Csor.00g044810 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g044810
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncytochrome P450 71A1-like
LocationCsor_Chr18: 1148659 .. 1149678 (-)
RNA-Seq ExpressionCsor.00g044810
SyntenyCsor.00g044810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCCCAACGAAGATCCACCAACAATTTCAAGCTCTCAATCCCATCTCTTCATCTCTGTGCCTTTTCTTCTTCTTCATACTTCTGCTAAAGCTCTACAAACGCAAGAAACCCAACTTCCCTCCTTCACCACCAAGGCTACCCATCATAGGCAACCTCCACCAATTAGGTGCCCTGCCACACCAATCCATGGCAGCTCTTTCCAATAAATATGGCCCTCTAATGCTCCTAAAGCTTGGCCAGAAGCATACCCTTGTGGTTTCATCAGCAAAAATGGCTAGAGAAGTGATGAAGACCCATGACATCAAATTCTCCAACAGGCCCCAAACTACTGCCGCATACGAATTGCTGTACCAATGCCAAGACATTGGCTTTACTCAATATGGTGAGTATTGGAGACAAGCCAGGAAAGTGTGCGCTCTTGAGCTTTTTAGTGCCAAAAGAGTGGACTCTTTTCAGTACGTAAGGGATGATGAAATTGGTGGGCTGATGGACAGGATTGGTAAGGCTTGTGTGGGCGTTGAGGCTGTGAATCTAAGCCAATTGTTTCTTCAAACCTCGAACAATATAGTGTCTAGATGTGTTTTGGGAGAGAAATTTGAAGATGAAAATGGGAATAGTAGATTTGGAGATGCAGCCAGAAAGGTTATGGTGCTAATGGTGGCGTTTTGTGTAGCTGATCTTTTTCCTTCTCTTTGGTGGATTGATACTATTAGAGGGTTCAATAAGCAGTTGGAAGATTGCTTCAAAAGATTGGATACATTTTTTAGTAAAGTGGTTGAAGAACACAAAGAAAAAATAAGAGATGATGTTGGAACTGATGAATCTAAGAAGGATTTTGTGGATATCATGTTGCAGCTCCAACGGGATGGCATGGTTGACTATCATTTCTCTCCAATCAACCTCAAAGCAATCGTACTGGTTCGTTTTTTTGTCCATTTAACCCTCAAAGCAATCGTATTAGTTTGTTTTTGTCCATTTAACCGGTATGGTTCCACCCAAAAGAATCCCGTTTAA

mRNA sequence

ATGGATTTCCCAACGAAGATCCACCAACAATTTCAAGCTCTCAATCCCATCTCTTCATCTCTGTGCCTTTTCTTCTTCTTCATACTTCTGCTAAAGCTCTACAAACGCAAGAAACCCAACTTCCCTCCTTCACCACCAAGGCTACCCATCATAGGCAACCTCCACCAATTAGGTGCCCTGCCACACCAATCCATGGCAGCTCTTTCCAATAAATATGGCCCTCTAATGCTCCTAAAGCTTGGCCAGAAGCATACCCTTGTGGTTTCATCAGCAAAAATGGCTAGAGAAGTGATGAAGACCCATGACATCAAATTCTCCAACAGGCCCCAAACTACTGCCGCATACGAATTGCTGTACCAATGCCAAGACATTGGCTTTACTCAATATGGTGAGTATTGGAGACAAGCCAGGAAAGTGTGCGCTCTTGAGCTTTTTAGTGCCAAAAGAGTGGACTCTTTTCAGTACGTAAGGGATGATGAAATTGGTGGGCTGATGGACAGGATTGGTAAGGCTTGTGTGGGCGTTGAGGCTGTGAATCTAAGCCAATTGTTTCTTCAAACCTCGAACAATATAGTGTCTAGATGTGTTTTGGGAGAGAAATTTGAAGATGAAAATGGGAATAGTAGATTTGGAGATGCAGCCAGAAAGGTTATGGTGCTAATGGTGGCGTTTTGTGTAGCTGATCTTTTTCCTTCTCTTTGGTGGATTGATACTATTAGAGGGTTCAATAAGCAGTTGGAAGATTGCTTCAAAAGATTGGATACATTTTTTAGTAAAGTGGTTGAAGAACACAAAGAAAAAATAAGAGATGATGTTGGAACTGATGAATCTAAGAAGGATTTTGTGGATATCATGTTGCAGCTCCAACGGGATGGCATGGTTGACTATCATTTCTCTCCAATCAACCTCAAAGCAATCGTACTGGTTCGTTTTTTTGTCCATTTAACCCTCAAAGCAATCGTATTAGTTTGTTTTTGTCCATTTAACCGGTATGGTTCCACCCAAAAGAATCCCGTTTAA

Coding sequence (CDS)

ATGGATTTCCCAACGAAGATCCACCAACAATTTCAAGCTCTCAATCCCATCTCTTCATCTCTGTGCCTTTTCTTCTTCTTCATACTTCTGCTAAAGCTCTACAAACGCAAGAAACCCAACTTCCCTCCTTCACCACCAAGGCTACCCATCATAGGCAACCTCCACCAATTAGGTGCCCTGCCACACCAATCCATGGCAGCTCTTTCCAATAAATATGGCCCTCTAATGCTCCTAAAGCTTGGCCAGAAGCATACCCTTGTGGTTTCATCAGCAAAAATGGCTAGAGAAGTGATGAAGACCCATGACATCAAATTCTCCAACAGGCCCCAAACTACTGCCGCATACGAATTGCTGTACCAATGCCAAGACATTGGCTTTACTCAATATGGTGAGTATTGGAGACAAGCCAGGAAAGTGTGCGCTCTTGAGCTTTTTAGTGCCAAAAGAGTGGACTCTTTTCAGTACGTAAGGGATGATGAAATTGGTGGGCTGATGGACAGGATTGGTAAGGCTTGTGTGGGCGTTGAGGCTGTGAATCTAAGCCAATTGTTTCTTCAAACCTCGAACAATATAGTGTCTAGATGTGTTTTGGGAGAGAAATTTGAAGATGAAAATGGGAATAGTAGATTTGGAGATGCAGCCAGAAAGGTTATGGTGCTAATGGTGGCGTTTTGTGTAGCTGATCTTTTTCCTTCTCTTTGGTGGATTGATACTATTAGAGGGTTCAATAAGCAGTTGGAAGATTGCTTCAAAAGATTGGATACATTTTTTAGTAAAGTGGTTGAAGAACACAAAGAAAAAATAAGAGATGATGTTGGAACTGATGAATCTAAGAAGGATTTTGTGGATATCATGTTGCAGCTCCAACGGGATGGCATGGTTGACTATCATTTCTCTCCAATCAACCTCAAAGCAATCGTACTGGTTCGTTTTTTTGTCCATTTAACCCTCAAAGCAATCGTATTAGTTTGTTTTTGTCCATTTAACCGGTATGGTTCCACCCAAAAGAATCCCGTTTAA

Protein sequence

MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVLVRFFVHLTLKAIVLVCFCPFNRYGSTQKNPV
Homology
BLAST of Csor.00g044810 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-74
Identity = 134/287 (46.69%), Postives = 195/287 (67.94%), Query Frame = 0

Query: 26  FFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLMLLKLGQKHT 85
           FF+L L   + KKPN PPSPP LPIIGNLHQLG LPH+S+ +L+N+ GPL+LL LG   T
Sbjct: 16  FFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPT 75

Query: 86  LVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQARKVCALELF 145
           L+VS+A++A E++KTHD+ F++RP TTAA  + Y C D+ F+ YGEYWRQ RK+C LEL 
Sbjct: 76  LIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELL 135

Query: 146 SAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVLGEKFE-DE 205
           S KRV+S++ +R++E+G +M+RI ++C   EAVNLS+L L  S+  ++R   G+K+E +E
Sbjct: 136 SIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEE 195

Query: 206 NGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFFSKVVEEH 265
              ++F D A ++  LM AF V D FPS  W+D + G + +L+     LD F   V+++H
Sbjct: 196 ERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDH 255

Query: 266 KEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVLVRF 312
               + +      +KD VD++L LQ+D  +  H +  NLKA++L  F
Sbjct: 256 LLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMF 302

BLAST of Csor.00g044810 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 139/294 (47.28%), Postives = 187/294 (63.61%), Query Frame = 0

Query: 21  LCLFFFFILLL-----KLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPL 80
           LCL  F +  L        K +K   PPSPPRLP IGNLHQLG+ PH+S+ ALS KYG +
Sbjct: 31  LCLSIFILFSLTRSSSPSEKNRKLKLPPSPPRLPWIGNLHQLGSFPHRSLRALSKKYGDV 90

Query: 81  MLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQ 140
           M +  G+  TL+VSSA+MA++VMKT DI F +RPQTTA   L Y   DI F  YGEYWRQ
Sbjct: 91  MFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTTAPSILFYDGHDIAFAPYGEYWRQ 150

Query: 141 ARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEA--VNLSQLFLQTSNNIVS 200
            R++C LEL S KRV  FQY R +E+  L+ +I KA        +NL +L + TSNNI+ 
Sbjct: 151 VRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKASASANGAPINLGELLVSTSNNIIC 210

Query: 201 RCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRL 260
           RC+LG+KFED+  N  FG+  +++M  +++F   D FPSL WID  RG+   L+  +   
Sbjct: 211 RCILGQKFEDKEDN-WFGETTKELMTQVMSFSFGDFFPSLKWIDRARGYLAYLKSIWLEF 270

Query: 261 DTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIV 308
           D FF K+++EHK   ++       KKD VDI+L +Q DG +D+  +  N+KAI+
Sbjct: 271 DKFFDKLIDEHKAAQKEG---KPRKKDIVDILLDVQNDGSLDFELTTSNVKAIL 320

BLAST of Csor.00g044810 vs. ExPASy Swiss-Prot
Match: Q9STL1 (Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 122/289 (42.21%), Postives = 182/289 (62.98%), Query Frame = 0

Query: 20  SLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLMLLK 79
           SL +F   +  +K  K KK N P SPPRLP+IGNLHQLG  PH+S+ +LSN+YGPLMLL+
Sbjct: 11  SLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLR 70

Query: 80  LGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQARKV 139
            G    LVVSSA +AR+++KT+D  F++RP++    ++ Y+ +D+    YGEYWRQ + V
Sbjct: 71  FGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSV 130

Query: 140 CALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVLGE 199
           C L L + K V SF+ VR +EI  +M++I K+      VNLS+L    +N+++SR  LG 
Sbjct: 131 CVLHLLTNKMVRSFRNVRQEEISLMMEKIQKS--SSLQVNLSELLGSLTNDVISRVALGR 190

Query: 200 KFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFFSK 259
           K+ DE   + F +  +++  L+  FCV    P L WID I G + QL+     LD F  K
Sbjct: 191 KYSDE---TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEK 250

Query: 260 VVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVL 309
           VV++H++        D  + DFVD++L++QR+  V +    +++KAI+L
Sbjct: 251 VVQDHED-------GDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIIL 287

BLAST of Csor.00g044810 vs. ExPASy Swiss-Prot
Match: Q9STL2 (Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.2e-59
Identity = 125/292 (42.81%), Postives = 179/292 (61.30%), Query Frame = 0

Query: 17  ISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLM 76
           I  SL +F   +   K  + KK N P SPPRLP+IGNLHQLG  PH+S+ +LS++YGPLM
Sbjct: 8   ILQSLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHRYGPLM 67

Query: 77  LLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQA 136
           LL LG+   LVVSSA +AR+++KTHD  F++RP++    +L Y  +D+ F  YGEYWRQ 
Sbjct: 68  LLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYWRQI 127

Query: 137 RKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCV 196
           + VC L L S K V SF+ VR +EI  +M++I K+      VN+S+L    +N+++SR  
Sbjct: 128 KSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKS--SSLQVNVSELLGSLTNDVISRIA 187

Query: 197 LGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTF 256
           LG K+  E  +    +  +++M+LM  F V    P L WID I G + QL      LD F
Sbjct: 188 LGRKYSGETDSK---ELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEF 247

Query: 257 FSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVL 309
             KVV++H       V  D  + DFVD++L++QR+  + +    + +KAIVL
Sbjct: 248 LEKVVQDH-------VDGDGQRTDFVDVLLRIQREKSIGFEIDRLCIKAIVL 287

BLAST of Csor.00g044810 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 4.1e-59
Identity = 122/305 (40.00%), Postives = 181/305 (59.34%), Query Frame = 0

Query: 21  LCLFFFFILLLKLYKRK---KPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLML 80
           L LF  FI  + + KRK   KPN PPSPP+LPI+GN HQLG L H+++  L+ KYGPLML
Sbjct: 7   LTLFLLFISTVFILKRKFSHKPNLPPSPPKLPILGNFHQLGTLVHRAVTVLAAKYGPLML 66

Query: 81  LKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQAR 140
           L  G+   L+VSS + A+E+MKTHD+  +NRP TTAA  LLY C DI F  YGEYWR+ +
Sbjct: 67  LHFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREMK 126

Query: 141 KVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVL 200
           K+  L L S K++ SF+ VR++    ++  I +       V+++ +    S +++ RC L
Sbjct: 127 KMAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRCTL 186

Query: 201 GEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFF 260
           G K     G  +F   +R  + L+ AFC  D FP + W+D + G N++L+   + LD F 
Sbjct: 187 GFK---PKGQHKFQKLSRDFLDLVGAFCFNDFFPGMAWMDALTGLNRKLKKGSRELDDFV 246

Query: 261 SKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVLVRFFVHLTL 320
            +++EE    ++D V  +E    F+D++L   RD   +   +  N+KAI+L  F   + L
Sbjct: 247 DELIEERIAMVKDGVEPNE----FLDLLLHTHRDTTQEIKLTRDNVKAIILDTFLGGIDL 304

Query: 321 KAIVL 323
            A V+
Sbjct: 307 PASVM 304

BLAST of Csor.00g044810 vs. NCBI nr
Match: KAG6573092.1 (Phenylacetaldehyde oxime monooxygenase CYP71AN24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 683 bits (1762), Expect = 7.56e-248
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL
Sbjct: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP
Sbjct: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300

Query: 301 INLKAIVLVRFFVHLTLKAIVLVCFCPFNRYGSTQKNPV 339
           INLKAIVLVRFFVHLTLKAIVLVCFCPFNRYGSTQKNPV
Sbjct: 301 INLKAIVLVRFFVHLTLKAIVLVCFCPFNRYGSTQKNPV 339

BLAST of Csor.00g044810 vs. NCBI nr
Match: XP_022954772.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 607 bits (1564), Expect = 5.31e-215
Identity = 304/311 (97.75%), Postives = 305/311 (98.07%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MDFPTKIHQQFQALNPISSSLCLFFFFI+LLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDFPTKIHQQFQALNPISSSLCLFFFFIVLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKT DIKFSNRPQTTAAYELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTQDIKFSNRPQTTAAYELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRI KACVGVEAVNL
Sbjct: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIRKACVGVEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNKQLEDCFKRLDTFFSKVVEEHKEKIRD V TDESKKDFVDIMLQLQRDGMVDYHFSP
Sbjct: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDGVLTDESKKDFVDIMLQLQRDGMVDYHFSP 300

Query: 301 INLKAIVLVRF 311
           INLKAIVL  F
Sbjct: 301 INLKAIVLDMF 311

BLAST of Csor.00g044810 vs. NCBI nr
Match: XP_023542640.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575 bits (1482), Expect = 3.38e-202
Identity = 285/311 (91.64%), Postives = 295/311 (94.86%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MDFPTKI QQFQALNPIS SLCLFFFFI+LLKL+KR KPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 23  MDFPTKIQQQFQALNPISFSLCLFFFFIVLLKLFKRNKPNFPPSPPRLPIIGNLHQLGAL 82

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKT+DIKFSNRPQTTAAYELLYQ
Sbjct: 83  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTYDIKFSNRPQTTAAYELLYQ 142

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGFTQYGEYWRQARKVCALELFS KRVD FQYVRD+E+G L+DRIGKAC+G E VNL
Sbjct: 143 CQDIGFTQYGEYWRQARKVCALELFSVKRVDFFQYVRDEEVGELIDRIGKACMGGEGVNL 202

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLF QTSNNIVSRCVLGEKFEDENGNSRFGD ARKVMVL+ AFCVADLFP LWWIDTIR
Sbjct: 203 SQLFPQTSNNIVSRCVLGEKFEDENGNSRFGDTARKVMVLITAFCVADLFPCLWWIDTIR 262

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DCFKRLDTFFSKVVEEHKEKIRD VGTDESKKDFVDIMLQLQRDGMVDYHFSP
Sbjct: 263 GFNKELKDCFKRLDTFFSKVVEEHKEKIRDGVGTDESKKDFVDIMLQLQRDGMVDYHFSP 322

Query: 301 INLKAIVLVRF 311
            NLKAIVL  F
Sbjct: 323 DNLKAIVLDMF 333

BLAST of Csor.00g044810 vs. NCBI nr
Match: XP_022954771.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 549 bits (1414), Expect = 3.75e-192
Identity = 272/311 (87.46%), Postives = 287/311 (92.28%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD   KI QQFQALNPIS SLCLFFFF+LLLKL++RKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDLTRKIQQQFQALNPISLSLCLFFFFVLLLKLFERKKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKH LVVSS KMAREVMKTHDIKFSNRPQTTAA+ELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGF QYGEYWRQARKVCALELFS KRVDSFQYVRD+E+GGLMDRI KACVG EAVNL
Sbjct: 121 CQDIGFAQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFG   R+ MVL+VAFCVADLFPSLWWID IR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGVTVRRAMVLIVAFCVADLFPSLWWIDIIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DC K LDTF SKV+EEHKEKIRD VGTDESKKDFVDIMLQLQ+D MVDYHFSP
Sbjct: 241 GFNKELKDCSKTLDTFVSKVIEEHKEKIRDGVGTDESKKDFVDIMLQLQQDDMVDYHFSP 300

Query: 301 INLKAIVLVRF 311
            +LKA++L  F
Sbjct: 301 DSLKAMLLDMF 311

BLAST of Csor.00g044810 vs. NCBI nr
Match: XP_023542196.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542 bits (1396), Expect = 1.84e-189
Identity = 271/311 (87.14%), Postives = 287/311 (92.28%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD  TKI QQFQALNPIS SLCLFFFFILLLKL+KRKKPNFPPSPPRLPIIGNLHQLG L
Sbjct: 1   MDLTTKIQQQFQALNPISFSLCLFFFFILLLKLFKRKKPNFPPSPPRLPIIGNLHQLGDL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAA ELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAQELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDI FTQYGEYWRQARKVCALELFS KRVDSFQYVRD+E+GGLMD+I KACVG EAVNL
Sbjct: 121 CQDITFTQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDKIRKACVGGEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENG+SRFGD  R+ MVL+ AFCVAD+FPSLWWID IR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGDSRFGDTVRRAMVLLAAFCVADIFPSLWWIDVIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DC KRLDTFFS+VVEEH+EK+RD V TDESKKDFVDIMLQLQ+D MVDY FS 
Sbjct: 241 GFNKELKDCSKRLDTFFSEVVEEHEEKMRDGVLTDESKKDFVDIMLQLQQDDMVDYPFSL 300

Query: 301 INLKAIVLVRF 311
            +LKAI+L  F
Sbjct: 301 DSLKAILLDMF 311

BLAST of Csor.00g044810 vs. ExPASy TrEMBL
Match: A0A6J1GRU2 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 2.57e-215
Identity = 304/311 (97.75%), Postives = 305/311 (98.07%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MDFPTKIHQQFQALNPISSSLCLFFFFI+LLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDFPTKIHQQFQALNPISSSLCLFFFFIVLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKT DIKFSNRPQTTAAYELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTQDIKFSNRPQTTAAYELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRI KACVGVEAVNL
Sbjct: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIRKACVGVEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNKQLEDCFKRLDTFFSKVVEEHKEKIRD V TDESKKDFVDIMLQLQRDGMVDYHFSP
Sbjct: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDGVLTDESKKDFVDIMLQLQRDGMVDYHFSP 300

Query: 301 INLKAIVLVRF 311
           INLKAIVL  F
Sbjct: 301 INLKAIVLDMF 311

BLAST of Csor.00g044810 vs. ExPASy TrEMBL
Match: A0A6J1GRT5 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=1)

HSP 1 Score: 549 bits (1414), Expect = 1.82e-192
Identity = 272/311 (87.46%), Postives = 287/311 (92.28%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD   KI QQFQALNPIS SLCLFFFF+LLLKL++RKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDLTRKIQQQFQALNPISLSLCLFFFFVLLLKLFERKKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKH LVVSS KMAREVMKTHDIKFSNRPQTTAA+ELLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGF QYGEYWRQARKVCALELFS KRVDSFQYVRD+E+GGLMDRI KACVG EAVNL
Sbjct: 121 CQDIGFAQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFG   R+ MVL+VAFCVADLFPSLWWID IR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGVTVRRAMVLIVAFCVADLFPSLWWIDIIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DC K LDTF SKV+EEHKEKIRD VGTDESKKDFVDIMLQLQ+D MVDYHFSP
Sbjct: 241 GFNKELKDCSKTLDTFVSKVIEEHKEKIRDGVGTDESKKDFVDIMLQLQQDDMVDYHFSP 300

Query: 301 INLKAIVLVRF 311
            +LKA++L  F
Sbjct: 301 DSLKAMLLDMF 311

BLAST of Csor.00g044810 vs. ExPASy TrEMBL
Match: A0A6J1GR89 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456801 PE=3 SV=1)

HSP 1 Score: 529 bits (1363), Expect = 1.43e-184
Identity = 267/311 (85.85%), Postives = 279/311 (89.71%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD    I QQFQALNPIS SLCLFFFFILLLK++KRKKPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 16  MDLTMMIQQQFQALNPISFSLCLFFFFILLLKIFKRKKPNFPPSPPRLPIIGNLHQLGAL 75

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQKH LVVSS KMAREVMKTHDIKFSNRPQTTAAYELLYQ
Sbjct: 76  PHQSMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAYELLYQ 135

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDIGF QYGEYWRQARKVCALELFSAKRVDSF YVRD+E+GGLMDRI KACVG EAVNL
Sbjct: 136 CQDIGFAQYGEYWRQARKVCALELFSAKRVDSFPYVRDEEVGGLMDRIRKACVGGEAVNL 195

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLG KFE EN  SRFGD  +K ++L+ AFCVAD FP LWWIDTIR
Sbjct: 196 SQLFLQTSNNIVSRCVLGGKFEGEN--SRFGDTTKKFLLLLTAFCVADFFPCLWWIDTIR 255

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DCFK LDT FSKVVEEHK KIRD V TDESKKDFVDIMLQLQ+D MVDY FSP
Sbjct: 256 GFNKELKDCFKTLDTIFSKVVEEHKAKIRDGVLTDESKKDFVDIMLQLQQDDMVDYPFSP 315

Query: 301 INLKAIVLVRF 311
            NLKAI+L  F
Sbjct: 316 DNLKAILLDMF 324

BLAST of Csor.00g044810 vs. ExPASy TrEMBL
Match: A0A6J1GTK0 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456989 PE=3 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 5.26e-184
Identity = 262/308 (85.06%), Postives = 279/308 (90.58%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD    I QQFQALNPIS SLCLFFFFI+LLK++KR KPNFPPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDLTMMIQQQFQALNPISFSLCLFFFFIVLLKIFKRNKPNFPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNK+GPLMLLKLGQ   LVVSSAKMAREVMK+HDI FSNRPQTTAA+EL+Y 
Sbjct: 61  PHQSMAALSNKHGPLMLLKLGQTQALVVSSAKMAREVMKSHDIIFSNRPQTTAAHELMYL 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQ+IGF QYGEYWRQARKVCALELFSAKRVDSFQYVRDDE+GGLMDRIGKACVG EAVNL
Sbjct: 121 CQNIGFAQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEVGGLMDRIGKACVGGEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTS+NIVSRCVLG KFE ENGNSRFGD  +K  +L+ AFCVAD FP LWWIDTIR
Sbjct: 181 SQLFLQTSSNIVSRCVLGGKFEGENGNSRFGDTTKKFFLLLTAFCVADFFPCLWWIDTIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GFNK+L+DCFK LDTFFSKVVEEHKEKIRD VGTDESKK FVDIMLQLQ+D MVDY FSP
Sbjct: 241 GFNKELKDCFKALDTFFSKVVEEHKEKIRDGVGTDESKKIFVDIMLQLQQDDMVDYPFSP 300

Query: 301 INLKAIVL 308
            NLKAI+L
Sbjct: 301 DNLKAILL 308

BLAST of Csor.00g044810 vs. ExPASy TrEMBL
Match: A0A6J1JZG2 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490236 PE=3 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 2.68e-179
Identity = 259/311 (83.28%), Postives = 278/311 (89.39%), Query Frame = 0

Query: 1   MDFPTKIHQQFQALNPISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGAL 60
           MD   KI QQFQAL+PIS S+CLFFF I+LL+L+KR KPN PPSPPRLPIIGNLHQLGAL
Sbjct: 1   MDLTRKIQQQFQALDPISFSICLFFFVIVLLRLFKRNKPNLPPSPPRLPIIGNLHQLGAL 60

Query: 61  PHQSMAALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQ 120
           PHQSMAALSNKYGPLMLLKLGQK  LVVSS K+A+EVMKTHDIKFSNR QTTA+ +LLYQ
Sbjct: 61  PHQSMAALSNKYGPLMLLKLGQKQALVVSSPKIAKEVMKTHDIKFSNRLQTTASKKLLYQ 120

Query: 121 CQDIGFTQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNL 180
           CQDI F  YGEYWRQARKVCALELFS KRVDSFQYVRDDE+GGLMDRI KA VG EAVNL
Sbjct: 121 CQDIAFVHYGEYWRQARKVCALELFSVKRVDSFQYVRDDEVGGLMDRIRKASVGGEAVNL 180

Query: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIR 240
           SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGD ARK+MVL+VAFC ADLFPSLWWID IR
Sbjct: 181 SQLFLQTSNNIVSRCVLGEKFEDENGNSRFGDTARKLMVLIVAFCAADLFPSLWWIDIIR 240

Query: 241 GFNKQLEDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSP 300
           GF+K+L+DCFK LDTFFSKV+EEHKEKIR  V TDESK DF+DIMLQLQ+D MVDY+FS 
Sbjct: 241 GFDKELKDCFKTLDTFFSKVLEEHKEKIRSGVLTDESKMDFLDIMLQLQQDDMVDYNFSS 300

Query: 301 INLKAIVLVRF 311
            NLKAIVL  F
Sbjct: 301 DNLKAIVLDMF 311

BLAST of Csor.00g044810 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 122/289 (42.21%), Postives = 182/289 (62.98%), Query Frame = 0

Query: 20  SLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLMLLK 79
           SL +F   +  +K  K KK N P SPPRLP+IGNLHQLG  PH+S+ +LSN+YGPLMLL+
Sbjct: 11  SLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLR 70

Query: 80  LGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQARKV 139
            G    LVVSSA +AR+++KT+D  F++RP++    ++ Y+ +D+    YGEYWRQ + V
Sbjct: 71  FGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSV 130

Query: 140 CALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVLGE 199
           C L L + K V SF+ VR +EI  +M++I K+      VNLS+L    +N+++SR  LG 
Sbjct: 131 CVLHLLTNKMVRSFRNVRQEEISLMMEKIQKS--SSLQVNLSELLGSLTNDVISRVALGR 190

Query: 200 KFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFFSK 259
           K+ DE   + F +  +++  L+  FCV    P L WID I G + QL+     LD F  K
Sbjct: 191 KYSDE---TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEK 250

Query: 260 VVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVL 309
           VV++H++        D  + DFVD++L++QR+  V +    +++KAI+L
Sbjct: 251 VVQDHED-------GDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIIL 287

BLAST of Csor.00g044810 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 125/292 (42.81%), Postives = 179/292 (61.30%), Query Frame = 0

Query: 17  ISSSLCLFFFFILLLKLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLM 76
           I  SL +F   +   K  + KK N P SPPRLP+IGNLHQLG  PH+S+ +LS++YGPLM
Sbjct: 8   ILQSLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHRYGPLM 67

Query: 77  LLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQA 136
           LL LG+   LVVSSA +AR+++KTHD  F++RP++    +L Y  +D+ F  YGEYWRQ 
Sbjct: 68  LLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYWRQI 127

Query: 137 RKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCV 196
           + VC L L S K V SF+ VR +EI  +M++I K+      VN+S+L    +N+++SR  
Sbjct: 128 KSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKS--SSLQVNVSELLGSLTNDVISRIA 187

Query: 197 LGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTF 256
           LG K+  E  +    +  +++M+LM  F V    P L WID I G + QL      LD F
Sbjct: 188 LGRKYSGETDSK---ELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEF 247

Query: 257 FSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVL 309
             KVV++H       V  D  + DFVD++L++QR+  + +    + +KAIVL
Sbjct: 248 LEKVVQDH-------VDGDGQRTDFVDVLLRIQREKSIGFEIDRLCIKAIVL 287

BLAST of Csor.00g044810 vs. TAIR 10
Match: AT2G30770.1 (cytochrome P450, family 71, subfamily A, polypeptide 13 )

HSP 1 Score: 227.3 bits (578), Expect = 1.9e-59
Identity = 121/305 (39.67%), Postives = 181/305 (59.34%), Query Frame = 0

Query: 9   QQFQALNPISSSLCLFFFFILLLKLYKR--KKPNFPPSPPRLPIIGNLHQLGALPHQSMA 68
           Q+ + +  IS  L      +LL +  KR   K N PPSP RLP+IGNLHQL   PH+S+ 
Sbjct: 5   QEMEMILSISLCLTTLITLLLLRRFLKRTATKVNLPPSPWRLPVIGNLHQLSLHPHRSLR 64

Query: 69  ALSNKYGPLMLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGF 128
           +LS +YGPLMLL  G+   LVVSS + A+EV+KTHD KF+NRP++ A + L+   +D+ F
Sbjct: 65  SLSLRYGPLMLLHFGRVPILVVSSGEAAQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVF 124

Query: 129 TQYGEYWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQ 188
             YGEYWRQ + VC L L + K V+SF+ VR+DE+  +++++ KA     + NLS+LF+ 
Sbjct: 125 APYGEYWRQMKSVCILNLLTNKMVESFEKVREDEVNAMIEKLEKASSSSSSENLSELFIT 184

Query: 189 TSNNIVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQL 248
             +++ SR  LG K  ++          R++M L+  F + +  P L WID IRGFN ++
Sbjct: 185 LPSDVTSRVALGRKHSEDETARDLKKRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKI 244

Query: 249 EDCFKRLDTFFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAI 308
           ++  +       KVV+EH E   D       K DFVDI+L +++D    +     ++K +
Sbjct: 245 KEVSRGFSDLMDKVVQEHLEASND-------KADFVDILLSIEKDKNSGFQVQRNDIKFM 302

Query: 309 VLVRF 312
           +L  F
Sbjct: 305 ILDMF 302

BLAST of Csor.00g044810 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 225.7 bits (574), Expect = 5.6e-59
Identity = 123/291 (42.27%), Postives = 181/291 (62.20%), Query Frame = 0

Query: 21  LCLFFFFILLL---KLYKRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPLML 80
           LC   F + ++   K  + KK N  PSPP LP+IGNLHQLG  PH+S+ +LS++YGPLML
Sbjct: 8   LCSIIFVVTIIIFRKQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLML 67

Query: 81  LKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQAR 140
           L  G+   LVVSSA++AR+V+KTHD  F++RP++    +LLY   D+    YGEYWRQ +
Sbjct: 68  LHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMK 127

Query: 141 KVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRCVL 200
            VC L LFS K V SF+ VR++EI  +M++I K+      VNLS++ +  +N+++ +  L
Sbjct: 128 SVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSI--SLPVNLSKILVSLTNDVICKVAL 187

Query: 201 GEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDTFF 260
           G K+    G + F +   ++  L+  F V    P L WID IRG + QLE     +D FF
Sbjct: 188 GRKY---GGETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFF 247

Query: 261 SKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVL 309
            +VV++H +  RD         DFVD++L +QRD  V +  + +++KAIV+
Sbjct: 248 ERVVQDHVDGNRD-------MTDFVDVLLAIQRDKTVGFEINRVSIKAIVM 286

BLAST of Csor.00g044810 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 122/307 (39.74%), Postives = 186/307 (60.59%), Query Frame = 0

Query: 20  SLCLFFFFILLL--KLYKR--KKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPL 79
           SLCL     LLL  K  KR  KK N PPSP R+P+IGNLHQL   PH+S+ +LS +YGPL
Sbjct: 8   SLCLTTLLTLLLLKKFLKRTAKKVNLPPSPWRIPVIGNLHQLSLHPHRSLHSLSLRYGPL 67

Query: 80  MLLKLGQKHTLVVSSAKMAREVMKTHDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQ 139
           MLL  G+   LVVSS++ A E++KTHD+KF+NRP++ A + L+   +D+ F  YGEYWRQ
Sbjct: 68  MLLHFGRVPILVVSSSEAAHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQ 127

Query: 140 ARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIGKACVGVEAVNLSQLFLQTSNNIVSRC 199
            + VC L L + K V SF+ VR++E+  +M+++ KA     A NLS+LF+  ++++ SR 
Sbjct: 128 MKSVCILNLLTNKMVASFEKVREEEVNAMMEKLEKASCSSSAENLSELFVTLTSDVTSRV 187

Query: 200 VLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRGFNKQLEDCFKRLDT 259
            LG+K+ ++          R++M L+  F + D  P+L WID I GFN ++ +  +    
Sbjct: 188 SLGKKYWEDETAGGLKKRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSD 247

Query: 260 FFSKVVEEHKEKIRDDVGTDESKKDFVDIMLQLQRDGMVDYHFSPINLKAIVLVRFFVHL 319
              KVV+EH E         E K DFV+I+L ++++    +     ++K ++L  F   +
Sbjct: 248 LMEKVVQEHLE-------AGEHKADFVNILLSIEKEKNNGFKVQRNDIKFMILDMFIGGI 307

Query: 320 TLKAIVL 323
           +  + +L
Sbjct: 308 STSSTLL 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P244653.5e-7446.69Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q6092.1e-7147.28Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP... [more]
Q9STL12.2e-6042.21Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1[more]
Q9STL23.2e-5942.81Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1[more]
A0A0N9HTU14.1e-5940.00Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 ... [more]
Match NameE-valueIdentityDescription
KAG6573092.17.56e-248100.00Phenylacetaldehyde oxime monooxygenase CYP71AN24, partial [Cucurbita argyrosperm... [more]
XP_022954772.15.31e-21597.75cytochrome P450 71A1-like [Cucurbita moschata][more]
XP_023542640.13.38e-20291.64cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo][more]
XP_022954771.13.75e-19287.46cytochrome P450 71A1-like [Cucurbita moschata][more]
XP_023542196.11.84e-18987.14cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GRU22.57e-21597.75cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=... [more]
A0A6J1GRT51.82e-19287.46cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=... [more]
A0A6J1GR891.43e-18485.85cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456801 PE=3 SV=... [more]
A0A6J1GTK05.26e-18485.06cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456989 PE=3 SV=... [more]
A0A6J1JZG22.68e-17983.28cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490236 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48310.11.6e-6142.21cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
AT3G48320.12.3e-6042.81cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT2G30770.11.9e-5939.67cytochrome P450, family 71, subfamily A, polypeptide 13 [more]
AT3G48270.15.6e-5942.27cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT1G11610.11.2e-5839.74cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 187..205
score: 20.55
coord: 69..88
score: 32.87
coord: 93..114
score: 24.23
IPR001128Cytochrome P450PFAMPF00067p450coord: 42..311
e-value: 5.0E-35
score: 121.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 29..316
e-value: 6.3E-53
score: 182.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 40..312
NoneNo IPR availablePANTHERPTHR47955CYTOCHROME P450 FAMILY 71 PROTEINcoord: 23..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g044810.m01Csor.00g044810.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen