Csor.00g061280 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g061280
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	DNA oxidative demethylase ALKBH2
Location	Csor_Chr03: 5784955 .. 5786116 (+)
RNA-Seq Expression	Csor.00g061280
Synteny	Csor.00g061280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTTGAGGTTCAAAGAAGTGATCGACTCAGAATCGGAGACGAAGATCCCTCGTCCTTTAGATTTAGGGAGGCGACAGACATTGGATCTCGGAAACGGAAGCGAAATCGTCTATATTAGCAAGTTCGTGTCCTCTGATCAGGCTTGGACCTGGTTCGATTTTCTCAATCAACACATTCCATGGACTAGACCGACGATTCGCGTCTTCGGTCGCTCCGTCGTCCAGGTCTTTACTTCTCTCGATTATGTTCTCTCGTTTAGTTCATTGTCAACCTAATTAGCAATTGAAGATTTAACGACTGGCTGTTGGTTGTCTTAGATGATTTTCCTTCTTTCCCGCCATTCTTTATATCGGGATTTTGCGTTCTTTCTGAAGAGCTATTGCATTTTCTAATATGCAGCCTAGAGATACTTGTTACGTTGTGAATCCTGGATTGACGACGTATAGTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTCCTCCTCTCAAGGACATTTTGAATGCTGTAAGATATTTTGATTCAATCTTTCCCTATTGGGTTTTCTTTTTGGCTTCTTTAGCTACAGGTTCTCGATGACGTTTCTAGTGGTTCCTAATTGTCTTCACTTTTTCTCTGTCAAGGTTCACGAAGCTCTACCGGGGAGTAGTTTTAATAGCTTACTCTTGAATAGATATAAAGGTGGAAATGATTATGTGGGTTGGCATTCTGACGATGAAAAGCTTTATGCATTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAATAAAACTTCTCAACGTATGCGGTCTAGTTCATCTGTTTTTCTCGTCTTTGTTCTGATTTTGAAAAGCTCTTTTGTCAAATCTTCAGTCTTTTGCTCAATTGATTTCTTCACTCCAAAAAGTTTCATTATCTTATGGTTTATTTGATGTGGCCCCAGGAAGGAACGATGAAGAACCTCCAGCCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCTTTTGTTCTAAAGCATGGTTCGCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTTGTTGA

mRNA sequence

ATGAGTTTGAGGTTCAAAGAAGTGATCGACTCAGAATCGGAGACGAAGATCCCTCGTCCTTTAGATTTAGGGAGGCGACAGACATTGGATCTCGGAAACGGAAGCGAAATCGTCTATATTAGCAAGTTCGTGTCCTCTGATCAGGCTTGGACCTGGTTCGATTTTCTCAATCAACACATTCCATGGACTAGACCGACGATTCGCGTCTTCGGTCGCTCCGTCGTCCAGCCTAGAGATACTTGTTACGTTGTGAATCCTGGATTGACGACGTATAGTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTCCTCCTCTCAAGGACATTTTGAATGCTGTTCACGAAGCTCTACCGGGGAGTAGTTTTAATAGCTTACTCTTGAATAGATATAAAGGTGGAAATGATTATGTGGGTTGGCATTCTGACGATGAAAAGCTTTATGCATTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAATAAAACTTCTCAACGAAGGAACGATGAAGAACCTCCAGCCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCTTTTGTTCTAAAGCATGGTTCGCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTTGTTGA

Coding sequence (CDS)

ATGAGTTTGAGGTTCAAAGAAGTGATCGACTCAGAATCGGAGACGAAGATCCCTCGTCCTTTAGATTTAGGGAGGCGACAGACATTGGATCTCGGAAACGGAAGCGAAATCGTCTATATTAGCAAGTTCGTGTCCTCTGATCAGGCTTGGACCTGGTTCGATTTTCTCAATCAACACATTCCATGGACTAGACCGACGATTCGCGTCTTCGGTCGCTCCGTCGTCCAGCCTAGAGATACTTGTTACGTTGTGAATCCTGGATTGACGACGTATAGTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTCCTCCTCTCAAGGACATTTTGAATGCTGTTCACGAAGCTCTACCGGGGAGTAGTTTTAATAGCTTACTCTTGAATAGATATAAAGGTGGAAATGATTATGTGGGTTGGCATTCTGACGATGAAAAGCTTTATGCATTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAATAAAACTTCTCAACGAAGGAACGATGAAGAACCTCCAGCCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCTTTTGTTCTAAAGCATGGTTCGCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTTGTTGA

Protein sequence

MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVC

Homology

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BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 317.8 bits (813), Expect = 1.1e-85
Identity = 150/243 (61.73%), Postives = 187/243 (76.95%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSL     ++S S +  P       R+T+DLG+GS+++YI +F+   Q+WT+FD+L++HI
Sbjct: 77  MSLTSIVAVESPSSSNAP------SRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHI 136

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRS +QPRDTCYV + GLT   YSGY+P +Y+WDDFPPLK+IL+A+++ L
Sbjct: 137 PWTRPTIRVFGRSCLQPRDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVL 196

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQ-- 180
           PGS FNSLLLNRYKG +DYV WH+DDEK+Y    EIASVSFGCERDF+LKKK ++ S   
Sbjct: 197 PGSRFNSLLLNRYKGASDYVAWHADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQG 256

Query: 181 RRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTF 240
           +  D  P  K+ K+SS  D  S  LKHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTF
Sbjct: 257 KTGDSGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTF 313

Query: 241 RHV 242
           R V
Sbjct: 317 RLV 313

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BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot
Match: Q96Q83 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606 GN=ALKBH3 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.4e-22
Identity = 74/211 (35.07%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 35  SEIVYISKFVSSDQAWTWFDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYS 94
           S +     FV   +A    + L Q +PW + T      +  QPR T +    G   Y+YS
Sbjct: 88  SRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLY 154
                  PH   W   P L+ + N + E   G +FNSLL N Y+   D V WHSDDE   
Sbjct: 148 RITMEPNPH---W--HPVLRTLKNRIEEN-TGHTFNSLLCNLYRNEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLV 214
                IAS+SFG  R F ++KK            PP +++   +  +     L HG+LL+
Sbjct: 208 GRCPIIASLSFGATRTFEMRKK------------PPPEENGDYTYVERVKIPLDHGTLLI 267

Query: 215 MKGYTQRDWMHSVPKRLKAEATRINLTFRHV 242
           M+G TQ DW H VPK   +   R+NLTFR V
Sbjct: 268 MEGATQADWQHRVPKEYHSREPRVNLTFRTV 277

[Close]

BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot
Match: Q32L00 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 8.4e-22
Identity = 76/211 (36.02%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 35  SEIVYISKFVSSDQAWTWFDFLNQHIPWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYS 94
           S +     FV   +A + F+ L + +PW + T      +  QPR T +    G   Y+YS
Sbjct: 88  SRVCLCPGFVDLKEADSVFEQLCRDVPWKQRTGIRDDVTYQQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLY 154
                  PH   W   P L  + N + E   G SFNSLL N Y+   D V WHSDDE   
Sbjct: 148 RITMEPNPH---W--HPVLLMLKNQIEEN-TGHSFNSLLCNLYRNEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLV 214
                IAS+SFG  R F ++KK            PP + +   +  +     L HG+LL+
Sbjct: 208 GRCPIIASLSFGATRMFEMRKK------------PPPEDNGDYTYVERVKIPLDHGTLLI 267

Query: 215 MKGYTQRDWMHSVPKRLKAEATRINLTFRHV 242
           M+G TQ DW H VPK   +   RINLTFR V
Sbjct: 268 MEGATQADWQHRVPKEYHSREPRINLTFRTV 277

[Close]

BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot
Match: Q58DM4 (DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.4e-21
Identity = 65/175 (37.14%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 67  IRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFN 126
           ++VFG+    PR      + GL TY++SG       W   P L+ + + V   + G +FN
Sbjct: 98  VQVFGKWHSVPRKQATYGDTGL-TYTFSGLTLSPKPW--IPVLERVRDRV-SLVTGQTFN 157

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  AL   IASVSFG  RDF+ + K ++          P
Sbjct: 158 FVLINRYKDGQDHIGEHRDDERELALGSPIASVSFGACRDFVFRHKDSRGKH-------P 217

Query: 187 AKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHV 242
           +++       +     L HGSLL+M   T   W HS+P R K  A R+NLTFR +
Sbjct: 218 SRR------LEVVRLQLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKI 255

[Close]

BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot
Match: Q6P6J4 (DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.1e-20
Identity = 64/175 (36.57%), Postives = 91/175 (52.00%), Query Frame = 0


Query: 67  IRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFN 126
           ++VFG+    PR      + GL TY++SG       W   P L+ + + V E + G +FN
Sbjct: 77  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLTPKPW--VPVLERVRDRVCE-VTGQTFN 136

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++  + R      
Sbjct: 137 FVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFIFRHKDSRGKRPRR----- 196

Query: 187 AKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHV 242
                     +     L HGSLL+M   T   W HS+P R +  A R+NLTFR +
Sbjct: 197 --------TVEVVRLQLAHGSLLMMNPPTNTHWYHSLPIRKRVLAPRVNLTFRKI 234

[Close]

BLAST of Csor.00g061280 vs. NCBI nr
Match: KAG6603836.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 502 bits (1292), Expect = 1.82e-179
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 VC 242
           VC
Sbjct: 241 VC 242

[Close]

BLAST of Csor.00g061280 vs. NCBI nr
Match: XP_022950477.1 (DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 DNA oxidative demethylase ALKBH2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498 bits (1283), Expect = 2.15e-177
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 254

[Close]

BLAST of Csor.00g061280 vs. NCBI nr
Match: XP_022950476.1 (DNA oxidative demethylase ALKBH2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 494 bits (1271), Expect = 2.69e-175
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 239
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of Csor.00g061280 vs. NCBI nr
Match: XP_022977708.1 (DNA oxidative demethylase ALKBH2 [Cucurbita maxima])

HSP 1 Score: 491 bits (1265), Expect = 3.54e-174
Identity = 238/239 (99.58%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLT YSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTIYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 239
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of Csor.00g061280 vs. NCBI nr
Match: XP_004134811.1 (DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742940.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >KGN49032.1 hypothetical protein Csa_003044 [Cucumis sativus])

HSP 1 Score: 467 bits (1202), Expect = 1.03e-165
Identity = 225/241 (93.36%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEV DSESETKIPRP DLG+RQTLDLGNGSEI+YISKFVSSDQAWTWFDFLNQ I
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTI VFGRSV+QPRDTCYV NPGLTT +YSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKSSVAD HSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

[Close]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 1.04e-177
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 254

[Close]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL
Match: A0A6J1GEX8 (DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 494 bits (1271), Expect = 1.30e-175
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 239
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL
Match: A0A6J1IQU8 (DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=4 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 1.71e-174
Identity = 238/239 (99.58%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYVVNPGLT YSYSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTIYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 239
           NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL
Match: A0A0A0KL20 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510990 PE=4 SV=1)

HSP 1 Score: 467 bits (1202), Expect = 5.00e-166
Identity = 225/241 (93.36%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEV DSESETKIPRP DLG+RQTLDLGNGSEI+YISKFVSSDQAWTWFDFLNQ I
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTI VFGRSV+QPRDTCYV NPGLTT +YSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKSSVAD HSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

[Close]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL
Match: A0A5D3CPV3 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00320 PE=4 SV=1)

HSP 1 Score: 458 bits (1179), Expect = 1.60e-162
Identity = 219/241 (90.87%), Postives = 229/241 (95.02%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSLRFKEV DSE+ETKIPRP D G+RQTLDLGNGSEI+YISKFVSSDQAWTWFD+LNQ I
Sbjct: 1   MSLRFKEVTDSETETKIPRPFDPGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSV+QPRDTCYV +PG TT +YSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGSTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKK S ADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

[Close]

BLAST of Csor.00g061280 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 317.8 bits (813), Expect = 7.7e-87
Identity = 150/243 (61.73%), Postives = 187/243 (76.95%), Query Frame = 0


Query: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60
           MSL     ++S S +  P       R+T+DLG+GS+++YI +F+   Q+WT+FD+L++HI
Sbjct: 77  MSLTSIVAVESPSSSNAP------SRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHI 136

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRS +QPRDTCYV + GLT   YSGY+P +Y+WDDFPPLK+IL+A+++ L
Sbjct: 137 PWTRPTIRVFGRSCLQPRDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVL 196

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQ-- 180
           PGS FNSLLLNRYKG +DYV WH+DDEK+Y    EIASVSFGCERDF+LKKK ++ S   
Sbjct: 197 PGSRFNSLLLNRYKGASDYVAWHADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQG 256

Query: 181 RRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTF 240
           +  D  P  K+ K+SS  D  S  LKHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTF
Sbjct: 257 KTGDSGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTF 313

Query: 241 RHV 242
           R V
Sbjct: 317 RLV 313

The following BLAST results are available for this feature:

BLAST of Csor.00g061280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	1.1e-85	61.73	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]
Q96Q83	6.4e-22	35.07	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606...	[more]
Q32L00	8.4e-22	36.02	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 G...	[more]
Q58DM4	2.4e-21	37.14	DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1	[more]
Q6P6J4	2.1e-20	36.57	DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1	[more]

BLAST of Csor.00g061280 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603836.1	1.82e-179	100.00	DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022950477.1	2.15e-177	100.00	DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 D...	[more]
XP_022950476.1	2.69e-175	100.00	DNA oxidative demethylase ALKBH2 isoform X1 [Cucurbita moschata]	[more]
XP_022977708.1	3.54e-174	99.58	DNA oxidative demethylase ALKBH2 [Cucurbita maxima]	[more]
XP_004134811.1	1.03e-165	93.36	DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative...	[more]

BLAST of Csor.00g061280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEX9	1.04e-177	100.00	DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1GEX8	1.30e-175	100.00	DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IQU8	1.71e-174	99.58	DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=...	[more]
A0A0A0KL20	5.00e-166	93.36	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A5D3CPV3	1.60e-162	90.87	DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Csor.00g061280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22260.1	7.7e-87	61.73	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 26..242 e-value: 3.2E-61 score: 208.7
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 37..239 e-value: 1.2E-34 score: 120.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..196
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..195
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..241
IPR032852	DNA oxidative demethylase ALKBH2	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 24..241
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 124..242 score: 12.916455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g061280.m01	Csor.00g061280.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0032259	methylation
biological_process	GO:0035511	oxidative DNA demethylation
biological_process	GO:0006281	DNA repair
molecular_function	GO:0051747	cytosine C-5 DNA demethylase activity
molecular_function	GO:0043734	DNA-N1-methyladenine dioxygenase activity
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0008168	methyltransferase activity