Csor.00g061520 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g061520
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionalkaline ceramidase
LocationCsor_Chr03: 5695754 .. 5696095 (+)
RNA-Seq ExpressionCsor.00g061520
SyntenyCsor.00g061520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATACTGGCCATTGGTAGTATGTTCTTCCATGCCACATTGCAAAAAGTTTCGTTCCTCTTCCCCTATGGAGTCATGTTCGCCGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGAATCTTTGCCTCCTCTGCGTACCTCGGATGTACAAGTATTATATGTGCACACAAGATACCTCGGCTAAGCGGTTAGCAAAGCTGTACTCGTCGACGTTTCTCTTAGGCATTTTCTGTTGGGTCATCGATCGGGGTACTGCAAGAAGATATCAGATTGGATCATCAATCCTTAAGGTCATGCCTTGTGGCATGTTTTCATGGGTTTGA

mRNA sequence

ATGATACTGGCCATTGGTAGTATGTTCTTCCATGCCACATTGCAAAAAGTTTCGTTCCTCTTCCCCTATGGAGTCATGTTCGCCGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGAATCTTTGCCTCCTCTGCGTACCTCGGATGTACAAGTATTATATGTGCACACAAGATACCTCGGCTAAGCGGTTAGCAAAGCTGTACTCGTCGACGTTTCTCTTAGGCATTTTCTGTTGGGTCATCGATCGGGGTACTGCAAGAAGATATCAGATTGGATCATCAATCCTTAAGGTCATGCCTTGTGGCATGTTTTCATGGGTTTGA

Coding sequence (CDS)

ATGATACTGGCCATTGGTAGTATGTTCTTCCATGCCACATTGCAAAAAGTTTCGTTCCTCTTCCCCTATGGAGTCATGTTCGCCGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGAATCTTTGCCTCCTCTGCGTACCTCGGATGTACAAGTATTATATGTGCACACAAGATACCTCGGCTAAGCGGTTAGCAAAGCTGTACTCGTCGACGTTTCTCTTAGGCATTTTCTGTTGGGTCATCGATCGGGGTACTGCAAGAAGATATCAGATTGGATCATCAATCCTTAAGGTCATGCCTTGTGGCATGTTTTCATGGGTTTGA

Protein sequence

MILAIGSMFFHATLQKVSFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILKVMPCGMFSWV
Homology
BLAST of Csor.00g061520 vs. ExPASy Swiss-Prot
Match: Q94IB9 (Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.2e-24
Identity = 60/121 (49.59%), Postives = 72/121 (59.50%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSM +HATLQ V                                +FLF YG  FA
Sbjct: 69  MILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFLFLYGAAFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 90
           + H+ LR+ IGFKVHYV LCLLC+PRMYKYY+ T+DT+AKR+AK Y +T L+G  CW  D
Sbjct: 129 IVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILVGSICWFCD 188

BLAST of Csor.00g061520 vs. NCBI nr
Match: KAG6603812.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 235 bits (600), Expect = 3.43e-78
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKVSFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM 60
           MILAIGSMFFHATLQKVSFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM
Sbjct: 1   MILAIGSMFFHATLQKVSFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM 60

Query: 61  CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILKVMPCGMFSWV 113
           CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILKVMPCGMFSWV
Sbjct: 61  CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILKVMPCGMFSWV 113

BLAST of Csor.00g061520 vs. NCBI nr
Match: KAG7033992.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 209 bits (531), Expect = 1.94e-67
Identity = 102/103 (99.03%), Postives = 102/103 (99.03%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKVSFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM 60
           MILAIGSMFFHATLQKV FLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM
Sbjct: 1   MILAIGSMFFHATLQKVWFLFPYGVMFAVAHSILRYDIGFKVHYVNLCLLCVPRMYKYYM 60

Query: 61  CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILK 103
           CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILK
Sbjct: 61  CTQDTSAKRLAKLYSSTFLLGIFCWVIDRGTARRYQIGSSILK 103

BLAST of Csor.00g061520 vs. NCBI nr
Match: XP_008440061.1 (PREDICTED: alkaline ceramidase 3 [Cucumis melo] >XP_008440062.1 PREDICTED: alkaline ceramidase 3 [Cucumis melo])

HSP 1 Score: 139 bits (351), Expect = 1.88e-38
Identity = 75/126 (59.52%), Postives = 82/126 (65.08%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG MFA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 94
           VAHSILRYDIGFKVHYV LCLLC+PRMYKYY+ T+D SAKRLA+LYS T LLG FCWV D
Sbjct: 129 VAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSD 188

BLAST of Csor.00g061520 vs. NCBI nr
Match: XP_038882449.1 (alkaline ceramidase [Benincasa hispida] >XP_038882450.1 alkaline ceramidase [Benincasa hispida])

HSP 1 Score: 139 bits (351), Expect = 1.88e-38
Identity = 75/126 (59.52%), Postives = 84/126 (66.67%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG MFA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 94
           VAHSILRYDIGFKVHYV LCLLC+PRMYKYY+ T+D SAKRLA+LYS T LLGIFCWV+D
Sbjct: 129 VAHSILRYDIGFKVHYVILCLLCMPRMYKYYIYTKDASAKRLARLYSLTLLLGIFCWVMD 188

BLAST of Csor.00g061520 vs. NCBI nr
Match: XP_023003815.1 (alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucurbita maxima] >XP_023003817.1 alkaline ceramidase [Cucurbita maxima] >XP_023003819.1 alkaline ceramidase [Cucurbita maxima] >XP_023003820.1 alkaline ceramidase [Cucurbita maxima])

HSP 1 Score: 139 bits (349), Expect = 3.76e-38
Identity = 78/144 (54.17%), Postives = 88/144 (61.11%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG +FA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 112
           VAHSILRYDIGFK+HYV LCLLC+PRMYKYY+ T+D SAKRLA+LY  T LLGIFCWVID
Sbjct: 129 VAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLLGIFCWVID 188

BLAST of Csor.00g061520 vs. ExPASy TrEMBL
Match: A0A1S3B0Z4 (alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1)

HSP 1 Score: 139 bits (351), Expect = 9.10e-39
Identity = 75/126 (59.52%), Postives = 82/126 (65.08%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG MFA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 94
           VAHSILRYDIGFKVHYV LCLLC+PRMYKYY+ T+D SAKRLA+LYS T LLG FCWV D
Sbjct: 129 VAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSD 188

BLAST of Csor.00g061520 vs. ExPASy TrEMBL
Match: A0A6J1KSV2 (alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1)

HSP 1 Score: 139 bits (349), Expect = 1.82e-38
Identity = 78/144 (54.17%), Postives = 88/144 (61.11%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG +FA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 112
           VAHSILRYDIGFK+HYV LCLLC+PRMYKYY+ T+D SAKRLA+LY  T LLGIFCWVID
Sbjct: 129 VAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLLGIFCWVID 188

BLAST of Csor.00g061520 vs. ExPASy TrEMBL
Match: A0A6J1EME1 (alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1)

HSP 1 Score: 136 bits (342), Expect = 2.05e-37
Identity = 77/144 (53.47%), Postives = 87/144 (60.42%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQKV                                +FLF YG +FA
Sbjct: 69  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 112
           VAHSILRYDIGFK+HYV LCLLC+PRMYKYY+ T+D SAKRLA+LY  T LLGIFCWVID
Sbjct: 129 VAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLLGIFCWVID 188

BLAST of Csor.00g061520 vs. ExPASy TrEMBL
Match: A0A6J1BUA9 (alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1)

HSP 1 Score: 132 bits (332), Expect = 6.54e-36
Identity = 75/144 (52.08%), Postives = 85/144 (59.03%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSMF+HATLQK+                                +FLF YG +FA
Sbjct: 69  MILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 112
           VAHSILRY IGFKVHYV LCLLC+PRMYKYY+ T+D SAKRLA+LY  T  LGI CWVID
Sbjct: 129 VAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFLGILCWVID 188

BLAST of Csor.00g061520 vs. ExPASy TrEMBL
Match: A0A7J0FYQ4 (Alkaline phytoceramidase OS=Actinidia rufa OX=165716 GN=Acr_15g0019080 PE=3 SV=1)

HSP 1 Score: 129 bits (324), Expect = 3.33e-35
Identity = 72/142 (50.70%), Postives = 87/142 (61.27%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSM FHATLQ+V                                +FLF YG +FA
Sbjct: 69  MILAIGSMLFHATLQQVQQQSDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGALFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFC-WVI 109
           VAHS +R+ IGFKVHY  LC LC+PRMYKYY+ TQD SAKR+AKLY++T  LG  C   +
Sbjct: 129 VAHSQIRFSIGFKVHYAILCFLCIPRMYKYYIHTQDVSAKRIAKLYAATLFLGSVCVGCL 188

BLAST of Csor.00g061520 vs. TAIR 10
Match: AT4G22330.1 (Alkaline phytoceramidase (aPHC) )

HSP 1 Score: 112.1 bits (279), Expect = 3.0e-25
Identity = 60/121 (49.59%), Postives = 72/121 (59.50%), Query Frame = 0

Query: 1   MILAIGSMFFHATLQKV--------------------------------SFLFPYGVMFA 60
           MILAIGSM +HATLQ V                                +FLF YG  FA
Sbjct: 69  MILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFLFLYGAAFA 128

Query: 61  VAHSILRYDIGFKVHYVNLCLLCVPRMYKYYMCTQDTSAKRLAKLYSSTFLLGIFCWVID 90
           + H+ LR+ IGFKVHYV LCLLC+PRMYKYY+ T+DT+AKR+AK Y +T L+G  CW  D
Sbjct: 129 IVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILVGSICWFCD 188

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94IB94.2e-2449.59Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6603812.13.43e-78100.00Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7033992.11.94e-6799.03Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008440061.11.88e-3859.52PREDICTED: alkaline ceramidase 3 [Cucumis melo] >XP_008440062.1 PREDICTED: alkal... [more]
XP_038882449.11.88e-3859.52alkaline ceramidase [Benincasa hispida] >XP_038882450.1 alkaline ceramidase [Ben... [more]
XP_023003815.13.76e-3854.17alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucu... [more]
Match NameE-valueIdentityDescription
A0A1S3B0Z49.10e-3959.52alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1[more]
A0A6J1KSV21.82e-3854.17alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1[more]
A0A6J1EME12.05e-3753.47alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1[more]
A0A6J1BUA96.54e-3652.08alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1[more]
A0A7J0FYQ43.33e-3550.70Alkaline phytoceramidase OS=Actinidia rufa OX=165716 GN=Acr_15g0019080 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G22330.13.0e-2549.59Alkaline phytoceramidase (aPHC) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044219Alkaline ceramidase, plantPANTHERPTHR46852ALKALINE CERAMIDASEcoord: 18..96

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g061520.m01Csor.00g061520.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006914 autophagy
biological_process GO:0006672 ceramide metabolic process
biological_process GO:0098542 defense response to other organism
biological_process GO:0009651 response to salt stress
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides