Csor.00g063720 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g063720
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	High-affinity nitrate transporter
Location	Csor_Chr13: 9206802 .. 9207823 (+)
RNA-Seq Expression	Csor.00g063720
Synteny	Csor.00g063720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGCTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGGTAAGATTTTTCAGCTCCACCTTTAGATATCTTGTAACATTTCCCTAATACACACAAAGCCCGAAAGGAAAGCTAAAAAGGAGAATATTTACATTGATGAGTTCGTGCCGTCACAAATAGTATTAGAGTCTTGACACTAGATGATCTGTCAATGAGAAGGCTGAGCCCCGAGGAAGATGGACACAAGACCGAAGGGAGATGGATAGGAGGTGGTGTGGCAACAAGGATGCTAGACTCCAAAAGGGTGGATTTGGCATGGATTGTGAAAATTCGAGAGGGAAAGCATATCCACTAGCTATGTGCTTGATCGATCTGAATCCAAAATTGGAACTTTACCCATTTCTAACAACTGGGTTGTCTTTTTTTTTCTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACCGGTCGCCATATCTCACTTGACATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGAACAGTAA

mRNA sequence

ATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGCTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACCGGTCGCCATATCTCACTTGACATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGAACAGTAA

Coding sequence (CDS)

ATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGCTCTGTTTCTTGGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCACCTAAACCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGTCAAGTGGACCGGGCCTGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGGGAACAAGACGGTACAAAAGTTCGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACGAACTTGTTTCAAATCGAAGCGATCACCGGTCGCCATATCTCACTTGACATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTAGCCGGCAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGAACAGTAA

Protein sequence

MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ

Homology

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BLAST of Csor.00g063720 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-49
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHI 186
            +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g063720 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 8.8e-47
Identity = 100/207 (48.31%), Postives = 136/207 (65.70%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPRSLEVTASP-KPGQVLKAGVDKISVTW 66
           M+ +  L  +L +   +  S G      F+ L  S+EVTA P K   VL+AG D +++TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 67  VL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGN 126
            L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y   +
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 120

Query: 127 KTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISL 186
           KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF + AI+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 187 DIASVCFSAFSVLSLSGFFLAGKRKAK 209
           DIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of Csor.00g063720 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-41
Identity = 84/180 (46.67%), Postives = 121/180 (67.22%), Query Frame = 0


Query: 30  IHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVS 89
           +  S LP++L V  SPK G+V+ AG + ++VTW LN +  +G+D+++K+V+ KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 90  QVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNS 149
           + DR WRK  DDL KDK CQF +  +PY  G     +F++ VARDIPT ++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 150 AGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKK 209
           +G EV YGQ++ +      F +  ITG H SL +A+  FS FS+ +L+ FF+  KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]

BLAST of Csor.00g063720 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.8e-39
Identity = 93/206 (45.15%), Postives = 123/206 (59.71%), Query Frame = 0


Query: 14  LAALFLLCFLGFSH-------GHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 73
           L  L LL  LG  H            S+L ++L V ASP+ GQVL AG D I+VTW LN 
Sbjct: 13  LPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNA 72

Query: 74  T---VKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKT 133
           T     +G+D+ YK V+  LCYAP SQV R WRK  DDLSKDK CQF I  +PY+     
Sbjct: 73  TAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAG-- 132

Query: 134 VQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDI 193
             KFE+TVARD+PT +++VRAYA +++G  V YG+T  +      F +  ITG   S+++
Sbjct: 133 --KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTASIEV 192

Query: 194 ASVCFSAFSVLSLSGFFLAGKRKAKK 210
           A+   SAFSV +L+ F +   +K  K
Sbjct: 193 AAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]

BLAST of Csor.00g063720 vs. NCBI nr
Match: KAG6584111.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019702.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431 bits (1109), Expect = 1.82e-152
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG
Sbjct: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215

[Close]

BLAST of Csor.00g063720 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 426 bits (1095), Expect = 2.48e-150
Identity = 212/215 (98.60%), Postives = 213/215 (99.07%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFL CFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG
Sbjct: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAG+EVGYGQTTDNKKGTNLFQIEAITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAP Q
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of Csor.00g063720 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422 bits (1084), Expect = 1.18e-148
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFL CF GFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLFCFFGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 
Sbjct: 61  TWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPA 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIE+ITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAP Q
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of Csor.00g063720 vs. NCBI nr
Match: XP_023001304.1 (high-affinity nitrate transporter 3.1-like [Cucurbita maxima])

HSP 1 Score: 420 bits (1080), Expect = 4.81e-148
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFL CFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 
Sbjct: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPA 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQT+DNKKGTNLFQI+AITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRK KKAEAP Q
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKTKKAEAPGQ 215

[Close]

BLAST of Csor.00g063720 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 355 bits (911), Expect = 2.57e-122
Identity = 174/206 (84.47%), Postives = 187/206 (90.78%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++NGFLLAALFL CFLGFS G+IH SSLPR+LEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV KPYNP NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212
           CFSAFSV+SL GFF   KRKAKKA A
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAA 206

[Close]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 426 bits (1095), Expect = 1.20e-150
Identity = 212/215 (98.60%), Postives = 213/215 (99.07%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFL CFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG
Sbjct: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAG+EVGYGQTTDNKKGTNLFQIEAITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAP Q
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 2.33e-148
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 1   MEGSINMSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60
           MEGSINMSANGFLLAALFL CFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV
Sbjct: 1   MEGSINMSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISV 60

Query: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPG 120
           TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 
Sbjct: 61  TWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPA 120

Query: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHIS 180
           NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQT+DNKKGTNLFQI+AITGRHIS
Sbjct: 121 NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHIS 180

Query: 181 LDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215
           LDIASVCFSAFSVLSLSGFFLAGKRK KKAEAP Q
Sbjct: 181 LDIASVCFSAFSVLSLSGFFLAGKRKTKKAEAPGQ 215

[Close]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 355 bits (911), Expect = 1.25e-122
Identity = 174/206 (84.47%), Postives = 187/206 (90.78%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++NGFLLAALFL CFLGFS G+IH SSLPR+LEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV KPYNP NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212
           CFSAFSV+SL GFF   KRKAKKA A
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAA 206

[Close]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 355 bits (911), Expect = 1.25e-122
Identity = 174/206 (84.47%), Postives = 187/206 (90.78%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++NGFLLAALFL CFLGFS G+IH SSLPR+LEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV KPYNP NKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212
           CFSAFSV+SL GFF   KRKAKKA A
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAA 206

[Close]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 354 bits (909), Expect = 2.34e-122
Identity = 176/206 (85.44%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66
           M++N FLLAALFL CFLGFSHG+IHFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126
           TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF IV K YNP NKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186
           FEWTV RDIPTGTFFVRAY  NSAGEEV YGQTTD KKGTNLFQIE+ITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212
           CFSAFSV+SL GFF   KRKAKKA A
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAA 206

[Close]

BLAST of Csor.00g063720 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHI 186
            +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g063720 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHI 186
            +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g063720 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 106/209 (50.72%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPR-SLEVTASPK---PGQVLKAGVDKIS 66
           M+    L A+L +   +   HG      F  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 67  VTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNP 126
           +TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 127 GNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHI 186
            +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNLF ++AI+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 187 SLDIASVCFSAFSVLSLSGFFLAGKRKAK 209
           SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Csor.00g063720 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 6.2e-48
Identity = 100/207 (48.31%), Postives = 136/207 (65.70%), Query Frame = 0


Query: 7   MSANGFLLAALFLLCFLGFSHGHIH---FSSLPRSLEVTASP-KPGQVLKAGVDKISVTW 66
           M+ +  L  +L +   +  S G      F+ L  S+EVTA P K   VL+AG D +++TW
Sbjct: 23  MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 82

Query: 67  VL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGN 126
            L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y   +
Sbjct: 83  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 142

Query: 127 KTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISL 186
           KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF + AI+G H+ L
Sbjct: 143 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 202

Query: 187 DIASVCFSAFSVLSLSGFFLAGKRKAK 209
           DIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 203 DIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of Csor.00g063720 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 4.5e-46
Identity = 94/184 (51.09%), Postives = 125/184 (67.93%), Query Frame = 0


Query: 26  SHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVL-NGTVKSGSDSSYKTVEAKLC 85
           SHG +H  SL  +LE         VL+AG D +++TW L + + K  +D+++KT++ KLC
Sbjct: 265 SHG-VHHRSLEAALECP------PVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 86  YAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRA 145
           YAP+SQVDR WRKT++ L KD++C   IV K Y   +KT Q  +WT+  DIPTGT+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRA 384

Query: 146 YAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGK 205
           Y  +  G EV YGQ+TD  + TNLF + AI+G H+ LDIAS  FS FSV+SL  FF+  K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 206 RKAK 209
           RKAK
Sbjct: 445 RKAK 438

The following BLAST results are available for this feature:

BLAST of Csor.00g063720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGS5	1.4e-49	50.72	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q9SB67	8.8e-47	48.31	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q6ZI50	1.4e-41	46.67	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
Q7XK12	1.8e-39	45.15	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of Csor.00g063720 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6584111.1	1.82e-152	100.00	High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022924070.1	2.48e-150	98.60	high-affinity nitrate transporter 3.1 [Cucurbita moschata]	[more]
XP_023520111.1	1.18e-148	97.21	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023001304.1	4.81e-148	97.21	high-affinity nitrate transporter 3.1-like [Cucurbita maxima]	[more]
XP_008439011.1	2.57e-122	84.47	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266...	[more]

BLAST of Csor.00g063720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EDS1	1.20e-150	98.60	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A6J1KMC9	2.33e-148	97.21	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE...	[more]
A0A5A7ULV7	1.25e-122	84.47	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3AXC1	1.25e-122	84.47	High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S...	[more]
C8CHG8	2.34e-122	85.44	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2	[more]

BLAST of Csor.00g063720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50200.3	1.0e-50	50.72	nitrate transmembrane transporters	[more]
AT5G50200.2	1.0e-50	50.72	nitrate transmembrane transporters	[more]
AT5G50200.1	1.0e-50	50.72	nitrate transmembrane transporters	[more]
AT4G24730.4	6.2e-48	48.31	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT4G24730.1	4.5e-46	51.09	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 7..211 e-value: 3.0E-81 score: 269.4
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 32..207 e-value: 1.3E-73 score: 246.1
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 11..210
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 11..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g063720.m01	Csor.00g063720.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity