CsaV3_6G040000 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G040000
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionPectate lyase
Locationchr6: 23007604 .. 23011325 (+)
RNA-Seq ExpressionCsaV3_6G040000
SyntenyCsaV3_6G040000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTCAGTCCCATAGTCAAAAAGTTACCCTCTTTTCTCTTCACACTTATTTCTTTACGTTCCACCATTATCCAATTCCTAATCAAATATGTTCCATTATTTATACGGCCCAATCATCTTCTGCCACCTCATCACTCTACAAATCTCTTTTATTATATAATATATTTATATATATATACTTAGGAGTTTTATATAATTTTAAATACTATTTTCATTATTTATACTTTTTAGCTTTGGTTATCGCTCGTCCTATTTTTCGTCCTTTAAAAATGAAAATGAAAGATCAAAGTGTAAGTTTTAAAAAGTATAAGGACTAAAATAAACAAAATTTAAAGTGAGAATTATAAATTAGTATTTAATTTGAGTTTAGAAGGTTAAAATTCTCCCCCGAGTCCTTATTTGTACCAAACTTAACACCAACAAATTAAGGTATGTTTAGATTAGAAGAGGCCAAGGATGGGATCTCTCCGTCTCACTTTGTGGAACTAAAAGAAAACCCAAATGAAATTAACCCGTTTCCCTTCAAAAGTAAATTGGATACAACTTTCCCTCCTCGAGAACCAAAGATTCTCTCTCCCCCCATTCTCTCACTAACTGAATCCCAAAAGTCTCTGACAATGATTATATTAGACAGAATGAGAGCTGAGAATGCTTTTGAGGGTAACATGGAAGGAAGAAAAATGAAGAAAAACAGTTGAAAAACAGTTCTCCACTCAAAACACAACAAACGTAACACACAACTGCACAGCAGTTTTCTAAGCCTGTAGAGAGACTGAGTGAGAGAGCTTACCATTTTTGCTCTCCGAAATGCTGGCCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCCGCTTGCCGCTCCGTGCCGCTGCCACCACCTCCGCCGCTCTCAACCTCACTCTCCCTGGCCAGCATCCTAGCCCTGAATTGGTTGCTCAAGAAGTTCACAGGTTCTTCAATGTTCCATTTTTTCTTTTTTCACAAATGTTGTTTAAGAATCACTATGTTCTTGGCTTGGCTCTTACTCTAACTGTCTGCATTTTCAAACTTTTAGCTACATTGTGCATTTTCTCTACTTTATTGCCCGTTTCAAGATTTTTATGCTTATTTTGGTAGGATTTCATGTATTAGATGTACAAAATCCAAGTTTAGAATCAGGGTTTTTTTTGTTACTATGTTCTTGGTTTGTTCCTCTTTTTTCCTTGACCTTCTTTGTGGTAGCTCTGTGGGTAGCTACAGTCTTCAGCTTTAAGCTTTTCTTGACCGCATTATTAGGCCTTAAAATCTAAGAGTTATGCTTAAATCTCACTCTTCGACTCTTGTTCTGTTTAGTAACTATTATGTCTCTGACCTATTCTATGAGCTTTTAACTACATTGTTGGATTTCTTTCATAAATATACTCATTTTTGAAATCTTGAACCACTTCTTTTAACAGATTTTGCAATCATGAGAACGAGAAAATGACATTCAAATCTAATGGTTATCCTCTTCTGTTTATGGGATTTTACATTTTGGATTAATCCCATATGTGGCCTCAGTTTTCTATTTCATTTCCAATCTTAAACATCATGCACTGTTCTTTGTTAGGTTTAAGCATGTTTACACGAAAAACACTACATGAGTCCTTCTCTTTCGCTACATTACATTTATGGGTTACTCTGTTTTTTACTGTTTTTTGGTGAAAAGCATTGAAAATCACCAAGTTTTTCTTCATCTATCCTCATGTCAGATCATTTATTTTGAAGGTATATCTTGTCAGATTCAACCATTTATGGAGATGAAAATTTAGATTATATCATCTATGTTTAACGCTAAGTTCCTCTAATCTCTGAAGTAATGGTGTTACCTGTTGCAGGAAAGTGAATGCTTCCTTAACGAGAAGGCAATTGCTCCAAATCTCAGAGAAAGATGAATCTTCTTCTTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGACCGAAATTGGCCAAACAACCGCCAGCGCCTCGCCGATTGCGCTATAGGATTCGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTCACGGACGATTCGGACGACGATGCAGTAAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTGTGGATCGTCTTCCCTGCCAATATGCTCATCAAACTCTCGCAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACGTTGCAATACATAAGTAATGTCATCATCCACAACATCCATATCCACCATTGTCATCCATCAGGTAACAAAATCGACAACCCTAATTTACCTTCCTGCACATCAAATGTTCGACAAAGTGCATCGATCTAAAGCTTGGCTGTGTAGGTAACACCATGGTTCGATCAAGTCCGACGCATTACGGGTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGATCGAAGGACATATGGATTGATCATTGTTCATTGTCTCATTGCAAAGATGGATTGATCGATGCGGTGATGGGATCCACCGGAATCACGATTTCAAACAATTATTTCTCACATCACGATGAAGTTATGTTGTTGGGTCACAGCGACAACTACTGGCCGGATTCCGGTATGCAGGTCAGTGAATTCTCCATCCATTTTCCTTGTTTCTACCCTCATATTCTTACTTAATTCATTCAATTAAAAAATGAATTAACCACAAAGTTCGGTTTATAATAGATTATCTAGGAAATAGGAGAGTTTTGGTGAGTTAAACAAGCACCTTAAAAAATTCATGGGTTCAACTATTAATTTATACTCCAAATTTATAAGTAAGAATGATTTAAACATGAAGGTAACAATAGCATTCAACCACTTCGGAGAAAAGCTAGTGCAAAGGATGCCACGCTGCCGATTAGGTTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGCAACCCCACCATCAACAGCCAAGGCAACCGTTACACCGCCCCATATGACCGCAACGCCAAGGAGGTCACCAAGCGAGTCGAAACTTCCGAATCCGAGTGGCGCGGCTGGAACTGGCGCTCCGAGGGAGACATCCTTGTGAACGGCGCCTTCTTCGTCACTTCCGGTCAAGGGCTTGAAGTCAAGTACGAGAAGGCTTACAGCGTCGAGCCGAAGTCCGCGGCGCTGATTGATCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGATAATAATTTGGGGATGTGGACGACTGGCCCTAATGGTAATGGCGGTGGGTTTGGGCTTGGGTCTGGTATGGACTATACTGACGACATGTCGGAGAGTTGTTTTGTAAGGGTGTTTTCTGTTTTGTTGTGGTCTTTTTGTTGGGCACTGGTCGTGGTGGTTGCGTTTGTGCTATGACGACATGTCGTCAGAGTGTTTGAGAGGATAGAAATGAGAGGTGGAGTGGGTTTTTGCATTTTAATTTAAATGGCATAAAGAAAGGCCTAAAAATGTAAATCCATTTGTATTCCAAAACTGACTTTAGTTTATCTAATTTACTTTTTGTTAAATTGGATATGGAGTTGTTCTTCTTACTTTAGAACTCATACAAAAAAATGCAAAAAAAAAGATATATATATTATCGAAAAGATTGTTGAATTGAATTTCAATTCAGCAGGAAAAAATT

mRNA sequence

ATGCTGGCCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCCGCTTGCCGCTCCGTGCCGCTGCCACCACCTCCGCCGCTCTCAACCTCACTCTCCCTGGCCAGCATCCTAGCCCTGAATTGGTTGCTCAAGAAGTTCACAGGAAAGTGAATGCTTCCTTAACGAGAAGGCAATTGCTCCAAATCTCAGAGAAAGATGAATCTTCTTCTTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGACCGAAATTGGCCAAACAACCGCCAGCGCCTCGCCGATTGCGCTATAGGATTCGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTCACGGACGATTCGGACGACGATGCAGTAAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTGTGGATCGTCTTCCCTGCCAATATGCTCATCAAACTCTCGCAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACGTTGCAATACATAAGTAATGTCATCATCCACAACATCCATATCCACCATTGTCATCCATCAGGTAACACCATGGTTCGATCAAGTCCGACGCATTACGGGTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGATCGAAGGACATATGGATTGATCATTGTTCATTGTCTCATTGCAAAGATGGATTGATCGATGCGGTGATGGGATCCACCGGAATCACGATTTCAAACAATTATTTCTCACATCACGATGAAGTTATGTTGTTGGGTCACAGCGACAACTACTGGCCGGATTCCGGTATGCAGGTAACAATAGCATTCAACCACTTCGGAGAAAAGCTAGTGCAAAGGATGCCACGCTGCCGATTAGGTTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGCAACCCCACCATCAACAGCCAAGGCAACCGTTACACCGCCCCATATGACCGCAACGCCAAGGAGGTCACCAAGCGAGTCGAAACTTCCGAATCCGAGTGGCGCGGCTGGAACTGGCGCTCCGAGGGAGACATCCTTGTGAACGGCGCCTTCTTCGTCACTTCCGGTCAAGGGCTTGAAGTCAAGTACGAGAAGGCTTACAGCGTCGAGCCGAAGTCCGCGGCGCTGATTGATCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGATAATAATTTGGGGATGTGGACGACTGGCCCTAATGGTAATGGCGGTGGGTTTGGGCTTGGGTCTGGTATGGACTATACTGACGACATGTCGGAGAGTTGTTTTGTAAGGGTGTTTTCTGTTTTGTTGTGGTCTTTTTGTTGGGCACTGGTCGTGGTGGTTGCGTTTGTGCTATGA

Coding sequence (CDS)

ATGCTGGCCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCCGCTTGCCGCTCCGTGCCGCTGCCACCACCTCCGCCGCTCTCAACCTCACTCTCCCTGGCCAGCATCCTAGCCCTGAATTGGTTGCTCAAGAAGTTCACAGGAAAGTGAATGCTTCCTTAACGAGAAGGCAATTGCTCCAAATCTCAGAGAAAGATGAATCTTCTTCTTGCTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGACCGAAATTGGCCAAACAACCGCCAGCGCCTCGCCGATTGCGCTATAGGATTCGGCCAGTACGCTCTCGGCGGCAAGAACGGCGAGTTCTACATAGTCACGGACGATTCGGACGACGATGCAGTAAATCCAAAGCCCGGAACATTACGATACGCCGTGATACAGCCGCAACCTCTGTGGATCGTCTTCCCTGCCAATATGCTCATCAAACTCTCGCAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACGTTGCAATACATAAGTAATGTCATCATCCACAACATCCATATCCACCATTGTCATCCATCAGGTAACACCATGGTTCGATCAAGTCCGACGCATTACGGGTACCGGACGAAATCGGACGGCGACGGGATATCGATCTTCGGATCGAAGGACATATGGATTGATCATTGTTCATTGTCTCATTGCAAAGATGGATTGATCGATGCGGTGATGGGATCCACCGGAATCACGATTTCAAACAATTATTTCTCACATCACGATGAAGTTATGTTGTTGGGTCACAGCGACAACTACTGGCCGGATTCCGGTATGCAGGTAACAATAGCATTCAACCACTTCGGAGAAAAGCTAGTGCAAAGGATGCCACGCTGCCGATTAGGTTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGCAACCCCACCATCAACAGCCAAGGCAACCGTTACACCGCCCCATATGACCGCAACGCCAAGGAGGTCACCAAGCGAGTCGAAACTTCCGAATCCGAGTGGCGCGGCTGGAACTGGCGCTCCGAGGGAGACATCCTTGTGAACGGCGCCTTCTTCGTCACTTCCGGTCAAGGGCTTGAAGTCAAGTACGAGAAGGCTTACAGCGTCGAGCCGAAGTCCGCGGCGCTGATTGATCAGCTCACTTGGCACGCCGGCCCGCTCGGCGTCGGCGGTAGGGATAATAATTTGGGGATGTGGACGACTGGCCCTAATGGTAATGGCGGTGGGTTTGGGCTTGGGTCTGGTATGGACTATACTGACGACATGTCGGAGAGTTGTTTTGTAAGGGTGTTTTCTGTTTTGTTGTGGTCTTTTTGTTGGGCACTGGTCGTGGTGGTTGCGTTTGTGCTATGA

Protein sequence

MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL*
Homology
BLAST of CsaV3_6G040000 vs. NCBI nr
Match: XP_004143279.1 (probable pectate lyase 12 [Cucumis sativus] >KGN48197.1 hypothetical protein Csa_004385 [Cucumis sativus])

HSP 1 Score: 994.2 bits (2569), Expect = 3.9e-286
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60
           MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60

Query: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360
           VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS
Sbjct: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL 480
           GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL
Sbjct: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL 480

BLAST of CsaV3_6G040000 vs. NCBI nr
Match: XP_008462493.1 (PREDICTED: probable pectate lyase 12 [Cucumis melo])

HSP 1 Score: 965.3 bits (2494), Expect = 2.0e-277
Identity = 471/482 (97.72%), Postives = 474/482 (98.34%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAAT--TSAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60
           MLATTCIVLLALLLPSRLPLRA AT  T+AALNLTLPGQHPSPELVAQEVHRKVNASLTR
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAGATTATAAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60

Query: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120
           RQLLQISEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIV
Sbjct: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120

Query: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180
           TDDSDDDAVNPK GTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV
Sbjct: 121 TDDSDDDAVNPKLGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180

Query: 181 GGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240
           GGGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID
Sbjct: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240

Query: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300
           HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE
Sbjct: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300

Query: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360
           KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE
Sbjct: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360

Query: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420
           TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG
Sbjct: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420

Query: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAF 480
           VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVR FSVLL SFCW LVVV+AF
Sbjct: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRAFSVLL-SFCWGLVVVIAF 480

BLAST of CsaV3_6G040000 vs. NCBI nr
Match: KAA0063799.1 (putative pectate lyase 12 [Cucumis melo var. makuwa] >TYK07325.1 putative pectate lyase 12 [Cucumis melo var. makuwa])

HSP 1 Score: 964.9 bits (2493), Expect = 2.6e-277
Identity = 470/482 (97.51%), Postives = 474/482 (98.34%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAAT--TSAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60
           MLATTCIVLLALLLPSRLP RA AT  T+AALNLTLPGQHPSPELVAQEVHRKVNASLTR
Sbjct: 1   MLATTCIVLLALLLPSRLPHRAGATTATAAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60

Query: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120
           RQLLQISEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIV
Sbjct: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120

Query: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180
           TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV
Sbjct: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180

Query: 181 GGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240
           GGGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID
Sbjct: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240

Query: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300
           HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE
Sbjct: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300

Query: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360
           KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE
Sbjct: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360

Query: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420
           TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG
Sbjct: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420

Query: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAF 480
           VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMS+SCFVR FSVLL SFCW LVVV+AF
Sbjct: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSKSCFVRAFSVLL-SFCWGLVVVIAF 480

BLAST of CsaV3_6G040000 vs. NCBI nr
Match: XP_038880992.1 (probable pectate lyase 12 [Benincasa hispida])

HSP 1 Score: 917.1 bits (2369), Expect = 6.1e-263
Identity = 453/481 (94.18%), Postives = 461/481 (95.84%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSA-ALNLTLPGQHPSPELVAQEVHRKVNASLTRR 60
           MLATT IVLLALLLP+ LPLRA+A  +A ALNLTLPGQHPSPELVAQEVHRKVNASLTRR
Sbjct: 1   MLATTYIVLLALLLPTSLPLRASAGAAAVALNLTLPGQHPSPELVAQEVHRKVNASLTRR 60

Query: 61  QLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVT 120
           QLLQISEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIVT
Sbjct: 61  QLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVT 120

Query: 121 DDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVG 180
           DDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVG
Sbjct: 121 DDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVG 180

Query: 181 GGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDH 240
           GGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDH
Sbjct: 181 GGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDH 240

Query: 241 CSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEK 300
           CSLS CKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+Y PDSGMQVTIAFNHFGEK
Sbjct: 241 CSLSRCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDHYLPDSGMQVTIAFNHFGEK 300

Query: 301 LVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET 360
           LVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET
Sbjct: 301 LVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET 360

Query: 361 SESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGV 420
           SESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGV
Sbjct: 361 SESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGV 420

Query: 421 GGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFV 480
           GGRDNNLGMWTTGP  NGGGF LGSGMDYTDDMSES FVR FS+LL  FC    +VVAF+
Sbjct: 421 GGRDNNLGMWTTGP--NGGGFALGSGMDYTDDMSESYFVRAFSLLLACFC---ALVVAFM 476

BLAST of CsaV3_6G040000 vs. NCBI nr
Match: XP_022925720.1 (probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate lyase 12 [Cucurbita moschata])

HSP 1 Score: 894.8 bits (2311), Expect = 3.2e-256
Identity = 445/486 (91.56%), Postives = 453/486 (93.21%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60
           ML TTCIVLLALLLPS  PL AAA   AALNLTLPGQHPSPELVAQEVHRKVNAS+TRRQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAA---AALNLTLPGQHPSPELVAQEVHRKVNASVTRRQ 60

Query: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQ  EKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+Y PDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360
           VQRMPRCR GYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVETS
Sbjct: 301 VQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESC-------FVRVFSVLLWSFCWALV 480
           GRDNNLGMWTTGPNG GGGF +GSG DYTDDMSES        FV V SVLL SFC    
Sbjct: 421 GRDNNLGMWTTGPNG-GGGFVIGSGPDYTDDMSESLSRIGSPYFVMVLSVLLCSFC---A 479

BLAST of CsaV3_6G040000 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 721.5 bits (1861), Expect = 6.4e-207
Identity = 354/457 (77.46%), Postives = 390/457 (85.34%), Query Frame = 0

Query: 1   MLATTCIVL---LALLLPSRLPLRAAATTSAALNLT-LPGQHPSPELVAQEVHRKVNASL 60
           ML  +CIVL   L LL+P  +         + LN T L   HP PELVA +V  KVNAS+
Sbjct: 2   MLQRSCIVLFFSLFLLVPQMV--------FSMLNRTLLLIPHPDPELVAYQVQWKVNASI 61

Query: 61  TRRQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFY 120
           TRRQ L  +++  S+ C TGNPIDDCWKCD NWPNNRQ LADC IGFGQYALGGK G+FY
Sbjct: 62  TRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFY 121

Query: 121 IVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVH 180
            VTD SDDDAVNPKPGTLRY VIQ +PLWIVFP+NM+IKL QELIFNSYKTLDGRGANVH
Sbjct: 122 FVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVH 181

Query: 181 IVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIW 240
           IVGGGCITLQY+SN+IIHNIHIHHC+ SGNT VRSSPTHYG+RTKSDGDGISIFGSKDIW
Sbjct: 182 IVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIW 241

Query: 241 IDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHF 300
           IDHCSLS CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD+Y PDSGMQVTIAFNHF
Sbjct: 242 IDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHF 301

Query: 301 GEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKR 360
           GEKL+QRMPRCR GYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAP +  AKEVTKR
Sbjct: 302 GEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKR 361

Query: 361 VETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGP 420
           VET + +W+GWNWRSEGDILVNGAFFV SG+G E++YEKAYSVEPKSA+ I Q+T+H+G 
Sbjct: 362 VETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGV 421

Query: 421 LGVGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMS 454
           LGVGGR+NNLGMWTT   G+ G  GL S  DYTD+MS
Sbjct: 422 LGVGGRNNNLGMWTT--TGSEGTSGLDSYNDYTDEMS 448

BLAST of CsaV3_6G040000 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.4e-161
Identity = 279/424 (65.80%), Postives = 323/424 (76.18%), Query Frame = 0

Query: 30  LNLTLPGQHPSPELVAQEVHRKVNASLTRRQL------LQISEKDESSSCYTGNPIDDCW 89
           LNLTLP QHPSP+ VA  V R VN SL RRQL         S    SSSC TGNPIDDCW
Sbjct: 26  LNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPIDDCW 85

Query: 90  KC-DRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQ 149
           +C D +W  NRQRLADC+IGFG   LGGKNG+ Y+VTD SD++  NP PGTLRY VIQ +
Sbjct: 86  RCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEE 145

Query: 150 PLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCH 209
           PLWIVF +NMLI+L QELI NSYKTLDGRG+ VHI G GC+TLQY+ ++IIHN+HI+ C 
Sbjct: 146 PLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCK 205

Query: 210 PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITIS 269
           PS           +  R +SDGDGISIFGS+ IW+DHCS+SHC DGLIDAVMGST ITIS
Sbjct: 206 PSAG---------FEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTAITIS 265

Query: 270 NNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQW 329
           NNYF+HHDEVMLLGH DNY PD+GMQVTIAFNHFG+ LVQRMPRCR GYIHVVNNDFT+W
Sbjct: 266 NNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEW 325

Query: 330 EMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET-SESEWRGWNWRSEGDILVNGAF 389
           +MYAIGGSGNPTINSQGNRY+AP D +AKEVTKRV++  + EW  WNWR+EGD++ NGAF
Sbjct: 326 KMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLMENGAF 385

Query: 390 FVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGNGGGFG 446
           FV SG+G+   Y KA SV+PK+A+L+DQLT +AG  G    D      +  P G  GG G
Sbjct: 386 FVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPYGGDGGGG 440

BLAST of CsaV3_6G040000 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.1e-153
Identity = 252/385 (65.45%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 41  PELVAQEVHRKVNASLTRRQLLQISEKDESS-------SCYTGNPIDDCWKCDRNWPNNR 100
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CDRNW  NR
Sbjct: 89  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 148

Query: 101 QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANML 160
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 149 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 208

Query: 161 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSP 220
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 209 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 268

Query: 221 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 280
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 269 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 328

Query: 281 LLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNP 340
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 329 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 388

Query: 341 TINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKY 400
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRSEGD+L+NGAFF  SG G    Y
Sbjct: 389 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 448

Query: 401 EKAYSVEPKSAALIDQLTWHAGPLG 419
            +A S+  K ++++D +T  AG LG
Sbjct: 449 GRASSLAAKPSSMVDTITSTAGALG 463

BLAST of CsaV3_6G040000 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.6e-151
Identity = 248/392 (63.27%), Postives = 302/392 (77.04%), Query Frame = 0

Query: 28  AALNLTLPGQHPSPELVAQEVHRKVNASLTRRQLLQISEKDESSSCYTGNPIDDCWKCDR 87
           +AL  +LP     PELV +EVHRK+N S++RR+L          SC +GNPIDDCW+CD+
Sbjct: 42  SALASSLPVS--DPELVVEEVHRKINESISRRKL-------GFFSCGSGNPIDDCWRCDK 101

Query: 88  NWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIV 147
           +W  NR+RLADC IGFG+ A+GG++GE Y+VTD  +DD VNP+PGTLRYAVIQ +PLWI+
Sbjct: 102 DWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWII 161

Query: 148 FPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNT 207
           F  +M I+L +ELI NS+KTLDGRGA+VHI GG CIT+QY++N+IIH +HIH C   GNT
Sbjct: 162 FKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNT 221

Query: 208 MVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFS 267
            VR SP HYGYRT SDGDG+SIFG   +W+DHCSLS+C DGLIDA+ GST ITISNNY +
Sbjct: 222 YVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLT 281

Query: 268 HHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAI 327
           HH++VMLLGHSD Y  D  MQVTIAFNHFGE LVQRMPRCR GY HVVNND+T WEMYAI
Sbjct: 282 HHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 341

Query: 328 GGSGNPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQ 387
           GGS NPTINSQGNR+ AP D ++KEVTK  +  E EWR WNWRSEGD+L+NGAFF  SG 
Sbjct: 342 GGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGA 401

Query: 388 G--LEVKYEKAYSVEPKSAALIDQLTWHAGPL 418
           G      Y KA S+  + ++ + ++T  +G L
Sbjct: 402 GPAKSSSYSKASSLAARPSSHVGEITIASGAL 424

BLAST of CsaV3_6G040000 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.0e-148
Identity = 246/384 (64.06%), Postives = 298/384 (77.60%), Query Frame = 0

Query: 40  SPELVAQEVHRKVNASLTRRQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADC 99
           +PE VA  V   +  S  RR+L          SC TGNPIDDCW+CDR W + R+ LA+C
Sbjct: 39  NPEEVAAMVDMSIRNSTARRRLGYF-------SCSTGNPIDDCWRCDRRWQSRRKHLANC 98

Query: 100 AIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQE 159
           AIGFG+ A+GG++G +Y+V+D +DD+ VNPKPGTLR+AVIQ +PLWIVF  +M+I L +E
Sbjct: 99  AIGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEE 158

Query: 160 LIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYR 219
           LI NS+KT+DGRG NVHI  G CIT+Q+++N+IIH IHIH C P+GN MVRSSP+HYG+R
Sbjct: 159 LIMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWR 218

Query: 220 TKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD 279
           T +DGDGISIFGS  IWIDH SLS+C DGLIDAVM ST ITISNNYF+HH+EVMLLGHSD
Sbjct: 219 TMADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSD 278

Query: 280 NYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQG 339
            Y  D  MQVTIA+NHFGE L+QRMPRCR GY HVVNND+T WEMYAIGGS +PTINSQG
Sbjct: 279 TYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQG 338

Query: 340 NRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSV 399
           NRY AP +R AKEVTKR    + +WR WNWRSEGD+ +NGAFF  SG GL   Y +A S+
Sbjct: 339 NRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSL 398

Query: 400 EPKSAALIDQLTWHAGPLGV-GGR 423
             KS++L+  +T++AG L   GGR
Sbjct: 399 AAKSSSLVGVITYNAGALNCRGGR 415

BLAST of CsaV3_6G040000 vs. ExPASy TrEMBL
Match: A0A0A0KIG0 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 1.9e-286
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60
           MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60

Query: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360
           VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS
Sbjct: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL 480
           GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL
Sbjct: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAFVL 480

BLAST of CsaV3_6G040000 vs. ExPASy TrEMBL
Match: A0A1S3CH45 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103500832 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 9.5e-278
Identity = 471/482 (97.72%), Postives = 474/482 (98.34%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAAT--TSAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60
           MLATTCIVLLALLLPSRLPLRA AT  T+AALNLTLPGQHPSPELVAQEVHRKVNASLTR
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAGATTATAAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60

Query: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120
           RQLLQISEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIV
Sbjct: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120

Query: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180
           TDDSDDDAVNPK GTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV
Sbjct: 121 TDDSDDDAVNPKLGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180

Query: 181 GGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240
           GGGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID
Sbjct: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240

Query: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300
           HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE
Sbjct: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300

Query: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360
           KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE
Sbjct: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360

Query: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420
           TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG
Sbjct: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420

Query: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAF 480
           VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVR FSVLL SFCW LVVV+AF
Sbjct: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRAFSVLL-SFCWGLVVVIAF 480

BLAST of CsaV3_6G040000 vs. ExPASy TrEMBL
Match: A0A5A7VE54 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00400 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.2e-277
Identity = 470/482 (97.51%), Postives = 474/482 (98.34%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAAT--TSAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60
           MLATTCIVLLALLLPSRLP RA AT  T+AALNLTLPGQHPSPELVAQEVHRKVNASLTR
Sbjct: 1   MLATTCIVLLALLLPSRLPHRAGATTATAAALNLTLPGQHPSPELVAQEVHRKVNASLTR 60

Query: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120
           RQLLQISEKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIV
Sbjct: 61  RQLLQISEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIV 120

Query: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180
           TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV
Sbjct: 121 TDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIV 180

Query: 181 GGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240
           GGGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID
Sbjct: 181 GGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWID 240

Query: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300
           HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE
Sbjct: 241 HCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGE 300

Query: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360
           KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE
Sbjct: 301 KLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVE 360

Query: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420
           TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG
Sbjct: 361 TSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG 420

Query: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESCFVRVFSVLLWSFCWALVVVVAF 480
           VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMS+SCFVR FSVLL SFCW LVVV+AF
Sbjct: 421 VGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSKSCFVRAFSVLL-SFCWGLVVVIAF 480

BLAST of CsaV3_6G040000 vs. ExPASy TrEMBL
Match: A0A6J1EJ04 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 445/486 (91.56%), Postives = 453/486 (93.21%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60
           ML TTCIVLLALLLPS  PL AAA   AALNLTLPGQHPSPELVAQEVHRKVNAS+TRRQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAA---AALNLTLPGQHPSPELVAQEVHRKVNASVTRRQ 60

Query: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQ  EKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+Y PDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360
           VQRMPRCR GYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVETS
Sbjct: 301 VQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESC-------FVRVFSVLLWSFCWALV 480
           GRDNNLGMWTTGPNG GGGF +GSG DYTDDMSES        FV V SVLL SFC    
Sbjct: 421 GRDNNLGMWTTGPNG-GGGFVIGSGPDYTDDMSESLSRIGSPYFVMVLSVLLCSFC---A 479

BLAST of CsaV3_6G040000 vs. ExPASy TrEMBL
Match: A0A6J1IRN3 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 442/486 (90.95%), Postives = 453/486 (93.21%), Query Frame = 0

Query: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQ 60
           ML TTCIVLLALLLPS  PL A+A  +AALNLTLPGQHPSPELVAQEVHRKVNAS+TRRQ
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGASA--AAALNLTLPGQHPSPELVAQEVHRKVNASVTRRQ 60

Query: 61  LLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120
           LLQ  EKDES SCYTGNPIDDCWKCD NWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD
Sbjct: 61  LLQSLEKDESPSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTD 120

Query: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGG 180
           DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL QELIFNSYKTLDGRGANVHIVGG
Sbjct: 121 DSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGG 180

Query: 181 GCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHC 240
           GCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKD+WIDHC
Sbjct: 181 GCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDVWIDHC 240

Query: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKL 300
           SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+Y PDSGMQVTIAFNHFGEKL
Sbjct: 241 SLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKL 300

Query: 301 VQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVETS 360
           VQRMPRCR GYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYTAPYDRNAKEVTKRVETS
Sbjct: 301 VQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETS 360

Query: 361 ESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVG 420
           ESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVEPKS ALIDQLTWHAGPLGVG
Sbjct: 361 ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVG 420

Query: 421 GRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESC-------FVRVFSVLLWSFCWALV 480
           GRDNNLGMWTTGPNG GGGF +GSG DYTDDMSES        FV V SVLL SFC    
Sbjct: 421 GRDNNLGMWTTGPNG-GGGFIIGSGPDYTDDMSESLSRIGSPYFVMVVSVLLCSFC---A 480

BLAST of CsaV3_6G040000 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 721.5 bits (1861), Expect = 4.6e-208
Identity = 354/457 (77.46%), Postives = 390/457 (85.34%), Query Frame = 0

Query: 1   MLATTCIVL---LALLLPSRLPLRAAATTSAALNLT-LPGQHPSPELVAQEVHRKVNASL 60
           ML  +CIVL   L LL+P  +         + LN T L   HP PELVA +V  KVNAS+
Sbjct: 2   MLQRSCIVLFFSLFLLVPQMV--------FSMLNRTLLLIPHPDPELVAYQVQWKVNASI 61

Query: 61  TRRQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFY 120
           TRRQ L  +++  S+ C TGNPIDDCWKCD NWPNNRQ LADC IGFGQYALGGK G+FY
Sbjct: 62  TRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFY 121

Query: 121 IVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVH 180
            VTD SDDDAVNPKPGTLRY VIQ +PLWIVFP+NM+IKL QELIFNSYKTLDGRGANVH
Sbjct: 122 FVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVH 181

Query: 181 IVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIW 240
           IVGGGCITLQY+SN+IIHNIHIHHC+ SGNT VRSSPTHYG+RTKSDGDGISIFGSKDIW
Sbjct: 182 IVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIW 241

Query: 241 IDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHF 300
           IDHCSLS CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD+Y PDSGMQVTIAFNHF
Sbjct: 242 IDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHF 301

Query: 301 GEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKR 360
           GEKL+QRMPRCR GYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAP +  AKEVTKR
Sbjct: 302 GEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKR 361

Query: 361 VETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGP 420
           VET + +W+GWNWRSEGDILVNGAFFV SG+G E++YEKAYSVEPKSA+ I Q+T+H+G 
Sbjct: 362 VETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGV 421

Query: 421 LGVGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMS 454
           LGVGGR+NNLGMWTT   G+ G  GL S  DYTD+MS
Sbjct: 422 LGVGGRNNNLGMWTT--TGSEGTSGLDSYNDYTDEMS 448

BLAST of CsaV3_6G040000 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 596.7 bits (1537), Expect = 1.7e-170
Identity = 292/463 (63.07%), Postives = 346/463 (74.73%), Query Frame = 0

Query: 6   CIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVHRKVNASLTRRQLLQIS 65
           CI+   LL+      RA++T+++  NL+LP QHP PE V   V RK+N SL+RRQLL   
Sbjct: 18  CIIWFCLLVSLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQRKLNDSLSRRQLLTYQ 77

Query: 66  EKDESS------SCYTGNPIDDCWKCDRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVT 125
           + D ++      SC TGNPIDDCW+CD NW  NRQRLADC+IGFGQ  LGGK G+FY+VT
Sbjct: 78  QDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADCSIGFGQGTLGGKGGQFYLVT 137

Query: 126 DDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVG 185
           D SD+DA NP PGTLR+AVIQP+PLWI+F ++M IKL  ELI  SYKT+DGRG N+ I G
Sbjct: 138 DSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHELIIGSYKTIDGRGTNIQITG 197

Query: 186 GGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDH 245
            GC+T+Q +S+VIIHN+HIHHC PSGNT+V SSPTH G+R  SDGDGIS+  S  IW+DH
Sbjct: 198 HGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFRGVSDGDGISVSASHHIWVDH 257

Query: 246 CSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEK 305
           CSL +C DGLID ++ ST +TISNNYFSHHDEVMLLGH D Y  D GMQVTIAFNHFGE 
Sbjct: 258 CSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDDRYTADKGMQVTIAFNHFGEG 317

Query: 306 LVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET 365
           LVQRMPRCR GYIHVVNNDFT WEMYAIGGS +PTINSQGNRYTAP D NAKEVTKRV++
Sbjct: 318 LVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQGNRYTAPIDPNAKEVTKRVDS 377

Query: 366 SESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLG- 425
           +E  W GWNWR+EGD++VNGAFFV SG G+   Y +A SV+PK+AA+IDQLT +AG  G 
Sbjct: 378 NEKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFGD 437

Query: 426 VGGRDNNLGMWTTGPNGNG---------GGFGLGSGMDYTDDM 453
             GR+   G +    NG G         G  G GSG D  D +
Sbjct: 438 PSGRNGQGGSFPGITNGGGTITRGYSKSGPAGGGSGSDSDDGL 480

BLAST of CsaV3_6G040000 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 570.1 bits (1468), Expect = 1.7e-162
Identity = 279/424 (65.80%), Postives = 323/424 (76.18%), Query Frame = 0

Query: 30  LNLTLPGQHPSPELVAQEVHRKVNASLTRRQL------LQISEKDESSSCYTGNPIDDCW 89
           LNLTLP QHPSP+ VA  V R VN SL RRQL         S    SSSC TGNPIDDCW
Sbjct: 26  LNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPIDDCW 85

Query: 90  KC-DRNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQ 149
           +C D +W  NRQRLADC+IGFG   LGGKNG+ Y+VTD SD++  NP PGTLRY VIQ +
Sbjct: 86  RCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEE 145

Query: 150 PLWIVFPANMLIKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCH 209
           PLWIVF +NMLI+L QELI NSYKTLDGRG+ VHI G GC+TLQY+ ++IIHN+HI+ C 
Sbjct: 146 PLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCK 205

Query: 210 PSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITIS 269
           PS           +  R +SDGDGISIFGS+ IW+DHCS+SHC DGLIDAVMGST ITIS
Sbjct: 206 PSAG---------FEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTAITIS 265

Query: 270 NNYFSHHDEVMLLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQW 329
           NNYF+HHDEVMLLGH DNY PD+GMQVTIAFNHFG+ LVQRMPRCR GYIHVVNNDFT+W
Sbjct: 266 NNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEW 325

Query: 330 EMYAIGGSGNPTINSQGNRYTAPYDRNAKEVTKRVET-SESEWRGWNWRSEGDILVNGAF 389
           +MYAIGGSGNPTINSQGNRY+AP D +AKEVTKRV++  + EW  WNWR+EGD++ NGAF
Sbjct: 326 KMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLMENGAF 385

Query: 390 FVTSGQGLEVKYEKAYSVEPKSAALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGNGGGFG 446
           FV SG+G+   Y KA SV+PK+A+L+DQLT +AG  G    D      +  P G  GG G
Sbjct: 386 FVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPYGGDGGGG 440

BLAST of CsaV3_6G040000 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.2e-154
Identity = 252/385 (65.45%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 41  PELVAQEVHRKVNASLTRRQLLQISEKDESS-------SCYTGNPIDDCWKCDRNWPNNR 100
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CDRNW  NR
Sbjct: 89  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 148

Query: 101 QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANML 160
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 149 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 208

Query: 161 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSP 220
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 209 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 268

Query: 221 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 280
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 269 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 328

Query: 281 LLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNP 340
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 329 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 388

Query: 341 TINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKY 400
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRSEGD+L+NGAFF  SG G    Y
Sbjct: 389 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 448

Query: 401 EKAYSVEPKSAALIDQLTWHAGPLG 419
            +A S+  K ++++D +T  AG LG
Sbjct: 449 GRASSLAAKPSSMVDTITSTAGALG 463

BLAST of CsaV3_6G040000 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.2e-154
Identity = 252/385 (65.45%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 41  PELVAQEVHRKVNASLTRRQLLQISEKDESS-------SCYTGNPIDDCWKCDRNWPNNR 100
           P++VA EV           +L+Q+SE++ ++       SC TGNPIDDCW+CDRNW  NR
Sbjct: 70  PDMVADEV----------AKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNR 129

Query: 101 QRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANML 160
           +RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKPGTLR+AVIQ +PLWIVF  +M+
Sbjct: 130 KRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMV 189

Query: 161 IKLSQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSP 220
           I+L QELI NS+KT+D RG+NVHI  G CIT+Q+I+NVIIH +HIH C P+GN MVRSSP
Sbjct: 190 IELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSP 249

Query: 221 THYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVM 280
           +H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+DAVMGST IT+SNN+F+HH+EVM
Sbjct: 250 SHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVM 309

Query: 281 LLGHSDNYWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNP 340
           LLGHSD+Y  D  MQVTIA+NHFGE LVQRMPRCR GY HVVNND+T WEMYAIGGS  P
Sbjct: 310 LLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEP 369

Query: 341 TINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKY 400
           TINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRSEGD+L+NGAFF  SG G    Y
Sbjct: 370 TINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASY 429

Query: 401 EKAYSVEPKSAALIDQLTWHAGPLG 419
            +A S+  K ++++D +T  AG LG
Sbjct: 430 GRASSLAAKPSSMVDTITSTAGALG 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143279.13.9e-286100.00probable pectate lyase 12 [Cucumis sativus] >KGN48197.1 hypothetical protein Csa... [more]
XP_008462493.12.0e-27797.72PREDICTED: probable pectate lyase 12 [Cucumis melo][more]
KAA0063799.12.6e-27797.51putative pectate lyase 12 [Cucumis melo var. makuwa] >TYK07325.1 putative pectat... [more]
XP_038880992.16.1e-26394.18probable pectate lyase 12 [Benincasa hispida][more]
XP_022925720.13.2e-25691.56probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate ... [more]
Match NameE-valueIdentityDescription
Q9SCP26.4e-20777.46Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2[more]
Q93Z042.4e-16165.80Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1[more]
Q944R13.1e-15365.45Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q93Z258.6e-15163.27Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Q93WF13.0e-14864.06Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KIG01.9e-286100.00Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1[more]
A0A1S3CH459.5e-27897.72Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103500832 PE=3 SV=1[more]
A0A5A7VE541.2e-27797.51Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00400 ... [more]
A0A6J1EJ041.6e-25691.56Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1[more]
A0A6J1IRN31.7e-25590.95Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53190.14.6e-20877.46Pectin lyase-like superfamily protein [more]
AT5G04310.11.7e-17063.07Pectin lyase-like superfamily protein [more]
AT3G54920.11.7e-16265.80Pectin lyase-like superfamily protein [more]
AT4G13710.12.2e-15465.45Pectin lyase-like superfamily protein [more]
AT4G13710.22.2e-15465.45Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 99..116
score: 57.67
coord: 325..344
score: 73.33
coord: 367..391
score: 51.24
coord: 159..175
score: 67.79
coord: 396..419
score: 27.38
coord: 223..244
score: 67.53
coord: 303..322
score: 67.38
coord: 123..148
score: 67.22
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 151..348
e-value: 7.1E-93
score: 324.5
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 161..340
e-value: 9.8E-23
score: 80.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 76..418
e-value: 5.0E-116
score: 389.4
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 5..437
NoneNo IPR availablePANTHERPTHR31683:SF21PECTATE LYASE 12-RELATEDcoord: 5..437
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 72..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G040000.1CsaV3_6G040000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0030570 pectate lyase activity