Csor.00g066610 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g066610
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRHOMBOID-like protein 12, mitochondrial
LocationCsor_Chr13: 10719564 .. 10722267 (+)
RNA-Seq ExpressionCsor.00g066610
SyntenyCsor.00g066610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAATTCTTTTCTCGCAAACTTGCATCAAGGCTTTCGAATTCCTATGGGAATTCTCACTATTGCAGGGATTGCGCTTCTTTTGCCACACAAGTGCGAACCGGTAACCAAAACTACTTCTATGGCTCCTTCATCACTCGCCCGGTTAAGGATCACTCGCGTTCATGGAGTTCACATCGAAGTTTTACCGAGAAAATTCATGGGTTTTTCTCAGGCCCGGTGGTCGGAAAGCGATTCATATTGAATCCTTCAAACACCCTTTTCAAGGGGTCCATTAAGAGTCTAGTAGGAAGGTCTCTCTTTATGGGAAGCCCATTTTCGAAACTGAAATTTCAGTTGAAACCAGGTTTTGATCATCGATGGCCAGGCAGGTAATGCGTTTGCTCTGTTAGCAACTTCTTTTAATCGCTGAAGAATGTGAAGTCCTTTCATATACAAATGAATTCGTCGCTGAAGAGTGTGAAGTCCTTGTTCTTCATTCAGCGCTGAATATCCTAGTGTCCATAATTTCATTTTCGATGGTGAATTCGAATTTTCCCAATTGCATTGTCCTGTTAAAGTTTGCAAACCATTATGCAATACAATATATTGAATGTTTGCATGAGCCATTGGGAAATCTGATTAGTTCAAGAACAATTGTTATGCTGATTACTACTTGTTGGCATGAGGAGAGTGCGGAACTTCCAACTTCGTCAAATAAGTTCAAGCACTCCAATTTTTAGATGCCCCCCGTAGCATAGAGACTCTAACCTAGATTAGTGCTTGCTGTTTTGAAACTTCCTTCTTTGTTATTTTAGAATTAGCATTGTTCTTATCATGGGTTACTTAAGTTATTGTATAACATATCAAAATAACTTATTAGCCTTCTACATTTGAATAGGCTTGGTGGGTCTTTTAGTATTGAATACAATAAAACAGGTTATAATCATTTGAACAAAGGGGAGGATACTTCCTGATGAACATTGTTGATTATTTGGGGAAGTCCTTATTTTTGTTTTCTTATTCACAGGAGGGCATGGTTTCAAAGGCTGACGACTAATGATGTAGTGTTGGGGTTGATTATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTGTGAGTCCTTGATTGCAATTACATGCCTCAGAATTGTAGGCACCGATAGAATTATTTCTTCTCACCGATTTTGGTTAGGATCCATTTCTCTTGAATTGAGAGACTATTGATGTTCCATGGGAGCTCTTATGGGGCCATTCTTGAGGGAATCAACTATGTCCAGAAATCTAGATTATGAGTAATTGCATGTCTAGGAATCAAGATTAATCGTGTTTGTTTGGCATGATATATTCGTGAAAATTTTGTAATTATGGCTGAAAATATTATTACCTATGTTGCCAATCCAAGGGCACCAATTACCATCCCAAAAGTGATGGTTCAATCTCCCACCCAGCAATTATCGAACAGGAAAAAACTTAGATAACTATGTCTGTTTTGCTAGAATTGTCCATATAAGTGTAATAAAATATCAATTTGTTCTACTGATGCCTCTATAATTTGAAACTAGAGGATTTGGATTAATGATAGGATATCCATCCATTTCATCTCACTCATATCCAAATCACCCCTCCATCGGCATCTCAAACACCAAAGATACTTAGTAAACCACATTCATCGGAAGAGAAACATGGGATTCGACATATCCACCACATGGACGTTTAGAACTTCTCAAGATGAATGGCCCCTGAAAGTTGTTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTCGTTATAAGAGTGTGGAAACCTCTTCTTAGCAGATGCGTTTTAAAACCTTGAGGGGAAGCTTGGAAGGAAACGCCCAAAGAGGATAATATTTCTAGCGGTGAGCTTAGGCTGTTACAAATGGTATTAGAGCTAGACACTGAGCGGTGTGCTAGCGAGGACGCTGAGCCCCGAAGGGGGTGGATTATGAGATCCCACATCAATTGGAGAGAGGAACGAGTGTCAGCGACGACGCTGGGTCTTGAAGGGAGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGGGAACGAAACATTCTTTATGAGGGTGTGGAAACTTCTCCCTAACATATGCGTTTTAAAAACTAAAACTTGAGGGGAAGCCTGGAAGGGAAAGTCCAAAGAGGACAATATCTGTTAGTGGTGGAGCTTGGGCCGTTGCACTTGTGATCAACTAACACCATTAGTAGTTTTTATTTATTTAATTATTTATATATATATATATATATTGTCAAATTGATTAACATGGTTTGAGAAATATTTGTGGCAATGTTACAACATTAGATGCCGTTTTGGAAGTTTTTTAATTTTAAAGCTTGGAACTTCAATTCAACTGTTCTCTGTTGGCAACATTATATTTTCAATATTTCAATTTTCTGTACACAGATCTCTGTAGAAAATGTTAGAAGCGGACGCATACATACATTGATTACATCAGCCTTCAGTCACGTAGAAATTGGCCATATAGTATCCAACATGATTGGACTATACTTCTTTGGGATGAACGTATGGCTATCTTTCTTCGAACTTCTACTAGGTCCTGATTTTATTTGTTTTATTTTCTCTGTTTATATGGGGGTGGCTTGTATAACTTTTTCTTTTGTTATGATCTTTACCGCTTTCCTCGTTTTGGCTTAG

mRNA sequence

ATGAACAAATTCTTTTCTCGCAAACTTGCATCAAGGCTTTCGAATTCCTATGGGAATTCTCACTATTGCAGGGATTGCGCTTCTTTTGCCACACAAGTGCGAACCGGTAACCAAAACTACTTCTATGGCTCCTTCATCACTCGCCCGGTTAAGGATCACTCGCGTTCATGGAGTTCACATCGAAGTTTTACCGAGAAAATTCATGGGTTTTTCTCAGGCCCGGTGGTCGGAAAGCGATTCATATTGAATCCTTCAAACACCCTTTTCAAGGGGTCCATTAAGAGTCTAGTAGGAAGGTCTCTCTTTATGGGAAGCCCATTTTCGAAACTGAAATTTCAGTTGAAACCAGGTTTTGATCATCGATGGCCAGGCAGGAGGGCATGGTTTCAAAGGCTGACGACTAATGATGTAGTGTTGGGGTTGATTATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCTCTGTAGAAAATGTTAGAAGCGGACGCATACATACATTGATTACATCAGCCTTCAGTCACGTAGAAATTGGCCATATAGTATCCAACATGATTGGACTATACTTCTTTGGGATGAACGTATGGCTATCTTTCTTCGAACTTCTACTAGGTCCTGATTTTATTTGTTTTATTTTCTCTGTTTATATGGGGGTGGCTTGTATAACTTTTTCTTTTGTTATGATCTTTACCGCTTTCCTCGTTTTGGCTTAG

Coding sequence (CDS)

ATGAACAAATTCTTTTCTCGCAAACTTGCATCAAGGCTTTCGAATTCCTATGGGAATTCTCACTATTGCAGGGATTGCGCTTCTTTTGCCACACAAGTGCGAACCGGTAACCAAAACTACTTCTATGGCTCCTTCATCACTCGCCCGGTTAAGGATCACTCGCGTTCATGGAGTTCACATCGAAGTTTTACCGAGAAAATTCATGGGTTTTTCTCAGGCCCGGTGGTCGGAAAGCGATTCATATTGAATCCTTCAAACACCCTTTTCAAGGGGTCCATTAAGAGTCTAGTAGGAAGGTCTCTCTTTATGGGAAGCCCATTTTCGAAACTGAAATTTCAGTTGAAACCAGGTTTTGATCATCGATGGCCAGGCAGGAGGGCATGGTTTCAAAGGCTGACGACTAATGATGTAGTGTTGGGGTTGATTATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCTCTGTAGAAAATGTTAGAAGCGGACGCATACATACATTGATTACATCAGCCTTCAGTCACGTAGAAATTGGCCATATAGTATCCAACATGATTGGACTATACTTCTTTGGGATGAACGTATGGCTATCTTTCTTCGAACTTCTACTAGGTCCTGATTTTATTTGTTTTATTTTCTCTGTTTATATGGGGGTGGCTTGTATAACTTTTTCTTTTGTTATGATCTTTACCGCTTTCCTCGTTTTGGCTTAG

Protein sequence

MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSHRSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDHRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFVMIFTAFLVLA
Homology
BLAST of Csor.00g066610 vs. ExPASy Swiss-Prot
Match: Q9FZ81 (RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.9e-29
Identity = 99/247 (40.08%), Postives = 134/247 (54.25%), Query Frame = 0

Query: 6   SRKLASRLSNSYGNSHYCRDCASF---ATQVRTGNQNYFYGSFITRPVK--DHSRSWSSH 65
           S +L   L+N   +SH  R   +      Q R    +Y   S  + PV   D S+ W S 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQTFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWRS- 73

Query: 66  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSK--LKFQLKPGF 125
               EKI GFF+  +  K   L          ++S VG   F+GS F K   +FQ   GF
Sbjct: 74  ----EKIRGFFASALGNKAVKLG-------NLVESRVG---FIGSQFPKKGFEFQRFSGF 133

Query: 126 DHRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHT 185
             R  G + W Q L+  DVVLGL+IAN  VF++WR+ +  FM  NF IS++N +SGR+HT
Sbjct: 134 QRR--GWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLHT 193

Query: 186 LITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFV 245
           LITSAFSH++IGHIVSNMIGLYFFG ++  +F     GP    F+  +Y+  A     F 
Sbjct: 194 LITSAFSHIDIGHIVSNMIGLYFFGTSIARNF-----GPQ---FLLKLYLAGALGGSVFY 235

BLAST of Csor.00g066610 vs. ExPASy Swiss-Prot
Match: Q84WG3 (Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-16
Identity = 61/167 (36.53%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + ++HGFF+G +      L   N         +  R+ F  S      F+     GF 
Sbjct: 52  SRSARVHGFFAGKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 111

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRI--ADPIFMSKNFTISVENVRSGRIH 181
            R  G ++W      N VV GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIH
Sbjct: 112 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIH 171

Query: 182 TLITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIF 225
           TLITS FSHV   HI+ NM+GL +FG  +  SF     GP ++  ++
Sbjct: 172 TLITSGFSHVGATHIILNMMGLCYFGARIARSF-----GPRYLLKLY 201

BLAST of Csor.00g066610 vs. ExPASy Swiss-Prot
Match: B3H707 (Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.9e-13
Identity = 51/147 (34.69%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + K+HGFF+  +      L   N         +  R+ F  S      F+     GF 
Sbjct: 58  SRSAKVHGFFASKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 117

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 181
            R  G ++W      N VV GL+IAN AVF +WR++D     +++ +S  +  SG IHTL
Sbjct: 118 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIHTL 177

Query: 182 ITSAFSHVEIGHIVSNMIGLYFFGMNV 207
           ITS FSH+    I+ NMIG+ +FG  +
Sbjct: 178 ITSGFSHIGTSQIILNMIGISYFGSRI 187

BLAST of Csor.00g066610 vs. NCBI nr
Match: KAG6584400.1 (RHOMBOID-like protein 12, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496 bits (1277), Expect = 5.61e-177
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFVMI 240
           TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFVMI
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFVMI 240

Query: 241 FTAFLVLA 248
           FTAFLVLA
Sbjct: 241 FTAFLVLA 248

BLAST of Csor.00g066610 vs. NCBI nr
Match: KAG7019987.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424 bits (1090), Expect = 3.95e-147
Identity = 209/220 (95.00%), Postives = 212/220 (96.36%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. NCBI nr
Match: XP_022923619.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita moschata])

HSP 1 Score: 419 bits (1076), Expect = 5.34e-145
Identity = 207/220 (94.09%), Postives = 210/220 (95.45%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNK FSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RW GRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. NCBI nr
Match: XP_023519071.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417 bits (1072), Expect = 2.17e-144
Identity = 206/220 (93.64%), Postives = 210/220 (95.45%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNKFFSRKLASRLSNSYGNSHYCRDCASF+TQV TGNQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFSTQVLTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RW GRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. NCBI nr
Match: XP_023001193.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita maxima])

HSP 1 Score: 414 bits (1063), Expect = 5.07e-143
Identity = 205/220 (93.18%), Postives = 209/220 (95.00%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRT NQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RS TEKIHGFFSGPVVGKRFILNPS+TLFKGSIKSLV RSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVERSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. ExPASy TrEMBL
Match: A0A6J1E6X7 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111431274 PE=3 SV=1)

HSP 1 Score: 419 bits (1076), Expect = 2.58e-145
Identity = 207/220 (94.09%), Postives = 210/220 (95.45%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNK FSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RW GRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. ExPASy TrEMBL
Match: A0A6J1KPT4 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495401 PE=3 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 2.46e-143
Identity = 205/220 (93.18%), Postives = 209/220 (95.00%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRT NQNYFYGSFITRPVKDHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLKPGFDH 120
           RS TEKIHGFFSGPVVGKRFILNPS+TLFKGSIKSLV RSLFMGSPFSKLKFQLKPGFDH
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVERSLFMGSPFSKLKFQLKPGFDH 120

Query: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180
           RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI
Sbjct: 121 RWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTLI 180

Query: 181 TSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFI 220
           TSAFSHVEIGHIVSNMIGLYFFGMN+   F     GP+F+
Sbjct: 181 TSAFSHVEIGHIVSNMIGLYFFGMNIGRVF-----GPEFL 215

BLAST of Csor.00g066610 vs. ExPASy TrEMBL
Match: A0A0A0LTI7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 1.08e-118
Identity = 169/207 (81.64%), Postives = 189/207 (91.30%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNK FSRKL SRLSNSY NS  C+DCASF+T VRT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKLFSRKLVSRLSNSYANSLDCKDCASFSTLVRTHNQNHLFGSFVTRPIQDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120
           RSF +KIHGFFS PVVGKRFI+NPSNT FKGS+KS+V  RSLF G+PFSKLKFQLKPGF 
Sbjct: 61  RSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180
           H+W GRR+WFQRLT N+VVLGLIIANVAVFLLWRIADPIFMSKNFTIS+ENVRSGRIHTL
Sbjct: 121 HQWGGRRSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLENVRSGRIHTL 180

Query: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNV 206
           ITSAFSH++ GHIVSNM+GLYFFGMN+
Sbjct: 181 ITSAFSHIDTGHIVSNMVGLYFFGMNI 207

BLAST of Csor.00g066610 vs. ExPASy TrEMBL
Match: A0A5D3BLA0 (RHOMBOID-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001490 PE=3 SV=1)

HSP 1 Score: 348 bits (894), Expect = 1.25e-117
Identity = 168/207 (81.16%), Postives = 188/207 (90.82%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNK FSRKLASRLSNSY NS YC+DCASF+T  RT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120
           RSF +KIHGFFS PVVGKR ++NPSNT FKGS+KS+V  RSLF G+PFSKLKFQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180
           H+W GRR+WFQRLT  DVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNV 206
           ITSAFSH++ GH++SNMIGLYFFGMN+
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNI 207

BLAST of Csor.00g066610 vs. ExPASy TrEMBL
Match: A0A1S3BSX2 (RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 PE=3 SV=1)

HSP 1 Score: 348 bits (894), Expect = 1.25e-117
Identity = 168/207 (81.16%), Postives = 188/207 (90.82%), Query Frame = 0

Query: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60
           MNK FSRKLASRLSNSY NS YC+DCASF+T  RT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120
           RSF +KIHGFFS PVVGKR ++NPSNT FKGS+KS+V  RSLF G+PFSKLKFQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180
           H+W GRR+WFQRLT  DVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNV 206
           ITSAFSH++ GH++SNMIGLYFFGMN+
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNI 207

BLAST of Csor.00g066610 vs. TAIR 10
Match: AT1G18600.1 (RHOMBOID-like protein 12 )

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-30
Identity = 99/247 (40.08%), Postives = 134/247 (54.25%), Query Frame = 0

Query: 6   SRKLASRLSNSYGNSHYCRDCASF---ATQVRTGNQNYFYGSFITRPVK--DHSRSWSSH 65
           S +L   L+N   +SH  R   +      Q R    +Y   S  + PV   D S+ W S 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQTFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWRS- 73

Query: 66  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSK--LKFQLKPGF 125
               EKI GFF+  +  K   L          ++S VG   F+GS F K   +FQ   GF
Sbjct: 74  ----EKIRGFFASALGNKAVKLG-------NLVESRVG---FIGSQFPKKGFEFQRFSGF 133

Query: 126 DHRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHT 185
             R  G + W Q L+  DVVLGL+IAN  VF++WR+ +  FM  NF IS++N +SGR+HT
Sbjct: 134 QRR--GWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLHT 193

Query: 186 LITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIFSVYMGVACITFSFV 245
           LITSAFSH++IGHIVSNMIGLYFFG ++  +F     GP    F+  +Y+  A     F 
Sbjct: 194 LITSAFSHIDIGHIVSNMIGLYFFGTSIARNF-----GPQ---FLLKLYLAGALGGSVFY 235

BLAST of Csor.00g066610 vs. TAIR 10
Match: AT1G74130.2 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-17
Identity = 61/167 (36.53%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + ++HGFF+G +      L   N         +  R+ F  S      F+     GF 
Sbjct: 52  SRSARVHGFFAGKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 111

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRI--ADPIFMSKNFTISVENVRSGRIH 181
            R  G ++W      N VV GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIH
Sbjct: 112 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIH 171

Query: 182 TLITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIF 225
           TLITS FSHV   HI+ NM+GL +FG  +  SF     GP ++  ++
Sbjct: 172 TLITSGFSHVGATHIILNMMGLCYFGARIARSF-----GPRYLLKLY 201

BLAST of Csor.00g066610 vs. TAIR 10
Match: AT1G74130.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-17
Identity = 61/167 (36.53%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + ++HGFF+G +      L   N         +  R+ F  S      F+     GF 
Sbjct: 52  SRSARVHGFFAGKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 111

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRI--ADPIFMSKNFTISVENVRSGRIH 181
            R  G ++W      N VV GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIH
Sbjct: 112 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIH 171

Query: 182 TLITSAFSHVEIGHIVSNMIGLYFFGMNVWLSFFELLLGPDFICFIF 225
           TLITS FSHV   HI+ NM+GL +FG  +  SF     GP ++  ++
Sbjct: 172 TLITSGFSHVGATHIILNMMGLCYFGARIARSF-----GPRYLLKLY 201

BLAST of Csor.00g066610 vs. TAIR 10
Match: AT1G74140.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-14
Identity = 51/147 (34.69%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + K+HGFF+  +      L   N         +  R+ F  S      F+     GF 
Sbjct: 58  SRSAKVHGFFASKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 117

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 181
            R  G ++W      N VV GL+IAN AVF +WR++D     +++ +S  +  SG IHTL
Sbjct: 118 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIHTL 177

Query: 182 ITSAFSHVEIGHIVSNMIGLYFFGMNV 207
           ITS FSH+    I+ NMIG+ +FG  +
Sbjct: 178 ITSGFSHIGTSQIILNMIGISYFGSRI 187

BLAST of Csor.00g066610 vs. TAIR 10
Match: AT1G74140.4 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-14
Identity = 51/147 (34.69%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 62  SFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRSLFMGSPFSKLKFQLK--PGFD 121
           S + K+HGFF+  +      L   N         +  R+ F  S      F+     GF 
Sbjct: 58  SRSAKVHGFFASKLGNTNLKLKFGNV--------MESRAGFFSSELPSHGFESGGFTGFQ 117

Query: 122 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 181
            R  G ++W      N VV GL+IAN AVF +WR++D     +++ +S  +  SG IHTL
Sbjct: 118 KR--GWKSWIN--GANGVVFGLVIANAAVFTMWRVSD-----RSWMLSTYSFTSGYIHTL 177

Query: 182 ITSAFSHVEIGHIVSNMIGLYFFGMNV 207
           ITS FSH+    I+ NMIG+ +FG  +
Sbjct: 178 ITSGFSHIGTSQIILNMIGISYFGSRI 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ811.9e-2940.08RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12... [more]
Q84WG31.9e-1636.53Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16... [more]
B3H7071.9e-1334.69Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17... [more]
Match NameE-valueIdentityDescription
KAG6584400.15.61e-177100.00RHOMBOID-like protein 12, mitochondrial, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7019987.13.95e-14795.00RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022923619.15.34e-14594.09RHOMBOID-like protein 12, mitochondrial [Cucurbita moschata][more]
XP_023519071.12.17e-14493.64RHOMBOID-like protein 12, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_023001193.15.07e-14393.18RHOMBOID-like protein 12, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E6X72.58e-14594.09RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KPT42.46e-14393.18RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A0A0LTI71.08e-11881.64Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE... [more]
A0A5D3BLA01.25e-11781.16RHOMBOID-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BSX21.25e-11781.16RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 ... [more]
Match NameE-valueIdentityDescription
AT1G18600.11.4e-3040.08RHOMBOID-like protein 12 [more]
AT1G74130.21.3e-1736.53Rhomboid-related intramembrane serine protease family protein [more]
AT1G74130.11.3e-1736.53Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.11.4e-1434.69Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.41.4e-1434.69Rhomboid-related intramembrane serine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 131..246
e-value: 1.1E-14
score: 56.4
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 134..210
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 172..233
e-value: 3.7E-6
score: 27.0
NoneNo IPR availablePANTHERPTHR43731RHOMBOID PROTEASEcoord: 17..240
NoneNo IPR availablePANTHERPTHR43731:SF22RHOMBOID-LIKE PROTEIN 12, MITOCHONDRIALcoord: 17..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g066610.m01Csor.00g066610.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity