CsaV3_6G051480 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G051480
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionTwo-pore potassium channel 3
Locationchr6: 29949293 .. 29952784 (+)
RNA-Seq ExpressionCsaV3_6G051480
SyntenyCsaV3_6G051480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAGAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGGTACGCATTAAGATTATTCATTCAAACCCATTTAGAGAAGTTGCTTGGAAAATTGGATTTGATGTTACTGATTCTGATTGCCGAGAAGTTTTGCTTGCATTGTTTCATGAATATGCATGAGTATTTGTTGCATAAGTTTTATGAAACTGCCCATCTATTCTGGTATGTTTGAAGAGCGAGCCATTGGTTGAAAAAATCACGTTTTAGTTGTGCATTCATGGTATCCCTCTTATGAATATGATCATATTAATGATTCTATGAGACTTTCTACTTTTATAGCATCCAACTCAGCCTTCTGTTTGGCATAGGTTTGATATTATTTATCAACTCAGTCTTCCCTCTCCCAAGTACCACGAACTAACATATACAAAAAAGGATACTCCCCCTCCGACTTTGTCTCTCTCTCTTTTGGCATCCAGTATCCCAACTCATGCCCATTATGCAATCTCAAAATCTTGTTGACAGTAGATTCTTCAAAATTATTACATTCAAGCCAACTCCCTCTCTGAGGCGGTACCATTTTCTAATAAATGGTCTATTAATAAAACATGCTAATTATATGAATCTTTTAGATAGAACGGAAGAAGGATTTTTTTTTTCCTTTTGTTTTTAATGAAAGGGTAGATACTTCGGACTTGGTTCATTTGAAGACTTCTACTTGGTGTATCACCTACACTTTTGCAATAATGATTCTCTTGTTTGATTCTGATATGACAATAATCAAAACTCATGTAGATGTAGAGAGGGTGTCTTCAAAGACTATAGACTTTGCAGAGGAAAATGTTGGGATACCAGTAGGGAAGTATTAAAAATTATTCCTTAGTGATTAGTTGGTAAATTTGTTAGAAGTGAATGGTTATAGACGGAGTCAGGGAACAGAATGTAGGCAAATTTTAAGAGAATGTAGGCAAATTTTAAGAGAATGTAGATGTCTCGGAGTGCTTAGAACTTCATAAATTTTTCTAACGTTAATAGCATATTTCCATTAGTTCTGCTCCTGAGCAGAAGTACTATACAGTTATTGTTTAGTCATGGGAAGATATTGTACAAATAAAAATTAGAAGAGCGTAGAAGGTCGTGAAAGCCTCCTCCTCAAACTATTCTAAATAGAAATCACCTCAGTTCGAAGCCGCATTTCATCAATATGTGATTTAACTGTTCAGAATGTGTTAGGCCACCAATAGTGCATCAGTATAGTAGGAGAGGAAAGAATAATAAGGGCAAGGGAATTGTAAGGGGTGAGGACCACTTAGGGAGAATAATATAAATATGGTCAAAGTACATTGAGTTAGTTAGAAATTGCCTAGTATCTTCTTGAGAGTGAGAGATAGTTAGTTAGGGACGATTGTCTGTGATTGTTCCTAGTTATTCTACTGTGATTTCGGTGTACGATCCATTACTATTACAATAAAGATCGATACCTTTCAGAATGTAGGTACAAAAGTTTCGCAATTGAATGAAATTTGTGTGTGTCGTTTTTTTCTTAAGGTCCTGTCCTGGCTTCTGAATAGTAATTAGTTCTTCATTTGATACAATTTGAAGAAGTGGTAACTGAGAAATCATGATATATTTCTTTGCCATTAGTCTTCTGGTAGGACTCTTTTTTCTTTTAACAAGAAATGACGAGTAATGGGTTTCTTCTGTACATGTTTTCACTGATGACGTTTGTTGTGCACTTGGGCTAACCTGTTGGAATCTTATTACATGTTTGGATGGATTAGGATTACCATAGTAATTATGGTGGTGGATTCACCGAAGTGAACAAGACATAAGATCTTTACCTCAACCATTTTATATTCTCTGGATTTACTCTGATTGAAACTCTACAAGATGATCTTGATCATTCAAAATTGTACTTGATTCTTCTTTTAACGTGTTCTTTATTCAATCTGTAATTCCATCTCTAGTTTGTTTCTAGTGCCAGTTTGAGTTCCGGCTTTCCTTCCCAATCATCCTATCCTTTTCAATTCCCCATATTCTTCTGCCCTCTTAAATATCCTAGTCTAGCAAGTTTGGCTAGTAGCTCATTTGGTACGCATATCATGTTCTCAATCAAGTAATATAATTTATTCTCCTTGCCTTCTGATAGTTTCCATTTTACTAGCCTTGCAAGCTCTCTTGTGGATGAACTGTCTTCCCATTTAGTTTGACATATAACAGACCATCAAAACTTGGTGAGCTCACTTTGTATAGTTTATGTTATCCCTTTTGCAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATTTCTAATAACTTTGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGA

mRNA sequence

ATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAGAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGGTACGCATTAAGATTATTCATTCAAACCCATTTAGAGAAGTTGCTTGGAAAATTGGATTTGATGTTACTGATTCTGATTGCCGAGAAGTTTTGCTTGCATTGTTTCATGAATATGCATGAGTATTTGTTGCATAAGTTTTATGAAACTGCCCATCTATTCTGCATCCAACTCAGCCTTCTGTTTGGCATAGTATCCCAACTCATGCCCATTATGCAATCTCAAAATCTTGTTGACAGTAGATTCTTCAAAATTATTACATTCAAGCCAACTCCCTCTCTGAGGCGGGTAGATACTTCGGACTTGGTTCATTTGAAGACTTCTACTTGGTGTATCACCTACACTTTTGCAATAATGATTCTCTTGTTTGATTCTGATATGACAATAATCAAAACTCATGTAGATGTAGAGAGGGTGTCTTCAAAGACTATAGACTTTGCAGAGGAAAATGTTGGGATACCAGTAGGGAATGCCAGTTTGAGTTCCGGCTTTCCTTCCCAATCATCCTATCCTTTTCAATTCCCCATATTCTTCTGCCCTCTTAAATATCCTAGTCTAGCAAGTTTGGCTAGTAGCTCATTTGGTACGCATATCATGTTCTCAATCAATAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATTTCTAATAACTTTGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGA

Coding sequence (CDS)

ATGGAGAAGGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGTCTCCCATACCTCCTCAATTATGTCCACTTCCTGAAAACGATGAAATTACTCTGCCTATGACGCCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCTTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAACAGGCGTGGCTTGTGGATTCCAATTATACATGGCCCAAAAGCAACCTCCATCGCTCCAGAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAGTTTGGTAAACAGTCTATTATCCGCCAAGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTTCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGGTATGGAGATATTACCCCCAATAGTACGTCGACAAAGTTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTAGGTGTTGTGATCCTTTGTATAGGGGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCGACCCTTGCTGTTGCTCGGGCCTTCCTTTATTTAGCGGAGGCAAGAGTAGATAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCGTTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGGTACGCATTAAGATTATTCATTCAAACCCATTTAGAGAAGTTGCTTGGAAAATTGGATTTGATGTTACTGATTCTGATTGCCGAGAAGTTTTGCTTGCATTGTTTCATGAATATGCATGAGTATTTGTTGCATAAGTTTTATGAAACTGCCCATCTATTCTGCATCCAACTCAGCCTTCTGTTTGGCATAGTATCCCAACTCATGCCCATTATGCAATCTCAAAATCTTGTTGACAGTAGATTCTTCAAAATTATTACATTCAAGCCAACTCCCTCTCTGAGGCGGGTAGATACTTCGGACTTGGTTCATTTGAAGACTTCTACTTGGTGTATCACCTACACTTTTGCAATAATGATTCTCTTGTTTGATTCTGATATGACAATAATCAAAACTCATGTAGATGTAGAGAGGGTGTCTTCAAAGACTATAGACTTTGCAGAGGAAAATGTTGGGATACCAGTAGGGAATGCCAGTTTGAGTTCCGGCTTTCCTTCCCAATCATCCTATCCTTTTCAATTCCCCATATTCTTCTGCCCTCTTAAATATCCTAGTCTAGCAAGTTTGGCTAGTAGCTCATTTGGTACGCATATCATGTTCTCAATCAATAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAAGGTATCAGAGAAAGATGTCATGCAGATTTCTAATAACTTTGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTGA

Protein sequence

MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLLHKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVHLKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGFPSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLESHH*
Homology
BLAST of CsaV3_6G051480 vs. NCBI nr
Match: KAE8647742.1 (hypothetical protein Csa_004248 [Cucumis sativus])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 627/627 (100.00%), Postives = 627/627 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL
Sbjct: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
           HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH
Sbjct: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
           LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF
Sbjct: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
           PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM
Sbjct: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLDSGKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDSGKCGKITLADLLESHH 627

BLAST of CsaV3_6G051480 vs. NCBI nr
Match: XP_004134597.1 (two-pore potassium channel 3 [Cucumis sativus])

HSP 1 Score: 745.7 bits (1924), Expect = 3.2e-211
Identity = 424/627 (67.62%), Postives = 425/627 (67.78%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           TVSEFLAADIDNNGFV                                            
Sbjct: 361 TVSEFLAADIDNNGFV-------------------------------------------- 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 425

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 425

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
                                                 +RSEYVIYKLKEMGKVSEKDVM
Sbjct: 541 --------------------------------------SRSEYVIYKLKEMGKVSEKDVM 425

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLDSGKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDSGKCGKITLADLLESHH 425

BLAST of CsaV3_6G051480 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 736.1 bits (1899), Expect = 2.5e-208
Identity = 417/627 (66.51%), Postives = 423/627 (67.46%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           T+SEFLAADIDNNGFV                                            
Sbjct: 361 TISEFLAADIDNNGFV-------------------------------------------- 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 425

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 425

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
                                                 +RSEYVIYKLKEMG+VSEKDVM
Sbjct: 541 --------------------------------------SRSEYVIYKLKEMGRVSEKDVM 425

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLDSGKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDSGKCGKITLADLLESHH 425

BLAST of CsaV3_6G051480 vs. NCBI nr
Match: KAA0052565.1 (two-pore potassium channel 3 [Cucumis melo var. makuwa] >TYK13260.1 two-pore potassium channel 3 [Cucumis melo var. makuwa])

HSP 1 Score: 735.7 bits (1898), Expect = 3.3e-208
Identity = 378/389 (97.17%), Postives = 383/389 (98.46%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLE 390
           T+SEFLAADIDNNGFVRYAL LFI +  E
Sbjct: 361 TISEFLAADIDNNGFVRYALTLFIHSFRE 389

BLAST of CsaV3_6G051480 vs. NCBI nr
Match: XP_038883350.1 (two-pore potassium channel 3-like [Benincasa hispida])

HSP 1 Score: 716.5 bits (1848), Expect = 2.1e-202
Identity = 408/627 (65.07%), Postives = 417/627 (66.51%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY+SPRGKP P P QLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYVSPRGKPPPPPAQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLD +SQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDLKSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGV IYWLNRD FSA ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVFIYWLNRDKFSAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSY+LDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYMLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           G++RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGM VMHFVENLGWLDSFYLSVM
Sbjct: 241 GLQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMSVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           TVSEFLAADIDNNGFV                                            
Sbjct: 361 TVSEFLAADIDNNGFV-------------------------------------------- 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 425

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 425

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
                                                 +RSEYVIYKLKEMGKVSEKDVM
Sbjct: 541 --------------------------------------SRSEYVIYKLKEMGKVSEKDVM 425

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLD+GKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDTGKCGKITLADLLESHH 425

BLAST of CsaV3_6G051480 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 6.8e-124
Identity = 253/386 (65.54%), Postives = 305/386 (79.02%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPSPIPPQLC-PLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASP 63
           +PLL Y +SPR K    PPQL  PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPRLKK---PPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSR 123
             D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRS+
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADIDNNGFVRYA 380
            +L + M+VS+F AADIDNNG V  A
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKA 388


HSP 2 Score: 62.8 bits (151), Expect = 1.6e-08
Identity = 28/47 (59.57%), Postives = 38/47 (80.85%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLE 625
           ++++EYVIYKLKEM K+++KD++ IS  FD+LD    GKITL DLLE
Sbjct: 385 VSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLLE 431

BLAST of CsaV3_6G051480 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.1e-110
Identity = 227/386 (58.81%), Postives = 290/386 (75.13%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPSPIPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR   +P PP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPR--INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRS+TAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD+++ ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFVRYA 380
            +LG+ M++S+FL ADID NG V  A
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKA 388


HSP 2 Score: 58.9 bits (141), Expect = 2.3e-07
Identity = 26/48 (54.17%), Postives = 37/48 (77.08%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLES 626
           ++++E+VIYKLK+M K++EKD+  I   FD+LD    G+ITL DLLES
Sbjct: 385 VSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDLLES 432

BLAST of CsaV3_6G051480 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.5e-94
Identity = 218/434 (50.23%), Postives = 279/434 (64.29%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITL---PMTP-----TEFKDRLIFG---- 60
           M+ EPLL  LSP       P  L PLPE+ E++    P++P     + +K+R+IFG    
Sbjct: 1   MDTEPLLSPLSPS------PHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHPP 60

Query: 61  ---PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTW 120
              P   P    P        +S       S    PS P D  +   +  +     ++  
Sbjct: 61  PPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNPPSLF---DFIG 120

Query: 121 PKSNLHRSRTAPAMAVINDV--------NHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVL 180
            ++NLHRSRTAPAMA +N            S+ P P   + +I+  A + L+ YL++GV 
Sbjct: 121 GRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLAMGVT 180

Query: 181 IYWLNRDNF--SAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFI 240
            Y     NF  SA  THPV DALYFCIVT+CTIGYGDITP + + KLFS+ FVL+GFGF+
Sbjct: 181 FYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGFGFV 240

Query: 241 DILLSGMVSYVLDLQESYLLRNVK--RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVI 300
           DILLSGMVSYVLDLQE  L+  +K  R V R+   +YI D+KKGRMR+RMKVALAL VV 
Sbjct: 241 DILLSGMVSYVLDLQEHLLITALKNPRSV-RKHRHNYIFDLKKGRMRVRMKVALALTVVA 300

Query: 301 LCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVA 360
           +C+GVG  V+  VENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVSTLAVA
Sbjct: 301 ICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLAVA 360

Query: 361 RAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFVRYALRLFIQTHLEKLLG 408
           RAFLYLAE R+DKRHR MA W+L +DMTVSEFLAADIDNNG+V  +   F+   L K +G
Sbjct: 361 RAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKS--EFVVYKL-KEMG 420

BLAST of CsaV3_6G051480 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.2e-89
Identity = 194/345 (56.23%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 49  PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKS 108
           PSSS   AS     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSRTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIVYLSLGVLI 168
           N        LHRSRTAPAM +I D+     +  KP    +SIIRQA+ LLIVYL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDIL 228
           Y  NRD++S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V+FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVK-------RGVKRESGKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++           R S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIGVG  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFV 377
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFI 359


HSP 2 Score: 58.9 bits (141), Expect = 2.3e-07
Identity = 25/46 (54.35%), Postives = 37/46 (80.43%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLL 624
           I++SEY++ KLKEMGK+++KD+ Q+   F++LD  + GKITL DLL
Sbjct: 359 ISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404

BLAST of CsaV3_6G051480 vs. ExPASy Swiss-Prot
Match: Q9FWX6 (Two-pore potassium channel 4 OS=Arabidopsis thaliana OX=3702 GN=TPK4 PE=2 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 1.5e-46
Identity = 105/237 (44.30%), Postives = 155/237 (65.40%), Query Frame = 0

Query: 110 LHRSRTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQ 169
           LH + ++P    +  V+ S        K +I+  A++LL+VYL+ GV  Y   RD FS  
Sbjct: 12  LHPNESSPEETQVTTVSKS--------KWTILVLAMILLLVYLTFGVCTYSFFRDQFSGT 71

Query: 170 ETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDL 229
           ET+  VDA YF IVT  T+GYGDI P++++TK+ +++ V  G  F+D LL+ +VS+VL L
Sbjct: 72  ETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTIVLVSTGVVFLDYLLNRVVSHVLSL 131

Query: 230 QESYLLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENL 289
           QE+ +L  + +  +  + + +I   + G++R++ K+ LA   V LC+G G   +H  E L
Sbjct: 132 QENAILDRINK-TRNRAIRDHI--AEDGKIRLKWKLCLAFCAVGLCVGSGALFLHVFERL 191

Query: 290 GWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDK 347
            WLDS YLSV+SVTTVGYGD+ FK++ GR FA  WLL+ST+A+A  FLYLAE R+D+
Sbjct: 192 DWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLSTIAMATLFLYLAEMRIDR 237

BLAST of CsaV3_6G051480 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 1.2e-208
Identity = 417/627 (66.51%), Postives = 423/627 (67.46%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           T+SEFLAADIDNNGFV                                            
Sbjct: 361 TISEFLAADIDNNGFV-------------------------------------------- 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 425

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 425

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
                                                 +RSEYVIYKLKEMG+VSEKDVM
Sbjct: 541 --------------------------------------SRSEYVIYKLKEMGRVSEKDVM 425

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLDSGKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDSGKCGKITLADLLESHH 425

BLAST of CsaV3_6G051480 vs. ExPASy TrEMBL
Match: A0A5A7UFS5 (Two-pore potassium channel 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008780 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.6e-208
Identity = 378/389 (97.17%), Postives = 383/389 (98.46%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLE 390
           T+SEFLAADIDNNGFVRYAL LFI +  E
Sbjct: 361 TISEFLAADIDNNGFVRYALTLFIHSFRE 389

BLAST of CsaV3_6G051480 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 7.4e-198
Identity = 400/628 (63.69%), Postives = 413/628 (65.76%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRS+TAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYL 420
           MTVSEFLAAD DNNG                                             
Sbjct: 361 MTVSEFLAADTDNNG--------------------------------------------- 420

Query: 421 LHKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLV 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 426

Query: 481 HLKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSG 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 426

Query: 541 FPSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDV 600
                                                 ++RSEYV+YKLKEMGKVSEKDV
Sbjct: 541 -------------------------------------CVSRSEYVLYKLKEMGKVSEKDV 426

Query: 601 MQISNNFDRLDSGKCGKITLADLLESHH 628
           MQISNNFDRLD+G CGKITLADLLESHH
Sbjct: 601 MQISNNFDRLDTGNCGKITLADLLESHH 426

BLAST of CsaV3_6G051480 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 4.8e-197
Identity = 399/628 (63.54%), Postives = 412/628 (65.61%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP P PPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRS+TAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLI+YLSLGVLIYW+NRD FSA ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYL 420
           MTVSEFLAAD DNNG                                             
Sbjct: 361 MTVSEFLAADTDNNG--------------------------------------------- 420

Query: 421 LHKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLV 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 426

Query: 481 HLKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSG 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 426

Query: 541 FPSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDV 600
                                                 ++RSEYV+YKLKEMGKVSEKDV
Sbjct: 541 -------------------------------------CVSRSEYVLYKLKEMGKVSEKDV 426

Query: 601 MQISNNFDRLDSGKCGKITLADLLESHH 628
           MQISNNFDRLD+G CGKITLADLLESHH
Sbjct: 601 MQISNNFDRLDTGNCGKITLADLLESHH 426

BLAST of CsaV3_6G051480 vs. ExPASy TrEMBL
Match: A0A6J1CLR2 (two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 1.2e-195
Identity = 397/627 (63.32%), Postives = 410/627 (65.39%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY SPR K  P  P LCPLPENDEITLP+TPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYQSPRAKLPPPIPLLCPLPENDEITLPLTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120
           DALTLSLSSSR S SSSL DPSTPLDP+SQSQSQQAWLVDSNY WPK+NLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRSSPSSSLYDPSTPLDPKSQSQSQQAWLVDSNYAWPKTNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180
           VINDVNHSQE KPQFG QSIIRQAVVLLI+YLSLGVLIYW+N DNF+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQERKPQFGNQSIIRQAVVLLIIYLSLGVLIYWINLDNFTAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKS+TGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSLTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFVRYALRLFIQTHLEKLLGKLDLMLLILIAEKFCLHCFMNMHEYLL 420
           TVSEFLAADIDNNGFV                                            
Sbjct: 361 TVSEFLAADIDNNGFV-------------------------------------------- 420

Query: 421 HKFYETAHLFCIQLSLLFGIVSQLMPIMQSQNLVDSRFFKIITFKPTPSLRRVDTSDLVH 480
                                                                       
Sbjct: 421 ------------------------------------------------------------ 425

Query: 481 LKTSTWCITYTFAIMILLFDSDMTIIKTHVDVERVSSKTIDFAEENVGIPVGNASLSSGF 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 425

Query: 541 PSQSSYPFQFPIFFCPLKYPSLASLASSSFGTHIMFSINRSEYVIYKLKEMGKVSEKDVM 600
                                                 +RSEYVIYKLKEMGKVSEKDVM
Sbjct: 541 --------------------------------------SRSEYVIYKLKEMGKVSEKDVM 425

Query: 601 QISNNFDRLDSGKCGKITLADLLESHH 628
           QISNNFDRLD+GKCGKITLADLLESHH
Sbjct: 601 QISNNFDRLDTGKCGKITLADLLESHH 425

BLAST of CsaV3_6G051480 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.8e-125
Identity = 253/386 (65.54%), Postives = 305/386 (79.02%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPSPIPPQLC-PLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASP 63
           +PLL Y +SPR K    PPQL  PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPRLKK---PPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSR 123
             D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRS+
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADIDNNGFVRYA 380
            +L + M+VS+F AADIDNNG V  A
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKA 388


HSP 2 Score: 62.8 bits (151), Expect = 1.2e-09
Identity = 28/47 (59.57%), Postives = 38/47 (80.85%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLE 625
           ++++EYVIYKLKEM K+++KD++ IS  FD+LD    GKITL DLLE
Sbjct: 385 VSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLLE 431

BLAST of CsaV3_6G051480 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 402.1 bits (1032), Expect = 8.0e-112
Identity = 227/386 (58.81%), Postives = 290/386 (75.13%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPSPIPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR   +P PP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPR--INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRS+TAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD+++ ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFVRYA 380
            +LG+ M++S+FL ADID NG V  A
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKA 388


HSP 2 Score: 58.9 bits (141), Expect = 1.7e-08
Identity = 26/48 (54.17%), Postives = 37/48 (77.08%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLLES 626
           ++++E+VIYKLK+M K++EKD+  I   FD+LD    G+ITL DLLES
Sbjct: 385 VSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDLLES 432

BLAST of CsaV3_6G051480 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 330.5 bits (846), Expect = 3.0e-90
Identity = 194/345 (56.23%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 49  PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKS 108
           PSSS   AS     +T+  S S  S    +      L  +   QS Q    D +    ++
Sbjct: 20  PSSSSSSAS-----ITIPRSISNTSFFHEISQERLLLHHQDLEQSVQDDKEDQDSDSDET 79

Query: 109 N--------LHRSRTAPAMAVINDVNHS--QEPKPQFGKQSIIRQAVVLLIVYLSLGVLI 168
           N        LHRSRTAPAM +I D+     +  KP    +SIIRQA+ LLIVYL+LGV I
Sbjct: 80  NRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKSIIRQAIFLLIVYLTLGVSI 139

Query: 169 YWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDIL 228
           Y  NRD++S  ETHPVVDALYFCIVTMCTIGYGDI P +  TK+F+V+FVL GFGF+DIL
Sbjct: 140 YSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDIL 199

Query: 229 LSGMVSYVLDLQESYLLRNVK-------RGVKRESGKSYIIDVKKGRMRIRMKVALALGV 288
           LSG+V+YVLDLQES +L  ++           R S K YIID +KGRMRIRMKV LAL V
Sbjct: 200 LSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCV 259

Query: 289 VILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLA 348
           V+LCIGVG  V+HFVE LG++DS YLSVMSVTTVGYGD+AFK++ GR+FA++WLLVSTLA
Sbjct: 260 VVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLA 319

Query: 349 VARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADIDNNGFV 377
           VARAFLYLAEAR+D+RHR   K  L +++TV + L AD   +GF+
Sbjct: 320 VARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFI 359


HSP 2 Score: 58.9 bits (141), Expect = 1.7e-08
Identity = 25/46 (54.35%), Postives = 37/46 (80.43%), Query Frame = 0

Query: 578 INRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADLL 624
           I++SEY++ KLKEMGK+++KD+ Q+   F++LD  + GKITL DLL
Sbjct: 359 ISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404

BLAST of CsaV3_6G051480 vs. TAIR 10
Match: AT1G02510.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 189.1 bits (479), Expect = 1.1e-47
Identity = 105/237 (44.30%), Postives = 155/237 (65.40%), Query Frame = 0

Query: 110 LHRSRTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQ 169
           LH + ++P    +  V+ S        K +I+  A++LL+VYL+ GV  Y   RD FS  
Sbjct: 12  LHPNESSPEETQVTTVSKS--------KWTILVLAMILLLVYLTFGVCTYSFFRDQFSGT 71

Query: 170 ETHPVVDALYFCIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDL 229
           ET+  VDA YF IVT  T+GYGDI P++++TK+ +++ V  G  F+D LL+ +VS+VL L
Sbjct: 72  ETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTIVLVSTGVVFLDYLLNRVVSHVLSL 131

Query: 230 QESYLLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENL 289
           QE+ +L  + +  +  + + +I   + G++R++ K+ LA   V LC+G G   +H  E L
Sbjct: 132 QENAILDRINK-TRNRAIRDHI--AEDGKIRLKWKLCLAFCAVGLCVGSGALFLHVFERL 191

Query: 290 GWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDK 347
            WLDS YLSV+SVTTVGYGD+ FK++ GR FA  WLL+ST+A+A  FLYLAE R+D+
Sbjct: 192 DWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLSTIAMATLFLYLAEMRIDR 237

BLAST of CsaV3_6G051480 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 172.2 bits (435), Expect = 1.4e-42
Identity = 115/325 (35.38%), Postives = 184/325 (56.62%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSRTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RSR+AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  S  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS++++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVRYALRLFIQTHLEKLLGKLD 397
            +G V  A   FI   L K +GK+D
Sbjct: 308 EDGVVGAA--EFIVYKL-KEMGKID 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8647742.10.0e+00100.00hypothetical protein Csa_004248 [Cucumis sativus][more]
XP_004134597.13.2e-21167.62two-pore potassium channel 3 [Cucumis sativus][more]
XP_008439665.12.5e-20866.51PREDICTED: two-pore potassium channel 3 [Cucumis melo][more]
KAA0052565.13.3e-20897.17two-pore potassium channel 3 [Cucumis melo var. makuwa] >TYK13260.1 two-pore pot... [more]
XP_038883350.12.1e-20265.07two-pore potassium channel 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SVV66.8e-12465.54Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Q9FL251.1e-11058.81Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q69TN41.5e-9450.23Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Q9S6Z84.2e-8956.23Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q9FWX61.5e-4644.30Two-pore potassium channel 4 OS=Arabidopsis thaliana OX=3702 GN=TPK4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3AYW01.2e-20866.51two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1[more]
A0A5A7UFS51.6e-20897.17Two-pore potassium channel 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1ED617.4e-19863.69two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 ... [more]
A0A6J1IME34.8e-19763.54two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE... [more]
A0A6J1CLR21.2e-19563.32two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441... [more]
Match NameE-valueIdentityDescription
AT4G18160.14.8e-12565.54Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.18.0e-11258.81Ca2+ activated outward rectifying K+ channel 2 [more]
AT4G01840.13.0e-9056.23Ca2+ activated outward rectifying K+ channel 5 [more]
AT1G02510.11.1e-4744.30Outward rectifying potassium channel protein [more]
AT5G55630.11.4e-4235.38Outward rectifying potassium channel protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 183..211
score: 35.03
coord: 303..312
score: 47.04
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 578..622
coord: 90..377
NoneNo IPR availableGENE3D1.10.287.70coord: 248..355
e-value: 3.0E-16
score: 61.3
NoneNo IPR availableGENE3D1.10.287.70coord: 137..237
e-value: 3.9E-21
score: 76.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..25
NoneNo IPR availablePANTHERPTHR11003:SF282TWO-PORE POTASSIUM CHANNEL 3coord: 578..622
coord: 90..377
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 255..359
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 140..226
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 272..340
e-value: 8.8E-11
score: 41.6
coord: 147..226
e-value: 1.4E-15
score: 57.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G051480.1CsaV3_6G051480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0005267 potassium channel activity