Csor.00g094300 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g094300
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	Csor_Chr14: 402733 .. 407067 (+)
RNA-Seq Expression	Csor.00g094300
Synteny	Csor.00g094300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACCAGGAGGCCTGTCTTCTCAACACTACCAGAAACTCTTGCTTGATTCTTCAATCATGGGCAAAGCTTCGAGAGATAAGAGGGTTTAAGCACTATTGTCTTTGACTATTTGTCTTTATAATTCATTATCTATTTCCAAGTTTTATTTACTGTGTCTCATTTAGGATATATACTACCGTAAAGCGAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTTGAAGGTTTGTTCTTGTATTGGGCTCCTCTCTATTGAAATATTCTTACATTTCTGCTGGGATGTCAATCACTGAGATGGTCGACATGAATGAGAATAATTATTGCTGCAACATTTTGGATTCCATAACAGAACTATTTTCTAGAAACCTCCTGAAATCTGTGTGATTTATTTTCCTTTGTAATCAACCTGTTTTATCATGTACAAGTCATTTGAAACGCTTCTAAGTTTATCATCTACCATGAAATTTTAAATGCAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTAATTTCTTGGGCGTTTTCTTGCTGCAATTTGTTTATCAAACTAGTGGTCAATGCTTTTTGGGGAATTTTTTTTTCATATAAAACAACTAAATCTAACAAGAATTCTTCAAGTGATAGGCATTGTAAGCATTTTTTGAAAAACATGCCTGCATATGGGGTGCAATACTCTCCTCCCTTCATTGACCCTGCATTTTGGTACACTTTTCAAAAGTAATGATAACATTAAGTATTCTTGAAAAATGGAAGAAAACAACTCGAATTCCTTAATTTTTAAGTACTCTTATAAAAGCTTGTTTTTAAACTTTCATTATCTTATTTGTCAGAACAATATATATTTTTATCTGTATTTTTATATTAACACTCATAGGAGTATTATGCTTCAGTACATTTCACGTTTTGAAAAACACTAAATATAAGGAGGCTGATAAAAGCAATGCAGTATGTGTTGCTGCAACTATTGAACAAAAATATAATTAATTTTGGTTTATTATTTCCAAGTTGTCTTTACATTTTCTCGCCTACTAATCATATGTAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGTAAGTTATGGTAGATTATTGCATGAAAATGTAAATTTTTCCTTCCCATCCTTCTCTCCGTTTTCCCCTTGTTTTCTCCCTTTTCATTGATTCTCTAACTTAACTTGCAGGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCGATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCCGGGTGTACCATGTACGAAGTATTTTTTAACATTTGAAGTGGTAAACGAAGACATATCCAGATCCTAATGCACCACCGACATGGTCAATTTGTTTTAAAATTTCTATTTGTGATAATTTTCCTATGTTTTTCTCAATACTGGATTTTTTTTCTCTTTGAATGAATGCTCACGTTGTGTTAGAAATCTTTTATTTTATTCAACAATTTGATCACATACTAAATTGTCATATGTTTTTGTGCATGGGTTATCTAATATTTAATTTTACCAGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCTGATGGTAGATACTTGTGTTTTTTGAATTCTCTGTTGAGGTTCCACCTGATGATATTTTGTTACTGATTATGTGCTTGTTTGTAGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCTGTAAGTGCTGTGTTTTCTGCAGAGACTGTTTAATTTCTCGAGAAAGTGCTGATGAATATTTTAAGATATTTTGAAAATGCATGGTATATATGCACATTGACAGTAATGATGTTGCTTCATCTCACATTTACTGTGTGGAAAGGATAAAAAACTAAAACCAACAAGAGAAGCACTAATGAGTGTCAAATTATTTGAACCTACAAATAGAAAATAACGTGTAACTAGTGAGCAAAGTAGTTGAGATCCTGTAGGTAAATGGTTGTTGCTGTATTAGGAGGTTATACTGAAGCATTCTAAGGGTTTTTCTATTGTATGTTGCCTGCACTCTTTGATCTTTTTCCTCTATGGACTTAGTTTTGTTGCTTCCATCACTGTCCTCTGATCTGCCTCGTTTTTCCTCCCTCTCTCTCTTCCTTGAGTTAGTTCTCAATATAGTTGGAGATACATAAGGTTTAAGTAACTGAGCTCATCTCGTTTTTTCCCCCTTTTCTTTGCAGGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATTGTCAGGTATATATTAAATGATTGTTATACAATACCCAATTAAAAAAGCATGTAGCATACTCGAGTAACGAAGAAATTTATGGTGTTCTCACTAATGGCATCTACAATATGAATCTCTCTTCTCTTCTACATTTTTTTTCCAGTCAAGTTGGAAAATTTCCTGTGAGCTCTTCTGTTCCTTATAATTAATGCATTGACTTATAGAATTCTTTTAGATATGTTTAGCACTAGTAAAACTTGTCAAGTATTATCATGGATTAAATAAGGATCTTAATCAAAGATTGGTGTTTGTTGTTTCATACATGAAGTGATTGAGCCGTCAATGATGTTTTACATTTTTGGGTTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGGTAACGTTTTGGTTTTCCCACTTTTTTTTCTCCATTGGTAGCTATAGAGGTCTATCTTTCGGTATGATTGAACTTGTTTCCAGAAAATGATACTCCTAATTGAAAATTGCCTTTTTCTCTTTATAGCGTCTGGTTCATAGGATTCTTGCGCATGAATTTTTAGATACCCATTGCACTTGGATTCCTTAAATCAACTTTAGATCGTTATCGTTTAATTCTAGGTGGTGGGACTGGTGTCCACCCAACAGCAAATTGTGGAGTTAGCTCATTTTCGTGGTGACATCTGTAATATAAAAAAGATGTAATAGAGGACAAAATAGTCCAGGGCAAAAGTAAAATTTTTCTAAACTTTCATGGTTGTGTTCGTGAACTAGAAAAAGTTCCAATTACATCCAGCAAGCGTTCTTTTATCTCTTGCTTAAAACAGCAAGTGATTATTTGGCATTATGTACCAGAAGTAGGCATGTTAAACAAAATTATGGAAGATGCGAAAAGACGCGATGGGAGCAATTACATTTTAAACTGAATTTCTGGGTTTGTCTGCCCTTAGTTTAGCTAGTTGGCTTTCTCTTTTATATCACATGTTGGTATATCATATCAAATCATTATTCGTTAGTCTAATCAACGTTCCATGAAAACATTTAAGCCACAATGTTAACCATGGGAAATTAATGGGATCAAAATCAAGAGTTCAAGAACATAATTGGAGCATTTAAAAGTTGAATGGATTAAGTGGATACAATCTTGAACTAAATCTGCGATTCATTCCATTGATTTCTTTTAGTCACCTAGGTGAATCCACGGTAAAGACTTTTAAACAAAGTTATGATTATGATTTAACGTTATGTTCATGTTGAAGCTCATATGTGGATTGGATATGAATGCAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGGTAAACTAATGTACTTCCTTGATTGGGTGTTTACGTATCAACGGATGGACGCATCCTAAGATCAAACATGTTCCTATCCTGTTTTAAAGATTTATTTGTTTTACAGCTGGTGCTATGACCGAGCTGTCTTCTTGTCTTTAGGCATTTTAAAATTAGTGTCCTTGCTGATTTTGATGTGGTCAATTGCCTGGTATTTTTCGTTACACAAGTGGAAGTAATAGCATTGCTATTTTGCTACATTGTGTCCCTCGCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

mRNA sequence

ATGTTACCAGGAGGCCTGTCTTCTCAACACTACCAGAAACTCTTGCTTGATTCTTCAATCATGGGCAAAGCTTCGAGAGATAAGAGGGATATATACTACCGTAAAGCGAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCGATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCTGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

Coding sequence (CDS)

ATGTTACCAGGAGGCCTGTCTTCTCAACACTACCAGAAACTCTTGCTTGATTCTTCAATCATGGGCAAAGCTTCGAGAGATAAGAGGGATATATACTACCGTAAAGCGAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCGATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCTGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

Protein sequence

MLPGGLSSQHYQKLLLDSSIMGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSSDS

Homology

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BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 361.3 bits (926), Expect = 1.2e-98
Identity = 176/300 (58.67%), Postives = 217/300 (72.33%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MG+ S+DKRD+YYR AKE GWRARSAFKLLQ+D EF +F+GV R VDLCAAPGSWSQVLS
Sbjct: 1   MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           +K+          +   H  +VA+DLQ MAP+ GV+Q+QGDIT   TA+ +I+HF GC A
Sbjct: 61  QKI--------GGQGSGH--VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHD+DE++Q+QL+LA L I THVL+ GG F+AKIFRG+D +LLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           ++FF  V  AKP+SSRNSSIEAFAVC+ Y PPEGF P     LL+    P          
Sbjct: 181 QVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ------ 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
             L+GP ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 241 --LDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

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BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 330.5 bits (846), Expect = 2.2e-89
Identity = 174/312 (55.77%), Postives = 214/312 (68.59%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGK SRDKRDIYYR AKE  WRARSAFKL+QIDDEF I +GV+R VDLCAAPGSWSQVLS
Sbjct: 1   MGKTSRDKRDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           ++LY         ++     IVAIDLQPMAPI GVIQ+QGDIT+  TA  VI+HF G K+
Sbjct: 61  KRLY---------EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKS 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           D+V+CDGAPDVTG+H +DEF+Q++LILA   I +HVL+EGG F+AKIFR +++SLLY Q+
Sbjct: 121 DIVICDGAPDVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQM 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           K +F  V  AKP+SSR SS EAF +C +YSPPEGF P              G   LD + 
Sbjct: 181 KKYFKKVYLAKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDATD 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPPIA 320
                P+ +   F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  
Sbjct: 241 ASAISPD-IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTD 290

Query: 321 PPYKRALEMKKA 322
           P YK AL+ KK+
Sbjct: 301 PAYKAALDKKKS 290

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BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.4e-88
Identity = 167/301 (55.48%), Postives = 218/301 (72.43%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MG++S+DKRD YYR AKE+GWRARSAFKLLQ++++FN+FEG KRVVDLCAAPGSWSQVLS
Sbjct: 1   MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLY--LPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGC 140
           R+L   +   ++ D K    P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF   
Sbjct: 61  RELLKNIDTSIAADEK----PMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNE 120

Query: 141 KADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC 200
            ADLVV DGAPDVTGLHD+DE++Q+Q++LA   +   VL+ GGKF+AKIFRG+D SLLY 
Sbjct: 121 PADLVVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYS 180

Query: 201 QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 260
           QL+L F  V+ AKP+SSR SSIE+F VCE+++PP  F P DL + L  V  P+   ++  
Sbjct: 181 QLRLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI-- 240

Query: 261 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 320
                        PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+
Sbjct: 241 ------------APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIEL 279

[Close]

BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.2e-82
Identity = 156/274 (56.93%), Postives = 201/274 (73.36%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGK+S+DKRDIYYRKAKEEGWRARSAFKL+QID+E+ IFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKSSKDKRDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           R++Y   K  PD K      IVA+DLQ MAP++GV+Q++GDIT   T++ +I HFDG  A
Sbjct: 61  RRIYGDGK-DPDVK------IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DL++ DGAPDVTGLHD+D + QSQLIL+ L I TH L+ GG F+AK+F+G D SL+Y Q+
Sbjct: 121 DLIISDGAPDVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQM 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLE---KVGSPSGGDDLD 260
           KLFF  V+F KP SSR SS+E F +C NY PP  +NPK +   LE   K+ + +  +  +
Sbjct: 181 KLFFEHVSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNN 240

Query: 261 CSSGWLEGPN--------KVYIPFLACGDLS-GY 283
            ++  ++  N         + +PF+ACGDL+ GY
Sbjct: 241 NNNCNIDNENDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 159/308 (51.62%), Postives = 212/308 (68.83%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFE--GVKRVVDLCAAPGSWSQV 80
           MGK+S+DKRD+YYRKAKE+G+RARSAFKLLQ++D+F+  +   +KRVVDLCAAPGSWSQV
Sbjct: 1   MGKSSKDKRDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQV 60

Query: 81  LSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGC 140
           LSRKL+     SP S D +   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   
Sbjct: 61  LSRKLF---DESP-SSDKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNE 120

Query: 141 KADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC 200
           KAD V  DGAPDVTGLHD+DE+VQ QLI++ L +   +L++GG F+AKIFRG+D  +LY 
Sbjct: 121 KADFVCSDGAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYS 180

Query: 201 QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK-DLHRLLEKVGSPSGGDDL- 260
           QL   F  +  AKP+SSR +S+EAF VC  Y+PP  + PK D++  +++       + L 
Sbjct: 181 QLGYLFDKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLC 240

Query: 261 --DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQPPIAPPYK 320
             D  S W E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ P  PPYK
Sbjct: 241 ISDKLSHWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYK 300

Query: 321 RALEMKKA 322
           +ALE+K++
Sbjct: 301 KALELKRS 303

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BLAST of Csor.00g094300 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 679 bits (1752), Expect = 2.00e-246
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MLPGGLSSQHYQKLLLDSSIMGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFE 60
           MLPGGLSSQHYQKLLLDSSIMGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFE
Sbjct: 1   MLPGGLSSQHYQKLLLDSSIMGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFE 60

Query: 61  GVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQG 120
           GVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQG
Sbjct: 61  GVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQG 120

Query: 121 DITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREG 180
           DITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREG
Sbjct: 121 DITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREG 180

Query: 181 GKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDL 240
           GKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDL
Sbjct: 181 GKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDL 240

Query: 241 HRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRS 300
           HRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRS
Sbjct: 241 HRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRS 300

Query: 301 LDPVQPPIAPPYKRALEMKKASSQGIRELEKLSSDS 336
           LDPVQPPIAPPYKRALEMKKASSQGIRELEKLSSDS
Sbjct: 301 LDPVQPPIAPPYKRALEMKKASSQGIRELEKLSSDS 336

[Close]

BLAST of Csor.00g094300 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640 bits (1652), Expect = 1.66e-231
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLSSDS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Csor.00g094300 vs. NCBI nr
Match: XP_022983316.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima] >XP_022983317.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 634 bits (1636), Expect = 4.56e-229
Identity = 314/316 (99.37%), Postives = 314/316 (99.37%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLSSDS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Csor.00g094300 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 627 bits (1617), Expect = 3.59e-226
Identity = 310/316 (98.10%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGV+RVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKL+LPAKLSPDSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLHLPAKLSPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Csor.00g094300 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 622 bits (1605), Expect = 2.42e-224
Identity = 308/316 (97.47%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 640 bits (1652), Expect = 8.04e-232
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLSSDS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 2.21e-229
Identity = 314/316 (99.37%), Postives = 314/316 (99.37%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLSSDS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 1.17e-224
Identity = 308/316 (97.47%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 7.91e-223
Identity = 306/316 (96.84%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLSPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL
Match: A0A6J1CWI5 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111015042 PE=3 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 6.50e-222
Identity = 307/316 (97.15%), Postives = 308/316 (97.47%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           RKLYLPAKLS  SKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTA VVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSSVSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKK 300

Query: 321 ASSQGIRELEKLSSDS 336
           ASSQG R LEKLSSDS
Sbjct: 301 ASSQGGRXLEKLSSDS 316

[Close]

BLAST of Csor.00g094300 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-168
Identity = 285/305 (93.44%), Postives = 298/305 (97.70%), Query Frame = 0


Query: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140
           R+LYLPAK S +SKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTH+L+EGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQL 180

Query: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260
           KLFFP VTFAKPKSSRNSSIEAFAVCENYSPPEGFNP+DLHRLLEKVGSPSGG DLDCSS
Sbjct: 181 KLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSS 240

Query: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRALEMK 320
           GWLEGPNKVYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE+K
Sbjct: 241 GWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELK 300

Query: 321 KASSQ 325
           KAS+Q
Sbjct: 301 KASAQ 305

[Close]

BLAST of Csor.00g094300 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 4.9e-31
Identity = 81/240 (33.75%), Postives = 132/240 (55.00%), Query Frame = 0


Query: 21  MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVL 80
           MGK   + + D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W QV 
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 81  SRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDIT----NARTAEVVIRHF 140
             K+ + +            L++ IDL P+ P+ G + +  DIT     ++  +V+ +H 
Sbjct: 61  VEKVPVGS------------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH- 120

Query: 141 DGCKA-DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKD-T 200
            G  A +LV+ DG+P+V G    +   Q+ L++  + + T  L   G  + K+FR +D  
Sbjct: 121 -GVSAFNLVLHDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYN 180

Query: 201 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK--DLHRLLEKVGSPS 252
           S+LYC  +LF  V  F KP +SR++S E + V   Y  P   +P+  D   L ++   P+
Sbjct: 181 SVLYCLGRLFEKVEVF-KPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

[Close]

BLAST of Csor.00g094300 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 7.3e-27
Identity = 56/57 (98.25%), Postives = 57/57 (100.00%), Query Frame = 0


Query: 21 MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQ 78
          MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of Csor.00g094300 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 3.5e-13
Identity = 55/225 (24.44%), Postives = 97/225 (43.11%), Query Frame = 0


Query: 30  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 89
           D +YR+A+  G+ ARSAFKLLQI  ++ + +    V+DL  APG+W QV  +       L
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQ------SL 67

Query: 90  SPDSKDGDHPLIVAIDLQ----PMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVC 149
            P    G   ++V +D++    P      V  +  D+ N    ++           +++ 
Sbjct: 68  GPLRSGG---IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILS 127

Query: 150 DGAPDVTGLHDMDEFVQSQLILAGLTIVT---------------------HVLREGGKFI 209
           D    V+G+   D  + ++L +  L +                        VLR GG  +
Sbjct: 128 DMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGHLV 187

Query: 210 AKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 230
            K+   +D        K  F   ++ +PK++R SS E + +C+ +
Sbjct: 188 IKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of Csor.00g094300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	1.2e-98	58.67	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
Q22031	2.2e-89	55.77	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
O36015	1.4e-88	55.48	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q54VA8	1.2e-82	56.93	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	1.2e-79	51.62	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of Csor.00g094300 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6580486.1	2.00e-246	100.00	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	1.66e-231	100.00	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]
XP_022983316.1	4.56e-229	99.37	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita max...	[more]
XP_038903231.1	3.59e-226	98.10	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]
XP_008442455.1	2.42e-224	97.47	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]

BLAST of Csor.00g094300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1P7	8.04e-232	100.00	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A6J1IYY9	2.21e-229	99.37	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A1S3B6C8	1.17e-224	97.47	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A0A0LCV6	7.91e-223	96.84	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]
A0A6J1CWI5	6.50e-222	97.15	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica c...	[more]

BLAST of Csor.00g094300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	4.2e-168	93.44	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	4.9e-31	33.75	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	7.3e-27	98.25	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	3.5e-13	24.44	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 41..229 e-value: 1.7E-64 score: 217.2
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 40..235 e-value: 1.8E-73 score: 248.5
None	No IPR available	PIRSR	PIRSR005461-1	PIRSR005461-1	coord: 21..231 e-value: 3.0E-67 score: 224.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 317..336
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 320..336
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 21..328
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 21..328
IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7	HAMAP	MF_03162	RNA_methyltr_E_TRM7	coord: 21..288 score: 33.810524
IPR015507	Ribosomal RNA large subunit methyltransferase E	HAMAP	MF_01547	RNA_methyltr_E	coord: 25..230 score: 32.317642
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 40..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g094300.m01	Csor.00g094300.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0002128	tRNA nucleoside ribose methylation
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008175	tRNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity