CsaV3_6G051240 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_6G051240 vs. NCBI nr
Match: XP_004134922.1 (subtilisin-like protease SBT5.6 isoform X2 [Cucumis sativus] >KGN49345.1 hypothetical protein Csa_003109 [Cucumis sativus])

HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 791/791 (100.00%), Postives = 791/791 (100.00%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

Query: 781 YVRSPIAVSST 792
           YVRSPIAVSST
Sbjct: 781 YVRSPIAVSST 791

BLAST of CsaV3_6G051240 vs. NCBI nr
Match: XP_031743664.1 (subtilisin-like protease SBT5.6 isoform X1 [Cucumis sativus])

HSP 1 Score: 1592.0 bits (4121), Expect = 0.0e+00
Identity = 791/813 (97.29%), Postives = 791/813 (97.29%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNR----------------------KIIGARYYLKGYEHHFGRLNKTADY 240
           QTGPAFQSAHCNR                      KIIGARYYLKGYEHHFGRLNKTADY
Sbjct: 181 QTGPAFQSAHCNRPQQPIGCHHRRLLVACSSCYTEKIIGARYYLKGYEHHFGRLNKTADY 240

Query: 241 RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA 300
           RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA
Sbjct: 241 RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA 300

Query: 301 LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSA 360
           LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSA
Sbjct: 301 LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSA 360

Query: 361 GNYGPTPSALSNVAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLV 420
           GNYGPTPSALSNVAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLV
Sbjct: 361 GNYGPTPSALSNVAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLV 420

Query: 421 YAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMIL 480
           YAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMIL
Sbjct: 421 YAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMIL 480

Query: 481 GNVPAVGRRPHADPHFVPATAVSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMA 540
           GNVPAVGRRPHADPHFVPATAVSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMA
Sbjct: 481 GNVPAVGRRPHADPHFVPATAVSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMA 540

Query: 541 NFSSRGPNPIDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSC 600
           NFSSRGPNPIDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSC
Sbjct: 541 NFSSRGPNPIDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSC 600

Query: 601 PHVSAAAALLRAIHPTWSQAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGH 660
           PHVSAAAALLRAIHPTWSQAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGH
Sbjct: 601 PHVSAAAALLRAIHPTWSQAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGH 660

Query: 661 FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRN 720
           FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRN
Sbjct: 661 FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRN 720

Query: 721 VVRIKRTVTNVGGGGKNVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNN 780
           VVRIKRTVTNVGGGGKNVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNN
Sbjct: 721 VVRIKRTVTNVGGGGKNVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNN 780

Query: 781 NRSSKKGEDYSFGWFAWSDGIHYVRSPIAVSST 792
           NRSSKKGEDYSFGWFAWSDGIHYVRSPIAVSST
Sbjct: 781 NRSSKKGEDYSFGWFAWSDGIHYVRSPIAVSST 813

BLAST of CsaV3_6G051240 vs. NCBI nr
Match: XP_008439697.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 [Cucumis melo])

HSP 1 Score: 1542.7 bits (3993), Expect = 0.0e+00
Identity = 764/793 (96.34%), Postives = 777/793 (97.98%), Query Frame = 0

Query: 1   MEKSSFLCHCR-LLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERH 60
           MEKSSFLCHC  LLLLLLLLLIG FIQQAASSSNNQKKAYIVYFGEHHGEKS+EEIKERH
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMET 120
           HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRMET
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
           TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWP SKSFSDKGMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRR 240
           CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
           VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360
           VDVLSLSIGKSEPYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420
           ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SLSHEKAKGK VLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA
Sbjct: 421 SLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540
           VSYEDAN+ILKYIKSR NPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA
Sbjct: 481 VSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600
           PGVDILAAWSEQDSPTKL KYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
           IRSALMTTSTTNNK GQPITDDST DNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL
Sbjct: 601 IRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660

Query: 661 HYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFF 720
           HYLCGLKMNSIDPSF CPPR LHPHDLNYPSIAVPQLR+VVRIKRTVTNVGGGGK+VYFF
Sbjct: 661 HYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFF 720

Query: 721 KSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVN--NNNRSSKKGEDYSFGWFAWSD 780
           KSEAP GVAVSASPNILYFNRVGERKKFTITISRKVN  +++R SKKGE+YSFGWFAWSD
Sbjct: 721 KSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAWSD 780

Query: 781 GIHYVRSPIAVSS 791
            IHYVRSPIAVS+
Sbjct: 781 EIHYVRSPIAVST 793

BLAST of CsaV3_6G051240 vs. NCBI nr
Match: XP_038882517.1 (subtilisin-like protease SBT5.6 [Benincasa hispida])

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 718/791 (90.77%), Postives = 748/791 (94.56%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           MEKSSF C   LLLL        FIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RHH
Sbjct: 1   MEKSSFFCFVLLLLL-------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRM TT
Sbjct: 61  SYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGNYGPTPSALSN+APWIITVGA
Sbjct: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLVYAGDI+ PH PRNQSGLCVAGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSHEKAKGKIVLCFRGEGISRFAGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SYEDAN+ILKYIKSR NPTA IVPP+TIYGSRPAPAMANFSSRGPNP+DPHFLKPDITAP
Sbjct: 481 SYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSEQDSPTKLPKYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTT+TT NK G P+TDD++ DNSPA+PFSFGSGHFRPSKAADPGLVYDSNYTDYLH
Sbjct: 601 RSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSIDPSF CPPRALHPHDLNYPSIAVP+LR  VRIKRTVTNVGGGGK+VYFF 
Sbjct: 661 YLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFH 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           SEAP GVAVSASPNILYFNRVGERK+FTIT+S KV N+N ++     YSFGWFAW+DGIH
Sbjct: 721 SEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSN-NNDNNNGYSFGWFAWTDGIH 780

Query: 781 YVRSPIAVSST 792
           YVRSPIAVSST
Sbjct: 781 YVRSPIAVSST 782

BLAST of CsaV3_6G051240 vs. NCBI nr
Match: XP_023517293.1 (subtilisin-like protease SBT5.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1340.1 bits (3467), Expect = 0.0e+00
Identity = 661/791 (83.57%), Postives = 717/791 (90.64%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           ME SS  C   LLLLLLLLL+        SS   QK+AYIVYFGEH G+KS EEI+ERHH
Sbjct: 1   METSSLFC---LLLLLLLLLL------PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLM VK++E DAKS LLY+YKH+IN FAA+LT QQASKLS+L+EVVSVIESK+Y M TT
Sbjct: 61  SYLMSVKDTENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKRYGMHTT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKP ++DLVS+A  GKDVVIGMLDSGVWPKSKSFSDKGMGP+PKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGP FQS HCNRKIIGARYY+KGYEHHFGRLN+T D+RSPCD DGHGSHTASIAGGRRV
Sbjct: 181 QTGPGFQSPHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGV
Sbjct: 241 YNVSAFGGVARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGN GPTPS LSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL R KMYPLVYA DI  PH PRN+SG C+AGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSH+KAKGKIVLC+RG+GISR+AGSLEV+RSGGAGMILG VPAVG R HADPHFVPATAV
Sbjct: 421 LSHKKAKGKIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SY+DAN ILKYIKS +NPTATIVPP+TIYGSRPAPAMA F+SRGP+ +DPHFLKPDITAP
Sbjct: 481 SYDDANRILKYIKSHRNPTATIVPPITIYGSRPAPAMAYFTSRGPSLVDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSE+DSPTKLPK  DPRIV+YN+YSGTSMSCPHVSAAAALLRAIHPTWSQAAI
Sbjct: 541 GVDILAAWSEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTT+TT NKYG PITDDST DNSPATPFSFGSGHFRP+KAADPGLVYD+NYTDYLH
Sbjct: 601 RSALMTTATTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIA+P+L+  VRIKRTVTNVGGGGK VYFF+
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKTVYFFE 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           S AP GVAV ASP++LYF+RVG+RK+FT+T+S KV        KG  YSFGWFAWSDGIH
Sbjct: 721 SWAPPGVAVRASPSVLYFDRVGQRKRFTVTVSGKV--------KGNGYSFGWFAWSDGIH 774

Query: 781 YVRSPIAVSST 792
           YVRSPIA+SST
Sbjct: 781 YVRSPIAISST 774

BLAST of CsaV3_6G051240 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 2.2e-252
Identity = 449/788 (56.98%), Postives = 563/788 (71.45%), Query Frame = 0

Query: 23  LFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHHSYLMYVKESEEDAKSCLLYNYK 82
           LF+    +S   +K+ YIVYFGEH G+K+  EI+E HHSYL  VKESEEDA++ LLY+YK
Sbjct: 11  LFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYK 70

Query: 83  HSINAFAAILTPQQASKLSDLDEVVSVIES--KKYRMETTRSWEFSGVEEDKPTIN---- 142
           HSIN FAA LTP QASKL  L EVVSV +S  +KY   TTRSWEF G+EE++   +    
Sbjct: 71  HSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRR 130

Query: 143 ------------DLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGP 202
                       + + +A +G  +++G+LDSGVWP+SKSF+DKGMGP+PKSWKGICQTG 
Sbjct: 131 KNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGV 190

Query: 203 AFQSAHCNRKIIGARYYLKGYEHHFGRLNKTA--DYRSPCDKDGHGSHTASIAGGRRVYN 262
           AF S+HCNRKIIGARYY+KGYE ++G  N TA  D+ SP D DGHGSHTAS A GRRV  
Sbjct: 191 AFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLG 250

Query: 263 VSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDV 322
            SA GG A G+ASGGAP ARLAIYK CWA PN  K  GN+C + DMLAA+DDAIADGV V
Sbjct: 251 ASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHV 310

Query: 323 LSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGAST 382
           +S+SIG +EP+ +T DG+A+GALHAVK++IVV+ SAGN GP P  LSN+APWIITVGAST
Sbjct: 311 ISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGAST 370

Query: 383 VDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLS 442
           +DR F   ++LGNG  IK  S+   K++  K  PLVYA +++ P    N++  C+  SL 
Sbjct: 371 LDRAFVGGLVLGNGYTIKTDSITAFKMD--KFAPLVYASNVVVPGIALNETSQCLPNSLK 430

Query: 443 HEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSY 502
            E   GK+VLC RG G SR    +EV+R+GGAGMILGN+ A G    +D HFVP   V+ 
Sbjct: 431 PELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTP 490

Query: 503 EDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGV 562
              + IL+YIK+ KNP A I P  T+Y  + AP+M  FSSRGPN +DP+ LKPDITAPG+
Sbjct: 491 TVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGL 550

Query: 563 DILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRS 622
            ILAAWS  DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS AAIRS
Sbjct: 551 YILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRS 610

Query: 623 ALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYL 682
           ALMTT+   N   +PI D + L   PA PF+ GSGHFRP+KAADPGLVYD++Y  YL Y 
Sbjct: 611 ALMTTAWMTNDKKKPIQDTTGL---PANPFALGSGHFRPTKAADPGLVYDASYRAYLLYG 670

Query: 683 CGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVG-GGGKNVYFFKS 742
           C + + +IDP+FKCP +    ++ NYPSIAVP L+  V +KRTVTNVG G   + Y F  
Sbjct: 671 CSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSV 730

Query: 743 EAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHY 790
           + P G++V A PNIL FNR+G++++F I I    N    +++KG+ Y FGWF+W+D +H 
Sbjct: 731 KPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQ-YQFGWFSWTDKVHV 789

BLAST of CsaV3_6G051240 vs. ExPASy Swiss-Prot
Match: F4KEL0 (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 785.8 bits (2028), Expect = 4.6e-226
Identity = 421/808 (52.10%), Postives = 543/808 (67.20%), Query Frame = 0

Query: 11  RLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHHSYLMYVKESE 70
           R+  + + L + LF+    +S   +K+ YIVYFGEH G+K+  EI+  HHSYL  VKE+E
Sbjct: 3   RIFGIFIFLSLLLFLVPLLASCTKEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKETE 62

Query: 71  EDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIES--KKYRMETTRSWEFSGV 130
           EDA S LLY    SIN FAA LTP QAS+L +L EVVSV +S  +KY++ TTRSWEF G+
Sbjct: 63  EDATSSLLYRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGL 122

Query: 131 EED--------------KPTIND-------LVSRANYGKDVVIGMLDSGVWPKSKSFSDK 190
           +E+              K  +ND        +  A +G  V++G++DSGVWP+S+SF DK
Sbjct: 123 KEEEGEDYRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDK 182

Query: 191 GMGPIPKSWKGICQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTA--DYRSPCDKD 250
           GMGPIP+SWKGICQTG AF S+HCNRKIIGARYY +GYE ++G  N  A  D+ SP D D
Sbjct: 183 GMGPIPESWKGICQTGVAFNSSHCNRKIIGARYYARGYERYYGPFNAEANKDFLSPRDAD 242

Query: 251 GHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFD 310
           GHGSHTAS A GRRV  VSA GG+A GTASGGA  ARLA+YK CWA+PN+ K   N CFD
Sbjct: 243 GHGSHTASTAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFD 302

Query: 311 TDMLAAMDDAIADGVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTP 370
            DMLAA DDAIADGV+V+S+SIG  EP+ Y +DG+AIGALHAVK+DIVV+ SAGN GP  
Sbjct: 303 EDMLAAFDDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPAR 362

Query: 371 SALSNVAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMN 430
             LSN APWIITVGAS++DR F   + LG+G   +  S+   K++     PLVYA D++ 
Sbjct: 363 ETLSNPAPWIITVGASSLDRFFVGRLELGDGYVFESDSLTTLKMD--NYAPLVYAPDVVV 422

Query: 431 PHAPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAG-SLEVQRSGGAGMILGNVPAV 490
           P   RN + LC+  +LS +  +GK+VLC RG G     G  LEV+R+GG GMIL N    
Sbjct: 423 PGVSRNDAMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRD 482

Query: 491 GRRPHADPHFVPATAVSYEDANIILKYIKSRKNPTATIVPPVTI-YGSRPAPAMANFSSR 550
                 + HFVP   V     + IL YI +   P A I P  T+ Y ++P  ++  FSSR
Sbjct: 483 NDAFDVESHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPFSSR 542

Query: 551 GPNPIDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSA 610
            PN +D + LKPDI APG++ILAAWS  DS +K    +D R++ YNL SGTSMSCPHV+ 
Sbjct: 543 APNWVDANILKPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAG 602

Query: 611 AAALLRAIHPTWSQAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSK 670
           A ALL+++HPTWS AAIRSALMTT++  N+  +PI D    D SPA PF+ GS HFRP+K
Sbjct: 603 AIALLKSMHPTWSSAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPTK 662

Query: 671 AADPGLVYDSNYTDYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIK 730
           AA PGLVYD++Y  YL Y C + + ++DP+FKCP R    ++LNYPSI++P L   V + 
Sbjct: 663 AASPGLVYDASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVT 722

Query: 731 RTVTNVG--GGGKNVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRS 790
           RTVT VG  G   +VY F ++ P GV V A PN+L F+++G++K+F I  + +       
Sbjct: 723 RTVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGE 782

BLAST of CsaV3_6G051240 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 4.1e-174
Identity = 359/787 (45.62%), Postives = 474/787 (60.23%), Query Frame = 0

Query: 15  LLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHH-----GEKSIEEIKERHHSYLMYVKES 74
           LLLLLL+ +  +   +S ++   +Y+VYFG H       E +++ +KE H+ +L     S
Sbjct: 10  LLLLLLVHMSSKHILASKDS--SSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 69

Query: 75  EEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSWEFSGVE 134
            E A   + Y+Y   IN FAA L    A ++S   EVVSV  +K  ++ TTRSW+F G+E
Sbjct: 70  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 129

Query: 135 EDKPTINDLVSR-ANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 194
            +    +  + R A +G+D +I  LD+GVWP+SKSF D+G+GPIP  WKGICQ      +
Sbjct: 130 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ-NQKDAT 189

Query: 195 AHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 254
            HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V  VS F G
Sbjct: 190 FHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF-G 249

Query: 255 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 314
              GTA GG+P AR+A YKVCW         GN C+D D+LAA D AI DG DV+S+S+G
Sbjct: 250 QGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSLG 309

Query: 315 KSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFY 374
             EP ++ +D +AIG+ HA KK IVV CSAGN GP  S +SNVAPW ITVGAST+DREF 
Sbjct: 310 -GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 369

Query: 375 SPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSHEKAKG 434
           S ++LGNG   KG S++ + L   K YP++ + +    +A    + LC  GSL   K KG
Sbjct: 370 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 429

Query: 435 KIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANII 494
           KI++C RG+   R      V   GG GM+L N    G    ADPH +PAT ++ +D+  +
Sbjct: 430 KILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAV 489

Query: 495 LKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVDILAAW 554
            +YI   K P A I P  T  G +PAP MA+FSS+GP+ + P  LKPDITAPGV ++AA+
Sbjct: 490 SRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAY 549

Query: 555 SEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTS 614
           +   SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +P+WS AAIRSA+MTT+
Sbjct: 550 TGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 609

Query: 615 TTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN 674
           T  +    PI + +   N  ATPFSFG+GH +P+ A +PGLVYD    DYL++LC L  N
Sbjct: 610 TIMDDIPGPIQNAT---NMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 669

Query: 675 SIDPS------FKCPPRALHPHDLNYPSIAVPQL-RNVVRIKRTVTNVGGGGKNVYFFKS 734
           +   S      F C    +   +LNYPSI VP L  + V + RTV NV  G  ++Y  K 
Sbjct: 670 ASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV--GRPSMYTVKV 729

Query: 735 EAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHY 789
             P+GV V+  P  L F +VGE+K F + + +   N        + Y FG   WSD  H 
Sbjct: 730 NNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN------VAKGYVFGELVWSDKKHR 770

BLAST of CsaV3_6G051240 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 4.8e-167
Identity = 347/785 (44.20%), Postives = 457/785 (58.22%), Query Frame = 0

Query: 17  LLLLIGLFIQQAASSSNNQKKAYIVYFGEHH-----GEKSIEEIKERHHSYLMYVKESEE 76
           LLLL+ LF   A +     KK+YIVY G H          ++ +   H ++L     S E
Sbjct: 24  LLLLVTLFFSPAFA----LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHE 83

Query: 77  DAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSWEFSGVEED 136
           +AK  + Y+YK  IN FAAIL   +A++++   +VVSV  +K  ++ TT SW F  + ++
Sbjct: 84  NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 143

Query: 137 KPT-INDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQSAH 196
                + L ++A YG+D +I  LD+GVWP+SKSFSD+G G +P  WKG C      +   
Sbjct: 144 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVP 203

Query: 197 CNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGGVA 256
           CNRK+IGARY+ KGY  + G L   A Y +  D DGHGSHT S A G  V   + F G+ 
Sbjct: 204 CNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIG 263

Query: 257 WGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIGKS 316
            GTASGG+P AR+A YKVCW   +     G  CFD D+LAA++ AI DGVDVLS S+G  
Sbjct: 264 NGTASGGSPKARVAAYKVCWPPVD-----GAECFDADILAAIEAAIEDGVDVLSASVG-G 323

Query: 317 EPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 376
           +  +Y  DG+AIG+ HAVK  + V CSAGN GP    +SNVAPW+ITVGAS++DREF + 
Sbjct: 324 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 383

Query: 377 VILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKI 436
           V L NG   KG S++   L  +KMY L+ A D    +     + LC  GSL  +K KGKI
Sbjct: 384 VELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 443

Query: 437 VLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANIILK 496
           ++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y+D   +  
Sbjct: 444 LVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 503

Query: 497 YIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVDILAAWSE 556
           Y+ S K+P   I  P     ++PAP MA+FSSRGPN I P  LKPDITAPGV+I+AA++E
Sbjct: 504 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 563

Query: 557 QDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTSTT 616
              PT L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+MTTS T
Sbjct: 564 ATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRT 623

Query: 617 NNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMNSI 676
            N   +P+ D+S      A PFS+GSGH +P+KAA PGLVYD    DYL +LC +  N+ 
Sbjct: 624 RNNRRKPMVDESF---KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNT 683

Query: 677 -------DPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFKSEA 736
                  DP + C   A +  D NYPSI VP L   + + R + NVG      Y  +   
Sbjct: 684 VVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNVGPPA--TYNARFRE 743

Query: 737 PRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHYVR 789
           P GV VS  P  L FN+ GE K F +T+                Y FG   W+D  HYVR
Sbjct: 744 PLGVRVSVEPKQLTFNKTGEVKIFQMTL-------RPLPVTPSGYVFGELTWTDSHHYVR 774

BLAST of CsaV3_6G051240 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.1e-166
Identity = 355/796 (44.60%), Postives = 474/796 (59.55%), Query Frame = 0

Query: 4   SSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHHSYL 63
           SSFL      LLL L    +      SSS++ +  YIV+  +     S +     + S L
Sbjct: 3   SSFLSSTAFFLLLCLGFCHV------SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSL 62

Query: 64  MYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSW 123
             + +S E     LLY Y+++I+ F+  LT ++A  L     V+SV+   +Y + TTR+ 
Sbjct: 63  RSISDSAE-----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTP 122

Query: 124 EFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTG 183
            F G++E      DL   A    DVV+G+LD+GVWP+SKS+SD+G GPIP SWKG C+ G
Sbjct: 123 LFLGLDEHTA---DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAG 182

Query: 184 PAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNV 243
             F ++ CNRK+IGAR++ +GYE   G ++++ + RSP D DGHG+HT+S A G  V   
Sbjct: 183 TNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGA 242

Query: 244 SAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVL 303
           S   G A GTA G AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VL
Sbjct: 243 SLL-GYASGTARGMAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVL 302

Query: 304 SLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 363
           S+S+G     +Y  DG+AIGA  A+++ I+VSCSAGN GP+ S+LSNVAPWI TVGA T+
Sbjct: 303 SMSLGGGMS-DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTL 362

Query: 364 DREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSH 423
           DR+F +  ILGNG    G+S+   +    K+ P +YAG+  N         LC+ G+L  
Sbjct: 363 DRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIP 422

Query: 424 EKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYE 483
           EK KGKIV+C RG       G + V+ +GG GMIL N  A G    AD H +PAT V  +
Sbjct: 423 EKVKGKIVMCDRGINARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEK 482

Query: 484 DANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVD 543
             +II  Y+ +  NPTA+I    T+ G +P+P +A FSSRGPN I P+ LKPD+ APGV+
Sbjct: 483 AGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVN 542

Query: 544 ILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSA 603
           ILAAW+    PT L    D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSA
Sbjct: 543 ILAAWTGAAGPTGLAS--DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSA 602

Query: 604 LMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLC 663
           LMTT+    K G+P+ D +T    P+TPF  G+GH  P+ A +PGL+YD    DYL +LC
Sbjct: 603 LMTTAYKTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLC 662

Query: 664 GLKMNSID------PSFKC-PPRALHPHDLNYPSIAV-PQLRNVVRIKRTVTNVGGGGKN 723
            L   S         ++ C P ++    DLNYPS AV        +  RTVT+VGG G  
Sbjct: 663 ALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTY 722

Query: 724 VYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAW 783
                SE   GV +S  P +L F    E+K +T+T +        SSK     SFG   W
Sbjct: 723 SVKVTSET-TGVKISVEPAVLNFKEANEKKSYTVTFTVD------SSKPSGSNSFGSIEW 757

Query: 784 SDGIHYVRSPIAVSST 792
           SDG H V SP+A+S T
Sbjct: 783 SDGKHVVGSPVAISWT 757

BLAST of CsaV3_6G051240 vs. ExPASy TrEMBL
Match: A0A0A0KHZ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1)

HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 791/791 (100.00%), Postives = 791/791 (100.00%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

Query: 781 YVRSPIAVSST 792
           YVRSPIAVSST
Sbjct: 781 YVRSPIAVSST 791

BLAST of CsaV3_6G051240 vs. ExPASy TrEMBL
Match: A0A1S3B024 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=LOC103484415 PE=3 SV=1)

HSP 1 Score: 1542.7 bits (3993), Expect = 0.0e+00
Identity = 764/793 (96.34%), Postives = 777/793 (97.98%), Query Frame = 0

Query: 1   MEKSSFLCHCR-LLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERH 60
           MEKSSFLCHC  LLLLLLLLLIG FIQQAASSSNNQKKAYIVYFGEHHGEKS+EEIKERH
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMET 120
           HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRMET
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
           TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWP SKSFSDKGMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRR 240
           CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
           VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360
           VDVLSLSIGKSEPYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420
           ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SLSHEKAKGK VLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA
Sbjct: 421 SLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540
           VSYEDAN+ILKYIKSR NPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA
Sbjct: 481 VSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600
           PGVDILAAWSEQDSPTKL KYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
           IRSALMTTSTTNNK GQPITDDST DNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL
Sbjct: 601 IRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660

Query: 661 HYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFF 720
           HYLCGLKMNSIDPSF CPPR LHPHDLNYPSIAVPQLR+VVRIKRTVTNVGGGGK+VYFF
Sbjct: 661 HYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFF 720

Query: 721 KSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVN--NNNRSSKKGEDYSFGWFAWSD 780
           KSEAP GVAVSASPNILYFNRVGERKKFTITISRKVN  +++R SKKGE+YSFGWFAWSD
Sbjct: 721 KSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAWSD 780

Query: 781 GIHYVRSPIAVSS 791
            IHYVRSPIAVS+
Sbjct: 781 EIHYVRSPIAVST 793

BLAST of CsaV3_6G051240 vs. ExPASy TrEMBL
Match: A0A6J1EMK8 (subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE=3 SV=1)

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 661/791 (83.57%), Postives = 718/791 (90.77%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           ME SS  C    LLLLLLLL         SS   QK+AYIVYFGEH G+KS EEI+ERHH
Sbjct: 1   MEISSLFC----LLLLLLLL-------PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLM VK++E DAKS LLY+YKH+IN FAA+LT QQASKLS+L+EVVSVIESKKY M TT
Sbjct: 61  SYLMSVKDTENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKP ++DLVS+A  GKDVVIGMLDSGVWPKSKSFSDKGMGP+PKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAF+S+HCNRKIIGARYY+KGYEHHFGRLN+T D+RSPCD DGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFESSHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGV
Sbjct: 241 YNVSAFGGVARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGN GPTPS LSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL R KMYPLVYA DI  PH PRN+SG C+AGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSH+KAKGKIVLC+RG+GISR+AGSLEV+RSGGAGMILG VPAVG R HADPHFVPATAV
Sbjct: 421 LSHKKAKGKIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SY+DAN ILKYIKS +NPTATIVPP+TIYGSRPAPAMANF+SRGP+ +DPHFLKPDITAP
Sbjct: 481 SYDDANRILKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSE+DSPTKLPK  DPRIV+YN+YSGTSMSCPHVSAAAALLRAIHPTWSQAAI
Sbjct: 541 GVDILAAWSEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTT+TT NKYG PITDDST DNSPATPFSFGSGHFRP+KAADPGLVYD+NYTDYLH
Sbjct: 601 RSALMTTATTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSID SFKCPPRALHPHDLNYPSIA+P+L+  VRIKRTVTNVGGGGK+VYFF+
Sbjct: 661 YLCGLKMNSIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFE 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           S AP GVAV ASP++LYF+RVG+RK+FTIT+S KV        KG  YSFGWFAWSDG+H
Sbjct: 721 SWAPPGVAVRASPSVLYFDRVGQRKRFTITVSGKV--------KGNGYSFGWFAWSDGVH 772

Query: 781 YVRSPIAVSST 792
           YVRSPIA+SST
Sbjct: 781 YVRSPIAISST 772

BLAST of CsaV3_6G051240 vs. ExPASy TrEMBL
Match: A0A6J1KU93 (subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3 SV=1)

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 655/791 (82.81%), Postives = 713/791 (90.14%), Query Frame = 0

Query: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60
           ME S+  C     LLLLLLL         SS   QK+AYIVYFGEH G+KS EEI+ERHH
Sbjct: 1   METSTLFC-----LLLLLLL-------PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHH 60

Query: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120
           SYLM VK++E DAKS LLY+YKH+IN FAA+LT QQASKLS+L+EVVSVIESK Y M TT
Sbjct: 61  SYLMSVKDTENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTT 120

Query: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGV EDKP ++DLVS+A  GKDVVIGMLDSGVWPKSKSFSD GMGP+PKSWKGIC
Sbjct: 121 RSWEFSGVVEDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGP F+S HCNRKIIGARYY+KGYEHHFGRLN+T D+RSPCD DGHGSHTASIAGGRRV
Sbjct: 181 QTGPGFESPHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGV
Sbjct: 241 YNVSAFGGVARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGV 300

Query: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGN GPTPS LSNVAPWIITVGA
Sbjct: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL R KMYPLVYA DI  PH P+N+SG C+AGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPQNESGSCIAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSH+KAKGKIVLC+RG+GISR+AGSLEV+RSGGAGMILG VPAVG R HADPHFVPATAV
Sbjct: 421 LSHKKAKGKIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAV 480

Query: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540
           SY+DAN ILKYIKS +NPTATIVPP+TIYGSRPAPAMANF+SRGP+ +DPHFLKPDITAP
Sbjct: 481 SYDDANRILKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600
           GVDILAAWSE+DSPT LPK+LDPRIV+YN+YSGTSMSCPHVSAAAALLRAIHPTWSQAAI
Sbjct: 541 GVDILAAWSEKDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTTSTT NKYG PITDDST DNSPATPFSFGSGHFRP+KAADPGLVYD+NYTDYLH
Sbjct: 601 RSALMTTSTTTNKYGHPITDDSTSDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLH 660

Query: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720
           YLCGLKMNSID SFKCPPRALHPHDLNYPSIA+P+L+  VRIKRTVTNVGGGGK+VYFF+
Sbjct: 661 YLCGLKMNSIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFE 720

Query: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780
           S AP GVAV ASP++LYF+RVG+RK+FT+T+S KV        KG  YSFGWFAWSDGIH
Sbjct: 721 SWAPPGVAVRASPSVLYFDRVGQRKRFTVTVSGKV--------KGSGYSFGWFAWSDGIH 771

Query: 781 YVRSPIAVSST 792
           YVRSPIA+SST
Sbjct: 781 YVRSPIAISST 771

BLAST of CsaV3_6G051240 vs. ExPASy TrEMBL
Match: A0A5A7UEJ8 (Subtilisin-like protease SBT5.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001930 PE=3 SV=1)

HSP 1 Score: 1283.9 bits (3321), Expect = 0.0e+00
Identity = 647/730 (88.63%), Postives = 663/730 (90.82%), Query Frame = 0

Query: 64  MYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSW 123
           MYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRMETTRSW
Sbjct: 1   MYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMETTRSW 60

Query: 124 EFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTG 183
           EFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWP SKSFSDKGMGP+PKSWKGICQTG
Sbjct: 61  EFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGICQTG 120

Query: 184 PAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNV 243
           PAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRRVYNV
Sbjct: 121 PAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNV 180

Query: 244 SAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVL 303
           SAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVL
Sbjct: 181 SAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVL 240

Query: 304 SLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 363
           SLSIGKSEPYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV
Sbjct: 241 SLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 300

Query: 364 DREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSH 423
           DREFYSPVILGNGLKIK   +    L    +   + + +  N    R    LCVAGSLSH
Sbjct: 301 DREFYSPVILGNGLKIK--VILSYVLNSFFLKSRILSFNCRNVFCGR----LCVAGSLSH 360

Query: 424 EKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYE 483
           EKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYE
Sbjct: 361 EKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYE 420

Query: 484 DANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVD 543
           DAN+ILKYIKSR NPTATIVPP                             PDITAPGVD
Sbjct: 421 DANVILKYIKSRTNPTATIVPP-----------------------------PDITAPGVD 480

Query: 544 ILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSA 603
           ILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSA
Sbjct: 481 ILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSA 540

Query: 604 LMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLC 663
           LMTTSTTNNK GQPITDDST DNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLC
Sbjct: 541 LMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLC 600

Query: 664 GLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFKSEA 723
           GLKMNSIDPSF CPPR LHPHDLNYPSIAVPQLR+VVRIKRTVTNVGGGGK+VYFFKSEA
Sbjct: 601 GLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFFKSEA 660

Query: 724 PRGVAVSASPNILYFNRVGERKKFTITISRKVNNNN---RSSKKGEDYSFGWFAWSDGIH 783
           P GVAVSASPNILYFNRVGERKKFTITISRKVNN++   R SKKGE+YSFGWF WSDGIH
Sbjct: 661 PPGVAVSASPNILYFNRVGERKKFTITISRKVNNSSSSRRRSKKGEEYSFGWFGWSDGIH 695

Query: 784 YVRSPIAVSS 791
           YVRSPIAVS+
Sbjct: 721 YVRSPIAVST 695

BLAST of CsaV3_6G051240 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 873.2 bits (2255), Expect = 1.5e-253
Identity = 449/788 (56.98%), Postives = 563/788 (71.45%), Query Frame = 0

Query: 23  LFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHHSYLMYVKESEEDAKSCLLYNYK 82
           LF+    +S   +K+ YIVYFGEH G+K+  EI+E HHSYL  VKESEEDA++ LLY+YK
Sbjct: 11  LFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYK 70

Query: 83  HSINAFAAILTPQQASKLSDLDEVVSVIES--KKYRMETTRSWEFSGVEEDKPTIN---- 142
           HSIN FAA LTP QASKL  L EVVSV +S  +KY   TTRSWEF G+EE++   +    
Sbjct: 71  HSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRR 130

Query: 143 ------------DLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGP 202
                       + + +A +G  +++G+LDSGVWP+SKSF+DKGMGP+PKSWKGICQTG 
Sbjct: 131 KNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGV 190

Query: 203 AFQSAHCNRKIIGARYYLKGYEHHFGRLNKTA--DYRSPCDKDGHGSHTASIAGGRRVYN 262
           AF S+HCNRKIIGARYY+KGYE ++G  N TA  D+ SP D DGHGSHTAS A GRRV  
Sbjct: 191 AFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLG 250

Query: 263 VSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDV 322
            SA GG A G+ASGGAP ARLAIYK CWA PN  K  GN+C + DMLAA+DDAIADGV V
Sbjct: 251 ASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHV 310

Query: 323 LSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGAST 382
           +S+SIG +EP+ +T DG+A+GALHAVK++IVV+ SAGN GP P  LSN+APWIITVGAST
Sbjct: 311 ISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGAST 370

Query: 383 VDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLS 442
           +DR F   ++LGNG  IK  S+   K++  K  PLVYA +++ P    N++  C+  SL 
Sbjct: 371 LDRAFVGGLVLGNGYTIKTDSITAFKMD--KFAPLVYASNVVVPGIALNETSQCLPNSLK 430

Query: 443 HEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSY 502
            E   GK+VLC RG G SR    +EV+R+GGAGMILGN+ A G    +D HFVP   V+ 
Sbjct: 431 PELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTP 490

Query: 503 EDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGV 562
              + IL+YIK+ KNP A I P  T+Y  + AP+M  FSSRGPN +DP+ LKPDITAPG+
Sbjct: 491 TVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGL 550

Query: 563 DILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRS 622
            ILAAWS  DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS AAIRS
Sbjct: 551 YILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRS 610

Query: 623 ALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYL 682
           ALMTT+   N   +PI D + L   PA PF+ GSGHFRP+KAADPGLVYD++Y  YL Y 
Sbjct: 611 ALMTTAWMTNDKKKPIQDTTGL---PANPFALGSGHFRPTKAADPGLVYDASYRAYLLYG 670

Query: 683 CGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVG-GGGKNVYFFKS 742
           C + + +IDP+FKCP +    ++ NYPSIAVP L+  V +KRTVTNVG G   + Y F  
Sbjct: 671 CSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSV 730

Query: 743 EAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHY 790
           + P G++V A PNIL FNR+G++++F I I    N    +++KG+ Y FGWF+W+D +H 
Sbjct: 731 KPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQ-YQFGWFSWTDKVHV 789

BLAST of CsaV3_6G051240 vs. TAIR 10
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 688.7 bits (1776), Expect = 5.4e-198
Identity = 376/735 (51.16%), Postives = 485/735 (65.99%), Query Frame = 0

Query: 84  SINAFAAILTPQQASKLSDLDEVVSVIES--KKYRMETTRSWEFSGVEED---------- 143
           SIN FAA LTP QAS+L +L EVVSV +S  +KY++ TTRSWEF G++E+          
Sbjct: 37  SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGEDYRSDGD 96

Query: 144 ----KPTIND-------LVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 203
               K  +ND        +  A +G  V++G++DSGVWP+S+SF DKGMGPIP+SWKGIC
Sbjct: 97  APRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPESWKGIC 156

Query: 204 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTA--DYRSPCDKDGHGSHTASIAGGR 263
           QTG AF S+HCN      RYY +GYE ++G  N  A  D+ SP D DGHGSHTAS A GR
Sbjct: 157 QTGVAFNSSHCN------RYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTASTAVGR 216

Query: 264 RVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIAD 323
           RV  VSA GG+A GTASGGA  ARLA+YK CWA+PN+ K   N CFD DMLAA DDAIAD
Sbjct: 217 RVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAFDDAIAD 276

Query: 324 GVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITV 383
           GV+V+S+SIG  EP+ Y +DG+AIGALHAVK+DIVV+ SAGN GP    LSN APWIITV
Sbjct: 277 GVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPAPWIITV 336

Query: 384 GASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVA 443
           GAS++DR F   + LG+G   +  S+   K++     PLVYA D++ P   RN + LC+ 
Sbjct: 337 GASSLDRFFVGRLELGDGYVFESDSLTTLKMD--NYAPLVYAPDVVVPGVSRNDAMLCLP 396

Query: 444 GSLSHEKAKGKIVLCFRGEGISRFAG-SLEVQRSGGAGMILGNVPAVGRRPHADPHFVPA 503
            +LS +  +GK+VLC RG G     G  LEV+R+GG GMIL N          + HFVP 
Sbjct: 397 NALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVESHFVPT 456

Query: 504 TAVSYEDANIILKYIKSRKNPTATIVPPVTI-YGSRPAPAMANFSSRGPNPIDPHFLKPD 563
             V     + IL YI +   P A I P  T+ Y ++P  ++  +    P P    FL PD
Sbjct: 457 ALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPYK---PAPFMTSFL-PD 516

Query: 564 ITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWS 623
           I APG++ILAAWS  DS +K    +D R++ YNL SGTSMSCPHV+ A ALL+++HPTWS
Sbjct: 517 IIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIALLKSMHPTWS 576

Query: 624 QAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYT 683
            AAIRSALMTT++  N+  +PI D    D SPA PF+ GS HFRP+KAA PGLVYD++Y 
Sbjct: 577 SAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPTKAASPGLVYDASYQ 636

Query: 684 DYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVG--GGGK 743
            YL Y C + + ++DP+FKCP R    ++LNYPSI++P L   V + RTVT VG  G   
Sbjct: 637 SYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTVTCVGRTGNST 696

Query: 744 NVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFA 790
           +VY F ++ P GV V A PN+L F+++G++K+F I  + +       +++ + Y FGWF+
Sbjct: 697 SVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEARR-DRYRFGWFS 752

BLAST of CsaV3_6G051240 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 613.2 bits (1580), Expect = 2.9e-175
Identity = 359/787 (45.62%), Postives = 474/787 (60.23%), Query Frame = 0

Query: 15  LLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHH-----GEKSIEEIKERHHSYLMYVKES 74
           LLLLLL+ +  +   +S ++   +Y+VYFG H       E +++ +KE H+ +L     S
Sbjct: 10  LLLLLLVHMSSKHILASKDS--SSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 69

Query: 75  EEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSWEFSGVE 134
            E A   + Y+Y   IN FAA L    A ++S   EVVSV  +K  ++ TTRSW+F G+E
Sbjct: 70  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 129

Query: 135 EDKPTINDLVSR-ANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 194
            +    +  + R A +G+D +I  LD+GVWP+SKSF D+G+GPIP  WKGICQ      +
Sbjct: 130 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ-NQKDAT 189

Query: 195 AHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 254
            HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V  VS F G
Sbjct: 190 FHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF-G 249

Query: 255 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 314
              GTA GG+P AR+A YKVCW         GN C+D D+LAA D AI DG DV+S+S+G
Sbjct: 250 QGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSLG 309

Query: 315 KSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFY 374
             EP ++ +D +AIG+ HA KK IVV CSAGN GP  S +SNVAPW ITVGAST+DREF 
Sbjct: 310 -GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 369

Query: 375 SPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSHEKAKG 434
           S ++LGNG   KG S++ + L   K YP++ + +    +A    + LC  GSL   K KG
Sbjct: 370 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 429

Query: 435 KIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANII 494
           KI++C RG+   R      V   GG GM+L N    G    ADPH +PAT ++ +D+  +
Sbjct: 430 KILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAV 489

Query: 495 LKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVDILAAW 554
            +YI   K P A I P  T  G +PAP MA+FSS+GP+ + P  LKPDITAPGV ++AA+
Sbjct: 490 SRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAY 549

Query: 555 SEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTS 614
           +   SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +P+WS AAIRSA+MTT+
Sbjct: 550 TGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 609

Query: 615 TTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN 674
           T  +    PI + +   N  ATPFSFG+GH +P+ A +PGLVYD    DYL++LC L  N
Sbjct: 610 TIMDDIPGPIQNAT---NMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 669

Query: 675 SIDPS------FKCPPRALHPHDLNYPSIAVPQL-RNVVRIKRTVTNVGGGGKNVYFFKS 734
           +   S      F C    +   +LNYPSI VP L  + V + RTV NV  G  ++Y  K 
Sbjct: 670 ASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV--GRPSMYTVKV 729

Query: 735 EAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHY 789
             P+GV V+  P  L F +VGE+K F + + +   N        + Y FG   WSD  H 
Sbjct: 730 NNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN------VAKGYVFGELVWSDKKHR 770

BLAST of CsaV3_6G051240 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 3.4e-168
Identity = 347/785 (44.20%), Postives = 457/785 (58.22%), Query Frame = 0

Query: 17  LLLLIGLFIQQAASSSNNQKKAYIVYFGEHH-----GEKSIEEIKERHHSYLMYVKESEE 76
           LLLL+ LF   A +     KK+YIVY G H          ++ +   H ++L     S E
Sbjct: 24  LLLLVTLFFSPAFA----LKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHE 83

Query: 77  DAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSWEFSGVEED 136
           +AK  + Y+YK  IN FAAIL   +A++++   +VVSV  +K  ++ TT SW F  + ++
Sbjct: 84  NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 143

Query: 137 KPT-INDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQSAH 196
                + L ++A YG+D +I  LD+GVWP+SKSFSD+G G +P  WKG C      +   
Sbjct: 144 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVP 203

Query: 197 CNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGGVA 256
           CNRK+IGARY+ KGY  + G L   A Y +  D DGHGSHT S A G  V   + F G+ 
Sbjct: 204 CNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIG 263

Query: 257 WGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIGKS 316
            GTASGG+P AR+A YKVCW   +     G  CFD D+LAA++ AI DGVDVLS S+G  
Sbjct: 264 NGTASGGSPKARVAAYKVCWPPVD-----GAECFDADILAAIEAAIEDGVDVLSASVG-G 323

Query: 317 EPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTVDREFYSP 376
           +  +Y  DG+AIG+ HAVK  + V CSAGN GP    +SNVAPW+ITVGAS++DREF + 
Sbjct: 324 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 383

Query: 377 VILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKI 436
           V L NG   KG S++   L  +KMY L+ A D    +     + LC  GSL  +K KGKI
Sbjct: 384 VELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 443

Query: 437 VLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANIILK 496
           ++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y+D   +  
Sbjct: 444 LVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 503

Query: 497 YIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVDILAAWSE 556
           Y+ S K+P   I  P     ++PAP MA+FSSRGPN I P  LKPDITAPGV+I+AA++E
Sbjct: 504 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 563

Query: 557 QDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTSTT 616
              PT L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+MTTS T
Sbjct: 564 ATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRT 623

Query: 617 NNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMNSI 676
            N   +P+ D+S      A PFS+GSGH +P+KAA PGLVYD    DYL +LC +  N+ 
Sbjct: 624 RNNRRKPMVDESF---KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNT 683

Query: 677 -------DPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFKSEA 736
                  DP + C   A +  D NYPSI VP L   + + R + NVG      Y  +   
Sbjct: 684 VVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNVGPPA--TYNARFRE 743

Query: 737 PRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIHYVR 789
           P GV VS  P  L FN+ GE K F +T+                Y FG   W+D  HYVR
Sbjct: 744 PLGVRVSVEPKQLTFNKTGEVKIFQMTL-------RPLPVTPSGYVFGELTWTDSHHYVR 774

BLAST of CsaV3_6G051240 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 588.6 bits (1516), Expect = 7.6e-168
Identity = 355/796 (44.60%), Postives = 474/796 (59.55%), Query Frame = 0

Query: 4   SSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHHSYL 63
           SSFL      LLL L    +      SSS++ +  YIV+  +     S +     + S L
Sbjct: 3   SSFLSSTAFFLLLCLGFCHV------SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSL 62

Query: 64  MYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETTRSW 123
             + +S E     LLY Y+++I+ F+  LT ++A  L     V+SV+   +Y + TTR+ 
Sbjct: 63  RSISDSAE-----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTP 122

Query: 124 EFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTG 183
            F G++E      DL   A    DVV+G+LD+GVWP+SKS+SD+G GPIP SWKG C+ G
Sbjct: 123 LFLGLDEHTA---DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAG 182

Query: 184 PAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRVYNV 243
             F ++ CNRK+IGAR++ +GYE   G ++++ + RSP D DGHG+HT+S A G  V   
Sbjct: 183 TNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGA 242

Query: 244 SAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVL 303
           S   G A GTA G AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VL
Sbjct: 243 SLL-GYASGTARGMAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVL 302

Query: 304 SLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGASTV 363
           S+S+G     +Y  DG+AIGA  A+++ I+VSCSAGN GP+ S+LSNVAPWI TVGA T+
Sbjct: 303 SMSLGGGMS-DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTL 362

Query: 364 DREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGSLSH 423
           DR+F +  ILGNG    G+S+   +    K+ P +YAG+  N         LC+ G+L  
Sbjct: 363 DRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIP 422

Query: 424 EKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYE 483
           EK KGKIV+C RG       G + V+ +GG GMIL N  A G    AD H +PAT V  +
Sbjct: 423 EKVKGKIVMCDRGINARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEK 482

Query: 484 DANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAPGVD 543
             +II  Y+ +  NPTA+I    T+ G +P+P +A FSSRGPN I P+ LKPD+ APGV+
Sbjct: 483 AGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVN 542

Query: 544 ILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSA 603
           ILAAW+    PT L    D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSA
Sbjct: 543 ILAAWTGAAGPTGLAS--DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSA 602

Query: 604 LMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLC 663
           LMTT+    K G+P+ D +T    P+TPF  G+GH  P+ A +PGL+YD    DYL +LC
Sbjct: 603 LMTTAYKTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLC 662

Query: 664 GLKMNSID------PSFKC-PPRALHPHDLNYPSIAV-PQLRNVVRIKRTVTNVGGGGKN 723
            L   S         ++ C P ++    DLNYPS AV        +  RTVT+VGG G  
Sbjct: 663 ALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTY 722

Query: 724 VYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAW 783
                SE   GV +S  P +L F    E+K +T+T +        SSK     SFG   W
Sbjct: 723 SVKVTSET-TGVKISVEPAVLNFKEANEKKSYTVTFTVD------SSKPSGSNSFGSIEW 757

Query: 784 SDGIHYVRSPIAVSST 792
           SDG H V SP+A+S T
Sbjct: 783 SDGKHVVGSPVAISWT 757