Csor.00g094760 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g094760 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.7e-161
Identity = 287/417 (68.82%), Postives = 339/417 (81.29%), Query Frame = 0

Query: 38  SSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 97
           SS +  L+LPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 98  WLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLS 157
           WL CNRS N +  ++  F+P  SSSY+PIPCSS TC  RTRDF IPASCDS+ LCHATLS
Sbjct: 100 WLRCNRSSNPNPVNN--FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 159

Query: 158 YADASSSEGTLATDTFYIGNS-GISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 217
           YADASSSEG LA + F+ GNS   SN++FGCM S+  S+ EED+K TGL+GMNRGSLSF+
Sbjct: 160 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 219

Query: 218 SQMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLE 277
           SQMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL 
Sbjct: 220 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLT 279

Query: 278 GIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVF 337
           GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLNRT G   V+
Sbjct: 280 GIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVY 339

Query: 338 EDSNFVFQGAMDLCYRVPMNQTR---LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGN 397
           ED +FVFQG MDLCYR+   + R   L  LP+V+LVF GAE+ V+G  +LYRVP    GN
Sbjct: 340 EDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGN 399

Query: 398 DSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 450
           DS++CFTFGNSDL+G+EA+VIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 400 DSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Csor.00g094760 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.8e-39
Identity = 117/370 (31.62%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 75  VSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCT 134
           ++L++GTP Q  + ++DTGS+L W  C       + S+  FNP GSSS++ +PCSS  C 
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 135 DRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSS 194
                  + +   SN+ C  T  Y D S ++G++ T+T   G+  I N+ FGC       
Sbjct: 157 ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GE 216

Query: 195 NNE--EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPL 254
           NN+        GL+GM RG LS  SQ+   KFSYC++    S    LLLG    S  A  
Sbjct: 217 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 276

Query: 255 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFE-PDHTGAGQTMVDSGTQF 314
             T LI+ S+ +P F    Y + L G+ V    LPI  S F    + G G  ++DSGT  
Sbjct: 277 PNTTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 336

Query: 315 TFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVF 374
           T+ +  AY ++R EF+++   +  V   S+  F    DLC++ P + + L  +P+  + F
Sbjct: 337 TYFVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVMHF 396

Query: 375 RGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKK 434
            G ++ +  +         I  ++ + C   G+S   G+  F  G++ QQN+ + +D   
Sbjct: 397 DGGDLELPSENYF------ISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGN 434

Query: 435 SRIGLAEIRC 440
           S +  A  +C
Sbjct: 457 SVVSFASAQC 434

BLAST of Csor.00g094760 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.5e-37
Identity = 107/367 (29.16%), Postives = 174/367 (47.41%), Query Frame = 0

Query: 75  VSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCT 134
           +++ +GTP  + + ++DTGS+L W  C       S  +  FNP  SSS++ +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 135 DRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSS 194
           D      +P+   +N+ C  T  Y D S+++G +AT+TF    S + N+ FGC +    +
Sbjct: 158 D------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DN 217

Query: 195 NNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPLNY 254
                    GL+GM  G LS  SQ+G  +FSYC++ Y  S    L LG A          
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 255 TPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFL 314
           T LI  S    Y     Y + L+GI V    L IP S F+    G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 315 LGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGA 374
              AY A+   F ++   +    ++S+      +  C++ P + + +  +P +++ F G 
Sbjct: 338 PQDAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFDGG 397

Query: 375 EMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRI 434
            + +    IL      I   + + C   G+S  LG+  F  G++ QQ   + +DL+   +
Sbjct: 398 VLNLGEQNIL------ISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 435 GLAEIRC 440
                +C
Sbjct: 458 SFVPTQC 435

BLAST of Csor.00g094760 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.2e-32
Identity = 123/372 (33.06%), Postives = 181/372 (48.66%), Query Frame = 0

Query: 77  LTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDR 136
           L VGTP + V MV+DTGS++ WL C   +   S S   F+P  S +Y  IPCSS  C  R
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHC--R 205

Query: 137 TRDFPIPASCDSNH-LCHATLSYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSN 196
             D    A C++    C   +SY D S + G  +T+T     + +  V  GC       N
Sbjct: 206 RLD---SAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDN 265

Query: 197 NEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYDFS---GLLLLGDANFSWLAP 256
                   GL+G+ +G LSF  Q G     KFSYC+ +   S     ++ G+A  S +A 
Sbjct: 266 EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIA- 325

Query: 257 LNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLP-IPESVFEPDHTGAGQTMVDSGTQ 316
             +TPL+      P  D   Y V L GI V    +P +  S+F+ D  G G  ++DSGT 
Sbjct: 326 -RFTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 385

Query: 317 FTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVP-MNQTRLPPLPSVTL 376
            T L+ PAY A+RD F     G+  +    +F      D C+ +  MN+ +   +P+V L
Sbjct: 386 VTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDTCFDLSNMNEVK---VPTVVL 445

Query: 377 VFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDL 436
            FRGA++++      Y +P +  G     CF F  + + G+   +IG++ QQ   + +DL
Sbjct: 446 HFRGADVSLPATN--YLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDL 484

Query: 437 KKSRIGLAEIRC 440
             SR+G A   C
Sbjct: 506 ASSRVGFAPGGC 484

BLAST of Csor.00g094760 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.2e-31
Identity = 127/395 (32.15%), Postives = 182/395 (46.08%), Query Frame = 0

Query: 72  SLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSS 131
           S  V   +G+P Q + + +DT ++ +W HC  S   +  SSS F PA SSSY  +PCSSS
Sbjct: 78  SYVVRAGLGSPSQQLLLALDTSADATWAHC--SPCGTCPSSSLFAPANSSSYASLPCSSS 137

Query: 132 TC-----------TDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 191
            C                  P PA+  +   C  +  +ADA S +  LA+DT  +G   I
Sbjct: 138 WCPLFQGQACPAPQGGGDAAPPPATLPT---CAFSKPFADA-SFQAALASDTLRLGKDAI 197

Query: 192 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYD---FS 251
            N  FGC+ S+  +    +    GL+G+ RG ++ +SQ G      FSYC+  Y    FS
Sbjct: 198 PNYTFGCVSSV--TGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFS 257

Query: 252 GLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPD 311
           G L LG A       + YTP++      P+   + Y V + G+ V H  + +P   F  D
Sbjct: 258 GSLRLG-AGGGQPRSVRYTPMLRN----PHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFD 317

Query: 312 -HTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVP 371
             TGAG T+VDSGT  T    P Y ALR+EF  + A        S +   GA D C+   
Sbjct: 318 AATGAG-TVVDSGTVITRWTAPVYAALREEFRRQVAA------PSGYTSLGAFDTCFNT- 377

Query: 372 MNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA--- 431
            ++      P+VT+   G         +   +P E   N  IH      + L   EA   
Sbjct: 378 -DEVAAGGAPAVTVHMDGG--------VDLALPME---NTLIHSSATPLACLAMAEAPQN 437

Query: 432 -----FVIGHLHQQNVWMEFDLKKSRIGLAEIRCD 441
                 VI +L QQN+ + FD+  SR+G A+  C+
Sbjct: 438 VNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

BLAST of Csor.00g094760 vs. NCBI nr
Match: XP_022935018.1 (aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of Csor.00g094760 vs. NCBI nr
Match: XP_022934632.1 (uncharacterized protein LOC111441767 [Cucurbita moschata])

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 186 MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 245

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 246 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 305

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 306 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 365

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 366 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 425

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 426 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 485

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 486 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 545

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 546 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 605

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 606 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 635

BLAST of Csor.00g094760 vs. NCBI nr
Match: XP_023528618.1 (aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 448/450 (99.56%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTD+TRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of Csor.00g094760 vs. NCBI nr
Match: XP_023528617.1 (aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 448/450 (99.56%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 55  MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 114

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 115 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 174

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTD+TRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 175 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 234

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 235 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 294

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 295 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 354

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 355 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 414

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 415 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 474

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 475 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 504

BLAST of Csor.00g094760 vs. NCBI nr
Match: XP_022983486.1 (aspartic proteinase PCS1 [Cucurbita maxima])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 444/450 (98.67%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKS FPFFLC LFSVFQNLILCSS NPALLLPLKTQVIPPESIRR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSS TFNPAGS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSS-TFNPAGS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTD+TRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGS RVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

BLAST of Csor.00g094760 vs. ExPASy TrEMBL
Match: A0A6J1F9C1 (aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1)

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of Csor.00g094760 vs. ExPASy TrEMBL
Match: A0A6J1F3C7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1)

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR
Sbjct: 186 MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 245

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS
Sbjct: 246 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 305

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 306 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 365

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 366 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 425

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 426 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 485

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 486 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 545

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 546 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 605

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 606 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 635

BLAST of Csor.00g094760 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 444/450 (98.67%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYCIAFNSSNHKFLKS FPFFLC LFSVFQNLILCSS NPALLLPLKTQVIPPESIRR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSS TFNPAGS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSS-TFNPAGS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSYTPIPCSSSTCTD+TRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLNRTAGS RVFEDSNFVFQGAMDLCYRVPMNQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

BLAST of Csor.00g094760 vs. ExPASy TrEMBL
Match: A0A0A0LAI7 (Aspartic proteinase nepenthesin-2 OS=Cucumis sativus OX=3659 GN=Csa_3G734110 PE=3 SV=1)

HSP 1 Score: 827 bits (2135), Expect = 2.87e-301
Identity = 414/450 (92.00%), Postives = 427/450 (94.89%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MRDYC AFN S+ KFLKS F FF CTLFSVF ++ LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCFAFNFSSVKFLKSCFLFFFCTLFSVFHSIHLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMVIDTGSELSWLHCN SQNSSSSSS TFNP  S
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSS-TFNPVWS 120

Query: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180
           SSY+PIPCSSSTCTD+TRDFPI  SCDSN  CHATLSYADASSSEG LATDTFYIG+SGI
Sbjct: 121 SSYSPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGSSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NVVFGCMDSIFSSN+EEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 PNVVFGCMDSIFSSNSEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKV+HKLLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRD FLN+TAGS RV+EDSNFVFQGAMDLCYRVP NQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDHFLNKTAGSLRVYEDSNFVFQGAMDLCYRVPTNQTRLP 360

Query: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVFRGAEMTVTGDRILYRVPGE RGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

BLAST of Csor.00g094760 vs. ExPASy TrEMBL
Match: A0A5D3DN23 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001570 PE=3 SV=1)

HSP 1 Score: 827 bits (2135), Expect = 3.09e-301
Identity = 414/451 (91.80%), Postives = 427/451 (94.68%), Query Frame = 0

Query: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60
           MR YC+AF+S+N KFLKS F FF C LFSVF N+ LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRGYCLAFDSANIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSS-TFNPAG 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMVIDTGSELSWLHCN SQNSSSSSSS TFNP  
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVR 120

Query: 121 SSSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSG 180
           SSSY+PIPCSSSTCTD+TRDFPI  SCDSN  CHATLSYADASSSEG LATDTFYIGNSG
Sbjct: 121 SSSYSPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSG 180

Query: 181 ISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240
           I NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL
Sbjct: 181 IPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240

Query: 241 GDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAG 300
           GDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKV+HKLLPIPESVFEPDHTGAG
Sbjct: 241 GDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAG 300

Query: 301 QTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRL 360
           QTMVDSGTQFTFLLGPAYTALRD FLN+TAGS R++ED NFVFQGAMDLCYRVP NQTRL
Sbjct: 301 QTMVDSGTQFTFLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQTRL 360

Query: 361 PPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQ 420
           PPLPSVTLVFRGAEMTVTGDRILYRVPGE RGNDSIHCFTFGNSDLLGVEAFVIGHLHQQ
Sbjct: 361 PPLPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQ 420

Query: 421 NVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450
           NVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 NVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 451

BLAST of Csor.00g094760 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 570.5 bits (1469), Expect = 1.2e-162
Identity = 287/417 (68.82%), Postives = 339/417 (81.29%), Query Frame = 0

Query: 38  SSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 97
           SS +  L+LPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 98  WLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLS 157
           WL CNRS N +  ++  F+P  SSSY+PIPCSS TC  RTRDF IPASCDS+ LCHATLS
Sbjct: 100 WLRCNRSSNPNPVNN--FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 159

Query: 158 YADASSSEGTLATDTFYIGNS-GISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 217
           YADASSSEG LA + F+ GNS   SN++FGCM S+  S+ EED+K TGL+GMNRGSLSF+
Sbjct: 160 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 219

Query: 218 SQMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLE 277
           SQMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL 
Sbjct: 220 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLT 279

Query: 278 GIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVF 337
           GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLNRT G   V+
Sbjct: 280 GIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVY 339

Query: 338 EDSNFVFQGAMDLCYRVPMNQTR---LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGN 397
           ED +FVFQG MDLCYR+   + R   L  LP+V+LVF GAE+ V+G  +LYRVP    GN
Sbjct: 340 EDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGN 399

Query: 398 DSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 450
           DS++CFTFGNSDL+G+EA+VIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 400 DSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Csor.00g094760 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 535.4 bits (1378), Expect = 4.4e-152
Identity = 268/415 (64.58%), Postives = 321/415 (77.35%), Query Frame = 0

Query: 38  SSQNPALLLPLKTQVIPPESIRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 97
           SS N  LL  LKTQ +P    + S DKL FRHNV+LTV+L VG PPQN++MV+DTGSELS
Sbjct: 34  SSTNQTLLFSLKTQKLP----QSSSDKLSFRHNVTLTVTLAVGDPPQNISMVLDTGSELS 93

Query: 98  WLHCNRSQNSSSSSSSTFNPAGSSSYTPIPCSSSTCTDRTRDFPIPASCD-SNHLCHATL 157
           WLHC +S N      S FNP  SS+Y+P+PCSS  C  RTRD PIPASCD   HLCH  +
Sbjct: 94  WLHCKKSPN----LGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPASCDPKTHLCHVAI 153

Query: 158 SYADASSSEGTLATDTFYIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFV 217
           SYADA+S EG LA +TF IG+      +FGCMDS  SSN+EED+K+TGLMGMNRGSLSFV
Sbjct: 154 SYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKSTGLMGMNRGSLSFV 213

Query: 218 SQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEG 277
           +Q+GF KFSYCIS  D SG LLLGDA++SWL P+ YTPL+  STPLPYFDRVAYTVQLEG
Sbjct: 214 NQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEG 273

Query: 278 IKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFE 337
           I+V  K+L +P+SVF PDHTGAGQTMVDSGTQFTFL+GP YTAL++EF+ +T    R+ +
Sbjct: 274 IRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVD 333

Query: 338 DSNFVFQGAMDLCYRV-PMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGE-IRGNDS 397
           D +FVFQG MDLCY+V    +     LP V+L+FRGAEM+V+G ++LYRV G    G + 
Sbjct: 334 DPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGKEE 393

Query: 398 IHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLA-EIRCDLAGQKLGM 449
           ++CFTFGNSDLLG+EAFVIGH HQQNVWMEFDL KSR+G A  +RCDLA Q+LG+
Sbjct: 394 VYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCDLASQRLGL 440

BLAST of Csor.00g094760 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 245.7 bits (626), Expect = 6.9e-65
Identity = 166/449 (36.97%), Postives = 236/449 (52.56%), Query Frame = 0

Query: 17  KSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQV----IPPESIRR---SPDKLP--- 76
           K LF FF     S+  +L   S   P   LP+ T           + R   SP   P   
Sbjct: 6   KPLFFFFFLNYVSLSTSL---SLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNF 65

Query: 77  ---FRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGSSSY 136
              F+++++L +SL +GTPPQ   MV+DTGS+LSW+ C+R +      +S F+P+ SSS+
Sbjct: 66  RSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTS-FDPSLSSSF 125

Query: 137 TPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGIS-N 196
           + +PCS   C  R  DF +P SCDSN LCH +  YAD + +EG L  +     N+ I+  
Sbjct: 126 STLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPP 185

Query: 197 VVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISE------YDFSGL 256
           ++ GC          E S + G++GMNRG LSFVSQ    KFSYCI        +  +G 
Sbjct: 186 LILGCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGS 245

Query: 257 LLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFE 316
             LGD      F +++ L +      S  +P  D +AYTV + GI+   K L I  SVF 
Sbjct: 246 FYLGDNPNSHGFKYVSLLTFPE----SQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFR 305

Query: 317 PDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRV 376
           PD  G+GQTMVDSG++FT L+  AY  +R E + R     +      +V+ G  D+C+  
Sbjct: 306 PDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----KGYVYGGTADMCF-- 365

Query: 377 PMNQTRLPPL-PSVTLVF-RGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA 436
             N   +P L   +  VF RG E+ V  +R+L  V G       IHC   G S +LG  +
Sbjct: 366 DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIHCVGIGRSSMLGAAS 425

Query: 437 FVIGHLHQQNVWMEFDLKKSRIGLAEIRC 440
            +IG++HQQN+W+EFD+   R+G A+  C
Sbjct: 426 NIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

BLAST of Csor.00g094760 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 230.3 bits (586), Expect = 3.0e-60
Identity = 153/457 (33.48%), Postives = 236/457 (51.64%), Query Frame = 0

Query: 17  KSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPES----------IRR--SPDK 76
           K LF   L   F    ++ L  S + +L  PL +  + P +           RR  SP  
Sbjct: 6   KPLFLKLLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPS 65

Query: 77  LPF------RHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN--RSQNSSSSSSSTFN 136
            P+      +++++L +SL +GTP Q+  +V+DTGS+LSW+ C+  + +      +++F+
Sbjct: 66  SPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFD 125

Query: 137 PAGSSSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIG 196
           P+ SSS++ +PCS   C  R  DF +P SCDSN LCH +  YAD + +EG L  + F   
Sbjct: 126 PSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFS 185

Query: 197 NSGIS-NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYD--- 256
           NS  +  ++ GC         +E +   G++GMN G LSF+SQ    KFSYCI       
Sbjct: 186 NSQTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRP 245

Query: 257 ---FSGLLLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLP 316
               +G   LGD      F +++ L +      S  +P  D +AYTV L+GI++  K L 
Sbjct: 246 GLASTGSFYLGDNPNSRGFKYVSLLTF----PQSQRMPNLDPLAYTVPLQGIRIGQKRLN 305

Query: 317 IPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGA 376
           IP SVF PD  G+GQTMVDSG++FT L+  AY  +++E +       +      +V+   
Sbjct: 306 IPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KGYVYGST 365

Query: 377 MDLCYRVPMNQTRLPPLPSVTLVF-RGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSD 436
            D+C+    +      +  +   F RG E+ V    +L  V G       IHC   G S 
Sbjct: 366 ADMCFDGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGIGRSS 425

Query: 437 LLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDL 442
           +LG  + +IG++HQQN+W+EFD+   R+G ++  C L
Sbjct: 426 MLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

BLAST of Csor.00g094760 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-34
Identity = 147/472 (31.14%), Postives = 213/472 (45.13%), Query Frame = 0

Query: 19  LFP--FFLCTLFSVF----QNLILCSSQNPALLLPL--------KTQVIPPE-------S 78
           +FP  FFLC+  S+F     N+   S+ N  L LPL         TQ +  +       S
Sbjct: 1   MFPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLS 60

Query: 79  IRRSPDKLPFRHNVSLT----------VSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNS 138
           +RR P  +PF  +  ++          V L +G PPQ++ ++ DTGS+L W+ C+  +N 
Sbjct: 61  LRRKP--IPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNC 120

Query: 139 SSSSSST-FNPAGSSSYTPIPCSSSTC--TDRTRDFPIPASCDSNHLCHATLSYADASSS 198
           S  S +T F P  SS+++P  C    C    +    PI      +  CH    YAD S +
Sbjct: 121 SHHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLT 180

Query: 199 EGTLATDTFYIGNSG-----ISNVVFGCMDSIFSSNNEEDSKN--TGLMGMNRGSLSFVS 258
            G  A +T  +  S      + +V FGC   I   +    S N   G+MG+ RG +SF S
Sbjct: 181 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFAS 240

Query: 259 QMG---FPKFSYCISEYDFS----GLLLLGDANFSWLAPLNYTPLIEMSTPL-PYFDRVA 318
           Q+G     KFSYC+ +Y  S      L++G+     ++ L +TPL  ++ PL P F    
Sbjct: 241 QLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNGG-DGISKLFFTPL--LTNPLSPTF---- 300

Query: 319 YTVQLEGIKVSHKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNRTA 378
           Y V+L+ + V+   L I  S++E D +G G T+VDSGT   FL  PAY ++        A
Sbjct: 301 YYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSV------IAA 360

Query: 379 GSFRVFEDSNFVFQGAMDLCYRVPMNQTRLPPLPSVTLVFRGAEMTVTGDRILYRVPGEI 438
              RV            DLC  V         LP +   F G  + V   R  +     I
Sbjct: 361 VRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYF-----I 420

Query: 439 RGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAEIRCDL 442
              + I C    + D   V   VIG+L QQ    EFD  +SR+G +   C L
Sbjct: 421 ETEEQIQCLAIQSVD-PKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCAL 451