Csor.00g058670 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g058670
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Cysteine desulfurase
Location	Csor_Chr03: 6976182 .. 6978883 (+)
RNA-Seq Expression	Csor.00g058670
Synteny	Csor.00g058670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGGCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCACTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCGGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATGTGAGCCTGAAGAGTATTGGGGCTCTGAATCTAACAAATGCATTCTTAAGAAGGTTAACGATTTTATTTAAGTTCTTAATCTTTGTCTCTTTACTTTTTGTTCTACCTGATTCATCAAAGGACAATTTTTTAGCTAAATGCCTTTTATTTTCTTCTCCCTTCTTTTTGGTTGAATAGGTATGAATTTATGGTTTCTCTTCCTTTTTTACTGTTATATAGCAGATTACATTTCATTCACAAGTTTTTTTTTGTAAGGCGCACAACTTTTTATTAAAGACGATGAGACATCCTCTCGTCTCACTCCCAGCTGACTAAGATTACAGAAATTTCTTAATCAAGAATACAAGAATGGTTATGAAATCATCCTTTAGACAAAGGAATAGCCTTCAATTGGCTTAGGTTTCATAGATATGATGTTCAGATTAATTTTAGACTCATGGTTCCACTTCTACCAATGCAAGATGAATGGGTGTGATGGTACCCGCCTTAAGTAGATGAATAGTTTAGTCTCATGGAGCAACGTTTATTGGGGTAAACGGCTAGTGAAAATCCTTATAGTTTTTAAGACCATGACACCTAGACAGTTATACAATTCTCATTCTTAACCTGATACGTCTGGACCGTGAAGTTTTTGTATGAACAGCAACTTTATGCAAGTAAAATGGCCAGTGCAGTTTTGTCGCCATCTACAGTGACACTCTGGATCAAGAAGACCCTGGCTATACCACTATTAATAGTGCCTCTTCTAGATTAGGACTCTCTTTATTTATGTAAGCACTTGCTTCCATAAGGTTTTTCTTTCATTCAACTTCAACTTCTTGCAGTTGCAGACCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGGATGCTAGCCTTGCTGCAGGTAGACTGTATTAAGCCTTTACTGTAGTTTTTTAGGATAAATCAGTTGAAAATTCTGTGCACGGTCATTGACAGCTATGATGATCTATTTTATAAAATTAACATTCTAAATCTTCCTATAAAAAAACTAACATCCTAAATCCACGTCTTCAGCTGTTTAAAATGCAGTGAAACTTTCTAGGTATAAGACCAATAAGGAGATTCTAAAGAGAACTCTTTCAGTAGATCATTTTTTTTAAAAAAAGAATTATTTATTTATTCATTTGTTGCAGTATTGTTGGTTGTCATGAATCCACACGTGTTAGGATATACATCTATTTCTACAATTATGTGTTCTAGAATGAACATCCTAAGTCCATGTCTTCTGATTTCCAAAATGAAGTGGAACTTTGTTGGATTTATTCCTTCTGGGTTTGCTATTGCCTGAAAGTTTGTTCATGCAAAGGTGCAACCTTTTCTTGTTGGTCTGACTTTCTCACCAAGGAAAAGGTTCTTTGCCCTGTGGAACTTGAAACAGTGAAGCGTTCTATGGTCAAGGGGCTAGCTACTCTGCAGGAAGCCCCCCACCCCTCCCCAAAAAAAAAAAAAATGGAAGACATCCTGGCTTCTAGGCGTGCTGTAGCGAATATGAATGGGTGTGGTAAATTGAAATTTCAGATTTCCATGCGGATAAGAGCAAGAAGTGATTGAATGGCCATGTCTATGAAGTTCAACCTACTTGCCCAGTTGAGACCGTGTAGGAGCCGAGGAGGCGCAGTTTTCTTATTGGACTTTCACTGTTTGGTTGTACACAAATGAGGACAATAAAATAAGTGAATGTTTTAGTGTTCCGCTAACAGTTTGCTAACACCAATACAGTTGTCTTGGTACTGCGTTCTTTTTTATGTTTATAAGTGAAGATGTCAACAAAACCTCTTTATTCACGAGCAGTGTACAACTTATTCTGTACAGCATCAAATTTACTCCGACTTGGAACTTTATGCATCCTTGATACTATTTTATTTTCTTCTACGAAATGGAAGTTTTCATTTAGATATGAAAACTAGTACCTCTGATAGCAATATCATCATGAGCCAGCAGGACCCTGGAGACCTTGAAACAAGCTGTGAAAATCTTCTTGCTCCAGACAGAATCAATACATAATAGAAAGTTAGAAGAAACCAGTGGGTGGGGGTTTGAAATGGTGGGTGGGTGTGTGTGCTTGGTCTTTAATTGAGACTGCATTATTGTTTGAAACATGCAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCAGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCCATGTGCAGTGCAGTGGCCAAGTTTTGGGCAATCTGACTGA

mRNA sequence

ATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGGCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCACTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCGGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATGTGAGCCTGAAGAGTATTGGGGCTCTGAATCTAACAAATGCATTCTTAAGAAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCAGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCCATGTGCAGTGCAGTGGCCAAGTTTTGGGCAATCTGACTGA

Coding sequence (CDS)

ATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGGCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCACTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCGGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATGTGAGCCTGAAGAGTATTGGGGCTCTGAATCTAACAAATGCATTCTTAAGAAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCAGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCCATGTGCAGTGCAGTGGCCAAGTTTTGGGCAATCTGACTGA

Protein sequence

MVGNRIMPSSALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNVSLKSIGALNLTNAFLRRYCVAISIKCPCLQRRKQLLKRRGGSAEEAPCAVQWPSFGQSD

Homology

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BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.1e-61
Identity = 108/145 (74.48%), Postives = 131/145 (90.34%), Query Frame = 0


Query: 12  LIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGF 71
           LI ASPKEIVF SGATE+NN+++KG M+FY++ K+H+ITTQTEHKCVLDSCRHLQQ  GF
Sbjct: 109 LIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQE-GF 168

Query: 72  DITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHT 131
           ++TYLPV +DG+VDLE LR  IRPDTGLVS+MAVNNEIGV+QP+EEIG ICKE NVPFHT
Sbjct: 169 EVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHT 228

Query: 132 DAAQALGEIPIDLEKWNVSLKSIGA 157
           DAAQA+G+IP+D++KWNV+L S+ A
Sbjct: 229 DAAQAIGKIPVDVKKWNVALMSMSA 252

[Close]

BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot
Match: P0DN31 (Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05732-2 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-51
Identity = 97/152 (63.82%), Postives = 121/152 (79.61%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYRE--KKRHLITTQTEHKCVLDSCRHLQQ 69
           + LIGA PKEI+FTSGATESNN+SIKG   F+    KK+H+ITTQTEHKCVLDSCRHLQ 
Sbjct: 156 ATLIGADPKEIIFTSGATESNNMSIKGVARFFGRSGKKKHIITTQTEHKCVLDSCRHLQD 215

Query: 70  RVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNV 129
             GF++TYLPV S+G++ +E L + IRP+T LVS+M VNNEIGVIQP++EIG +C+   V
Sbjct: 216 E-GFEVTYLPVQSNGLIKIEDLEAAIRPETALVSIMTVNNEIGVIQPMKEIGALCRSKKV 275

Query: 130 PFHTDAAQALGEIPIDLEKWNVSLKSIGALNL 160
            FHTDAAQA+G+IP+D+ +WNV L SI    L
Sbjct: 276 FFHTDAAQAVGKIPVDVNEWNVDLMSISGHKL 306

[Close]

BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot
Match: Q54X04 (Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=nfs1 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-51
Identity = 96/145 (66.21%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           S +IGA  KEI+FTSGATES NI+IKG   FY+EKK H+ITT TEHKC+LDSCRHL+   
Sbjct: 104 SKIIGADSKEIIFTSGATESGNIAIKGVARFYKEKKNHIITTVTEHKCILDSCRHLEME- 163

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF +TYLPVG +G+VDLE L++ I P T LV++MAVNNEIGV+QP++EIGKIC+E  V F
Sbjct: 164 GFKVTYLPVGENGLVDLELLKNTITPQTSLVTIMAVNNEIGVVQPIKEIGKICRENGVFF 223

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSI 155
           HTDAAQA+G+IPID+   N+ L SI
Sbjct: 224 HTDAAQAVGKIPIDVNDMNIDLLSI 247

[Close]

BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot
Match: P87187 (Cysteine desulfurase, mitochondrial OS=Candida maltosa OX=5479 GN=SPL1 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-50
Identity = 95/145 (65.52%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 12  LIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGF 71
           +I A PKEI+FTSGATE+NN++IKG   FY++ K+H+ITTQTEHKCVLDS RH+Q   GF
Sbjct: 139 VINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDE-GF 198

Query: 72  DITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHT 131
           DITYLPV S+G+++L+ L+  IR DT LVSVMAVNNEIGVIQPL+EIG+IC+E  + FHT
Sbjct: 199 DITYLPVNSEGLINLDDLKKAIRKDTVLVSVMAVNNEIGVIQPLKEIGQICRENKIFFHT 258

Query: 132 DAAQALGEIPIDLEKWNVSLKSIGA 157
           DAAQA G+IPID+ + N+ L SI +
Sbjct: 259 DAAQAYGKIPIDVNEMNIDLLSISS 282

[Close]

BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot
Match: P87185 (Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.2e-50
Identity = 94/145 (64.83%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 12  LIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGF 71
           +I A PKEI+FTSGATE+NN++IKG   FY++ K+H+ITTQTEHKCVLDS RH+Q   GF
Sbjct: 143 VINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDE-GF 202

Query: 72  DITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHT 131
           ++TYLPV S+G+++L+ L+  IR DT LVS+MAVNNEIGVIQPL+EIGKIC+E  V FHT
Sbjct: 203 EVTYLPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHT 262

Query: 132 DAAQALGEIPIDLEKWNVSLKSIGA 157
           DAAQA G+IPID+ + N+ L SI +
Sbjct: 263 DAAQAYGKIPIDVNEMNIDLLSISS 286

[Close]

BLAST of Csor.00g058670 vs. NCBI nr
Match: KAG6604095.1 (Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 419 bits (1078), Expect = 5.67e-148
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MVGNRIMPSSALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLD 60
           MVGNRIMPSSALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLD
Sbjct: 1   MVGNRIMPSSALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLD 60

Query: 61  SCRHLQQRVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGK 120
           SCRHLQQRVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGK
Sbjct: 61  SCRHLQQRVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGK 120

Query: 121 ICKEFNVPFHTDAAQALGEIPIDLEKWNVSLKSIGALNLTNAFLRRYCVAISIKCPCLQR 180
           ICKEFNVPFHTDAAQALGEIPIDLEKWNVSLKSIGALNLTNAFLRRYCVAISIKCPCLQR
Sbjct: 121 ICKEFNVPFHTDAAQALGEIPIDLEKWNVSLKSIGALNLTNAFLRRYCVAISIKCPCLQR 180

Query: 181 RKQLLKRRGGSAEEAPCAVQWPSFGQSD 208
           RKQLLKRRGGSAEEAPCAVQWPSFGQSD
Sbjct: 181 RKQLLKRRGGSAEEAPCAVQWPSFGQSD 208

[Close]

BLAST of Csor.00g058670 vs. NCBI nr
Match: XP_008440458.1 (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cysteine desulfurase [Cucumis melo var. makuwa] >TYK12780.1 cysteine desulfurase [Cucumis melo var. makuwa])

HSP 1 Score: 266 bits (679), Expect = 7.93e-84
Identity = 133/146 (91.10%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYREKKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 111 AALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQE- 170

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 171 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 230

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 231 HTDAAQALGKIPIDVEKWNVSLMSLS 255

[Close]

BLAST of Csor.00g058670 vs. NCBI nr
Match: KAE8647530.1 (hypothetical protein Csa_002991 [Cucumis sativus])

HSP 1 Score: 265 bits (676), Expect = 9.64e-84
Identity = 132/146 (90.41%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYR+KKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 112 AALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQE- 171

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 172 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 231

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 232 HTDAAQALGKIPIDVEKWNVSLMSLS 256

[Close]

BLAST of Csor.00g058670 vs. NCBI nr
Match: XP_011657914.1 (cysteine desulfurase, mitochondrial [Cucumis sativus])

HSP 1 Score: 265 bits (676), Expect = 2.31e-83
Identity = 132/146 (90.41%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYR+KKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 112 AALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQE- 171

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 172 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 231

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 232 HTDAAQALGKIPIDVEKWNVSLMSLS 256

[Close]

BLAST of Csor.00g058670 vs. NCBI nr
Match: XP_022132842.1 (cysteine desulfurase, mitochondrial [Momordica charantia])

HSP 1 Score: 263 bits (673), Expect = 6.34e-83
Identity = 131/146 (89.73%), Postives = 139/146 (95.21%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNIS+KG M+FYREKKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 111 AALIGASPKEIVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQE- 170

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GFDITYLPVGSDGIVDLEKLRS IRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPF
Sbjct: 171 GFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPF 230

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 231 HTDAAQALGKIPIDVEKWNVSLMSLS 255

[Close]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 266 bits (679), Expect = 3.84e-84
Identity = 133/146 (91.10%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYREKKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 111 AALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQE- 170

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 171 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 230

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 231 HTDAAQALGKIPIDVEKWNVSLMSLS 255

[Close]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 266 bits (679), Expect = 3.84e-84
Identity = 133/146 (91.10%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYREKKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 111 AALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQE- 170

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 171 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 230

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 231 HTDAAQALGKIPIDVEKWNVSLMSLS 255

[Close]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL
Match: A0A0A0KIM0 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1)

HSP 1 Score: 265 bits (676), Expect = 1.12e-83
Identity = 132/146 (90.41%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNISIKG M+FYR+KKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 112 AALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQE- 171

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 172 GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPF 231

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 232 HTDAAQALGKIPIDVEKWNVSLMSLS 256

[Close]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL
Match: A0A6J1BUZ1 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1)

HSP 1 Score: 263 bits (673), Expect = 3.07e-83
Identity = 131/146 (89.73%), Postives = 139/146 (95.21%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNIS+KG M+FYREKKRHLITTQTEHKCVLDSCRHLQQ  
Sbjct: 111 AALIGASPKEIVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQE- 170

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GFDITYLPVGSDGIVDLEKLRS IRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPF
Sbjct: 171 GFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPF 230

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 231 HTDAAQALGKIPIDVEKWNVSLMSLS 255

[Close]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL
Match: A0A6J1HHQ0 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1)

HSP 1 Score: 262 bits (669), Expect = 1.19e-82
Identity = 128/146 (87.67%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 10  SALIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRV 69
           +ALIGASPKEIVFTSGATESNNIS+KG M+FY+EKK+H+ITTQTEHKCVLDSCRHLQQ  
Sbjct: 110 AALIGASPKEIVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQE- 169

Query: 70  GFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPF 129
           GFDITYLPVG+DGIVDLEKLRS IRPDTGLVS+MAVNNEIGVIQP+EEIGKICKEFNVPF
Sbjct: 170 GFDITYLPVGTDGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPF 229

Query: 130 HTDAAQALGEIPIDLEKWNVSLKSIG 155
           HTDAAQALG+IPID+EKWNVSL S+ 
Sbjct: 230 HTDAAQALGKIPIDVEKWNVSLMSLS 254

[Close]

BLAST of Csor.00g058670 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 235.3 bits (599), Expect = 4.3e-62
Identity = 108/145 (74.48%), Postives = 131/145 (90.34%), Query Frame = 0


Query: 12  LIGASPKEIVFTSGATESNNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGF 71
           LI ASPKEIVF SGATE+NN+++KG M+FY++ K+H+ITTQTEHKCVLDSCRHLQQ  GF
Sbjct: 109 LIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQE-GF 168

Query: 72  DITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHT 131
           ++TYLPV +DG+VDLE LR  IRPDTGLVS+MAVNNEIGV+QP+EEIG ICKE NVPFHT
Sbjct: 169 EVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHT 228

Query: 132 DAAQALGEIPIDLEKWNVSLKSIGA 157
           DAAQA+G+IP+D++KWNV+L S+ A
Sbjct: 229 DAAQAIGKIPVDVKKWNVALMSMSA 252

[Close]

BLAST of Csor.00g058670 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 203.8 bits (517), Expect = 1.4e-52
Identity = 91/125 (72.80%), Postives = 113/125 (90.40%), Query Frame = 0


Query: 32  ISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGFDITYLPVGSDGIVDLEKLRS 91
           +++KG M+FY++ K+H+ITTQTEHKCVLDSCRHLQQ  GF++TYLPV +DG+VDLE LR 
Sbjct: 1   MAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQE-GFEVTYLPVKTDGLVDLEMLRE 60

Query: 92  VIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNVSL 151
            IRPDTGLVS+MAVNNEIGV+QP+EEIG ICKE NVPFHTDAAQA+G+IP+D++KWNV+L
Sbjct: 61  AIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVAL 120

Query: 152 KSIGA 157
            S+ A
Sbjct: 121 MSMSA 124

[Close]

BLAST of Csor.00g058670 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 68.2 bits (165), Expect = 9.1e-12
Identity = 38/132 (28.79%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 18  KEIVFTSGATES-NNISIKGGMNFYREKKRHLITTQTEHKCVLDSCRHLQQRVGFDITYL 77
           +EIVFT  ATE+ N ++   G++  +     ++T    H C++   + + Q+ G  + ++
Sbjct: 135 REIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHSCIV-PWQIVSQKTGAVLKFV 194

Query: 78  PVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQA 137
            +  D + D+ KLR +I P T LV+V  V+N +    P+EEI     +       DA Q+
Sbjct: 195 TLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQS 254

Query: 138 LGEIPIDLEKWN 149
           +  + +D++K N
Sbjct: 255 VPHMVVDVQKLN 265

[Close]

BLAST of Csor.00g058670 vs. TAIR 10
Match: AT1G27980.1 (dihydrosphingosine phosphate lyase )

HSP 1 Score: 48.1 bits (113), Expect = 9.7e-06
Identity = 35/172 (20.35%), Postives = 73/172 (42.44%), Query Frame = 0


Query: 23  TSGATESNNISIKGGMNFYREKK----RHLITTQTEHKCVLDSCRHLQQRVGFDITYLPV 82
           TSG TES  +++K   ++ + KK      +I  ++ H     + ++ +      +  +PV
Sbjct: 203 TSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHSAYDKAAQYFK----IKLWRVPV 262

Query: 83  GSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTD------ 142
             D   D++  R  I  +T ++   A     G+I P+EE+G++   + + FH D      
Sbjct: 263 DKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEELGQLALSYGICFHVDLCLGGF 322

Query: 143 -----------------AAQALGEIPIDLEKWNVSLKSIGALNLTNAFLRRY 168
                            + Q +  I +D+ K+ ++ K    +   N  +R++
Sbjct: 323 VLPFARKLGYQIPPFDFSVQGVTSISVDVHKYGLAPKGTSTVLYRNHEIRKH 370

The following BLAST results are available for this feature:

BLAST of Csor.00g058670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49543	6.1e-61	74.48	Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=...	[more]
P0DN31	2.6e-51	63.82	Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-46...	[more]
Q54X04	2.6e-51	66.21	Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum OX=4468...	[more]
P87187	1.7e-50	65.52	Cysteine desulfurase, mitochondrial OS=Candida maltosa OX=5479 GN=SPL1 PE=3 SV=1	[more]
P87185	2.2e-50	64.83	Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MY...	[more]

BLAST of Csor.00g058670 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6604095.1	5.67e-148	100.00	Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_008440458.1	7.93e-84	91.10	PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cyst...	[more]
KAE8647530.1	9.64e-84	90.41	hypothetical protein Csa_002991 [Cucumis sativus]	[more]
XP_011657914.1	2.31e-83	90.41	cysteine desulfurase, mitochondrial [Cucumis sativus]	[more]
XP_022132842.1	6.34e-83	89.73	cysteine desulfurase, mitochondrial [Momordica charantia]	[more]

BLAST of Csor.00g058670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CMX0	3.84e-84	91.10	Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3B0Q9	3.84e-84	91.10	Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1	[more]
A0A0A0KIM0	1.12e-83	90.41	Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1	[more]
A0A6J1BUZ1	3.07e-83	89.73	Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1	[more]
A0A6J1HHQ0	1.19e-82	87.67	Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1	[more]

BLAST of Csor.00g058670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65720.1	4.3e-62	74.48	nitrogen fixation S (NIFS)-like 1	[more]
AT5G65720.2	1.4e-52	72.80	nitrogen fixation S (NIFS)-like 1	[more]
AT1G08490.1	9.1e-12	28.79	chloroplastic NIFS-like cysteine desulfurase	[more]
AT1G27980.1	9.7e-06	20.35	dihydrosphingosine phosphate lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 11..151 e-value: 7.4E-37 score: 127.2
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 11..154 e-value: 4.6E-57 score: 195.7
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE FAMILY MEMBER	coord: 11..156
IPR010240	Cysteine desulfurase IscS	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 11..156
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 11..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g058670.m02	Csor.00g058670.m02	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044571	[2Fe-2S] cluster assembly
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity