Csor.00g049710 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g049710
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr11: 3731245 .. 3735036 (+)
RNA-Seq ExpressionCsor.00g049710
SyntenyCsor.00g049710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTTTGAGACCTCAATTCACGGTGGGCGGTTCAACAACAAGAGGATTGAGTTCAAGCGATGCAATCCGGGCTTCATCTCCACCTACTGGACGATGTTTACAGTGTTCTGGATCATGGCGGTGGCTTCGATGTTGCTCTGGCAGTGCACTATTGATGATGAGTTCCTTTTCCACCGGAGTGTCCCGTTCAGGCCGTTGCCGAGGACGCGGCCTGTTGCGTTTAATTTGACGGATTTTGGAGGAGTGGGCGATGGAGTCACTTTGAATACCGTGGCCTTCGCGAACGCCATTTATGCCATTTCGAAGTGGCGGGCCAGAGGCGGTGGACAGCTTAATGTTCCTCCTGGCCGTTGGCTTACGGCTCCCTTTAATCTCACTAGTCATATGACTTTGTTTCTTGATCGAGATGCTGTTATATTGGGGATTCAGGTCTGTTTCAACTTGAATTTCGTTAAATTTTTTGTTTGAATTTGGTTAGAACTGTGCTGCTGGGAATAACTTGATGGAATTGTTGGCTTCTTCTGTTTAAACTTGTTAGGATCGCCGTAATGTATTAGCAATTACTGTTGTTATTGATACTCATTTTGAATGAAGCTGAGTAGTGAAAATTATCAGTAGATATGCATAGTTTAGGCTATGAATTCGTACTAGATTGGTTGTAAGATCTTGAGTGTTCTTAAAATAAAGAACCTAGAAGCAGCTATCAAGAGCTAGACCTTTGGACATTCCACAATTATGACTGATGAAAGTTTTATCATGTTACGAGAGCTATAGGCGATTGTTCGATTATTTGTGTAGTTACTTGTTCGATTATCTGCTGCCCACGAGAACGTAACTACTTGTGCACTTCCATCCCCATCTTCACCTATTTATCCATGGAAAGATGGTAGTAGTAACGTTAAATTGTTCTTCAACAAGACCGCCCTGCTCGAGTATTGTAAAGGTTTTCGCATGTCGTTTTGAATATGTAATCTTATATGGTCACGTTTGTATTTCAACATTTTGTCTAGTATGCTACCTGTAATAGTTCAAGCCCACCACTAGCAGATATTGTCTGCTTTGACTCGTTATGTATCGTTGTCATCCTCATGGTTTTAAAATGTGTCTACTAGGGAGAGGTTCCAGTACCCTTATAAGGAATGTTTTGTTCCCATTTCCAACCGATGTGATCTCATAGTACCCATCATCCAGTAGTTTCTAGTTAAACTAATGGATTGTTTCGGTCATCTCGTTTAACCATTATAGTGTTTATTATTTCTTCGTTAGTTTCTACAACGAACTTCTAGAAACGGGATGAATGTTGGCTAATCTTATTCATGTCACAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTACGGAAGGGAGCACGCTGGACCTCGTTATGGGAGTTTGATCCATGGTCAAAATCTCATAGATGTCGTGATAACAGGTCAGGTTGATCGAGATTTCTTTGTTTAGGTACTTTTTTGTTAATCCTTGCTTGGGAATCTCAGCATAAACTTGTTGAAGCTTCAACCATTTGTGACTTCTGATTGTGTATATGTCATGACTTACAGGGAATAATGGTACCATAAATGGACAGGGTCAAACATGGTGGAAGAAATTTCGCCAAAAGCTTCTGAACCACACAAGGGGTCCCCTTGTTCAGATCATGTGGTCAAGGGATATTTTGATCAGCAATATAACTTTGCGCGACTCTCCATTTTGGACTCTTCATCCATACGACTGTAAGAACGTTACTATCCGGAATGTTACGATCTTGGCTCCGATACATGATGCACCAAATACAGATGGAATAGATCCTGGTAAGCTTTAGTGCTTTGTTTATATCGTTTCCCAATCGGATGCTTGGTTTAATGTAATGATGCGACTGCTGTATAGTGAACGTAGAGTAGGTTAAGTCGTTCCTTGTGCTGATGTCGATAAATTGCATTAGCTTGTGTATTAGATGAATGTTGGATACCTCGAACTAAGGACCAAAGACCATTTTAGTTTTTCCCATAGTATATGTGAGATCCCACGTCGGTTACGATACCTCGAACTAAGGACCAAAGACCATTTTAGTTTTTCCCATAGTATATGTGAGATCCCACGTCGGTTAGAGAGGGGAGCGTGTGCTAGTGATGACGTTGGGCATCGGAGGGGGTAGATTGTGAGATCCCACATCAGTTAGAGAGGGGAACGAAACATTTTTTTTATAAGGGTGAGACTGACAACGATATGTAATGGGCCAAAGCGGACAATATCTGCTAGCCGTAGGCTTAGGCTGTTAGAGTATAACTTTGTATGCAGAAGATGGGCCTGTAACTCAGTTTTCTAGACTAGGTGGCACCGGCCTAATGACGTGCATTATGATTGAGTTTAAGGGGTAGGATATTGATGCGTTCGGGTTGAGTTTAATTCGATTTGATTTGGAGCTTAACCTAAGTTAGTTGGAAGCATAGGAAAATCATTCTATGTCCTTGTTAAACTACGAACGGGTTTGGAATGACTTTTGAAGCGTTTAAAAACACTTTTTTAAGCACTTAAAAAGTCATTCTAAATCGGACATTAAATTTTTACATGCTTGAACTTTATAAATGGTCATTTGATAAGGGCTTGCTCCATTCTGTATATTTTCATAAACAGATTCATGTGAAGATATGGTGATTGAGGATTGTTACATAAGTGTTGGAGACGATGGCATTGCAATCAAGAGTGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCGAGAAACATACGAATCCGAAACATCGTTGTTCAATCTATGGTTAGGTAAATATTTCGCATCCATTCGTGATCAAAACACATATCAACTAGATTCACGTCGTATGAAAAGATAGCTAACTCATCATTTTGGTTGAAATTCTTCAGTGCTGGGATATCGATCGGTAGTGAGATGTCGGGTGGCGTATCAGGCATCACCATAGAGAATATCGTGGTGTGGAACTCCAGGCGAGGTGTTCGGATCAAGACTGCTCCAGGGAGAGGAGGATATGTGAAAGACATTACTTATCGAAATCTGACCCTCGAAGTTGTACGTGTTGGGATTGTCGTAAAGACAGACTACAACGAACACCCTGACGAAGGATATGACCCAAAGGCAGTTCCACTGCTGAAGGACATAAGCTTCACGAGCATCCATGGGCAGGGAGTTCGAGTGCCCGTTCGAATGCACGGTAGCGAAGACATTCCAGTAAGAAATGTGACATTCAGGGATATGTCAGTGGGAATAACATACAAAAAGAAACAAATATTCCAATGTGCATTTGTTCAAGGACAAGTTATAGGAACCATCTTCCCTACTCCTTGTGTCAACCTCGACCGTTACGACGAGCATGAACGACTAATTAAACGCTCGGCTTCTCAAAATGCAACAGATATCGACTATGACTTTTGAGTAGCAGCTAGCTGATTTGATCAAACCACTAAAAACTGTCTTTTTTTTTTTCTTCTTGTTCTTCTTTTTACACCAGTTGAAGACTCTCATATGTCTATTTTATGTCTGAAATTTGCATTTTTTTGTCTGGTAGAGATGTACAGAGGAACCCTTTTTTTTATTACAATAGATAGCTTTGATTTCTGAGTTATTTCTCTAATGTGTAGGTTCCATTTCATTTGAATGGCCATGGCTGTTCTTCGTTCAACTTGCAGGTAGCAGAGTTTGGTGGTGGTGGGGCTGTGAAAAAGATGGATGTTGGCAAAGTGGGATGGATTGATTGGCAATTTGTACTGAAAGTCAGCTCAAATCATAGGCTTTCTTGA

mRNA sequence

ATGAGGTTTGAGACCTCAATTCACGGTGGGCGGTTCAACAACAAGAGGATTGAGTTCAAGCGATGCAATCCGGGCTTCATCTCCACCTACTGGACGATGTTTACAGTGTTCTGGATCATGGCGGTGGCTTCGATGTTGCTCTGGCAGTGCACTATTGATGATGAGTTCCTTTTCCACCGGAGTGTCCCGTTCAGGCCGTTGCCGAGGACGCGGCCTGTTGCGTTTAATTTGACGGATTTTGGAGGAGTGGGCGATGGAGTCACTTTGAATACCGTGGCCTTCGCGAACGCCATTTATGCCATTTCGAAGTGGCGGGCCAGAGGCGGTGGACAGCTTAATGTTCCTCCTGGCCGTTGGCTTACGGCTCCCTTTAATCTCACTAGTCATATGACTTTGTTTCTTGATCGAGATGCTGTTATATTGGGGATTCAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTACGGAAGGGAGCACGCTGGACCTCGTTATGGGAGTTTGATCCATGGTCAAAATCTCATAGATGTCGTGATAACAGGGAATAATGGTACCATAAATGGACAGGGTCAAACATGGTGGAAGAAATTTCGCCAAAAGCTTCTGAACCACACAAGGGGTCCCCTTGTTCAGATCATGTGGTCAAGGGATATTTTGATCAGCAATATAACTTTGCGCGACTCTCCATTTTGGACTCTTCATCCATACGACTGTAAGAACGTTACTATCCGGAATGTTACGATCTTGGCTCCGATACATGATGCACCAAATACAGATGGAATAGATCCTGATTCATGTGAAGATATGGTGATTGAGGATTGTTACATAAGTGTTGGAGACGATGGCATTGCAATCAAGAGTGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCGAGAAACATACGAATCCGAAACATCGTTGTTCAATCTATGGTTAGTGCTGGGATATCGATCGGTAGTGAGATGTCGGGTGGCGTATCAGGCATCACCATAGAGAATATCGTGGTGTGGAACTCCAGGCGAGGTGTTCGGATCAAGACTGCTCCAGGGAGAGGAGGATATGTGAAAGACATTACTTATCGAAATCTGACCCTCGAAGTTGTACGTGTTGGGATTGTCGTAAAGACAGACTACAACGAACACCCTGACGAAGGATATGACCCAAAGGCAGTTCCACTGCTGAAGGACATAAGCTTCACGAGCATCCATGGGCAGGGAGTTCGAGTGCCCGTTCGAATGCACGGTAGCGAAGACATTCCAGTTCCATTTCATTTGAATGGCCATGGCTGTTCTTCGTTCAACTTGCAGGTAGCAGAGTTTGGTGGTGGTGGGGCTGTGAAAAAGATGGATGTTGGCAAAGTGGGATGGATTGATTGGCAATTTGTACTGAAAGTCAGCTCAAATCATAGGCTTTCTTGA

Coding sequence (CDS)

ATGAGGTTTGAGACCTCAATTCACGGTGGGCGGTTCAACAACAAGAGGATTGAGTTCAAGCGATGCAATCCGGGCTTCATCTCCACCTACTGGACGATGTTTACAGTGTTCTGGATCATGGCGGTGGCTTCGATGTTGCTCTGGCAGTGCACTATTGATGATGAGTTCCTTTTCCACCGGAGTGTCCCGTTCAGGCCGTTGCCGAGGACGCGGCCTGTTGCGTTTAATTTGACGGATTTTGGAGGAGTGGGCGATGGAGTCACTTTGAATACCGTGGCCTTCGCGAACGCCATTTATGCCATTTCGAAGTGGCGGGCCAGAGGCGGTGGACAGCTTAATGTTCCTCCTGGCCGTTGGCTTACGGCTCCCTTTAATCTCACTAGTCATATGACTTTGTTTCTTGATCGAGATGCTGTTATATTGGGGATTCAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTACGGAAGGGAGCACGCTGGACCTCGTTATGGGAGTTTGATCCATGGTCAAAATCTCATAGATGTCGTGATAACAGGGAATAATGGTACCATAAATGGACAGGGTCAAACATGGTGGAAGAAATTTCGCCAAAAGCTTCTGAACCACACAAGGGGTCCCCTTGTTCAGATCATGTGGTCAAGGGATATTTTGATCAGCAATATAACTTTGCGCGACTCTCCATTTTGGACTCTTCATCCATACGACTGTAAGAACGTTACTATCCGGAATGTTACGATCTTGGCTCCGATACATGATGCACCAAATACAGATGGAATAGATCCTGATTCATGTGAAGATATGGTGATTGAGGATTGTTACATAAGTGTTGGAGACGATGGCATTGCAATCAAGAGTGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCGAGAAACATACGAATCCGAAACATCGTTGTTCAATCTATGGTTAGTGCTGGGATATCGATCGGTAGTGAGATGTCGGGTGGCGTATCAGGCATCACCATAGAGAATATCGTGGTGTGGAACTCCAGGCGAGGTGTTCGGATCAAGACTGCTCCAGGGAGAGGAGGATATGTGAAAGACATTACTTATCGAAATCTGACCCTCGAAGTTGTACGTGTTGGGATTGTCGTAAAGACAGACTACAACGAACACCCTGACGAAGGATATGACCCAAAGGCAGTTCCACTGCTGAAGGACATAAGCTTCACGAGCATCCATGGGCAGGGAGTTCGAGTGCCCGTTCGAATGCACGGTAGCGAAGACATTCCAGTTCCATTTCATTTGAATGGCCATGGCTGTTCTTCGTTCAACTTGCAGGTAGCAGAGTTTGGTGGTGGTGGGGCTGTGAAAAAGATGGATGTTGGCAAAGTGGGATGGATTGATTGGCAATTTGTACTGAAAGTCAGCTCAAATCATAGGCTTTCTTGA

Protein sequence

MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHRSVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMHGSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS
Homology
BLAST of Csor.00g049710 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.3e-106
Identity = 183/351 (52.14%), Postives = 245/351 (69.80%), Query Frame = 0

Query: 76  NLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLFLD 135
           +L DFGGVGDG TLNT AF +A+  +SK+ + GG QL VP G+WLT  F+LTSH TLFL 
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 136 RDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGNNGTINGQGQ 195
           RDAV+L  QD   WP++ PLPSYG GR+ A  RY SLI G NL DV+ITG+NGTI+GQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 196 TWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNVTILAP 255
            WW++F    L +TR  L+++M+S DI ISN+TL +SP W +HP   +N+ I+ +TILAP
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAP 243

Query: 256 IHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNIVVQ 315
           +  +PNTDGI+PDSC +  IEDCYI  GDD +A+KSGWD+YGIAYG P++ + IR +   
Sbjct: 244 VR-SPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCI 303

Query: 316 SMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLTLEVVR 375
           S  SA I++GSEMSGG+  +  E+IV  NS  G+RIKT  GRGGYVKDI  R +T++ ++
Sbjct: 304 SPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMK 363

Query: 376 VGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMHGSEDIP 427
               +  +Y  H D  YDPKA P+++ I++  +  + V +  R+ G    P
Sbjct: 364 WAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDP 413

BLAST of Csor.00g049710 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.9e-26
Identity = 106/324 (32.72%), Postives = 151/324 (46.60%), Query Frame = 0

Query: 74  AFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLF 133
           A ++T FG VGDGVT ++ AF  A  A+      G GQ  VP G            MT  
Sbjct: 23  ALDVTQFGAVGDGVTDDSQAFLKAWEAVCS--GTGDGQFVVPAG------------MTFM 82

Query: 134 LDRDAVILGIQDE-KYWPLMPPL------PSYGYGREHAGPRYGSLIHGQNLIDVVITGN 193
           L      L  Q   K  P+   +      PS G  +   G +   ++     I+ ++   
Sbjct: 83  LQP----LKFQGSCKSTPVFVQMLGKLVAPSKGNWK---GDKDQWILFTD--IEGLVIEG 142

Query: 194 NGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVT 253
           +G INGQG +WW+         +R   ++     ++ +S +T  DSP   +H  +C  VT
Sbjct: 143 DGEINGQGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVT 202

Query: 254 IRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRN 313
           I ++ I AP   +PNTDGID  +  ++VI+DC I+ GDD IAI SG           + N
Sbjct: 203 ISSLRINAP-ESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSN 262

Query: 314 IRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWN-----SRRGVRIKTAPGRGGYV 373
           I I  I        GISIGS    G +  T+EN+ V N     +  G RIKT  G  GY 
Sbjct: 263 IHISGI--DCGPGHGISIGSLGKDGETA-TVENVCVQNCNFRGTMNGARIKTWQGGSGYA 302

Query: 374 KDITYRNLTLEVVRVGIVVKTDYN 386
           + IT+  +TL+ V   I++   YN
Sbjct: 323 RMITFNGITLDNVENPIIIDQFYN 302

BLAST of Csor.00g049710 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 2.5e-25
Identity = 98/351 (27.92%), Postives = 163/351 (46.44%), Query Frame = 0

Query: 72  PVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWR----ARGGGQLNVPPGR-WLTAPFNL 131
           P   +++DFG  GDG T +T AF NA      W+    + G   L VP G  +L     L
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA------WKKACSSNGAVNLLVPKGNTYLLKSIQL 124

Query: 132 TSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGN 191
           T            IL +Q      +   L +     ++       +  G N +  V  G+
Sbjct: 125 TGPCN-------SILTVQ------IFGTLSASQKRSDYKDISKWIMFDGVNNLS-VDGGD 184

Query: 192 NGTINGQGQTWWKKF--RQKLLNHTRGPLVQIMW-SRDILISNITLRDSPFWTLHPYDCK 251
            G ++G G+TWW+    R K    T+ P     + S+ +++ N+ +R++    +    C 
Sbjct: 185 TGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCS 244

Query: 252 NVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQP 311
           NV + NV + AP  D+PNTDGI   + +++ + +  I  GDD I+I+SG           
Sbjct: 245 NVQVSNVVVTAPA-DSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG----------- 304

Query: 312 SRNIRIRNIVVQSMVSAGISIGS----EMSGGVSGITIENIVVWNSRRGVRIKTAPGRGG 371
           S+N++I +I        GISIGS         VSG+T++   +  +  GVRIKT  G  G
Sbjct: 305 SQNVQINDITCGP--GHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSG 364

Query: 372 YVKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHG 411
              +I ++N+ ++ V+  I++  DY +      +  AV  +K++ +  I G
Sbjct: 365 TASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISG 380

BLAST of Csor.00g049710 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.0e-23
Identity = 66/169 (39.05%), Postives = 103/169 (60.95%), Query Frame = 0

Query: 228 TLRDSPFWTLHPYDCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGI 287
           T+R++  WT+HP  C+++T    TI+AP HD+PNTDG +P+SC +++I     SVGDD I
Sbjct: 39  TIRNAASWTIHPQGCEDLTAAASTIIAP-HDSPNTDGFNPESCRNVMISGVRFSVGDDCI 98

Query: 288 AIKSG--WDQYGIAYGQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNS 347
           A+K+G         +   +R I +R+ ++Q     G+ IGSEMSGGV  +T+E+  +  +
Sbjct: 99  AVKAGKRGPDGEDDHLAETRGITVRHCLMQP-GHGGLVIGSEMSGGVHDVTVEDCDMIGT 158

Query: 348 RRGVRIKT-APGRGGYVKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYD 394
            RG+R+KT A   GG V +IT R + L+ V+  +     Y+   D G+D
Sbjct: 159 DRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHCDAD-GHD 204

BLAST of Csor.00g049710 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 4.1e-20
Identity = 77/275 (28.00%), Postives = 135/275 (49.09%), Query Frame = 0

Query: 185 GNNGTINGQGQTWWKKFRQ--KLLNHTRGPLVQIMWS-RDILISNITLRDSPFWTLHPYD 244
           G+ G ING G+TWW+   +  K    T+ P    +++ +++ + N+ ++++    +    
Sbjct: 163 GSTGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEK 222

Query: 245 CKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYG 304
           C  V + NV I AP  D+PNTDGI   + +++ + +  I  GDD I+I+ G         
Sbjct: 223 CNKVEVSNVEITAP-GDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDG--------- 282

Query: 305 QPSRNIRIRNIVVQSMVSAGISIGS----EMSGGVSGITIENIVVWNSRRGVRIKTAPGR 364
             ++N++I ++        GISIGS         VSGI ++      S  GVRIKT  G 
Sbjct: 283 --TQNLQIFDLTCGP--GHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGG 342

Query: 365 GGYVKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHG-QGVRVP 424
            G  K+I ++N+ +E V+  I++  DY +  D+  D ++   +K++ + +I G     V 
Sbjct: 343 SGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVA 402

Query: 425 VRMHGSEDIPVPFHLNGHGCSSFNLQVAEFGGGGA 452
           + ++ SE  P         C    L+  +  GG A
Sbjct: 403 ITLNCSEKYP---------CQGIVLENVKIKGGTA 413

BLAST of Csor.00g049710 vs. NCBI nr
Match: KAG6588097.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS
Sbjct: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478

BLAST of Csor.00g049710 vs. NCBI nr
Match: XP_022929222.1 (probable polygalacturonase isoform X2 [Cucurbita moschata])

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 471/478 (98.54%), Postives = 475/478 (99.37%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI++VTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQSVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIEN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPQLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVP HLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQ VLKVSSNHRLS
Sbjct: 421 GSEDIPVPVHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQSVLKVSSNHRLS 478

BLAST of Csor.00g049710 vs. NCBI nr
Match: XP_022967281.1 (probable polygalacturonase isoform X2 [Cucurbita maxima])

HSP 1 Score: 987 bits (2552), Expect = 0.0
Identity = 468/478 (97.91%), Postives = 475/478 (99.37%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKR EFKRC PGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRTEFKRCIPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSH+TLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHLTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI+NVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGIT+EN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP+LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVPFHLNG+GCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNH LS
Sbjct: 421 GSEDIPVPFHLNGYGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHWLS 478

BLAST of Csor.00g049710 vs. NCBI nr
Match: XP_023529364.1 (probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 986 bits (2548), Expect = 0.0
Identity = 465/478 (97.28%), Postives = 475/478 (99.37%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRF TSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFGTSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRP PRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPFPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSR+ILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRNILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI+NVTILAPIHDAPNTDGIDPDSCEDM+IEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQNVTILAPIHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRN+VVQSMVSAGISIGSEMSGGVSGITIEN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNVVVQSMVSAGISIGSEMSGGVSGITIENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTL+VVR+GIVVKTDYNEHPDEG+DPKAVP+LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLDVVRIGIVVKTDYNEHPDEGHDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVP HLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS
Sbjct: 421 GSEDIPVPVHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478

BLAST of Csor.00g049710 vs. NCBI nr
Match: KAG7021986.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPV 427
           GSEDIPV
Sbjct: 421 GSEDIPV 427

BLAST of Csor.00g049710 vs. ExPASy TrEMBL
Match: A0A6J1EM70 (probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435871 PE=3 SV=1)

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 471/478 (98.54%), Postives = 475/478 (99.37%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI++VTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQSVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIEN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPQLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVP HLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQ VLKVSSNHRLS
Sbjct: 421 GSEDIPVPVHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQSVLKVSSNHRLS 478

BLAST of Csor.00g049710 vs. ExPASy TrEMBL
Match: A0A6J1HRK1 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466843 PE=3 SV=1)

HSP 1 Score: 987 bits (2552), Expect = 0.0
Identity = 468/478 (97.91%), Postives = 475/478 (99.37%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKR EFKRC PGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRTEFKRCIPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSH+TLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHLTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI+NVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGIT+EN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP+LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPVPFHLNGHGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHRLS 478
           GSEDIPVPFHLNG+GCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNH LS
Sbjct: 421 GSEDIPVPFHLNGYGCSSFNLQVAEFGGGGAVKKMDVGKVGWIDWQFVLKVSSNHWLS 478

BLAST of Csor.00g049710 vs. ExPASy TrEMBL
Match: A0A6J1ERG4 (probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435871 PE=3 SV=1)

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 422/427 (98.83%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHMTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI++VTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQSVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIEN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPQLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPV 427
           GSEDIPV
Sbjct: 421 GSEDIPV 427

BLAST of Csor.00g049710 vs. ExPASy TrEMBL
Match: A0A6J1HQD2 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466843 PE=3 SV=1)

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 419/427 (98.13%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           MRFETSIHGGRFNNKR EFKRC PGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR
Sbjct: 1   MRFETSIHGGRFNNKRTEFKRCIPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
           SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL
Sbjct: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSH+TLFLD+DAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID
Sbjct: 121 TAPFNLTSHLTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY
Sbjct: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTI+NVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIQNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGIT+EN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVP+LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPV 427
           GSEDIPV
Sbjct: 421 GSEDIPV 427

BLAST of Csor.00g049710 vs. ExPASy TrEMBL
Match: A0A6J1JBR3 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111485343 PE=3 SV=1)

HSP 1 Score: 785 bits (2027), Expect = 1.28e-283
Identity = 373/427 (87.35%), Postives = 396/427 (92.74%), Query Frame = 0

Query: 1   MRFETSIHGGRFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFHR 60
           M  ETSI GGRFNN+RI+FKRC P F STY T+FTV WI AVA++ LW  TI D F F R
Sbjct: 1   MMVETSILGGRFNNQRIDFKRCVPAFFSTYRTLFTVLWIAAVATIFLWMSTIRDGFFFLR 60

Query: 61  SVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWL 120
            V  RPLPR RPVAFNLTDFG VGDGVTLNT AF  AI AISK R++GGGQLNVP GRWL
Sbjct: 61  PVSSRPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRSKGGGQLNVPSGRWL 120

Query: 121 TAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLID 180
           TAPFNLTSHMTLFLD DAVILGIQDEKYWPLMPPLPSYGYGREH GPRYGSLIHGQNL D
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240
           VVITG+NGTINGQGQTWWKK+RQKLLNHTRGPLVQIMWSRDI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIRNVTILAP+HDAPNTDGIDPDSCEDM+IEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGY 360
            QPS+NIRIRN+V+QSMVSAGISIGSEMSGGVSGIT+EN+VVWNSRRGVRIKTAPGRGGY
Sbjct: 301 RQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMH 420
           V+DI+YRNLTL+VVRVGIV+KTDYNEHPDEGYDPKAVP+LKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDISYRNLTLDVVRVGIVIKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEDIPV 427
           GSE+IPV
Sbjct: 421 GSEEIPV 427

BLAST of Csor.00g049710 vs. TAIR 10
Match: AT1G19170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 646.0 bits (1665), Expect = 2.4e-185
Identity = 302/430 (70.23%), Postives = 359/430 (83.49%), Query Frame = 0

Query: 11  RFNNKRIEFKRCNPGFISTYWTMFTVFWIMAVASMLLWQCT-----------IDDEF-LF 70
           +F+ +R++ KR     ++++ T+FT  WI A  S+ LWQ T           +  +F +F
Sbjct: 11  QFHLQRLDPKRRLTSLLASHKTLFTFLWITAFGSVFLWQRTAYIEGGSGTGPVGGKFTIF 70

Query: 71  HRSVPFRPLPRTRPVAFNLTDFGGVGDGVTLNTVAFANAIYAISK-WRARGGGQLNVPPG 130
            +  P +P+PR RPV F+L DFGGVGDG TLNT AF  A+ +ISK   + GGGQLNVPPG
Sbjct: 71  GKIKPIQPIPRLRPVVFDLKDFGGVGDGFTLNTEAFERAVISISKLGGSSGGGQLNVPPG 130

Query: 131 RWLTAPFNLTSHMTLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQN 190
           RWLTAPFNLTSHMTLFL  D+ ILG++DEKYWPLMPPLPSYGYGRE  GPRYGSLIHGQN
Sbjct: 131 RWLTAPFNLTSHMTLFLAEDSEILGVEDEKYWPLMPPLPSYGYGRERPGPRYGSLIHGQN 190

Query: 191 LIDVVITGNNGTINGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTL 250
           L D+VITG+NGTINGQGQ+WWKK +++LLN+TRGPLVQIMWS DI+I+NIT+RDSPFWTL
Sbjct: 191 LKDIVITGHNGTINGQGQSWWKKHQRRLLNYTRGPLVQIMWSSDIVIANITMRDSPFWTL 250

Query: 251 HPYDCKNVTIRNVTILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYG 310
           HPYDCKNVTIRNVTILAP+  APNTDGIDPDSCEDMVIEDCYIS GDD IAIKSGWDQ+G
Sbjct: 251 HPYDCKNVTIRNVTILAPVTGAPNTDGIDPDSCEDMVIEDCYISTGDDAIAIKSGWDQFG 310

Query: 311 IAYGQPSRNIRIRNIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGR 370
           IAYG+PS NI IRN+VV+S++SAG+SIGSEMSGG+S +TIEN+++WNSRRG+RIKTAPGR
Sbjct: 311 IAYGRPSTNILIRNLVVRSVISAGVSIGSEMSGGISNVTIENLLIWNSRRGIRIKTAPGR 370

Query: 371 GGYVKDITYRNLTLEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPV 428
           GGY+++ITY+NLTL+ VRVGIV+KTDYNEH D+ YD KA P+L   SF  IHGQGVRVPV
Sbjct: 371 GGYIRNITYKNLTLDNVRVGIVIKTDYNEHADDNYDRKAYPILSGFSFAGIHGQGVRVPV 430

BLAST of Csor.00g049710 vs. TAIR 10
Match: AT3G42950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 636.3 bits (1640), Expect = 1.9e-182
Identity = 296/414 (71.50%), Postives = 350/414 (84.54%), Query Frame = 0

Query: 26  FISTYWTMFTVFWIMAVASMLLWQCTIDDEFLFH------------RSVPFRPLPRTRPV 85
           F+S++ ++ TVFWI   AS+ +WQ       L+              +V     P+ RPV
Sbjct: 5   FLSSHKSLITVFWIATFASLFIWQFGGVSTNLYSGFSLFWSSSSTTTTVFSGGFPKLRPV 64

Query: 86  AFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLF 145
            FNLTDFG VGDGVT+NT AF  AIY ISK   +GGGQLNVPPGRWLTAPFNLTS+MTLF
Sbjct: 65  VFNLTDFGAVGDGVTINTEAFEKAIYKISKLAKKGGGQLNVPPGRWLTAPFNLTSYMTLF 124

Query: 146 LDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGNNGTINGQ 205
           L  +A IL +QDEKYW L+PPLPSYGYGREH GPRYGS IHGQNL DVV+TGNNG+INGQ
Sbjct: 125 LSENAEILALQDEKYWSLLPPLPSYGYGREHHGPRYGSFIHGQNLRDVVVTGNNGSINGQ 184

Query: 206 GQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNVTIL 265
           GQTWWKK+RQKLLNHTRGPLVQIMWS DI+ +NITLRDSPFWTLHPYDCKNVTI N+TIL
Sbjct: 185 GQTWWKKYRQKLLNHTRGPLVQIMWSSDIVFANITLRDSPFWTLHPYDCKNVTITNMTIL 244

Query: 266 APIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNIV 325
           AP+ +APNTDGIDPDSCEDM+IE+ YISVGDDGIAIKSGWDQYG  YG+PS+NI IRN++
Sbjct: 245 APVFEAPNTDGIDPDSCEDMLIENSYISVGDDGIAIKSGWDQYGTTYGKPSKNILIRNLI 304

Query: 326 VQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLTLEV 385
           ++SMVSAGISIGSEMSGGVS IT+ENI++W+SRRGVRIKTAPGRGGYV+DIT+RN+TL+ 
Sbjct: 305 IRSMVSAGISIGSEMSGGVSNITVENILIWSSRRGVRIKTAPGRGGYVRDITFRNVTLDE 364

Query: 386 VRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMHGSEDIPV 428
           +RVGIV+KTDYNEHPD G++P+A P+L++I++T I+GQGVRVPVR+ GS++IPV
Sbjct: 365 LRVGIVIKTDYNEHPDGGFNPQAFPILENINYTGIYGQGVRVPVRIQGSKEIPV 418

BLAST of Csor.00g049710 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 399.4 bits (1025), Expect = 4.0e-111
Identity = 186/353 (52.69%), Postives = 259/353 (73.37%), Query Frame = 0

Query: 71  RPVAFNLTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHM 130
           RP + ++T+FG VGDGVTLNT AF NA++ ++ +  +GG +L VP G+WLT  F+L SH+
Sbjct: 39  RPHSVSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHL 98

Query: 131 TLFLDRDAVILGIQDEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGNNGTI 190
           TL+LD+ A ILG    + WP++ PLPSYG GRE  G R+ SLI+GQNL DVVITG NGTI
Sbjct: 99  TLWLDKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTI 158

Query: 191 NGQGQTWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNV 250
           +GQG  WW  FR   LN+TR  LV++M S  ++ISN+T  +SPFW +HP  C++V ++N+
Sbjct: 159 DGQGTVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNL 218

Query: 251 TILAPIHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIR 310
           TILAP+ ++PNTDG+DPDS  ++ IEDCYI  GDD ++IKSGWD+YGI+Y +PS  I+I 
Sbjct: 219 TILAPL-ESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKIN 278

Query: 311 NIVVQSMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLT 370
            +  Q+  S+GI+IGSEMSGGVS I I+++ ++NS  G+RIKT+ GRGGYV+++   N+ 
Sbjct: 279 RLTGQTTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVK 338

Query: 371 LEVVRVGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMHGSE 424
           L+ V+  I     Y EHPDE YDPKA+P ++ I+F +++G G+ V   + G E
Sbjct: 339 LDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIE 390

BLAST of Csor.00g049710 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.6e-110
Identity = 187/351 (53.28%), Postives = 247/351 (70.37%), Query Frame = 0

Query: 77  LTDFGGVGDGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLFLDR 136
           LTDFG VGDG T NT AF NAI  +S+    GG QL VPPG+WLT  FNLTSH TLF+ R
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 137 DAVILGIQDEKYWPLMPPLPSYGYGREHAGP-RYGSLIHGQNLIDVVITGNNGTINGQGQ 196
            A IL  QDE  WP++ PLPSYG GR+  G  R+ SLI G NL DVVITGNNGTINGQGQ
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 197 TWWKKFRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNVTILAP 256
            WW KF++K    TR  L++I++S++I ISNITL DSP W +HP  C +V +++VT+LAP
Sbjct: 167 YWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAP 226

Query: 257 IHDAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNIVVQ 316
           +   PNTDGI+PDSC + +IEDCYI  GDD IA+KSGWDQYGI +G P++ + IR +   
Sbjct: 227 V-TVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCI 286

Query: 317 SMVSAGISIGSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLTLEVVR 376
           S  SAG+++GSEMSGG+  + IE++ + N+   +RIKTA GRG YVKDI  R +T++ ++
Sbjct: 287 SPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMK 346

Query: 377 VGIVVKTDYNEHPDEGYDPKAVPLLKDISFTSIHGQGVRVPVRMHGSEDIP 427
               +  +Y  HPDEG+DPKA+P + +I++  +  + V +   + G +  P
Sbjct: 347 YVFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDP 396

BLAST of Csor.00g049710 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.4e-110
Identity = 198/384 (51.56%), Postives = 262/384 (68.23%), Query Frame = 0

Query: 33  MFTVFWIMAVASML--------LWQCTIDDEFLFHRSVPFRPLPRTRPVAFNLTDFGGVG 92
           +  VF ++A+ S+         LW  T   +     S  F   P    +  ++TDFGGVG
Sbjct: 22  LLLVFTVLAILSLQISSNSFLPLWIPTSQYDDPVTCSGFFNHDPFPNRIVMSITDFGGVG 81

Query: 93  DGVTLNTVAFANAIYAISKWRARGGGQLNVPPGRWLTAPFNLTSHMTLFLDRDAVILGIQ 152
           DG T NT AF  A+  +  + A GG QLNVP G WL+  FNLTS+ TLFL+R A+ILG +
Sbjct: 82  DGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLERGALILGSK 141

Query: 153 DEKYWPLMPPLPSYGYGREHAGPRYGSLIHGQNLIDVVITGNNGTINGQGQTWWKKFRQK 212
           D   WP++ PLPSYG GRE  G R+ SLIHG NL +VVITG NGTI+GQG+ WW+ +  +
Sbjct: 142 DLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGKMWWELWWNR 201

Query: 213 LLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPYDCKNVTIRNVTILAPIHDAPNTDG 272
            L HTRG L+++  S +ILISN+TL +SPFWT+HP  C NV IRN+TILAP+ +APNTDG
Sbjct: 202 TLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILAPM-NAPNTDG 261

Query: 273 IDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNIVVQSMVSAGISI 332
           IDPDS  ++ IEDCYI  GDD +A+KSGWDQYG+A  +PS NI IR I   +   +G+ I
Sbjct: 262 IDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRISGTTRTCSGVGI 321

Query: 333 GSEMSGGVSGITIENIVVWNSRRGVRIKTAPGRGGYVKDITYRNLTLEVVRVGIVVKTDY 392
           GSEMSGG+  IT+E+I VW+S  G+RIKT  GRGGY+ +IT+ N+ LE V+V I   +  
Sbjct: 322 GSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLEKVKVPIRFSSGS 381

Query: 393 NEHPDEGYDPKAVPLLKDISFTSI 409
           N+H D+ +DPKA+P +K I  +++
Sbjct: 382 NDHSDDKWDPKALPRVKGIYISNV 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL31.3e-10652.14Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW072.9e-2632.72Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q8RY292.5e-2527.92Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P276444.0e-2339.05Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1[more]
O231474.1e-2028.00Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6588097.10.0100.00putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022929222.10.098.54probable polygalacturonase isoform X2 [Cucurbita moschata][more]
XP_022967281.10.097.91probable polygalacturonase isoform X2 [Cucurbita maxima][more]
XP_023529364.10.097.28probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG7021986.10.0100.00putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1EM700.098.54probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
A0A6J1HRK10.097.91probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146684... [more]
A0A6J1ERG40.098.83probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
A0A6J1HQD20.098.13probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146684... [more]
A0A6J1JBR31.28e-28387.35probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111485343 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G19170.12.4e-18570.23Pectin lyase-like superfamily protein [more]
AT3G42950.11.9e-18271.50Pectin lyase-like superfamily protein [more]
AT3G62110.14.0e-11152.69Pectin lyase-like superfamily protein [more]
AT3G48950.12.6e-11053.28Pectin lyase-like superfamily protein [more]
AT4G33440.13.4e-11051.56Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 304..326
e-value: 64.0
score: 12.4
coord: 361..382
e-value: 640.0
score: 8.6
coord: 270..291
e-value: 13.0
score: 14.7
coord: 332..353
e-value: 1.4
score: 17.9
coord: 242..269
e-value: 140.0
score: 11.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 166..412
e-value: 6.2E-47
score: 160.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 65..456
e-value: 5.2E-117
score: 393.3
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 23..427
NoneNo IPR availablePANTHERPTHR31339:SF44POLYGALACTURONASE-RELATEDcoord: 23..427
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 66..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g049710.m01Csor.00g049710.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity