Csor.00g017340 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g017340
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPseudoU_synth_1 domain-containing protein
LocationCsor_Chr13: 163127 .. 168014 (+)
RNA-Seq ExpressionCsor.00g017340
SyntenyCsor.00g017340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAACCAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATCGGCTATGTTGGGACTGATTATAGAGGTCTCATTTGATATTACTCTCTGGTTCTCTTGACGACCTTTTTGTGAATCCTTGACCAGTTCATTAATTCTTGAGTTTAAAAAATGCAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAAGTGAGCTTTTTTCATCATTTCTTTTTACATCATTTACATAATGTCATGAAAATTACTGCGGGGATTGGCAATGACATCCGCCGGATATTCTGTTGCTGTCTTTGATTTGCATACTTTCGTAGTGAATGTGATGGTGGTTGCTCAATTACTTACTTAGCCAAGTGTAAAGTACAACAACTATTTACAACAATTTAGTTCACAGTGACTAACAGAAACAGTTGAAATTGACTGACCAATTGTGATATGAACCAAGACATCGATCCTGCAATATAAACTTCAAGAAAGATGTGAAGAAAATGTTATTCAAATTATCACAAGATGTTGCAATTCATACATATTAAAGAAGAATGAAGCTTCATTTATTCTTAGATTGTTACAAATTTTGAGTGGATGATAATTTAGAGTTACGGTATTTCACTAATGTACTTAAAGACTTTTTATTTACTCTAAGGTTACATTTACATGGCTAATTATGACCATAAAGTATACATAGTGTTCATAAGATATGAAAGTTACAACTCTTGGAATGTCTCATAGAAGGTTATGGGTTTTCATTTTGAGACTATATAAAGCTATGGATGTTATCTTTGTAAGGGAGATTTGGAAAATGCAGTAAAAACAGCATTTTTAGGGCTAATTTTCTTTGCAATTCTATGTAGTTTAGGTTGCATTTTAGAATCATTCAAGCGTGATCCGAATCTCAAACAAGTGTTCTTGCCTTGAGATATTCGATCAACAAGGTCATCCGAATCTTACTTCCCTTGTGGTGATTCCTTATCCTTTTGTATCAAAAGCCTTATCAAATTAACTATTGATAGCTAATTAAATAGTCAATTTCTCTACTCTAAAGCCATGAATATCATTTTCTTAAGTGTTCTAAAGGTGATGGATGTTTGAAAGACTCATTGATAAAGCATTCATGGTTCAAGCTATCTATTGCAACTAGCGCTCGAAACCTTGGAACTTATTATCAGACGTTCCTATGATGTACTGACAGATTGGATTCCATGTATTTCAGACAATCTAAGAGGCAAGTTTTCAAGGGGAACTTTGTAACATGCTTGCTGTTTCATGATTTGGAGAACTAGAGATGCGTGGCAGAATAGTTACTGCTAAAAGAGTACATAGAAAGGACATGTATCAGCAACACTAATGCCATTAAACTTTCAGCGATAATGAAGTGATACTCGCACCATGCATTAGTTACTAACTACAACAAATTTAAAATATAACTGCAGACTGAACCAACAGAATAGTTGAGGTTAGTATGCATTCAGCTGATCAATATCTGATTAAATACACTTAACTTCTTAACCAACCTTGATATATTGTTTTATGTACATTAATCTCATAGGATCTGTCGTAGACTTCCATTTATTTTATTGGATAAGCATTTCTTTGTCTTTCAATGTTTCTTTTACCATTAAACAATGTTAAGTGCCCATGTTGGTTATGATAATAAGAAATTAACCTCGTACAGTTCAAAGTACCCAGCTAATGCATGGATGGAATGTCCTAAAGCATTACAGTTTTAATGTGTAGCAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTAAATATCCTGAATGTCTTTGTTGCATCTCATTTTATGACTTTTGTAATCTTATTGGTTGTTTTGATTTGTTTTTTCTTTCTCTTTCATTTTCAAGGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGTAAGTGCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGCTTTGAAGTGAGTAAATGAAGTTTCAGCGTTAGCATGATGCGTTTGTATGTTTTTACTACAATGTGGTAACTTTACATCATTTTTTTCACTTGTCAAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCCTTTCAGGTAGGTTTTTATCACCTTCATTTAATTACATAAAGATCAGTTTGTACCTCCCTTCACTGCAAGAAACATGATTTGTTATTATTTGATAGTCTAGTACTGTTATTTGGAAGACAATTGTATGGCGTTGAAGAACATAGGTGGATTCTTTTTGCGTACAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAGCCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGGTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATTCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCGCACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAGTTAGTCTTTCTCTAACTTTGTTTTCTTTGGTGCATAATTTGTTCTTATTTCCGTTGTCCATGTCTACTTCATTTATTCTTATTTCCGTTGGCAGCTCTATCGCTTAGATTTGGGTGGTTTTCCGTTCTAACACCTCTTGTAGCTTAGGTCATGATTCTGGTCACGTGGGAGCCAAAACTTAGTATATAACTGTTCTTTTGTCCCCCACTCGATAATTTCTTTGCAGCCTTATTTCATTTTTGAACTATTTTGCCCCTTTGGAAATCAATGCTCAGTGGCCTAGTGGGGTTTTTCTGTGCTAGTAGTCCTTCAAATAAAATTAAGAAATAGTCTTTATAAGCTTGACTTGTTCAATATCTATCATCATAACCTTCATTTACTCCCCAAGGTTCATAACTCATAGGATCATCTTACACCATTAGACATGTCTGGTTTAATTTTTTTGCACTTGACCCATTCGTATTCAAAATCAATTCGCATGAAATTAAATTCTAAGATGATAGGAACCAGTTAATAATTTGATGGTCCCTGAGCATTGATGGACAGAAAGCTTTATGAGCTTGACCTGTTCAATATCTACCATCATTTTACTCCCTGAGGTTTATAATTCATAGGAATAGCTCACAAGACATGTCTAGGTTAACTTTTCTGCACTTGACTATTGTTATTCAAAACTAATTCTTATTATCTGGATAGAAGATGGAAGTAGAATCTCTATCCAGACAAGAGAAAGTAGAATTTGTTTGAAAAGCTTTTGCTTCTCTGTTAGAGATTAAACCAGGTTAGGAATCACTGTACAATGATATGATTTTGTCCCCTTTGAGCATAAGCTCTCATAGCTTCGCTTTGGACGTCTCCATATGGTCTCATACCAATAGAGATATATTTCTTACTTATAAACTCATGATCGAGCCTAAATTAGCCAATGTGGGACTCCCTCCAAACAATCCTCAACAAACCATTCCGTTGAGGTCAAAGCCTCAAGAAGAAACTCAGAATCTTATGAACAAATGGCGTATGGAGAGGCACTAACATGAGCCATAAAGTTATCTACTCTTATCTTGAGGTGGAGGTTCGAACCCACACTTCCACATTTATCGTACTCAAAAGTGGTAAGAAATGGAAGGCATTATTGCAGGTTTAGGACTTGGAAGGAGATATGGGAAGTGGCAAGGTGGCGTCCTTTTTATACTTTTCGAGTCGTTATGTGATACTTTAAGGATGAAGTTGGCTCCATCAGAAATTAGTATTTGGTAGTTAATGTTGTACTTTTAACTCAAAGGGCTATTCTCATGCTGCTTTTATTACACCCTGGGCAGATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGAAAACTACTCCCAAGAGATATCATAGCATTGTCGCTGAGCAAGTTCTCTAGAATAGTTATGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

mRNA sequence

ATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAACCAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATCGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAACAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAGCCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGGTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATTCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCGCACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGAAAACTACTCCCAAGAGATATCATAGCATTGTCGCTGAGCAAGTTCTCTAGAATAGTTATGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

Coding sequence (CDS)

ATGGCAGTCTCTTCTCTGCGATTCCCGCTTTCCCCATGGGCAACCAAAACTTCATTTCCATGCTTCCGTTTTCCCTACCCTAATTTCGTTTCGTGGACCTCTCGTCTGAGTCCTAGACTGCTGTGCTGTTCCTCATCTTCTACTGCTTCCACCGATGCTCTTTCCACTCAGTGCGCCCCAGACCAGCTCCGGAAATGGGAAGCTTTTCGGAAGAAGAAGGTCGTTATGCGGATCGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAATCATCACATCCAACAATTGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGATAGTGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATCAACTTTTATCTTCCTGAAAATATCAGGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAGGGAGTGTGATGTTCGGAAGTATACTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAGCTCACAACTACTGAAATTGATTATCACATCTCAGAATTTAAGGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGGATTCGAGCCAACCAATCTCCAGGATATGTCCACAAGTCAGGAGATGGGGTATCATCTAGTGATTTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAAACGATATTTTTGGTGGAGGAATCAAGGAACCAAATCAACATTCTCCAAGCTTAAGTGGTAGTTATGAGAATACATTTGAAGTATTCAGTAAACTTGGAAATGGATCAGAGCTTCCACAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCGCACTTCAGAAAAATCCTATACTGTTCTTGTGGGAAGCTGGAAACATCTCTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCTTTCATGTTGCATCAAATTCGAAAGATGGTAGGCACAGCCATTGCCGTGAAGCGAAAACTACTCCCAAGAGATATCATAGCATTGTCGCTGAGCAAGTTCTCTAGAATAGTTATGCCCATTGCTCCAGCTGAAGTCTTAGTCCTAAGAGGGAACAGGTTTTCCAACAGAAAACGACCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTAAACGAATCCCACGACATTGTCAAGTCAGTGAATGAATTCTACAGATCGGCGATATTACCCGAGGTAACTAACTTTCTAGACATAGAAAGACCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGAGGAAGTTAGGAATGCTTGGAAGGCATGGACAGGAAAAGGTAGATTGTCTTGA

Protein sequence

MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAPDQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Homology
BLAST of Csor.00g017340 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.2e-141
Identity = 266/491 (54.18%), Postives = 352/491 (71.69%), Query Frame = 0

Query: 23  RFPYPNFVSW----TSRLSPRLLCCSSSST--ASTDALSTQCAPDQLR-KWEAFRKKKVV 82
           R P P+ V W      R++P LL  SSS+    S+ +LS   + + L  KWE++RKKKVV
Sbjct: 7   RIPIPSRVFWYPTRLIRVTPALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVV 66

Query: 83  MRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDK 142
           +RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+SNYG+L+KI WARSSRTDK
Sbjct: 67  IRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDK 126

Query: 143 GVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECD 202
           GVHSLAT+I+LKMEIP++AW  DP G +LA  I+ +LPENIRVF ++PS R FDPRREC 
Sbjct: 127 GVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECT 186

Query: 203 VRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRI--R 262
           +RKY+YLLP  ++GI++  T+ EIDYHI++F +IL  F+GE+PFHNYT RS+YR +   +
Sbjct: 187 LRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK 246

Query: 263 ANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENTFEVF 322
             Q  G   +    + +S+   SEF E   EN I  G  +E  +    + G  E+   V 
Sbjct: 247 IKQRNGRPPREPKSIKASE---SEFRE---ENHIEIGEEEEEKE----VDG--ESDEHVV 306

Query: 323 SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGES 382
           +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE SLG+ FVEISIWGES
Sbjct: 307 TPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGES 366

Query: 383 FMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVLRGNRFSNRKRPG 442
           FMLHQIRKM+GTA+AVKR+LLPRDII LSL+KF+RIV+P+AP+EVL+LRGN F  R+   
Sbjct: 367 FMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR--- 426

Query: 443 APKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQ 502
            P+RP +    ES ++ K + EFYR+ ++P+V+ FLD E+ PWKEWV+ LD+   + +E+
Sbjct: 427 LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEE 480

Query: 503 LEEVRNAWKAW 505
           LE+VR  W+ W
Sbjct: 487 LEDVRKGWEEW 480

BLAST of Csor.00g017340 vs. ExPASy Swiss-Prot
Match: Q6FV05 (tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.2e-23
Identity = 119/438 (27.17%), Postives = 191/438 (43.61%), Query Frame = 0

Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV + IGY GT Y G+Q       +PTIE  L  A   AG I ++N  +L K ++ R+
Sbjct: 86  KRKVAVMIGYCGTGYHGMQY---NPPNPTIEAALFKAFVDAGAISKANSNDLKKNNFMRA 145

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I D   M         + IN  LPE IR++ I    ++FD 
Sbjct: 146 ARTDKGVHAGGNLISLKMIIEDPEIM---------DKINANLPEGIRIWDIERVNKAFDC 205

Query: 191 RRECDVRKYTYLLPA-KLIGIE-SKLTTTEIDYHISEFKDILN-SFQGEHPFHNYTIRSK 250
           R+ C  R Y YLLP   LIG +   +   +I+   +E   +L+   + +  + N+   +K
Sbjct: 206 RKMCSSRWYEYLLPTYSLIGPKPGTILNNDIESSKTELPGVLDEDLESKEFWENFEAAAK 265

Query: 251 YRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYE 310
                    +P  +       +  D S    EE D + +++     +  +    L  ++ 
Sbjct: 266 ------KEFTPEEI------AAIKDYSPPPREEFDDQEELY-----QKVKQWKLLENTHR 325

Query: 311 NTFEVFSKLGN--GSELPQGVRARWLHE----PDEKDRIGASHFRKILYCSCGKLETSLG 370
             + +     N   + + Q + A   H      + KD   A  F K +  S   +     
Sbjct: 326 RQYRISEMKLNKFRAAMNQYLGAHNFHNFTLGKEFKDP-SAIRFMKEIKVSDPFVIGDAK 385

Query: 371 YDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVL 430
            ++V I I G+SFMLHQIRKM+  A  + R   P + I+ +     +I +P APA  L+L
Sbjct: 386 AEWVSIKIHGQSFMLHQIRKMISMATLIARCGTPVERISQAFGP-QKINIPKAPALGLLL 445

Query: 431 RG---NRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKE 490
                  ++ R      K  D     E  D  K +   Y      EV++ +      +  
Sbjct: 446 EAPVFESYNKRLEKFGYKPIDFSKYQEKVDAFK-MKHIYDKIYAEEVSDNV------FNA 485

Query: 491 WVEKLDKYTSIPNEQLEE 497
           +   +D +  +   Q EE
Sbjct: 506 FFSYIDSFNKVTGAQTEE 485

BLAST of Csor.00g017340 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 5.5e-23
Identity = 111/371 (29.92%), Postives = 170/371 (45.82%), Query Frame = 0

Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV + +GY GT Y G+Q+Q + +   TIE+++  A+  AG I   N  +L K  + R+
Sbjct: 148 KRKVAVMLGYCGTGYNGMQVQNDPNV-KTIEKDIYDAMATAGAISAENAVDLKKSGFQRA 207

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I D           + N IN  LP+ IR++GI  + + FD 
Sbjct: 208 ARTDKGVHAAGNVISLKMIIEDPE---------IINKINDLLPKQIRIWGIQRTTKGFDC 267

Query: 191 RRECDVRKYTYLLPA-KLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYR 250
           R+ C  R Y YLLP   L+  + K   +E+   + E K + N    E            +
Sbjct: 268 RKCCSSRIYEYLLPTFSLLPPKPKSVLSEL---VKE-KKLENPDLFEDDQEGIDWWENVK 327

Query: 251 TRIRANQSPGYVHKSGDGVSSS-----------DLSNSEFEESDGENDIFGGGIKEPNQH 310
           ++I A+   G   +  D ++SS               ++ EE D     F   IK+    
Sbjct: 328 SKILAS---GITQEQIDSITSSYDEQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTI 387

Query: 311 SPSLSGSY---ENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGK 370
                 SY    +  + F ++    E         + +P  KD        K +      
Sbjct: 388 ENQSRRSYRISSSRLQHFREVMKQYEGTHNFHNFTVGKP-FKDTSANRFMIKTIVSDPFV 447

Query: 371 LETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAP 427
           +E   G +++ I I G+SFMLHQIRKM+  A  V R  LP  II  +  + ++I +P AP
Sbjct: 448 IE---GTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIIN-NFFQSTKINIPKAP 496

BLAST of Csor.00g017340 vs. ExPASy Swiss-Prot
Match: Q6CWQ8 (tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.6e-22
Identity = 109/358 (30.45%), Postives = 163/358 (45.53%), Query Frame = 0

Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV + IGY GT Y G+Q       + TIE+EL  A  +AG I ++N  +L K  + R+
Sbjct: 98  KRKVAVMIGYCGTGYHGMQY---NPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRA 157

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LK+ I D           +   IN  LP+ IRV+ I    ++FD 
Sbjct: 158 ARTDKGVHAGGNVISLKLIIEDPE---------VKEKINNELPDQIRVWDISRVNKAFDC 217

Query: 191 RRECDVRKYTYLLPA-KLIGIE-SKLTTTEIDYHISEFKDILNSFQGEHPF---HNYTIR 250
           R+ C  R Y YLLP   LIG + S     EI+    E  +++        F    +    
Sbjct: 218 RKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIEASKKEIPNVIQDDVESAQFWEAFSTEAE 277

Query: 251 SKYRTRIRANQSPGYV--HKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLS 310
           SK+ T+    +   +V    S D  + +   +  F++ + E+       KE      S  
Sbjct: 278 SKF-TKEELEEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSAL 337

Query: 311 GSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYD 370
             YE TF  F     G +          ++P       A  F K +  S   +      +
Sbjct: 338 KQYEGTFN-FHNFTLGKD---------FNDP------SAKRFMKQITVSEPFVIGEAKTE 397

Query: 371 FVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVL 422
           +V I I G+SFMLHQIRKM+  A  + R   P + I  +  +  +I +P APA  L+L
Sbjct: 398 WVSIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLL 425

BLAST of Csor.00g017340 vs. ExPASy Swiss-Prot
Match: Q755C8 (tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 1.4e-21
Identity = 106/358 (29.61%), Postives = 161/358 (44.97%), Query Frame = 0

Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV + +GY GT Y G+Q       + TIE EL  A  KAG I  +N  +L K  + R+
Sbjct: 66  KRKVAVMVGYCGTGYHGMQY---NPPNRTIEAELFEAFVKAGAISRANSTDLKKNGFMRA 125

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LK+ I D A         + + IN +LP  IRV+GI    ++FD 
Sbjct: 126 ARTDKGVHAGGNVISLKLIIEDPA---------IKDKINEHLPPGIRVWGISRVNKAFDC 185

Query: 191 RRECDVRKYTYLLPA-KLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYR 250
           R+ C  R Y YLLP    IG        + + +++   +       E P  +      + 
Sbjct: 186 RKLCGSRWYEYLLPTYSFIG-------PKPNTYLARTIEQCGEAASEKPDRDQESLDFWE 245

Query: 251 TRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENT 310
              +A        +    V+    S  +F+E+         G+ +  +    +  ++  +
Sbjct: 246 AFRKAVDEKFTQEEQDAIVNYVAPSKEDFDEN--------SGLYQKVKQYKQMENAHRRS 305

Query: 311 FEVFS-KLGNGSE-LPQGVRARWLHE----PDEKDRIGASHFRKILYCSCGKLETSLGYD 370
           + V S KL    E + Q +     H      D KD      F K +  S   +   +  +
Sbjct: 306 YRVSSAKLARFREAMKQYLGPHNFHNYTLGKDFKDP-STVRFMKDITVSDPFVIGEMKTE 365

Query: 371 FVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVL 422
           +V I I G+SFMLHQIRKM+  A  V R     + IA S     +I +P APA  L+L
Sbjct: 366 WVSIKIHGQSFMLHQIRKMISMATLVARCNCSPERIAQSYGP-QKINIPKAPALGLLL 394

BLAST of Csor.00g017340 vs. NCBI nr
Match: KAG6583326.1 (putative tRNA pseudouridine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1037 bits (2682), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

BLAST of Csor.00g017340 vs. NCBI nr
Match: XP_022964935.1 (putative tRNA pseudouridine synthase [Cucurbita moschata])

HSP 1 Score: 1024 bits (2648), Expect = 0.0
Identity = 505/511 (98.83%), Postives = 507/511 (99.22%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF GGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEV SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

BLAST of Csor.00g017340 vs. NCBI nr
Match: XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 502/511 (98.24%), Postives = 504/511 (98.63%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWATKTSFPCFRFPYPNFV WTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 18  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 78  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSD SNSEFEESDGENDIF GGIKEPNQHSP
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEV SKLGNGSELPQGVRARWLHEPDEKDRIGA HFRKILYCSCGKLETS 
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSL+KFSRIVMPIAPAEVLV
Sbjct: 378 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLTKFSRIVMPIAPAEVLV 437

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 438 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 497

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 498 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 528

BLAST of Csor.00g017340 vs. NCBI nr
Match: XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])

HSP 1 Score: 1010 bits (2612), Expect = 0.0
Identity = 501/512 (97.85%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF-GGGIKEPNQHS 300
           NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF GGGIKEPNQHS
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLSG  ENTFEV SKLGNGSELPQGVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIV+PIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480
           VLRGNRFSNRKRPGAPKRPDLLTLN+S DIVKSVNEFYRSAILPEVTNFLDIERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512

BLAST of Csor.00g017340 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 437/504 (86.71%), Postives = 463/504 (91.87%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLR PLSPW TKTS  CF FPYP FVS T RL P L  CS+SSTASTDA STQCAP
Sbjct: 1   MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           +QLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTI LKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQ+SFDPR+ECDVRKY+Y+LPAKLIGI +KL TTE+DYHIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYRTRIRA +SPGY H+    VS SD+SNSEFEESDGE+D F  G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRAKRSPGYGHE----VSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLS  YEN  EV SKLG+GSELP+ VRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKM+GTAIAVKRKLLPRD+IALSLSKFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPRDVIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRSAILPEVTNFLD ERPPWKEWV
Sbjct: 421 LRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPEVTNFLDTERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAW 504
           EKLDKYTSIP+EQL+EVRNAWKAW
Sbjct: 481 EKLDKYTSIPSEQLDEVRNAWKAW 500

BLAST of Csor.00g017340 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 1024 bits (2648), Expect = 0.0
Identity = 505/511 (98.83%), Postives = 507/511 (99.22%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWA KTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYRTRIR NQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF GGIKEPNQHSP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLSGSYENTFEV SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511

BLAST of Csor.00g017340 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 1010 bits (2612), Expect = 0.0
Identity = 501/512 (97.85%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRL PRLLCCSSSSTASTDALSTQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF-GGGIKEPNQHS 300
           NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIF GGGIKEPNQHS
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLSG  ENTFEV SKLGNGSELPQGVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIV+PIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480
           VLRGNRFSNRKRPGAPKRPDLLTLN+S DIVKSVNEFYRSAILPEVTNFLDIERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 511
           VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAWTGKGRLS 512

BLAST of Csor.00g017340 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 859 bits (2219), Expect = 6.50e-312
Identity = 428/507 (84.42%), Postives = 457/507 (90.14%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPW TKTS  CFR PY  FVS +  L PRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NI+VFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYR  IRA QSPGY H SG+ VS S +SNS FEESD E++ F  G+K PNQH+P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
            LS   E+  EV SKLGNGSEL +GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETSL
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGK 507
           EKLDKYTSIPNEQL+EVRNAWKAW  K
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAWKEK 506

BLAST of Csor.00g017340 vs. ExPASy TrEMBL
Match: A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 859 bits (2219), Expect = 9.46e-312
Identity = 428/507 (84.42%), Postives = 457/507 (90.14%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           MAVSSLRFPLSPW TKTS  CFR PY  FVS +  L PRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NI+VFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRSKYR  IRA QSPGY H SG+ VS S +SNS FEESD E++ F  G+K PNQH+P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
            LS   E+  EV SKLGNGSEL +GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETSL
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIV+PIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAWTGK 507
           EKLDKYTSIPNEQL+EVRNAWKAW  K
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAWKEK 506

BLAST of Csor.00g017340 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 845 bits (2183), Expect = 1.46e-306
Identity = 420/504 (83.33%), Postives = 453/504 (89.88%), Query Frame = 0

Query: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
           M+VSSLR PLSPW TK+S  CFRFPYP FV  T RL P LL C SS+ ASTDA STQCAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVV+RIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LA+YIN YLP+NI+VFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTEID HIS+FKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSP 300
           NYTIRS YRTRIRA QSPGY  KSG+ VS  ++SN EFEESD E+D F  G+KEP+Q SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSGSYENTFEVFSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
           SLS   EN  EV SKL +GSEL +GVRARWLHEPDEKDRIGASHFR+IL CSCGKLETS 
Sbjct: 301 SLS-VCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRD+IALSL+KFSRIV+PIAPAEVLV
Sbjct: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480
           LRGN FS RKRPG P RPDL+TLNES DI+KSVNEFYRSAILPEVT FLD E PPWKEWV
Sbjct: 421 LRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPEVTEFLDTESPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLEEVRNAWKAW 504
           EKLDKYTSIPNE+L+EVR+AWKAW
Sbjct: 481 EKLDKYTSIPNEELDEVRSAWKAW 503

BLAST of Csor.00g017340 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 266/491 (54.18%), Postives = 352/491 (71.69%), Query Frame = 0

Query: 23  RFPYPNFVSW----TSRLSPRLLCCSSSST--ASTDALSTQCAPDQLR-KWEAFRKKKVV 82
           R P P+ V W      R++P LL  SSS+    S+ +LS   + + L  KWE++RKKKVV
Sbjct: 7   RIPIPSRVFWYPTRLIRVTPALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVV 66

Query: 83  MRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDK 142
           +RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+SNYG+L+KI WARSSRTDK
Sbjct: 67  IRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDK 126

Query: 143 GVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECD 202
           GVHSLAT+I+LKMEIP++AW  DP G +LA  I+ +LPENIRVF ++PS R FDPRREC 
Sbjct: 127 GVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECT 186

Query: 203 VRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRI--R 262
           +RKY+YLLP  ++GI++  T+ EIDYHI++F +IL  F+GE+PFHNYT RS+YR +   +
Sbjct: 187 LRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK 246

Query: 263 ANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENTFEVF 322
             Q  G   +    + +S+   SEF E   EN I  G  +E  +    + G  E+   V 
Sbjct: 247 IKQRNGRPPREPKSIKASE---SEFRE---ENHIEIGEEEEEKE----VDG--ESDEHVV 306

Query: 323 SKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGES 382
           +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE SLG+ FVEISIWGES
Sbjct: 307 TPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGES 366

Query: 383 FMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVLRGNRFSNRKRPG 442
           FMLHQIRKM+GTA+AVKR+LLPRDII LSL+KF+RIV+P+AP+EVL+LRGN F  R+   
Sbjct: 367 FMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR--- 426

Query: 443 APKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQ 502
            P+RP +    ES ++ K + EFYR+ ++P+V+ FLD E+ PWKEWV+ LD+   + +E+
Sbjct: 427 LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEE 480

Query: 503 LEEVRNAWKAW 505
           LE+VR  W+ W
Sbjct: 487 LEDVRKGWEEW 480

BLAST of Csor.00g017340 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 116.3 bits (290), Expect = 7.2e-26
Identity = 110/416 (26.44%), Postives = 185/416 (44.47%), Query Frame = 0

Query: 39  RLLCCSSSSTASTDALSTQCAPDQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHP 98
           +L   ++     + A++T  +    R+   +R++KV +   + G  Y+G+Q   +     
Sbjct: 25  KLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAK 84

Query: 99  TIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGD 158
           TIE ELE A++ AG + +++        WARS+RTDKGV ++   ++ +  +       D
Sbjct: 85  TIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYV-------D 144

Query: 159 PCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPA----KLIGIESKL 218
           P G +    +N  LP+ IRVFG      SF  ++ CD R+Y YL+P       +  E+++
Sbjct: 145 PPGFV--ERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEM 204

Query: 219 TTTEIDYHISEFKDILNSFQG-EHPF----HNYTIRSKYRTRIRANQSPGYVHKSGDGVS 278
             T++ Y     K +  S +G + P      +          I+++ S       G  V 
Sbjct: 205 VRTDLGYEY--VKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVK 264

Query: 279 SSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENTF-EVFSKLGNGSELPQGVRA 338
              LS+S     +  N     G       S +      NT  +V    G G ++ +   +
Sbjct: 265 CETLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLAKVEMNNGEGEDMTKS-ES 324

Query: 339 RWLHEPDEKDR--------IGASHF---------------RKILYCSCGKLETSLGYDFV 398
           ++ +   EK+R        +G+ +F               R IL  +   +    G DFV
Sbjct: 325 KFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFV 384

Query: 399 EISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVMPIAPAEVLVL 422
           +  + G+SFMLHQIRKM+G A+A+ R   P  +I  S  K  RI +P+AP   L L
Sbjct: 385 KCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLYL 425

BLAST of Csor.00g017340 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 107.8 bits (268), Expect = 2.5e-23
Identity = 100/385 (25.97%), Postives = 170/385 (44.16%), Query Frame = 0

Query: 72  KKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G +  N +N  LP  IR+FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 RECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYT--IRSKYR 251
           + CD R+Y YLLP   +   S      +   +   ++ +  F+           +  K++
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWK 201

Query: 252 TRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENT 311
                     +  KS D  S    +NS    SD + +     +         +    E++
Sbjct: 202 -------GTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDS 261

Query: 312 FEVFSKLGNGSELPQGVRARWLHEPDEKDR--------IGASHF---------------R 371
            ++ +   N SE    V++++ +   EK+R        +G+ +F               R
Sbjct: 262 CKLNT---NSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANR 321

Query: 372 KILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKF 431
           +I+  +   +    G DF++  + G+SFMLHQIRKM+G A+A+ R      +I  + SK 
Sbjct: 322 QIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSKD 381

BLAST of Csor.00g017340 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 100.1 bits (248), Expect = 5.3e-21
Identity = 92/359 (25.63%), Postives = 158/359 (44.01%), Query Frame = 0

Query: 72  KKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G +  N +N  LP  IR+FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 RECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYT--IRSKYR 251
           + CD R+Y YLLP   +   S      +   +   ++ +  F+           +  K++
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWK 201

Query: 252 TRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFGGGIKEPNQHSPSLSGSYENT 311
                     +  KS D  S    +NS    SD + +     +         +    E++
Sbjct: 202 -------GTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDS 261

Query: 312 FEVFSKLGNGSELPQGVRARWLHEPDEKDR--------IGASHF---------------R 371
            ++ +   N SE    V++++ +   EK+R        +G+ +F               R
Sbjct: 262 CKLNT---NSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANR 321

Query: 372 KILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSK 406
           +I+  +   +    G DF++  + G+SFMLHQIRKM+G A+A+ R      +I  + SK
Sbjct: 322 QIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSK 356

BLAST of Csor.00g017340 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 2.2e-06
Identity = 46/183 (25.14%), Postives = 71/183 (38.80%), Query Frame = 0

Query: 71  KKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+ V ++I Y G  + G       S+   +E  +E+ ++KA        G+     ++R 
Sbjct: 89  KRYVALKIMYFGKRFYGF------SAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRC 148

Query: 131 SRTDKGVHSLATTITLKMEIPDSAWMGDPCGIL-----------LANYINFYLPENIRVF 190
            RTDKGV S    I L +     +  GD    +               +N  LP++IRV 
Sbjct: 149 GRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVI 208

Query: 191 GIVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPF 243
           G  P+   F  R  C  R+Y Y            L  + +D+           F GEH F
Sbjct: 209 GWSPAPIDFHARFSCYAREYKYFF------WRQNLNLSAMDF-------AGKKFIGEHDF 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O229282.2e-14154.18Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032... [more]
Q6FV054.2e-2327.17tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / ... [more]
Q59S635.5e-2329.92tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876... [more]
Q6CWQ83.6e-2230.45tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 23... [more]
Q755C81.4e-2129.61tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... [more]
Match NameE-valueIdentityDescription
KAG6583326.10.0100.00putative tRNA pseudouridine synthase, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022964935.10.098.83putative tRNA pseudouridine synthase [Cucurbita moschata][more]
XP_023520301.10.098.24putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo][more]
XP_022970646.10.097.85putative tRNA pseudouridine synthase [Cucurbita maxima][more]
XP_038894774.10.086.71putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HM830.098.83putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1I1560.097.85putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569... [more]
A0A6J1DMV66.50e-31284.42putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DQ079.46e-31284.42putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A1S3C5W11.46e-30683.33putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=... [more]
Match NameE-valueIdentityDescription
AT2G30320.11.6e-14254.18Pseudouridine synthase family protein [more]
AT1G20370.17.2e-2626.44Pseudouridine synthase family protein [more]
AT1G76120.12.5e-2325.97Pseudouridine synthase family protein [more]
AT1G76120.25.3e-2125.63Pseudouridine synthase family protein [more]
AT1G34150.12.2e-0625.14Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 343..422
e-value: 5.1E-7
score: 30.1
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 63..194
e-value: 2.1E-31
score: 110.5
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 195..495
e-value: 2.2E-34
score: 120.7
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 124..251
e-value: 1.7E-15
score: 55.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..302
NoneNo IPR availablePANTHERPTHR11142:SF21TRNA PSEUDOURIDINE SYNTHASEcoord: 41..505
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 41..505
IPR041708Pseudouridine synthase PUS1/ PUS2-likeCDDcd02568PseudoU_synth_PUS1_PUS2coord: 76..426
e-value: 1.50064E-78
score: 244.445
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 342..428
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 71..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g017340.m01Csor.00g017340.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding