CsaV3_7G026620 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_7G026620
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionAsparagine--tRNA ligase
Locationchr7: 16150458 .. 16155205 (+)
RNA-Seq ExpressionCsaV3_7G026620
SyntenyCsaV3_7G026620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTCTTTTGTCTTTTGTCTTTTTGTTGAGAAATTATTCTGTGATCCTGCCACAGTTTTCCATCATTGTATATTCTATGACATATCTGATGGTTGTTAGAGGCATACAAATTTTGAATTCACATCCAATGGTTCTTGCGAGACAGCTACACTATTGAATAATTTGACCTTTTTATGATTAAAATGACTTTTAATGTTTTTCCCCTGTATCTGTTTTAAAAAAAAAATTGTGTCGCATATGGATGAATATATGTTCTTCCCTAACCAAACAAGAAATATGGAACTTTTAACAGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTCTGATATTTTTTATTACAATGTGTTGGAGGATAGTCGGTTGCTTAAGCTTTGGTTGTGCTTGGTTTTTTGCATTCTCACAGTAATAAGAAACTTGTAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAGGGCGTATTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATCCTTCATGTAGGAAAGGTTGAACAGGAAAAGTACCCATTGTCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAATATGCATAAGCTTCAACCATACGTTCCATTTATTTCCTATTTTATGGTTCTGTTTGCATGCCTATAATTAGAGTGCCTATTAGCTTTAAGATCGTGCTTACTGCTTATGCAACAGCACCATGTGAATTAGAACAAAGCGTTCTGTTCATATTTTATATCTAGTGATTGCCTTGAGATTCGAGAACAGGAGATTTATACAGTTAACAGCTCATTTTCTGCTAAAAGAAAGAATTGATATATATAGTTCAACGTTCCTGATCATGGATTAAAGTAACTTCATTGTAAATCTCCTTGGTAGGATCGCTTGCATTGACCTTTATAGAACCTGTGCAATGGAATTTGGTGTCAAAGCTTGAGAAGAGAGTTTTGCATATCTAATCATTAGAACTAATGAATTGTTGGATCTTGATCTTCATTGGCATTCTTTACTTATGCAGGTAGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAACCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCCTAGTAACTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCCAAATCCAAATCAAGGAAAGATAAAGTTATTCCTGGAAATTTTTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTTTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGTATGGATTTGATCTTTTACTTATTTCTAAAGATGGCTACTTTATATTATGGGTTTAAGATTCCGTATTGATTTAGCCATTAAAATCCAATGTTATTTTCTGTGGTTAATACTTTATCATAATAACTTTGCACCATTGAAATGAAGATTTTCTATCTTTTAACTTGGATTTTTGTATCATCATTTGGAATCAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGATGATATGAAATTTGTTCAAAAACGCATTGACAAGAATAGCATCCATCGCCTCGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTACCGCAATTGAAATCCTGGAAAAGGTGAAATCTCATCTACTCTTCTATCATGTGACTCAGTTTTTCATTTCTACCCCTGCCCATCTGAATGTTGAGAAAAAAAAATCTTCACTGTCAGGCAACCAATCAAAAATCTCCACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGGTGTGCAAAATGTGCTATTACAGTCAGTCACTAAGCAATTTTTCGTTACAAATAAAAAGTTCTTTCTTCTTTGTACGTTAAACAGTTATCTGGCTGATACCATCTTTAAAAAGCCTGTAATAGTGTGTAACTACCCAAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGTTAGCACATGGGCATCTATTTACCCGTGGGAAAATTTATTTTTAGATGGATCCTGAATTGAATGAAAAATTATTTCTGAAATCTGGGTCCCAACTGTTTGCAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGTATGATATCTTACTGCTTTGATTACTATTCTGTTGATATTTTTCTTGTCTATTCCATTCCAGACTTCCAAATCTTCTAAAATTGTTTTTTAATAAATTATTCAACAGAGACTTACACATTGTCTGTCACTTCTTTTATTTATTTACTACATCAATTGATATCTACATAGGTTGGATGTTGGGGGATTCCATTATAGATTTTGGATTTTTTAAACAATTAATGAAATAAAATGGGAAGGTTGAAGATCAAGTAGGAAGATGAAATAGTTTGAGATCAAGATCAAAAGAAATTTTAACTTTCATGAATTCTTTTAAAAAAGAATACCAAAATTTTGCATTGAGGTAATGAAAAGAAACTAACGTTAGATAGGAAAAACTATAAGCAAAAATAAAAACATCAAAACTTAGAGGGATAGAAGTCCGAAAACTAAACATTCCAAAAGTGAGCTAGCCCAGGAGTAATTTGCATGATTTTTCATCTTAAAGCCTAAAGGTAAGAGGTTTTATCTTCATCCCCACAACTCAAAAAAAAGAAAAAAAAAGGACTATAAATGCCATTCCAGCTGCAATCTATATTCTTGAATTGAAAAGATTTAGTTTAACTATCATGAGTTCAAACCCAGCGATAGCTGGCTTGGGATAAAATCTTACTAGTTTGACAACACTACGCTCACGGATATTAGAAAGAAAAAATGTGAGAGTTATTGATATCTGTTTGAATATTGATGGTTGTTGGCAGGATGAGGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTATTTAGAGATTCAAAGGCATGGAATAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAAAGAGAAACCCAAGATGAAATTGTTCAAAAATGTTTCACAACGTTGCTGGCATCCGATCAATGGTGATGCTTGAAAAATGGAACTGAAAACTGTGTTTGTTACTGTAAGAAGTAAAGGGAAAATGACTTCTGTTACTAGAGGACTTGTGTTTGTTGCTTCCTAAGATTTGTTCTTGTAAATAAAATAGAATTTGGATTATTTGTTGAGAAGACATGATTCAAACTTGTGATCTCCAGTTCTTAATTGCTATTCTTAGCTTCAATCATTACGATTTCTTATTAAAATCTTATGCAAATCATTTTGTTTTAAATGCTTTGATTACATTAAATAGTGAAAAAAAAATAGCCATCAAGTAAGGCAAAAAG

mRNA sequence

ATGTTTTTCCCCTGTATCTGTTTTAAAAAAAAAATTGTGTCGCATATGGATGAATATATGTTCTTCCCTAACCAAACAAGAAATATGGAACTTTTAACAGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAGGGCGTATTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATCCTTCATGTAGGAAAGGTTGAACAGGAAAAGTACCCATTGTCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAACCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCCTAGTAACTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCCAAATCCAAATCAAGGAAAGATAAAGTTATTCCTGGAAATTTTTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTTTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGATGATATGAAATTTGTTCAAAAACGCATTGACAAGAATAGCATCCATCGCCTCGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTACCGCAATTGAAATCCTGGAAAAGGCAACCAATCAAAAATCTCCACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGTTATCTGGCTGATACCATCTTTAAAAAGCCTGTAATAGTGTGTAACTACCCAAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGATGAGGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTATTTAGAGATTCAAAGGCATGGAATAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAA

Coding sequence (CDS)

ATGTTTTTCCCCTGTATCTGTTTTAAAAAAAAAATTGTGTCGCATATGGATGAATATATGTTCTTCCCTAACCAAACAAGAAATATGGAACTTTTAACAGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAGGGCGTATTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATCCTTCATGTAGGAAAGGTTGAACAGGAAAAGTACCCATTGTCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAACCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCCTAGTAACTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCCAAATCCAAATCAAGGAAAGATAAAGTTATTCCTGGAAATTTTTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTTTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGATGATATGAAATTTGTTCAAAAACGCATTGACAAGAATAGCATCCATCGCCTCGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTACCGCAATTGAAATCCTGGAAAAGGCAACCAATCAAAAATCTCCACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGTTATCTGGCTGATACCATCTTTAAAAAGCCTGTAATAGTGTGTAACTACCCAAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGATGAGGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTATTTAGAGATTCAAAGGCATGGAATAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAA

Protein sequence

MFFPCICFKKKIVSHMDEYMFFPNQTRNMELLTEFKVDAELRKVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN*
Homology
BLAST of CsaV3_7G026620 vs. NCBI nr
Match: XP_031745191.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X2 [Cucumis sativus] >KAE8646266.1 hypothetical protein Csa_015513 [Cucumis sativus])

HSP 1 Score: 1036.9 bits (2680), Expect = 5.8e-299
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MFFPCICFKKKIVSHMDEYMFFPNQTRNMELLTEFKVDAELRKVVVESSLHHPTHLLPTG 60
           MFFPCICFKKKIVSHMDEYMFFPNQTRNMELLTEFKVDAELRKVVVESSLHHPTHLLPTG
Sbjct: 1   MFFPCICFKKKIVSHMDEYMFFPNQTRNMELLTEFKVDAELRKVVVESSLHHPTHLLPTG 60

Query: 61  TCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRT 120
           TCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRT
Sbjct: 61  TCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRT 120

Query: 121 TTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEP 180
           TTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEP
Sbjct: 121 TTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEP 180

Query: 181 TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREK 240
           TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREK
Sbjct: 181 TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREK 240

Query: 241 SKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAE 300
           SKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAE
Sbjct: 241 SKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAE 300

Query: 301 MWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISC 360
           MWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISC
Sbjct: 301 MWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISC 360

Query: 361 SFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKET 420
           SFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKET
Sbjct: 361 SFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKET 420

Query: 421 KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEI 480
           KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEI
Sbjct: 421 KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEI 480

Query: 481 QRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 524
           QRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN
Sbjct: 481 QRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 523

BLAST of CsaV3_7G026620 vs. NCBI nr
Match: XP_004149331.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 948.7 bits (2451), Expect = 2.1e-272
Identity = 480/481 (99.79%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 133 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 192

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 193 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 252

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV
Sbjct: 253 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 312

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS
Sbjct: 313 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 372

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 373 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 432

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL
Sbjct: 433 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 492

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 493 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 552

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS
Sbjct: 553 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 612

Query: 523 N 524
           N
Sbjct: 613 N 613

BLAST of CsaV3_7G026620 vs. NCBI nr
Match: XP_008458080.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 468/481 (97.30%), Postives = 471/481 (97.92%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 133 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 192

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 193 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 252

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV AV
Sbjct: 253 SEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAV 312

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHLETYACALGNVYS
Sbjct: 313 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYS 372

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 373 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 432

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKFGAALT DHLSYL
Sbjct: 433 QKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFGAALTADHLSYL 492

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 493 ADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 552

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 
Sbjct: 553 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVI 612

Query: 523 N 524
           N
Sbjct: 613 N 613

BLAST of CsaV3_7G026620 vs. NCBI nr
Match: TYJ96325.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 918.3 bits (2372), Expect = 3.0e-263
Identity = 467/481 (97.09%), Postives = 471/481 (97.92%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 137 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 196

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 197 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 256

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV AV
Sbjct: 257 SEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAV 316

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHLETYACALGNVYS
Sbjct: 317 QDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYS 376

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 377 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 436

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKFGAALT DHLSYL
Sbjct: 437 QKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFGAALTADHLSYL 496

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 497 ADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 556

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 
Sbjct: 557 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVI 616

Query: 523 N 524
           N
Sbjct: 617 N 617

BLAST of CsaV3_7G026620 vs. NCBI nr
Match: XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])

HSP 1 Score: 892.1 bits (2304), Expect = 2.3e-255
Identity = 448/481 (93.14%), Postives = 466/481 (96.88%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHL+PTGTCILVEG+LKQVPVPGKHVVRLEVEKILHVGKVEQ+KYPLSQ
Sbjct: 138 QVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEVEKILHVGKVEQQKYPLSQ 197

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASV RI +ALTFA HTFFQN+SFLYVQLPTITTTDSEGF
Sbjct: 198 KKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNHSFLYVQLPTITTTDSEGF 257

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGK+VEPTEDKE DG+SLETVKAAITEKSNLVKELERSESN+EALV AV
Sbjct: 258 SEKFHVTTLAGKGKKVEPTEDKEHDGVSLETVKAAITEKSNLVKELERSESNKEALVAAV 317

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTNELALQLEA+EKSK+KSRKDKVIPGNFFPQETYLT SGQLHLETYACALGNVYS
Sbjct: 318 QDLKKTNELALQLEAKEKSKTKSRKDKVIPGNFFPQETYLTASGQLHLETYACALGNVYS 377

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAEL++AMNCADDLVKFLSKWVL+HC+DDMKFV
Sbjct: 378 CGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADDLVKFLSKWVLDHCLDDMKFV 437

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLES+ISCSFEKVSY  AIEILEKAT+QKS HLKKLKFGAALT DHLSYL
Sbjct: 438 QKRIDKTSIHRLESVISCSFEKVSYIAAIEILEKATDQKSQHLKKLKFGAALTVDHLSYL 497

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           AD IFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER DVLMT
Sbjct: 498 ADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDVLMT 557

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS
Sbjct: 558 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 617

Query: 523 N 524
           N
Sbjct: 618 N 618

BLAST of CsaV3_7G026620 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 513.1 bits (1320), Expect = 3.8e-144
Identity = 277/492 (56.30%), Postives = 357/492 (72.56%), Query Frame = 0

Query: 43  KVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVEKILHVGKVEQEKYP 102
           +VVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE EK+LHVG V+ EKYP
Sbjct: 154 QVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAEKLLHVGTVDPEKYP 213

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           LS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + F YVQ+P ITTT  
Sbjct: 214 LSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHGFQYVQVPVITTT-- 273

Query: 163 EGFSEKFHVTTLAGKGKRVEPTEDKEP-----DGLSLETVKAAITEKSNLVKELERSESN 222
            GF E F VTTL GK    +  E+K+P     DG S++TVKA I EK+ L+  L+RS+SN
Sbjct: 274 TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKEKTRLIDHLKRSDSN 333

Query: 223 REALVTAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFFPQETYLTVSGQLHLE 282
           RE +V AV DLKKTN+LA Q+E ++KSK+ +  + +K+    +FF ++TYLT SG+ HLE
Sbjct: 334 RETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRDTYLTASGRFHLE 393

Query: 283 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 342
           +YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM+CAD+  KFL K+V
Sbjct: 394 SYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDCADEYFKFLCKYV 453

Query: 343 LEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFG 402
           LE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+KAT  K     K ++G
Sbjct: 454 LENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATTTKFE--TKPEWG 513

Query: 403 AALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGS 462
            ALT +HLSYL D I+K PVIV  YPK  K F VRLNDD KTVAAFD+V+PK GVVI+GS
Sbjct: 514 VALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFDLVVPKVGVVITGS 573

Query: 463 QKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDV 522
           Q EER ++L  R+ E G  RE++EWYL+++RHG VKHSG SL+ ++ +L+ TGL DIKD 
Sbjct: 574 QNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQMLLYATGLPDIKDA 633

Query: 523 IPFPRSFGKVSN 524
           IPFPRS+GK +N
Sbjct: 634 IPFPRSWGKANN 638

BLAST of CsaV3_7G026620 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.2e-118
Identity = 225/486 (46.30%), Postives = 322/486 (66.26%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQEKYP 102
           +V+V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  KYP
Sbjct: 85  QVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYP 144

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           L + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT+D 
Sbjct: 145 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 204

Query: 163 EGFSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREA 222
           EG  E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ +++++EA
Sbjct: 205 EGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEA 264

Query: 223 LVTAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFFPQETYLTVSGQLHLETYA 282
           +  AV +LK   E    ++ R + +    +KD  I    +FF ++ +LTVSGQL +ETYA
Sbjct: 265 ITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYA 324

Query: 283 CALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEH 342
           CAL NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+LE 
Sbjct: 325 CALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEK 384

Query: 343 CMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAAL 402
           C  DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +       +++G  L
Sbjct: 385 CYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDL 444

Query: 403 TDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKE 462
             +H  YL + +F+KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+E
Sbjct: 445 ASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQRE 504

Query: 463 ERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPF 521
           ER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R +LF TGL +I+DVIPF
Sbjct: 505 ERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPF 564

BLAST of CsaV3_7G026620 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.2e-113
Identity = 223/488 (45.70%), Postives = 317/488 (64.96%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQEKYP 102
           +V+V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+   YP
Sbjct: 82  QVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYP 141

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           L + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT+D 
Sbjct: 142 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 201

Query: 163 EGFSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREA 222
           EG  E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ +++++E 
Sbjct: 202 EGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEE 261

Query: 223 LVTAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FFPQETYLTVSGQLHLETYA 282
           +  +V  L         +E R + K    K+  K+   N FF ++ +LTVSGQL +ETYA
Sbjct: 262 ITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYA 321

Query: 283 CALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEH 342
           CAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+++ 
Sbjct: 322 CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDK 381

Query: 343 CMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQ-KSPHLKKLKFGAA 402
           C DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++G  
Sbjct: 382 CGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGID 441

Query: 403 LTDDHLSYLADTIF-KKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQ 462
           L  +H  YL +  F +KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ
Sbjct: 442 LASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 501

Query: 463 KEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVI 521
           +EER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R + F TG+ +I+DVI
Sbjct: 502 REERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVI 561

BLAST of CsaV3_7G026620 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.5e-92
Identity = 191/465 (41.08%), Postives = 264/465 (56.77%), Query Frame = 0

Query: 57  LPTGTCILVEGVLKQVPVPGKHV-VRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSY 116
           L TG  +   G +  V  PGK   + ++   I  +GK + E Y L +K+   +FLR  ++
Sbjct: 70  LSTGVSVSAIGTI--VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFEFLRSIAH 129

Query: 117 FRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKG 176
            RPRT T+ +VTR+R+AL FA H FFQ   FLY+  P IT +D EG  + F VTTL    
Sbjct: 130 LRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQVTTLDQNN 189

Query: 177 KRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQL 236
               P                                   E  V   QD           
Sbjct: 190 PARTP-----------------------------------EGRVDYTQD----------- 249

Query: 237 EAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESA 296
                              FF + TYLTVSGQL+ E YACAL +VY+ GP FRA+   ++
Sbjct: 250 -------------------FFGKPTYLTVSGQLNGEIYACALSDVYTFGPTFRAENSNTS 309

Query: 297 KHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLE 356
           +H AE WM+E E+AFA+L D M+CA+D +K++ K+VL++C +DM+F  K +  + I RLE
Sbjct: 310 RHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFNKHVATDLISRLE 369

Query: 357 SIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCN 416
            +I+ SFE+ SYT A+ ILEKA  +       +K+G  L  +H  +LA+  F KPVI+ +
Sbjct: 370 HVINTSFERASYTYAVRILEKADKKFE---YPVKWGLDLQSEHERFLAEEFFGKPVILTD 429

Query: 417 YPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYE 476
           YPK+ K F +R N+D KTVAA DV++PK G +I GSQ+EERL VL ++++E  LP E+Y 
Sbjct: 430 YPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYW 464

Query: 477 WYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 521
           WYLE+++ G V HSGF   F+R V FTTG+ +I+DVIPFPR  GK
Sbjct: 490 WYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGK 464

BLAST of CsaV3_7G026620 vs. ExPASy Swiss-Prot
Match: Q73P19 (Asparagine--tRNA ligase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=asnS PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 4.3e-87
Identity = 185/463 (39.96%), Postives = 260/463 (56.16%), Query Frame = 0

Query: 59  TGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRP 118
           TG  + V G L   P  G+  V L+   I   G  +QEKYPL +K+  M+FLR  ++ R 
Sbjct: 70  TGVSVKVSGNLVPSPAAGQR-VELQANNIHIFGDADQEKYPLQKKRHSMEFLRDIAHLRA 129

Query: 119 RTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRV 178
           RT T  +V RIRS + +A HTFFQ   F YV  P IT +D EG  E FHVTT        
Sbjct: 130 RTNTFGAVARIRSQMAYAIHTFFQERGFQYVHTPIITGSDCEGAGEMFHVTTF------- 189

Query: 179 EPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAR 238
               D E      ETVK A+ EK +                                   
Sbjct: 190 ----DIE------ETVKKALKEKKD----------------------------------P 249

Query: 239 EKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHA 298
           +  K    +D      FF ++  LTVSGQL  ETYA AL  +Y+ GP FRA+   +++H 
Sbjct: 250 DSFKIDYSQD------FFGKQANLTVSGQLEGETYATALSRIYTFGPTFRAENSNTSRHL 309

Query: 299 AEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESII 358
           AE WMVE E++F  +K+ M  A++ + +L KW LE C +D++F   RI K  I  L++++
Sbjct: 310 AEFWMVEPEMSFFTIKENMELAEEFIVYLLKWALEKCKEDLEFFDSRIKKGLIEMLKNVV 369

Query: 359 SCSFEKVSYTTAIEILEKATNQKSPHLKKLKF----GAALTDDHLSYLADTIFKKPVIVC 418
           +  F +++YT AI  LEK       H+ + +F    G  L  +H  +L + ++K PVIV 
Sbjct: 370 NTPFTRLTYTEAIAELEK-------HIDRFEFKPYWGCDLQSEHERFLTEEVYKGPVIVT 429

Query: 419 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQY 478
           NYPKE K F ++LN+DGKTV A DV++P  G +I GS++EE LD+L  R++E+GL  E Y
Sbjct: 430 NYPKEIKSFYMKLNEDGKTVRAMDVLVPGLGEIIGGSEREENLDILQGRIKELGLREEDY 467

Query: 479 EWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS 518
            WYL+++R+G V HSGF L F+R +L+ TG+ +I+DVIPFPR+
Sbjct: 490 WWYLDLRRYGTVPHSGFGLGFERLLLYVTGMGNIRDVIPFPRA 467

BLAST of CsaV3_7G026620 vs. ExPASy TrEMBL
Match: A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 1.0e-272
Identity = 480/481 (99.79%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 133 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 192

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 193 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 252

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV
Sbjct: 253 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 312

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS
Sbjct: 313 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 372

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 373 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 432

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL
Sbjct: 433 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 492

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 493 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 552

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS
Sbjct: 553 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 612

Query: 523 N 524
           N
Sbjct: 613 N 613

BLAST of CsaV3_7G026620 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.5e-264
Identity = 468/481 (97.30%), Postives = 471/481 (97.92%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 133 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 192

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 193 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 252

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV AV
Sbjct: 253 SEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAV 312

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHLETYACALGNVYS
Sbjct: 313 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYS 372

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 373 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 432

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKFGAALT DHLSYL
Sbjct: 433 QKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFGAALTADHLSYL 492

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 493 ADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 552

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 
Sbjct: 553 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVI 612

Query: 523 N 524
           N
Sbjct: 613 N 613

BLAST of CsaV3_7G026620 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.4e-263
Identity = 467/481 (97.09%), Postives = 471/481 (97.92%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 137 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 196

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 197 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 256

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV AV
Sbjct: 257 SEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAV 316

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHLETYACALGNVYS
Sbjct: 317 QDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYS 376

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 377 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 436

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKFGAALT DHLSYL
Sbjct: 437 QKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFGAALTADHLSYL 496

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 497 ADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 556

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 
Sbjct: 557 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVI 616

Query: 523 N 524
           N
Sbjct: 617 N 617

BLAST of CsaV3_7G026620 vs. ExPASy TrEMBL
Match: A0A5A7SMX6 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00190 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.4e-250
Identity = 451/481 (93.76%), Postives = 457/481 (95.01%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ
Sbjct: 137 QVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 196

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF
Sbjct: 197 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 256

Query: 163 SEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAV 222
           SEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV AV
Sbjct: 257 SEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAV 316

Query: 223 QDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYS 282
           QDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHLETYACALGNVYS
Sbjct: 317 QDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYS 376

Query: 283 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 342
           CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV
Sbjct: 377 CGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFV 436

Query: 343 QKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYL 402
           QKRIDK SIHRLESIISCSFEKVSYT AIEILEK  ++ S  L    F    T +  SYL
Sbjct: 437 QKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLSTLLSCDSFFLLCTLN--SYL 496

Query: 403 ADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 462
           ADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT
Sbjct: 497 ADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMT 556

Query: 463 RMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVS 522
           RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 
Sbjct: 557 RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVI 615

Query: 523 N 524
           N
Sbjct: 617 N 615

BLAST of CsaV3_7G026620 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 7.5e-236
Identity = 418/484 (86.36%), Postives = 446/484 (92.15%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQ 102
           +VVVESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLEVEKILHVGKVEQEKYPL+Q
Sbjct: 139 QVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKILHVGKVEQEKYPLTQ 198

Query: 103 KKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGF 162
           KKLPMD LRKFSYFRPRTTTVASV RI +ALTFA HTFFQN+ FLYVQLPTITTTDSEGF
Sbjct: 199 KKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNHGFLYVQLPTITTTDSEGF 258

Query: 163 SEKFHVTTLAGKG--KRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVT 222
           SEKFHVTT AGK   K++EPTEDKE DG+SLETVKAA  EK N+VKELERSESNREAL  
Sbjct: 259 SEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKRNIVKELERSESNREALAA 318

Query: 223 AVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQETYLTVSGQLHLETYACALGN 282
           AVQDLKKTNELALQLEAR K KS+ +KDKV I  NFFPQETYLTVSGQLHLETYACALGN
Sbjct: 319 AVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETYLTVSGQLHLETYACALGN 378

Query: 283 VYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDM 342
           VYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAEL+D +NCADDLVKFLSKWVLEHC+DDM
Sbjct: 379 VYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVLEHCLDDM 438

Query: 343 KFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHL 402
           KFVQKRIDK S+HRLES I+ SFEKVSYT A+EIL+KAT+QKS  LK+LKFGAALTDDHL
Sbjct: 439 KFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKATDQKSQRLKRLKFGAALTDDHL 498

Query: 403 SYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDV 462
           SYLAD IFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER DV
Sbjct: 499 SYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDV 558

Query: 463 LMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFG 522
           L++RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS G
Sbjct: 559 LLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSLG 618

Query: 523 KVSN 524
           KVS+
Sbjct: 619 KVSH 622

BLAST of CsaV3_7G026620 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.7e-145
Identity = 277/492 (56.30%), Postives = 357/492 (72.56%), Query Frame = 0

Query: 43  KVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVEKILHVGKVEQEKYP 102
           +VVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE EK+LHVG V+ EKYP
Sbjct: 154 QVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAEKLLHVGTVDPEKYP 213

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           LS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + F YVQ+P ITTT  
Sbjct: 214 LSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHGFQYVQVPVITTT-- 273

Query: 163 EGFSEKFHVTTLAGKGKRVEPTEDKEP-----DGLSLETVKAAITEKSNLVKELERSESN 222
            GF E F VTTL GK    +  E+K+P     DG S++TVKA I EK+ L+  L+RS+SN
Sbjct: 274 TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKEKTRLIDHLKRSDSN 333

Query: 223 REALVTAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFFPQETYLTVSGQLHLE 282
           RE +V AV DLKKTN+LA Q+E ++KSK+ +  + +K+    +FF ++TYLT SG+ HLE
Sbjct: 334 RETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRDTYLTASGRFHLE 393

Query: 283 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 342
           +YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM+CAD+  KFL K+V
Sbjct: 394 SYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDCADEYFKFLCKYV 453

Query: 343 LEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFG 402
           LE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+KAT  K     K ++G
Sbjct: 454 LENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATTTKFE--TKPEWG 513

Query: 403 AALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGS 462
            ALT +HLSYL D I+K PVIV  YPK  K F VRLNDD KTVAAFD+V+PK GVVI+GS
Sbjct: 514 VALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFDLVVPKVGVVITGS 573

Query: 463 QKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDV 522
           Q EER ++L  R+ E G  RE++EWYL+++RHG VKHSG SL+ ++ +L+ TGL DIKD 
Sbjct: 574 QNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQMLLYATGLPDIKDA 633

Query: 523 IPFPRSFGKVSN 524
           IPFPRS+GK +N
Sbjct: 634 IPFPRSWGKANN 638

BLAST of CsaV3_7G026620 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 8.7e-120
Identity = 225/486 (46.30%), Postives = 322/486 (66.26%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQEKYP 102
           +V+V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  KYP
Sbjct: 85  QVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYP 144

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           L + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT+D 
Sbjct: 145 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 204

Query: 163 EGFSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREA 222
           EG  E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ +++++EA
Sbjct: 205 EGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEA 264

Query: 223 LVTAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFFPQETYLTVSGQLHLETYA 282
           +  AV +LK   E    ++ R + +    +KD  I    +FF ++ +LTVSGQL +ETYA
Sbjct: 265 ITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYA 324

Query: 283 CALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEH 342
           CAL NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+LE 
Sbjct: 325 CALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEK 384

Query: 343 CMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAAL 402
           C  DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +       +++G  L
Sbjct: 385 CYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDL 444

Query: 403 TDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKE 462
             +H  YL + +F+KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+E
Sbjct: 445 ASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQRE 504

Query: 463 ERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPF 521
           ER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R +LF TGL +I+DVIPF
Sbjct: 505 ERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPF 564

BLAST of CsaV3_7G026620 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 8.4e-115
Identity = 223/488 (45.70%), Postives = 317/488 (64.96%), Query Frame = 0

Query: 43  KVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQEKYP 102
           +V+V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+   YP
Sbjct: 82  QVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYP 141

Query: 103 LSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDS 162
           L + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT+D 
Sbjct: 142 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 201

Query: 163 EGFSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREA 222
           EG  E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ +++++E 
Sbjct: 202 EGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEE 261

Query: 223 LVTAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FFPQETYLTVSGQLHLETYA 282
           +  +V  L         +E R + K    K+  K+   N FF ++ +LTVSGQL +ETYA
Sbjct: 262 ITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYA 321

Query: 283 CALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEH 342
           CAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+++ 
Sbjct: 322 CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDK 381

Query: 343 CMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQ-KSPHLKKLKFGAA 402
           C DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++G  
Sbjct: 382 CGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGID 441

Query: 403 LTDDHLSYLADTIF-KKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQ 462
           L  +H  YL +  F +KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ
Sbjct: 442 LASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 501

Query: 463 KEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVI 521
           +EER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R + F TG+ +I+DVI
Sbjct: 502 REERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVI 561

BLAST of CsaV3_7G026620 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-83
Identity = 182/461 (39.48%), Postives = 266/461 (57.70%), Query Frame = 0

Query: 59  TGTCILVEGVLKQVPVPG-KHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFR 118
           TG  + V+G +  V   G K  V L+VEKI+ VG+ +   YP+ +K++  +FLR  ++ R
Sbjct: 162 TGASVSVQGTI--VASQGTKQKVELKVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLR 221

Query: 119 PRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKR 178
           PRT T  +V R+R+ L +A H FFQ + F++V  P IT +D EG  E+F VTTL      
Sbjct: 222 PRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLI----- 281

Query: 179 VEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEA 238
                                              S+ E   T++  + KT    +    
Sbjct: 282 ----------------------------------PSSHENTDTSIDAIPKTKGGLIDWSQ 341

Query: 239 REKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKH 298
                           +FF +  +LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H
Sbjct: 342 ----------------DFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRH 401

Query: 299 AAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESI 358
            AE WM+E E+AFA+L D M CA   ++++ K+VL++C +DM+F    I+K  I RL  +
Sbjct: 402 LAEFWMIEPELAFADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDV 461

Query: 359 ISCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIF-KKPVIVCNY 418
               F ++ YT AIEIL KA N+K      +K+G  L  +H  Y+ +  F  +PVI+ +Y
Sbjct: 462 AEKEFLQLGYTDAIEILLKA-NKKFDF--PVKWGLDLQSEHERYITEEAFGGRPVIIRDY 521

Query: 419 PKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEW 478
           PKE K F +R NDDGKTVAA D+++P+ G +I GSQ+EERL+VL  R+ E+ L +E Y W
Sbjct: 522 PKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWW 561

Query: 479 YLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS 518
           YL+++R+G V H+GF L F+R V F TG+ +I+DVIPFPR+
Sbjct: 582 YLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPRT 561

BLAST of CsaV3_7G026620 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-15
Identity = 35/77 (45.45%), Postives = 58/77 (75.32%), Query Frame = 0

Query: 277 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 336
           +G+VY+  P FRA+K  +++H AE WMVEVE+AFA +++AMNC++ +VK +   +LE C 
Sbjct: 1   MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60

Query: 337 DDMKFVQKRIDKNSIHR 354
           DDM+++ +++D+  I R
Sbjct: 61  DDMEYMVEKVDEFCIDR 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031745191.15.8e-299100.00asparagine--tRNA ligase, cytoplasmic 2 isoform X2 [Cucumis sativus] >KAE8646266.... [more]
XP_004149331.12.1e-27299.79asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus][more]
XP_008458080.11.3e-26397.30PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo][more]
TYJ96325.13.0e-26397.09asparagine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_038899280.12.3e-25593.14asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SW953.8e-14456.30Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ... [more]
Q9SW961.2e-11846.30Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK11.2e-11345.70Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC91.5e-9241.08Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
Q73P194.3e-8739.96Asparagine--tRNA ligase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / ... [more]
Match NameE-valueIdentityDescription
A0A0A0K8G11.0e-27299.79Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1[more]
A0A1S3C6Z56.5e-26497.30Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1[more]
A0A5D3BD071.4e-26397.09Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7SMX62.4e-25093.76Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1KTV97.5e-23686.36asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G07420.12.7e-14556.30asparaginyl-tRNA synthetase 2 [more]
AT5G56680.18.7e-12046.30Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.18.4e-11545.70Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.12.2e-8339.48Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G68420.11.5e-1545.45Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 191..228
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 99..522
e-value: 4.8E-85
score: 287.9
NoneNo IPR availablePANTHERPTHR22594:SF36ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATEDcoord: 43..523
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 43..523
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 107..517
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 478..492
score: 32.58
coord: 262..274
score: 41.78
coord: 438..454
score: 27.94
coord: 278..291
score: 32.47
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 108..170
e-value: 2.0E-7
score: 30.4
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 248..516
e-value: 4.4E-52
score: 177.1
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 44..521
e-value: 8.7E-120
score: 398.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G026620.1CsaV3_7G026620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding