Csor.00g157350 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g157350
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionL-galactose dehydrogenase
LocationCsor_Chr19: 7971252 .. 7974781 (-)
RNA-Seq ExpressionCsor.00g157350
SyntenyCsor.00g157350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCTTCTTATCCCAAGCTCCAGCTTCGGAAGCTGGGGAACACCGGCCTCAAAACCAGCAGTGTCGGCTTTGGTGCCTCGCCTCTTGGCAGCGTCTTTGGCCCTGTTTCTGAGGAGGATGCCGTCGCCTCCGTTCGTGAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCTCCGTATGGAATTCGTAACTGTTATTTTCCTATTTACTTAGTGAGAGAGGCTTTCTTATGAAATTGACGAGTTTCTACATCAAACAATAGAGCTAGAGTTTGAGAATTTGTTCAATGCTTGGTCTTGCATGTTCATTTGTTTGGACTTGACTTTGTGCATGCTTGATTTTCGTTGAAATTTGTTGGCTCGATTTGGCTCTAGGAAGTTCTGTGTTGCTTTTTTGTTGAGATTTGTTTAGGTAAATGTAAGTTGTAACGAAGATTTGAAATATTGCTACGTGTATCCAGGTTTGATGTCTGATTTGATGTTATTGAAAGTTTAGATATGGAAGAGCCAATGGCTTTCGGAAAATATAAATCATTCCATTTTTTATATGTTTAGTTCATCGGTAAGGATATGTAAGGGCTAGTTTGGTTATCTTCTATATGATTTCTGGCTCTAGCACTGAATTTGGGTTTGCCTCTTCGACGACATGCGTGAAGGTACTATGGAGGGACCTTATCAGAAAAGATGCTTGGTAAGGGACTAAAAGCTCTAGATGTTCCGAGGAGTGAATATATAGTGTCAACAAAGTGTGGTAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTATGTCGATATACTGCATTGCCATGATATCGAATTTGGATCTCTTGATCAGGTTTGTTACTTTTCTTAAGAACTCATTGACCATTGAATTTTAGTTATTAACATGGTCATGTGACCTTGCATGGACAGGATTTGTCTTATAGGTTGTACAACTTTGTCCTTGAGTTCTAAAAAGCTATGAACATGGTTATTGGCATTATAATATCAAGTAAAAAGTGGATCTTCAGCCGCTAAAATTTATTTCAGGTTGTGAACGAAACGATTCCTGCACTTCAAAAGCTGAGGGAAGCTGGGAAGATTCGTTTCATTGGTATTACGGGACTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTGCCACCTGGCTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGATTCAACATTGTTAGATTTGCTACCTTACTTAAAGAGTAAGGAGATTGGAATAATCAGTGCGTCTCCTCTAGCAATGGGACTTCTAACTGATTATGGTCCCCCAGAATGGCACCCAGCTTCACCAGAATTGAAGGTACAGTTTCTCACCCCCCCCCTTCTTTCATTAGCTCCATAGTTGAATTGTGAAACTTAAAAGCTTATGCAGGGAAAGCACTTTCAGTTGACTGATGTCATGCAAAGAAAACACGTTTGTTTGGAGGAGTACATTTCTCGTTCTTTACTCTTATGGCGTTTAGGTCCCATCGTGGAAACTTTATCTCTTGAAATGAAAATAATTTCTAGGTGTTGATTATAGTATGCAGTAATGACTAGTTCATTCAACCATACAAACTAACCCAAAACCATTTGTTCTCGTGCTGTGATTTTATCTTTTATTTTTTCGAACTGTGTTTTGTTTGAGCTTTGGGTTTACATTGGATTCTGATATTGTTCTCTAATTTAAATGGCTTCAGTCTGCATGCCTAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAACATATTTCAAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGATATTTCAACGGTTCTGGTTGGCATGAACTCTGTCAGACAGGTATCTTTCAATAATCACATTTATAGTATTCTTTGTCGATATTTCTTTCAATATATCTTTCGAGTCCAATCTCCTTTTTCGAAGATTATGGCATGCCCCTATGACATGGTGTATATACTCATTCTGTATCAATTAGTTGGTAATTTCCTGGTCCATGCTTGTACTACTCCTACTTCGTTGTTGTAAAAACTTGCCTCCACACCAATGAGGAGGAAGTTCTTGTTTTCTGATATTGTTTATCTTTCATGGATAAAAAAAGTCAACCAGATTACTTCTTTATTCAACGAAAATATACCGATACAAGCAGACCACACAAGGTGGAGGGATAGGCAAAAAGAGAACTTTTGTATGGAAAGAATTGGGACAGCAAAGAGCAAGTTAAATGAACTTCTTTTTGATGGATGGATAGTTCCTAAAAGGTCATTAGACTATTCACCTTTGGATTTATGAACTGGTTCCTTCTTTTTGGTTGGAAATAAAAATTTAATTAGATAGAATAATTTTGGAGGGATCATCCCACACCATGACGTCAAAGGAGATTGATGACAGTCTGCATCCACTCTTGTTGGCTTTGATCAAGATTATAGCAGAATTGCAAAAATGGTTAGCAAGAGAGGACCAAGGAGAAAGAATGTTGGAGTTTCGTGTTGCTAGTTTGAATACTAATAGCACGAGAAACTAGTTTTTCGAAGTATAATTTTCATCCTATATTGCTACCTAGAATGTTAAAATCCTATGAGTTCACCAAATTGTTGAGGATTATTGGGAGTGAGTCCACGTTGGTTAATTTAGTGGAAGATCATGGTTTATAAGTGAGGAATATTATCTCCATTAGTATGAGGCCTTTTGGGGAAGTCCAAAAGCAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTCCTAACATGGTATCAGAGCCATGCCCTAAACTTAGCCATGACAATAGAATCCTCAAATATCGAATAAAGATTGTGAGCCTCTAAAGTGAAGTCAAAAAGTGACTAAAGTGTCGAACAAAAGGTGTACTTTGTTCGAGGGCTCCAAAGAAAGGAGTCGAGCCTCGATTAAGGGGAGGTTGTTCAAGAGCTCCATAAGCCTCAGGAGAGGCTTATAGTGTACTTTGTTCGAGGGGAGGATTGTTGAGGATTATTGGGAGTGAGTCTCACATTGGTTAATTTAGTGGAGGATCATAGGTTTATAAGTGAGGAATATTATCTCCATTGGTATGAGACCTTTTGGGGAAGCCCAAAGCAAATCCACGAGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTGGAGAGTCGTGAATATTTATTCTCATACAATGTTGAGCTCCCTTGGATTTAAGCCCTAAAAATCAATCTCGATGAGCTGACATATAGTGTTTGTTCATATTTTCTTCGTGACCATGCTGCAAACTTCTTCAAAGATTGCATCATAGAATCTTTAACCAAGTTGTAAAAATTTTGGAACAGGTGGAGGAAAACGTAGCTGCAGCTGAAGAACTTGCCACCTTTGGGAGGGATGAGGAAACTCTATCTGAGGTTGAAGCTATCCTTCACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

mRNA sequence

ATGGCAGCTTCTTATCCCAAGCTCCAGCTTCGGAAGCTGGGGAACACCGGCCTCAAAACCAGCAGTGTCGGCTTTGGTGCCTCGCCTCTTGGCAGCGTCTTTGGCCCTGTTTCTGAGGAGGATGCCGTCGCCTCCGTTCGTGAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCTCCGTACTATGGAGGGACCTTATCAGAAAAGATGCTTGGTAAGGGACTAAAAGCTCTAGATGTTCCGAGGAGTGAATATATAGTGTCAACAAAGTGTGGTAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTATGTCGATATACTGCATTGCCATGATATCGAATTTGGATCTCTTGATCAGGTTGTGAACGAAACGATTCCTGCACTTCAAAAGCTGAGGGAAGCTGGGAAGATTCGTTTCATTGGTATTACGGGACTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTGCCACCTGGCTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGATTCAACATTGTTAGATTTGCTACCTTACTTAAAGAGTAAGGAGATTGGAATAATCAGTGCGTCTCCTCTAGCAATGGGACTTCTAACTGATTATGGTCCCCCAGAATGGCACCCAGCTTCACCAGAATTGAAGTCTGCATGCCTAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAACATATTTCAAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGATATTTCAACGGTTCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGGAAAACGTAGCTGCAGCTGAAGAACTTGCCACCTTTGGGAGGGATGAGGAAACTCTATCTGAGGTTGAAGCTATCCTTCACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

Coding sequence (CDS)

ATGGCAGCTTCTTATCCCAAGCTCCAGCTTCGGAAGCTGGGGAACACCGGCCTCAAAACCAGCAGTGTCGGCTTTGGTGCCTCGCCTCTTGGCAGCGTCTTTGGCCCTGTTTCTGAGGAGGATGCCGTCGCCTCCGTTCGTGAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCTCCGTACTATGGAGGGACCTTATCAGAAAAGATGCTTGGTAAGGGACTAAAAGCTCTAGATGTTCCGAGGAGTGAATATATAGTGTCAACAAAGTGTGGTAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTATGTCGATATACTGCATTGCCATGATATCGAATTTGGATCTCTTGATCAGGTTGTGAACGAAACGATTCCTGCACTTCAAAAGCTGAGGGAAGCTGGGAAGATTCGTTTCATTGGTATTACGGGACTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTGCCACCTGGCTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGATTCAACATTGTTAGATTTGCTACCTTACTTAAAGAGTAAGGAGATTGGAATAATCAGTGCGTCTCCTCTAGCAATGGGACTTCTAACTGATTATGGTCCCCCAGAATGGCACCCAGCTTCACCAGAATTGAAGTCTGCATGCCTAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAACATATTTCAAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGATATTTCAACGGTTCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGGAAAACGTAGCTGCAGCTGAAGAACTTGCCACCTTTGGGAGGGATGAGGAAACTCTATCTGAGGTTGAAGCTATCCTTCACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

Protein sequence

MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSIDVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSACLAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEETLSEVEAILHPVKNQTWPSGIQQS
Homology
BLAST of Csor.00g157350 vs. ExPASy Swiss-Prot
Match: O81884 (L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 3.0e-152
Identity = 264/317 (83.28%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 7   KLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGT 66
           K++LR LGNTGLK S+VGFGASPLGSVFGPV+E+DAVA+VREAFRLGINFFDTSPYYGGT
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILH 126
           LSEKMLGKGLKAL VPRS+YIV+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDILH
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSIDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQKL++ GK RFIGITGLPL+IFTYVLDRVPPG++DVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSACLAAAAH 246
           CHY +NDSTLLDLLPYLKSK +G+ISASPLAMGLLT+ GPPEWHPASPELKSA  AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEETLSEVEA 306
           CK KGK I+KLALQYSL+NK+IS+VLVGM+SV QVEENVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILHPVKNQTWPSGIQQS 324
           IL PVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Csor.00g157350 vs. ExPASy Swiss-Prot
Match: Q04212 (D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ARA2 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-29
Identity = 90/292 (30.82%), Postives = 158/292 (54.11%), Query Frame = 0

Query: 46  VREAFRLGINFFDTSPYYGGTLSEKMLGKGLKAL--DVPRSEYIVSTKCGRY-AEGFDFS 105
           ++ AF  GIN  DTSPYYG   SE + G+ L  L  + PR  Y + TK GR  AE F++S
Sbjct: 41  IKYAFSHGINAIDTSPYYGP--SEVLYGRALSNLRNEFPRDTYFICTKVGRIGAEEFNYS 100

Query: 106 AERVTRSIDESLARLQLDYVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITG 165
            + V  S+  S  RL   Y+D+++ HD+EF     ++ E +  L+ L+  G I+  GI+G
Sbjct: 101 RDFVRFSVHRSCERLHTTYLDLVYLHDVEFVKFPDIL-EALKELRTLKNKGVIKNFGISG 160

Query: 166 LPLEIFTYVLDRVPP-----GSIDVILSYCHYSINDSTLLDLLPYL--KSKEIGIISASP 225
            P++  T++ +         GS+D +LSYC+ ++ ++ LL+    L   +K   + +AS 
Sbjct: 161 YPIDFITWLAEYCSTEESDIGSLDAVLSYCNLNLQNNKLLNFRERLLRNAKLKMVCNASI 220

Query: 226 LAMGLLTDYGPPEWHPASPELKSACLAAAAHCKKKGKHISKLALQYSLSN-KDISTVLVG 285
           L+M LL      ++HP S EL+     AA +C+++   ++ LA +Y++S       V++G
Sbjct: 221 LSMSLLRSQETRQFHPCSHELRECASQAAKYCQEQNVDLADLATRYAISEWVGKGPVVLG 280

Query: 286 MNSVRQVEENVAAAEELATFG-----RDEETLSEVEA-ILHPVKNQTWPSGI 321
           ++S+ +++  +   E + + G     +D + +  ++  I     N+ W SGI
Sbjct: 281 VSSMEELKLALDNYEIVKSNGNRLSSKDGQLVEYIQKNIFKEHFNEEWSSGI 329

BLAST of Csor.00g157350 vs. ExPASy Swiss-Prot
Match: Q52472 (D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 90/302 (29.80%), Postives = 146/302 (48.34%), Query Frame = 0

Query: 17  GLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGTLSEKMLGKGL 76
           GL   ++G+GA+ +G++F  +S+++A A +  A+  GI ++DT+P+YG  LSEK LG  L
Sbjct: 12  GLAIPALGYGAANVGNLFRALSDDEAWAVLEAAWDAGIRYYDTAPHYGLGLSEKRLGAFL 71

Query: 77  KALDVPRSEYIVSTKCGR------------------------YAEGFDFSAERVTRSIDE 136
           +    PR E++VSTK GR                            +DF+ + +  SI E
Sbjct: 72  QT--KPRDEFVVSTKAGRLLRPNPERRPSGLDTDNDFHVPDDLRREWDFTEQGIRASIAE 131

Query: 137 SLARLQLDYVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVL 196
           S  RL LD +D+L+ HD E   LD  +    PAL+K+R  G ++ IGI  +  +  T   
Sbjct: 132 SQERLGLDRIDLLYLHDPERHDLDLALASAFPALEKVRAEGVVKAIGIGSMVSDALTRA- 191

Query: 197 DRVPPGSIDVILSYCHYS-INDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPP---- 256
             V    +D+I+    Y+ +      ++LP       GI++AS    GLL    P     
Sbjct: 192 --VREADLDLIMVAGRYTLLEQPAATEVLPACAENATGIVAASVFNSGLLAQSEPKRDGR 251

Query: 257 -EWHPASPELKSACLAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVA 289
            E+     EL    +  AA C+     +   A+Q+ L +  + +V+VG +   Q+ +N  
Sbjct: 252 YEYGQLPDELWDRLVRIAAICRNHDVPLPAAAIQFPLQSALVRSVVVGGSRPAQLTQNAE 308

BLAST of Csor.00g157350 vs. ExPASy Swiss-Prot
Match: Q76KC2 (Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3)

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-20
Identity = 86/323 (26.63%), Postives = 142/323 (43.96%), Query Frame = 0

Query: 11  RKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGTLSEK 70
           R LG TGL  +++G G +PLG ++ PVS  DA A +   +  GI +FD++P YG    E 
Sbjct: 9   RALGRTGLTVTALGLGTAPLGGLYAPVSRADADALLEAGWDSGIRYFDSAPMYGYGRCEH 68

Query: 71  MLGKGLKALDVPRSEYIVSTKCGR-----------------------------YAEGFDF 130
           +LG  L+         ++STK GR                             + E FD+
Sbjct: 69  LLGDMLRE---KPERAVISTKVGRLMTNERAGRTLPPAPPKNPLDSGWHNGLNFREVFDY 128

Query: 131 SAERVTRSIDESLARLQLDYVDILHCHDI---------EFGSLDQVVNETIPALQKLREA 190
           S + V RS D+S  RL    +D+L+ HDI         EF            AL +LR A
Sbjct: 129 SYDGVMRSFDDSQQRLGFPEIDLLYVHDIGRVTHADRHEFHWNALTRGGGFRALTELRAA 188

Query: 191 GKIRFIGITGLPLEIFTYVLDRVPPGSIDVILSYCHYSINDS-TLLDLLPYLKSKEIGII 250
           G I+     GL +  +  + D +    +D  L    YS+ D  +  + LP  + + + ++
Sbjct: 189 GNIKGF---GLGVNEWQIIRDALEEADLDCSLLAGRYSLLDQVSEKEFLPLAQKRGMALV 248

Query: 251 SASPLAMGLLT--DYGPPEWHPASPELKSACLAAAAH--CKKKGKHISKLALQYSLSNKD 291
            A     G+L     G  ++  A    +        H  C +    ++  A+Q+ L ++ 
Sbjct: 249 IAGVFNSGILAAPRGGEQKFDYADAPAEIIARTNRLHDICDEYHVPLAAAAMQFPLRHEA 308

BLAST of Csor.00g157350 vs. ExPASy Swiss-Prot
Match: P46336 (Aldo-keto reductase IolS OS=Bacillus subtilis (strain 168) OX=224308 GN=iolS PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-20
Identity = 81/299 (27.09%), Postives = 137/299 (45.82%), Query Frame = 0

Query: 12  KLGNTGLKTSSVGFGASPLG--SVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGTLSE 71
           KLG + L+   +G G + +G  +++  ++EE     VREA R G+   DT+  YG   SE
Sbjct: 5   KLGKSDLQVFPIGLGTNAVGGHNLYPNLNEETGKELVREAIRNGVTMLDTAYIYGIGRSE 64

Query: 72  KMLGKGLKALDVPRSEYIVSTKCGRYAEG----FDFSAERVTRSIDESLARLQLDYVDIL 131
           +++G+ L+  +  R + +++TK     +G    FD S + + +S+DESL RL  DY+D+ 
Sbjct: 65  ELIGEVLREFN--REDVVIATKAAHRKQGNDFVFDNSPDFLKKSVDESLKRLNTDYIDLF 124

Query: 132 HCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSIDVILS 191
           + H   F       +E + AL ++++AGKIR IG++   LE    + +    G +DV+  
Sbjct: 125 YIH---FPDEHTPKDEAVNALNEMKKAGKIRSIGVSNFSLE---QLKEANKDGLVDVLQG 184

Query: 192 YCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLT------------DYGPPEWHPAS 251
             +  +N        PY K   I  I   PL  GLL             D    + H   
Sbjct: 185 E-YNLLNREAEKTFFPYTKEHNISFIPYFPLVSGLLAGKYTEDTTFPEGDLRNEQEHFKG 244

Query: 252 PELKSACLAA---AAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAE 290
              K         A   +K    I  + L + L+  +I  ++ G     Q+ +N+  A+
Sbjct: 245 ERFKENIRKVNKLAPIAEKHNVDIPHIVLAWYLARPEIDILIPGAKRADQLIDNIKTAD 294

BLAST of Csor.00g157350 vs. NCBI nr
Match: KAG6571895.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 635 bits (1638), Expect = 1.74e-229
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
           LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. NCBI nr
Match: XP_022952291.1 (L-galactose dehydrogenase [Cucurbita moschata])

HSP 1 Score: 633 bits (1632), Expect = 1.43e-228
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. NCBI nr
Match: KAG7011583.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 633 bits (1632), Expect = 7.91e-228
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS
Sbjct: 47  MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 106

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 107 PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 166

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 167 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 226

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 227 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 286

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 287 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 346

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 347 LSEVEAILHPVKNQTWPSGIQQS 369

BLAST of Csor.00g157350 vs. NCBI nr
Match: XP_023511604.1 (L-galactose dehydrogenase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629 bits (1621), Expect = 6.78e-227
Identity = 321/323 (99.38%), Postives = 321/323 (99.38%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAA CKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAAALCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. NCBI nr
Match: XP_022972473.1 (L-galactose dehydrogenase isoform X1 [Cucurbita maxima])

HSP 1 Score: 626 bits (1614), Expect = 7.91e-226
Identity = 319/323 (98.76%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG VSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGLVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. ExPASy TrEMBL
Match: A0A6J1GJU5 (L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 6.91e-229
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. ExPASy TrEMBL
Match: A0A6J1IA20 (L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025 PE=4 SV=1)

HSP 1 Score: 626 bits (1614), Expect = 3.83e-226
Identity = 319/323 (98.76%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG VSEEDAVASVREAFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGLVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAILHPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. ExPASy TrEMBL
Match: A0A6J1DMP0 (L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 2.09e-214
Identity = 301/323 (93.19%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILEPVKNQTWPSGIQQS 323

BLAST of Csor.00g157350 vs. ExPASy TrEMBL
Match: A0A1S3C0W3 (L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1)

HSP 1 Score: 587 bits (1513), Expect = 9.48e-211
Identity = 296/323 (91.64%), Postives = 309/323 (95.67%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MA SYPKL LR+LGNTGLKTSSVGFGASPLGSVF PVSEEDAVA+VREAFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKAL VPRSEYIVSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQ+VNETIPALQKL+EAGK RF+GITGLPLEIFTYVLDRVPPG+I
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSK +GIISASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAA HC+KKG++I+KLA+QYSL NKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAIL+PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Csor.00g157350 vs. ExPASy TrEMBL
Match: A0A5A7SPF2 (L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001760 PE=4 SV=1)

HSP 1 Score: 587 bits (1513), Expect = 9.48e-211
Identity = 296/323 (91.64%), Postives = 309/323 (95.67%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60
           MA SYPKL LR+LGNTGLKTSSVGFGASPLGSVF PVSEEDAVA+VREAFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKAL VPRSEYIVSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDILHCHDIEFGSLDQ+VNETIPALQKL+EAGK RF+GITGLPLEIFTYVLDRVPPG+I
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYLKSK +GIISASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300
            AAA HC+KKG++I+KLA+QYSL NKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILHPVKNQTWPSGIQQS 323
           LSEVEAIL+PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Csor.00g157350 vs. TAIR 10
Match: AT4G33670.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 539.3 bits (1388), Expect = 2.2e-153
Identity = 264/317 (83.28%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 7   KLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYYGGT 66
           K++LR LGNTGLK S+VGFGASPLGSVFGPV+E+DAVA+VREAFRLGINFFDTSPYYGGT
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILH 126
           LSEKMLGKGLKAL VPRS+YIV+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDILH
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSIDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQKL++ GK RFIGITGLPL+IFTYVLDRVPPG++DVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSACLAAAAH 246
           CHY +NDSTLLDLLPYLKSK +G+ISASPLAMGLLT+ GPPEWHPASPELKSA  AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEETLSEVEA 306
           CK KGK I+KLALQYSL+NK+IS+VLVGM+SV QVEENVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILHPVKNQTWPSGIQQS 324
           IL PVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Csor.00g157350 vs. TAIR 10
Match: AT1G60750.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.4e-21
Identity = 93/317 (29.34%), Postives = 145/317 (45.74%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG-PVSEEDAVASVREAFRLGINFFDT 60
           MA    +++  KLG+ GL+ S+ G G   L   +G P  E +AVA +R A   G+ F DT
Sbjct: 1   MAEEACQVRRMKLGSQGLEVSAQGLGCMGLSDFYGAPTPETNAVALLRHAINAGVTFLDT 60

Query: 61  SPYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAE-----GFDFSAERVTRSIDESL 120
           S  YG   +E +LGK LK  D  R +  ++TK G  A      GF    E V  + + SL
Sbjct: 61  SDIYGPETNELLLGKALK--DGLRDKVELATKFGITASEDGKFGFRGDPEYVRIACEASL 120

Query: 121 ARLQLDYVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDR 180
            RL +  +D+ + H I+       +  TI  L+KL E GKI++IG++             
Sbjct: 121 KRLGVTCIDLYYQHRID---TTLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAV 180

Query: 181 VPPGSIDVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASP 240
            P  ++ +  S     + +    D++P  +   IGI++ SPL  G L   G P +   + 
Sbjct: 181 HPITAVQIEWSLWSRDVEE----DIIPTCRELGIGIVAYSPLGRGFL---GLPRFQQENL 240

Query: 241 E-LKSACLAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELAT 300
           E  K       A   KK    ++LAL +     D    + G + ++ + +N+ A     T
Sbjct: 241 ENNKILYEKVQAMATKKSCTPAQLALAWVHHQGDDVCPIPGTSKIQNLNQNIGALSVKLT 300

Query: 301 FGRDEETLSEVEAILHP 311
                E + E+EAI  P
Sbjct: 301 ----PEEMVELEAIAQP 301

BLAST of Csor.00g157350 vs. TAIR 10
Match: AT1G04690.1 (potassium channel beta subunit 1 )

HSP 1 Score: 89.0 bits (219), Expect = 7.7e-18
Identity = 81/311 (26.05%), Postives = 140/311 (45.02%), Query Frame = 0

Query: 8   LQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFR-LGINFFDTSPYYGGT 67
           +Q + LG +GLK S++ FGA      FG   +     S+ +  R  G+NFFD +  Y   
Sbjct: 1   MQYKNLGKSGLKVSTLSFGA---WVTFGNQLDVKEAKSILQCCRDHGVNFFDNAEVYANG 60

Query: 68  LSEKMLGKGLKALDVPRSEYIVSTKC---GRYAEGFDFSAERVTRSIDESLARLQLDYVD 127
            +E+++G+ ++ L   RS+ ++STK    G        S + +      SL RL +DYVD
Sbjct: 61  RAEEIMGQAIRELGWRRSDIVISTKIFWGGPGPNDKGLSRKHIVEGTKASLKRLDMDYVD 120

Query: 128 ILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYV------LDRVPP 187
           +L+CH  +  +    + ET+ A+  + + G   + G +    +  T        LD V P
Sbjct: 121 VLYCHRPDAST---PIEETVRAMNYVIDKGWAFYWGTSEWSAQQITEAWGAADRLDLVGP 180

Query: 188 GSIDVILSYCHYSI--NDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHP---- 247
                I+    Y++        + LP   +  IG+ + SPLA G+LT        P    
Sbjct: 181 -----IVEQPEYNMFARHKVETEFLPLYTNHGIGLTTWSPLASGVLTGKYNKGAIPSDSR 240

Query: 248 ---------ASPELKSACLAAAAHCK----KKGKHISKLALQYSLSNKDISTVLVGMNSV 290
                    A+  L    L   +  K    + G  +++LA+ +  SN ++S+V+ G    
Sbjct: 241 FALENYKNLANRSLVDDVLRKVSGLKPIADELGVTLAQLAIAWCASNPNVSSVITGATRE 300

BLAST of Csor.00g157350 vs. TAIR 10
Match: AT1G59950.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.3e-04
Identity = 49/179 (27.37%), Postives = 77/179 (43.02%), Query Frame = 0

Query: 30  LGSVFGPVSEEDAV-ASVREAFRLGINFFDTSPYYGGTLSEKMLGKGLK-----ALDVPR 89
           LG+   P  E   +  +V EA +LG   FDTSP Y    +E+ LG+ L       L   R
Sbjct: 20  LGTAASPPPEPIVLKRTVLEAIKLGYRHFDTSPRY---QTEEPLGEALAEAVSLGLIQSR 79

Query: 90  SEYIVSTK--CGRYAEGFDFSAERVTRSIDESLARLQLDYVDILHCH------------D 149
           SE  V++K  C       D     V  +I  SL  L+LDY+D+   H             
Sbjct: 80  SELFVTSKLWCA------DAHGGLVVPAIQRSLETLKLDYLDLYLIHWPVSSKPGKYKFP 139

Query: 150 IEFGSLDQVVNETI-PALQKLREAGKIRFIGITGLPLEIFTYVLD--RVPPGSIDVILS 186
           IE      +  ET+   +++ +  G  + IG++    +   ++L   ++PP    V +S
Sbjct: 140 IEEDDFLPMDYETVWSEMEECQRLGVAKCIGVSNFSCKKLQHILSIAKIPPSVNQVEMS 189

BLAST of Csor.00g157350 vs. TAIR 10
Match: AT1G59960.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.9e-04
Identity = 43/132 (32.58%), Postives = 59/132 (44.70%), Query Frame = 0

Query: 6   PKLQLRK--LGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTSPYY 65
           P L +R    G+  +     G  ASPL     P   ++ V    EA +LG   FDTSP Y
Sbjct: 7   PTLAIRSGPSGHHSMPVLGFGTAASPLPE---PTMLKETVI---EAIKLGYRHFDTSPRY 66

Query: 66  GGTLSEKMLGKGLK-----ALDVPRSEYIVSTK--CGRYAEGFDFSAERVTRSIDESLAR 125
               +E+ +G+ L       L   RSE+ V+TK  C       D     V  +I  SL  
Sbjct: 67  ---QTEEPIGEALAEAVSLGLVRSRSEFFVTTKLWCA------DAHGGLVVPAIKRSLKN 123

Query: 126 LQLDYVDILHCH 129
           L+LDY+D+   H
Sbjct: 127 LKLDYLDLYIIH 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818843.0e-15283.28L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1[more]
Q042121.1e-2930.82D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Q524721.0e-2729.80D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1[more]
Q76KC21.2e-2026.63Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3[more]
P463361.6e-2027.09Aldo-keto reductase IolS OS=Bacillus subtilis (strain 168) OX=224308 GN=iolS PE=... [more]
Match NameE-valueIdentityDescription
KAG6571895.11.74e-229100.00L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022952291.11.43e-22899.69L-galactose dehydrogenase [Cucurbita moschata][more]
KAG7011583.17.91e-22899.69L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023511604.16.78e-22799.38L-galactose dehydrogenase isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022972473.17.91e-22698.76L-galactose dehydrogenase isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GJU56.91e-22999.69L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=... [more]
A0A6J1IA203.83e-22698.76L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025... [more]
A0A6J1DMP02.09e-21493.19L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV... [more]
A0A1S3C0W39.48e-21191.64L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1[more]
A0A5A7SPF29.48e-21191.64L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT4G33670.12.2e-15383.28NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60750.13.4e-2129.34NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G04690.17.7e-1826.05potassium channel beta subunit 1 [more]
AT1G59950.11.3e-0427.37NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G59960.12.9e-0432.58NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 23..289
e-value: 5.2E-56
score: 190.0
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 8..319
e-value: 7.0E-74
score: 250.7
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 8..309
NoneNo IPR availablePIRSRPIRSR000097-2PIRSR000097-2coord: 19..218
e-value: 6.6E-17
score: 59.2
NoneNo IPR availablePIRSRPIRSR000097-3PIRSR000097-3coord: 19..218
e-value: 6.6E-17
score: 59.2
NoneNo IPR availablePIRSRPIRSR000097-1PIRSR000097-1coord: 19..218
e-value: 6.6E-17
score: 59.2
IPR020471Aldo-keto reductasePANTHERPTHR42686GH17980P-RELATEDcoord: 7..320
IPR044479L-galactose dehydrogenase-likeCDDcd19163AKR_galDHcoord: 11..290
e-value: 1.13849E-177
score: 491.296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g157350.m01Csor.00g157350.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0010349 L-galactose dehydrogenase activity
molecular_function GO:0050235 pyridoxal 4-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity