Csor.00g105570 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g105570
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr17: 8337167 .. 8340409 (+)
RNA-Seq ExpressionCsor.00g105570
SyntenyCsor.00g105570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGACAACGAGGTTGGAGAAAATGGCATCGTCGCCCGTGGCATCGTCGGAGATATTTAATGTGGATGATTATGGGGCAATGGGCGACGGAGAAGACGATAGCGAGGTGGTTTTTTTTTTTTTTTTTTTTTTATGAATAAAATTAATGAGTTTGAGTTAAAAGTAGTAGGAGTGGAAATGTTACGTATTTAATTATTTCAGGCGTTTAAAGAAGCTTGGAAGGAAGCTTGTTCGTCCACAAATGCCATCTTTTTGGTTCCTAATGATAGAACTTATCATCTTAAGCCAATCACTTTCTCCGGCCCTTGTAACTCTCCTTTTGCATTTAAGGTATATTTATGAGTATTAATATAATCCCTAAATTTTGTTAATTTTACAATAATTTTAAAATATTATTAAGATCCAATCATATTCCTTATTTCTTATCTATGTAATTAAAGATTTCATTATTTATAAATAATTTCTTTTAGTAATAACGAATGAGACAAGATATAAATTATAACATATTTTATTAGAGTAAGTTATTTTATTATTTTTTTTTTTTTTTTAAGGAAGAAATTTCTATTTTCCTTTCTTATATGATTTATTTGAATTTATGTAATTATATTGAATTAAATTAATAATATTTCAAACATTAAAATCTTTGTAATAAAGTTATCATAAATTCCTTTTGTATTTATTAATATATTTATTTAATATAGTTAACGGCTTTTTTAAAATTTCTTCAATTGCATTTCAACCCTCCATTTTATTACTATTATTATTATTAGCAATTTATATTTCGGTTTAACAAATATTTATTATTTAAATTTTGACTTTATCTTCTTTCATATAATATCACAATTATTTAGGAAACTGAAATATTCTGGTATTTTTGGAAAAATGAAGATTAAGTTTTTTTTAATTAAAAATGAATTAATATTATTAATTTCTCTTAATAATTACTTTTTTTTTTTTTTTTAATTGGAAGATTGAAGGGACAATAAAAGCCTCCCCACATATATCAGATTACGAGAAAGACAGAAGGCATTGGATTATATTTGAAAATCTATCAGAATTTACCGTGGAAGGTAAAGGCACCATTAATGGAAATGGAAGAAAATGGTGGTTAAATTCATGCAAAGTCAACAAAACTCTAGTGAGTTTCTCAATTTTACCTCAGTAAATTTTCAATCACAACTCATTTTGTGTGTTAATGGATTTCTTCTTCCATTTTTCTTGTTCTTTCAGCCCTGCAAGGAAGCTCCAACAGTAAGGCTTGTGTGATTTTGAATCTTTGGAAGAAATAAGTGCGAGATGGTTTATAACAAAAAAAAAAAAAATAATAATAATACTCTCTCCATTGGTATGAGGTCTTTTGGGGAAGCCCAAAGCGAGCTTATGCTCAAAGTGGAGCATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACAATAAGAACATCTCCATTAACTTTGAAGCTAATTTCTCTCTCTCTCTCTCTAGGCTGTTACTTTCTATCAATGTACCCATCTGAGAGTGGAAGGGCTGAGGTTCAGAAATGCACAAAAGATGCATCTTTCCTTTCAGAAATGCAGCAATGTCAAAGCTCTAAATCTATGGATTTATGCACCAGGAAACAGCCCCAACACTGATGGCATTCATGTCACAGCCACACAAATCATTCTCATCCAAAACTGTCTTATAATGACTGGTAGGGTACTCCTATACCTAAAAGCTTCATAACTTTTACTTCATAACTTTATCTTTGTACTCTTTTTTTCAAGGTGATGACTGTATTTCGATCGTAAGTGGGTCGAAAAATGTTCGAGCAAAGGGCATAACTTGTGGACCGGGCCATGGGATCAGGTTTCCATAATGCAGCTCTACAACTTGGCTAATTTGATTCATTGGGGTCTGTTTTATTGACTGTTTTTTTTCTGTTTCTGGTTTGTTTTGCAGTATTGGAAGCTTGGGAGCTGGAAAATCAGAAGCTGAGGTTTCTAATGTGGTGGTTGATACAGCAAAGTTTACAGGAACATCAAATGGAGTGAGAATCAAAACTTGGCAGGTATACAAAGAAAACTTGTTTAGAACAGTAAAGATCGGTTGTTTTGAGTTGAAAATTGAAGCTATATTTCACTTTGTTAAGACTCAAAAATCAGAAAATGATGGAAGTTTACTGGATGCAAAAGCAACAGTGTTCGTGCCTGCTGATTTTGACACCAGAACTTGACCTGCACATTACTTAATCCTCTACTTTTTGACCAACTCAAAACCTGTATGAGTATAGGTATCATGTGCTTTTCAAACCTACCAAACAATTCTCAGTTTTTTTTCACAAGTCAATGGCATTCAGGAGGGAGGGGGAAGTTTGGTTTGCTTTCTCCCCTGCTAGTCAGCTAAGAATTGGGCTTTGAACAGATTTCAAACATAGTGAACCAGTAAGATGAACATATTATGGAAGTTTCTGCACAAGAGAAAGATAAACAGCAAAAGAAATCAATGGGCCATGCCTTGCTACTCTTTCTTTGATCTTCTTTATTTATTTCAAGTCTTGTTTTATTTTATTGGGTTTTTAACTTCTGCATCTTGTTTGCGTTGGTTCTTTAGGTTCAAGTTTGGTCTACAAACTTTGTGCAAAATAATTGTTTTCTTCTTTCATTAGAAGTCAAATAAATAACAAAATTGATAACTTCAAGACAATTTCAACTTAGGAAACACAACTTAAGAGTTAGATTTAATGTTTTGATTTGTCTTAAACTATCTATCAAAGATCTCTTTAGTTCTAATCCAACAGTTTGGTGTACAGGGAGGCAAAGGGTATGCAGAAAACATAATATTCCAGAACATTGTGATGGACAATGTGACCAACCCAATAATCATAGATCAAAACTACTGTGATCAGAAAGAACCATGTACACAACAGGTAAACACTTCAAAAACCCAAATCAGCACAGCCTACAGCTTCAATATTCACGAAAAATTTCTGCTAAGCATTTTATCAAACACGTAACAGGCAGAAGCAGTACAAGTGAGTAATGTAATGTACCAGAACATCAGAGGCACAAGCGGTTCGGAAGTGGCTGTGAAATTCGATTGCAGCAAAAGCTTCCCATGTCAGGAGATTCTTCTACAAGACATCGATCTTGTCCACAGAGATAAAGCAGACGATAAACCCACCCAAGCCGAAGCTTCCTGTAAAAATGCCAGGTTGAAGAATAGAGGAAGGGTTTCTCCACAGTGCCCTGGACGACGATGA

mRNA sequence

ATGTTGACAACGAGGTTGGAGAAAATGGCATCGTCGCCCGTGGCATCGTCGGAGATATTTAATGTGGATGATTATGGGGCAATGGGCGACGGAGAAGACGATAGCGAGGCGTTTAAAGAAGCTTGGAAGGAAGCTTGTTCGTCCACAAATGCCATCTTTTTGGTTCCTAATGATAGAACTTATCATCTTAAGCCAATCACTTTCTCCGGCCCTTGTAACTCTCCTTTTGCATTTAAGATTGAAGGGACAATAAAAGCCTCCCCACATATATCAGATTACGAGAAAGACAGAAGGCATTGGATTATATTTGAAAATCTATCAGAATTTACCGTGGAAGCCACACAAATCATTCTCATCCAAAACTGTCTTATAATGACTGGTGATGACTGTATTTCGATCGTAAGTGGGTCGAAAAATGTTCGAGCAAAGGGCATAACTTGTGGACCGGGCCATGGGATCAGTATTGGAAGCTTGGGAGCTGGAAAATCAGAAGCTGAGGTTTCTAATGTGGTGGTTGATACAGCAAAGTTTACAGGAACATCAAATGGAGTGAGAATCAAAACTTGGCAGGGAGGCAAAGGGTATGCAGAAAACATAATATTCCAGAACATTGTGATGGACAATGTGACCAACCCAATAATCATAGATCAAAACTACTGTGATCAGAAAGAACCATGTACACAACAGGCAGAAGCAGTACAAGTGAGTAATGTAATGTACCAGAACATCAGAGGCACAAGCGGTTCGGAAGTGGCTGTGAAATTCGATTGCAGCAAAAGCTTCCCATGTCAGGAGATTCTTCTACAAGACATCGATCTTGTCCACAGAGATAAAGCAGACGATAAACCCACCCAAGCCGAAGCTTCCTGTAAAAATGCCAGGTTGAAGAATAGAGGAAGGGTTTCTCCACAGTGCCCTGGACGACGATGA

Coding sequence (CDS)

ATGTTGACAACGAGGTTGGAGAAAATGGCATCGTCGCCCGTGGCATCGTCGGAGATATTTAATGTGGATGATTATGGGGCAATGGGCGACGGAGAAGACGATAGCGAGGCGTTTAAAGAAGCTTGGAAGGAAGCTTGTTCGTCCACAAATGCCATCTTTTTGGTTCCTAATGATAGAACTTATCATCTTAAGCCAATCACTTTCTCCGGCCCTTGTAACTCTCCTTTTGCATTTAAGATTGAAGGGACAATAAAAGCCTCCCCACATATATCAGATTACGAGAAAGACAGAAGGCATTGGATTATATTTGAAAATCTATCAGAATTTACCGTGGAAGCCACACAAATCATTCTCATCCAAAACTGTCTTATAATGACTGGTGATGACTGTATTTCGATCGTAAGTGGGTCGAAAAATGTTCGAGCAAAGGGCATAACTTGTGGACCGGGCCATGGGATCAGTATTGGAAGCTTGGGAGCTGGAAAATCAGAAGCTGAGGTTTCTAATGTGGTGGTTGATACAGCAAAGTTTACAGGAACATCAAATGGAGTGAGAATCAAAACTTGGCAGGGAGGCAAAGGGTATGCAGAAAACATAATATTCCAGAACATTGTGATGGACAATGTGACCAACCCAATAATCATAGATCAAAACTACTGTGATCAGAAAGAACCATGTACACAACAGGCAGAAGCAGTACAAGTGAGTAATGTAATGTACCAGAACATCAGAGGCACAAGCGGTTCGGAAGTGGCTGTGAAATTCGATTGCAGCAAAAGCTTCCCATGTCAGGAGATTCTTCTACAAGACATCGATCTTGTCCACAGAGATAAAGCAGACGATAAACCCACCCAAGCCGAAGCTTCCTGTAAAAATGCCAGGTTGAAGAATAGAGGAAGGGTTTCTCCACAGTGCCCTGGACGACGATGA

Protein sequence

MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHRDKADDKPTQAEASCKNARLKNRGRVSPQCPGRR
Homology
BLAST of Csor.00g105570 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.9e-84
Identity = 172/375 (45.87%), Postives = 211/375 (56.27%), Query Frame = 0

Query: 16  SSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGPCNSP 75
           +S+  NVDD+GA GDG DD++AF++AWK ACSST++  L+   + Y ++PI+FSGPC S 
Sbjct: 86  ASKTVNVDDFGAKGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 145

Query: 76  FAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE----------------------- 135
              +I GTI+AS   SDY KD RHW++F+++    VE                       
Sbjct: 146 LTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL 205

Query: 136 ------------------------------------------------------------ 195
                                                                       
Sbjct: 206 PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIH 265

Query: 196 --ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNV 255
              TQ I I +C+I TGDDCISIV+GS+ VR   ITCGPGHGISIGSLG G SEA VS+V
Sbjct: 266 VTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDV 325

Query: 256 VVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQA 306
           VV+ AK  GT+NGVRIKTWQGG G A NI FQN+ M NV NPIIIDQNYCDQ +PC +Q+
Sbjct: 326 VVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQS 385

BLAST of Csor.00g105570 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-82
Identity = 176/381 (46.19%), Postives = 222/381 (58.27%), Query Frame = 0

Query: 12  SPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV-PNDRTYHLKPITFSG 71
           +P +S   FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P +R Y LK +TFSG
Sbjct: 63  APRSSPRSFNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFSG 122

Query: 72  PCNSP-FAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE----------------- 131
           PC S    FKI G I+A  + SDY K+RRHWI+FEN++   VE                 
Sbjct: 123 PCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSC 182

Query: 132 ------------------------------------------------------------ 191
                                                                       
Sbjct: 183 KINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSP 242

Query: 192 --------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSE 251
                    TQ ILIQ+ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG   SE
Sbjct: 243 NTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSE 302

Query: 252 AEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKE 306
           A VSNVVV+ A   GT+NGVRIKTWQGG G A+NIIFQ+I+M NVTNPIII+Q+YCD+ E
Sbjct: 303 AYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVE 362

BLAST of Csor.00g105570 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-75
Identity = 166/375 (44.27%), Postives = 209/375 (55.73%), Query Frame = 0

Query: 18  EIFNVDDYGAMGDGED-DSEAFKEAWKEACSSTNAI-FLVPNDRTYHLKPITFSGPCNSP 77
           ++ NV  +GA GDG+  D+ AF++AW EACSS   + F+VP ++ Y LK ITFSGPC S 
Sbjct: 74  KVINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFSGPCRSS 133

Query: 78  FAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFT------------------------- 137
            + KI G+++AS  ISDY KDRR WI F+++                             
Sbjct: 134 ISVKIFGSLEASSKISDY-KDRRLWIAFDSVQNLVVGGGGTINGNGQVWWPSSCKINKSL 193

Query: 138 ------------------------------------------------------------ 197
                                                                       
Sbjct: 194 PCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFESCTNVVASNLMINASAKSPNTDGVH 253

Query: 198 VEATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNV 257
           V  TQ I I + +I TGDDCISIVSGS+NV+A  ITCGPGHGISIGSLG+G SEA VSNV
Sbjct: 254 VSNTQYIQISDTIIGTGDDCISIVSGSQNVQATNITCGPGHGISIGSLGSGNSEAYVSNV 313

Query: 258 VVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQA 306
            V+ AK  G  NGVRIKTWQGG G A NI F N+ M +V  PIIIDQNYCD+ EPC QQ 
Sbjct: 314 TVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVEPCIQQF 373

BLAST of Csor.00g105570 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.6e-73
Identity = 160/340 (47.06%), Postives = 192/340 (56.47%), Query Frame = 0

Query: 21  NVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGPCNSPFAFKI 80
           +VDD+GA GDG DD++AF++AWK+ACSS  ++ +VP ++ Y LK ITFSGPC S    KI
Sbjct: 86  SVDDFGARGDGTDDTKAFEKAWKDACSS-GSVLIVPENKNYLLKQITFSGPCKSDLRVKI 145

Query: 81  EGTIKASPHISDY-EKDRRHWIIFENLSEFTVE--------------------------- 140
            GTI+AS   SD+   +R+ WI FE++S  T+E                           
Sbjct: 146 RGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPCKS 205

Query: 141 ----------------------------------------------------------AT 200
                                                                      T
Sbjct: 206 APTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHITGT 265

Query: 201 QIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVVDT 260
           Q I + N +I TGDDCISI SGSK V A  ITCGPGHGISIGSLG   SEA VS V+VD 
Sbjct: 266 QRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLVDG 325

Query: 261 AKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQAEAVQ 275
                T+NG+RIKTWQGG G A+NI FQNIVM NVTNPIIIDQ YCD K+PC +Q  AV+
Sbjct: 326 GNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVK 385

BLAST of Csor.00g105570 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.3e-71
Identity = 162/378 (42.86%), Postives = 204/378 (53.97%), Query Frame = 0

Query: 15  ASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGPCNS 74
           A ++  +VDD+GA G+G DD++AF +AWK ACSS+ A+ LV   + Y ++PI FSGPC S
Sbjct: 94  APAKTISVDDFGAKGNGADDTQAFVKAWKAACSSSGAMVLVVPQKNYLVRPIEFSGPCKS 153

Query: 75  PFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTV----------------------- 134
               +I GTI+AS   S Y KD  HW+IF+N+    V                       
Sbjct: 154 QLTLQIYGTIEASEDRSIY-KDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCKIKPQ 213

Query: 135 ---------------------------EATQI-ILIQNCL-------------------- 194
                                      +A QI ++ QNC+                    
Sbjct: 214 PPCGTYAPTAVTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDG 273

Query: 195 ---------------IMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVS 254
                          I TGDDCISIVSGS+ V+A  ITCGPGHGISIGSLG   SE  VS
Sbjct: 274 IHVTNTQNITISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVS 333

Query: 255 NVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQK-EPCT 306
            V V+ AK +GTSNG+RIKTW+GG G A NI+FQN+ M++VTNPIIIDQNYCD K + C 
Sbjct: 334 GVFVNGAKLSGTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDCK 393

BLAST of Csor.00g105570 vs. NCBI nr
Match: KAG6575692.1 (Polygalacturonase QRT2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623 bits (1607), Expect = 3.11e-225
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT
Sbjct: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEATQIILIQ 120
           YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEATQIILIQ
Sbjct: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEATQIILIQ 120

Query: 121 NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVVDTAKFTGT 180
           NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVVDTAKFTGT
Sbjct: 121 NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVVDTAKFTGT 180

Query: 181 SNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMY 240
           SNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMY
Sbjct: 181 SNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMY 240

Query: 241 QNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHRDKADDKPTQAEASCKNARLKNRGR 300
           QNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHRDKADDKPTQAEASCKNARLKNRGR
Sbjct: 241 QNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHRDKADDKPTQAEASCKNARLKNRGR 300

Query: 301 VSPQCPGRR 309
           VSPQCPGRR
Sbjct: 301 VSPQCPGRR 309

BLAST of Csor.00g105570 vs. NCBI nr
Match: XP_022953263.1 (polygalacturonase QRT2-like [Cucurbita moschata])

HSP 1 Score: 581 bits (1498), Expect = 3.05e-206
Identity = 307/394 (77.92%), Postives = 308/394 (78.17%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVP DRT
Sbjct: 66  MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPIDRT 125

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE-------- 120
           YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE        
Sbjct: 126 YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKGTINGN 185

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 186 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNL 245

Query: 181 -----------------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                            ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 246 WIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 305

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII
Sbjct: 306 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 365

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCS+SFPCQEILLQDIDLVH
Sbjct: 366 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQDIDLVH 425

BLAST of Csor.00g105570 vs. NCBI nr
Match: XP_023547692.1 (polygalacturonase QRT2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580 bits (1494), Expect = 1.07e-205
Identity = 304/394 (77.16%), Postives = 307/394 (77.92%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDS+AFKEAWKEACSSTNAIFLVPNDRT
Sbjct: 62  MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSQAFKEAWKEACSSTNAIFLVPNDRT 121

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE-------- 120
           YHLKPITFSGPCNSPF FKIEGTIKASPHISDYEKDRRHWI+FENL+EFTVE        
Sbjct: 122 YHLKPITFSGPCNSPFVFKIEGTIKASPHISDYEKDRRHWIVFENLAEFTVEGKGTINGN 181

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 182 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNL 241

Query: 181 -----------------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                            ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 242 WIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 301

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVSNVVVDTAKF GTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII
Sbjct: 302 GSLGAGKSEAEVSNVVVDTAKFKGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 361

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH
Sbjct: 362 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 421

BLAST of Csor.00g105570 vs. NCBI nr
Match: XP_022991888.1 (polygalacturonase QRT2-like [Cucurbita maxima])

HSP 1 Score: 573 bits (1476), Expect = 7.30e-203
Identity = 302/394 (76.65%), Postives = 305/394 (77.41%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMA SPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT
Sbjct: 68  MLTTRLEKMAPSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 127

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE-------- 120
           YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENL+EFTVE        
Sbjct: 128 YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEGKGIINGN 187

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 188 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNL 247

Query: 181 -----------------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                            ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 248 WIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 307

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVS+VVVDTAKFTGTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIII
Sbjct: 308 GSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIII 367

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPC EILLQDIDLVH
Sbjct: 368 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILLQDIDLVH 427

BLAST of Csor.00g105570 vs. NCBI nr
Match: KAG7014247.1 (hypothetical protein SDJN02_24422, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 537 bits (1384), Expect = 7.67e-190
Identity = 284/333 (85.29%), Postives = 286/333 (85.89%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVP DRT
Sbjct: 66  MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPIDRT 125

Query: 61  YHLKPITFSGPCNSPFAFK--------IEGTIKAS------------PHISDYEKDRRHW 120
           YHLKPITFSGPCNSPFAFK          G+ K +             H     K    W
Sbjct: 126 YHLKPITFSGPCNSPFAFKPCKEAPTVFWGSPKRAYAQSGAYHTIVESHKCSNVKALNLW 185

Query: 121 IIFENLSEFT----VEATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIG 180
           I     S  T    V ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIG
Sbjct: 186 IYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIG 245

Query: 181 SLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIID 240
           SLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIID
Sbjct: 246 SLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIID 305

Query: 241 QNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHR 300
           QNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHR
Sbjct: 306 QNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVHR 365

Query: 301 DKADDKPTQAEASCKNARLKNRGRVSPQCPGRR 309
           DKADDKPTQAEASCKNARLKNRGRVSPQCPGRR
Sbjct: 366 DKADDKPTQAEASCKNARLKNRGRVSPQCPGRR 398

BLAST of Csor.00g105570 vs. ExPASy TrEMBL
Match: A0A6J1GMX8 (polygalacturonase QRT2-like OS=Cucurbita moschata OX=3662 GN=LOC111455865 PE=3 SV=1)

HSP 1 Score: 581 bits (1498), Expect = 1.48e-206
Identity = 307/394 (77.92%), Postives = 308/394 (78.17%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVP DRT
Sbjct: 66  MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPIDRT 125

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE-------- 120
           YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE        
Sbjct: 126 YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKGTINGN 185

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 186 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNL 245

Query: 181 -----------------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                            ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 246 WIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 305

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII
Sbjct: 306 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 365

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCS+SFPCQEILLQDIDLVH
Sbjct: 366 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQDIDLVH 425

BLAST of Csor.00g105570 vs. ExPASy TrEMBL
Match: A0A6J1JXJ9 (polygalacturonase QRT2-like OS=Cucurbita maxima OX=3661 GN=LOC111488393 PE=3 SV=1)

HSP 1 Score: 573 bits (1476), Expect = 3.53e-203
Identity = 302/394 (76.65%), Postives = 305/394 (77.41%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           MLTTRLEKMA SPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT
Sbjct: 68  MLTTRLEKMAPSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 127

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE-------- 120
           YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENL+EFTVE        
Sbjct: 128 YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEGKGIINGN 187

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 188 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALNL 247

Query: 181 -----------------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                            ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 248 WIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 307

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVS+VVVDTAKFTGTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIII
Sbjct: 308 GSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIII 367

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPC EILLQDIDLVH
Sbjct: 368 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILLQDIDLVH 427

BLAST of Csor.00g105570 vs. ExPASy TrEMBL
Match: O81246 (Polygalacturonase OS=Cucumis melo OX=3656 GN=MPG3 PE=2 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 3.57e-177
Identity = 267/392 (68.11%), Postives = 282/392 (71.94%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           ML TRLEKM SSP+AS EIFNVDDYGAMGDGEDD+EAFKE WK+ACSSTNAIFLVP DR 
Sbjct: 69  MLRTRLEKMVSSPLASPEIFNVDDYGAMGDGEDDTEAFKETWKDACSSTNAIFLVPCDRV 128

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEA------- 120
           YHLKPITFSGPCNSP  F+IEGT+KASPHISDYEKDRRHWIIF+NL    VE        
Sbjct: 129 YHLKPITFSGPCNSPLLFQIEGTVKASPHISDYEKDRRHWIIFQNLLGLRVEGKGIINGN 188

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 189 GRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQQMLLSFQRCNNVKALNL 248

Query: 181 ------------------TQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                             TQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 249 WIYAPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 308

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVSNVVVDTAKF+GTSNGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIII
Sbjct: 309 GSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIII 368

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 307
           +QNYCDQKEPCTQQA+AV VSNVMYQNIRGTS SEVAVKFDCSKS PCQ ILLQDI+LVH
Sbjct: 369 NQNYCDQKEPCTQQADAVAVSNVMYQNIRGTSASEVAVKFDCSKSVPCQGILLQDINLVH 428

BLAST of Csor.00g105570 vs. ExPASy TrEMBL
Match: A0A6J1CWK1 (polygalacturonase QRT2 OS=Momordica charantia OX=3673 GN=LOC111015052 PE=3 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 6.40e-174
Identity = 259/394 (65.74%), Postives = 284/394 (72.08%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           +LTT+LE+ A SPVAS EIFNVDD+GAMGDGEDDSEAFKEAWKEACSSTN I LVP +RT
Sbjct: 61  VLTTKLERGAPSPVASPEIFNVDDFGAMGDGEDDSEAFKEAWKEACSSTNGILLVPYERT 120

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEA------- 120
           YHLKPITFSGPCNSP  FKI+GTIKASPH+SDYEKDRRHWI+F+N+ +FTV+        
Sbjct: 121 YHLKPITFSGPCNSPLVFKIDGTIKASPHLSDYEKDRRHWIVFQNMEDFTVKGDGTINGN 180

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 181 GRKWWLNSCKVNKTLPCKDAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALNL 240

Query: 181 ------------------TQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                             TQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI
Sbjct: 241 WIYAPGNSPNTDGIHVTGTQFIVIENCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 300

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 300
           GSLGAGKSEAEVSNVVVDTAKF+GTSNGVRIKTWQGGKGYA+NIIFQNIVM+NV+NPIII
Sbjct: 301 GSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMNNVSNPIII 360

Query: 301 DQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLVH 309
           DQNYCD+KE C QQA+AVQVSNV Y+NIRGTS SEVAVKFDCSKS PCQ ILLQD++LVH
Sbjct: 361 DQNYCDRKERCKQQADAVQVSNVKYRNIRGTSASEVAVKFDCSKSLPCQGILLQDVNLVH 420

BLAST of Csor.00g105570 vs. ExPASy TrEMBL
Match: A0A0A0K6J9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G427620 PE=3 SV=1)

HSP 1 Score: 394 bits (1011), Expect = 3.72e-133
Identity = 212/339 (62.54%), Postives = 230/339 (67.85%), Query Frame = 0

Query: 1   MLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPNDRT 60
           ML TRLEKMASSP+AS EIFNVDDYGAMGDGEDDSEAFKE WK+ACSSTNAIFLVP DR 
Sbjct: 66  MLRTRLEKMASSPLASPEIFNVDDYGAMGDGEDDSEAFKETWKDACSSTNAIFLVPCDRV 125

Query: 61  YHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEA------- 120
           YHLKPITF+GPCNSP  FKIEGTIKASPHISDYEKDRRHWIIF+NL    VE        
Sbjct: 126 YHLKPITFTGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNLLGLRVEGKGIINGN 185

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 186 GRKWWLNSCKVNKTLPCKEAPTAVTFFQCTNLRVEGLRFRNAQQMHLSFQKCNNVKALNL 245

Query: 181 ------------------TQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISI 240
                             TQ I+I+NCLIMTGDDC+SIVSGSKNVRAKGITCGPGHGISI
Sbjct: 246 WIHSPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCLSIVSGSKNVRAKGITCGPGHGISI 305

Query: 241 GSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIII 254
           GSLGAGK+EAEVSNVVVDTAKF+GT+NGVRIKTWQGGKGYA+NIIFQNIVMDNVTNPIII
Sbjct: 306 GSLGAGKTEAEVSNVVVDTAKFSGTTNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPIII 365

BLAST of Csor.00g105570 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.3e-83
Identity = 176/381 (46.19%), Postives = 222/381 (58.27%), Query Frame = 0

Query: 12  SPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV-PNDRTYHLKPITFSG 71
           +P +S   FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P +R Y LK +TFSG
Sbjct: 63  APRSSPRSFNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFSG 122

Query: 72  PCNSP-FAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVE----------------- 131
           PC S    FKI G I+A  + SDY K+RRHWI+FEN++   VE                 
Sbjct: 123 PCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSC 182

Query: 132 ------------------------------------------------------------ 191
                                                                       
Sbjct: 183 KINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSP 242

Query: 192 --------ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSE 251
                    TQ ILIQ+ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG   SE
Sbjct: 243 NTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSE 302

Query: 252 AEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKE 306
           A VSNVVV+ A   GT+NGVRIKTWQGG G A+NIIFQ+I+M NVTNPIII+Q+YCD+ E
Sbjct: 303 AYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVE 362

BLAST of Csor.00g105570 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-68
Identity = 159/391 (40.66%), Postives = 203/391 (51.92%), Query Frame = 0

Query: 4   TRLEKMASSPVASSEIFNVDDYGAMGDGE-DDSEAFKEAWKEACSSTNAI-FLVPNDRTY 63
           T L     + + +    +V D+GA GDG+ DD++AF  AWK+ACSS  A+  LVP   TY
Sbjct: 52  TGLRSFVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTY 111

Query: 64  HLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEA-------- 123
            LK I  +GPCNS    +I GT+ AS   SDY KD   WI+F+ ++  +V+         
Sbjct: 112 LLKSIQLTGPCNSILTVQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVDG 171

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 172 NGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSN 231

Query: 184 -------------------TQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 243
                              TQ I +   +I TGDDCISI SGS+NV+   ITCGPGHGIS
Sbjct: 232 VVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGIS 291

Query: 244 IGSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPII 303
           IGSLG   S+A VS V VD AK +GT NGVRIKT+QGG G A NIIFQNI MDNV NPII
Sbjct: 292 IGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPII 351

Query: 304 IDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLV 306
           IDQ+YCD K  CT +  AVQV NV+Y++I GTS SE A+ F+CSK++PCQ I+L  +++ 
Sbjct: 352 IDQDYCD-KSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI- 411

BLAST of Csor.00g105570 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-67
Identity = 154/375 (41.07%), Postives = 204/375 (54.40%), Query Frame = 0

Query: 21  NVDDYGAMGDGE-DDSEAFKEAWKEACSSTNAI--FLVPNDRTYHLKPITFSGPCNSPFA 80
           +V ++GA GDG+ DD++AFK+AWK+AC STN +  FLVP  +TY LK   F GPC S   
Sbjct: 69  SVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYLLKSTRFRGPCKSLRN 128

Query: 81  FKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEA------------------------ 140
           F+I GT+ AS   SDY KD+ HW+I E+++  +++                         
Sbjct: 129 FQILGTLSASTKRSDY-KDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKSK 188

Query: 141 ------------------------------------------------------------ 200
                                                                       
Sbjct: 189 PCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIH 248

Query: 201 ---TQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNV 260
              TQ I + N  I TGDDCISI  G++N++   +TCGPGHGISIGSLG   S+A VS +
Sbjct: 249 ITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGI 308

Query: 261 VVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCTQQA 306
            VD AKF+ + NGVRIKT+QGG G A+NI FQNI M+NV NPIIIDQ+YCD K+ C  Q 
Sbjct: 309 NVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQE 368

BLAST of Csor.00g105570 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 8.6e-59
Identity = 140/376 (37.23%), Postives = 183/376 (48.67%), Query Frame = 0

Query: 16  SSEIFNVDDYGAMGDG-EDDSEAFKEAWKEACSS-TNAIFLVPNDRTYHLKPITFSGPCN 75
           S  + +V ++GA G+G  DD++AF +AWK ACSS      LVP + T  L+PI  SGPC 
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 76  SPFAFKIEGTIKASPHISDYE-KDRRHWIIFENLSEFTVE-------------------- 135
           +    +I GTI A      +E  +RR W+ F  LS  TVE                    
Sbjct: 109 ARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHN 168

Query: 136 ------------------------------------------------------------ 195
                                                                       
Sbjct: 169 HSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTD 228

Query: 196 -----ATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEV 255
                 ++ I+I N  + TGDDCISIV  S  +    I CGPGHGISIGSLG  KS  EV
Sbjct: 229 GIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEV 288

Query: 256 SNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCT 304
            ++ VDTA  + T+NGVRIKTWQGG G    IIF+NI M+NV+NPIIIDQ YCD ++PC 
Sbjct: 289 RDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCA 348

BLAST of Csor.00g105570 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 218.8 bits (556), Expect = 6.2e-57
Identity = 131/376 (34.84%), Postives = 184/376 (48.94%), Query Frame = 0

Query: 15  ASSEIFNVDDYGAMGDG-EDDSEAFKEAWKEACSSTNAIFLVPNDRTYHLKPITFSGPCN 74
           +   + NVD +GA GDG  DD++AF  AW +ACS++ ++FLVP  R Y +    F+GPC 
Sbjct: 62  SGKNLVNVDTFGAAGDGVSDDTQAFVSAWSKACSTSKSVFLVPEGRRYLVNATKFNGPCE 121

Query: 75  SPFAFKIEGTIKASPHISDYE-KDRRHWIIFENL-------------------------- 134
                +I+GTI A    S+++ K +R W+ F  L                          
Sbjct: 122 QKLIIQIDGTIVAPDEPSNWDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKKN 181

Query: 135 ----------------------SEFTVEATQ----------------------------- 194
                                 S  T++ +Q                             
Sbjct: 182 KSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNTD 241

Query: 195 --------IILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEV 254
                    +++Q+C I TGDDC+SIV+ S N++ K I CGPGHGISIGSLG   +   V
Sbjct: 242 GIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGIV 301

Query: 255 SNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPIIIDQNYCDQKEPCT 304
           + VV+DTA    T+NG+RIKT+QGG GY + I F N+ M +V NPI+IDQ YCD    C 
Sbjct: 302 TQVVLDTALLRETTNGLRIKTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTCQ 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P353369.9e-8445.87Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q9SFB71.9e-8246.19Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
P051171.3e-7544.27Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1[more]
Q020964.6e-7347.06Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
P489784.3e-7142.86Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6575692.13.11e-225100.00Polygalacturonase QRT2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022953263.13.05e-20677.92polygalacturonase QRT2-like [Cucurbita moschata][more]
XP_023547692.11.07e-20577.16polygalacturonase QRT2-like [Cucurbita pepo subsp. pepo][more]
XP_022991888.17.30e-20376.65polygalacturonase QRT2-like [Cucurbita maxima][more]
KAG7014247.17.67e-19085.29hypothetical protein SDJN02_24422, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A6J1GMX81.48e-20677.92polygalacturonase QRT2-like OS=Cucurbita moschata OX=3662 GN=LOC111455865 PE=3 S... [more]
A0A6J1JXJ93.53e-20376.65polygalacturonase QRT2-like OS=Cucurbita maxima OX=3661 GN=LOC111488393 PE=3 SV=... [more]
O812463.57e-17768.11Polygalacturonase OS=Cucumis melo OX=3656 GN=MPG3 PE=2 SV=1[more]
A0A6J1CWK16.40e-17465.74polygalacturonase QRT2 OS=Momordica charantia OX=3673 GN=LOC111015052 PE=3 SV=1[more]
A0A0A0K6J93.72e-13362.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G427620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07970.11.3e-8346.19Pectin lyase-like superfamily protein [more]
AT2G41850.11.2e-6840.66polygalacturonase abscission zone A. thaliana [more]
AT3G57510.11.7e-6741.07Pectin lyase-like superfamily protein [more]
AT1G80170.18.6e-5937.23Pectin lyase-like superfamily protein [more]
AT1G23460.16.2e-5734.84Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 109..291
e-value: 1.5E-58
score: 198.4
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 144..157
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..112
e-value: 3.9E-18
score: 67.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 113..291
e-value: 1.3E-62
score: 213.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 279..309
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 12..116
coord: 111..295
NoneNo IPR availablePANTHERPTHR31375:SF167POLYGALACTURONASE QRT2coord: 12..116
coord: 111..295
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 8..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g105570.m01Csor.00g105570.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity