Csor.00g163300 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g163300
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	tRNA (guanine(9)-N(1))-methyltransferase
Location	Csor_Chr13: 6619359 .. 6624611 (+)
RNA-Seq Expression	Csor.00g163300
Synteny	Csor.00g163300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAGCGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGGTTTGTTCTCTTGTTACTCACTTCATTTGGTTGATTAACTATTATTTGTGGTGGTGCGAATACTGGATTGTTTTCTTATATTTTAGAGGTTTGGTAAATGTTTCTAGTAGATGGAATGTAGAGCATCTTAGAAATGAAATTGATTGTTTCTTAGAAGACCATACATTTGAGATGAAGGGCTTCTTCTCTGAGGTCCCTTAAGAACTGAAATGGAATGTATTCTAGGAATACCATAAATTTTGGAGGTTTTCTAAGCCAAATAGTTTGAGAAGGAAAGCCTTGGCAGACTCAAATCTAGTTTTAGTAGTTGATCAAGTGACGATTGATTGTTTCTTGGAAGACAATCAAATGCCGCCTGAGTCAACACAAACGCATGACTTGAAGTGCCTCTCTACCTTTTCTTTATTTTTCTTTCCCACTTTTGTAGAACAATTGATCATCTTTGGTTTCTTGCAAGTAGCAAAAGATCCTTTCAGTATCCAAAAAAGATGCAGCTACATTGGATTCTCCCTAAATCTCTTCTGTTGCAGTTAAGTGCATCAAACTACCCACCATTTGGTTATAAAGTGTGTCGTTCACCTTCTTTCTTCTTTCCTTCACGGTCTTGATTTAGTTCCAAGCCAGACTCAGTTCGAGTATGCATAGGATTGTACTTTGTTTGAAAAGCATTTAAAATTTCTTGCAACAAACCTTTGAAACTGTAAAGATTTTTTTGTACTATTTGGGAGGAAAAGAAAAAGCCTGTGGCTGTTTGTAATATTTCAATACCAAGGAAATAATACATACTTCAAGATCACATCATTTCTAGTATTAGTAAGATCATCTACGTACTAACAAACAATGAGTTCATTGCCTCCATCTCCAGGTTTTGTGGAAAGGTTATGCTCATAATGATATTTCCTGAAAGCTTTCTTTAAGAAATAAATACCAACAATACTACACGAAAGCTCTTGTAGCATGTTTTAGTCTGTTACTTCTTTGAAACTTATACGCATTAGCTCATCTCCAATCTTTTAACGTGGTGATGATTTCAGTTGGTTAATTTCTTTTATCCTTAAATATGCATTCCACCTAGGGAAGCTCCCGCAAAATTTCCTTAGTGTTGGATAAATTTCCCACACATTTTGACAATGAATGAAAGGTGTCTATGTAACATCTATCATGTGGGATATTGCATATTTTGATTTGAAAAATACTTGGCTTGCCTACACACCAGCTGCTGACGGTAGGATACCCTACTGCCTTTCTATTATGGTGTATTTTTAGTGGCTCAAAAAAGTTTAGTGGAGTCTACTTATCTATTAAATGCAATAGAAAGAGACAATAAAAATGATCTATATTAGAAATTAGTTGGATATTTTAATGACTAGTATGTAGACAGTATTGTTCTTTCAATCCATCTCCCACAAGGAGGAGTAGTTTGAGCTTCTACCATGGGATCCTTATCCTTCATTTCCAACATTAGAATTTATATATATATACAAACAACAATTTCTTCATGTCATTGATAAGACGTAATTACAAAAGAAGGCAAACTATGTGCCGAAGAGGGTATAATTTACACCAAGAGAAAGCAATAGGTATAATTTACACTAGTACAACTTCTTAACCTTGTTTCACCTTTGTTTTCTTCAAGCCTTACAAATGATGAAAATGAGGTTTGAGTCTCTTTTCCCCAAAACTGAATTCTCCAAAAAAAATTAATGAATTTTATGAGTGAATATTACTCATCCTTGATATATTTTTTTAAACAAAGTCTGCTCTCGCTCCAAGACCCAGAATTTGCTCACATAAAGAACTTTCCTTTGTAAGCTCAACTGTAGATTTGAAAACGTGATCTTTGGGTAGGTGTTATACATCCTTGATGTCGACTTATCCCCTGGTCTAGTAATGTCACATTTTTCCAGCTGAAGGAGATTCACGTGTGAGATTTTTGATATGTACCCCTCTATTGGTATTGTGATTTTCCTTCACTTCCTCTAATTGAACCAAATGACCTGGTTTGTAGAACTTGCTGTCTACACTGCTTTCCTTGGGTAACCCTTTATGATATAATGGAAGGGCTCCCAACCTTCCCTCCCCCTCCTACAAGAATCAAGAGTGTATTTTTCCATTGCCATCATTTTTTAAGAATTCATGCTGAAGCATCCTGTCATTCATGCTGAAGCATCCTGTCATTCATGCTGAAGCATCCTGTCAGATATATTCTCCACTCAACAAAATAATCATTACTCGAAATTCTTAGAATAATTTGGAATTAGATTGTACTGAATCTATAGCGGTAGCTTTGGAACAATAACTCAACAACTATATGGATACCTGGTTGATAGAGAGTGACAAGAACCATTGTTTTCTCATTCAAGCCCTGGATAGCTCTCTCCGTACCTACTGGCCACCACGATCTTAAATCAATAAAATGCTTGACTAATCCCACCCTCTCGAAACTCATAACCATAGAGAACCATTGGAAAGGGGGGAAAAGAGAATGAAGAAGATGAAGAAGATGAAGAAGAACAGGGGAATGCATCATAGGAGTCGCTCTTTTCAAAATTTAACTTTCCACATGTTTAATAAAATCATTAGTTTGTAGGTTTAAATTTAGCTTTGAATCATATGATTATACAAGATTCCTATTGGGAGACAATGACTTTATATTGCACCTAAATACTCGGTACTTCTACTAATGTCTGGGATGAGCTCTATTTAATTAGATTAGACTAAGATGCCACATTTTTTGATACTCTTATTGACTCATATATAATTCAAGATTCAGAAATAGAAACTGTCAAAGCATGACACTACTTAGTAGACTTTTCCTACGTTATTTGTCATGCTAAGACATTTGTGAAACACTTCATCATCCCAAAGCTGGTTTATTGTCTTCACATTAAGTTATGGTTAGGATCCTGCCAAATAGTATCTTTTTTTCAATTTACAAATGATTCACACTTCTGACCAATTACTGAGCATGTTTTATCCTATGCAGTCGTCTTTATTTTGATTTTTAATGCACGGTTAATTTTTCAAGAATGATTCTATAATCTCCTGGAAACTGCAAGGTTTTTGAGTATCTTTATTGGGTAAAAACCTGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTATACTTCGGTCTTCACATGCATATCATTGTTATCCGCATTTCATAGACTAAATTCTTTCATGCTAGACTTATAAAATGGCAGATTTAGAATACATGTGTGCTCCCTACTTTCTTCTAATTTTTATATTTTTATTATGAGAAATTGAGCTTCTATTGAGAAAAATGAAATAATATAATGGGTATATAAGAAAAAGAATATACTCATGTCAGACATGCGTTCCGAACGTGTTCGACATTGCTTCTCCTCCTATATTAGTGTAGCCTGCTTCATATACTCCAACCAAATAAAACAAGACCTAGCTGATCATTACAAAAAACCCTAACGATGGAAGCCCACAAAGAGACATTGAATCTAGCAGCATTCCAGATCTCTTCCCAAGATCTTTTAACATCTTTGAAACTTTTGCCATTCCTCTTTGACCAAATACCCCATGAAATAGGAAAAAAATAAAACTAGCAAGTCAAAGGACATCATAAAACAACAATCATGGGTACGTGCCAATTATATAGTAAATGACTCCAAAAACAAATCCAAATAGACTAAGCAAATCGACTATTTCAAAGAACATGGTTGTGATTCTCGGACGCTTTACACAAGATGCAAAGATGGTAAAGATGTGGGAACTTGACGCAAAGAGTCTTATCTCTCGTGATCCCAACCAACAATTAATGAGAGTACTACCAAGGGATGTATGGGGAATATTAGTGGGATGGTATTTTAGTGGTCTCTTAGTTATATAAATATGGGTAGTTGGGCATGTTACAGAGGGCAATGATTTTAGGTATTGTCCTGTGTGTGACTTTGGGAGAGACATATTACACCGTGGACACAGTGTAATAGCCCAAGTCCATCGCTAGCAGATATTGTCATTTTTTTTATTTTTCCTTGTAGGCTCCCCGTCAAAGTTTTAAAACGCATTCGCTAGGGAGAGGTTTATCCACCTTTATAAGAAATGCTCCATTCCCCTCTCCAATGGAATGAAAAACAAATGGGAGATGAGGCATCAACCTGTCACAATGATGAAGAAGTCCTAACCAAATCTATAAGCATAGGTTTTTGAGATTACCTACTTACAAAATCACGAACTGACCTCGTATAATTTAGTAATTGTTCTCTTACAATATGTTGGACGTTTTCAATTTCATTTCCAGGTCCTTACTGTGAATCAAGTCCTAGAGATTCTACTCAAGTACATAGAAACGAGAGATTGGAAGGCTTCTTTCTTCCAAGTCGTTCCTCAAAGAAAAAGATGTGAAGCTGAATGTAATAGTGACCATGCAGTTGATGGGGAAGAAAATGGAGATAGAGATGATGAGCAAGAGACTAAGAAGACCAAGTGCGATGAAGTATCTTCCAGTAACCATACTACTTTATAA

mRNA sequence

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAGCGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTCCTTACTGTGAATCAAGTCCTAGAGATTCTACTCAAGTACATAGAAACGAGAGATTGGAAGGCTTCTTTCTTCCAAGTCGTTCCTCAAAGAAAAAGATGTGAAGCTGAATGTAATAGTGACCATGCAGTTGATGGGGAAGAAAATGGAGATAGAGATGATGAGCAAGAGACTAAGAAGACCAAGTGCGATGAAGTATCTTCCAGTAACCATACTACTTTATAA

Coding sequence (CDS)

ATGGAGGCCAGAGAAGAGGACCAGAGCGCCAAAATGGATCCTCCTTGTTCCCCTTCCGGCTGCAATCCTCCAGAGCCCCCCCTCTCCAAGAATGCAAGGAAGAAGCTACTGAAACAACAGCGTTACGAAGCGAAGAAAGCGGAGAAGAAAGCTCAGGAAAAAGAGCAGAAGAAGAAAGATATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTACGGAGGAGGAGAGAGCCAAGCTGATCGAGTCTAGGAAAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAAGGAGAAGAAGATTGAGCGGCTCAACAGGGCTAAAGATTGTGGTCAGAAGATTGTCATCGACCTTGAATTTTCTGACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGATAATGTATTGTTATGCAGTGAATGGAAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTGCAAAGGCTTCCAGGATTTGACAAATGGATCATTGAGAGGGAACATAGATCATACATAGATGCTTTGCAGGACCAGAAGGAAAATTTGGTCTATCTCACAGCTGATTCAGAAACTGTGCTTGATGATCTTGATCTAAAGAAAATATATATCATTGGCGGGTTAGTTGATCGGAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCAATAGGAAGTTACTTGAAGATGTCTAGCTCTCAGGTCCTTACTGTGAATCAAGTCCTAGAGATTCTACTCAAGTACATAGAAACGAGAGATTGGAAGGCTTCTTTCTTCCAAGTCGTTCCTCAAAGAAAAAGATGTGAAGCTGAATGTAATAGTGACCATGCAGTTGATGGGGAAGAAAATGGAGATAGAGATGATGAGCAAGAGACTAAGAAGACCAAGTGCGATGAAGTATCTTCCAGTAACCATACTACTTTATAA

Protein sequence

MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNSDHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL

Homology

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BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-36
Identity = 106/301 (35.22%), Postives = 175/301 (58.14%), Query Frame = 0


Query: 25  EPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLI 84
           +P LSK+A K+L +QQ ++A + EK+A+ + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYC 144
           E+ + ++           +KK  RL +      +IV+D  F DLM   EI+SL QQ+  C
Sbjct: 89  EAGEVVK----------SQKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEREH---RSYIDALQDQKENLVY 204
           ++ N     P  L+ T   G ++T+   +    +   +R +   +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 264
           L+ADS+  + +LD  KIYIIG +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 265 LTVNQVLEILLKYIETRDWKASFFQVVPQRK--RCEAECNSDHAVDGEENGDRDDEQETK 321
           LTVNQV EIL  ++E RDW+ +F +V+P+RK    +++ + D + D     ++ D +  K
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRKGILLKSDESFDVSEDTRSQSNQSDSELEK 303

[Close]

BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 4.1e-35
Identity = 108/310 (34.84%), Postives = 166/310 (53.55%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++ ++K + KE++K                            
Sbjct: 39  PISKRQLKKLMKQKQWEEQREQRKEKRKEKRK---------------------------- 98

Query: 87  RKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYA 146
           RK L + ++   S+  ++K  R + A+    ++VID  F DLM   +I  L +QI  CYA
Sbjct: 99  RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEREHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+LVYL
Sbjct: 159 ENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEHYSEL----IKKEDLVYL 218

Query: 207 TADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 266
           T+DS  VL DLD  K Y+IGGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +VL
Sbjct: 219 TSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVL 278

Query: 267 TVNQVLEILLKYIETRDWKASFFQVVPQR-------KRCEAECNS--------DHAVDGE 318
            VN V EI+L+++ETRDW+ +FF ++PQR       K CE+            D A +GE
Sbjct: 279 AVNHVFEIILEFLETRDWQEAFFTILPQRKGAVPAHKACESSPQDHQSLPEGWDSASEGE 315

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BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 118/349 (33.81%), Postives = 184/349 (52.72%), Query Frame = 0


Query: 2   EAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDI 61
           E +EE Q  ++   C          P+SK   KKL+KQ+++E ++  +K + KE      
Sbjct: 23  EDQEESQKPRLGEGCE---------PISKRQMKKLIKQKQWEEQRELRKQKRKE------ 82

Query: 62  ERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVI 121
           +RKRK+ E                     R+ +M   S+  ++K  R +       +++I
Sbjct: 83  KRKRKKLE---------------------RQCQMEPNSDGHDRKRVRRDVVHST-LRLII 142

Query: 122 DLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW- 181
           D  F  LM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  W 
Sbjct: 143 DCSFDHLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWK 202

Query: 182 --IIEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKK 241
              I+ EH S +     +KE+L+YLT+DS  +L +LD  K Y+IGGLVD N  KG+T K+
Sbjct: 203 DIHIKPEHYSEL----IKKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQ 262

Query: 242 AQEQGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQR------ 301
           A + GI  A+LP+G+++KM+S +VL VN V EI+L+Y+ETRDW+ +FF ++PQR      
Sbjct: 263 ASDYGINHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPT 322

Query: 302 -KRCEAECNSDHAVDGEENGDRDD--EQETKKTKCD-----EVSSSNHT 333
            K CE+  + + +V  EE G   D  E+E  + + D     +    NHT
Sbjct: 323 DKACESASHDNQSVRMEEGGSDSDSSEEEYSRNELDSPHEEKQDKENHT 330

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BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 129/357 (36.13%), Postives = 192/357 (53.78%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQE---KEQK 60
           +EA  E     +DP       N  +   S++A      Q+   A ++E KAQ+   K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNR------ 120
           KK +  K+K+W        E++R    + RK  R++R  +R  + E  I+  +R      
Sbjct: 63  KKFL--KQKQW--------EDQRELRKQKRKEKRQKRKLERQAQAEHNIDANSRKRFRHE 122

Query: 121 AKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQ 180
            +    +++ID  F DLM   ++  L +QI  CYA N R   P  L+LT   G++++ + 
Sbjct: 123 VQPSALRLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMD 182

Query: 181 RLPGFDK-WI------IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGL 240
               +DK WI      I+ EH  Y D +  +KE+LVYLT+DS  VL +LD  K YIIGGL
Sbjct: 183 E---YDKGWINWKDIHIKPEH--YKDLI--KKEDLVYLTSDSPEVLSELDETKAYIIGGL 242

Query: 241 VDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASF 300
           VD N  KGIT KKA E GI  A+LP+G+++KM++ +VL VN V EI+L ++E ++WK +F
Sbjct: 243 VDHNHHKGITYKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAF 302

Query: 301 FQVVPQRKRC--------EAECNSDHAVDGEENGDRD---DEQET--KKTKCDEVSS 329
           F V+PQRK          + EC +    DGE++ D D   DE  T     +C+E +S
Sbjct: 303 FSVLPQRKGAIPLTETGEQPECRASEQEDGEDS-DSDSSIDESATIQPSVRCEEQNS 334

[Close]

BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-34
Identity = 112/324 (34.57%), Postives = 173/324 (53.40%), Query Frame = 0


Query: 27  PLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDIERKRKEWEETLASVTEEERAKLIES 86
           P+SK   KKL+KQ+++E ++  +K + KE      +RKRK+ E      +  + +     
Sbjct: 39  PISKRQMKKLMKQKQWEEQRELRKQKRKE------KRKRKQLERQCQPESNSDGS----D 98

Query: 87  RKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVIDLEFSDLMTPSEIHSLVQQIMYCYA 146
           RK +R++                        +++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RKRIRRD------------------VVHSPLRLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEREHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S       QKE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEF----IQKEDLIYL 218

Query: 207 TADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 266
           T+DS  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVL 278

Query: 267 TVNQVLEILLKYIETRDWKASFFQVVPQRK-------RCEAECNSDH---AVDGEENGDR 326
            VN V EI+L+Y+ETRDW+ +FF ++PQRK        CE+ C+ D     V+G  N D 
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACES-CSHDKKFARVEGGLNSDS 329

Query: 327 DDEQ---ETKKTKCDEVSSSNHTT 334
            +E+   E   T  +E     ++T
Sbjct: 339 SEEENRHELDSTHEEEKQDKENST 329

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BLAST of Csor.00g163300 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 640 bits (1651), Expect = 4.26e-231
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633 bits (1633), Expect = 2.36e-228
Identity = 331/334 (99.10%), Postives = 332/334 (99.40%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. NCBI nr
Match: XP_022927615.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata])

HSP 1 Score: 632 bits (1631), Expect = 4.77e-228
Identity = 329/334 (98.50%), Postives = 332/334 (99.40%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQD KENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILL+YIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDG+ENGDRDDEQETKKTKCDEVSSSNHTTL
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. NCBI nr
Match: XP_022973151.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 627 bits (1616), Expect = 9.21e-226
Identity = 326/334 (97.60%), Postives = 331/334 (99.10%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWE+TLASVTEEERAKLIESR+SLRKERMGKRSEEKEKKIERLNRAKDCGQKI+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDGEENGDRDDEQE+KK KC EVSSSNHTTL
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. NCBI nr
Match: XP_038893739.1 (tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida])

HSP 1 Score: 566 bits (1459), Expect = 8.59e-202
Identity = 302/336 (89.88%), Postives = 313/336 (93.15%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPP SP   NPPEPPLSKNA+KKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAVEEDQSAKMDPPPSPFAFNPPEPPLSKNAKKKLLKQQRYEAKKAEKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWE+TLASVTEEER+KLIESR+SLRKERMGKRSEE+EKKIERLNRAKDCGQ IV
Sbjct: 61  IERKRKEWEDTLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNRAKDCGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTAD+ETVLDDLD KKIYIIGGLVDRNRWKGITMKKA E
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGITMKKAHE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKRC A+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCGADSNS 300

Query: 301 DHAVDGEENGDRD----DEQETKKTKCDEVSSSNHT 332
           DH VD EENGD D    DE E KKTKCDEVSSS+ T
Sbjct: 301 DHPVDEEENGDGDGDGDDEHEMKKTKCDEVSSSDDT 336

[Close]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 632 bits (1631), Expect = 2.31e-228
Identity = 329/334 (98.50%), Postives = 332/334 (99.40%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQD KENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILL+YIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDG+ENGDRDDEQETKKTKCDEVSSSNHTTL
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 627 bits (1616), Expect = 4.46e-226
Identity = 326/334 (97.60%), Postives = 331/334 (99.10%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEA EEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWE+TLASVTEEERAKLIESR+SLRKERMGKRSEEKEKKIERLNRAKDCGQKI+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNHTTL 334
           DHAVDGEENGDRDDEQE+KK KC EVSSSNHTTL
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSNHTTL 334

[Close]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 1.04e-194
Identity = 288/331 (87.01%), Postives = 307/331 (92.75%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           MEA EE  + KMDPPCS S CNPPEPPLSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LASVTEEERAKLIESR++LRKERMGKRSE++EKKIERL+ A+D GQ IV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTP EIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFF+VVPQRKRC  + NS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSNH 331
           DH VDGEENGD D++ ETKKTKCDEVSSS++
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDN 331

[Close]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 2.68e-194
Identity = 286/327 (87.46%), Postives = 303/327 (92.66%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           ME  E   S K+DPPCSPS CNPPEP LSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LA VTEEERAKLIESR++LRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRC A+ NS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVS 327
           DH VDGEENGD D++QETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

[Close]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 2.68e-194
Identity = 286/327 (87.46%), Postives = 303/327 (92.66%), Query Frame = 0


Query: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60
           ME  E   S K+DPPCSPS CNPPEP LSKNARKKLLKQQRYEAKKA+KK Q KEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120
           IERKRKEWEE LA VTEEERAKLIESR++LRKERMGKRSE++EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+E RSYIDALQ+QKENLVYLTADSETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRC A+ NS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHAVDGEENGDRDDEQETKKTKCDEVS 327
           DH VDGEENGD D++QETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

[Close]

BLAST of Csor.00g163300 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 399.8 bits (1026), Expect = 2.1e-111
Identity = 229/336 (68.15%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 3   AREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKDIE 62
           A   D +   D P S        PPLSKNA+KK LKQQRYEAKKAEKKAQEKE K+K+ E
Sbjct: 4   ATGNDDNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGE 63

Query: 63  RKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIVID 122
           RK KEWEETLA+ TEEER KLIESRKSLRKERM KRSEEKEKKIERLN+AK+ GQKIV+D
Sbjct: 64  RKLKEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVD 123

Query: 123 LEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIE 182
           ++F+ LM+ SEI SLVQQIMYCYAVNGR  SP HLWLTG  G+M T+L +LPGF+KW IE
Sbjct: 124 VDFAHLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIE 183

Query: 183 REHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQG 242
           +E R YI+A+ DQK+NLVYLTADSETVLDDLD K IYIIGGLVDRNR+KGITM KAQEQG
Sbjct: 184 KESRCYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQG 243

Query: 243 IQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKR-------CE 302
           I+TAKLPIG Y+KMSSSQVLTVNQVLEIL+K++ETRDWK +FF V+PQRKR       C 
Sbjct: 244 IKTAKLPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLDPVDCS 303

Query: 303 -----AECNSDHAVDGEENGDRDDEQETKKTKCDEV 327
                +E + +   +  E+ D+ D  E KK  C EV
Sbjct: 304 KLEHISEEHQEKDTEANESADKHDLLERKKV-CVEV 338

The following BLAST results are available for this feature:

BLAST of Csor.00g163300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O14214	1.7e-36	35.22	tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 ...	[more]
Q8C1Z8	4.1e-35	34.84	tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=...	[more]
Q8TBZ6	2.0e-34	33.81	tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1	[more]
Q66JJ4	2.0e-34	36.13	tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=...	[more]
Q3MHI8	4.5e-34	34.57	tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1	[more]

BLAST of Csor.00g163300 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583717.1	4.26e-231	100.00	hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023520714.1	2.36e-228	99.10	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_022927615.1	4.77e-228	98.50	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata]	[more]
XP_022973151.1	9.21e-226	97.60	tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima]	[more]
XP_038893739.1	8.59e-202	89.88	tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida]	[more]

BLAST of Csor.00g163300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EPG7	2.31e-228	98.50	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1I7W5	4.46e-226	97.60	tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A0A0LYJ3	1.04e-194	87.01	SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3...	[more]
A0A5D3CW30	2.68e-194	87.46	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CL37	2.68e-194	87.46	tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of Csor.00g163300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47680.1	2.1e-111	68.15	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 37..112
None	No IPR available	PIRSR	PIRSR016323-1	PIRSR016323-1	coord: 37..317 e-value: 5.5E-84 score: 279.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 298..334
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 298..326
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..71
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..71
None	No IPR available	PANTHER	PTHR13563:SF17	BNAA06G35330D PROTEIN	coord: 8..317
None	No IPR available	CDD	cd18089	SPOUT_Trm10-like	coord: 117..287 e-value: 1.13243E-86 score: 256.308
IPR016009	tRNA methyltransferase TRMD/TRM10-type domain	PFAM	PF01746	tRNA_m1G_MT	coord: 127..292 e-value: 1.9E-29 score: 102.7
IPR038459	tRNA methyltransferase TRM10-type domain superfamily	GENE3D	3.40.1280.30		coord: 97..292 e-value: 6.6E-70 score: 236.7
IPR016653	tRNA (guanine(9)-N(1))-methyltransferase TRM10/TRM10A	PIRSF	PIRSF016323	tRNA_m1G_Mtase_euk	coord: 1..331 e-value: 8.3E-89 score: 295.6
IPR007356	tRNA (guanine-N1-)-methyltransferase, eukaryotic	PANTHER	PTHR13563	TRNA (GUANINE-9-) METHYLTRANSFERASE	coord: 8..317
IPR028564	tRNA methyltransferase TRM10-type domain	PROSITE	PS51675	SAM_MT_TRM10	coord: 105..295 score: 47.926914

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g163300.m01	Csor.00g163300.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002939	tRNA N1-guanine methylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity