Csor.00g134170 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g134170
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	sterol 14-demethylase-like
Location	Csor_Chr14: 12958857 .. 12960485 (-)
RNA-Seq Expression	Csor.00g134170
Synteny	Csor.00g134170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCCGACAACAAATTCCTCAATGTGGGTCTTCTCGTGGTGGCCACTTTACTCCTCGCCAAGGTCATTTCTGCTGTTCTAAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTCAAGGGCTTCCCTCTGGTTGGGGGGTTGGTCCGTTTCCTCAAGGGTCCAATCGTGATGTTGAGGAAGGAGTACCCTGAGCTTGGAGCTGTCTTCACCTTGAACTTATTGCATAAGAAAATCACTTTCCTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTCGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATCCGCCAAGAACAGTTCAGATTCTTCACTGAGGCTCTCAGAGTCGCCAAATTGAAAGGCTATGTGGATCAGATGGTTACAGAAGCAGAGGTCAGTATTATTACTCCCCTTCTTCAATCATCTAAATTCATAAAGGACTATATTCATTTAATGCAGCCTCTGAGAATTTTATCTCCTTAAACATCCATTTATGTATCTCTTGAAACATCTAGTTCCTGAATCTGTGTAACCTTGGGATGTGGTGGGGTGTTTGGGCAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAATTGGAGCATCTTATCATCTTAACAGCTAGTAGATGCTTACTGGGTAGTGAGGTTCGTGATAAGCTATTCGCGGATGTATCCGCTTTGTTTCATGACCTTGACAATGGGATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCAGCTCATCGTCGCCGTGACAGAGCCCGCAGCAAGCTTGCAGATATCTTCGCCAAAATCATAGCTTCCAGAAAAAGCAGTGGCAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGCCAACATACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTCCTACGCCATAAAGAGTACATGTCTGCTGTTTTAGAGGAGCAGCAAAGGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGATAACCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACAACTAGAGAGGGGAAAGAATATGACATACCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTATGATCCAGACAGGTTTGCTCCTGGGCGAGATGAAGACAAGGCGGCCGGGCCATTCTCCTACATCTCGTTCGGAGGCGGTAGGCACGGGTGCCTCGGTGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAGTTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTGGGTGTCAAGGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAATTAA

mRNA sequence

ATGGAACCCGACAACAAATTCCTCAATGTGGGTCTTCTCGTGGTGGCCACTTTACTCCTCGCCAAGGTCATTTCTGCTGTTCTAAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTCAAGGGCTTCCCTCTGGTTGGGGGGTTGGTCCGTTTCCTCAAGGGTCCAATCGTGATGTTGAGGAAGGAGTACCCTGAGCTTGGAGCTGTCTTCACCTTGAACTTATTGCATAAGAAAATCACTTTCCTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTCGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATCCGCCAAGAACAGTTCAGATTCTTCACTGAGGCTCTCAGAGTCGCCAAATTGAAAGGCTATGTGGATCAGATGGTTACAGAAGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAATTGGAGCATCTTATCATCTTAACAGCTAGTAGATGCTTACTGGGTAGTGAGGTTCGTGATAAGCTATTCGCGGATGTATCCGCTTTGTTTCATGACCTTGACAATGGGATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCAGCTCATCGTCGCCGTGACAGAGCCCGCAGCAAGCTTGCAGATATCTTCGCCAAAATCATAGCTTCCAGAAAAAGCAGTGGCAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGCCAACATACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTCCTACGCCATAAAGAGTACATGTCTGCTGTTTTAGAGGAGCAGCAAAGGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGATAACCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACAACTAGAGAGGGGAAAGAATATGACATACCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTATGATCCAGACAGGTTTGCTCCTGGGCGAGATGAAGACAAGGCGGCCGGGCCATTCTCCTACATCTCGTTCGGAGGCGGTAGGCACGGGTGCCTCGGTGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAGTTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTGGGTGTCAAGGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAATTAA

Coding sequence (CDS)

ATGGAACCCGACAACAAATTCCTCAATGTGGGTCTTCTCGTGGTGGCCACTTTACTCCTCGCCAAGGTCATTTCTGCTGTTCTAAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTCAAGGGCTTCCCTCTGGTTGGGGGGTTGGTCCGTTTCCTCAAGGGTCCAATCGTGATGTTGAGGAAGGAGTACCCTGAGCTTGGAGCTGTCTTCACCTTGAACTTATTGCATAAGAAAATCACTTTCCTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAGGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTCGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATCCGCCAAGAACAGTTCAGATTCTTCACTGAGGCTCTCAGAGTCGCCAAATTGAAAGGCTATGTGGATCAGATGGTTACAGAAGCAGAGGACTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAATTGGAGCATCTTATCATCTTAACAGCTAGTAGATGCTTACTGGGTAGTGAGGTTCGTGATAAGCTATTCGCGGATGTATCCGCTTTGTTTCATGACCTTGACAATGGGATGCTCCCAATCAGTGTGATGTTCCCCTACCTTCCTATCCCAGCTCATCGTCGCCGTGACAGAGCCCGCAGCAAGCTTGCAGATATCTTCGCCAAAATCATAGCTTCCAGAAAAAGCAGTGGCAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATTCTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGCCAACATACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTCCTACGCCATAAAGAGTACATGTCTGCTGTTTTAGAGGAGCAGCAAAGGCTGATGGGAAAGCATGGCGATAAGATTGATCATGATATCTTGTCTGAAATGGATAACCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACAACTAGAGAGGGGAAAGAATATGACATACCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTATGATCCAGACAGGTTTGCTCCTGGGCGAGATGAAGACAAGGCGGCCGGGCCATTCTCCTACATCTCGTTCGGAGGCGGTAGGCACGGGTGCCTCGGTGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAGTTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCCATGGTTGTGGGTGTCAAGGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAATTAA

Protein sequence

MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVMLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSVN

Homology

[Close]

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot
Match: Q9SAA9 (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 3.4e-240
Identity = 402/486 (82.72%), Postives = 449/486 (92.39%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRS-NKRAPPTVKGF-PLVGGLVRFLKGPIV 60
           ++ +NK L  GL++VATL++AK+I +   S S  KR PPT+K + PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLR+EYP+LG+VFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYS+RQEQFRFFTEALRV KLKGYVD MVTEAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFA 240
           TASRCLLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK SG +ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA L+EQ+ L+ KHGDKIDHDILSEMD LYRCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFS 420
           +DFSVT R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

[Close]

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot
Match: P93846 (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.0e-223
Identity = 368/485 (75.88%), Postives = 432/485 (89.07%), Query Frame = 0


Query: 3   PDNKFLNVGL-LVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVMLR 62
           P  + L  GL LVVAT++  K++ +  +    KR PPT+ G P+VGGLV+F++GPI M+R
Sbjct: 7   PQQQRLMAGLALVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIR 66

Query: 63  KEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDV 122
           ++Y  LG+VFT+ ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDV
Sbjct: 67  EQYAALGSVFTVPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDV 126

Query: 123 DYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTAS 182
           DYS+RQEQFRFFTEALR  KL+ YVDQMV EAE++FSKWG+SG VDLKYELEHLIILTAS
Sbjct: 127 DYSVRQEQFRFFTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTAS 186

Query: 183 RCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKII 242
           RCLLG EVR+KLF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II
Sbjct: 187 RCLLGREVREKLFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATII 246

Query: 243 ASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLL 302
            SRK+SG SE DMLQCFIDSKYK+GRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L
Sbjct: 247 KSRKASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYML 306

Query: 303 RHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTDF 362
           R K+Y +  +EEQ+ +M +HGDKIDHDIL+EMD LYRCIKEALRLHPPLIML+R SH+DF
Sbjct: 307 RFKQYFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDF 366

Query: 363 SVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYIS 422
           +VTT+EGKEYDIPKGHI+ATSP+FANRLPH+YK+PD YDPDRF PGR+EDKAAG FSYIS
Sbjct: 367 TVTTKEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYIS 426

Query: 423 FGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRR 482
           FGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG+VMV YKRR
Sbjct: 427 FGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRR 486

Query: 483 KLSVN 487
           KL V+
Sbjct: 487 KLVVD 491

[Close]

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot
Match: P93596 (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.1e-219
Identity = 361/452 (79.87%), Postives = 412/452 (91.15%), Query Frame = 0


Query: 35  RAPPTVKGFPLVGGLVRFLKGPIVMLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFK 94
           R PPT+ G P+VGGL+RFL+GPI ++R EY  LG VFT+ +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 95  ASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAE 154
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY +RQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 155 DFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVM 214
           ++FSKWG+SG VDLKYELEHLIILTASRCLLG EVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 215 FPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEV 274
           FPYLPIPAHRRRD+AR++LA+IFA II SRK+SG SE DMLQCFIDSKYK+GR TT+SEV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 275 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMD 334
           TGLLIAALFAGQHTSSITSTWTGAYLL+ ++Y +  +EEQ+ +M +HGDKIDHDIL+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 335 NLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYK 394
            LYRCIKEALRLHPPLIML+R SH+DFSVTTREGKE+DIPKGHI+ATSPAFANRLPH++K
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 395 DPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELEL 454
           +PD YDPDRFA GR+EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 455 ISPFPEIDWNAMVVGVKGKVMVRYKRRKLSVN 487
           +SPFPE DWNAMVVG+KG+VMV YKRRKL V+
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKLIVD 452

[Close]

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot
Match: Q1ZXH9 (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 9.4e-105
Identity = 213/478 (44.56%), Postives = 295/478 (61.72%), Query Frame = 0


Query: 10  VGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVMLRKEYPELGA 69
           + +LV+A L    VI A   S SN   PP V+  P +G   +F K P+ ++R  Y  LG 
Sbjct: 5   IAVLVIAIL----VIFAFKKSPSN--IPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGE 64

Query: 70  VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQ 129
           +FTL+L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  I  EQ
Sbjct: 65  IFTLHLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQ 124

Query: 130 FRFFTEALRVAKLKGYVDQMVTEAEDFF-SKWGDSGEVDLKYELEHLIILTASRCLLGSE 189
            RF    L +++LK  V  +  E E +F +KWGDSGE+DL YE+  L ILTASRCL+G  
Sbjct: 125 LRFVKNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKS 184

Query: 190 VRDKL--FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIAS-RK 249
           +   L     ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+
Sbjct: 185 INKSLGQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRR 244

Query: 250 SSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKE 309
           S+  S +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + E
Sbjct: 245 STDDSVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLE 304

Query: 310 YMSAVLEEQQRLMGKHGD-KIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTDFSVT 369
           Y     ++   ++ K    +I+ D L  M+ L   I+E LRLHPPLI LMR       +T
Sbjct: 305 YFDETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMT 364

Query: 370 TREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGG 429
             E K   IP GHI+A SP    RLP VYK+PD ++P RF     EDK   PFS+I+FGG
Sbjct: 365 PMEYKGKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDV---EDKT--PFSFIAFGG 424

Query: 430 GRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRK 483
           G+HGC GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 GKHGCPGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

[Close]

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot
Match: Q4PJW3 (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.5e-91
Identity = 181/451 (40.13%), Postives = 260/451 (57.65%), Query Frame = 0


Query: 34  KRAPPTVKGFPLVGGLVRFLKGPIVMLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFF 93
           K  P  V   P +G  + F K PI  L   Y + G VF+  ++ K  T+L+G E +A  F
Sbjct: 58  KSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLF 117

Query: 94  KASESDLSQQEVY-QFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTE 153
            +   DL+ +EVY +   P FG GV +DV  ++  EQ +     L +A  + +V  +  E
Sbjct: 118 NSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKE 177

Query: 154 AEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPIS 213
            +++F  WG+SGE +L   L  LIILTAS CL G E+R +L   V+ L+ DLD G    +
Sbjct: 178 TKEYFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAA 237

Query: 214 VMFP-YLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSENDMLQCFIDSKYKDGRPTTD 273
            + P +LP+P+ RRRDRA  ++ +IF K I  R+ SG   +D+LQ  ++S YKDGRP TD
Sbjct: 238 WLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTD 297

Query: 274 SEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVLEEQQRLMGKHGDKIDHDILS 333
            EV G+LI  L AGQHTSS TS W G +L R K        EQ+ + G++   + +D L 
Sbjct: 298 DEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLK 357

Query: 334 EMDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEYDIPKGHIIATSPAFANRLPH 393
           +++ L RCIKE LRL PP++ +MR + T  +V       Y IP GH +  SP    RL  
Sbjct: 358 DLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA-----GYTIPPGHQVCVSPTVNQRLKD 417

Query: 394 VYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFE 453
            + +   ++PDR+    D   +   F+Y+ FG GRH C+GE FAY+QIK IWS +LR +E
Sbjct: 418 SWVERLDFNPDRYL--EDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYE 477

Query: 454 LELISP-FPEIDWNAMVVGVKGKVMVRYKRR 482
            +LI   FP +++  M +    K ++RYKRR
Sbjct: 478 FDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

[Close]

BLAST of Csor.00g134170 vs. NCBI nr
Match: XP_022955731.1 (sterol 14-demethylase-like [Cucurbita moschata] >KAG6581978.1 Sterol 14-demethylase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018397.1 Sterol 14-demethylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSVN
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Csor.00g134170 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 973 bits (2516), Expect = 0.0
Identity = 484/486 (99.59%), Postives = 485/486 (99.79%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYP+LGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRV KLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSVN
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Csor.00g134170 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 483/486 (99.38%), Postives = 485/486 (99.79%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYP+LGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRV KLKGYVDQMV+EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSVN
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Csor.00g134170 vs. NCBI nr
Match: XP_008454145.1 (PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethylase [Cucumis melo var. makuwa] >TYK29631.1 sterol 14-demethylase [Cucumis melo var. makuwa])

HSP 1 Score: 937 bits (2422), Expect = 0.0
Identity = 464/487 (95.28%), Postives = 478/487 (98.15%), Query Frame = 0


Query: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVN 486
           RRKLSV+
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of Csor.00g134170 vs. NCBI nr
Match: XP_038882542.1 (sterol 14-demethylase [Benincasa hispida])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 459/486 (94.44%), Postives = 475/486 (97.74%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           M+PDNKF NVGLL++ATL LAK+IS VLNSRSNKR PPTVKGFP +GGL+RFLKGPI+ML
Sbjct: 1   MDPDNKFFNVGLLILATLFLAKLISVVLNSRSNKRPPPTVKGFPFIGGLLRFLKGPIMML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYP+LG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYM+AVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+D
Sbjct: 301 LRHKEYMAAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           F+VTTREGKEYDIPKGHIIATSPAFANRL HVYKDP+RYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FTVTTREGKEYDIPKGHIIATSPAFANRLSHVYKDPNRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSV+
Sbjct: 481 RKLSVS 486

[Close]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL
Match: A0A6J1GUN6 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV=1)

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSVN
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL
Match: A0A6J1J0F8 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1)

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 483/486 (99.38%), Postives = 485/486 (99.79%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60
           MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  RKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           RKEYP+LGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRV KLKGYVDQMV+EAEDFFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420
           FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVN 486
           RKLSVN
Sbjct: 481 RKLSVN 486

[Close]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL
Match: A0A5A7TLX5 (Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002430 PE=3 SV=1)

HSP 1 Score: 937 bits (2422), Expect = 0.0
Identity = 464/487 (95.28%), Postives = 478/487 (98.15%), Query Frame = 0


Query: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVN 486
           RRKLSV+
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL
Match: A0A1S3BXE7 (sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1)

HSP 1 Score: 937 bits (2422), Expect = 0.0
Identity = 464/487 (95.28%), Postives = 478/487 (98.15%), Query Frame = 0


Query: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVN 486
           RRKLSV+
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL
Match: A0A0A0KYP3 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 461/487 (94.66%), Postives = 475/487 (97.54%), Query Frame = 0


Query: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60
           MEPDN KF NVGLLV+ATLLLAK+ISA+LNSRSNKR PPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAVLEEQQ LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVN 486
           RRKLSV+
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of Csor.00g134170 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 832.0 bits (2148), Expect = 2.4e-241
Identity = 402/486 (82.72%), Postives = 449/486 (92.39%), Query Frame = 0


Query: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRS-NKRAPPTVKGF-PLVGGLVRFLKGPIV 60
           ++ +NK L  GL++VATL++AK+I +   S S  KR PPT+K + PLVG L++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLR+EYP+LG+VFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYS+RQEQFRFFTEALRV KLKGYVD MVTEAED+FSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFA 240
           TASRCLLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK SG +ENDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA L+EQ+ L+ KHGDKIDHDILSEMD LYRCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFS 420
           +DFSVT R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

[Close]

BLAST of Csor.00g134170 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 127.1 bits (318), Expect = 3.9e-29
Identity = 116/465 (24.95%), Postives = 199/465 (42.80%), Query Frame = 0


Query: 18  LLLAKVISAVLNSRSNK-RAPPTVKGFPLVGGLVR-FLKGPIVMLRKEYPELGAVFTLNL 77
           L L + I+ V  S S K   PP   G+P VG   + + + P V    +    G+VF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 78  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 137
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 138 ALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 197
           A     ++  V  + + A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 198 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSE 257
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ + SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 258 NDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVL 317
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 318 EEQQ--RLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGK 377
           EEQ   R   K G+ +  +   +M   YR I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 378 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 437
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 436

Query: 438 GCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG 473
            C G   A L+I  +   L   +   ++ P   I +    +   G
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNG 454

[Close]

BLAST of Csor.00g134170 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 122.9 bits (307), Expect = 7.3e-28
Identity = 114/445 (25.62%), Postives = 193/445 (43.37%), Query Frame = 0


Query: 18  LLLAKVISAVLNSRSNK-RAPPTVKGFPLVGGLVR-FLKGPIVMLRKEYPELGAVFTLNL 77
           L L + I+ V  S S K   PP   G+P VG   + + + P V    +    G+VF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 78  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 137
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 138 ALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 197
           A     ++  V  + + A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 198 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSE 257
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ + SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 258 NDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVL 317
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 318 EEQQ--RLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGK 377
           EEQ   R   K G+ +  +   +M   YR I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 378 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 437
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 434

Query: 438 GCLGEPFAYLQIKAIWSQLLRNFEL 453
            C G   A L+I  +   L   + L
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRL 434

[Close]

BLAST of Csor.00g134170 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 115.9 bits (289), Expect = 8.9e-26
Identity = 104/455 (22.86%), Postives = 192/455 (42.20%), Query Frame = 0


Query: 37  PPTVKGFPLVGGLVR-FLKGPIVMLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKA 96
           PP   G+P VG   + + + P V  + +    G+VF  ++L      +  PE +A F   
Sbjct: 37  PPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPE-AAKFVLV 96

Query: 97  SESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAED 156
           ++S L +           G   +F        +  +    A     ++  V  + + A+D
Sbjct: 97  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 156

Query: 157 FFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF-ADVSALFHDLDNGMLPISVM 216
               W +   ++   E++      A   + G +  + L+  D+   ++ L+ G   + V 
Sbjct: 157 SLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRCYYILEKGYNSMPV- 216

Query: 217 FPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEV 276
              LP     +  +AR +L+ I A+I++ R+ +GSS ND+L  F+  K       TD ++
Sbjct: 217 --NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDK----EELTDEQI 276

Query: 277 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVLEEQQ--RLMGKHGDKIDHDILSE 336
              +I  +FA + T++   +W   YL  +   + AV EEQ   R   + G+ +      +
Sbjct: 277 ADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKK 336

Query: 337 MDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEYDIPKGHIIATSPAFAN--RLP 396
           M    R I+E LR+   L    R +  D      E + Y IPKG  +   P F N     
Sbjct: 337 MPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWKVL--PLFRNIHHSA 396

Query: 397 HVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNF 456
            ++ +P ++DP RF      + A  P +++ FG G H C G   A L++  +   L   +
Sbjct: 397 DIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 456

Query: 457 ELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 486
              ++     I +    +   G  +V  ++ ++ V
Sbjct: 457 SWSIVGASDGIQYGPFALPQNGLPIVLARKPEIEV 467

[Close]

BLAST of Csor.00g134170 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 111.7 bits (278), Expect = 1.7e-24
Identity = 100/420 (23.81%), Postives = 179/420 (42.62%), Query Frame = 0


Query: 37  PPTVKGFPLVGGLVR-FLKGPIVMLRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKA 96
           PP   G+P VG   + + + P V  + +    G+VF  ++L      +  PE +A F   
Sbjct: 37  PPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPE-AAKFVLV 96

Query: 97  SESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAED 156
           ++S L +           G   +F        +  +    A     ++  V  + + A+D
Sbjct: 97  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 156

Query: 157 FFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF-ADVSALFHDLDNGMLPISVM 216
               W +   ++   E++      A   + G +  + L+  D+   ++ L+ G   + V 
Sbjct: 157 SLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRCYYILEKGYNSMPV- 216

Query: 217 FPYLPIPAHRRRDRARSKLADIFAKIIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEV 276
              LP     +  +AR +L+ I A+I++ R+ +GSS ND+L  F+  K       TD ++
Sbjct: 217 --NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDK----EELTDEQI 276

Query: 277 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVLEEQQ--RLMGKHGDKIDHDILSE 336
              +I  +FA + T++   +W   YL  +   + AV EEQ   R   + G+ +      +
Sbjct: 277 ADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKK 336

Query: 337 MDNLYRCIKEALRLHPPLIMLMRSSHTDFSVTTREGKEYDIPKGHIIATSPAFAN--RLP 396
           M    R I+E LR+   L    R +  D      E + Y IPKG  +   P F N     
Sbjct: 337 MPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWKVL--PLFRNIHHSA 396

Query: 397 HVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNF 451
            ++ +P ++DP RF      + A  P +++ FG G H C G   A L++  +   L   +
Sbjct: 397 DIFSNPGKFDPSRF------EVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 432

The following BLAST results are available for this feature:

BLAST of Csor.00g134170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAA9	3.4e-240	82.72	Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1	[more]
P93846	1.0e-223	75.88	Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1	[more]
P93596	1.1e-219	79.87	Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP...	[more]
Q1ZXH9	9.4e-105	44.56	Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN...	[more]
Q4PJW3	3.5e-91	40.13	Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1	[more]

BLAST of Csor.00g134170 vs. NCBI nr


Match Name	Identity	Description
XP_022955731.1	100.00	sterol 14-demethylase-like [Cucurbita moschata] >KAG6581978.1 Sterol 14-demethyl...	[more]
XP_023527569.1	99.59	sterol 14-demethylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980879.1	99.38	sterol 14-demethylase-like [Cucurbita maxima]	[more]
XP_008454145.1	95.28	PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethyl...	[more]
XP_038882542.1	94.44	sterol 14-demethylase [Benincasa hispida]	[more]

BLAST of Csor.00g134170 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1GUN6	100.00	sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV...	[more]
A0A6J1J0F8	99.38	sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1	[more]
A0A5A7TLX5	95.28	Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65...	[more]
A0A1S3BXE7	95.28	sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1	[more]
A0A0A0KYP3	94.66	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1	[more]

BLAST of Csor.00g134170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11680.1	2.4e-241	82.72	CYTOCHROME P450 51G1	[more]
AT5G45340.1	3.9e-29	24.95	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G45340.2	7.3e-28	25.62	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	8.9e-26	22.86	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.2	1.7e-24	23.81	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 275..301 score: 37.32 coord: 413..429 score: 34.39 coord: 58..81 score: 30.61 coord: 371..385 score: 37.18 coord: 387..405 score: 28.34 coord: 429..447 score: 43.52 coord: 333..349 score: 46.61
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 284..301 score: 32.57 coord: 420..429 score: 40.95 coord: 338..349 score: 40.69 coord: 429..440 score: 38.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 37..456 e-value: 2.5E-66 score: 224.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..485 e-value: 1.3E-122 score: 411.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..481
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 10..481 e-value: 4.6E-33 score: 112.0
None	No IPR available	PANTHER	PTHR24286:SF251	STEROL 14-DEMETHYLASE	coord: 9..480
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 9..480
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 422..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g134170.m01	Csor.00g134170.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen