  +     AF
Sbjct: 677 GFWGGYVKDYMDASKPYFAVGEYWDSLSY-TYGEMDYNQDAHRQRIVDWINATSGAAGAF 736

Query: 267 DFTTKGILQAAVQE-ELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDK 326
           D TTKGIL  A+Q+ E WRL D  GKPPG +G  P  AVTFI+NHDTGSTQ  W FP  K
Sbjct: 737 DVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGK 796

Query: 327 VIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYV 386
            +QGYAYILTHPGTP++F+DH        E+  L ++R R  +  RS VNI  S+ D+Y 
Sbjct: 797 EMQGYAYILTHPGTPAVFFDHIFS-DYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYA 856

Query: 387 AGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWE 424
           A ID K+ MKIGP          ++ +A  G  Y VWE
Sbjct: 857 AIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Csor.00g174210 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 363.2 bits (931), Expect = 2.8e-100
Identity = 180/402 (44.78%), Postives = 251/402 (62.44%), Query Frame = 0


Query: 27  LLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTHVWLPPPSQSASPQGYLPGRLYDLNAS 86
           ++ Q +NWE S K  W+ +L+  +PD+  +  T  WLPPPSQS +P+GYLP  LY LN S
Sbjct: 27  VILQAYNWE-SHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-S 86

Query: 87  KYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSF 146
            YG++  LKSL+  +    ++A+ADIVINHR    +   G++  ++G      L W    
Sbjct: 87  AYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHA 146

Query: 147 ICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVK 206
           +    T  + G GN  +G+ +   P++DH    V+ ++  W+RWL+  +GF  +RFDF +
Sbjct: 147 V----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFAR 206

Query: 207 GYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAF 266
           GY+    K Y+    P F+VGE WDS +    G  D NQD HR+ + +W+  +G    AF
Sbjct: 207 GYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRIISWIDATGQISAAF 266

Query: 267 DFTTKGILQAAVQEELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKV 326
           DFTTKGILQ AV+ + WRL D+ GKPPG +G  P  AVTF+DNHDTGSTQ  WPFPS  V
Sbjct: 267 DFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHV 326

Query: 327 IQGYAYILTHPGTPSIFYDHFVEWG--LKEELIKLSAIRARNGIGERSTVNIMASDGDLY 386
           ++GYAYILTHPG P +FYDHF +WG  + ++++KL  IR R  I  RSTV ++ ++ +LY
Sbjct: 327 MEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLY 386

Query: 387 VAGIDGKIIMKIGPNTNLGNLVPS--DYELAASGMQYAVWEK 425
            A +  KI MK+G     G+  PS  D+ LA SG +YAVW K
Sbjct: 387 AAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

The following BLAST results are available for this feature:

BLAST of Csor.00g174210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	4.5e-196	76.63	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
Q8VZ56	1.2e-161	64.17	Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1	[more]
P27939	4.0e-160	64.16	Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2...	[more]
P27934	6.8e-160	60.10	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]
P27932	1.5e-159	60.85	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2...	[more]

BLAST of Csor.00g174210 vs. NCBI nr


Match Name	Identity	Description
KAG6591375.1	100.00	Alpha-amylase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022936258.1	99.30	alpha-amylase, partial [Cucurbita moschata]	[more]
XP_023536126.1	98.36	alpha-amylase, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022976296.1	98.12	alpha-amylase [Cucurbita maxima]	[more]
KAG7024254.1	96.33	Alpha-amylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Csor.00g174210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FCS4	0.0	99.30	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6J1IGI6	0.0	98.12	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A6J1CMA4	2.81e-283	86.82	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A2I4EXA7	2.11e-249	77.27	Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1	[more]
A0A2N9FNN7	1.22e-248	76.76	Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1	[more]

BLAST of Csor.00g174210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	8.8e-163	64.17	alpha-amylase-like	[more]
AT1G69830.1	1.1e-104	46.98	alpha-amylase-like 3	[more]
AT1G76130.1	2.8e-100	44.78	alpha-amylase-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 105..116 score: 49.02 coord: 301..313 score: 51.43 coord: 197..208 score: 40.85
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 26..363 e-value: 3.6E-52 score: 189.3
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 58..209 e-value: 1.1E-13 score: 51.5
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 364..424 e-value: 1.0E-31 score: 121.3
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 365..423 e-value: 3.3E-18 score: 65.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 26..367 e-value: 5.4E-142 score: 475.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..248
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 3..425
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 3..425
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 28..374 e-value: 3.70254E-162 score: 456.682
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 368..424
IPR013775	Alpha-amylase, plant	PIRSF	PIRSF001028	Alpha-amylase_plant	coord: 1..425 e-value: 1.6E-217 score: 720.4
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 368..425 e-value: 2.3E-23 score: 84.0
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 27..352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g174210.m01	Csor.00g174210.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding