CsaV3_7G008200 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_7G008200
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionLipase
Locationchr7: 5135218 .. 5140152 (-)
RNA-Seq ExpressionCsaV3_7G008200
SyntenyCsaV3_7G008200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGCCATTGCTTGCTTTCATATTTCTTCTCTTCTTCTTCCCTCTCCATTCCGCCGGTGATTCCTCCTTCCCTTCTGTCGTTGACTACTCGATTCTCCCTCACGCTTTGTCCGACAACAAGTCCTTGTGCTCCCAACTCGTTCTTCCTGCTGGCTATCCTTGTGCTGAGCATAAGGTTCTCTATTTCTCTCATTTCGCTTCCAATTTTTTGGTTGATTAATTAGAGGAAGGTGTGGACAATTATGTGAAATGAGACTGTAATATTAGAGTTCATCGCTCTGTTTCGACTGGAGATACTTGGATGGATTTTAGTCAACTAATTGCCTCTAATGCTTCTTGTTTGAGATTTCCTTTGAGTTTCTTGTTTATTTTCTTTTCTGAGGTGAGACAGATGGAATTAATTTCTTTGTATTTTTAGATTCGATGCCCGACGTGGTTTTGATTTGTTTCTGTCGATTTTCTGGATAATCCGATGTATATGTATCGTTCTCGAAAGTGTGATATGCGAAGAAATATGATTGAATTTGATTTTTATGTAGATCTCTGATTTTATCAATTTCTCATTAACCATTTGGAGCTTAATGCATCTCTACTGTGTAAATTTGTTACTATAAATGATGTTGTTCTTTGATCATGCTCGAGTTTTGAGGAATGTTATCCATTATTGCAGACTGGGCTGATGAATTGTGCTTTTAGTAATGCACTTGAGTACGGTTTTGCAGTACTGAGTCACTGACGGACGTGATTTTCTCATAAATTTCTTAGATTCAAACAAAGGATGGGTTTTTATTGGGTCTGCAACGTGTATCTTCACGTGATGGAGAATTGGAAAAGCAAAAAGGTCCTCCAATTTTGCTTCTCCATGGATTGTTTATGGTAAGTGTTCTGACAGTTTTGCAATATTGTAACTGTATCTGTTAGGTCTTTTTACTCTTTTTTTGGTCTAACTCTATTAGCAATAGCTAGGGTATTGACATTGCTGCCTGATATCTTTTATACTACCGAGACTTATCTCCTTTTGTTGTAATTTTCAACTGCTCCCATCTCTTCATATTCCAGTGGTATTTAATGTTCTCGATTAGGTCGATATTTACCTTAAACTTCTACACTACAGTCCAATAGTGTAACCATGCTTTCCAGTTATCTCTCAAGGAATTGGTACTAGAAGATGAATGGCATATAGCCTCAAGGCAGATTTTACCCAAGGCCCTATCTTTTATATATATATGTCTCTCTCTTTTTAATCTTCTGGCCTCTGTTTTCTTGTCTCTCCAAGTGAGATTCTTTTCAAATTCTTTTTATTCTCATCCCAAAGTGTACTGAATTGTCTTCTTTAGTTTCATTCGTTGGTACAACATTTCTATGCTTGGGAAGATCCAATTATCCATAAATTTCTTTTTGTCTAGGAGGTTAGTAACTGCGTATTTTGAAACATGGATGGTGAATTTTGAATAAGTTTGTAGTCTGGGATCATTCTCACAGTAAGGGGTTCCAACCACTAGTTTTTCGTACCTTTCATTGTCTGAGCTGGTGCATGTGCAGGCTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTCCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTTATGGGCATTCATCCCTATCAGAGGATGAGAAGGTATTCTTAGTGTACTTTATCACAAAATTTTAAGATGTGTAAGATGTTAATTAAATGCAGAGAAAGAACATAGTTTCCCCTATTGTGTTGTGGATGTATTGCATGTATCTAGGAAGTTAAATAATGATACAAACATGGTGTAGCTCATGCAGGCAATTTTGACGCTTCAAACTTCAAACAAATTTACATGACGTTTTATGGAGTTAAGCAGTCGCATGACAGATTATCTTCAAGGATCATTTGATTTCATGCTAATTGTGTTGATTTGCTAAAGTTTATTTTATCTCAGAATTCTGCACTTTATTGTATGGTGGATTCTTGGTCCACAAGTATGATAGGACAATCTTGAAAGTACTCTAACATAAATTTCATTTGTTTAGTTCTATTTATGTCTTTGACTCTAGTATATTATGATTGTAGGAATTTTGGAATTGGAGTTGGGAAGAGTTGGCCCTGTACGATCTTGCAGAAATGATTAACTACATAAATTCCTTGACAAACAAGAAAATTTATATTGTGGGACACTCACAGGTCTCATATCTCTTCTTCTACGAGATTGTTGCTCTGAAATGATATATTTCTGGTATGGTGTCATCTTTTATCTTTGTTCGCAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGGAGCAGCTGCTCTTTTAAGTCCTATATCGTACTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGGTATTCTTGGCTTGTTATTGTGCAATTAGCATTGTTAAGTGATTGTATTATTAATAGCAATAAGCTTATTTTTTATATTTTTTTCGACAGATAATTCTTGCCTCAGGATTTCATGAACTCAACTTTAAAAGGTTGTCATGCTATGCTGTGCCCATCTCTAACTTTGTAATGGATTGCATTTTTAGCTTCACCATAGTTCATGGTACTCATGCCTATGTTTTTGTATGCTATTTCAGTGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTAGTTAACTGCATCAATATTCTAAGTTCAATAACAGGTTCTCTCAATGCAACCTGCTTCACGTTTCTCTCTTTTCTTTTTATTTATTATTTTTGTGAAACCTTGACTTTGGAATCCTTATCCAACATACTTGGATGATCCTTTCTTTAACCGAATACGAATCTTTCGATTCATTTGGATCTCCTCTCACGCTTATTTATTGCTTTTTACTTTTGGGAAATAATTGAACACTAATCCAAGACTCTAATATTCAGAGGGCTCTATAATTGTCAGCAAAGTTGTTTTCCTTTTCTTGAAATCCAAAAACAAAAATTCTTACTTTATACATAACATAGGTCATCCATTCAACATGGGATAAAAAAGAAACAAATGAGGGTGAAATCTTGCTAATTTCTTTTAATTACTATATCTTATACTGACATCAATCATATTGGTTCATAATTTTGTGTGGTTAGTGGGTATCTCTCTTAAAACTGAAGGGCTTGGGTTGGAAAATATGACTAAAATTTACCTCGTGATGGAATTGGGAAAAATATTACTGATATTTACCAAGTTCCCCATTTTTTACATGATATTGCTGCCAGCAAGCTTCTATTAGACATATTGCAATTCAAGAAATGGACTACCAGCTTCCATTTTTTGCTCTGATGCATATCTGCATTTTCATCCATTTTATTGAACCTTAACTTCATTTGCTATTATGAAGTTTGGATGTTCAACACTAAATTTCCTAAGGATGCCTTCTTTTAAATGCTGCAGGCGAAAATTGCTGCCTGAATCGCTCACGTTTTGACTTATTTTTCAAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGAGTATGCTCTGCAATATCTTCTCTCTACTTCAACTTCTATCATACTATGACTATGATTTCGATGAATCTTTGAATGTTTTACCTGCATGTATTTCTTGGAAAGTTAATCATAGTGTTCTCTTGTGAAGTCTAATATAAATCTTTAAACATTTGATCGTGAAGCTTTGCATCATACATTCTAAACTCTTTCAAGACTTAGAAGATAATTTGATCTTCCTCACATGACATTTTTCTAGAAACCTTTTAAAGATATCAAAATTAACCCAACCTCAACCTAAAGGATACTCAGCTTTCCACAAACAGATTTTCATTCAGACACCAAATTTGCTAATACTCTGCAACCTTAAGCAGTTTTTCTTCCTTTGGCTTCCTTCTTTTAATTTGCTTCCCACTTTCCCGATTTTTCAAATAATCGAGGAGGCTATAAAGGATTTTTTTTTCATATGCTCATTAACTTCTTTCGCTCAATCTCTTTCCTGAGTGACTTTTCATCATCCCTCAAATCCCTCGCTTTCCATTAACTTTATCTATAATTAAAGCCAGCCCTCTATTTCCATAGTATTTTTATCAATTACCGAACTCGTTTCCTCTTTTCACTCCCTTGTCCTTACCTTCGCTTTCCACCTATTCCTTTCAATCAAAGCTTCAATTTCCGCTTGCGCCACCAGAACTTTTCTGTTCTCTTTAACATTAGGCACAAACAACATAATCGAACTTTGCTTATTATTACCAAGCCGCTTGCTCTTTAGCAGTTCATTTTTCTCCATCTTGTACTTCCAAGTCCGAACATTAGTGATGGTTTAGGATCAATTTTTCTGTTGTTAGTTCTATTAATTTTATTAGGAGAGTGTATACTCGACTTCAACACATGGCAACAATTTACTACACTGAAGTTGAATTATTGGTTTTAGGAAGTGTTATAGTCTACAAATCAGTTGCTTGGTTAAGACTTAAGCAGTATGTTTTCTCCTCCTGACGGGGATACGACGTGGTTCGACTCCACGGACTTAACTTCCTTTTCCTTTTCTTCCACCAGTGATCCGCAAAGGTAGTTTTTCCAAGTATGACTATGGATTGTTAAAGAATCTGAGAGTTTATGGTCAAAGGGTGCCACCTGAATTTGATCTCAGCCGCATTCCTGAATCCTTGCCGCTGTGGATGGCATACGGCGGGAACGATGAGTTGTCAGATTGGACAGATCTGGAGAACACTATAAAGAAGGTGAAGTCGGTACCAGAATTAGTTTACCTTGAGAATTATGGTCATGTGGATTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAGTTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

mRNA sequence

ATGGCGATGCCATTGCTTGCTTTCATATTTCTTCTCTTCTTCTTCCCTCTCCATTCCGCCGGTGATTCCTCCTTCCCTTCTGTCGTTGACTACTCGATTCTCCCTCACGCTTTGTCCGACAACAAGTCCTTGTGCTCCCAACTCGTTCTTCCTGCTGGCTATCCTTGTGCTGAGCATAAGATTCAAACAAAGGATGGGTTTTTATTGGGTCTGCAACGTGTATCTTCACGTGATGGAGAATTGGAAAAGCAAAAAGGTCCTCCAATTTTGCTTCTCCATGGATTGTTTATGGCTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTCCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTTATGGGCATTCATCCCTATCAGAGGATGAGAAGGAATTTTGGAATTGGAGTTGGGAAGAGTTGGCCCTGTACGATCTTGCAGAAATGATTAACTACATAAATTCCTTGACAAACAAGAAAATTTATATTGTGGGACACTCACAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGGAGCAGCTGCTCTTTTAAGTCCTATATCGTACTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGATAATTCTTGCCTCAGGATTTCATGAACTCAACTTTAAAAGGTTGTCATGCTATGCTGTGCCCATCTCTAACTTTGTAATGGATTGCATTTTTAGCTTCACCATAGTTCATGGTACTCATGCCTATGTTTTTGTATGCTATTTCAGTGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTAGTTAACTGCATCAATATTCTAAGTTCAATAACAGGCGAAAATTGCTGCCTGAATCGCTCACGTTTTGACTTATTTTTCAAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGATGATCCGCAAAGGTAGTTTTTCCAAGTATGACTATGGATTGTTAAAGAATCTGAGAGTTTATGGTCAAAGGGTGCCACCTGAATTTGATCTCAGCCGCATTCCTGAATCCTTGCCGCTGTGGATGGCATACGGCGGGAACGATGAGTTGTCAGATTGGACAGATCTGGAGAACACTATAAAGAAGGTGAAGTCGGTACCAGAATTAGTTTACCTTGAGAATTATGGTCATGTGGATTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAGTTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Coding sequence (CDS)

ATGGCGATGCCATTGCTTGCTTTCATATTTCTTCTCTTCTTCTTCCCTCTCCATTCCGCCGGTGATTCCTCCTTCCCTTCTGTCGTTGACTACTCGATTCTCCCTCACGCTTTGTCCGACAACAAGTCCTTGTGCTCCCAACTCGTTCTTCCTGCTGGCTATCCTTGTGCTGAGCATAAGATTCAAACAAAGGATGGGTTTTTATTGGGTCTGCAACGTGTATCTTCACGTGATGGAGAATTGGAAAAGCAAAAAGGTCCTCCAATTTTGCTTCTCCATGGATTGTTTATGGCTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTCCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTTATGGGCATTCATCCCTATCAGAGGATGAGAAGGAATTTTGGAATTGGAGTTGGGAAGAGTTGGCCCTGTACGATCTTGCAGAAATGATTAACTACATAAATTCCTTGACAAACAAGAAAATTTATATTGTGGGACACTCACAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGGAGCAGCTGCTCTTTTAAGTCCTATATCGTACTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGATAATTCTTGCCTCAGGATTTCATGAACTCAACTTTAAAAGGTTGTCATGCTATGCTGTGCCCATCTCTAACTTTGTAATGGATTGCATTTTTAGCTTCACCATAGTTCATGGTACTCATGCCTATGTTTTTGTATGCTATTTCAGTGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTAGTTAACTGCATCAATATTCTAAGTTCAATAACAGGCGAAAATTGCTGCCTGAATCGCTCACGTTTTGACTTATTTTTCAAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGATGATCCGCAAAGGTAGTTTTTCCAAGTATGACTATGGATTGTTAAAGAATCTGAGAGTTTATGGTCAAAGGGTGCCACCTGAATTTGATCTCAGCCGCATTCCTGAATCCTTGCCGCTGTGGATGGCATACGGCGGGAACGATGAGTTGTCAGATTGGACAGATCTGGAGAACACTATAAAGAAGGTGAAGTCGGTACCAGAATTAGTTTACCTTGAGAATTATGGTCATGTGGATTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAGTTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Protein sequence

MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL*
Homology
BLAST of CsaV3_7G008200 vs. NCBI nr
Match: KAE8645852.1 (hypothetical protein Csa_017220 [Cucumis sativus])

HSP 1 Score: 909.8 bits (2350), Expect = 9.0e-261
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS
Sbjct: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV
Sbjct: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 442

BLAST of CsaV3_7G008200 vs. NCBI nr
Match: XP_004136959.1 (triacylglycerol lipase 1 [Cucumis sativus])

HSP 1 Score: 819.3 bits (2115), Expect = 1.6e-233
Identity = 407/442 (92.08%), Postives = 407/442 (92.08%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCINILS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINILS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV
Sbjct: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 407

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 407

BLAST of CsaV3_7G008200 vs. NCBI nr
Match: XP_008454984.1 (PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa] >TYK06787.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 785.8 bits (2028), Expect = 2.0e-223
Identity = 386/442 (87.33%), Postives = 401/442 (90.72%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFF+YEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQR+
Sbjct: 301 SITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRM 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+TIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMK 407

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 407

BLAST of CsaV3_7G008200 vs. NCBI nr
Match: XP_038886839.1 (triacylglycerol lipase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 737.3 bits (1902), Expect = 8.0e-209
Identity = 362/442 (81.90%), Postives = 389/442 (88.01%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL++ IFLLFFFPLHSA +SSF S V+YSILPHA SD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MATPLMSLIFLLFFFPLHSAHNSSFSS-VEYSILPHARSDQKSLCSQLVHPAGYPCAEHT 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDG+LEK+KGPPILLLHGLFM GDGWFLNSA+QSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGDLEKRKGPPILLLHGLFMGGDGWFLNSAKQSLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSED+KEFWNWSWEELALYDLA MINYINSLT++KIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDDKEFWNWSWEELALYDLAAMINYINSLTDRKIYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKV AAALLSPISYLEHITAPLVRLMVDTHLDTIIL++GF ELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILSAGFRELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFF+YEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKN+RVYGQR 
Sbjct: 301 SITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNMRVYGQRK 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLSRIP+SLPLWMAYGGNDELSDWTDLE+TIK++K VPELVYLENYGHVDFILSM 
Sbjct: 361 PPEFDLSRIPKSLPLWMAYGGNDELSDWTDLEHTIKELKPVPELVYLENYGHVDFILSMN 406

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AK+D+YDPMIKFFKSL KSSS+
Sbjct: 421 AKKDLYDPMIKFFKSLAKSSSM 406

BLAST of CsaV3_7G008200 vs. NCBI nr
Match: XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 722.2 bits (1863), Expect = 2.7e-204
Identity = 356/439 (81.09%), Postives = 383/439 (87.24%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S ++YSILP ALSD KSLCSQLV P GYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRS-LEYSILPRALSDQKSLCSQLVQPVGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+S+SEDEKEFW+WSWE+LALYDLA MI+YINSLT +KIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLD+LCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDDLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLN SRFDLFF+YEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR 
Sbjct: 301 SITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRK 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TIK++KSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMK 403

Query: 421 AKEDVYDPMIKFFKSLGKS 440
           AKEDVYDPMIKF KSLGKS
Sbjct: 421 AKEDVYDPMIKFLKSLGKS 403

BLAST of CsaV3_7G008200 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 7.9e-135
Identity = 234/415 (56.39%), Postives = 296/415 (71.33%), Query Frame = 0

Query: 27  SVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKG 86
           S +  S L H  S   SLC+ L+ PA Y C EH IQTKDG++L LQRV+S    L  Q G
Sbjct: 16  SALTQSHLLHG-SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSG 75

Query: 87  PPILLLHGLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEF 146
           PP+LL HGLFMAGD WFLNS ++SLGFIL D+GFDVW+GNVRGTR+SYGH +LS+ +KEF
Sbjct: 76  PPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEF 135

Query: 147 WNWSWEELALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLS 206
           W+WSW++LA+YDLAEMI Y+ S++N KI++VGHSQGTIMSFAALTQP +A+ V AAALL 
Sbjct: 136 WDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLC 195

Query: 207 PISYLEHITAPLVRLMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVH 266
           PISYL+H+TAPLV  MV  HLD +++A G H++NF+                        
Sbjct: 196 PISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFR------------------------ 255

Query: 267 GTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSS 326
                      SD    L+D+LC+  ++C + L+SITG NCC N S+ + +  YEPHPSS
Sbjct: 256 -----------SDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSS 315

Query: 327 AKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELS 386
            KN+ HLFQMIRKG+F++YDYG  KNLR YG   PPEF LS IP SLP+WM YGG D L+
Sbjct: 316 VKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLA 375

Query: 387 DWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSS 442
           D TD+E+T+ ++ S PEL+YLE+YGH+DF+L   AKEDVY  MI+FF++  KSSS
Sbjct: 376 DVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFFRAKVKSSS 392

BLAST of CsaV3_7G008200 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-74
Identity = 148/399 (37.09%), Postives = 226/399 (56.64%), Query Frame = 0

Query: 44  LCSQLVLPAGYPCAEHKIQTKDGFLLGLQRV-SSRDGEL--EKQKGPPILLLHGLFMAGD 103
           +C+  V   GY C EH + T+DG++L +QR+   R G +  +  K  P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 104 GWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLA 163
            W LN A Q+L  IL D GFDVW+GN RGTR+S  H  L+  ++ FWNW+W+EL  YDL 
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 164 EMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVR 223
            M ++I+ LT +KI+ +GHS GT++ FA+ ++  +  +V +AA+LSP++YL H+T  +  
Sbjct: 171 AMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGD 230

Query: 224 LMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDW 283
           +   T L       G+ E N         P S  V D I +  +  G             
Sbjct: 231 IAAKTFLAEATSILGWPEFN---------PKSGLVGDFIKAICLKAG------------- 290

Query: 284 GTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKG 343
                       ++C +++S ITG+NCCLN S  DLF   EP  +S KN+ HL Q +R  
Sbjct: 291 ------------IDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTVRDK 350

Query: 344 SFSKYDYGLL-KNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVK 403
              KY+YG   +N++ YGQ +PP +++S IP  LPL+ +YGG D L+D  D+E  + + K
Sbjct: 351 ELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFK 410

Query: 404 ----SVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFK 435
                   + ++++Y H DFI+ + AK+ VY+ +  FFK
Sbjct: 411 YHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of CsaV3_7G008200 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.4e-52
Identity = 130/395 (32.91%), Postives = 206/395 (52.15%), Query Frame = 0

Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEK-QKGPPILLLHGLFMAGDGWFL 105
           SQ++   GYPC E+++ T+DG++LG+ R+       E   K P + L HGL  +   W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N    SL F+L D G+DVW+GN RG  WS  +   S D  EFW +S++E+A YDL   IN
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  +KI+ VGHSQGT + F A  T P +AKK+     L+P++ +++  +PL ++ 
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKI- 214

Query: 226 VDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDW-G 285
                                         +F+    F F ++ G   ++   YF D+ G
Sbjct: 215 ------------------------------SFIP--TFLFKLMFGKKMFLPHTYFDDFLG 274

Query: 286 T-----VLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQM 345
           T      +LD LC    N + I      +N  LN SRFD++  + P  +S ++  H  Q+
Sbjct: 275 TEVCSREVLDLLCS---NTLFIFCGFDKKN--LNVSRFDVYLGHNPAGTSVQDFLHWAQL 334

Query: 346 IRKGSFSKYDYGL-LKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTI 405
           +R G F  +++G   +N+  Y Q+ PPE+D+S +   + +W   GGND L+D  D+   +
Sbjct: 335 VRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPVAVW--NGGNDILADPQDVAMLL 389

Query: 406 KKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 431
            K+ ++     +  Y H+DFI +M A ++VY+ MI
Sbjct: 395 PKLSNLLFHKEILAYNHLDFIWAMDAPQEVYNEMI 389

BLAST of CsaV3_7G008200 vs. ExPASy Swiss-Prot
Match: Q9CPP7 (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.6e-48
Identity = 129/393 (32.82%), Postives = 203/393 (51.65%), Query Frame = 0

Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEK-QKGPPILLLHGLFMAGDGWFL 105
           SQ++   GYP  E+++ T+DG++LG+ R+       E   K P   L HGL  +   W  
Sbjct: 35  SQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKRPVAYLQHGLIASATNWIT 94

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N    SL FIL D G+DVW+GN RG  WS  +   S D  EFW +S++E+A YDL   I+
Sbjct: 95  NLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATID 154

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  +KI+ VGHSQGT + F A  T P +AKK+     L+P++ +++  +P     
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESP----- 214

Query: 226 VDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSD-WG 285
                             FK++S     I  F++  IF    +   H Y+     ++   
Sbjct: 215 ------------------FKKISL----IPKFLLKVIFG-NKMFMPHNYLDQFLGTEVCS 274

Query: 286 TVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGS 345
             LLD LC    N + I      +N  LN SRFD++  + P  +S ++L H  Q+ + G 
Sbjct: 275 RELLDLLCS---NALFIFCGFDKKN--LNVSRFDVYLGHNPAGTSTQDLFHWAQLAKSGK 334

Query: 346 FSKYDYGL-LKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKS 405
              Y++G  L+N+  Y Q+ PP +D+S +   + +W   GG+D L+D  D+   + K   
Sbjct: 335 LQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVW--NGGHDILADPQDVAMLLPK--- 389

Query: 406 VPELVYLEN---YGHVDFILSMKAKEDVYDPMI 431
           +P L+Y +    Y H+DFI +M A ++VY+ ++
Sbjct: 395 LPNLLYHKEILPYNHLDFIWAMDAPQEVYNEIV 389

BLAST of CsaV3_7G008200 vs. ExPASy Swiss-Prot
Match: Q8K2A6 (Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.6e-48
Identity = 120/393 (30.53%), Postives = 198/393 (50.38%), Query Frame = 0

Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLL-HGLFMAGDGWFL 105
           S+++   GYP  E+++ T+DG++L + R+      L+K+   P++LL HGL      W  
Sbjct: 50  SEIIKHKGYPSEEYEVATEDGYILSVNRIPRGQTRLKKEGSRPVVLLQHGLLGDASNWIS 109

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N    SLGFIL D GFDVW+GN RG  WS  H +LS D+ EFW +S++E+A +DL  +IN
Sbjct: 110 NLPNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVIN 169

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  KK+Y VG+SQGT M F A  T P++A K+     L+PI+ +++  +P  +  
Sbjct: 170 FILQKTGQKKVYYVGYSQGTTMGFIAFSTMPELAHKIKMYFALAPIATVKYARSPGTKF- 229

Query: 226 VDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGT 285
                  ++L     ++ F R                F +        ++++C     G 
Sbjct: 230 -------LLLPDMMIKVLFGRQE--------------FLYQTRFFRQLFIYLC-----GQ 289

Query: 286 VLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSF 345
           ++LD +C    N I +L      N  +N SR +++  + P  +S +N+ H  Q +  G  
Sbjct: 290 MILDQICS---NIILLLGGFNTNN--MNMSRANVYVAHTPAGTSVQNILHWSQAVNSGEL 349

Query: 346 SKYDYGL-LKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSV 405
             +D+G   KN     Q  P  + +  +     +W   GG D LS+  D++  + +V ++
Sbjct: 350 RAFDWGSETKNQEKCNQPTPIRYKVRDMMVPTAMWT--GGQDWLSNPDDVKTLLSEVTNL 408

Query: 406 PELVYLENYGHVDFILSMKAKEDVYDPMIKFFK 435
                +  + HVDFI  + A + VY+ +I   K
Sbjct: 410 IYHKNIPEWAHVDFIWGLDAPQRVYNEIIHLMK 408

BLAST of CsaV3_7G008200 vs. ExPASy TrEMBL
Match: A0A0A0K4L2 (Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 7.7e-234
Identity = 407/442 (92.08%), Postives = 407/442 (92.08%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCINILS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINILS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV
Sbjct: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 407

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 407

BLAST of CsaV3_7G008200 vs. ExPASy TrEMBL
Match: A0A5A7SJL6 (Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 9.5e-224
Identity = 386/442 (87.33%), Postives = 401/442 (90.72%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFF+YEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQR+
Sbjct: 301 SITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRM 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+TIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMK 407

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 407

BLAST of CsaV3_7G008200 vs. ExPASy TrEMBL
Match: A0A1S3C115 (Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 9.5e-224
Identity = 386/442 (87.33%), Postives = 401/442 (90.72%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLDNLCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDNLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLNRSRFDLFF+YEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQR+
Sbjct: 301 SITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRM 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+TIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMK 407

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKFFKSLGKSSSL
Sbjct: 421 AKEDVYDPMIKFFKSLGKSSSL 407

BLAST of CsaV3_7G008200 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 4.9e-204
Identity = 355/442 (80.32%), Postives = 384/442 (86.88%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S ++YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRS-LEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSEDEKEFW+WSWEELALYDLA MI+YINSLT +K+Y+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIA+KV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLD+LCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDDLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLN SRFDLFF+YEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR 
Sbjct: 301 SITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRK 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TIK++KSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMK 406

Query: 421 AKEDVYDPMIKFFKSLGKSSSL 443
           AKEDVYDPMIKF KSLGKS +L
Sbjct: 421 AKEDVYDPMIKFLKSLGKSGNL 406

BLAST of CsaV3_7G008200 vs. ExPASy TrEMBL
Match: A0A6J1GL56 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.4e-203
Identity = 354/439 (80.64%), Postives = 382/439 (87.02%), Query Frame = 0

Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S ++YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRS-LEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSEDEKEFW+WSWEELALYDLA MI+YINSLT +K+Y+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIA+KV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300
           FK                                   SDWGTVLLD+LCDRLVNCIN+LS
Sbjct: 241 FK-----------------------------------SDWGTVLLDDLCDRLVNCINVLS 300

Query: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
           SITGENCCLN SRFDLFF+YEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR 
Sbjct: 301 SITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRK 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420
           PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TIK++KSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMK 403

Query: 421 AKEDVYDPMIKFFKSLGKS 440
           AKEDVYDPMIKF KSLGKS
Sbjct: 421 AKEDVYDPMIKFLKSLGKS 403

BLAST of CsaV3_7G008200 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 481.9 bits (1239), Expect = 5.6e-136
Identity = 234/415 (56.39%), Postives = 296/415 (71.33%), Query Frame = 0

Query: 27  SVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKG 86
           S +  S L H  S   SLC+ L+ PA Y C EH IQTKDG++L LQRV+S    L  Q G
Sbjct: 16  SALTQSHLLHG-SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSG 75

Query: 87  PPILLLHGLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEF 146
           PP+LL HGLFMAGD WFLNS ++SLGFIL D+GFDVW+GNVRGTR+SYGH +LS+ +KEF
Sbjct: 76  PPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEF 135

Query: 147 WNWSWEELALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLS 206
           W+WSW++LA+YDLAEMI Y+ S++N KI++VGHSQGTIMSFAALTQP +A+ V AAALL 
Sbjct: 136 WDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLC 195

Query: 207 PISYLEHITAPLVRLMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVH 266
           PISYL+H+TAPLV  MV  HLD +++A G H++NF+                        
Sbjct: 196 PISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFR------------------------ 255

Query: 267 GTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSS 326
                      SD    L+D+LC+  ++C + L+SITG NCC N S+ + +  YEPHPSS
Sbjct: 256 -----------SDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSS 315

Query: 327 AKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELS 386
            KN+ HLFQMIRKG+F++YDYG  KNLR YG   PPEF LS IP SLP+WM YGG D L+
Sbjct: 316 VKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLA 375

Query: 387 DWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSS 442
           D TD+E+T+ ++ S PEL+YLE+YGH+DF+L   AKEDVY  MI+FF++  KSSS
Sbjct: 376 DVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFFRAKVKSSS 392

BLAST of CsaV3_7G008200 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 282.0 bits (720), Expect = 8.5e-76
Identity = 148/399 (37.09%), Postives = 226/399 (56.64%), Query Frame = 0

Query: 44  LCSQLVLPAGYPCAEHKIQTKDGFLLGLQRV-SSRDGEL--EKQKGPPILLLHGLFMAGD 103
           +C+  V   GY C EH + T+DG++L +QR+   R G +  +  K  P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 104 GWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLA 163
            W LN A Q+L  IL D GFDVW+GN RGTR+S  H  L+  ++ FWNW+W+EL  YDL 
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 164 EMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVR 223
            M ++I+ LT +KI+ +GHS GT++ FA+ ++  +  +V +AA+LSP++YL H+T  +  
Sbjct: 171 AMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGD 230

Query: 224 LMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDW 283
           +   T L       G+ E N         P S  V D I +  +  G             
Sbjct: 231 IAAKTFLAEATSILGWPEFN---------PKSGLVGDFIKAICLKAG------------- 290

Query: 284 GTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKG 343
                       ++C +++S ITG+NCCLN S  DLF   EP  +S KN+ HL Q +R  
Sbjct: 291 ------------IDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTVRDK 350

Query: 344 SFSKYDYGLL-KNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVK 403
              KY+YG   +N++ YGQ +PP +++S IP  LPL+ +YGG D L+D  D+E  + + K
Sbjct: 351 ELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFK 410

Query: 404 ----SVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFK 435
                   + ++++Y H DFI+ + AK+ VY+ +  FFK
Sbjct: 411 YHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of CsaV3_7G008200 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.9e-15
Identity = 97/410 (23.66%), Postives = 160/410 (39.02%), Query Frame = 0

Query: 34  LPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLH 93
           L ++++ +   C  ++   GYP    ++ T DG++L L+R+  RD          + L H
Sbjct: 285 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDAR------KAVFLQH 344

Query: 94  GLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEE 153
           G+  +  GW  N    S  F   D G+DV++GN RG   S  H + +   KEFW +S  E
Sbjct: 345 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINE 404

Query: 154 LALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEH 213
               D+  MI  I+ +   ++               L QP+I +++         +    
Sbjct: 405 HGTEDIPAMIEKIHEIKTTEL--------------KLCQPNIDEEINQEEPYKLCAICHS 464

Query: 214 ITAPLVRLMVDT--------HLDTIILAS--GFHE---LNFKRLSCYAVPISNFVMDCIF 273
           +    + + V T         L  +IL S  GFHE   L F  +    + IS  +   + 
Sbjct: 465 LGGAAILMYVITRKIKEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVP 524

Query: 274 SFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVN-------CINILSSITGENCCLNRSR 333
           +F        Y+   +F     +LL+ L     N          ++S + G +       
Sbjct: 525 AF--------YIPTRFF----RMLLNKLARDFHNYPALGGLVQTLMSYVVGGDSSNWVGV 584

Query: 334 FDL-FFKYEPHPS-SAKNLHHLFQMIRKGSFSKYDYGLLK-NLRVYGQRVPPEFDLSRIP 393
             L  +     P+ S +   HL Q+   G F  YDYG    N+ VYG   P +   S   
Sbjct: 585 LGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEPLDLGESYKF 644

Query: 394 ESLPLWMAYGGNDEL--SDWTDLENTIKKVKSVPELVYLENYGHVDFILS 419
             +P+ +  G ND++  S        + +   V        Y H+DF  S
Sbjct: 645 IDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHLDFTFS 661

BLAST of CsaV3_7G008200 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.9e-15
Identity = 97/410 (23.66%), Postives = 160/410 (39.02%), Query Frame = 0

Query: 34  LPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLH 93
           L ++++ +   C  ++   GYP    ++ T DG++L L+R+  RD          + L H
Sbjct: 216 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDAR------KAVFLQH 275

Query: 94  GLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEE 153
           G+  +  GW  N    S  F   D G+DV++GN RG   S  H + +   KEFW +S  E
Sbjct: 276 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINE 335

Query: 154 LALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEH 213
               D+  MI  I+ +   ++               L QP+I +++         +    
Sbjct: 336 HGTEDIPAMIEKIHEIKTTEL--------------KLCQPNIDEEINQEEPYKLCAICHS 395

Query: 214 ITAPLVRLMVDT--------HLDTIILAS--GFHE---LNFKRLSCYAVPISNFVMDCIF 273
           +    + + V T         L  +IL S  GFHE   L F  +    + IS  +   + 
Sbjct: 396 LGGAAILMYVITRKIKEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVP 455

Query: 274 SFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVN-------CINILSSITGENCCLNRSR 333
           +F        Y+   +F     +LL+ L     N          ++S + G +       
Sbjct: 456 AF--------YIPTRFF----RMLLNKLARDFHNYPALGGLVQTLMSYVVGGDSSNWVGV 515

Query: 334 FDL-FFKYEPHPS-SAKNLHHLFQMIRKGSFSKYDYGLLK-NLRVYGQRVPPEFDLSRIP 393
             L  +     P+ S +   HL Q+   G F  YDYG    N+ VYG   P +   S   
Sbjct: 516 LGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEPLDLGESYKF 575

Query: 394 ESLPLWMAYGGNDEL--SDWTDLENTIKKVKSVPELVYLENYGHVDFILS 419
             +P+ +  G ND++  S        + +   V        Y H+DF  S
Sbjct: 576 IDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHLDFTFS 592

BLAST of CsaV3_7G008200 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 2.3e-12
Identity = 48/165 (29.09%), Postives = 75/165 (45.45%), Query Frame = 0

Query: 45  CSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFL 104
           C  ++   GYP    ++ T DG+ L L+R+  RD          + L HG+  +  GW  
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDAR------KAVYLQHGVMDSSMGWVS 353

Query: 105 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 164
           N    S  F   D G+DV++GN RG   S  H   +   K+FW +S  E A  D+  MI 
Sbjct: 354 NGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDIPAMIE 413

Query: 165 YINSLTNKKIYIVGHSQGTIMSFAALTQP----DIAKKVGAAALL 206
            I+ +   ++ +    Q T+       QP     ++  +G AA+L
Sbjct: 414 KIHEIKTSELKLY---QPTMEEVVNEDQPYKLCVVSHSLGGAAVL 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8645852.19.0e-261100.00hypothetical protein Csa_017220 [Cucumis sativus][more]
XP_004136959.11.6e-23392.08triacylglycerol lipase 1 [Cucumis sativus][more]
XP_008454984.12.0e-22387.33PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 tria... [more]
XP_038886839.18.0e-20981.90triacylglycerol lipase 1 isoform X1 [Benincasa hispida][more]
XP_023553816.12.7e-20481.09triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q71DJ57.9e-13556.39Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Q67ZU11.2e-7437.09Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
P046348.4e-5232.91Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Q9CPP79.6e-4832.82Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1[more]
Q8K2A69.6e-4830.53Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K4L27.7e-23492.08Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1[more]
A0A5A7SJL69.5e-22487.33Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=... [more]
A0A1S3C1159.5e-22487.33Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1[more]
A0A6J1GLC04.9e-20480.32Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1GL562.4e-20380.64Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G15230.15.6e-13656.39lipase 1 [more]
AT5G14180.18.5e-7637.09Myzus persicae-induced lipase 1 [more]
AT1G73920.12.9e-1523.66alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.22.9e-1523.66alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.12.3e-1229.09alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 260..438
e-value: 7.2E-44
score: 148.2
coord: 1..273
e-value: 1.2E-75
score: 252.8
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 47..105
e-value: 2.1E-16
score: 59.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 35..435
e-value: 3.8E-109
score: 367.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 46..434
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 36..243
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 267..436
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 267..436
coord: 36..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G008200.1CsaV3_7G008200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016788 hydrolase activity, acting on ester bonds