CsaV3_6G041050 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_6G041050 vs. NCBI nr
Match: XP_011657732.1 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN48299.1 hypothetical protein Csa_004059 [Cucumis sativus])

HSP 1 Score: 924.1 bits (2387), Expect = 4.8e-265
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS
Sbjct: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG
Sbjct: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR
Sbjct: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD
Sbjct: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

BLAST of CsaV3_6G041050 vs. NCBI nr
Match: XP_008462617.1 (PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis melo] >KAA0025220.1 aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPL TFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLATFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLTMMT+
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
             VVG GGGPAII GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

BLAST of CsaV3_6G041050 vs. NCBI nr
Match: TYK07446.1 (aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 842.0 bits (2174), Expect = 2.4e-240
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPLTTFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLTTFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSV QPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVRQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLTMMT+
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
             VVG GGGPAII GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

BLAST of CsaV3_6G041050 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 770.4 bits (1988), Expect = 8.9e-219
Identity = 389/464 (83.84%), Postives = 408/464 (87.93%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSS--STTVLPLTTFPSVSFT-DPFKTINLLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSS  ST  LPLT FPS+  T DP K IN LLSASLNRAQH
Sbjct: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60

Query: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120
           LK PQ+K   SIQNVSLF RSYGAYS++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRCS
Sbjct: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120

Query: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180
            CSFP VD ATI KFVPKLSSS K++GCRNPKCAWIFGPNL SRCRNCN KSR CS SCP
Sbjct: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180

Query: 181 GYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240
           GYG+ YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240

Query: 241 LKRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYL 300
           LKRFS+CLVSRGFDDSPVSSPLVLDSGSESD+SKT+SFIYAPFRENPS SNAAFREYYYL
Sbjct: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360

Query: 361 RAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTM 420
           R K VE QSGLRPCF+I K E S EFP++VLKFKGG KLSL   NYLA+VTD GVVCLTM
Sbjct: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420

Query: 421 MTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           MTD  VVGGG GPAII GAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 460

BLAST of CsaV3_6G041050 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 754.2 bits (1946), Expect = 6.6e-214
Identity = 374/463 (80.78%), Postives = 408/463 (88.12%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTT--VLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60
           MEF PIPFL SI LLL  SSSSS+T   LPLT FPS+ FT P+K I  L+SASL RAQHL
Sbjct: 1   MEFFPIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFTHPWKNIKHLVSASLTRAQHL 60

Query: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120
           KTP+ KSNTSIQNV+LFPRSYGAYS+SLAFGTPPQ+LS +FDTGSSLVWFPCTAGYRCS 
Sbjct: 61  KTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180
           CSFP VD ATI KF+PKLSSS K++GCRN KC+WIFGPNLKS CR+C+ +SRKCSD+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTCPG 180

Query: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300
           KRFSHCLV R FDDSPVSSPLVLDS SES ESK  S IYAPFRENPS SNAAFREYYYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+S GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMM 420
           AK VEA+SGLRPCF+I K EES EFP+++LKFKGG  L+L   NYLA+VTD GVVCLTM+
Sbjct: 361 AKGVEAESGLRPCFDISK-EESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           TD   +GGGGGPAII GAFQQQNVLV+YDLAK RIGFRKQ+CT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKDRIGFRKQRCT 462

BLAST of CsaV3_6G041050 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.5e-48
Identity = 156/488 (31.97%), Postives = 233/488 (47.75%), Query Frame = 0

Query: 19  SSSSSTTVLPLTTFPSVSFTDPFKT-INLLLSASLNRAQHLKTPQSKSNTSIQNVSLFPR 78
           S SS +T L L    S+S +    + ++LL S+S   +   +    K     Q +SL   
Sbjct: 21  SVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQ--QQLSLPIS 80

Query: 79  SYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLS 138
           S   Y +SL+ G+    +S   DTGS LVWFPC   + C  C    + P+  S     LS
Sbjct: 81  SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSPPS----SLS 140

Query: 139 SSVKVVGCRNPKCAWIFG--PNLK----SRCRNCNSKSRKCSDS---CPGYGLQYGSGAT 198
           SS   V C +P C+      P+      S C     ++  C+ S   CP +   YG G+ 
Sbjct: 141 SSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL 200

Query: 199 AGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL------KRFS 258
              L S++L L +  V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS
Sbjct: 201 VAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFS 260

Query: 259 HCLVSRGFDDSPV--SSPLVL--------------DSGSESDESKTK--SFIYAPFRENP 318
           +CLVS  FD   V   SPL+L              D   + D+ K K   F++    ENP
Sbjct: 261 YCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENP 320

Query: 319 SVSNAAFREYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEA 378
                    +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + +
Sbjct: 321 K-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 380

Query: 379 IADELEKQLVK-YPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGG-GKLSLAAEN 438
           + +E + ++ + + RA  VE  SG+ PC+ +    ++ + P +VL F G    ++L   N
Sbjct: 381 VVEEFDSRVGRVHERADRVEPSSGMSPCYYL---NQTVKVPALVLHFAGNRSSVTLPRRN 440

Query: 439 YLAMVTDEG--------VVCLTMMT--DEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQR 461
           Y     D G        + CL +M   DE+ + GG G   ILG +QQQ   V YDL  +R
Sbjct: 441 YFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTG--AILGNYQQQGFEVVYDLLNRR 491

BLAST of CsaV3_6G041050 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.5e-38
Identity = 119/386 (30.83%), Postives = 179/386 (46.37%), Query Frame = 0

Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y +++A GTP  + S I DTGS L+W  C     C++C   +  P  I  F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQC---FSQPTPI--FNPQDSSS 153

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGATA-GILLSETLD 199
              + C +  C               +  S  C+++   Y   YG G+T  G + +ET  
Sbjct: 154 FSTLPCESQYCQ--------------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 200 LENKRVPDFLVGCSV----MSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
            E   VP+   GC            AG+ G G GP SLPSQ+ + +FS+C+ S G   SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKFPYK 319
            +  L   +    + S + + I++    NP+        YYY++L+ I +GG  +  P  
Sbjct: 274 STLALGSAASGVPEGSPSTTLIHSSL--NPT--------YYYITLQGITVGGDNLGIPSS 333

Query: 320 YLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFNI 379
                  G GG IIDSG+T T+L +  + A+A     Q +  P     E+ SGL  CF  
Sbjct: 334 TFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLPTVD--ESSSGLSTCFQQ 393

Query: 380 PKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEAVVGGGGGPAIIL 439
           P +  + + P++ ++F  GG L+L  +N L +   EGV+CL M +   +         I 
Sbjct: 394 PSDGSTVQVPEISMQF-DGGVLNLGEQNIL-ISPAEGVICLAMGSSSQL------GISIF 435

Query: 440 GAFQQQNVLVEYDLAKQRIGFRKQKC 461
           G  QQQ   V YDL    + F   +C
Sbjct: 454 GNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CsaV3_6G041050 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.9e-37
Identity = 114/387 (29.46%), Postives = 171/387 (44.19%), Query Frame = 0

Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y ++L+ GTP Q  S I DTGS L+W  C    +C   S P         F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPI--------FNPQGSSS 152

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
              + C +  C  +  P               CS++   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSPT--------------CSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 200 LENKRVPDFLVGCSV----MSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
             +  +P+   GC            AG+ G GRGP SLPSQ+ + +FS+C+   G   S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIG---SS 272

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKF-PY 319
             S L+L S + S        + A       + ++    +YY++L  + +G   +   P 
Sbjct: 273 TPSNLLLGSLANS--------VTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPS 332

Query: 320 KYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFN 379
            + +  + G GG IIDSG+T T+     ++++  E   Q +  P      + SG   CF 
Sbjct: 333 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLCFQ 392

Query: 380 IPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEAVVGGGGGPAII 439
            P +  + + P  V+ F  GG L L +ENY  +    G++CL M       G       I
Sbjct: 393 TPSDPSNLQIPTFVMHF-DGGDLELPSENYF-ISPSNGLICLAM-------GSSSQGMSI 434

Query: 440 LGAFQQQNVLVEYDLAKQRIGFRKQKC 461
            G  QQQN+LV YD     + F   +C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CsaV3_6G041050 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-34
Identity = 129/412 (31.31%), Postives = 176/412 (42.72%), Query Frame = 0

Query: 57  HLKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC 116
           H   P   S++ +  +S   +  G Y   L  GTP + +  + DTGS +VW  C    RC
Sbjct: 120 HAPRPGGFSSSVVSGLS---QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC 179

Query: 117 SRCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSC 176
              S P  D        P+ S +   + C +P C        +     CN++ + C    
Sbjct: 180 YSQSDPIFD--------PRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTCL--- 239

Query: 177 PGYGLQYGSGA-TAGILLSETLDLENKRVPDFLVGCSVMS---VHQPAGIAGFGRGPESL 236
             Y + YG G+ T G   +ETL     RV    +GC   +       AG+ G G+G  S 
Sbjct: 240 --YQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSF 299

Query: 237 PSQMRLK---RFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNA 296
           P Q   +   +FS+CLV R     P  S +V  + + S  +          R  P +SN 
Sbjct: 300 PGQTGHRFNQKFSYCLVDRSASSKP--SSVVFGNAAVSRIA----------RFTPLLSNP 359

Query: 297 AFREYYYLSLRRILIGGKPVKFPYKYLVP-DSTGNGGAIIDSGSTFTFLDKPIFEAIADE 356
               +YY+ L  I +GG  V      L   D  GNGG IIDSG++ T L +P + A+ D 
Sbjct: 360 KLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDA 419

Query: 357 LEKQLVKYPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVT 416
                    RA D    S    CF++    E  + P VVL F+ G  +SL A NYL  V 
Sbjct: 420 FRVGAKTLKRAPDF---SLFDTCFDLSNMNE-VKVPTVVLHFR-GADVSLPATNYLIPVD 479

Query: 417 DEGVVCLTMMTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKC 461
             G  C       A  G  GG +II G  QQQ   V YDLA  R+GF    C
Sbjct: 480 TNGKFCF------AFAGTMGGLSII-GNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of CsaV3_6G041050 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.2e-31
Identity = 114/386 (29.53%), Postives = 166/386 (43.01%), Query Frame = 0

Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y   +  GTP + +  + DTGS + W  C     C+ C +   DP     F P  SS+
Sbjct: 160 GEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP---CADC-YQQSDPV----FNPTSSST 219

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
            K + C  P+C+ +      S CR     S KC      Y + YG G+ T G L ++T+ 
Sbjct: 220 YKSLTCSAPQCSLL----ETSACR-----SNKCL-----YQVSYGDGSFTVGELATDTVT 279

Query: 200 LENK-RVPDFLVGCSVMS---VHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
             N  ++ +  +GC   +       AG+ G G G  S+ +QM+   FS+CLV R   DS 
Sbjct: 280 FGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDR---DSG 339

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKFPYK 319
            SS L  +S        T     AP   N  +       +YY+ L    +GG+ V  P  
Sbjct: 340 KSSSLDFNSVQLGGGDAT-----APLLRNKKIDT-----FYYVGLSGFSVGGEKVVLPDA 399

Query: 320 YLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFNI 379
               D++G+GG I+D G+  T L    + ++ D   K  V     K   + S    C++ 
Sbjct: 400 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNL--KKGSSSISLFDTCYDF 459

Query: 380 PKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEAVVGGGGGPAIIL 439
                + + P V   F GG  L L A+NYL  V D G  C       + +        I+
Sbjct: 460 -SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLS-------II 500

Query: 440 GAFQQQNVLVEYDLAKQRIGFRKQKC 461
           G  QQQ   + YDL+K  IG    KC
Sbjct: 520 GNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CsaV3_6G041050 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.3e-265
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS
Sbjct: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG
Sbjct: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR
Sbjct: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD
Sbjct: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

BLAST of CsaV3_6G041050 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.2e-241
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPL TFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLATFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLTMMT+
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
             VVG GGGPAII GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

BLAST of CsaV3_6G041050 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.2e-241
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPL TFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLATFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLTMMT+
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
             VVG GGGPAII GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

BLAST of CsaV3_6G041050 vs. ExPASy TrEMBL
Match: A0A5D3CAS4 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001760 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPLTTFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLTTFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSV QPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVRQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTD 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLTMMT+
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 EAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
             VVG GGGPAII GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

BLAST of CsaV3_6G041050 vs. ExPASy TrEMBL
Match: A0A6J1IMR7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.9e-212
Identity = 370/463 (79.91%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLPTSSSSS--TTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60
           MEF PI FL SI LLL  SSSSS  T  LPLT FPS+  T P+K I  L+SASL RAQHL
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHL 60

Query: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120
           KTP++KSNTSIQNV+LFPRSYGAYS+SLAFGTPPQ+LS +FDTGSSLVWFPCTAGYRCS 
Sbjct: 61  KTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180
           CSFP VD ATI KF+PKLSSS +++GCRN KC+WIFGPNLKS CR+C+ +SRKCSD+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCPG 180

Query: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300
           KRFSHCLV R FDDSPVSSPLVLDS  ES +SKT S IYAPFRENPS SNAAFREYYYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+S GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMM 420
           AK VEA+SGLRPCF+I K EES EFP+++LKFKGG  L+L   NYLA+VTD GVVCLTM+
Sbjct: 361 AKGVEAESGLRPCFDISK-EESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           TD   +GGGGGPAII GAFQQQNVLV+YDLAK+RIGFRKQ+CT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

BLAST of CsaV3_6G041050 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 475.7 bits (1223), Expect = 4.2e-134
Identity = 243/470 (51.70%), Postives = 320/470 (68.09%), Query Frame = 0

Query: 8   FLFSIFLLLPTSSSSSTTVLPLTTF--PSVSFTDPFKTINLLLSASLNRAQHLK------ 67
           F F IFL     S  S   LPL+ F     S  DP+ ++  L  +S+ RA  LK      
Sbjct: 7   FFFLIFL-----SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSIK 66

Query: 68  ------TPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAG 127
                 +  + ++ ++    L  +SYG YSVSL+FGTP Q + F+FDTGSSLVW PCT+ 
Sbjct: 67  PDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTSR 126

Query: 128 YRCSRCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCS 187
           Y CS C F  +DP  I +F+PK SSS K++GC++PKC +++GPN+  +CR C+  +R C+
Sbjct: 127 YLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNCT 186

Query: 188 DSCPGYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLP 247
             CP Y LQYG G+TAG+L++E LD  +  VPDF+VGCS++S  QPAGIAGFGRGP SLP
Sbjct: 187 VGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLP 246

Query: 248 SQMRLKRFSHCLVSRGFDDSPVSSPLVLDSGS-ESDESKTKSFIYAPFRENPSVSNAAFR 307
           SQM LKRFSHCLVSR FDD+ V++ L LD+GS  +  SKT    Y PFR+NP+VSN AF 
Sbjct: 247 SQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFL 306

Query: 308 EYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQ 367
           EYYYL+LRRI +G K VK PYKYL P + G+GG+I+DSGSTFTF+++P+FE +A+E   Q
Sbjct: 307 EYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQ 366

Query: 368 LVKYPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGV 427
           +  Y R KD+E ++GL PCFNI  + +    P+++ +FKGG KL L   NY   V +   
Sbjct: 367 MSNYTREKDLEKETGLGPCFNISGKGD-VTVPELIFEFKGGAKLELPLSNYFTFVGNTDT 426

Query: 428 VCLTMMTDEAV-VGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           VCLT+++D+ V   GG GPAIILG+FQQQN LVEYDL   R GF K+KC+
Sbjct: 427 VCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

BLAST of CsaV3_6G041050 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 199.9 bits (507), Expect = 4.4e-51
Identity = 156/471 (33.12%), Postives = 213/471 (45.22%), Query Frame = 0

Query: 17  PTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKTPQSKSNTSIQNVSLFP 76
           P+SSSSS  VL LT   SVS   P               + +K P S  +  ++ +    
Sbjct: 32  PSSSSSSFLVLTLTK-SSVSLPTP----------KSQTQERIKKPLSSVDVVMEPLR--- 91

Query: 77  RSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPC-TAGYRCSRC-SFPYVDPATISKFVP 136
                Y ++L  GTPPQ +    DTGS L W PC    + C  C      D  + S F P
Sbjct: 92  EVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSP 151

Query: 137 KLSSSVKVVGCRNPKCAWI------FGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA- 196
             SS+     C +  C  I      F P   + C         C   CP +   YG G  
Sbjct: 152 LHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCPSFAYTYGEGGL 211

Query: 197 TAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL--KRFSHCL 256
            +GIL  + L    + VP F  GC   +  +P GIAGFGRG  SLPSQ+    K FSHC 
Sbjct: 212 ISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCF 271

Query: 257 VSRGFDDSP-VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILI 316
           +   F ++P +SSPL+L + + S  + T S  + P    P   N+     YY+ L  I I
Sbjct: 272 LPFKFVNNPNISSPLILGASALS-INLTDSLQFTPMLNTPMYPNS-----YYIGLESITI 331

Query: 317 GGK--PVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDV 376
           G    P + P      DS GNGG ++DSG+T+T L +P +  +   L+   + YPRA + 
Sbjct: 332 GTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ-STITYPRATET 391

Query: 377 EAQSGLRPCFNIP---------KEEESAEFPDVVLKFKGGGKLSLAAEN--YLAMVTDEG 436
           E+++G   C+ +P         + +    FP +   F     L L   N  Y      +G
Sbjct: 392 ESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQGNSFYAMSAPSDG 451

Query: 437 --VVCLTMMTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKC 461
             V CL     E    G  GPA + G+FQQQNV V YDL K+RIGF+   C
Sbjct: 452 SVVQCLLFQNME---DGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

BLAST of CsaV3_6G041050 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 193.7 bits (491), Expect = 3.2e-49
Identity = 156/488 (31.97%), Postives = 233/488 (47.75%), Query Frame = 0

Query: 19  SSSSSTTVLPLTTFPSVSFTDPFKT-INLLLSASLNRAQHLKTPQSKSNTSIQNVSLFPR 78
           S SS +T L L    S+S +    + ++LL S+S   +   +    K     Q +SL   
Sbjct: 21  SVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQ--QQLSLPIS 80

Query: 79  SYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLS 138
           S   Y +SL+ G+    +S   DTGS LVWFPC   + C  C    + P+  S     LS
Sbjct: 81  SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSPPS----SLS 140

Query: 139 SSVKVVGCRNPKCAWIFG--PNLK----SRCRNCNSKSRKCSDS---CPGYGLQYGSGAT 198
           SS   V C +P C+      P+      S C     ++  C+ S   CP +   YG G+ 
Sbjct: 141 SSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL 200

Query: 199 AGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL------KRFS 258
              L S++L L +  V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS
Sbjct: 201 VAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFS 260

Query: 259 HCLVSRGFDDSPV--SSPLVL--------------DSGSESDESKTK--SFIYAPFRENP 318
           +CLVS  FD   V   SPL+L              D   + D+ K K   F++    ENP
Sbjct: 261 YCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENP 320

Query: 319 SVSNAAFREYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEA 378
                    +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + +
Sbjct: 321 K-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 380

Query: 379 IADELEKQLVK-YPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGG-GKLSLAAEN 438
           + +E + ++ + + RA  VE  SG+ PC+ +    ++ + P +VL F G    ++L   N
Sbjct: 381 VVEEFDSRVGRVHERADRVEPSSGMSPCYYL---NQTVKVPALVLHFAGNRSSVTLPRRN 440

Query: 439 YLAMVTDEG--------VVCLTMMT--DEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQR 461
           Y     D G        + CL +M   DE+ + GG G   ILG +QQQ   V YDL  +R
Sbjct: 441 YFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTG--AILGNYQQQGFEVVYDLLNRR 491

BLAST of CsaV3_6G041050 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-40
Identity = 125/399 (31.33%), Postives = 187/399 (46.87%), Query Frame = 0

Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y + +  GTPP++ S I DTGS L W  C   Y C   +  + D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
            K + C +P+C+ I  P+   +C + N        SCP Y   YG  + T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 200 L---------ENKRVPDFLVGCSVMS---VHQPAGIAGFGRGPESLPSQMRL---KRFSH 259
           +            +V + + GC   +       +G+ G GRGP S  SQ++      FS+
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 260 CLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRIL 319
           CLV R   ++ VSS L+   G + D     +  +  F      S   F   YY+ ++ IL
Sbjct: 338 CLVDRN-SNTNVSSKLIF--GEDKDLLNHTNLNFTSFVNGKENSVETF---YYIQIKSIL 397

Query: 320 IGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADEL-EKQLVKYPRAKDV 379
           +GGK +  P +     S G+GG IIDSG+T ++  +P +E I ++  EK    YP  +D 
Sbjct: 398 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF 457

Query: 380 EAQSGLRPCFNIPK-EEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDE 439
                L PCFN+   EE +   P++ + F  G   +  AEN    ++ E +VCL      
Sbjct: 458 PV---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLS-EDLVCL------ 517

Query: 440 AVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKC 461
           A++G       I+G +QQQN  + YD  + R+GF   KC
Sbjct: 518 AILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of CsaV3_6G041050 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.8e-39
Identity = 123/388 (31.70%), Postives = 178/388 (45.88%), Query Frame = 0

Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G + + L+ G P    S I DTGS L+W  C     C+ C   +  P  I  F P+ SSS
Sbjct: 105 GEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKP---CTEC---FDQPTPI--FDPEKSSS 164

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGS-GATAGILLSETLD 199
              VGC +  C      N   R  NCN     C      Y   YG   +T G+L +ET  
Sbjct: 165 YSKVGCSSGLC------NALPR-SNCNEDKDACE-----YLYTYGDYSSTRGLLATETFT 224

Query: 200 LENKR-VPDFLVGCSVMS----VHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDS 259
            E++  +     GC V +      Q +G+ G GRGP SL SQ++  +FS+CL S   +DS
Sbjct: 225 FEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTS--IEDS 284

Query: 260 PVSSPLVLDSGSESDESKTKSFIYAPFRENPS-VSNAAFREYYYLSLRRILIGGKPVKFP 319
             SS L + S +    +KT + +     +  S + N     +YYL L+ I +G K +   
Sbjct: 285 EASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVE 344

Query: 320 YKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCF 379
                    G GG IIDSG+T T+L++  F+ + +E   ++       D    +GL  CF
Sbjct: 345 KSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRM---SLPVDDSGSTGLDLCF 404

Query: 380 NIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEAVVGGGGGPAI 439
            +P   ++   P ++  FK G  L L  ENY+   +  GV+CL M        G      
Sbjct: 405 KLPDAAKNIAVPKMIFHFK-GADLELPGENYMVADSSTGVLCLAM--------GSSNGMS 458

Query: 440 ILGAFQQQNVLVEYDLAKQRIGFRKQKC 461
           I G  QQQN  V +DL K+ + F   +C
Sbjct: 465 IFGNVQQQNFNVLHDLEKETVSFVPTEC 458