Csor.00g082020 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g082020
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionFYVE-type domain-containing protein
LocationCsor_Chr09: 409417 .. 413696 (-)
RNA-Seq ExpressionCsor.00g082020
SyntenyCsor.00g082020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAACGGAGATTACGGTACAGCTCCGTATTTTCAATACCAAAATTTTCAAAACCTTACCCTTAATCCTAGCCCTAATCCAGTTCCCATTCCCCCCGATCGCAACCAGAGCGCTTACGCCTCGGCGCCGCCCTTCTCCACCAATTATGCTGCCTCCGATTACCCTGGCTATGCCCCTTATTATCCCCTCTATTCCCAGAATCCCGATCCCCTCCCTGCCTCTCCGACTGCGCCTCTTTTCAACTCTCCCTCATCCAATTCTAATCCCCAGACATTCAATCCCACTCCTCAATCACCCGCTTTCCCTCCATTTGAGTCTCATGTGCCTTATCAATCTCCTTCTCAGTCGCAATCGTATCACTCAACCTACGATCAGCATCAGACGGCTCCCAATTGTGCTCCCCCTCCACCTTCCTCAATTCCCATGAATCAGAGCTCTAATTCTACCCCTACCGCTAATTCTCCGTTCTCATCCATGTATTCGGCTCCCTTTGGCTCTTTGGCACCCCCGTCGTATGAAAATCCATATGAAAGTTCGGTTAAATTTGATCAGGGAGGCGGATATGCTGATGATAGATATGGAGGTTACGGTCGAAGTCGGTCTGATTTTGGTTCGGAGTTGTACGGAAAGCGGCCGGAGGACGCGGCACCGCGCTTCGACTCTGGCTATGATGACGGTTATGGCGATGGAGTCTATGCTTACCAAGGTGGCAAAGTTGAGCCTTATGGTGCTCGTGGTACGGCTTCTAAGTCTTCGACGTGGTCTGCCGCGACGCCTGCTTTTGACGATTATGGGAGGCCGATAAATATTCCTCCGAAAAAAGATCCGCCGGCGAGTTCACTGAAAGTAGTTAGGGCGATCCCTAAGGTGGAAGCTCAAGAAGACGTCAAAAATTGGGTGCAGAAGTTCCGGGTGAAGCTCTTGGCTGAAAGTGGAGGTCAGAGCACCATGGATGTACTTTGTCAGGTATTTCATGCTGTCACAGGACAATTTTGTTGTTGTAGATATTGTCTCGTATTGTGCCTGGTCTGTAGTGGCATGTTTTTTCTCTTCTTAAGGGACAGGCTCTTTAGGCAAACTGCGGAAGATAGGTGTACATGGAATCACGAAGGCGGCTGGTTTTTTATTAAAAAAAAAAAAAAAAAGGAGAAAAACCCCCATGAAAATTGTCATTGTTCTTCTGTTACCTTCTCATCGTTTTTTGTTATCACTTCATATTTTCTTTGCTCCAACATCGTTTAATTGTTATTAAGTTATTTTAGCACAACAATAGGACTTGGTTATTTACTCTCTTTTGTTACCATTTCCAGGTTGGTCTAGATGGCATTCGTATGCTTGATCCAAATTCTAGTCGAACGTTAAGGATATATCCTCTTGAGACCATCACTAGATGCGAAGTAAGTGGGGATGCTAGATTATTTATGGTTTCCTTGGAATTCTCTACCATGGAGTTATCATATGGTTTGTTATCTTTTATTTATGTTTGCAAGGTGTATGATTCTTCCACCCTGGCTTTCTGGTCCAAGAGCTCTGTGGATATTGAGCCACGGCGTATTAGATTACAGTCAAACAAGTACACCACCAACACGCTTCTGGACACGGTGACTGCTGCAACTGTACAGGTTATAATCCCTACCTGTTTTCCTTGGTCTTTAACTTAAGAAAATGATTTGAAGTTTATTTAGTGTTTTCTAACCCTCTTGTTATTTCAAGATACACATTTAACCATTTGCATTAGAAGCATTCTCTTTAGAATTCCTAACCCTGCTAATCTATGCATTTGTGATTTCAAGACATTTTTGAGCTGCAAGGAATTGCCTTTGCCATACCCTTTTGGTATATAAGTGTATTTGCGAATAGTTATCACTTATCATGGTATGATGGATGAGTGTTCTAGTTTATCTTTTAGATCCTTTCGTTCTGTGGCTAAGTCTCAAGGCAACTTCTGTTTAATACCAGTACAGTGTGCACATATCATAGATACACACCGATCAGTCTGTATAACAGTCCAAGCCCACTGCTAGCAAATATTGTCTTCTTTGTGCTTTCCCTTTCGGGCTTCCCCTCAACGTTTTTAAAACACGTCTACTAGGGAGAGGTTCCTACACTCTTATAAAGAATGTTTCGTTCTCCTCCCCAACCAATGTGGAATCTCACAATCCACTCCCCTTTAGGGCCTAGCGTCCTCGTTGGCACTCGTTCCCTTCTCTAATCGATGTGGGATCTCCAATCCACCTCCCTTTCGGGCCCAGCGTTCTTGCTGGCACACCGCCTCGTGTCCACCCCTTTTTGGGGGTTCAGCCTCCTTGTGGCACATCGCCCGGTGTCTGACTCTGATACTAACTGTAACAGCCCAAGCCCACCGCTAGCAGATATTTTCCTCTTTGAACTTTCCCTTTTGGGCATCCCCTCAAGGTTTTCAAAACGTGTTTGCTAGGGAGAGTTTTTCACACTTTTATAAACAATGTTTTGTTCTCCTCCTCAAAACAATGTGAGATCTCACCGTCTGAGTCTTAGATTCTTGATGCACAATAATGTTGACTTCTTTTCATTGAGATTGGGTACTTTTACTGTCTGTTATTAGCTAGTTATTCTAGATATTAGTAGTTGTAACTTGATATGATTCTTTTATTTATTTATTATTATTATTATTCTTATTTTAAAAAACACAGTTTAAGGAAATGGGTGGAAGAAGTAGGCCTTCTGAATCTTTTAAGGCGCCTGAGCAGCCTACAGAGAAGAAGAAAGGGTTGGTTGATTGGGTGAACCTGATAAAGCCAGGCAACGAGGAGAAAGATCATTGGGTATCTTGACAGAGTTGTCATTAATTATCAGCTTGTTGATTTTAAGATTGTCTGAGGGTATATGCATTTACATTGCCTTTTCTGTTTCCGTTTCTAGGTACCTGATGAAGCAGTCACGAAGTGTACAGCCTGTGGGTCAGACTTTGGGGCTTTTGTCCGAAGGGTAAACTTTTTTTTCTAATAATTATAGAATATGGTTCATTAAACTGCCTTTTTTTTTTAATGGATCTCGGTGTTTCAACTTTTTTCTCAGCATCACTGCAGAAATTGTGGAGACATCTTCTGTGATAAATGTACGCAAGGTAGAACAGCTCTGACTGCTGAGGAGAATGCTCCTCAAGTTCGAGTTTGTGACAGATGCATGGTACGTTATGCATAACTTTGATTTATTTGGTATTATTATTATTATTATTAAGAAAAACTTGTATAGAAATTTGTTGATTTGTAATACCATATATCCATTGAATATGTTTCTAAGAAAAACTTCTGTTGAATTTTTTTTTTTCCCTCTTTGACAACTAAAACGTTATTATTATTCTTCCCGCCCGGGCTTATTGGGAAGTGTGGATGATGCGGGTTTATGTATTGGATTAGCTGGTGATCAATTAGCAAGCAGGCATTAGTGTGTTGGCTCTTAACATGTCTTTTCTTGTTTTCAGGCGGAAGTGACTCAGAGGCTCGCTAATGCCAAGGATCTGACTAGTAATCCTGTAGGGTTGCATAGCCATGAGGATCTTGCCAAAAAACTTAAGGTATTGCTTATTGAGCATTGTGATTTCTGTAGTTGAACGCCCTTTCCTAGAGATATTCATCAATAAAAGAAAAAAAATCGTATCTTTCTTATTGCATCAATCTAATTTTAATTTACTGTTCTGTTGTCGTAGGATGAAATGGAGAGAAACCGTAGGTCATCAGGTAATTTGTTGTTCGGGAATCAATAATTTAAATACACTGCTAGCTAGCTGCTCCTCTATTAATATTTGGGGTTGTTATTGTTTAAGTGTTTTTTTTTCTTATATAGGAGAATAAGCACTAATGTTATTGTCACTTCCATGAAATGCCACAGGATCCAAGTCTGATGGATCTGGGAAACGTATGAAGGAAGTTGCATGTCCTACTTGTACTGTGCATTTACAGGTTTGATATCTTCTTTTTTTGGTTTATGATTATAAATTGATTTATTTAGAGTGCTTAGAAAAGTGTTTTGTTAAGGAGTTGGTTATAGTTAGGGGATTCCAAATAATGTTGTATTCAAGTGTGGTGGAGATTGTGATTGCCATGAAATCTTGATCAACACACGGTCCCTTTAAGGCTTGAAGAAAATTAGTTGATGTGGAGCATGTTATGGAGTAGTGCAGTAATGATAAATTTTGTGTGTATACGAACCCATTACAGGTGCAGGTTCCAAGCTCAGGTTCCGAGACCATTGAGTGTGGGGTTTGCCAGCATCCATTCCTTGTCAGCGCTCACTGA

mRNA sequence

ATGCAGAACGGAGATTACGGTACAGCTCCGTATTTTCAATACCAAAATTTTCAAAACCTTACCCTTAATCCTAGCCCTAATCCAGTTCCCATTCCCCCCGATCGCAACCAGAGCGCTTACGCCTCGGCGCCGCCCTTCTCCACCAATTATGCTGCCTCCGATTACCCTGGCTATGCCCCTTATTATCCCCTCTATTCCCAGAATCCCGATCCCCTCCCTGCCTCTCCGACTGCGCCTCTTTTCAACTCTCCCTCATCCAATTCTAATCCCCAGACATTCAATCCCACTCCTCAATCACCCGCTTTCCCTCCATTTGAGTCTCATGTGCCTTATCAATCTCCTTCTCAGTCGCAATCGTATCACTCAACCTACGATCAGCATCAGACGGCTCCCAATTGTGCTCCCCCTCCACCTTCCTCAATTCCCATGAATCAGAGCTCTAATTCTACCCCTACCGCTAATTCTCCGTTCTCATCCATGTATTCGGCTCCCTTTGGCTCTTTGGCACCCCCGTCGTATGAAAATCCATATGAAAGTTCGGTTAAATTTGATCAGGGAGGCGGATATGCTGATGATAGATATGGAGGTTACGGTCGAAGTCGGTCTGATTTTGGTTCGGAGTTGTACGGAAAGCGGCCGGAGGACGCGGCACCGCGCTTCGACTCTGGCTATGATGACGGTTATGGCGATGGAGTCTATGCTTACCAAGGTGGCAAAGTTGAGCCTTATGGTGCTCGTGGTACGGCTTCTAAGTCTTCGACGTGGTCTGCCGCGACGCCTGCTTTTGACGATTATGGGAGGCCGATAAATATTCCTCCGAAAAAAGATCCGCCGGCGAGTTCACTGAAAGTAGTTAGGGCGATCCCTAAGGTGGAAGCTCAAGAAGACGTCAAAAATTGGGTGCAGAAGTTCCGGGTGAAGCTCTTGGCTGAAAGTGGAGGTCAGAGCACCATGGATGTACTTTGTCAGGTTGGTCTAGATGGCATTCGTATGCTTGATCCAAATTCTAGTCGAACGTTAAGGATATATCCTCTTGAGACCATCACTAGATGCGAAGTGTATGATTCTTCCACCCTGGCTTTCTGGTCCAAGAGCTCTGTGGATATTGAGCCACGGCGTATTAGATTACAGTCAAACAAGTACACCACCAACACGCTTCTGGACACGGTGACTGCTGCAACTGTACAGTTTAAGGAAATGGGTGGAAGAAGTAGGCCTTCTGAATCTTTTAAGGCGCCTGAGCAGCCTACAGAGAAGAAGAAAGGGTTGGTTGATTGGGTGAACCTGATAAAGCCAGGCAACGAGGAGAAAGATCATTGGGTACCTGATGAAGCAGTCACGAAGTGTACAGCCTGTGGGTCAGACTTTGGGGCTTTTGTCCGAAGGCATCACTGCAGAAATTGTGGAGACATCTTCTGTGATAAATGTACGCAAGGTAGAACAGCTCTGACTGCTGAGGAGAATGCTCCTCAAGTTCGAGTTTGTGACAGATGCATGGCGGAAGTGACTCAGAGGCTCGCTAATGCCAAGGATCTGACTAGTAATCCTGTAGGGTTGCATAGCCATGAGGATCTTGCCAAAAAACTTAAGGATGAAATGGAGAGAAACCGTAGGTCATCAGGATCCAAGTCTGATGGATCTGGGAAACGTATGAAGGAAGTTGCATGTCCTACTTGTACTGTGCATTTACAGGTGCAGGTTCCAAGCTCAGGTTCCGAGACCATTGAGTGTGGGGTTTGCCAGCATCCATTCCTTGTCAGCGCTCACTGA

Coding sequence (CDS)

ATGCAGAACGGAGATTACGGTACAGCTCCGTATTTTCAATACCAAAATTTTCAAAACCTTACCCTTAATCCTAGCCCTAATCCAGTTCCCATTCCCCCCGATCGCAACCAGAGCGCTTACGCCTCGGCGCCGCCCTTCTCCACCAATTATGCTGCCTCCGATTACCCTGGCTATGCCCCTTATTATCCCCTCTATTCCCAGAATCCCGATCCCCTCCCTGCCTCTCCGACTGCGCCTCTTTTCAACTCTCCCTCATCCAATTCTAATCCCCAGACATTCAATCCCACTCCTCAATCACCCGCTTTCCCTCCATTTGAGTCTCATGTGCCTTATCAATCTCCTTCTCAGTCGCAATCGTATCACTCAACCTACGATCAGCATCAGACGGCTCCCAATTGTGCTCCCCCTCCACCTTCCTCAATTCCCATGAATCAGAGCTCTAATTCTACCCCTACCGCTAATTCTCCGTTCTCATCCATGTATTCGGCTCCCTTTGGCTCTTTGGCACCCCCGTCGTATGAAAATCCATATGAAAGTTCGGTTAAATTTGATCAGGGAGGCGGATATGCTGATGATAGATATGGAGGTTACGGTCGAAGTCGGTCTGATTTTGGTTCGGAGTTGTACGGAAAGCGGCCGGAGGACGCGGCACCGCGCTTCGACTCTGGCTATGATGACGGTTATGGCGATGGAGTCTATGCTTACCAAGGTGGCAAAGTTGAGCCTTATGGTGCTCGTGGTACGGCTTCTAAGTCTTCGACGTGGTCTGCCGCGACGCCTGCTTTTGACGATTATGGGAGGCCGATAAATATTCCTCCGAAAAAAGATCCGCCGGCGAGTTCACTGAAAGTAGTTAGGGCGATCCCTAAGGTGGAAGCTCAAGAAGACGTCAAAAATTGGGTGCAGAAGTTCCGGGTGAAGCTCTTGGCTGAAAGTGGAGGTCAGAGCACCATGGATGTACTTTGTCAGGTTGGTCTAGATGGCATTCGTATGCTTGATCCAAATTCTAGTCGAACGTTAAGGATATATCCTCTTGAGACCATCACTAGATGCGAAGTGTATGATTCTTCCACCCTGGCTTTCTGGTCCAAGAGCTCTGTGGATATTGAGCCACGGCGTATTAGATTACAGTCAAACAAGTACACCACCAACACGCTTCTGGACACGGTGACTGCTGCAACTGTACAGTTTAAGGAAATGGGTGGAAGAAGTAGGCCTTCTGAATCTTTTAAGGCGCCTGAGCAGCCTACAGAGAAGAAGAAAGGGTTGGTTGATTGGGTGAACCTGATAAAGCCAGGCAACGAGGAGAAAGATCATTGGGTACCTGATGAAGCAGTCACGAAGTGTACAGCCTGTGGGTCAGACTTTGGGGCTTTTGTCCGAAGGCATCACTGCAGAAATTGTGGAGACATCTTCTGTGATAAATGTACGCAAGGTAGAACAGCTCTGACTGCTGAGGAGAATGCTCCTCAAGTTCGAGTTTGTGACAGATGCATGGCGGAAGTGACTCAGAGGCTCGCTAATGCCAAGGATCTGACTAGTAATCCTGTAGGGTTGCATAGCCATGAGGATCTTGCCAAAAAACTTAAGGATGAAATGGAGAGAAACCGTAGGTCATCAGGATCCAAGTCTGATGGATCTGGGAAACGTATGAAGGAAGTTGCATGTCCTACTTGTACTGTGCATTTACAGGTGCAGGTTCCAAGCTCAGGTTCCGAGACCATTGAGTGTGGGGTTTGCCAGCATCCATTCCTTGTCAGCGCTCACTGA

Protein sequence

MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAPYYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSYHSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESSVKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKVEPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Homology
BLAST of Csor.00g082020 vs. ExPASy Swiss-Prot
Match: Q9ASS2 (Protein FREE1 OS=Arabidopsis thaliana OX=3702 GN=FREE1 PE=1 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 4.1e-195
Identity = 381/624 (61.06%), Postives = 440/624 (70.51%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPP---------DRNQSAYASAPPFSTNYA 60
           MQ GDY +  + QY  FQN T NP+PNP P PP         D  ++ YASAPPF+  Y 
Sbjct: 1   MQQGDYNSYYHHQYSQFQNPTPNPNPNPNPSPPAPATVAGPTDLTRNTYASAPPFTGGYG 60

Query: 61  ASDYPGYAPYYPLYSQNPDPLPASPTAPLFNSPS--------SNSNP---QTFNPTPQSP 120
           ++DY  Y+  Y  Y QN + +P  P+AP F SPS        ++ NP    TFN  P  P
Sbjct: 61  SADYSNYSQNYTPYGQNSEHVP--PSAPSFTSPSQPPPSPPATSLNPNSYSTFNQPPPPP 120

Query: 121 AFPP--------FESHVPYQSPSQSQSYHSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPT 180
              P        F+S  PYQ P+    Y+S YDQHQT+   + PPPSS P   + N  P 
Sbjct: 121 TIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQTSGYSSAPPPSSAP---APNPNPA 180

Query: 181 ANSPFSSMYSAP----FGSLAPPSYENPYESSVKFDQGGGYADDRYGGYGRSRSDFGSEL 240
             S  SS+YSAP     GS  PPSYE P   SVKFDQ G      Y GY RSRSD GS+L
Sbjct: 181 PYS--SSLYSAPPYSSGGSSIPPSYEKP---SVKFDQSG------YDGYNRSRSDLGSDL 240

Query: 241 YGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKVEPYGARGTASKSSTWSAATPAFDDYGRP 300
           YGKR +         ++D YGDGVYAYQGGKVEPYG+RGTA KSS     +  FDDYGR 
Sbjct: 241 YGKRSDSGE---YPAFEDSYGDGVYAYQGGKVEPYGSRGTAPKSSN----STLFDDYGRS 300

Query: 301 INIPPK---KDPPASSLKVVRAIPKVEAQEDVKNWVQKFRVKLLAESGGQSTMDVLCQVG 360
           I+           ++S K+VRA+PK + QED    VQKFRVKLLAE+ GQ+T DVLCQ+G
Sbjct: 301 ISFSSSGRDSSVSSNSAKIVRAVPKADVQEDSTGGVQKFRVKLLAETYGQTTTDVLCQIG 360

Query: 361 LDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLAFWSKSSVDIEPRRIRLQSNKYTTNT 420
           LDG+RMLDP++SRTLRIYPLE ITRCE  DSS LAFWSK+ VDIE +RIRLQSN YTTNT
Sbjct: 361 LDGLRMLDPSTSRTLRIYPLENITRCEKLDSSILAFWSKTPVDIEAKRIRLQSNSYTTNT 420

Query: 421 LLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEA 480
           LLDTVTAA  Q KE+GG SRP  S K  EQ  EKKKGL DW+N+IKP NEEKDHWVPDEA
Sbjct: 421 LLDTVTAAMFQAKEIGGSSRPPTSGKLIEQTAEKKKGLGDWMNIIKPVNEEKDHWVPDEA 480

Query: 481 VTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTAEENAPQVRVCDRCMAEVTQR 540
           V+KCT+CGSDFGAF+RRHHCRNCGD+FCDKCTQGR ALTAE+NAPQVRVCDRCMAEV+QR
Sbjct: 481 VSKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRIALTAEDNAPQVRVCDRCMAEVSQR 540

Query: 541 LANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSSGSKSDGSGKRMKEVACPTCTVHLQV 590
           L+NAK+ T   V L SHEDLA+KL++EMERNR+SS    +GSG+RMKEVACPTCTVHLQV
Sbjct: 541 LSNAKETTGRNVSLQSHEDLARKLQEEMERNRKSSSGLREGSGRRMKEVACPTCTVHLQV 600

BLAST of Csor.00g082020 vs. ExPASy Swiss-Prot
Match: Q8IZQ1 (WD repeat and FYVE domain-containing protein 3 OS=Homo sapiens OX=9606 GN=WDFY3 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-12
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 0

Query: 426  WVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTA 485
            W    +PG    DHWV DE    C+ C   F    RRHHCRNCG +FC KC++ ++ +  
Sbjct: 3437 WSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKR 3496

Query: 486  EENAPQVRVCDRC 499
             + +  VRVC  C
Sbjct: 3497 LKISSPVRVCQNC 3509

BLAST of Csor.00g082020 vs. ExPASy Swiss-Prot
Match: Q6VNB8 (WD repeat and FYVE domain-containing protein 3 OS=Mus musculus OX=10090 GN=Wdfy3 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-12
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 0

Query: 426  WVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTA 485
            W    +PG    DHWV DE    C+ C   F    RRHHCRNCG +FC KC++ ++ +  
Sbjct: 3419 WSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKR 3478

Query: 486  EENAPQVRVCDRC 499
             + +  VRVC  C
Sbjct: 3479 LKISSPVRVCQNC 3491

BLAST of Csor.00g082020 vs. ExPASy Swiss-Prot
Match: Q54CH1 (Arrestin domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=adcA PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.5e-11
Identity = 31/75 (41.33%), Postives = 38/75 (50.67%), Query Frame = 0

Query: 430 IKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTAEENA 489
           + P    +  W  DE  T C  C   F  F R+HHCR+C  IFCDKCT  +T +T     
Sbjct: 455 LPPDVSYRHPWEGDEHATACRKCNKGFSLFARKHHCRHCMKIFCDKCTSTKTTITKLAYP 514

Query: 490 PQVRVCDRCMAEVTQ 505
             VRVC+ C    TQ
Sbjct: 515 KPVRVCEECYPIATQ 529

BLAST of Csor.00g082020 vs. ExPASy Swiss-Prot
Match: Q15075 (Early endosome antigen 1 OS=Homo sapiens OX=9606 GN=EEA1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 7.3e-11
Identity = 31/63 (49.21%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 440  WVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTAEENAPQVRVCDRCM 499
            W  D  V  C ACG  F   VRRHHCR CG+IFC +C+  + ALT     P VRVCD C 
Sbjct: 1349 WAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECS-AKNALTPSSKKP-VRVCDACF 1408

Query: 500  AEV 503
             ++
Sbjct: 1409 NDL 1409

BLAST of Csor.00g082020 vs. NCBI nr
Match: KAG6591259.1 (Protein FREE1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1192 bits (3083), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY
Sbjct: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of Csor.00g082020 vs. NCBI nr
Match: KAG7024140.1 (Protein FREE1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1189 bits (3076), Expect = 0.0
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY
Sbjct: 61  YYPPYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of Csor.00g082020 vs. NCBI nr
Match: XP_022936845.1 (protein FREE1-like [Cucurbita moschata])

HSP 1 Score: 1186 bits (3067), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLPASPTAPLFNSPSSNSNPQTF PTPQSPAFPPFESHVPYQSPSQSQSY
Sbjct: 61  YYPPYSQNPDPLPASPTAPLFNSPSSNSNPQTFIPTPQSPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of Csor.00g082020 vs. NCBI nr
Match: XP_023535475.1 (protein FREE1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1182 bits (3057), Expect = 0.0
Identity = 584/589 (99.15%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNY ASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYGASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLPASPTAPLFNSPSSNS+PQTFNPTPQSPAFPPFESHVPYQ PSQSQSY
Sbjct: 61  YYPPYSQNPDPLPASPTAPLFNSPSSNSSPQTFNPTPQSPAFPPFESHVPYQPPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQ APNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQAAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of Csor.00g082020 vs. NCBI nr
Match: XP_022975822.1 (protein FREE1-like [Cucurbita maxima])

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 579/589 (98.30%), Postives = 580/589 (98.47%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPI PDRNQSAYASAPPFSTNY ASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPITPDRNQSAYASAPPFSTNYGASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLP SPTAPLFNSPSSNS+PQTFNPTPQSPAFPPFES VPYQS    QSY
Sbjct: 61  YYPPYSQNPDPLPVSPTAPLFNSPSSNSSPQTFNPTPQSPAFPPFESQVPYQS----QSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 585

BLAST of Csor.00g082020 vs. ExPASy TrEMBL
Match: A0A6J1FEV1 (protein FREE1-like OS=Cucurbita moschata OX=3662 GN=LOC111443309 PE=4 SV=1)

HSP 1 Score: 1186 bits (3067), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLPASPTAPLFNSPSSNSNPQTF PTPQSPAFPPFESHVPYQSPSQSQSY
Sbjct: 61  YYPPYSQNPDPLPASPTAPLFNSPSSNSNPQTFIPTPQSPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of Csor.00g082020 vs. ExPASy TrEMBL
Match: A0A6J1IE45 (protein FREE1-like OS=Cucurbita maxima OX=3661 GN=LOC111476412 PE=4 SV=1)

HSP 1 Score: 1165 bits (3014), Expect = 0.0
Identity = 579/589 (98.30%), Postives = 580/589 (98.47%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPI PDRNQSAYASAPPFSTNY ASDYPGYAP
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPITPDRNQSAYASAPPFSTNYGASDYPGYAP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
           YYP YSQNPDPLP SPTAPLFNSPSSNS+PQTFNPTPQSPAFPPFES VPYQS    QSY
Sbjct: 61  YYPPYSQNPDPLPVSPTAPLFNSPSSNSSPQTFNPTPQSPAFPPFESQVPYQS----QSY 120

Query: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180
           HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240
           VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300
           EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420
           FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540
           TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 585

BLAST of Csor.00g082020 vs. ExPASy TrEMBL
Match: A0A5A7V4X1 (Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001250 PE=4 SV=1)

HSP 1 Score: 1103 bits (2852), Expect = 0.0
Identity = 551/593 (92.92%), Postives = 562/593 (94.77%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQY N QNL+LNPSPNPVP P DRN   YASAPPFSTNY ASDYP Y+P
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
            YP YSQNPDP+PASPTAPL+N PSSN N QTFNPTPQ PAFPPFESHVPYQSPSQSQSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAP----PPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENP 180
           +STYDQHQTAPN AP    PPPSSIPMNQSSNSTP+ NSP+SSMYSAPFGSLAPPSYENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 181 YESSVKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240
           YESSVKFDQGGGY DDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 241 GGKVEPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQED 300
           GGK EPYGARGTASKSSTWSAA PAFDDYGRPI+IPPKK+ PASSLKVVRAIPKVEAQED
Sbjct: 241 GGKAEPYGARGTASKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 301 VKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360
            KNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQP 420
           STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRP+ESFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKC 480
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACG+DFGAFVRRHHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERN 540
           TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDL S P GLHSHEDLAKKLKDEMERN
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of Csor.00g082020 vs. ExPASy TrEMBL
Match: A0A5D3E825 (Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007910 PE=4 SV=1)

HSP 1 Score: 1102 bits (2849), Expect = 0.0
Identity = 550/593 (92.75%), Postives = 562/593 (94.77%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQY N QNL+LNPSPNPVP P DRN   YASAPPFSTNY ASDYP Y+P
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNPQTFNPTPQSPAFPPFESHVPYQSPSQSQSY 120
            YP YSQNPDP+PASPTAPL+N PSSN N QTFNPTPQ PAFPPFESHVPYQSPSQSQSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 121 HSTYDQHQTAPNCAP----PPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENP 180
           +STYDQHQTAPN AP    PPPSSIPMNQSSNSTP+ NSP+SSMYSAPFGSLAPPSYENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 181 YESSVKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240
           YESSVKFDQGGGY DDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 241 GGKVEPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQED 300
           GGK EPYGARGT+SKSSTWSAA PAFDDYGRPI+IPPKK+ PASSLKVVRAIPKVEAQED
Sbjct: 241 GGKAEPYGARGTSSKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 301 VKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360
            KNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQP 420
           STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRP+ESFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKC 480
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACG+DFGAFVRRHHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERN 540
           TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDL S P GLHSHEDLAKKLKDEMERN
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of Csor.00g082020 vs. ExPASy TrEMBL
Match: A0A0A0LGJ0 (Zinc finger protein OS=Cucumis sativus OX=3659 GN=Csa_3G873780 PE=4 SV=1)

HSP 1 Score: 1084 bits (2804), Expect = 0.0
Identity = 544/594 (91.58%), Postives = 558/594 (93.94%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60
           MQNGDYGTAPYFQY N QNL+LNPSPNP+P P D N   YASAPPFSTNY ASDYP Y+P
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPLPTPSDSN---YASAPPFSTNYPASDYPAYSP 60

Query: 61  YYPLYSQNPDPLPASPTAPLFNSPSSNSNP--QTFNPTPQSPAFPPFESHVPYQSPSQSQ 120
            YP YSQNPDP+PASPTAPL+N PSSN NP  QTFNPT Q PAFP FESHVPYQSPSQSQ
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNPNPQTFNPTSQPPAFPSFESHVPYQSPSQSQ 120

Query: 121 SYHSTYDQHQTAPNCAP---PPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYEN 180
           SY+STYDQH TAPN AP   PPPSSIPMNQ+SNSTP+ NSP+S MYSAPFGSLAPPSYEN
Sbjct: 121 SYYSTYDQHHTAPNYAPTPTPPPSSIPMNQTSNSTPSPNSPYSPMYSAPFGSLAPPSYEN 180

Query: 181 PYESSVKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAY 240
           PYESSVKFDQGGGY DDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAY
Sbjct: 181 PYESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAY 240

Query: 241 QGGKVEPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQE 300
           QGGKVEPYGARGTASKSSTWSAA PAFDDYGRPI+I PKK+ PASSLKVVRAIPKVEAQE
Sbjct: 241 QGGKVEPYGARGTASKSSTWSAAAPAFDDYGRPISISPKKESPASSLKVVRAIPKVEAQE 300

Query: 301 DVKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYD 360
           D KNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYD
Sbjct: 301 DAKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYD 360

Query: 361 SSTLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQ 420
           SSTLAFWSKSSVDI+PRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRP+ESFKA EQ
Sbjct: 361 SSTLAFWSKSSVDIDPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQ 420

Query: 421 PTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDK 480
            TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACG+DFGAFVRRHHCRNCGDIFCDK
Sbjct: 421 ATEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDK 480

Query: 481 CTQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMER 540
           CTQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDL S P GLHSHEDLAKKLKDEMER
Sbjct: 481 CTQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMER 540

Query: 541 NRRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589
           NRRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 NRRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 591

BLAST of Csor.00g082020 vs. TAIR 10
Match: AT1G20110.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.9e-196
Identity = 381/624 (61.06%), Postives = 440/624 (70.51%), Query Frame = 0

Query: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPP---------DRNQSAYASAPPFSTNYA 60
           MQ GDY +  + QY  FQN T NP+PNP P PP         D  ++ YASAPPF+  Y 
Sbjct: 1   MQQGDYNSYYHHQYSQFQNPTPNPNPNPNPSPPAPATVAGPTDLTRNTYASAPPFTGGYG 60

Query: 61  ASDYPGYAPYYPLYSQNPDPLPASPTAPLFNSPS--------SNSNP---QTFNPTPQSP 120
           ++DY  Y+  Y  Y QN + +P  P+AP F SPS        ++ NP    TFN  P  P
Sbjct: 61  SADYSNYSQNYTPYGQNSEHVP--PSAPSFTSPSQPPPSPPATSLNPNSYSTFNQPPPPP 120

Query: 121 AFPP--------FESHVPYQSPSQSQSYHSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPT 180
              P        F+S  PYQ P+    Y+S YDQHQT+   + PPPSS P   + N  P 
Sbjct: 121 TIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQTSGYSSAPPPSSAP---APNPNPA 180

Query: 181 ANSPFSSMYSAP----FGSLAPPSYENPYESSVKFDQGGGYADDRYGGYGRSRSDFGSEL 240
             S  SS+YSAP     GS  PPSYE P   SVKFDQ G      Y GY RSRSD GS+L
Sbjct: 181 PYS--SSLYSAPPYSSGGSSIPPSYEKP---SVKFDQSG------YDGYNRSRSDLGSDL 240

Query: 241 YGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKVEPYGARGTASKSSTWSAATPAFDDYGRP 300
           YGKR +         ++D YGDGVYAYQGGKVEPYG+RGTA KSS     +  FDDYGR 
Sbjct: 241 YGKRSDSGE---YPAFEDSYGDGVYAYQGGKVEPYGSRGTAPKSSN----STLFDDYGRS 300

Query: 301 INIPPK---KDPPASSLKVVRAIPKVEAQEDVKNWVQKFRVKLLAESGGQSTMDVLCQVG 360
           I+           ++S K+VRA+PK + QED    VQKFRVKLLAE+ GQ+T DVLCQ+G
Sbjct: 301 ISFSSSGRDSSVSSNSAKIVRAVPKADVQEDSTGGVQKFRVKLLAETYGQTTTDVLCQIG 360

Query: 361 LDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLAFWSKSSVDIEPRRIRLQSNKYTTNT 420
           LDG+RMLDP++SRTLRIYPLE ITRCE  DSS LAFWSK+ VDIE +RIRLQSN YTTNT
Sbjct: 361 LDGLRMLDPSTSRTLRIYPLENITRCEKLDSSILAFWSKTPVDIEAKRIRLQSNSYTTNT 420

Query: 421 LLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEA 480
           LLDTVTAA  Q KE+GG SRP  S K  EQ  EKKKGL DW+N+IKP NEEKDHWVPDEA
Sbjct: 421 LLDTVTAAMFQAKEIGGSSRPPTSGKLIEQTAEKKKGLGDWMNIIKPVNEEKDHWVPDEA 480

Query: 481 VTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTALTAEENAPQVRVCDRCMAEVTQR 540
           V+KCT+CGSDFGAF+RRHHCRNCGD+FCDKCTQGR ALTAE+NAPQVRVCDRCMAEV+QR
Sbjct: 481 VSKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRIALTAEDNAPQVRVCDRCMAEVSQR 540

Query: 541 LANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSSGSKSDGSGKRMKEVACPTCTVHLQV 590
           L+NAK+ T   V L SHEDLA+KL++EMERNR+SS    +GSG+RMKEVACPTCTVHLQV
Sbjct: 541 LSNAKETTGRNVSLQSHEDLARKLQEEMERNRKSSSGLREGSGRRMKEVACPTCTVHLQV 600

BLAST of Csor.00g082020 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 4.4e-11
Identity = 31/97 (31.96%), Postives = 49/97 (50.52%), Query Frame = 0

Query: 431 KPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGRTAL------T 490
           +P    +D W+PD++   C  C   F    RRHHCR+CG +FC KCT            T
Sbjct: 27  EPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGKCTANSIPFAPSDLRT 86

Query: 491 AEENAPQVRVCDRCMAEVTQ-----RLANAKDLTSNP 517
             E+  ++RVC+ C  +  Q      ++N  +L+++P
Sbjct: 87  PREDWERIRVCNYCFRQWEQGDGGPHVSNITELSTSP 123

BLAST of Csor.00g082020 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-10
Identity = 36/113 (31.86%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 419 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFC 478
           K  G VD V    P      N  +D W+PD++   C  C + F  F RRHHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 479 DKCTQGRTALTAE------ENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLH 521
            KC        ++      E   ++RVC+ C  +  Q +    D  ++ + LH
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIV-PPDNGASIISLH 118

BLAST of Csor.00g082020 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-10
Identity = 36/113 (31.86%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 419 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFC 478
           K  G VD V    P      N  +D W+PD++   C  C + F  F RRHHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 479 DKCTQGRTALTAE------ENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLH 521
            KC        ++      E   ++RVC+ C  +  Q +    D  ++ + LH
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIV-PPDNGASIISLH 118

BLAST of Csor.00g082020 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-10
Identity = 36/113 (31.86%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 419 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFC 478
           K  G VD V    P      N  +D W+PD++   C  C + F  F RRHHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 479 DKCTQGRTALTAE------ENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLH 521
            KC        ++      E   ++RVC+ C  +  Q +    D  ++ + LH
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIV-PPDNGASIISLH 118

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ASS24.1e-19561.06Protein FREE1 OS=Arabidopsis thaliana OX=3702 GN=FREE1 PE=1 SV=1[more]
Q8IZQ13.0e-1241.10WD repeat and FYVE domain-containing protein 3 OS=Homo sapiens OX=9606 GN=WDFY3 ... [more]
Q6VNB83.0e-1241.10WD repeat and FYVE domain-containing protein 3 OS=Mus musculus OX=10090 GN=Wdfy3... [more]
Q54CH12.5e-1141.33Arrestin domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=adc... [more]
Q150757.3e-1149.21Early endosome antigen 1 OS=Homo sapiens OX=9606 GN=EEA1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
KAG6591259.10.0100.00Protein FREE1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7024140.10.099.83Protein FREE1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022936845.10.099.66protein FREE1-like [Cucurbita moschata][more]
XP_023535475.10.099.15protein FREE1-like [Cucurbita pepo subsp. pepo][more]
XP_022975822.10.098.30protein FREE1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FEV10.099.66protein FREE1-like OS=Cucurbita moschata OX=3662 GN=LOC111443309 PE=4 SV=1[more]
A0A6J1IE450.098.30protein FREE1-like OS=Cucurbita maxima OX=3661 GN=LOC111476412 PE=4 SV=1[more]
A0A5A7V4X10.092.92Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001250... [more]
A0A5D3E8250.092.75Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007910... [more]
A0A0A0LGJ00.091.58Zinc finger protein OS=Cucumis sativus OX=3659 GN=Csa_3G873780 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G20110.12.9e-19661.06RING/FYVE/PHD zinc finger superfamily protein [more]
AT3G14270.14.4e-1131.96phosphatidylinositol-4-phosphate 5-kinase family protein [more]
AT4G33240.12.8e-1031.861-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT4G33240.22.8e-1031.861-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT4G33240.32.8e-1031.861-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 519..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 529..550
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..162
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..195
NoneNo IPR availablePANTHERPTHR46977PROTEIN FREE1coord: 1..589
NoneNo IPR availablePANTHERPTHR46977:SF1PROTEIN FREE1coord: 1..589
NoneNo IPR availableCDDcd15730FYVE_EEA1coord: 440..502
e-value: 2.73033E-23
score: 91.306
IPR000306FYVE zinc fingerSMARTSM00064fyve_4coord: 435..504
e-value: 5.6E-28
score: 108.9
IPR000306FYVE zinc fingerPFAMPF01363FYVEcoord: 439..503
e-value: 6.2E-20
score: 71.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 430..508
e-value: 2.7E-23
score: 83.6
IPR017455Zinc finger, FYVE-relatedPROSITEPS50178ZF_FYVEcoord: 443..503
score: 13.639669
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 438..514

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g082020.m01Csor.00g082020.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070676 intralumenal vesicle formation
biological_process GO:0036258 multivesicular body assembly
cellular_component GO:0000813 ESCRT I complex
cellular_component GO:0031902 late endosome membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043130 ubiquitin binding