Csor.00g156990 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g156990
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	cysteine proteinase inhibitor 5-like
Location	Csor_Chr19: 7655610 .. 7655969 (-)
RNA-Seq Expression	Csor.00g156990
Synteny	Csor.00g156990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGCTAACATCGGTCAACACGACCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGATCATTGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTAGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

mRNA sequence

ATGCTCGCTAACATCGGTCAACACGACCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGATCATTGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTAGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Coding sequence (CDS)

ATGCTCGCTAACATCGGTCAACACGACCCCATCCTCCATGTCACAAAGGATGCGAAGGGCGATCATTGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTAGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Protein sequence

MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE

Homology

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BLAST of Csor.00g156990 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.2e-07
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 26  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 85
           W  I ++    VQ+VA+F V E NK+     QY+ ++R +  + + G  YRL++  K GA
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYT-QVVAGTNYRLVIAAKDGA 91

Query: 86  SEGKYEAIVWE 97
             G YEA+VW+
Sbjct: 92  VVGNYEAVVWD 101

[Close]

BLAST of Csor.00g156990 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 4.9e-07
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0


Query: 26  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 85
           W  I  +    VQ+VA+F V E NK+     QY+ ++R +  + + G  YRL++  K GA
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYT-QVVAGTNYRLVIAAKDGA 91

Query: 86  SEGKYEAIVWE 97
             G YEA+VW+
Sbjct: 92  VVGNYEAVVWD 101

[Close]

BLAST of Csor.00g156990 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-04
Identity = 31/81 (38.27%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 32  IEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEV-KGGASEGKY 91
           I DP+VQE+ ++ V E ++   A       +    K+ + G+ YRL LE    GA +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 92  EAIVWEASGRLIIRKLKLVSF 112
           EA+V+E   R   R  KLVSF
Sbjct: 95  EAVVYEQDPRSNAR--KLVSF 113

[Close]

BLAST of Csor.00g156990 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 246 bits (627), Expect = 4.07e-82
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 0


Query: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60
           MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG
Sbjct: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60

Query: 61  LLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE 119
           LLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE
Sbjct: 61  LLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE 119

[Close]

BLAST of Csor.00g156990 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 187 bits (476), Expect = 5.29e-59
Identity = 97/120 (80.83%), Postives = 101/120 (84.17%), Query Frame = 0


Query: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60
           +LANIGQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF V E  KEK A  QYK
Sbjct: 5   VLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMARSQYK 64

Query: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGV 118
            LL CW+KEEMGGIKYRLILEV K    EGKYEAIVWEASGRLIIRKLKL+SF+ C HGV
Sbjct: 65  RLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHGV 124

[Close]

BLAST of Csor.00g156990 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 183 bits (465), Expect = 2.51e-57
Identity = 92/119 (77.31%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60
           +LAN+GQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF + E  KEK A FQYK
Sbjct: 5   LLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMARFQYK 64

Query: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHG 117
            LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+   HG
Sbjct: 65  RLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPSMHG 123

[Close]

BLAST of Csor.00g156990 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182 bits (462), Expect = 6.75e-57
Identity = 90/117 (76.92%), Postives = 97/117 (82.91%), Query Frame = 0


Query: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60
           +L NIGQHDPILHVTKD KGD  SEWISIR IEDPYVQEVAKF V+E N +K  TFQYK 
Sbjct: 5   LLTNIGQHDPILHVTKDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTTFQYKR 64

Query: 61  LLRCWVKEEMGGIKYRLILEVKGGASEGK---YEAIVWEASGRLIIRKLKLVSFVAC 114
           +LRCW+KEEMGGIKYRL+LEVK  A   K   YEA+VWEASGRLIIRKLKLVSFV C
Sbjct: 65  MLRCWIKEEMGGIKYRLVLEVKQEAPTQKVCKYEAVVWEASGRLIIRKLKLVSFVPC 121

[Close]

BLAST of Csor.00g156990 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.4 bits (176), Expect = 1.51e-13
Identity = 33/87 (37.93%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 10  PILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEE 69
           P+L V     G     W  I+ ++DP++QE+ KF V E NK+ K   ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 70  MGGIKYRLILEVKGGASEGKYEAIVWE 96
           + G+ YRL++E K G S GKY+A+VWE
Sbjct: 75  VSGMNYRLVIEAKKGESIGKYQALVWE 100

[Close]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 187 bits (476), Expect = 2.56e-59
Identity = 97/120 (80.83%), Postives = 101/120 (84.17%), Query Frame = 0


Query: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60
           +LANIGQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF V E  KEK A  QYK
Sbjct: 5   VLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMARSQYK 64

Query: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGV 118
            LL CW+KEEMGGIKYRLILEV K    EGKYEAIVWEASGRLIIRKLKL+SF+ C HGV
Sbjct: 65  RLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHGV 124

[Close]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 183 bits (465), Expect = 1.22e-57
Identity = 92/119 (77.31%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60
           +LAN+GQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF + E  KEK A FQYK
Sbjct: 5   LLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMARFQYK 64

Query: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHG 117
            LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+   HG
Sbjct: 65  RLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPSMHG 123

[Close]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.29e-14
Identity = 33/87 (37.93%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 10  PILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEE 69
           P+L V     G     W  I+ ++DP++QE+ KF V E NK+ K   ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 70  MGGIKYRLILEVKGGASEGKYEAIVWE 96
           + G+ YRL++E K G S GKY+A+VWE
Sbjct: 75  VSGMNYRLVIEAKKGESIGKYQALVWE 100

[Close]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.03e-13
Identity = 32/87 (36.78%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 10  PILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEE 69
           P+L V     G     W  I+ ++DP++QE+ KF V E NK+ K   ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 70  MGGIKYRLILEVKGGASEGKYEAIVWE 96
           + G+ YRL++E K G S GKY+A++WE
Sbjct: 75  VSGMNYRLVIEAKKGESIGKYQALIWE 100

[Close]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL
Match: A0A0B4ZWL9 (CPI-4 OS=Morus alba var. atropurpurea OX=1453101 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 8.59e-12
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 0


Query: 26 WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 85
          W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL+L VK GA
Sbjct: 29 WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVKGET-QVVAGTNYRLVLAVKNGA 88

Query: 86 SEGKYEAIVWE 96
          +  +YEA+VWE
Sbjct: 89 TAERYEAVVWE 98

The following BLAST results are available for this feature:

BLAST of Csor.00g156990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6TPK4	2.2e-07	38.03	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
P86472	4.9e-07	38.03	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q10Q47	1.7e-04	38.27	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of Csor.00g156990 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571859.1	4.07e-82	100.00	hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0031436.1	5.29e-59	80.83	Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystat...	[more]
KGN43767.1	2.51e-57	77.31	hypothetical protein Csa_017208 [Cucumis sativus]	[more]
KAG6588760.1	6.75e-57	76.92	hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022973098.1	1.51e-13	37.93	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SN35	2.56e-59	80.83	Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0K644	1.22e-57	77.31	Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE...	[more]
A0A6J1IC35	7.29e-14	37.93	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]
A0A6J1EKC1	1.03e-13	36.78	cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341...	[more]
A0A0B4ZWL9	8.59e-12	42.25	CPI-4 OS=Morus alba var. atropurpurea OX=1453101 PE=2 SV=1	[more]

BLAST of Csor.00g156990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 23..117 e-value: 1.2E-14 score: 56.1
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..112
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..112
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 22..111
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 29..98 e-value: 3.5E-12 score: 46.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g156990.m01	Csor.00g156990.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity