Csor.00g011440 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g011440
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCsor_Chr02: 4933000 .. 4934853 (+)
RNA-Seq ExpressionCsor.00g011440
SyntenyCsor.00g011440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGATATGAATCAGGCGAATGATACTGTGAGAGCTCTCGCGTCGTTGAAGGAAGCTCTGGATTCGACCAAGGAGTTGCTGAGACTTGGGAGCGAGGGGAGCAAGAATGTCTGGTGCGTTTACTCCATTCCATTGTTTGTGTGCTATCTGTGTAATTAGCGCGGTCTGTTCTGCCTTGTAATGTTGTGAATTGATGAATTTCGTTCCGATTCAGTGTTGAATCTTTAATTGTTTCGGATATCTATTTGGCTAAGTGGAAAATGAAAAAAAATGATCGCAGATTATCGGCTATTGTGATTTTCAGACCCTATTTTCGCTTCCATTTGTATCTGCAACAAAACATTTTGCTTAGGCTATAAACAATGTTTCGATGGACATCTTCCGGTTCAACCACACTTTGCTGATGCCTTCCATTCGATTAGACGACGTTCGCATTGCAGCTGTTATCTCGTACTTGCACCTTGTATTAATTTACGTATGATGCTGATTACAATGTGATTCACGATTTCTCTAGATTTTACTCGTATGTACCCTCAACTTGGAATTTTGGTTTTGTGTGAGATGATATGAATTAGGTTCTGTCTAAATTTCTGCCTTTATTCTCAATTGATCATCATGATTTTGCTGAAGATTGGGAAAATGTATGTGAGACAAATCTCGGCAGCCGGTCAGATTATTTCTCCTTTTATCCTCTATATTTTTTTTTTGTTCTAATCTGTTTGTTGATTCGAACTTCAGGCCTTGGAGAAAGAGCAAAATTTGAATCAATTTCACAGGATGACAGCAAGACTAGAGAAAGCTCTGAATGAAATTTCACATGAAGATCTTGATATATCTGATGAAGTTAAGGAGAAGGTAAGGAATTTTATCATTGCGCTTTTTGTTTTTAATATCCTTGAAATGGTTTTCTAGCTGCTCTCGACTGTTGAAAGATTATAACTTAGAATCTTTATCGATTGAATTCTGTGCCATGGAAGTAGAAGCTAAAGAATTAGGGTATAGCTTCGGACTTTCTATCAAGAGACCTGATAGTTTAAAGTATTCTGAGGTTTGAATAAGCTATTAATGTTTCATACCGCTGTTTGCATATCTAAGTCTTTCTGAGTCCAGGTTGAGCTTGTTCACTCTCAATTCAAAAGAGGTAAAGGAAGGATTGATACACAAGATGCCCTGCTGTATGTTAACCTCCACTCTCTCTATAACAACAGCAAAGGGATTGGATGAGAAGTTGGAGGTAATGGATATAGCTGATCGTACTCAAGAGTCTCTTGCTTTACATGAAATGGTTTCAGCTAGCAATGGAGATCCATGGGAGAAATTTGAGAAAATGTCACTTCTATTGAAGAAATCGTGTGGCCTTTGCTGAAGCTGGTGCAATCCCTCCTCTTGTCGGCCTCCTTTCAACAACGGACCTCCTCACTCAAGAACAGTCTGTGACAATAATAAGAGAAGTATCATGTCCTGTAGGGTAGCACCTGGCATAGTCCATGAGCTGAAGTCGGGGAGTATGGAAGCACGTGAAAATGCTGCAGCCACTCTTTTCAGCCTTTCTGTTGTCGACGAATATAAAACAACTGCTCTTTTCAACTTGTGTTTCTACCGAGGGGACAAGGTAAAGGCAGTCAGGAGTCCCAGATTGTTTCCACACTGATGCAGCTGTTGATAGAATCTATAATAGAGATGATGGCTCTGAAGGACGTCCCCGTTTTAGTCGATTTCATTGCAACAGGATTCCCTCGGAACAGGGAGAATGCCGCTGCAGTTTTGGTGAACCTTTGTGCAGGAGACAAGCGACATCTCGTGGAAGCAAAGAAGCCCGGAGTGACTAGTTTGCTAATAGATTTGGCATAA

mRNA sequence

ATGTGGGATATGAATCAGGCGAATGATACTGTGAGAGCTCTCGCGTCGTTGAAGGAAGCTCTGGATTCGACCAAGGAGTTGCTGAGACTTGGGAGCGAGGGGAGCAAGAATGCTATAAACAATGTTTCGATGGACATCTTCCGGTTCAACCACACTTTGCTGATGCCTTCCATTCGATTAGACGACGTTCGCATTGCAGCTGTTATCTCGTACTTGCACCTTGTATTAATTTACGTTCTGTCTAAATTTCTGCCTTTATTCTCAATTGATCATCATGATTTTGCTGAAGATTGGGAAAATGCCTTGGAGAAAGAGCAAAATTTGAATCAATTTCACAGGATGACAGCAAGACTAGAGAAAGCTCTGAATGAAATTTCACATGAAGATCTTGATATATCTGATGAAGTTAAGGAGAAGGTTGAGCTTGTTCACTCTCAATTCAAAAGAGCTAGCAATGGAGATCCATGGGAGAAATTTGAGAAAATGTCACTTCTATTGAAGAAATCGGTAGCACCTGGCATAGTCCATGAGCTGAAGTCGGGGAGTATGGAAGCACGTGAAAATGCTGCAGCCACTCTTTTCAGCCTTTCTGTTGTCGACGAATATAAAACAACTGCTCTTTTCAACTTGTGTTTCTACCGAGGGGACAAGGACGTCCCCGTTTTAGTCGATTTCATTGCAACAGGATTCCCTCGGAACAGGGAGAATGCCGCTGCAGTTTTGGTGAACCTTTGTGCAGGAGACAAGCGACATCTCGTGGAAGCAAAGAAGCCCGGAGTGACTAGTTTGCTAATAGATTTGGCATAA

Coding sequence (CDS)

ATGTGGGATATGAATCAGGCGAATGATACTGTGAGAGCTCTCGCGTCGTTGAAGGAAGCTCTGGATTCGACCAAGGAGTTGCTGAGACTTGGGAGCGAGGGGAGCAAGAATGCTATAAACAATGTTTCGATGGACATCTTCCGGTTCAACCACACTTTGCTGATGCCTTCCATTCGATTAGACGACGTTCGCATTGCAGCTGTTATCTCGTACTTGCACCTTGTATTAATTTACGTTCTGTCTAAATTTCTGCCTTTATTCTCAATTGATCATCATGATTTTGCTGAAGATTGGGAAAATGCCTTGGAGAAAGAGCAAAATTTGAATCAATTTCACAGGATGACAGCAAGACTAGAGAAAGCTCTGAATGAAATTTCACATGAAGATCTTGATATATCTGATGAAGTTAAGGAGAAGGTTGAGCTTGTTCACTCTCAATTCAAAAGAGCTAGCAATGGAGATCCATGGGAGAAATTTGAGAAAATGTCACTTCTATTGAAGAAATCGGTAGCACCTGGCATAGTCCATGAGCTGAAGTCGGGGAGTATGGAAGCACGTGAAAATGCTGCAGCCACTCTTTTCAGCCTTTCTGTTGTCGACGAATATAAAACAACTGCTCTTTTCAACTTGTGTTTCTACCGAGGGGACAAGGACGTCCCCGTTTTAGTCGATTTCATTGCAACAGGATTCCCTCGGAACAGGGAGAATGCCGCTGCAGTTTTGGTGAACCTTTGTGCAGGAGACAAGCGACATCTCGTGGAAGCAAAGAAGCCCGGAGTGACTAGTTTGCTAATAGATTTGGCATAA

Protein sequence

MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRLDDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDKDVPVLVDFIATGFPRNRENAAAVLVNLCAGDKRHLVEAKKPGVTSLLIDLA
Homology
BLAST of Csor.00g011440 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-22
Identity = 73/179 (40.78%), Postives = 84/179 (46.93%), Query Frame = 0

Query: 165 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYK--------------------- 224
           ++     PGIV  LK GSMEARENAAATLFSLSV+DE K                     
Sbjct: 431 IVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLNEGTQ 490

Query: 225 ------TTALFNLCFYRGDKD--------------------------------------- 269
                  TALFNLC Y+G+K                                        
Sbjct: 491 RGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEG 550


HSP 2 Score: 51.6 bits (122), Expect = 1.6e-05
Identity = 30/71 (42.25%), Postives = 49/71 (69.01%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRAS---NGDPWEK 161
           +E+EQ  ++   ++ +LE++L++I +E+LDISDEV+E+VELV SQF+RA    +    E 
Sbjct: 92  MEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQVELVLSQFRRAKGRVDVSDDEL 151

Query: 162 FEKMSLLLKKS 170
           +E +  L  KS
Sbjct: 152 YEDLQSLCNKS 162

BLAST of Csor.00g011440 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.1e-21
Identity = 68/183 (37.16%), Postives = 88/183 (48.09%), Query Frame = 0

Query: 161 KMSLLLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYK----------------- 220
           K  ++      PGIVH L+ GSMEARENAAATLFSLSV+DE K                 
Sbjct: 432 KGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKVTIGAAGAIPPLVTLLS 491

Query: 221 ----------TTALFNLCFYRGDKD----------------------------------- 269
                      TALFNLC ++G+K                                    
Sbjct: 492 EGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESLSILAILSS 551


HSP 2 Score: 46.6 bits (109), Expect = 5.1e-04
Identity = 24/49 (48.98%), Postives = 40/49 (81.63%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRA 151
           LE++Q + +F ++T+ LE+AL+ I +E+L+ISDE+KE+VELV  Q +R+
Sbjct: 92  LERDQVMVKFQKVTSLLEQALSIIPYENLEISDELKEQVELVLVQLRRS 140

BLAST of Csor.00g011440 vs. ExPASy Swiss-Prot
Match: A2ZLU6 (Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 3.7e-18
Identity = 65/181 (35.91%), Postives = 82/181 (45.30%), Query Frame = 0

Query: 165 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTT------------------- 224
           ++     P IVH LK+GSMEARENAAATLFSLSV+DEYK T                   
Sbjct: 446 IISSGAVPSIVHVLKNGSMEARENAAATLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQ 505

Query: 225 --------ALFNLCFYRGDK---------------------------------------- 269
                   ALFNLC Y+G+K                                        
Sbjct: 506 RGKKDAAAALFNLCIYQGNKGRAIRAGLVPLIMGLVTNPTGALMDEAMAILSILSSHPEG 565


HSP 2 Score: 50.4 bits (119), Expect = 3.6e-05
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRA 151
           LE++  + +F  +  +LE+AL +I + +LDISDEV+E+VELVH+Q KRA
Sbjct: 114 LERDSVMKKFQGVILQLEQALCDIPYNELDISDEVREQVELVHAQLKRA 162

BLAST of Csor.00g011440 vs. ExPASy Swiss-Prot
Match: Q0IMG9 (E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 3.7e-18
Identity = 65/181 (35.91%), Postives = 82/181 (45.30%), Query Frame = 0

Query: 165 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTT------------------- 224
           ++     P IVH LK+GSMEARENAAATLFSLSV+DEYK T                   
Sbjct: 446 IISSGAVPSIVHVLKNGSMEARENAAATLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQ 505

Query: 225 --------ALFNLCFYRGDK---------------------------------------- 269
                   ALFNLC Y+G+K                                        
Sbjct: 506 RGKKDAAAALFNLCIYQGNKGRAIRAGLVPLIMGLVTNPTGALMDEAMAILSILSSHPEG 565


HSP 2 Score: 50.4 bits (119), Expect = 3.6e-05
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRA 151
           LE++  + +F  +  +LE+AL +I + +LDISDEV+E+VELVH+Q KRA
Sbjct: 114 LERDSVMKKFQGVILQLEQALCDIPYNELDISDEVREQVELVHAQLKRA 162

BLAST of Csor.00g011440 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 5.5e-14
Identity = 60/172 (34.88%), Postives = 71/172 (41.28%), Query Frame = 0

Query: 172 PGIVHELKSGSMEARENAAATLFSLSVVDEYK---------------------------T 231
           P IV  LK+GSME RENAAATLFSLSVVDE K                            
Sbjct: 409 PKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSPRGKKDAA 468

Query: 232 TALFNLCFYRGDK----------------------------------------------- 269
           TA+FNLC Y+G+K                                               
Sbjct: 469 TAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPEGKIVIARS 528

BLAST of Csor.00g011440 vs. NCBI nr
Match: KAG6605467.1 (U-box domain-containing protein 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520 bits (1339), Expect = 9.28e-186
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL 60
           MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL
Sbjct: 1   MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL 60

Query: 61  DDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK 120
           DDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK
Sbjct: 61  DDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK 120

Query: 121 ALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKSVAPGIVHELKS 180
           ALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKSVAPGIVHELKS
Sbjct: 121 ALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKSVAPGIVHELKS 180

Query: 181 GSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDKDVPVLVDFIATGFPRNRENAAAV 240
           GSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDKDVPVLVDFIATGFPRNRENAAAV
Sbjct: 181 GSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDKDVPVLVDFIATGFPRNRENAAAV 240

Query: 241 LVNLCAGDKRHLVEAKKPGVTSLLIDLA 268
           LVNLCAGDKRHLVEAKKPGVTSLLIDLA
Sbjct: 241 LVNLCAGDKRHLVEAKKPGVTSLLIDLA 268

BLAST of Csor.00g011440 vs. NCBI nr
Match: KAG7035412.1 (U-box domain-containing protein 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 465 bits (1197), Expect = 1.01e-163
Identity = 254/297 (85.52%), Postives = 255/297 (85.86%), Query Frame = 0

Query: 1   MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL 60
           MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL
Sbjct: 1   MWDMNQANDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRL 60

Query: 61  DDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK 120
           DDVRIAAVISYLHL    VLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK
Sbjct: 61  DDVRIAAVISYLHL----VLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEK 120

Query: 121 ALNEISHEDLDISDEVKEK----------------VELVHSQFKRASNGDPWEKFEKMSL 180
           ALNEISHEDLDISDEVKEK                  L   +   ASNGDPWEKFEKMSL
Sbjct: 121 ALNEISHEDLDISDEVKEKGLDEKLEVMDIADRTQESLALHEMVSASNGDPWEKFEKMSL 180

Query: 181 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDK------- 240
           LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTTALFNLCFYR DK       
Sbjct: 181 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTTALFNLCFYRWDKLLIESII 240

Query: 241 ------DVPVLVDFIATGFPRNRENAAAVLVNLCAGDKRHLVEAKKPGVTSLLIDLA 268
                 DVPVLVDFIATGFPRNRENAAAVLVNLCAGDKRHLVEAKKPGVTSLLIDLA
Sbjct: 241 EMMALKDVPVLVDFIATGFPRNRENAAAVLVNLCAGDKRHLVEAKKPGVTSLLIDLA 293

BLAST of Csor.00g011440 vs. NCBI nr
Match: XP_023532328.1 (U-box domain-containing protein 13-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 123 bits (308), Expect = 1.89e-31
Identity = 83/151 (54.97%), Postives = 85/151 (56.29%), Query Frame = 0

Query: 1   MWDMNQA--NDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSI 60
           MWDMNQA  NDTVRALASLKEALDS KELLRLGSEGS N +                   
Sbjct: 1   MWDMNQALPNDTVRALASLKEALDSPKELLRLGSEGS-NCL------------------- 60

Query: 61  RLDDVRIAAVISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARL 120
                                                     ALEKEQNLNQFHRMTARL
Sbjct: 61  ------------------------------------------ALEKEQNLNQFHRMTARL 89

Query: 121 EKALNEISHEDLDISDEVKEKVELVHSQFKR 149
           EKALNEISHEDLDISDEVKE+VELVHSQFKR
Sbjct: 121 EKALNEISHEDLDISDEVKEQVELVHSQFKR 89

BLAST of Csor.00g011440 vs. NCBI nr
Match: PPR84579.1 (hypothetical protein GOBAR_AA36131 [Gossypium barbadense])

HSP 1 Score: 125 bits (314), Expect = 8.72e-29
Identity = 138/471 (29.30%), Postives = 164/471 (34.82%), Query Frame = 0

Query: 9   DTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRLDDVRIAAV 68
           +TV+AL SL+EAL STKELL  GSEGSK                                
Sbjct: 60  ETVKALLSLEEALISTKELLSFGSEGSK-------------------------------- 119

Query: 69  ISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEKALNEISHE 128
                   IY++                     LE+EQ +++F  +TA+LE+AL  IS+E
Sbjct: 120 --------IYLV---------------------LEREQIMHKFLEVTAQLEQALRGISYE 179

Query: 129 DLDISDEVKEKVELVHSQFKRA-------------------------------------- 188
           +LDISDEVKE+VELV +QF+RA                                      
Sbjct: 180 NLDISDEVKEQVELVLAQFRRAKGRVDAPDLELYEDLLSLYNKSDDAELDPDVLRRLAKK 239

Query: 189 ----------------------SNGDPWEKFEKMSLLLKK-------------------- 248
                                 S GDP E FEKMS LLKK                    
Sbjct: 240 LQLVGIAELTQESLALHEMVSASGGDPGETFEKMSSLLKKIKDFIQTENPNLDAPAREKN 299

Query: 249 --------------------------------------------------------SVAP 268
                                                                      P
Sbjct: 300 LPPSSSGQATTDGNRKTPVIPDDFRCPISLELMKDPVIVSTGQLLNVNNKGSIISSGAVP 359

BLAST of Csor.00g011440 vs. NCBI nr
Match: XP_038901464.1 (U-box domain-containing protein 13-like [Benincasa hispida] >XP_038901465.1 U-box domain-containing protein 13-like [Benincasa hispida])

HSP 1 Score: 120 bits (300), Expect = 1.34e-26
Identity = 159/609 (26.11%), Postives = 180/609 (29.56%), Query Frame = 0

Query: 8   NDTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRLDDVRIAA 67
           +DTVRALASLKEAL+STKELLR GSEGSK                               
Sbjct: 60  DDTVRALASLKEALESTKELLRQGSEGSK------------------------------- 119

Query: 68  VISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEKALNEISH 127
                    IY+                     AL++EQ LNQFH +TARLE+ALNEISH
Sbjct: 120 ---------IYL---------------------ALDREQILNQFHEVTARLEQALNEISH 179

Query: 128 EDLDISDEVKEKVELVHSQFKRA------------------------------------- 187
           EDLDISDEVKE+VELV SQF+RA                                     
Sbjct: 180 EDLDISDEVKEQVELVLSQFRRARGRNDTQDAEVYVNLNSLYNNNRDSADPSILKVLAEK 239

Query: 188 ----------------------SNGDPWEKFEKMSLLLKK-------------------- 247
                                 S+GDP  +FEKMS+LLKK                    
Sbjct: 240 LQLIVIADLTQESLALHEMVLASDGDPGGRFEKMSILLKKIKDFMLTENPEIGLSSKEQS 299

Query: 248 ------------------------------------------------------------ 268
                                                                       
Sbjct: 300 HPRTGGQVSAGKKDIVSLHIPEDFRCPISLDLMKDPVIVSTGQTYERAFIEKWLADGHST 359

BLAST of Csor.00g011440 vs. ExPASy TrEMBL
Match: A0A2P5W0G5 (RING-type E3 ubiquitin transferase OS=Gossypium barbadense OX=3634 GN=GOBAR_AA36131 PE=4 SV=1)

HSP 1 Score: 125 bits (314), Expect = 4.22e-29
Identity = 138/471 (29.30%), Postives = 164/471 (34.82%), Query Frame = 0

Query: 9   DTVRALASLKEALDSTKELLRLGSEGSKNAINNVSMDIFRFNHTLLMPSIRLDDVRIAAV 68
           +TV+AL SL+EAL STKELL  GSEGSK                                
Sbjct: 60  ETVKALLSLEEALISTKELLSFGSEGSK-------------------------------- 119

Query: 69  ISYLHLVLIYVLSKFLPLFSIDHHDFAEDWENALEKEQNLNQFHRMTARLEKALNEISHE 128
                   IY++                     LE+EQ +++F  +TA+LE+AL  IS+E
Sbjct: 120 --------IYLV---------------------LEREQIMHKFLEVTAQLEQALRGISYE 179

Query: 129 DLDISDEVKEKVELVHSQFKRA-------------------------------------- 188
           +LDISDEVKE+VELV +QF+RA                                      
Sbjct: 180 NLDISDEVKEQVELVLAQFRRAKGRVDAPDLELYEDLLSLYNKSDDAELDPDVLRRLAKK 239

Query: 189 ----------------------SNGDPWEKFEKMSLLLKK-------------------- 248
                                 S GDP E FEKMS LLKK                    
Sbjct: 240 LQLVGIAELTQESLALHEMVSASGGDPGETFEKMSSLLKKIKDFIQTENPNLDAPAREKN 299

Query: 249 --------------------------------------------------------SVAP 268
                                                                      P
Sbjct: 300 LPPSSSGQATTDGNRKTPVIPDDFRCPISLELMKDPVIVSTGQLLNVNNKGSIISSGAVP 359

BLAST of Csor.00g011440 vs. ExPASy TrEMBL
Match: A0A0A0KFG3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G191020 PE=4 SV=1)

HSP 1 Score: 119 bits (299), Expect = 8.79e-27
Identity = 78/173 (45.09%), Postives = 87/173 (50.29%), Query Frame = 0

Query: 171 APGIVHELKSGSMEARENAAATLFSLSVVDEYKT-------------------------- 230
           APGIVH LK GSMEARENAAATLFSLSVVDEYK                           
Sbjct: 434 APGIVHVLKWGSMEARENAAATLFSLSVVDEYKVMIGASGAILPLIALLNEGTQRGKKDA 493

Query: 231 -TALFNLCFYRGDK---------------------------------------------- 268
            TALFNLCF++G+K                                              
Sbjct: 494 ATALFNLCFFQGNKIKAVRGGVVSILMQLLTESRIGMVDEALAILAILANNSEGRAAIGA 553

BLAST of Csor.00g011440 vs. ExPASy TrEMBL
Match: A0A5A7V197 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00630 PE=4 SV=1)

HSP 1 Score: 116 bits (291), Expect = 1.04e-25
Identity = 77/173 (44.51%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 171 APGIVHELKSGSMEARENAAATLFSLSVVDEYK--------------------------- 230
           AP IVH LK GSMEARENAAATLFSLSVVDEYK                           
Sbjct: 435 APAIVHVLKWGSMEARENAAATLFSLSVVDEYKIMIGASGAILPLIALLNEGTQRGKKDA 494

Query: 231 TTALFNLCFYRGDK---------------------------------------------- 268
            TALFNLCF++G+K                                              
Sbjct: 495 ATALFNLCFFQGNKIKAVRGGVVSILMQLLTESRIGMVDEALAILAILASNSEGRAAIGA 554

BLAST of Csor.00g011440 vs. ExPASy TrEMBL
Match: A0A1S3BJL0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490599 PE=4 SV=1)

HSP 1 Score: 116 bits (291), Expect = 1.04e-25
Identity = 77/173 (44.51%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 171 APGIVHELKSGSMEARENAAATLFSLSVVDEYK--------------------------- 230
           AP IVH LK GSMEARENAAATLFSLSVVDEYK                           
Sbjct: 435 APAIVHVLKWGSMEARENAAATLFSLSVVDEYKIMIGASGAILPLIALLNEGTQRGKKDA 494

Query: 231 TTALFNLCFYRGDK---------------------------------------------- 268
            TALFNLCF++G+K                                              
Sbjct: 495 ATALFNLCFFQGNKIKAVRGGVVSILMQLLTESRIGMVDEALAILAILASNSEGRAAIGA 554

BLAST of Csor.00g011440 vs. ExPASy TrEMBL
Match: A0A6J1FVM5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448510 PE=4 SV=1)

HSP 1 Score: 114 bits (286), Expect = 4.84e-25
Identity = 77/173 (44.51%), Postives = 85/173 (49.13%), Query Frame = 0

Query: 171 APGIVHELKSGSMEARENAAATLFSLSVVDEYKT-------------------------- 230
           APGIVH LK GSMEARENAAATLFSLSVVDEYK                           
Sbjct: 437 APGIVHVLKWGSMEARENAAATLFSLSVVDEYKVMIGASGAIPPLIALLNEGTQRGKKDA 496

Query: 231 -TALFNLCFYRGDK---------------------------------------------- 268
            TALFNLCFY+G+K                                              
Sbjct: 497 ATALFNLCFYQGNKVKAVRGGVVSILMQLLTESRIGMVDEALAILAILASNSEGRAAIGA 556

BLAST of Csor.00g011440 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 107.1 bits (266), Expect = 2.3e-23
Identity = 73/179 (40.78%), Postives = 84/179 (46.93%), Query Frame = 0

Query: 165 LLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYK--------------------- 224
           ++     PGIV  LK GSMEARENAAATLFSLSV+DE K                     
Sbjct: 431 IVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLNEGTQ 490

Query: 225 ------TTALFNLCFYRGDKD--------------------------------------- 269
                  TALFNLC Y+G+K                                        
Sbjct: 491 RGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEG 550


HSP 2 Score: 51.6 bits (122), Expect = 1.1e-06
Identity = 30/71 (42.25%), Postives = 49/71 (69.01%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRAS---NGDPWEK 161
           +E+EQ  ++   ++ +LE++L++I +E+LDISDEV+E+VELV SQF+RA    +    E 
Sbjct: 92  MEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQVELVLSQFRRAKGRVDVSDDEL 151

Query: 162 FEKMSLLLKKS 170
           +E +  L  KS
Sbjct: 152 YEDLQSLCNKS 162

BLAST of Csor.00g011440 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 104.4 bits (259), Expect = 1.5e-22
Identity = 68/183 (37.16%), Postives = 88/183 (48.09%), Query Frame = 0

Query: 161 KMSLLLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYK----------------- 220
           K  ++      PGIVH L+ GSMEARENAAATLFSLSV+DE K                 
Sbjct: 432 KGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKVTIGAAGAIPPLVTLLS 491

Query: 221 ----------TTALFNLCFYRGDKD----------------------------------- 269
                      TALFNLC ++G+K                                    
Sbjct: 492 EGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESLSILAILSS 551


HSP 2 Score: 46.6 bits (109), Expect = 3.7e-05
Identity = 24/49 (48.98%), Postives = 40/49 (81.63%), Query Frame = 0

Query: 102 LEKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRA 151
           LE++Q + +F ++T+ LE+AL+ I +E+L+ISDE+KE+VELV  Q +R+
Sbjct: 92  LERDQVMVKFQKVTSLLEQALSIIPYENLEISDELKEQVELVLVQLRRS 140

BLAST of Csor.00g011440 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 78.6 bits (192), Expect = 8.7e-15
Identity = 66/205 (32.20%), Postives = 83/205 (40.49%), Query Frame = 0

Query: 151 SNGDPWEKFEKMSLLLKKSVAPG-------------IVHELKSGSMEARENAAATLFSLS 210
           S+ DP  +   ++ LL  S+  G             IV  LK+GSMEARENAAATLFSLS
Sbjct: 397 SSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAATLFSLS 456

Query: 211 VVDEYK---------------------------TTALFNLCFYRGDK------------- 268
           V+DE K                            TA+FNLC Y+G+K             
Sbjct: 457 VIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLT 516


HSP 2 Score: 48.5 bits (114), Expect = 9.6e-06
Identity = 25/69 (36.23%), Postives = 46/69 (66.67%), Query Frame = 0

Query: 103 EKEQNLNQFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKM 162
           +++  + +F  MT  +E AL++I +E +++S+EV+E+V+L+H QFKRA      E++E+ 
Sbjct: 97  DRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLHFQFKRAK-----ERWEES 156

Query: 163 SLLLKKSVA 172
            L L   +A
Sbjct: 157 DLQLSHDLA 160

BLAST of Csor.00g011440 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-12
Identity = 43/106 (40.57%), Postives = 59/106 (55.66%), Query Frame = 0

Query: 164 LLLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYKTTALFNLCFYRGDKDVPVLV 223
           L++       IV  L++G+MEARENAAATLFSLS+ DE       N     G   +P LV
Sbjct: 409 LIMFAGAVTSIVQVLRAGTMEARENAAATLFSLSLADE-------NKIIIGGSGAIPALV 468

Query: 224 DFIATGFPRNRENAAAVLVNLCA--GDKRHLVEAKKPGVTSLLIDL 268
           D +  G PR +++AA  L NLC   G+K   V A   G+ + L+ +
Sbjct: 469 DLLENGTPRGKKDAATALFNLCIYHGNKGRAVRA---GIVTALVKM 504


HSP 2 Score: 42.0 bits (97), Expect = 9.0e-04
Identity = 31/85 (36.47%), Postives = 46/85 (54.12%), Query Frame = 0

Query: 110 QFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKS 169
           QF  +T +LEKAL+ + ++  DISDEV E+VEL  SQ +RA          K S  L + 
Sbjct: 103 QFQCVTWKLEKALSNLPYDLYDISDEVGEQVELARSQLRRAMQRYGSLNSNKFSSALSEP 162

Query: 170 V-APGIVHELKSGSMEARENAAATL 194
           +   G  + +K  + E  E+ + TL
Sbjct: 163 MERDGFSNVIKIKAEEKLESVSETL 187

BLAST of Csor.00g011440 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-11
Identity = 54/181 (29.83%), Postives = 71/181 (39.23%), Query Frame = 0

Query: 164 LLLKKSVAPGIVHELKSGSMEARENAAATLFSLSVVDEYK-------------------- 223
           L++       IV  L++GSMEARENAAATLFSLS+ DE K                    
Sbjct: 420 LIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGS 479

Query: 224 -------TTALFNLCFYRGDKD-------------------------------------- 269
                   TALFNLC Y+G+K                                       
Sbjct: 480 VRGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQ 539


HSP 2 Score: 42.7 bits (99), Expect = 5.3e-04
Identity = 33/100 (33.00%), Postives = 53/100 (53.00%), Query Frame = 0

Query: 110 QFHRMTARLEKALNEISHEDLDISDEVKEKVELVHSQFKRASNGDPWEKFEKMSLLLKKS 169
           QF  +T +LEKAL +++++  DISDEV+E+VEL   Q +RA         ++   L  K 
Sbjct: 108 QFQCVTWKLEKALGDLTYDRYDISDEVREQVELARLQLRRA--------MQRYGSLNSKK 167

Query: 170 VAPGIVHELKSGS------MEARENAAATLFSLSVVDEYK 204
            + G+   ++  +      +E  E+   T+ SLS  DE K
Sbjct: 168 FSSGLSEPMEKDASSNRKVIEKLESIPETVHSLS--DEKK 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SNC63.2e-2240.78U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Q9ZV312.1e-2137.16U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
A2ZLU63.7e-1835.91Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=... [more]
Q0IMG93.7e-1835.91E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SP... [more]
Q5VRH95.5e-1434.88U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Match NameE-valueIdentityDescription
KAG6605467.19.28e-186100.00U-box domain-containing protein 13, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7035412.11.01e-16385.52U-box domain-containing protein 13, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023532328.11.89e-3154.97U-box domain-containing protein 13-like isoform X1 [Cucurbita pepo subsp. pepo][more]
PPR84579.18.72e-2929.30hypothetical protein GOBAR_AA36131 [Gossypium barbadense][more]
XP_038901464.11.34e-2626.11U-box domain-containing protein 13-like [Benincasa hispida] >XP_038901465.1 U-bo... [more]
Match NameE-valueIdentityDescription
A0A2P5W0G54.22e-2929.30RING-type E3 ubiquitin transferase OS=Gossypium barbadense OX=3634 GN=GOBAR_AA36... [more]
A0A0A0KFG38.79e-2745.09RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G191020 PE... [more]
A0A5A7V1971.04e-2544.51RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BJL01.04e-2544.51RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490599 PE=4 ... [more]
A0A6J1FVM54.84e-2544.51RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448510... [more]
Match NameE-valueIdentityDescription
AT3G46510.12.3e-2340.78plant U-box 13 [more]
AT2G28830.11.5e-2237.16PLANT U-BOX 12 [more]
AT3G54850.18.7e-1532.20plant U-box 14 [more]
AT1G23030.13.1e-1240.57ARM repeat superfamily protein [more]
AT1G71020.11.2e-1129.83ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 141..268
e-value: 6.8E-9
score: 37.5
NoneNo IPR availablePANTHERPTHR23315:SF275U-BOX DOMAIN-CONTAINING PROTEIN 12coord: 171..205
coord: 218..268
coord: 8..160
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 171..205
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 218..268
coord: 8..160
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 165..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g011440.m01Csor.00g011440.m01mRNA