CsaV3_7G030640 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_7G030640
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionCytochrome P450 protein
Locationchr7: 19392733 .. 19395524 (-)
RNA-Seq ExpressionCsaV3_7G030640
SyntenyCsaV3_7G030640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACAATTAAAAAAAGAATAAAAGATTGTTTGGAAAACAATTCTTTTGTTTTAATAATTTCAAAATTTATCTTTTGGAAATGACACTTTCCATTTTTTAGAATAATTCAAAGTTGTTTTGTGTTGTTGGCTTAATACAATCTACACCCGATTAGTAATTCTTCAAAATGATTTTGGAAACCAAAACTTAATATATAATAATTTTAAAAGAAACACCAAAGTTAGTTTATTTAGGCCATCTTCCTCTCCCAAGCAACTAAGAAACTTAAGCATTTTCAGCTCACCAAATTGAAACACTTTTCTTTTAGCCAAAAACAAGTTAAAAACAAGAAAGGCCAAAATTGCATGCTTTTTGCTGCCTTATATTGCATGACTATTCAACTTATTCTCCACTTTTTCACAACAATAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATCATGGATTCTGGAAACCTCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTTGGGCTCCGGTCGCTCCTCGATGTCCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATTGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTCCAATTTCGAGGTCCGTGGCTTACTAGTCTCAACTGCGTTGTCACGGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCTTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAAGCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTATTTTCTTTTTCTAATTTTGCATATTCAAATAGTTAATCCTTTTTTGTTTGAAAATAGTTTATTGTAGAGCTGCCCACCAAAACCTAACTAATCAGTTAAGATATTACTAGTAATCAATGTCAATAAAATTTTGACTCCATACTTCTACAGCAACAAAGTATTGGTTAAATTGTACTTAGAAAAAGGGCTAAATTACAGCTTTGGTAAATTATAACCATAGCTAAATTACCATTGAACTATACTAAGCTATTACTTTCAAATTTTGTAACATTATCTACAACTTTTTAAAAAATACTCTCAGAATAGATGTCATTTAAGAACATCCCCAATGATCATTTTCTTTTCCTAAGTCATTGTTGCACCTTTTTCAAATCACAAATTAAAAAAATATTTCTTATAATTTAGGGTGGAAAAAGCCTATCATTTAGTTTTGAGACAACAAAGTGTAAATAGATTATTTTGAGTGTAATGTGTAATTTCATAAATATATATCTATGTATCAAATTGTTATTCAGGTATTATTCTTATTTTAAAAAAACCCAATTTTTTTTAATTAATTTAAATTTAAGCAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGACGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAGAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTAATTATAACAATTACTCATTCACATTCTAATTTCGAAAGAAAACTTCAGAAATCTAATCGATAATCCGTCTGATTCAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACCGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGATTAGTGGGAGTGTTAATAAATT

mRNA sequence

ATGCTTTTTGCTGCCTTATATTGCATGACTATTCAACTTATTCTCCACTTTTTCACAACAATAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATCATGGATTCTGGAAACCTCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTTGGGCTCCGGTCGCTCCTCGATGTCCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATTGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTCCAATTTCGAGGTCCGTGGCTTACTAGTCTCAACTGCGTTGTCACGGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCTTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAAGCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGACGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAGAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACCGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGA

Coding sequence (CDS)

ATGCTTTTTGCTGCCTTATATTGCATGACTATTCAACTTATTCTCCACTTTTTCACAACAATAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATCATGGATTCTGGAAACCTCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTTGGGCTCCGGTCGCTCCTCGATGTCCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATTGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTCCAATTTCGAGGTCCGTGGCTTACTAGTCTCAACTGCGTTGTCACGGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCTTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAAGCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGACGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAGAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACCGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGA

Protein sequence

MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN*
Homology
BLAST of CsaV3_7G030640 vs. NCBI nr
Match: XP_004135954.3 (cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_015511 [Cucumis sativus])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG 60
           MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG
Sbjct: 1   MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG 60

Query: 61  HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120
           HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI
Sbjct: 61  HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180
           TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI 300
           LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI 300

Query: 301 GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD 360
           GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF 420

Query: 421 DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY 480
           DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY
Sbjct: 421 DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 590
           SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN
Sbjct: 541 SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 589

BLAST of CsaV3_7G030640 vs. NCBI nr
Match: XP_008461361.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 557/589 (94.57%), Postives = 572/589 (97.11%), Query Frame = 0

Query: 1   MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG 60
           MLFAALYCMTIQLILHFFTT TSSMFSHAFPFLHHKPIMDSGNL SSSSSSS  P IS+ 
Sbjct: 1   MLFAALYCMTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNL-SSSSSSSLPPSISVR 60

Query: 61  HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120
           HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI
Sbjct: 61  HFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180
           TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HSRLEAVLEDF G++TTVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI 300
           LLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVTV+RFITPTFIWK MRYLNI
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNI 300

Query: 301 GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD 360
           GIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+RSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKIL EICRIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRF 420

Query: 421 DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY 480
           DSLVFKPEE K+MEYLHAAISEALRLYPSVPFDHKEVVEDD FPDGT+LKKG KIIYAIY
Sbjct: 421 DSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 590
           SIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 SIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of CsaV3_7G030640 vs. NCBI nr
Match: KAA0058578.1 (cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450 86B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.8e-302
Identity = 522/551 (94.74%), Postives = 537/551 (97.46%), Query Frame = 0

Query: 39  MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 98
           MDSGNL SSSSSSS  P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPI
Sbjct: 1   MDSGNL-SSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPI 60

Query: 99  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158
           WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 159 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 219 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 278
           SRLEAVLEDF G++TTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT 240

Query: 279 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 338
           EVTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N
Sbjct: 241 EVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADN 300

Query: 339 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 398
           +RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDK
Sbjct: 301 KRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDK 360

Query: 399 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 458
           IL EICRIVGEREEAKTRELRFDSLVFKPEEIK+MEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 361 ILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 459 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 518
           EDD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAF
Sbjct: 421 EDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAF 480

Query: 519 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 578
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 540

Query: 579 EGQLRKYIKLN 590
           EGQ+RKY+KLN
Sbjct: 541 EGQIRKYMKLN 550

BLAST of CsaV3_7G030640 vs. NCBI nr
Match: KAG7014270.1 (Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.2e-294
Identity = 505/567 (89.07%), Postives = 535/567 (94.36%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPP--ISIGHFGLRSLLDVQILEITFALFVF 84
           MFSHA PFLHHKP+MDSGNLS+SS     +PP  +S G+FGL  LLD QILEI+FALF+F
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSTSS-----APPALVSAGNFGLWPLLDFQILEISFALFMF 60

Query: 85  LVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLN 144
           L IHVVRQ+RR+GLP+WPIVGMLP+LITA RGNLYEWITDLL RHKGTFQFRGPW +SLN
Sbjct: 61  LAIHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLN 120

Query: 145 CVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHS 204
           CVVTADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHS
Sbjct: 121 CVVTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHS 180

Query: 205 ARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLR 264
           ARFRQMTVESLVELVHSRLE VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR
Sbjct: 181 ARFRQMTVESLVELVHSRLETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLR 240

Query: 265 SGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQ 324
            GLPEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQ
Sbjct: 241 GGLPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQ 300

Query: 325 TRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL 384
           TRKRELST A+ QAENQRSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL
Sbjct: 301 TRKRELSTTAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL 360

Query: 385 SWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISE 444
           SWFF LLD+NPDVEDKIL EI RIVGEREEAKTRE RFDSLVFKPEEIK+MEYLHAAISE
Sbjct: 361 SWFFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISE 420

Query: 445 ALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM 504
           ALRLYPSVP DHKEVVEDDVFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM
Sbjct: 421 ALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM 480

Query: 505 REGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLAL 564
           ++GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLAL
Sbjct: 481 KDGRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLAL 540

Query: 565 TFYMKKGLKVNLIKREEGQLRKYIKLN 590
           TFYMK GLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 TFYMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of CsaV3_7G030640 vs. NCBI nr
Match: XP_022954102.1 (cytochrome P450 86B1-like [Cucurbita moschata])

HSP 1 Score: 1017.7 bits (2630), Expect = 4.1e-293
Identity = 503/565 (89.03%), Postives = 532/565 (94.16%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLV 84
           MFSHA PFLHHKP+MDSGNLSSSS+  +    +S G+FGL  LLD QILEI+FALFVFL 
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSSALPAL---VSAGNFGLWPLLDFQILEISFALFVFLA 60

Query: 85  IHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCV 144
           IHVVRQ+RR+GLP+WPIVGMLP+LITA RGNLYEWITDLL  HKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSG 264
           FRQMTVESLVELVHSRLE VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR G
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTR 324
           LPEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQTR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTR 300

Query: 325 KRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST A+ QAENQRSDLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEAL 444
           FF LLD+NPDVEDKIL EI RIVGEREEAKTRE RFDSLVFKPEEIK+MEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRE 504
           RLYPSVP DHKEVVEDDVFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKKGLKVNLIKREEGQLRKYIKLN 590
           YMK GLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of CsaV3_7G030640 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.8e-220
Identity = 375/538 (69.70%), Postives = 440/538 (81.78%), Query Frame = 0

Query: 48  SSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPA 107
           SSSSS  P +S   F LR   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 108 LITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGS 167
           L    RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 168 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLED 227
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 228 FEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFIT 287
               ++ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 288 PTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVF 347
           PT +WKFMRYL+IG EKKLK+SIK VD+FA++VI+TRK+ELS   E+    +RSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVF 316

Query: 348 MKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIV 407
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFF LL++NP+VE+KI+ E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 408 GEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGT 467
            +R++    E      VF PEEIK+M+YL AA+SEALRLYPSVP DHKEV EDDVFPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 468 VLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLG 527
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+R+GRFMSESAY+FTAFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 528 KDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKY 586
           KDFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK GL VNLI R   ++ +Y
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of CsaV3_7G030640 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.3e-128
Identity = 244/517 (47.20%), Postives = 336/517 (64.99%), Query Frame = 0

Query: 68  LDVQILEITFALFVFLVIHVVRQKRRH-GLPIWPIVGMLPALITATRGNLYEWITDLLCR 127
           +D     I   L V  +I  V  +R + G  +WP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 128 HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 187
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 188 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVL 247
           F +D +TW+ QRK A++EF +   RQ     +   + +RL  +LE    +A  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 248 LRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIG 307
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P FIWK  ++L +G
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 308 IEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDK 367
           +E  + +SI  VD +  ++I TRK EL    + Q E++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLELL--GQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 368 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 427
           +L+ + +NFILAGRDTSSVA+SWFF L+  NP VE+KI+ EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 428 SLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 487
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 488 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 547
           +GRM+ IWG+DC EFKPERW+ E R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 548 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 579
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of CsaV3_7G030640 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.2e-125
Identity = 247/516 (47.87%), Postives = 326/516 (63.18%), Query Frame = 0

Query: 70  VQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGN-LYEWITDLLCRHK 129
           + I+ I  A  ++        K   G  +WP+VG LP LI    GN ++EWI D L    
Sbjct: 7   MMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLI--ENGNRMHEWIADNLRACS 66

Query: 130 GTFQ---FRGPWLTSLNCV-VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGA 189
           GT+Q      P+L     V VT DP+NLEH+LK +F +YPKG  ++    DLLG+GIF +
Sbjct: 67  GTYQTCICAIPFLARKQLVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNS 126

Query: 190 DDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRL 249
           D +TW  QRK A++EF +   RQ     +   + +R   +LE  + Q   VDLQD+LLRL
Sbjct: 127 DGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRL 186

Query: 250 TFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEK 309
           TFDN+C +AFG DP  L   LPE  FA +F+ ATE T+ RFI P F+WK  + L +G+E 
Sbjct: 187 TFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEV 246

Query: 310 KLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLR 369
            L  S+K VD +  DVI TRK EL          Q  DLL+ FMK ++     +SDKFL+
Sbjct: 247 SLNNSLKQVDNYMTDVINTRKLELLN--HQNGGPQHDDLLSRFMKKKES----YSDKFLQ 306

Query: 370 DICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLV 429
            + +NFILAGRDTSSVALSWFF L+  NP VE+KIL EIC I+ E     T +   + LV
Sbjct: 307 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 366

Query: 430 FKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGR 489
           F  EE+ ++ YL AA+SE LRLYPSVP D K V+ DD  PDGT +  G+ I Y+IY+ GR
Sbjct: 367 F--EEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGR 426

Query: 490 MEAIWGKDCREFKPERWMRE--GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASI 549
           M+ IWG+DC EFKPERWM +   +F  +  +RF AFN GPR+CLGKD AY QMK +AA++
Sbjct: 427 MKFIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAV 486

Query: 550 ISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 579
           + R+R+AV  GH VE K++LT +MK GL +N+  R+
Sbjct: 487 LLRHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRD 512

BLAST of CsaV3_7G030640 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.6e-125
Identity = 239/492 (48.58%), Postives = 318/492 (64.63%), Query Frame = 0

Query: 95  GLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQ---FRGPWLTSLN--CVVTADP 154
           G  +WP++G LP LI   R  ++EWIT+ L    GT+Q   F  P+L        VT DP
Sbjct: 29  GPRVWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDP 88

Query: 155 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 214
           +NLEH+LKT+F +YPKG  ++    DLLG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 215 VESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIP 274
              +   +  R   +L   +  A  VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 275 FAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELS 334
           FA AF+ ATE ++ RFI P F+WK  ++L +G+E  L +S+  +DE+   VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQELM 268

Query: 335 TPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLL 394
           +  ES    +  DLL+ FM  + E    +SD FL+ + +NFILAGRDTSSVALSWFF L+
Sbjct: 269 SQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFWLI 328

Query: 395 DQNPDVEDKILEEICRIVGE-REEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYP 454
             +P VEDKI+ EIC ++ E R          + L F  +EI R+ YL AAISE LRLYP
Sbjct: 329 TMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGF--DEIDRLVYLKAAISETLRLYP 388

Query: 455 SVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--EGR 514
           SVP D K V  DDV PDGT +  G+ + Y+IYA GRM++ WG+DC EF PERW+   +G+
Sbjct: 389 SVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWISPIDGK 448

Query: 515 FMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYM 574
           F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ VV GH VE K++LT +M
Sbjct: 449 FINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVVPGHKVEQKMSLTLFM 508

Query: 575 KKGLKVNLIKRE 579
           K GL VNL KR+
Sbjct: 509 KNGLLVNLYKRD 514

BLAST of CsaV3_7G030640 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.9e-123
Identity = 230/491 (46.84%), Postives = 319/491 (64.97%), Query Frame = 0

Query: 95  GLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQ---FRGPWLTSLN--CVVTADP 154
           G  +WP++G LP LI   R  +++WIT+ L    GT+Q      P+L        VT DP
Sbjct: 29  GPRVWPVLGSLPGLI-EQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDP 88

Query: 155 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 214
           +N+EH+LKT+F +YPKG  ++    D LG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 215 VESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIP 274
              +   +  R   +LE  +     VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 275 FAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELS 334
           FA AF+ ATE ++ RFI P F+W+  ++L +G+E  L +S+  +D + + VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQELL 268

Query: 335 TPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLL 394
           +  ES  + +  DLL+ FMK +D+    +S+ FLR + +NFILAGRDTSSVALSWFF L+
Sbjct: 269 SQRESGVQ-RHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWLI 328

Query: 395 DQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPS 454
             +P VEDKI+ EIC ++ E           + L F  +E+ R+ YL AA+SE LRLYPS
Sbjct: 329 TTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEF--DEVDRLVYLKAALSETLRLYPS 388

Query: 455 VPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--EGRF 514
           VP D K VV DD+ PDGT +  G+ + Y+IYA GRM++ WG+DC EFKPERW+   +G+F
Sbjct: 389 VPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKF 448

Query: 515 MSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMK 574
           ++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ V  GH VE K++LT +MK
Sbjct: 449 VNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMK 508

Query: 575 KGLKVNLIKRE 579
            GL VN+ KR+
Sbjct: 509 NGLLVNVHKRD 512

BLAST of CsaV3_7G030640 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 557/589 (94.57%), Postives = 572/589 (97.11%), Query Frame = 0

Query: 1   MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG 60
           MLFAALYCMTIQLILHFFTT TSSMFSHAFPFLHHKPIMDSGNL SSSSSSS  P IS+ 
Sbjct: 1   MLFAALYCMTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNL-SSSSSSSLPPSISVR 60

Query: 61  HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120
           HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI
Sbjct: 61  HFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180
           TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HSRLEAVLEDF G++TTVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI 300
           LLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVTV+RFITPTFIWK MRYLNI
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNI 300

Query: 301 GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD 360
           GIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+RSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKIL EICRIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRF 420

Query: 421 DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY 480
           DSLVFKPEE K+MEYLHAAISEALRLYPSVPFDHKEVVEDD FPDGT+LKKG KIIYAIY
Sbjct: 421 DSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 590
           SIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 SIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of CsaV3_7G030640 vs. ExPASy TrEMBL
Match: A0A0A0K879 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 39  MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 98
           MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI
Sbjct: 1   MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60

Query: 99  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158
           WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 159 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 219 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 278
           SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 240

Query: 279 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 338
           EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN
Sbjct: 241 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 300

Query: 339 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 398
           QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK
Sbjct: 301 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 360

Query: 399 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 458
           ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 361 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 459 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 518
           EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF
Sbjct: 421 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 480

Query: 519 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 578
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 540

Query: 579 EGQLRKYIKLN 590
           EGQLRKYIKLN
Sbjct: 541 EGQLRKYIKLN 551

BLAST of CsaV3_7G030640 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.4e-302
Identity = 522/551 (94.74%), Postives = 537/551 (97.46%), Query Frame = 0

Query: 39  MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 98
           MDSGNL SSSSSSS  P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPI
Sbjct: 1   MDSGNL-SSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPI 60

Query: 99  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158
           WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 159 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 219 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 278
           SRLEAVLEDF G++TTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT 240

Query: 279 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 338
           EVTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N
Sbjct: 241 EVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADN 300

Query: 339 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 398
           +RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDK
Sbjct: 301 KRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDK 360

Query: 399 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 458
           IL EICRIVGEREEAKTRELRFDSLVFKPEEIK+MEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 361 ILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 459 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 518
           EDD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAF
Sbjct: 421 EDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAF 480

Query: 519 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 578
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 540

Query: 579 EGQLRKYIKLN 590
           EGQ+RKY+KLN
Sbjct: 541 EGQIRKYMKLN 550

BLAST of CsaV3_7G030640 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 2.0e-293
Identity = 503/565 (89.03%), Postives = 532/565 (94.16%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLV 84
           MFSHA PFLHHKP+MDSGNLSSSS+  +    +S G+FGL  LLD QILEI+FALFVFL 
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSSALPAL---VSAGNFGLWPLLDFQILEISFALFVFLA 60

Query: 85  IHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCV 144
           IHVVRQ+RR+GLP+WPIVGMLP+LITA RGNLYEWITDLL  HKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSG 264
           FRQMTVESLVELVHSRLE VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR G
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTR 324
           LPEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQTR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTR 300

Query: 325 KRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST A+ QAENQRSDLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEAL 444
           FF LLD+NPDVEDKIL EI RIVGEREEAKTRE RFDSLVFKPEEIK+MEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRE 504
           RLYPSVP DHKEVVEDDVFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKKGLKVNLIKREEGQLRKYIKLN 590
           YMK GLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of CsaV3_7G030640 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.9e-290
Identity = 499/565 (88.32%), Postives = 528/565 (93.45%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLV 84
           MFSHA PFLHHKPIMDSGNLSSSS+  +    +S  +F L  LLDVQILE++FALFVFL 
Sbjct: 1   MFSHAIPFLHHKPIMDSGNLSSSSALPAV---VSAENFRLWPLLDVQILELSFALFVFLA 60

Query: 85  IHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCV 144
           IHV+RQ+RR+GL +WPIVGMLP+LITA RGNLYEWITDLL RHKGTFQFRGPW +SLNCV
Sbjct: 61  IHVIRQQRRYGLSVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF  YPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPYYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSG 264
           FRQMTVESLVELVHSRLE VLEDF  + T VDLQDVLLRLTFDNVCMIAFGVDPGCLR G
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFVERRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTR 324
           LPEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSIK VDEFAEDVIQTR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIKTVDEFAEDVIQTR 300

Query: 325 KRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST A+ QA NQRSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQANNQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEAL 444
           FF LLDQNPDVE+KIL EI RIVGER EAKTRE RFDSLVFKPEEIK+MEYLHAAISEAL
Sbjct: 361 FFWLLDQNPDVENKILAEIYRIVGERGEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRE 504
           RLYPSVP DHKEVVEDDVFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKKGLKVNLIKREEGQLRKYIKLN 590
           YMK GLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYMKLN 562

BLAST of CsaV3_7G030640 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 766.1 bits (1977), Expect = 2.0e-221
Identity = 375/538 (69.70%), Postives = 440/538 (81.78%), Query Frame = 0

Query: 48  SSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPA 107
           SSSSS  P +S   F LR   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 108 LITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGS 167
           L    RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 168 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLED 227
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 228 FEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFIT 287
               ++ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 288 PTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVF 347
           PT +WKFMRYL+IG EKKLK+SIK VD+FA++VI+TRK+ELS   E+    +RSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVF 316

Query: 348 MKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIV 407
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFF LL++NP+VE+KI+ E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 408 GEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGT 467
            +R++    E      VF PEEIK+M+YL AA+SEALRLYPSVP DHKEV EDDVFPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 468 VLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLG 527
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+R+GRFMSESAY+FTAFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 528 KDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKY 586
           KDFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK GL VNLI R   ++ +Y
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of CsaV3_7G030640 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 714.1 bits (1842), Expect = 9.0e-206
Identity = 343/493 (69.57%), Postives = 412/493 (83.57%), Query Frame = 0

Query: 96  LPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHL 155
           +P+WP+VGMLP+LI+A R N+YEW++D+L    GTF+FRGPW ++LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 156 LKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVE 215
           LKT+FS YPKGS+FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSA+FRQ+T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 216 LVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFE 275
           LVH+RL  VLE     +  +DLQD+LLRLTFDNVCMIAFGVDPGCL   LPEIPFAKAFE
Sbjct: 121 LVHNRLLPVLE----TSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFE 180

Query: 276 DATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQ 335
           DATE TV+RF+ P F+WK MR LN+G EKKLK+SI  VD+FAE+VI+TRK+E+S   E  
Sbjct: 181 DATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEIA 240

Query: 336 AENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDV 395
              +R DLLT+FM LRDE G+ FSDKFLRDICVNFILAGRDTSSVALSWFF L+++NP+V
Sbjct: 241 ---KRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEV 300

Query: 396 EDKILEEICRIVGER-EEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDH 455
           E+KI+  IC+I+ +R +   T++      VF+PEEIK+M+YL AA+SE LRLYPSVP DH
Sbjct: 301 EEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDH 360

Query: 456 KEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYR 515
           KEV+EDDVFPDGT LKKG K+IYAIYAMGRME IWGKDCREFKPERW+R+GR+MSESAY+
Sbjct: 361 KEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYK 420

Query: 516 FTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAV--VRGHPVEPKLALTFYMKKGLKV 575
           FTAFNGGPRLCLGKDFAYYQM+YVAA+II RY+V V    GH VEPK+ALT YMK GLKV
Sbjct: 421 FTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTMYMKHGLKV 480

Query: 576 NLIKREEGQLRKY 586
           N++KR   ++  Y
Sbjct: 481 NMVKRSVSEIDHY 486

BLAST of CsaV3_7G030640 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 492.7 bits (1267), Expect = 4.2e-139
Identity = 242/517 (46.81%), Postives = 347/517 (67.12%), Query Frame = 0

Query: 70  VQILEITFALFVFLVIHVVRQK--RRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRH 129
           +++ ++  AL    ++  +R+K   + G  +WP++G++P ++   + +L+ W T  + R 
Sbjct: 15  LRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCTRCVVRS 74

Query: 130 KGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDE 189
            GTF +RG W      ++TADP N+EH+LKT F +YPKG+F+R+  RDLL DGIF ADDE
Sbjct: 75  GGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDE 134

Query: 190 TWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFD 249
            W+ +R+VA  E HS+RF + T  ++ +LV  +L  ++E+        DLQD+LLR TFD
Sbjct: 135 LWKEERRVAKTEMHSSRFLEHTFTTMRDLVDQKLVPLMENLSTSKRVFDLQDLLLRFTFD 194

Query: 250 NVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLK 309
           N+C+ AFGV PG L +GLPEIPFAKAFEDATE T+ RF+ P F+WK MR+L IG E+KL 
Sbjct: 195 NICISAFGVYPGSLETGLPEIPFAKAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLN 254

Query: 310 QSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDE------QGRPFSDK 369
            +++ V  FA   ++ R+ ++         N  +DLL+  M+   E      +G  FSDK
Sbjct: 255 NAVRIVHAFANKTVRERRNKMRKLGNL---NDYADLLSRLMQREYEKEEDTTRGNYFSDK 314

Query: 370 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 429
           + R+ C +FI+AGRDT+SVAL WFF L+ ++P+VE +IL EI  I  +    +T +    
Sbjct: 315 YFREFCTSFIIAGRDTTSVALVWFFWLVQKHPEVEKRILREIREIKRKLTTQETED---- 374

Query: 430 SLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 489
              F+ E+ + M YL AA++E+LRLYPSVP + K+ +EDDV PDGT +KKG +I Y++Y+
Sbjct: 375 --QFEAEDFREMVYLQAALTESLRLYPSVPMEMKQALEDDVLPDGTRVKKGARIHYSVYS 434

Query: 490 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 549
           MGR+E+IWGKD  EFKPERW++EGR +SE  +++  FNGGPRLC+GK FAY QMK VAA+
Sbjct: 435 MGRIESIWGKDWEEFKPERWIKEGRIVSEDQFKYVVFNGGPRLCVGKKFAYTQMKMVAAA 494

Query: 550 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 579
           I+ RY V VV+G  + PKL  T YMK G+ V L  R+
Sbjct: 495 ILMRYSVKVVQGQEIVPKLTTTLYMKNGMNVMLQPRD 521

BLAST of CsaV3_7G030640 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 465.3 bits (1196), Expect = 7.3e-131
Identity = 227/507 (44.77%), Postives = 334/507 (65.88%), Query Frame = 0

Query: 74  EITFALFVFLVIHVVRQK--RRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTF 133
           +++ AL    V   VR+K  ++ G  IWP+ G+ P      R ++Y W T  L + +GTF
Sbjct: 22  DVSLALLGLFVFCCVREKVTKKLGPTIWPVFGITPEFF-FHRNDVYGWATRCLKKCRGTF 81

Query: 134 QFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQR 193
            + G WL      VT  P N+E++LKT F ++PKG+FF++   DLL DGIF AD E+W+ 
Sbjct: 82  LYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKE 141

Query: 194 QRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCM 253
           QR++   E HS RF + + ++  +LV  +L  V+E F       DLQDVLLRLTFDN+C+
Sbjct: 142 QRRIIITEMHSTRFVEHSFQTTQDLVRKKLLKVMESFARSQEAFDLQDVLLRLTFDNICI 201

Query: 254 IAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIK 313
              G DPG L S LP +PFA+AFE+ATE T+ RF+ P FIWK +++ +IG EK L++++ 
Sbjct: 202 AGLGDDPGTLDSDLPLVPFAQAFEEATESTMFRFMIPPFIWKPLKFFDIGYEKGLRKAVD 261

Query: 314 AVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRD----EQGRPFSDKFLRDIC 373
            V EF + ++  R  +L    E      RSD+L+  +++      ++  P + KF R  C
Sbjct: 262 VVHEFVDKMVVDRICKLK---EEGTLGNRSDVLSRIIEIESHKTTDEKDPSTIKFFRQFC 321

Query: 374 VNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKP 433
            +FILAGRDTSSVAL+WFF ++ ++P+VE+KI+ EI  I+ +R ++ T +   +  +F  
Sbjct: 322 TSFILAGRDTSSVALTWFFWVIQKHPEVENKIIREISEILRQRGDSPTSK---NESLFTV 381

Query: 434 EEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEA 493
           +E+  M YL AA+SE +RLYP +P + K+ +EDDVFPDGT ++KG+++ +A YAMGRME+
Sbjct: 382 KELNDMVYLQAALSETMRLYPPIPMEMKQAIEDDVFPDGTFIRKGSRVYFATYAMGRMES 441

Query: 494 IWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYR 553
           IWGKDC  FKPERW++ G F+++  +++  FN GPRLCLGK FAY QMK +AAS++SRY 
Sbjct: 442 IWGKDCESFKPERWIQSGNFVNDDQFKYVVFNAGPRLCLGKTFAYLQMKTIAASVLSRYS 501

Query: 554 VAVVRGHPVEPKLALTFYMKKGLKVNL 575
           + V + H V P++  T YM+ GLKV +
Sbjct: 502 IKVAKDHVVVPRVTTTLYMRHGLKVTI 521

BLAST of CsaV3_7G030640 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 459.9 bits (1182), Expect = 3.0e-129
Identity = 244/517 (47.20%), Postives = 336/517 (64.99%), Query Frame = 0

Query: 68  LDVQILEITFALFVFLVIHVVRQKRRH-GLPIWPIVGMLPALITATRGNLYEWITDLLCR 127
           +D     I   L V  +I  V  +R + G  +WP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 128 HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 187
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 188 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVL 247
           F +D +TW+ QRK A++EF +   RQ     +   + +RL  +LE    +A  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 248 LRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIG 307
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P FIWK  ++L +G
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 308 IEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDK 367
           +E  + +SI  VD +  ++I TRK EL    + Q E++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLELL--GQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 368 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 427
           +L+ + +NFILAGRDTSSVA+SWFF L+  NP VE+KI+ EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 428 SLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 487
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 488 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 547
           +GRM+ IWG+DC EFKPERW+ E R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 548 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 579
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135954.30.0e+00100.00cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_01... [more]
XP_008461361.10.0e+0094.57PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo][more]
KAA0058578.12.8e-30294.74cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450... [more]
KAG7014270.12.2e-29489.07Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022954102.14.1e-29389.03cytochrome P450 86B1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FMY12.8e-22069.70Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD64.3e-12847.20Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
B3RFJ61.2e-12547.87Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9LMM12.6e-12548.58Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
O230661.9e-12346.84Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CEY80.0e+0094.57LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0K8790.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1[more]
A0A5A7UTT41.4e-30294.74Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1GQ362.0e-29389.03cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A6J1JMG15.9e-29088.32cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23190.12.0e-22169.70cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.19.0e-20669.57Cytochrome P450 superfamily protein [more]
AT1G24540.14.2e-13946.81cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.17.3e-13144.77cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT1G63710.13.0e-12947.20cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 437..455
score: 31.1
coord: 362..379
score: 28.15
coord: 525..548
score: 19.46
coord: 382..408
score: 36.53
coord: 515..525
score: 42.0
IPR001128Cytochrome P450PRINTSPR00385P450coord: 525..536
score: 35.08
coord: 438..449
score: 44.94
coord: 373..390
score: 39.78
coord: 516..525
score: 46.03
IPR001128Cytochrome P450PFAMPF00067p450coord: 96..562
e-value: 2.2E-79
score: 267.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 91..587
e-value: 1.4E-106
score: 358.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 95..577
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 60..578
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 60..578
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 518..527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G030640.1CsaV3_7G030640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen