Csor.00g005350 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g005350
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	gibberellin 2-beta-dioxygenase 2-like
Location	Csor_Chr08: 2843622 .. 2844898 (+)
RNA-Seq Expression	Csor.00g005350
Synteny	Csor.00g005350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTTTCCGACAGCCATGAGATCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCTTGTGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACACCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCCGAGAAGCGCCGTGCTGGCCCTGCCACCCCCTTCGGCTATGGCTGCAAAGCCATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCTGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGGTACTGCTTAGCTAGAAGAAAAAAAAATTGAGTTTTTAACTGAAACATTGATTTATGTCGACATGTCGTCTGATATCTTAATTTGATGCAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGTAAATTCTATGTCATTCATCTGTAGATCAAAGTATAAAAACTATTATATAAGATGCACGTAACAAGTCTAGTGTCAAACGAGACGTCGATTTTTTACTTAAAACCTAAAGAACGTAACATGATCACGAACATTAGAAGCAAAAACATTTACAAAATGGGTTATTGAGACTTGTTCTTGTTTTTGATTAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

mRNA sequence

ATGCCTTTTCCGACAGCCATGAGATCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCTTGTGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACACCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCCGAGAAGCGCCGTGCTGGCCCTGCCACCCCCTTCGGCTATGGCTGCAAAGCCATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCTGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

Coding sequence (CDS)

ATGCCTTTTCCGACAGCCATGAGATCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCTTGTGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACACCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCCGAGAAGCGCCGTGCTGGCCCTGCCACCCCCTTCGGCTATGGCTGCAAAGCCATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCTGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

Protein sequence

MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNSPLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS

Homology

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BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.2e-108
Identity = 193/331 (58.31%), Postives = 250/331 (75.53%), Query Frame = 0


Query: 1   MPFPTAM-RSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 60
           +P PT+M R+KKTKAVGIPTIDLS  R++ S+ +V+ACE +G FKV+NH V    I+ L+
Sbjct: 3   VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62

Query: 61  HQAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS- 120
            +  +FF + + EKR+AG +TPFGYGCK IGPNGD G+LEY+LL S+P SI+E S +I+ 
Sbjct: 63  EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122

Query: 121 NSPLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 180
           + P+ FS +VN+Y+K+V++L CEIL+L  EGLW+P+KS  S +I+D  SDS+LRIN+YP 
Sbjct: 123 DHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHYPP 182

Query: 181 AVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDP 240
             K    + + S   + + N IGFGEHSDPQILTILRSN+V GLQIS H GLW PV PDP
Sbjct: 183 VKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDP 242

Query: 241 SAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTK 300
           S FYV++GDA Q +TN RFVSVRHR + N + K RMS+MYF APPLN  I+P S M+   
Sbjct: 243 SEFYVMVGDALQVLTNGRFVSVRHRVLTNTT-KPRMSMMYFAAPPLNWLISPLSKMVTAH 302

Query: 301 DQSLYKPFTWAQYKKAAYSLRLADSRLNLFK 330
              LY+PFTWAQYK+AAY+LRL D+RL+ FK
Sbjct: 303 SPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332

[Close]

BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.0e-90
Identity = 169/327 (51.68%), Postives = 216/327 (66.06%), Query Frame = 0


Query: 5   TAMRSKKTKAVGIPTIDLS-ESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAF 64
           T  ++  +     P ID S   R++ S+ IV+ACE  G FKVINHGV    I   EH+  
Sbjct: 12  TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71

Query: 65  DFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS-NSPL 124
           +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LL ++P ++A+ S +IS + P 
Sbjct: 72  EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131

Query: 125 GFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKH 184
            FS   N+Y+++VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP A   
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA--- 191

Query: 185 TEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFY 244
                     +     +IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP+ F+
Sbjct: 192 --------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFF 251

Query: 245 VLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSL 304
           VL+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+  ++   
Sbjct: 252 VLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRR 311

Query: 305 YKPFTWAQYKKAAYSLRLADSRLNLFK 330
           Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 312 YNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-78
Identity = 165/326 (50.61%), Postives = 218/326 (66.87%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+ACE  G FKVINHGV    I+ +E ++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPAT-PFGYGCKAIGPNGDIGDLEYILLR-SDPASIAETSASISNSPLGF 128
            + A EK+   P   PF YG + IG NGD G++EY+L   +DPA           S L F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y+++V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVL 248
           ++  LS +S +S  ++GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF V 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++T +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLNLFK 330
           + FTW +YKK AYSLRL DSRL++F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.5e-77
Identity = 146/314 (46.50%), Postives = 209/314 (66.56%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+ACE FG FKV+NHGV    +  LE +A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPATPFGYGCKAIGPNGDIGDLEYILLRSDP-ASIAETSASISNSPLGFSYVVNEYVKSV 136
           GP  P+GYG K IGPNGD+G +EY+LL ++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK          
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEK---------- 210

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
              K+GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F++ +GDA Q MTN 
Sbjct: 211 -MVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLNLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-76
Identity = 147/317 (46.37%), Postives = 206/317 (64.98%), Query Frame = 0


Query: 16  GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTAPEKRR 75
           GIP +DL+   A+    IV AC  FG FK++NHGV    +ANLE++A  FF ++  EK R
Sbjct: 26  GIPVVDLTHPDAK--NLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85

Query: 76  AGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASI-SNSPLGFSYVVNEYVKS 135
           AGP  PFGYG K IGPNGD+G +EY+LL ++P  I+  S  I   +P  F  VV  Y+ +
Sbjct: 86  AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITA 145

Query: 136 VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSH 195
           V+ +   +L+L+ EGL +  ++  S L++D +SDS  R+N+YP                 
Sbjct: 146 VKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPC----------PEVQA 205

Query: 196 LSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTN 255
           L+ N +GFGEH+DPQI+++LRSN   GLQI L DG W  V PD ++F++ +GDA Q MTN
Sbjct: 206 LNRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTN 265

Query: 256 DRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKA 315
            RF SV+HR +A+ + K+R+S++YFG P L+  IAP   +ML  ++ LYK FTW +YKKA
Sbjct: 266 GRFKSVKHRVLADTT-KSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKA 325

Query: 316 AYSLRLADSRLNLFKAS 332
           AY+ RLAD+RL  F+ S
Sbjct: 326 AYTSRLADNRLAPFQKS 329

[Close]

BLAST of Csor.00g005350 vs. NCBI nr
Match: KAG6593256.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669 bits (1726), Expect = 1.25e-242
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. NCBI nr
Match: KAG7025608.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 664 bits (1712), Expect = 1.70e-240
Identity = 329/331 (99.40%), Postives = 329/331 (99.40%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. NCBI nr
Match: XP_022959634.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])

HSP 1 Score: 662 bits (1708), Expect = 6.91e-240
Identity = 328/331 (99.09%), Postives = 328/331 (99.09%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. NCBI nr
Match: XP_023514766.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658 bits (1698), Expect = 2.31e-238
Identity = 325/331 (98.19%), Postives = 327/331 (98.79%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFGIFKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEK RAGPA PFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKHRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNK+GFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKVGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. NCBI nr
Match: XP_023004594.1 (gibberellin 2-beta-dioxygenase 2 [Cucurbita maxima])

HSP 1 Score: 648 bits (1672), Expect = 2.13e-234
Identity = 320/331 (96.68%), Postives = 323/331 (97.58%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTA RSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFG FKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILL SDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PL FSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTN IGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FY+LIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL
Match: A0A6J1H8N0 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460653 PE=3 SV=1)

HSP 1 Score: 662 bits (1708), Expect = 3.35e-240
Identity = 328/331 (99.09%), Postives = 328/331 (99.09%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL
Match: A0A6J1KWQ4 (gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=3 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 1.03e-234
Identity = 320/331 (96.68%), Postives = 323/331 (97.58%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MPFPTA RSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFG FKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILL SDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PL FSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTN IGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FY+LIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331

[Close]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL
Match: A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 2.93e-197
Identity = 277/331 (83.69%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MP PTAMR+KKTKAVGIPTIDLS++R  TSKSI+ AC HFG FK+INHGVS HAI+ LE 
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
            AFDFF +TAPEK RAGPA PFGYG KAIGPNGD GDLEY+LLRSDPAS+ +TS SISN 
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSYVVNEYVKSV++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPA V
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPA-V 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTE + ELSSKS+ S N+IGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTE-NWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDA QAMTN RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAP S+M+L   Q
Sbjct: 241 FYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

[Close]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL
Match: A0A6J1JXB5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488342 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.97e-195
Identity = 275/331 (83.08%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MP PTAMR+KKT+AVGIPTIDLS++R  TSKSI+ AC HFG FK+INHGVS HAI+ LE 
Sbjct: 1   MPCPTAMRTKKTEAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLEC 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFF +TAPEK RAGPA PFGYG KAIGPNGD GDLEY+LLRSDPAS+ +TS SISN 
Sbjct: 61  QAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSYVVNEYVKSV++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYPA V
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPA-V 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTE + ELSSKS+ S N+IGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTE-NWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDA QAMTN RFVSVRHRAMAN+SKKARMSIMYFGAPPLNAWIAP S+M+    Q
Sbjct: 241 FYVLIGDALQAMTNGRFVSVRHRAMANSSKKARMSIMYFGAPPLNAWIAPLSEMIPPNHQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

[Close]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL
Match: A0A6J1DEJ9 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 PE=3 SV=1)

HSP 1 Score: 540 bits (1390), Expect = 9.58e-192
Identity = 271/332 (81.63%), Postives = 292/332 (87.95%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
           MP PTAMR+KK KAVGIPTIDLSESRARTSK+IVEACE FG FK++NHGVSD AIA LE 
Sbjct: 1   MPCPTAMRTKKAKAVGIPTIDLSESRARTSKAIVEACEEFGFFKLVNHGVSDQAIAGLER 60

Query: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           Q FDFF + APEKRRAGPA PFGYGCK IGPNGD G+LEY+LLRSDPA+IAETS SIS+ 
Sbjct: 61  QGFDFFAKPAPEKRRAGPADPFGYGCKTIGPNGDTGELEYLLLRSDPAAIAETSLSISSD 120

Query: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSY VNEY+KSV+ELACEILDLVGEGLWLPNKS+ S LIRDVQSDSVLRIN+YPA V
Sbjct: 121 PPSFSYAVNEYIKSVKELACEILDLVGEGLWLPNKSIISGLIRDVQSDSVLRINHYPA-V 180

Query: 181 KHTEKSRELSSKSHLSTN-KIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPS 240
           KHTE   ELSSKSH S N +IGFGEHSDPQILTILRSNDV GLQISL  GLW PVCPDPS
Sbjct: 181 KHTE-DWELSSKSHPSNNTRIGFGEHSDPQILTILRSNDVGGLQISLPGGLWVPVCPDPS 240

Query: 241 AFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKD 300
           AFYVLIGDAFQAM+N RFVSVRHRAMANNS K RMS+MYFGAPPLNAWIAP S++ML   
Sbjct: 241 AFYVLIGDAFQAMSNGRFVSVRHRAMANNSNKPRMSMMYFGAPPLNAWIAPLSELMLPHH 300

Query: 301 QSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
            S+Y+PFTW QYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 PSMYQPFTWGQYKKAAYSLRLADSRLDLFKAS 330

[Close]

BLAST of Csor.00g005350 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 169/327 (51.68%), Postives = 216/327 (66.06%), Query Frame = 0


Query: 5   TAMRSKKTKAVGIPTIDLS-ESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAF 64
           T  ++  +     P ID S   R++ S+ IV+ACE  G FKVINHGV    I   EH+  
Sbjct: 12  TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71

Query: 65  DFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS-NSPL 124
           +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LL ++P ++A+ S +IS + P 
Sbjct: 72  EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131

Query: 125 GFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKH 184
            FS   N+Y+++VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP A   
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA--- 191

Query: 185 TEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFY 244
                     +     +IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP+ F+
Sbjct: 192 --------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFF 251

Query: 245 VLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSL 304
           VL+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+  ++   
Sbjct: 252 VLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRR 311

Query: 305 YKPFTWAQYKKAAYSLRLADSRLNLFK 330
           Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 312 YNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of Csor.00g005350 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 165/326 (50.61%), Postives = 218/326 (66.87%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+ACE  G FKVINHGV    I+ +E ++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPAT-PFGYGCKAIGPNGDIGDLEYILLR-SDPASIAETSASISNSPLGF 128
            + A EK+   P   PF YG + IG NGD G++EY+L   +DPA           S L F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y+++V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVL 248
           ++  LS +S +S  ++GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF V 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++T +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLNLFK 330
           + FTW +YKK AYSLRL DSRL++F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of Csor.00g005350 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 146/314 (46.50%), Postives = 209/314 (66.56%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+ACE FG FKV+NHGV    +  LE +A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPATPFGYGCKAIGPNGDIGDLEYILLRSDP-ASIAETSASISNSPLGFSYVVNEYVKSV 136
           GP  P+GYG K IGPNGD+G +EY+LL ++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK          
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEK---------- 210

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
              K+GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F++ +GDA Q MTN 
Sbjct: 211 -MVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLNLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of Csor.00g005350 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 147/320 (45.94%), Postives = 205/320 (64.06%), Query Frame = 0


Query: 11  KTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTA 70
           K + V IP IDL++S A+T   IV+ACE FG FKVINHGV    +  LE +A +FF    
Sbjct: 21  KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80

Query: 71  PEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPA-SIAETSASISNSPLGFSYVVN 130
             K +AGP  PFGYG K IGPNGD+G LEYILL ++      +T+A   ++P  F   V 
Sbjct: 81  SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140

Query: 131 EYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSREL 190
           EY+K ++ ++ + L++V E L +  K   S L++  +SDS LR+N+YP   +   K    
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVK---- 200

Query: 191 SSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAF 250
                    +IGFGEH+DPQ++++LRSND +GLQI + DG W  V PD S+F+VL+GD  
Sbjct: 201 --------EEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTL 260

Query: 251 QAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWA 310
           Q MTN RF SV+HR +  N+K++R+S++YF  PPL+  IAP S ++  +D  LY  FTW+
Sbjct: 261 QVMTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWS 320

Query: 311 QYKKAAYSLRLADSRLNLFK 330
           QYK +AY  +L D RL LF+
Sbjct: 321 QYKLSAYKTKLGDYRLGLFE 325

[Close]

BLAST of Csor.00g005350 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 260.0 bits (663), Expect = 2.6e-69
Identity = 133/314 (42.36%), Postives = 200/314 (63.69%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEHQAFDFFGQTAPEKRRA 76
           IP ID+S+  ++   ++V+ACE FG FKVINHGVS   ++ LEH+  DFF     EK + 
Sbjct: 18  IPVIDMSDPESK--HALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77

Query: 77  GPATPFGYGCKAIGPNGDIGDLEYILLRSD-PASIAETSASISNSPLGFSYVVNEYVKSV 136
               PFGYG   IG NGD+G +EY+L+ ++  +       S+  SP  F   + EY  SV
Sbjct: 78  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 137

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           R++  ++L+ + +GL +  ++  S L+ D  +DS+LR+N+YP      +K+         
Sbjct: 138 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNG------- 197

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
             N IGFGEH+DPQI+++LRSN+  GLQI+L+DG W  V PD ++F+  +GD+ Q MTN 
Sbjct: 198 GKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNG 257

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SVRHR +A N KK+R+S++YF  P L   IAP + ++  +D+ LY+ FTW++YK + 
Sbjct: 258 RFKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNST 317

Query: 317 YSLRLADSRLNLFK 330
           Y+ RL+D+RL  F+
Sbjct: 318 YNSRLSDNRLQQFE 320

The following BLAST results are available for this feature:

BLAST of Csor.00g005350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHM5	1.2e-108	58.31	Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1	[more]
Q9FZ21	2.0e-90	51.68	Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ...	[more]
Q9C7Z1	2.3e-78	50.61	Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ...	[more]
Q9XFR9	2.5e-77	46.50	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9XG83	1.3e-76	46.37	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]

BLAST of Csor.00g005350 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593256.1	1.25e-242	100.00	Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
KAG7025608.1	1.70e-240	99.40	Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022959634.1	6.91e-240	99.09	gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata]	[more]
XP_023514766.1	2.31e-238	98.19	gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004594.1	2.13e-234	96.68	gibberellin 2-beta-dioxygenase 2 [Cucurbita maxima]	[more]

BLAST of Csor.00g005350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8N0	3.35e-240	99.09	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460...	[more]
A0A6J1KWQ4	1.03e-234	96.68	gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=...	[more]
A0A6J1GRC3	2.93e-197	83.69	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1JXB5	3.97e-195	83.08	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148834...	[more]
A0A6J1DEJ9	9.58e-192	81.63	gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 ...	[more]

BLAST of Csor.00g005350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02400.1	1.4e-91	51.68	gibberellin 2-oxidase 6	[more]
AT1G47990.1	1.6e-79	50.61	gibberellin 2-oxidase 4	[more]
AT1G30040.1	1.8e-78	46.50	gibberellin 2-oxidase	[more]
AT2G34555.1	2.4e-75	45.94	gibberellin 2-oxidase 3	[more]
AT1G78440.1	2.6e-69	42.36	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 33..50 score: 45.45 coord: 241..267 score: 27.27 coord: 205..226 score: 32.23
None	No IPR available	PANTHER	PTHR47990:SF45	GIBBERELLIN 2-BETA-DIOXYGENASE 6	coord: 4..329
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..321
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 168..280 e-value: 2.6E-21 score: 76.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..319 e-value: 1.0E-67 score: 230.8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 17..85 e-value: 4.1E-13 score: 50.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..282 score: 12.229617

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g005350.m01	Csor.00g005350.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding